ex vivo–in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping
Autori
Krombholz, RichardFressle, Stefanie
Nikolić, Ines
Pantelić, Ivana
Savić, Snežana
Crevar-Sakač, Milkica
Lunter, Dominique
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
When it comes to skin penetration analysis of a topically applied formulation, the number of suitable methods is limited, and they often lack in spatial resolution. In vivo studies are pivotal, especially in the approval of a new product, but high costs and ethical difficulties are limiting factors. For that reason, good ex vivo models for testing skin penetration are crucial. In this study, caffeine was used as a hydrophilic model drug, applied as a 2% (w/w) hydrogel, to compare different techniques for skin penetration analysis. Confocal Raman microspectroscopy (CRM) and tape stripping with subsequent HPLC analysis were used to quantify caffeine. Experiments were performed ex vivo and in vivo. Furthermore, the effect of 5% (w/w) 1,2-pentanediol on caffeine skin penetration was tested, to compare those methods regarding their effectiveness in detecting differences between both formulations.
Ključne reči:
caffeine / confocal Raman spectroscopy / dermatology / drug delivery / skin barrier / skin penetrationIzvor:
Experimental Dermatology, 2022, 31, 12Izdavač:
- John Wiley and Sons Inc
Finansiranje / projekti:
- Ministry of Education, Science and Technological Development, Republic of Serbia (grant numbers 57514345 and 451–03‐01855/2019–09/12).
- Industrial Collective Research, German Federation of Industrial Research Associations (Forschungsvereinigung der Arzneimittelhersteller) and German Federal Ministry for economic affairs and energy
DOI: 10.1111/exd.14672
ISSN: 0906-6705
WoS: 000852120500001
Scopus: 2-s2.0-85137507915
Institucija/grupa
PharmacyTY - JOUR AU - Krombholz, Richard AU - Fressle, Stefanie AU - Nikolić, Ines AU - Pantelić, Ivana AU - Savić, Snežana AU - Crevar-Sakač, Milkica AU - Lunter, Dominique PY - 2022 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4333 AB - When it comes to skin penetration analysis of a topically applied formulation, the number of suitable methods is limited, and they often lack in spatial resolution. In vivo studies are pivotal, especially in the approval of a new product, but high costs and ethical difficulties are limiting factors. For that reason, good ex vivo models for testing skin penetration are crucial. In this study, caffeine was used as a hydrophilic model drug, applied as a 2% (w/w) hydrogel, to compare different techniques for skin penetration analysis. Confocal Raman microspectroscopy (CRM) and tape stripping with subsequent HPLC analysis were used to quantify caffeine. Experiments were performed ex vivo and in vivo. Furthermore, the effect of 5% (w/w) 1,2-pentanediol on caffeine skin penetration was tested, to compare those methods regarding their effectiveness in detecting differences between both formulations. PB - John Wiley and Sons Inc T2 - Experimental Dermatology T1 - ex vivo–in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping VL - 31 IS - 12 DO - 10.1111/exd.14672 ER -
@article{ author = "Krombholz, Richard and Fressle, Stefanie and Nikolić, Ines and Pantelić, Ivana and Savić, Snežana and Crevar-Sakač, Milkica and Lunter, Dominique", year = "2022", abstract = "When it comes to skin penetration analysis of a topically applied formulation, the number of suitable methods is limited, and they often lack in spatial resolution. In vivo studies are pivotal, especially in the approval of a new product, but high costs and ethical difficulties are limiting factors. For that reason, good ex vivo models for testing skin penetration are crucial. In this study, caffeine was used as a hydrophilic model drug, applied as a 2% (w/w) hydrogel, to compare different techniques for skin penetration analysis. Confocal Raman microspectroscopy (CRM) and tape stripping with subsequent HPLC analysis were used to quantify caffeine. Experiments were performed ex vivo and in vivo. Furthermore, the effect of 5% (w/w) 1,2-pentanediol on caffeine skin penetration was tested, to compare those methods regarding their effectiveness in detecting differences between both formulations.", publisher = "John Wiley and Sons Inc", journal = "Experimental Dermatology", title = "ex vivo–in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping", volume = "31", number = "12", doi = "10.1111/exd.14672" }
Krombholz, R., Fressle, S., Nikolić, I., Pantelić, I., Savić, S., Crevar-Sakač, M.,& Lunter, D.. (2022). ex vivo–in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping. in Experimental Dermatology John Wiley and Sons Inc., 31(12). https://doi.org/10.1111/exd.14672
Krombholz R, Fressle S, Nikolić I, Pantelić I, Savić S, Crevar-Sakač M, Lunter D. ex vivo–in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping. in Experimental Dermatology. 2022;31(12). doi:10.1111/exd.14672 .
Krombholz, Richard, Fressle, Stefanie, Nikolić, Ines, Pantelić, Ivana, Savić, Snežana, Crevar-Sakač, Milkica, Lunter, Dominique, "ex vivo–in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping" in Experimental Dermatology, 31, no. 12 (2022), https://doi.org/10.1111/exd.14672 . .