Paraoxonases gene expression and distribution in rats organs treated with atherogenic diet and atorvastatin therapy
Autori
Ninić, AnaSpasojević-Kalimanovska, Vesna
Sopić, Miron
Munjas, Jelena
Bogavac-Stanojević, Nataša
Jelić-Ivanović, Zorana
Kotur-Stevuljević, Jelena
Spasić, Slavica
Crevar-Sakač, Milkica
Milenković, Marina
Vujić, Zorica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Paraoxonases isoenzymes, PON1, PON2 and PON3, have important antioxidative and anti-inflammatory properties in blood and cells. They prevent oxidation of low and high density lipoprotein particles, foam cells formation and development of atherosclerosis. The authors investigated effects of high fat diet and atorvastatin therapy on paraoxonases gene expression levels and distribution in different rat organs. Liver, white adipose tissue (WAT) and aorta were taken from young male Wistar rats that were fed with normal diet (ND), atherogenic diet (AD) and atherogenic diet with 1.14 mg of atorvastatin per kg (ADA). Messenger RNA (mRNA) relative levels of paraoxonase 1 (PON1), paraoxonase 2 (PON2) and paraoxonase 3 (PON3) were measured in rat organs using real-time polymerase chain reaction (PCR). PON1 mRNA expression levels were down-regulated in ADA compared to AD group. ND group had significantly lower PON2 mRNA expression than AD group. PON1 mRNA expression levels were higher in liver tha...n in aorta in group of rats on ND, AD and ADA. PON2 mRNA expression was higher in WAT than in aorta only in ADA group of rats. PON2 and PON3 were significantly higher than PON1 in aorta of rats on each ND, AD or ADA.
Ključne reči:
Paraoxonase / gene expression / atorvastatin / atherogenic diet / ratIzvor:
Indian Journal of Biotechnology, 2018, 17, 2, 217-223Izdavač:
- Natl Inst Science Communication-Niscair, New Delhi
Finansiranje / projekti:
- Interaktivna uloga dislipidemije, oksidativnog stresa i inflamacije u aterosklerozi i drugim bolestima: genetički i biohemijski markeri (RS-MESTD-Basic Research (BR or ON)-175035)
Institucija/grupa
PharmacyTY - JOUR AU - Ninić, Ana AU - Spasojević-Kalimanovska, Vesna AU - Sopić, Miron AU - Munjas, Jelena AU - Bogavac-Stanojević, Nataša AU - Jelić-Ivanović, Zorana AU - Kotur-Stevuljević, Jelena AU - Spasić, Slavica AU - Crevar-Sakač, Milkica AU - Milenković, Marina AU - Vujić, Zorica PY - 2018 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3054 AB - Paraoxonases isoenzymes, PON1, PON2 and PON3, have important antioxidative and anti-inflammatory properties in blood and cells. They prevent oxidation of low and high density lipoprotein particles, foam cells formation and development of atherosclerosis. The authors investigated effects of high fat diet and atorvastatin therapy on paraoxonases gene expression levels and distribution in different rat organs. Liver, white adipose tissue (WAT) and aorta were taken from young male Wistar rats that were fed with normal diet (ND), atherogenic diet (AD) and atherogenic diet with 1.14 mg of atorvastatin per kg (ADA). Messenger RNA (mRNA) relative levels of paraoxonase 1 (PON1), paraoxonase 2 (PON2) and paraoxonase 3 (PON3) were measured in rat organs using real-time polymerase chain reaction (PCR). PON1 mRNA expression levels were down-regulated in ADA compared to AD group. ND group had significantly lower PON2 mRNA expression than AD group. PON1 mRNA expression levels were higher in liver than in aorta in group of rats on ND, AD and ADA. PON2 mRNA expression was higher in WAT than in aorta only in ADA group of rats. PON2 and PON3 were significantly higher than PON1 in aorta of rats on each ND, AD or ADA. PB - Natl Inst Science Communication-Niscair, New Delhi T2 - Indian Journal of Biotechnology T1 - Paraoxonases gene expression and distribution in rats organs treated with atherogenic diet and atorvastatin therapy VL - 17 IS - 2 SP - 217 EP - 223 UR - https://hdl.handle.net/21.15107/rcub_farfar_3054 ER -
