Low-lead doses induce oxidative damage in cardiac tissue: Subacute toxicity study in Wistar rats and Benchmark dose modelling
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2022
Authors
Javorac, DraganaTatović, Simona
Anđelković, Milena
Repić, Aleksandra
Baralić, Katarina
Buha-Đorđević, Aleksandra
Mihajlović, Marija
Kotur-Stevuljević, Jelena
Đukić-Ćosić, Danijela
Ćurčić, Marijana
Antonijević, Biljana
Bulat, Zorica
Article (Published version)
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Exposure to toxic metals, including lead (Pb), were found as important risk factor for cardiovascular diseases. The aim of the study was to simulate low-level subacute Pb exposure scenario and to determine redox status, redox scores (OXY-score, damage score and protective score) and copper (Cu), zinc (Zn), iron (Fe) and manganese (Mn) levels in cardiac tissue of Wistar rats. Based on the obtained results we have established dose-toxic response relationship and derived Benchmark dose. The male Wistar rats were divided in seven groups (n = 6), six threated groups that received 0.1; 0.5; 1; 3; 7; 15 mg Pb/kg body weight/day for 28 days, by oral gavage and control group. The results of the presented study demonstrated that Pb affect cardiac tissue by inducing production of superoxide anion radical (O2.-) and consequently raising malondialdehyde (MDA) levels. The positive trend in OXY-score and damage score were determined. Effect size analysis showed that the main toxic effects were oxidat...ive damage and elevation of MDA. The lowest BMD was calculated for MDA (2.2e-0.6 mg Pb/kg b.w./day). Obtained BMD may be useful in further assessing point of departure in the human health risks assessment of low-level Pb exposure scenario.
Keywords:
Oxidative stress / Cardiovascular toxicity / Dose-response / Essential elementsSource:
Food and Chemical Toxicology, 2022, 161Publisher:
- Elsevier Ltd
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200161 (University of Belgrade, Faculty of Pharmacy) (RS-MESTD-inst-2020-200161)
DOI: 10.1016/j.fct.2022.112825
ISSN: 0278-6915
WoS: 000750806600005
Scopus: 2-s2.0-85123121914
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PharmacyTY - JOUR AU - Javorac, Dragana AU - Tatović, Simona AU - Anđelković, Milena AU - Repić, Aleksandra AU - Baralić, Katarina AU - Buha-Đorđević, Aleksandra AU - Mihajlović, Marija AU - Kotur-Stevuljević, Jelena AU - Đukić-Ćosić, Danijela AU - Ćurčić, Marijana AU - Antonijević, Biljana AU - Bulat, Zorica PY - 2022 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4046 AB - Exposure to toxic metals, including lead (Pb), were found as important risk factor for cardiovascular diseases. The aim of the study was to simulate low-level subacute Pb exposure scenario and to determine redox status, redox scores (OXY-score, damage score and protective score) and copper (Cu), zinc (Zn), iron (Fe) and manganese (Mn) levels in cardiac tissue of Wistar rats. Based on the obtained results we have established dose-toxic response relationship and derived Benchmark dose. The male Wistar rats were divided in seven groups (n = 6), six threated groups that received 0.1; 0.5; 1; 3; 7; 15 mg Pb/kg body weight/day for 28 days, by oral gavage and control group. The results of the presented study demonstrated that Pb affect cardiac tissue by inducing production of superoxide anion radical (O2.-) and consequently raising malondialdehyde (MDA) levels. The positive trend in OXY-score and damage score were determined. Effect size analysis showed that the main toxic effects were oxidative damage and elevation of MDA. The lowest BMD was calculated for MDA (2.2e-0.6 mg Pb/kg b.w./day). Obtained BMD may be useful in further assessing point of departure in the human health risks assessment of low-level Pb exposure scenario. PB - Elsevier Ltd T2 - Food and Chemical Toxicology T1 - Low-lead doses induce oxidative damage in cardiac tissue: Subacute toxicity study in Wistar rats and Benchmark dose modelling VL - 161 DO - 10.1016/j.fct.2022.112825 ER -
@article{ author = "Javorac, Dragana and Tatović, Simona and Anđelković, Milena and Repić, Aleksandra and Baralić, Katarina and Buha-Đorđević, Aleksandra and Mihajlović, Marija and Kotur-Stevuljević, Jelena and Đukić-Ćosić, Danijela and Ćurčić, Marijana and Antonijević, Biljana and Bulat, Zorica", year = "2022", abstract = "Exposure to toxic metals, including lead (Pb), were found as important risk factor for cardiovascular diseases. The aim of the study was to simulate low-level subacute Pb exposure scenario and to determine redox status, redox scores (OXY-score, damage score and protective score) and copper (Cu), zinc (Zn), iron (Fe) and manganese (Mn) levels in cardiac tissue of Wistar rats. Based on the obtained results we have established dose-toxic response relationship and derived Benchmark dose. The male Wistar rats were divided in seven groups (n = 6), six threated groups that received 0.1; 0.5; 1; 3; 7; 15 mg Pb/kg body weight/day for 28 days, by oral gavage and control group. The results of the presented study demonstrated that Pb affect cardiac tissue by inducing production of superoxide anion radical (O2.-) and consequently raising malondialdehyde (MDA) levels. The positive trend in OXY-score and damage score were determined. Effect size analysis showed that the main toxic effects were oxidative damage and elevation of MDA. The lowest BMD was calculated for MDA (2.2e-0.6 mg Pb/kg b.w./day). Obtained BMD may be useful in further assessing point of departure in the human health risks assessment of low-level Pb exposure scenario.", publisher = "Elsevier Ltd", journal = "Food and Chemical Toxicology", title = "Low-lead doses induce oxidative damage in cardiac tissue: Subacute toxicity study in Wistar rats and Benchmark dose modelling", volume = "161", doi = "10.1016/j.fct.2022.112825" }
Javorac, D., Tatović, S., Anđelković, M., Repić, A., Baralić, K., Buha-Đorđević, A., Mihajlović, M., Kotur-Stevuljević, J., Đukić-Ćosić, D., Ćurčić, M., Antonijević, B.,& Bulat, Z.. (2022). Low-lead doses induce oxidative damage in cardiac tissue: Subacute toxicity study in Wistar rats and Benchmark dose modelling. in Food and Chemical Toxicology Elsevier Ltd., 161. https://doi.org/10.1016/j.fct.2022.112825
Javorac D, Tatović S, Anđelković M, Repić A, Baralić K, Buha-Đorđević A, Mihajlović M, Kotur-Stevuljević J, Đukić-Ćosić D, Ćurčić M, Antonijević B, Bulat Z. Low-lead doses induce oxidative damage in cardiac tissue: Subacute toxicity study in Wistar rats and Benchmark dose modelling. in Food and Chemical Toxicology. 2022;161. doi:10.1016/j.fct.2022.112825 .
Javorac, Dragana, Tatović, Simona, Anđelković, Milena, Repić, Aleksandra, Baralić, Katarina, Buha-Đorđević, Aleksandra, Mihajlović, Marija, Kotur-Stevuljević, Jelena, Đukić-Ćosić, Danijela, Ćurčić, Marijana, Antonijević, Biljana, Bulat, Zorica, "Low-lead doses induce oxidative damage in cardiac tissue: Subacute toxicity study in Wistar rats and Benchmark dose modelling" in Food and Chemical Toxicology, 161 (2022), https://doi.org/10.1016/j.fct.2022.112825 . .