Heracleum ternatum from Mt. Durmitor: furanocoumarins, polyphenols and antioxidant activity of leaf and flower extracts
Апстракт
In this work, composition and antioxidant activity of dry dichloromethane and methanol extracts of Heracleum ternatum Velen. (Apiaceae) leaves and flowers from Mt Durmitor (Montenegro) were investigated. Using LC-MS, five furanocoumarins were identified in leaf dichloromethane extract (accounting for 23.00 mg/g of dry extract) and eight in flower dichloromethane extract (60.47 mg/g). Dominant in leaf extract was heraclenin (11.64 mg/g), followed by imperatorin (5.90 mg/g), and in flower extract heraclenol (17.55 mg/g), followed by heraclenin (15.04 mg/g). LC-MS analysis revealed eight flavonol glycosides in leaf methanol extract (accounting for 16.18 mg/g of dry extract) and 11 in flower methanol extract (65.82 mg/g), as well as chlorogenic acid in both extracts (4.72 mg/g and trace, respectively). Among detected flavonoids, dominant was quercetin 7-O-rhamnosyl 3-O-glucoside (vincetoxicoside A; 7.95 and 16.77 mg/g), followed by biosides and triosides of kaempferol and methylquercetin. ...Content of total polyphenols, determined spectrophotometrically using Folin-Ciocalteu reagent, was 87.29 and 98.12 mg of gallic acid equivalents/g of dry methanol extracts. Among methanol extracts, polyphenol richer flower extract showed higher, spectrophotometrically determined, total antioxidant and anti-DPPH activities compared to leaf extract (FRAP=0.80 and 0.65 mmol Fe2+/g; DPPH SC50=83.12 and 90.95 μg/mL). Dichloromethane extracts showed lower anti-DPPH activity (SC50=860.58 and 515.76 μg/mL).
Извор:
14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts, 2022, 143-143Издавач:
- Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
- Institute for Nature Conservation of Serbia, Belgrade
Финансирање / пројекти:
Напомена:
- 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, 26th to 29th June 2022
Институција/група
PharmacyTY - CONF AU - Ušjak, Ljuboš AU - Drobac, Milica AU - Niketić, Marjan AU - Petrović, Silvana PY - 2022 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4503 AB - In this work, composition and antioxidant activity of dry dichloromethane and methanol extracts of Heracleum ternatum Velen. (Apiaceae) leaves and flowers from Mt Durmitor (Montenegro) were investigated. Using LC-MS, five furanocoumarins were identified in leaf dichloromethane extract (accounting for 23.00 mg/g of dry extract) and eight in flower dichloromethane extract (60.47 mg/g). Dominant in leaf extract was heraclenin (11.64 mg/g), followed by imperatorin (5.90 mg/g), and in flower extract heraclenol (17.55 mg/g), followed by heraclenin (15.04 mg/g). LC-MS analysis revealed eight flavonol glycosides in leaf methanol extract (accounting for 16.18 mg/g of dry extract) and 11 in flower methanol extract (65.82 mg/g), as well as chlorogenic acid in both extracts (4.72 mg/g and trace, respectively). Among detected flavonoids, dominant was quercetin 7-O-rhamnosyl 3-O-glucoside (vincetoxicoside A; 7.95 and 16.77 mg/g), followed by biosides and triosides of kaempferol and methylquercetin. Content of total polyphenols, determined spectrophotometrically using Folin-Ciocalteu reagent, was 87.29 and 98.12 mg of gallic acid equivalents/g of dry methanol extracts. Among methanol extracts, polyphenol richer flower extract showed higher, spectrophotometrically determined, total antioxidant and anti-DPPH activities compared to leaf extract (FRAP=0.80 and 0.65 mmol Fe2+/g; DPPH SC50=83.12 and 90.95 μg/mL). Dichloromethane extracts showed lower anti-DPPH activity (SC50=860.58 and 515.76 μg/mL). PB - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš PB - Institute for Nature Conservation of Serbia, Belgrade C3 - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts T1 - Heracleum ternatum from Mt. Durmitor: furanocoumarins, polyphenols and antioxidant activity of leaf and flower extracts SP - 143 EP - 143 UR - https://hdl.handle.net/21.15107/rcub_farfar_4503 ER -
@conference{ author = "Ušjak, Ljuboš and Drobac, Milica and Niketić, Marjan and Petrović, Silvana", year = "2022", abstract = "In this work, composition and antioxidant activity of dry dichloromethane and methanol extracts of Heracleum ternatum Velen. (Apiaceae) leaves and flowers from Mt Durmitor (Montenegro) were investigated. Using LC-MS, five furanocoumarins were identified in leaf dichloromethane extract (accounting for 23.00 mg/g of dry extract) and eight in flower dichloromethane extract (60.47 mg/g). Dominant in leaf extract was heraclenin (11.64 mg/g), followed by imperatorin (5.90 mg/g), and in flower extract heraclenol (17.55 mg/g), followed by heraclenin (15.04 mg/g). LC-MS analysis revealed eight flavonol glycosides in leaf methanol extract (accounting for 16.18 mg/g of dry extract) and 11 in flower methanol extract (65.82 mg/g), as well as chlorogenic acid in both extracts (4.72 mg/g and trace, respectively). Among detected flavonoids, dominant was quercetin 7-O-rhamnosyl 3-O-glucoside (vincetoxicoside A; 7.95 and 16.77 mg/g), followed by biosides and triosides of kaempferol and methylquercetin. Content of total polyphenols, determined spectrophotometrically using Folin-Ciocalteu reagent, was 87.29 and 98.12 mg of gallic acid equivalents/g of dry methanol extracts. Among methanol extracts, polyphenol richer flower extract showed higher, spectrophotometrically determined, total antioxidant and anti-DPPH activities compared to leaf extract (FRAP=0.80 and 0.65 mmol Fe2+/g; DPPH SC50=83.12 and 90.95 μg/mL). Dichloromethane extracts showed lower anti-DPPH activity (SC50=860.58 and 515.76 μg/mL).", publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade", journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts", title = "Heracleum ternatum from Mt. Durmitor: furanocoumarins, polyphenols and antioxidant activity of leaf and flower extracts", pages = "143-143", url = "https://hdl.handle.net/21.15107/rcub_farfar_4503" }
Ušjak, L., Drobac, M., Niketić, M.,& Petrović, S.. (2022). Heracleum ternatum from Mt. Durmitor: furanocoumarins, polyphenols and antioxidant activity of leaf and flower extracts. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 143-143. https://hdl.handle.net/21.15107/rcub_farfar_4503
Ušjak L, Drobac M, Niketić M, Petrović S. Heracleum ternatum from Mt. Durmitor: furanocoumarins, polyphenols and antioxidant activity of leaf and flower extracts. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:143-143. https://hdl.handle.net/21.15107/rcub_farfar_4503 .
Ušjak, Ljuboš, Drobac, Milica, Niketić, Marjan, Petrović, Silvana, "Heracleum ternatum from Mt. Durmitor: furanocoumarins, polyphenols and antioxidant activity of leaf and flower extracts" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):143-143, https://hdl.handle.net/21.15107/rcub_farfar_4503 .