Determination of bifonazole in creams containing methyl- and propyl p-hydroxybenzoate by derivative spectrophotometric method
Abstract
A second order derivative spectrophotometric method for the determination of bifonazole in the presence of methyland propyl p-hydroxybenzoate as preservatives has been developed. The determination was performed in a 0.1 M HCl solution at 241.5 nm, a wavelength corresponding to the intersection of the second order derivative spectra (D-2) of methyl- and propyl p-hydroxybenzoate with the axis (zero-crossing point). On the basis of the knowledge of acidity constants and solubility, as well as of the investigations of zero-order and D-2 spectra of bifonazole and preservatives, these conditions were chosen as optimal ones. A calibration curve constructed for bifonazole concentrations ranging from 1.5 to 15 mug/ml had a correlation coefficient of 0.9998. Reliability and reproducibility of the method was checked by analyzing laboratory mixtures of bifonazole and preservatives (recovery 99.97-102.7%; RDS 0.48-1.46%). The proposed method was applied for the determination of bifonazole in a comm...ercial cream formulation. The mean value of bifonazole obtained per 100 g cream was 1.029 g (102.9% of the labeled claim) with a RSD of 0.60%.
Keywords:
bifonazole / spectrophotometric determination / derivative spectrophotometry / cream formulationSource:
Journal of Pharmaceutical and Biomedical Analysis, 2003, 33, 1, 131-136Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
DOI: 10.1016/S0731-7085(03)00228-0
ISSN: 0731-7085
PubMed: 12946540
WoS: 000186057500016
Scopus: 2-s2.0-0142124310
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Institution/Community
PharmacyTY - JOUR AU - Popović, Gordana AU - Čakar, Mira AU - Agbaba, Danica PY - 2003 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/431 AB - A second order derivative spectrophotometric method for the determination of bifonazole in the presence of methyland propyl p-hydroxybenzoate as preservatives has been developed. The determination was performed in a 0.1 M HCl solution at 241.5 nm, a wavelength corresponding to the intersection of the second order derivative spectra (D-2) of methyl- and propyl p-hydroxybenzoate with the axis (zero-crossing point). On the basis of the knowledge of acidity constants and solubility, as well as of the investigations of zero-order and D-2 spectra of bifonazole and preservatives, these conditions were chosen as optimal ones. A calibration curve constructed for bifonazole concentrations ranging from 1.5 to 15 mug/ml had a correlation coefficient of 0.9998. Reliability and reproducibility of the method was checked by analyzing laboratory mixtures of bifonazole and preservatives (recovery 99.97-102.7%; RDS 0.48-1.46%). The proposed method was applied for the determination of bifonazole in a commercial cream formulation. The mean value of bifonazole obtained per 100 g cream was 1.029 g (102.9% of the labeled claim) with a RSD of 0.60%. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Determination of bifonazole in creams containing methyl- and propyl p-hydroxybenzoate by derivative spectrophotometric method VL - 33 IS - 1 SP - 131 EP - 136 DO - 10.1016/S0731-7085(03)00228-0 ER -
@article{ author = "Popović, Gordana and Čakar, Mira and Agbaba, Danica", year = "2003", abstract = "A second order derivative spectrophotometric method for the determination of bifonazole in the presence of methyland propyl p-hydroxybenzoate as preservatives has been developed. The determination was performed in a 0.1 M HCl solution at 241.5 nm, a wavelength corresponding to the intersection of the second order derivative spectra (D-2) of methyl- and propyl p-hydroxybenzoate with the axis (zero-crossing point). On the basis of the knowledge of acidity constants and solubility, as well as of the investigations of zero-order and D-2 spectra of bifonazole and preservatives, these conditions were chosen as optimal ones. A calibration curve constructed for bifonazole concentrations ranging from 1.5 to 15 mug/ml had a correlation coefficient of 0.9998. Reliability and reproducibility of the method was checked by analyzing laboratory mixtures of bifonazole and preservatives (recovery 99.97-102.7%; RDS 0.48-1.46%). The proposed method was applied for the determination of bifonazole in a commercial cream formulation. The mean value of bifonazole obtained per 100 g cream was 1.029 g (102.9% of the labeled claim) with a RSD of 0.60%.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Determination of bifonazole in creams containing methyl- and propyl p-hydroxybenzoate by derivative spectrophotometric method", volume = "33", number = "1", pages = "131-136", doi = "10.1016/S0731-7085(03)00228-0" }
Popović, G., Čakar, M.,& Agbaba, D.. (2003). Determination of bifonazole in creams containing methyl- and propyl p-hydroxybenzoate by derivative spectrophotometric method. in Journal of Pharmaceutical and Biomedical Analysis Pergamon-Elsevier Science Ltd, Oxford., 33(1), 131-136. https://doi.org/10.1016/S0731-7085(03)00228-0
Popović G, Čakar M, Agbaba D. Determination of bifonazole in creams containing methyl- and propyl p-hydroxybenzoate by derivative spectrophotometric method. in Journal of Pharmaceutical and Biomedical Analysis. 2003;33(1):131-136. doi:10.1016/S0731-7085(03)00228-0 .
Popović, Gordana, Čakar, Mira, Agbaba, Danica, "Determination of bifonazole in creams containing methyl- and propyl p-hydroxybenzoate by derivative spectrophotometric method" in Journal of Pharmaceutical and Biomedical Analysis, 33, no. 1 (2003):131-136, https://doi.org/10.1016/S0731-7085(03)00228-0 . .