Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells
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The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested conc...entrations, with a statistical significance (p < 0:05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.
Ključne reči:
Antigens / Cells / Cytology / Damage detection / Dietary supplements / DNA / Hydrogen peroxide / Oxidation / PeroxidesIzvor:
Oxidative Medicine and Cellular Longevity, 2019, 2019Izdavač:
- Hindawi
Finansiranje / projekti:
- Aberacije ćelijskog ciklusa i uticaj oksidativnog stresa na neurodegenerativne procese i malignu transformaciju ćelije (RS-MESTD-Basic Research (BR or ON)-173034)
- hCOMET COST (Action No. CA 15132)
DOI: 10.1155/2019/5039372
ISSN: 1942-0900
WoS: 000499161600016
Scopus: 2-s2.0-85075739394
Institucija/grupa
PharmacyTY - JOUR AU - Živković, Lada AU - Bajić, Vladan AU - Topalović, Dijana AU - Bruić, Marija AU - Spremo-Potparević, Biljana PY - 2019 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3484 AB - The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0:05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro. PB - Hindawi T2 - Oxidative Medicine and Cellular Longevity T1 - Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells VL - 2019 DO - 10.1155/2019/5039372 ER -
@article{ author = "Živković, Lada and Bajić, Vladan and Topalović, Dijana and Bruić, Marija and Spremo-Potparević, Biljana", year = "2019", abstract = "The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0:05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.", publisher = "Hindawi", journal = "Oxidative Medicine and Cellular Longevity", title = "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells", volume = "2019", doi = "10.1155/2019/5039372" }
Živković, L., Bajić, V., Topalović, D., Bruić, M.,& Spremo-Potparević, B.. (2019). Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. in Oxidative Medicine and Cellular Longevity Hindawi., 2019. https://doi.org/10.1155/2019/5039372
Živković L, Bajić V, Topalović D, Bruić M, Spremo-Potparević B. Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. in Oxidative Medicine and Cellular Longevity. 2019;2019. doi:10.1155/2019/5039372 .
Živković, Lada, Bajić, Vladan, Topalović, Dijana, Bruić, Marija, Spremo-Potparević, Biljana, "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells" in Oxidative Medicine and Cellular Longevity, 2019 (2019), https://doi.org/10.1155/2019/5039372 . .