Development and validation of LC-MS/MS method for determination of plasma apixaban
Аутори
Džudović, JelenaCrevar-Sakač, Milkica
Antunović, Marko
Repić, Aleksandra
Obradović, Slobodan
Đorđević, Snežana
Savić, Jelena
Džudović, Boris
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Oral anticoagulants are a group of drugs used for the prevention and treatment of venous thrombosis and venous thromboembolism. For the last ten years, direct oral anticoagulants (DOAC) have been available and are equally effective, but significantly safer than vitamin K antagonists. In the case of an overdose, their most important side effect is still bleeding. Due to their widespread use, as well as increased toxicological importance there is a need to develop an analytical method for the determination of DOAC in biological material. The aim of this paper was to establish a method for the quantification of apixaban as one of the representatives of DOAC. The methodology of the study included the measurement of apixaban in the plasma of patients treated in the intensive care unit. Plasma apixaban concentrations were determined by LC-MS/MS technique using carbamazepine as an internal standard. Obtained validation parameters indicate that the introduced method is sensitive, reliable, pre...cise and accurate. Using this method, apixaban can be quickly and easily detected and quantified in plasma in patients who are suspected of overdosing with this drug.
Кључне речи:
anticoagulants / apixaban / DOAC / LC-MS/MSИзвор:
Acta Chromatographica, 2022, 34, 3, 332-337Издавач:
- Akademiai Kiado ZRt.
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
DOI: 10.1556/1326.2021.00948
ISSN: 1233-2356
WoS: 000810400500014
Scopus: 2-s2.0-85134525385
Институција/група
PharmacyTY - JOUR AU - Džudović, Jelena AU - Crevar-Sakač, Milkica AU - Antunović, Marko AU - Repić, Aleksandra AU - Obradović, Slobodan AU - Đorđević, Snežana AU - Savić, Jelena AU - Džudović, Boris PY - 2022 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4332 AB - Oral anticoagulants are a group of drugs used for the prevention and treatment of venous thrombosis and venous thromboembolism. For the last ten years, direct oral anticoagulants (DOAC) have been available and are equally effective, but significantly safer than vitamin K antagonists. In the case of an overdose, their most important side effect is still bleeding. Due to their widespread use, as well as increased toxicological importance there is a need to develop an analytical method for the determination of DOAC in biological material. The aim of this paper was to establish a method for the quantification of apixaban as one of the representatives of DOAC. The methodology of the study included the measurement of apixaban in the plasma of patients treated in the intensive care unit. Plasma apixaban concentrations were determined by LC-MS/MS technique using carbamazepine as an internal standard. Obtained validation parameters indicate that the introduced method is sensitive, reliable, precise and accurate. Using this method, apixaban can be quickly and easily detected and quantified in plasma in patients who are suspected of overdosing with this drug. PB - Akademiai Kiado ZRt. T2 - Acta Chromatographica T1 - Development and validation of LC-MS/MS method for determination of plasma apixaban VL - 34 IS - 3 SP - 332 EP - 337 DO - 10.1556/1326.2021.00948 ER -
@article{ author = "Džudović, Jelena and Crevar-Sakač, Milkica and Antunović, Marko and Repić, Aleksandra and Obradović, Slobodan and Đorđević, Snežana and Savić, Jelena and Džudović, Boris", year = "2022", abstract = "Oral anticoagulants are a group of drugs used for the prevention and treatment of venous thrombosis and venous thromboembolism. For the last ten years, direct oral anticoagulants (DOAC) have been available and are equally effective, but significantly safer than vitamin K antagonists. In the case of an overdose, their most important side effect is still bleeding. Due to their widespread use, as well as increased toxicological importance there is a need to develop an analytical method for the determination of DOAC in biological material. The aim of this paper was to establish a method for the quantification of apixaban as one of the representatives of DOAC. The methodology of the study included the measurement of apixaban in the plasma of patients treated in the intensive care unit. Plasma apixaban concentrations were determined by LC-MS/MS technique using carbamazepine as an internal standard. Obtained validation parameters indicate that the introduced method is sensitive, reliable, precise and accurate. Using this method, apixaban can be quickly and easily detected and quantified in plasma in patients who are suspected of overdosing with this drug.", publisher = "Akademiai Kiado ZRt.", journal = "Acta Chromatographica", title = "Development and validation of LC-MS/MS method for determination of plasma apixaban", volume = "34", number = "3", pages = "332-337", doi = "10.1556/1326.2021.00948" }
Džudović, J., Crevar-Sakač, M., Antunović, M., Repić, A., Obradović, S., Đorđević, S., Savić, J.,& Džudović, B.. (2022). Development and validation of LC-MS/MS method for determination of plasma apixaban. in Acta Chromatographica Akademiai Kiado ZRt.., 34(3), 332-337. https://doi.org/10.1556/1326.2021.00948
Džudović J, Crevar-Sakač M, Antunović M, Repić A, Obradović S, Đorđević S, Savić J, Džudović B. Development and validation of LC-MS/MS method for determination of plasma apixaban. in Acta Chromatographica. 2022;34(3):332-337. doi:10.1556/1326.2021.00948 .
Džudović, Jelena, Crevar-Sakač, Milkica, Antunović, Marko, Repić, Aleksandra, Obradović, Slobodan, Đorđević, Snežana, Savić, Jelena, Džudović, Boris, "Development and validation of LC-MS/MS method for determination of plasma apixaban" in Acta Chromatographica, 34, no. 3 (2022):332-337, https://doi.org/10.1556/1326.2021.00948 . .