Composition and antimicrobial activity of essential oil from the underground parts of Laserpitium zernyi Hayek (Apiaceae)
Аутори
Popović, VišnjaPetrović, Silvana
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Pavlović, Milica
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Milenković, Marina
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Couladis, Maria
Tzakou, Olga
Duraki, Šemija
Niketić, Marjan
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Конференцијски прилог (Објављена верзија)
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Метаподаци
Приказ свих података о документуАпстракт
Laserpitium zernyi Hayek is Scardo-Pindic endemic plant distributed in the mountain regions of
C. Balkans. It was treated earlier as a subspecies, L. siler L. subsp. zernyi (Hayek) Tutin [1,2]. Root of L.
siler is traditionally used as tonic, diuretic, emenagogue, in gynecology and externally for toothache treatments [3].
Composition and antimicrobial activity of essential oil from the underground parts (roots and rhizomes)
of L. zernyi were investigated. Air-dried and powdered plant material was hydrodistilled using n-hexane
as a collecting solvent. Pale blue oil yielded 1.2l% (w/w).
The essential oil was analyzed by GC/FID and GC/MS. Forty-three compounds were identified (94.3% of total oil). Oil was characterised by similar content of monoterpenes and sesquiterpenes (52.9% and 41.4%, respectively). The main constituents were α-pinene (3 1.6%) and α-bisabolol (30.9%).
The antimicrobial activity was tested using the microdillution method [4] against Gram(+) bacteria Staphylococ...cus aureus ATCC 25923, S. epidermidis ATCC 12228, Micrococcus luteus ATCC 9341, Enterococcus faecalis ATCC 29212, Gram(-) bacteria Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae NCIMB 9111, Escherichia coli ATCC 25922, and two strains of yeast Candida albicans (ATCC 10259 and 24433).
Laserpitium zernyi oil showed significant antibacterial activity against Staphylococcus epidermidis (MIC 31.83 µg/ml), S. aureus and Micrococcus luteus (MICs 63.67 µg/ml). For all other tested microorganisms MIC values were higher than 100.00 µg /ml.
The studied essential oil isolated from the underground parts of L. zernyi had a remarkably different composition than the previously tested oils from flower and herb of this plant, and also showed a much higher antimicrobial activity [5].
Кључне речи:
Laserpitium zernyi / essential oil / α-pinene / α-bisabolol / antimicrobial activityИзвор:
5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts, 2009, 149-149Издавач:
- Faculty o f Biology, University of Belgrade
Финансирање / пројекти:
- Испитивање лековитог потенцијала биљака: морфолошка, хемијска и фармаколошка карактеризација (RS-MESTD-MPN2006-2010-143012)
Напомена:
- The 80th anniversary of the publication of Turrill’s “Plant life of the Balkan peninsula”
Институција/група
PharmacyTY - CONF AU - Popović, Višnja AU - Petrović, Silvana AU - Pavlović, Milica AU - Milenković, Marina AU - Couladis, Maria AU - Tzakou, Olga AU - Duraki, Šemija AU - Niketić, Marjan PY - 2009 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5160 AB - Laserpitium zernyi Hayek is Scardo-Pindic endemic plant distributed in the mountain regions of C. Balkans. It was treated earlier as a subspecies, L. siler L. subsp. zernyi (Hayek) Tutin [1,2]. Root of L. siler is traditionally used as tonic, diuretic, emenagogue, in gynecology and externally for toothache treatments [3]. Composition and antimicrobial activity of essential oil from the underground parts (roots and rhizomes) of L. zernyi were investigated. Air-dried and powdered plant material was hydrodistilled using n-hexane as a collecting solvent. Pale blue oil yielded 1.2l% (w/w). The essential oil was analyzed by GC/FID and GC/MS. Forty-three compounds were identified (94.3% of total oil). Oil was characterised by similar content of monoterpenes and sesquiterpenes (52.9% and 41.4%, respectively). The main constituents were α-pinene (3 1.6%) and α-bisabolol (30.9%). The antimicrobial activity was tested using the microdillution method [4] against Gram(+) bacteria Staphylococcus aureus ATCC 25923, S. epidermidis ATCC 12228, Micrococcus luteus ATCC 9341, Enterococcus faecalis ATCC 29212, Gram(-) bacteria Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae NCIMB 9111, Escherichia