DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats
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2021
Authors
Bruić, MarijaGrujić-Milanović, Jelica
Miloradović, Zoran
Jovović, Đurđica
Živković, Lada
Mihailović-Stanojević, Nevena
Karanović, Danijela
Spremo-Potparević, Biljana
Article (Published version)
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Oxidative damage to protein and lipid macromolecules in target organs in hypertension has been recognized as a major factor contributing to cardiovascular, cerebrovascular, and renal diseases. Data on protein and lipid oxidative damage in spontaneously hypertensive rats are numerous, but there is no information on DNA damage in tissues measured by comet assay. The aim of this study was to determine the baseline damage to DNA, protein, and lipid macromolecules in different organs of spontaneously hypertensive rats. Markers of lipid peroxidation, protein oxidation, and DNA damage were measured in blood, heart, kidney, and liver of 24-week-old spontaneously hypertensive rats. Plasma prooxidant and antioxidant status were determined as well. Age-matched normotensive Wistar rats were used as control. A rise in markers of lipid peroxidation and protein oxidation, malondialdehyde, and advanced oxidation protein products, was detected in all tissues of spontaneously hypertensive rats, with par...ticularly high values in the liver. DNA damage, measured by the comet assay, was significantly higher in all the studied tissues of spontaneously hypertensive rats compared to normotensive control, with more severe damage in the cardiac and renal cells. Significant depletion of the plasma antioxidant barrier in spontaneously hypertensive rats was also observed. This study showed increased damage to all macromolecules in all studied samples of spontaneously hypertensive rats in comparison with control Wistar rats.
Keywords:
Oxidative stress / DNA damage / Comet assay / Hypertension / Spontaneously hypertensive ratsSource:
International Journal of Biochemistry and Cell Biology, 2021, 141Publisher:
- Elsevier Ltd
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200161 (University of Belgrade, Faculty of Pharmacy) (RS-MESTD-inst-2020-200161)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200015 (University of Belgrade, Institute for Medical Research) (RS-MESTD-inst-2020-200015)
DOI: 10.1016/j.biocel.2021.106088
ISSN: 1357-2725
WoS: 000710132700001
Scopus: 2-s2.0-85116667715
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PharmacyTY - JOUR AU - Bruić, Marija AU - Grujić-Milanović, Jelica AU - Miloradović, Zoran AU - Jovović, Đurđica AU - Živković, Lada AU - Mihailović-Stanojević, Nevena AU - Karanović, Danijela AU - Spremo-Potparević, Biljana PY - 2021 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3981 AB - Oxidative damage to protein and lipid macromolecules in target organs in hypertension has been recognized as a major factor contributing to cardiovascular, cerebrovascular, and renal diseases. Data on protein and lipid oxidative damage in spontaneously hypertensive rats are numerous, but there is no information on DNA damage in tissues measured by comet assay. The aim of this study was to determine the baseline damage to DNA, protein, and lipid macromolecules in different organs of spontaneously hypertensive rats. Markers of lipid peroxidation, protein oxidation, and DNA damage were measured in blood, heart, kidney, and liver of 24-week-old spontaneously hypertensive rats. Plasma prooxidant and antioxidant status were determined as well. Age-matched normotensive Wistar rats were used as control. A rise in markers of lipid peroxidation and protein oxidation, malondialdehyde, and advanced oxidation protein products, was detected in all tissues of spontaneously hypertensive rats, with particularly high values in the liver. DNA damage, measured by the comet assay, was significantly higher in all the studied tissues of spontaneously hypertensive rats compared to normotensive control, with more severe damage in the cardiac and renal cells. Significant depletion of the plasma antioxidant barrier in spontaneously hypertensive rats was also observed. This study showed increased damage to all macromolecules in all studied samples of spontaneously hypertensive rats in comparison with control Wistar rats. PB - Elsevier Ltd T2 - International Journal of Biochemistry and Cell Biology T1 - DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats VL - 141 DO - 10.1016/j.biocel.2021.106088 ER -
@article{ author = "Bruić, Marija and Grujić-Milanović, Jelica and Miloradović, Zoran and Jovović, Đurđica and Živković, Lada and Mihailović-Stanojević, Nevena and Karanović, Danijela and Spremo-Potparević, Biljana", year = "2021", abstract = "Oxidative damage to protein and lipid macromolecules in target organs in hypertension has been recognized as a major factor contributing to cardiovascular, cerebrovascular, and renal diseases. Data on protein and lipid oxidative damage in spontaneously hypertensive rats are numerous, but there is no information on DNA damage in tissues measured by comet assay. The aim of this study was to determine the baseline damage to DNA, protein, and lipid macromolecules in different organs of spontaneously hypertensive rats. Markers of lipid peroxidation, protein oxidation, and DNA damage were measured in blood, heart, kidney, and liver of 24-week-old spontaneously hypertensive rats. Plasma prooxidant and antioxidant status were determined as well. Age-matched normotensive Wistar rats were used as control. A rise in markers of lipid peroxidation and protein oxidation, malondialdehyde, and advanced oxidation protein products, was detected in all tissues of spontaneously hypertensive rats, with particularly high values in the liver. DNA damage, measured by the comet assay, was significantly higher in all the studied tissues of spontaneously hypertensive rats compared to normotensive control, with more severe damage in the cardiac and renal cells. Significant depletion of the plasma antioxidant barrier in spontaneously hypertensive rats was also observed. This study showed increased damage to all macromolecules in all studied samples of spontaneously hypertensive rats in comparison with control Wistar rats.", publisher = "Elsevier Ltd", journal = "International Journal of Biochemistry and Cell Biology", title = "DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats", volume = "141", doi = "10.1016/j.biocel.2021.106088" }
Bruić, M., Grujić-Milanović, J., Miloradović, Z., Jovović, Đ., Živković, L., Mihailović-Stanojević, N., Karanović, D.,& Spremo-Potparević, B.. (2021). DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats. in International Journal of Biochemistry and Cell Biology Elsevier Ltd., 141. https://doi.org/10.1016/j.biocel.2021.106088
Bruić M, Grujić-Milanović J, Miloradović Z, Jovović Đ, Živković L, Mihailović-Stanojević N, Karanović D, Spremo-Potparević B. DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats. in International Journal of Biochemistry and Cell Biology. 2021;141. doi:10.1016/j.biocel.2021.106088 .
Bruić, Marija, Grujić-Milanović, Jelica, Miloradović, Zoran, Jovović, Đurđica, Živković, Lada, Mihailović-Stanojević, Nevena, Karanović, Danijela, Spremo-Potparević, Biljana, "DNA, protein and lipid oxidative damage in tissues of spontaneously hypertensive versus normotensive rats" in International Journal of Biochemistry and Cell Biology, 141 (2021), https://doi.org/10.1016/j.biocel.2021.106088 . .