@article{ author = "Ninić, Ana and Spasojević-Kalimanovska, Vesna and Sopić, Miron and Munjas, Jelena and Bogavac-Stanojević, Nataša and Jelić-Ivanović, Zorana and Kotur-Stevuljević, Jelena and Spasić, Slavica and Crevar-Sakač, Milkica and Milenković, Marina and Vujić, Zorica", year = "2018", abstract = "Paraoxonases isoenzymes, PON1, PON2 and PON3, have important antioxidative and anti-inflammatory properties in blood and cells. They prevent oxidation of low and high density lipoprotein particles, foam cells formation and development of atherosclerosis. The authors investigated effects of high fat diet and atorvastatin therapy on paraoxonases gene expression levels and distribution in different rat organs. Liver, white adipose tissue (WAT) and aorta were taken from young male Wistar rats that were fed with normal diet (ND), atherogenic diet (AD) and atherogenic diet with 1.14 mg of atorvastatin per kg (ADA). Messenger RNA (mRNA) relative levels of paraoxonase 1 (PON1), paraoxonase 2 (PON2) and paraoxonase 3 (PON3) were measured in rat organs using real-time polymerase chain reaction (PCR). PON1 mRNA expression levels were down-regulated in ADA compared to AD group. ND group had significantly lower PON2 mRNA expression than AD group. PON1 mRNA expression levels were higher in liver than in aorta in group of rats on ND, AD and ADA. PON2 mRNA expression was higher in WAT than in aorta only in ADA group of rats. PON2 and PON3 were significantly higher than PON1 in aorta of rats on each ND, AD or ADA.", publisher = "Natl Inst Science Communication-Niscair, New Delhi", journal = "Indian Journal of Biotechnology", title = "Paraoxonases gene expression and distribution in rats organs treated with atherogenic diet and atorvastatin therapy", volume = "17", number = "2", pages = "217-223", url = "https://hdl.handle.net/21.15107/rcub_farfar_3054" }
Ninić, A., Spasojević-Kalimanovska, V., Sopić, M., Munjas, J., Bogavac-Stanojević, N., Jelić-Ivanović, Z., Kotur-Stevuljević, J., Spasić, S., Crevar-Sakač, M., Milenković, M.,& Vujić, Z.. (2018). Paraoxonases gene expression and distribution in rats organs treated with atherogenic diet and atorvastatin therapy. in Indian Journal of Biotechnology Natl Inst Science Communication-Niscair, New Delhi., 17(2), 217-223. https://hdl.handle.net/21.15107/rcub_farfar_3054
Ninić A, Spasojević-Kalimanovska V, Sopić M, Munjas J, Bogavac-Stanojević N, Jelić-Ivanović Z, Kotur-Stevuljević J, Spasić S, Crevar-Sakač M, Milenković M, Vujić Z. Paraoxonases gene expression and distribution in rats organs treated with atherogenic diet and atorvastatin therapy. in Indian Journal of Biotechnology. 2018;17(2):217-223. https://hdl.handle.net/21.15107/rcub_farfar_3054 .
Ninić, Ana, Spasojević-Kalimanovska, Vesna, Sopić, Miron, Munjas, Jelena, Bogavac-Stanojević, Nataša, Jelić-Ivanović, Zorana, Kotur-Stevuljević, Jelena, Spasić, Slavica, Crevar-Sakač, Milkica, Milenković, Marina, Vujić, Zorica, "Paraoxonases gene expression and distribution in rats organs treated with atherogenic diet and atorvastatin therapy" in Indian Journal of Biotechnology, 17, no. 2 (2018):217-223, https://hdl.handle.net/21.15107/rcub_farfar_3054 .