Long-Term Effects of Maternal Deprivation on the Volume of Dopaminergic Nuclei and Number of Dopaminergic Neurons in Substantia Nigra and Ventral Tegmental Area in Rats
Authors
Kapor, SlobodanAksić, Milan
Puškaš, Laslo
Jukić, Marin
Poleksić, Joko
Milosavljević, Filip
Bjelica, Suncica
Filipović, Branislav
Article (Published version)
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Early life adversities leave long-lasting structural and functional consequences on the brain, which may persist later in life. Dopamine is a neurotransmitter that is extremely important in mood and motor control. The aim of this study was to investigate the effect of maternal deprivation during the ninth postnatal day on the volume of dopaminergic nuclei and the number of dopaminergic neurons in adolescence and adulthood. Maternally deprived and control Wistar rats were sacrificed on postnatal day 35 or 60, and the dopaminergic neurons were stained in coronal histological sections of ventral midbrain with the tyrosine hydroxylase antibody. The volume of dopaminergic nuclei and the number of dopaminergic neurons in the substantia nigra (SN) and ventral tegmental area (VTA) were analyzed in three representative coordinates. Maternal deprivation caused weight loss on postnatal day 21 (weaning) and corticosterone blood level elevation on postnatal days 35 and 60 in stressed compared to co...ntrol rats. In maternally deprived animals, the volumes of SN and VTA were increased compared to the controls. This increase was accompanied by an elevation in the number of dopaminergic neurons in both nuclei. Altogether, based on somatic and corticosterone level measurements, maternal deprivation represents a substantial adversity, and the phenotype it causes in adulthood includes increased volume of the dopaminergic nuclei and number of dopaminergic neurons.
Keywords:
dopamine / early life stress / glucocorticoids / substantia nigra / ventral tegmental areaSource:
Frontiers in Neuroanatomy, 2020, 14Publisher:
- Frontiers Media S.A.
Funding / projects:
- Morphological differences in brain of patients with transsexuality and other problems in gender identity and disturbances in developmental psychiatric ilnesses (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41020)
- Structural and biochemical characteristics of synaptic plasticity alterations in psychiatric disorders (RS-MESTD-Basic Research (BR or ON)-175058)
DOI: 10.3389/fnana.2020.578900
ISSN: 1662-5129
WoS: 000587010900001
Scopus: 2-s2.0-85095586989
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PharmacyTY - JOUR AU - Kapor, Slobodan AU - Aksić, Milan AU - Puškaš, Laslo AU - Jukić, Marin AU - Poleksić, Joko AU - Milosavljević, Filip AU - Bjelica, Suncica AU - Filipović, Branislav PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3735 AB - Early life adversities leave long-lasting structural and functional consequences on the brain, which may persist later in life. Dopamine is a neurotransmitter that is extremely important in mood and motor control. The aim of this study was to investigate the effect of maternal deprivation during the ninth postnatal day on the volume of dopaminergic nuclei and the number of dopaminergic neurons in adolescence and adulthood. Maternally deprived and control Wistar rats were sacrificed on postnatal day 35 or 60, and the dopaminergic neurons were stained in coronal histological sections of ventral midbrain with the tyrosine hydroxylase antibody. The volume of dopaminergic nuclei and the number of dopaminergic neurons in the substantia nigra (SN) and ventral tegmental area (VTA) were analyzed in three representative coordinates. Maternal deprivation caused weight loss on postnatal day 21 (weaning) and corticosterone blood level elevation on postnatal days 35 and 60 in stressed compared to control rats. In maternally deprived animals, the volumes of SN and VTA were increased compared to the controls. This increase was accompanied by an elevation in the number of dopaminergic neurons in both nuclei. Altogether, based on somatic and corticosterone level measurements, maternal deprivation represents a substantial adversity, and the phenotype it causes in adulthood includes increased volume of the dopaminergic nuclei and number of dopaminergic neurons. PB - Frontiers Media S.A. T2 - Frontiers in Neuroanatomy T1 - Long-Term Effects of Maternal Deprivation on the Volume of Dopaminergic Nuclei and Number of Dopaminergic Neurons in Substantia Nigra and Ventral Tegmental Area in Rats VL - 14 DO - 10.3389/fnana.2020.578900 ER -
@article{ author = "Kapor, Slobodan and Aksić, Milan and Puškaš, Laslo and Jukić, Marin and Poleksić, Joko and Milosavljević, Filip and Bjelica, Suncica and Filipović, Branislav", year = "2020", abstract = "Early life adversities leave long-lasting structural and functional consequences on the brain, which may persist later in life. Dopamine is a neurotransmitter that is extremely important in mood and motor control. The aim of this study was to investigate the effect of maternal deprivation during the ninth postnatal day on the volume of dopaminergic nuclei and the number of dopaminergic neurons in adolescence and adulthood. Maternally deprived and control Wistar rats were sacrificed on postnatal day 35 or 60, and the dopaminergic neurons were stained in coronal histological sections of ventral midbrain with the tyrosine hydroxylase antibody. The volume of dopaminergic nuclei and the number of dopaminergic neurons in the substantia nigra (SN) and ventral tegmental area (VTA) were analyzed in three representative coordinates. Maternal deprivation caused weight loss on postnatal day 21 (weaning) and corticosterone blood level elevation on postnatal days 35 and 60 in stressed compared to control rats. In maternally deprived animals, the volumes of SN and VTA were increased compared to the controls. This increase was accompanied by an elevation in the number of dopaminergic neurons in both nuclei. Altogether, based on somatic and corticosterone level measurements, maternal deprivation represents a substantial adversity, and the phenotype it causes in adulthood includes increased volume of the dopaminergic nuclei and number of dopaminergic neurons.", publisher = "Frontiers Media S.A.", journal = "Frontiers in Neuroanatomy", title = "Long-Term Effects of Maternal Deprivation on the Volume of Dopaminergic Nuclei and Number of Dopaminergic Neurons in Substantia Nigra and Ventral Tegmental Area in Rats", volume = "14", doi = "10.3389/fnana.2020.578900" }
Kapor, S., Aksić, M., Puškaš, L., Jukić, M., Poleksić, J., Milosavljević, F., Bjelica, S.,& Filipović, B.. (2020). Long-Term Effects of Maternal Deprivation on the Volume of Dopaminergic Nuclei and Number of Dopaminergic Neurons in Substantia Nigra and Ventral Tegmental Area in Rats. in Frontiers in Neuroanatomy Frontiers Media S.A.., 14. https://doi.org/10.3389/fnana.2020.578900
Kapor S, Aksić M, Puškaš L, Jukić M, Poleksić J, Milosavljević F, Bjelica S, Filipović B. Long-Term Effects of Maternal Deprivation on the Volume of Dopaminergic Nuclei and Number of Dopaminergic Neurons in Substantia Nigra and Ventral Tegmental Area in Rats. in Frontiers in Neuroanatomy. 2020;14. doi:10.3389/fnana.2020.578900 .
Kapor, Slobodan, Aksić, Milan, Puškaš, Laslo, Jukić, Marin, Poleksić, Joko, Milosavljević, Filip, Bjelica, Suncica, Filipović, Branislav, "Long-Term Effects of Maternal Deprivation on the Volume of Dopaminergic Nuclei and Number of Dopaminergic Neurons in Substantia Nigra and Ventral Tegmental Area in Rats" in Frontiers in Neuroanatomy, 14 (2020), https://doi.org/10.3389/fnana.2020.578900 . .