Lipoproteins and cholesterol homeostasis in paediatric nephrotic syndrome patients
Authors
Simachew, Yonas MulatAntonić, Tamara
Gojković, Tamara
Vladimirov, Sandra
Mihajlović, Marija
Vujčić, Sanja
Miloševski-Lomić, Gordana
Vekić, Jelena
Zeljković, Aleksandra
Spasojević-Kalimanovska, Vesna
Peco-Antić, Amira
Paripović, Dušan
Stefanović, Aleksandra
Article (Published version)
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Introduction The aim of this study was to investigate lipoprotein particle distributions and the likelihood of achieving cholesterol homeostasis in the remission phase of nephrotic syndrome (NS) in paediatric patients. We hypothesized that lipoprotein particle distributions moved toward less atherogenic profile and that cholesterol homeostasis was achieved. Materials and methods Thirty-three children, 2 to 9 years old with NS were recruited. Blood sampling took place both in the acute phase and during remission. Serum low-density lipoprotein particles (LDL) and high-density lipoprotein particles (HDL) were separated using non-denaturing polyacrylamide gradient gel (3-31%) electrophoresis. Serum non-cholesterols sterols (NCSs), desmosterol, lathosterol, 7-dehydrocholesterol (7-DHC), campesterol and β-sitosterol were measured by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Results All patients had desirable serum HDL cholesterol concentrations during r...emission. The dominant lipoprotein diameters and LDL subclass distribution did not change significantly during follow-up. In contrast, HDL lipoprotein particle distribution shifted towards larger particles. The absolute concentration of desmosterol was significantly lower during remission (P = 0.023). β-sitosterol concentration markedly increased during remission (P = 0.005). Desmosterol/β-sitosterol (P < 0.001) and 7-DHC/β-sitosterol (P = 0.005) ratios significantly declined during disease remission. Conclusions Favourable changes in the serum lipid profiles, HDL particle subclass distribution and cholesterol metabolism in paediatric patients with NS during remission took place. For the first time, we found that cholesterol homeostasis changed in favour of increased cholesterol absorption during disease remission. Nevertheless, complete cholesterol homeostasis was not achieved during disease remission.
Keywords:
glucocorticoid therapy / non-cholesterol sterols / paediatric nephrotic syndrome / serum lipid profileSource:
Biochemia Medica, 2022, 32, 2Publisher:
- Croatian Society of Medical Biochemistry and Laboratory Medicine
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200161 (University of Belgrade, Faculty of Pharmacy) (RS-MESTD-inst-2020-200161)
DOI: 10.11613/BM.2022.020706
ISSN: 1330-0962
WoS: 000820264100008
Scopus: 2-s2.0-85133633842
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PharmacyTY - JOUR AU - Simachew, Yonas Mulat AU - Antonić, Tamara AU - Gojković, Tamara AU - Vladimirov, Sandra AU - Mihajlović, Marija AU - Vujčić, Sanja AU - Miloševski-Lomić, Gordana AU - Vekić, Jelena AU - Zeljković, Aleksandra AU - Spasojević-Kalimanovska, Vesna AU - Peco-Antić, Amira AU - Paripović, Dušan AU - Stefanović, Aleksandra PY - 2022 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4195 AB - Introduction The aim of this study was to investigate lipoprotein particle distributions and the likelihood of achieving cholesterol homeostasis in the remission phase of nephrotic syndrome (NS) in paediatric patients. We hypothesized that lipoprotein particle distributions moved toward less atherogenic profile and that cholesterol homeostasis was achieved. Materials and methods Thirty-three children, 2 to 9 years old with NS were recruited. Blood sampling took place both in the acute phase and during remission. Serum low-density lipoprotein particles (LDL) and high-density lipoprotein particles (HDL) were separated using non-denaturing polyacrylamide gradient gel (3-31%) electrophoresis. Serum non-cholesterols sterols (NCSs), desmosterol, lathosterol, 7-dehydrocholesterol (7-DHC), campesterol and β-sitosterol were measured by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Results All patients had desirable serum HDL cholesterol concentrations during remission. The dominant lipoprotein diameters and LDL subclass distribution did not change significantly during follow-up. In contrast, HDL lipoprotein particle distribution shifted towards larger particles. The absolute concentration of desmosterol was significantly lower during remission (P = 0.023). β-sitosterol concentration