coli ATCC 25922, and two strains of yeast Candida albicans (ATCC 10259 and 24433). Laserpitium zernyi oil showed significant antibacterial activity against Staphylococcus epidermidis (MIC 31.83 µg/ml), S. aureus and Micrococcus luteus (MICs 63.67 µg/ml). For all other tested microorganisms MIC values were higher than 100.00 µg /ml. The studied essential oil isolated from the underground parts of L. zernyi had a remarkably different composition than the previously tested oils from flower and herb of this plant, and also showed a much higher antimicrobial activity [5]. PB - Faculty o f Biology, University of Belgrade C3 - 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts T1 - Composition and antimicrobial activity of essential oil from the underground parts of Laserpitium zernyi Hayek (Apiaceae) SP - 149 EP - 149 UR - https://hdl.handle.net/21.15107/rcub_farfar_5160 ER -
@conference{ author = "Popović, Višnja and Petrović, Silvana and Pavlović, Milica and Milenković, Marina and Couladis, Maria and Tzakou, Olga and Duraki, Šemija and Niketić, Marjan", year = "2009", abstract = "Laserpitium zernyi Hayek is Scardo-Pindic endemic plant distributed in the mountain regions of C. Balkans. It was treated earlier as a subspecies, L. siler L. subsp. zernyi (Hayek) Tutin [1,2]. Root of L. siler is traditionally used as tonic, diuretic, emenagogue, in gynecology and externally for toothache treatments [3]. Composition and antimicrobial activity of essential oil from the underground parts (roots and rhizomes) of L. zernyi were investigated. Air-dried and powdered plant material was hydrodistilled using n-hexane as a collecting solvent. Pale blue oil yielded 1.2l% (w/w). The essential oil was analyzed by GC/FID and GC/MS. Forty-three compounds were identified (94.3% of total oil). Oil was characterised by similar content of monoterpenes and sesquiterpenes (52.9% and 41.4%, respectively). The main constituents were α-pinene (3 1.6%) and α-bisabolol (30.9%). The antimicrobial activity was tested using the microdillution method [4] against Gram(+) bacteria Staphylococcus aureus ATCC 25923, S. epidermidis ATCC 12228, Micrococcus luteus ATCC 9341, Enterococcus faecalis ATCC 29212, Gram(-) bacteria Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae NCIMB 9111, Escherichia coli ATCC 25922, and two strains of yeast Candida albicans (ATCC 10259 and 24433). Laserpitium zernyi oil showed significant antibacterial activity against Staphylococcus epidermidis (MIC 31.83 µg/ml), S. aureus and Micrococcus luteus (MICs 63.67 µg/ml). For all other tested microorganisms MIC values were higher than 100.00 µg /ml. The studied essential oil isolated from the underground parts of L. zernyi had a remarkably different composition than the previously tested oils from flower and herb of this plant, and also showed a much higher antimicrobial activity [5].", publisher = "Faculty o f Biology, University of Belgrade", journal = "5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts", title = "Composition and antimicrobial activity of essential oil from the underground parts of Laserpitium zernyi Hayek (Apiaceae)", pages = "149-149", url = "https://hdl.handle.net/21.15107/rcub_farfar_5160" }
Popović, V., Petrović, S., Pavlović, M., Milenković, M., Couladis, M., Tzakou, O., Duraki, Š.,& Niketić, M.. (2009). Composition and antimicrobial activity of essential oil from the underground parts of Laserpitium zernyi Hayek (Apiaceae). in 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts Faculty o f Biology, University of Belgrade., 149-149. https://hdl.handle.net/21.15107/rcub_farfar_5160
Popović V, Petrović S, Pavlović M, Milenković M, Couladis M, Tzakou O, Duraki Š, Niketić M. Composition and antimicrobial activity of essential oil from the underground parts of Laserpitium zernyi Hayek (Apiaceae). in 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts. 2009;:149-149. https://hdl.handle.net/21.15107/rcub_farfar_5160 .
Popović, Višnja, Petrović, Silvana, Pavlović, Milica, Milenković, Marina, Couladis, Maria, Tzakou, Olga, Duraki, Šemija, Niketić, Marjan, "Composition and antimicrobial activity of essential oil from the underground parts of Laserpitium zernyi Hayek (Apiaceae)" in 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts (2009):149-149, https://hdl.handle.net/21.15107/rcub_farfar_5160 .