Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme
Само за регистроване кориснике
2022
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Alchemilla viridiflora Rothm., Rosaceae is a herbaceous plant widespread in central Greece, Bulgaria, North Macedonia and Serbia with Kosovo. Liquid chromatography-mass spectrometry analysis leads to the identification of 20 compounds in methanol extract, mainly ellagitannins and flavonoid glycosides. Given that various plant extracts have traditionally been used to treat hypertension and that some of the analyzed methanol extract constituents have beneficial cardiovascular effects, we hypothesized that some of these effects are achieved by inhibiting angiotensin I-converting enzyme (ACE). The dose- dependent ACE inhibitory activities of A. viridiflora and miquelianin were observed with an IC 50 of 2.51 ± 0.00 μg/mL of A. viridiflora extract compared to the IC 50 of 5.4139 ± 0.00 μM for miquelianin. The contribution of the single compounds to the tested activity was further analyzed through the in silico experimental approach. Computational docking results showed that tiliroside, ellag...ic acid pentose and galloyl-hexahydroxydiphenoyl-glucose exhibited even better binding affinity for the ACE active site than miquelianin, for which ACE activity was confirmed by an in vitro assay.
Кључне речи:
ACE inhibitors / Alchemilla viridiflora / Chemical composition / Ellagitannins / FlavonoidsИзвор:
Molecular and Cellular Biochemistry, 2022, 477, 1893-1903Издавач:
- Springer
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
DOI: 10.1007/s11010-022-04410-7
ISSN: 0300-8177
WoS: 000776379100003
Scopus: 2-s2.0-85129899855
Институција/група
PharmacyTY - JOUR AU - Radović, Jelena AU - Suručić, Relja AU - Niketić, Marjan AU - Kundaković-Vasović, Tatjana PY - 2022 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4114 AB - Alchemilla viridiflora Rothm., Rosaceae is a herbaceous plant widespread in central Greece, Bulgaria, North Macedonia and Serbia with Kosovo. Liquid chromatography-mass spectrometry analysis leads to the identification of 20 compounds in methanol extract, mainly ellagitannins and flavonoid glycosides. Given that various plant extracts have traditionally been used to treat hypertension and that some of the analyzed methanol extract constituents have beneficial cardiovascular effects, we hypothesized that some of these effects are achieved by inhibiting angiotensin I-converting enzyme (ACE). The dose- dependent ACE inhibitory activities of A. viridiflora and miquelianin were observed with an IC 50 of 2.51 ± 0.00 μg/mL of A. viridiflora extract compared to the IC 50 of 5.4139 ± 0.00 μM for miquelianin. The contribution of the single compounds to the tested activity was further analyzed through the in silico experimental approach. Computational docking results showed that tiliroside, ellagic acid pentose and galloyl-hexahydroxydiphenoyl-glucose exhibited even better binding affinity for the ACE active site than miquelianin, for which ACE activity was confirmed by an in vitro assay. PB - Springer T2 - Molecular and Cellular Biochemistry T1 - Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme VL - 477 SP - 1893 EP - 1903 DO - 10.1007/s11010-022-04410-7 ER -
@article{ author = "Radović, Jelena and Suručić, Relja and Niketić, Marjan and Kundaković-Vasović, Tatjana", year = "2022", abstract = "Alchemilla viridiflora Rothm., Rosaceae is a herbaceous plant widespread in central Greece, Bulgaria, North Macedonia and Serbia with Kosovo. Liquid chromatography-mass spectrometry analysis leads to the identification of 20 compounds in methanol extract, mainly ellagitannins and flavonoid glycosides. Given that various plant extracts have traditionally been used to treat hypertension and that some of the analyzed methanol extract constituents have beneficial cardiovascular effects, we hypothesized that some of these effects are achieved by inhibiting angiotensin I-converting enzyme (ACE). The dose- dependent ACE inhibitory activities of A. viridiflora and miquelianin were observed with an IC 50 of 2.51 ± 0.00 μg/mL of A. viridiflora extract compared to the IC 50 of 5.4139 ± 0.00 μM for miquelianin. The contribution of the single compounds to the tested activity was further analyzed through the in silico experimental approach. Computational docking results showed that tiliroside, ellagic acid pentose and galloyl-hexahydroxydiphenoyl-glucose exhibited even better binding affinity for the ACE active site than miquelianin, for which ACE activity was confirmed by an in vitro assay.", publisher = "Springer", journal = "Molecular and Cellular Biochemistry", title = "Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme", volume = "477", pages = "1893-1903", doi = "10.1007/s11010-022-04410-7" }
Radović, J., Suručić, R., Niketić, M.,& Kundaković-Vasović, T.. (2022). Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme. in Molecular and Cellular Biochemistry Springer., 477, 1893-1903. https://doi.org/10.1007/s11010-022-04410-7
Radović J, Suručić R, Niketić M, Kundaković-Vasović T. Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme. in Molecular and Cellular Biochemistry. 2022;477:1893-1903. doi:10.1007/s11010-022-04410-7 .
Radović, Jelena, Suručić, Relja, Niketić, Marjan, Kundaković-Vasović, Tatjana, "Alchemilla viridiflora Rothm.: the potent natural inhibitor of angiotensin I-converting enzyme" in Molecular and Cellular Biochemistry, 477 (2022):1893-1903, https://doi.org/10.1007/s11010-022-04410-7 . .