markedly increased during remission (P = 0.005). Desmosterol/β-sitosterol (P < 0.001) and 7-DHC/β-sitosterol (P = 0.005) ratios significantly declined during disease remission. Conclusions Favourable changes in the serum lipid profiles, HDL particle subclass distribution and cholesterol metabolism in paediatric patients with NS during remission took place. For the first time, we found that cholesterol homeostasis changed in favour of increased cholesterol absorption during disease remission. Nevertheless, complete cholesterol homeostasis was not achieved during disease remission. PB - Croatian Society of Medical Biochemistry and Laboratory Medicine T2 - Biochemia Medica T1 - Lipoproteins and cholesterol homeostasis in paediatric nephrotic syndrome patients VL - 32 IS - 2 DO - 10.11613/BM.2022.020706 ER -
@article{ author = "Simachew, Yonas Mulat and Antonić, Tamara and Gojković, Tamara and Vladimirov, Sandra and Mihajlović, Marija and Vujčić, Sanja and Miloševski-Lomić, Gordana and Vekić, Jelena and Zeljković, Aleksandra and Spasojević-Kalimanovska, Vesna and Peco-Antić, Amira and Paripović, Dušan and Stefanović, Aleksandra", year = "2022", abstract = "Introduction The aim of this study was to investigate lipoprotein particle distributions and the likelihood of achieving cholesterol homeostasis in the remission phase of nephrotic syndrome (NS) in paediatric patients. We hypothesized that lipoprotein particle distributions moved toward less atherogenic profile and that cholesterol homeostasis was achieved. Materials and methods Thirty-three children, 2 to 9 years old with NS were recruited. Blood sampling took place both in the acute phase and during remission. Serum low-density lipoprotein particles (LDL) and high-density lipoprotein particles (HDL) were separated using non-denaturing polyacrylamide gradient gel (3-31%) electrophoresis. Serum non-cholesterols sterols (NCSs), desmosterol, lathosterol, 7-dehydrocholesterol (7-DHC), campesterol and β-sitosterol were measured by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Results All patients had desirable serum HDL cholesterol concentrations during remission. The dominant lipoprotein diameters and LDL subclass distribution did not change significantly during follow-up. In contrast, HDL lipoprotein particle distribution shifted towards larger particles. The absolute concentration of desmosterol was significantly lower during remission (P = 0.023). β-sitosterol concentration markedly increased during remission (P = 0.005). Desmosterol/β-sitosterol (P < 0.001) and 7-DHC/β-sitosterol (P = 0.005) ratios significantly declined during disease remission. Conclusions Favourable changes in the serum lipid profiles, HDL particle subclass distribution and cholesterol metabolism in paediatric patients with NS during remission took place. For the first time, we found that cholesterol homeostasis changed in favour of increased cholesterol absorption during disease remission. Nevertheless, complete cholesterol homeostasis was not achieved during disease remission.", publisher = "Croatian Society of Medical Biochemistry and Laboratory Medicine", journal = "Biochemia Medica", title = "Lipoproteins and cholesterol homeostasis in paediatric nephrotic syndrome patients", volume = "32", number = "2", doi = "10.11613/BM.2022.020706" }
Simachew, Y. M., Antonić, T., Gojković, T., Vladimirov, S., Mihajlović, M., Vujčić, S., Miloševski-Lomić, G., Vekić, J., Zeljković, A., Spasojević-Kalimanovska, V., Peco-Antić, A., Paripović, D.,& Stefanović, A.. (2022). Lipoproteins and cholesterol homeostasis in paediatric nephrotic syndrome patients. in Biochemia Medica Croatian Society of Medical Biochemistry and Laboratory Medicine., 32(2). https://doi.org/10.11613/BM.2022.020706
Simachew YM, Antonić T, Gojković T, Vladimirov S, Mihajlović M, Vujčić S, Miloševski-Lomić G, Vekić J, Zeljković A, Spasojević-Kalimanovska V, Peco-Antić A, Paripović D, Stefanović A. Lipoproteins and cholesterol homeostasis in paediatric nephrotic syndrome patients. in Biochemia Medica. 2022;32(2). doi:10.11613/BM.2022.020706 .
Simachew, Yonas Mulat, Antonić, Tamara, Gojković, Tamara, Vladimirov, Sandra, Mihajlović, Marija, Vujčić, Sanja, Miloševski-Lomić, Gordana, Vekić, Jelena, Zeljković, Aleksandra, Spasojević-Kalimanovska, Vesna, Peco-Antić, Amira, Paripović, Dušan, Stefanović, Aleksandra, "Lipoproteins and cholesterol homeostasis in paediatric nephrotic syndrome patients" in Biochemia Medica, 32, no. 2 (2022), https://doi.org/10.11613/BM.2022.020706 . .