Preliminary analysis of specialised metabolites of Cytisus jankae flowering aerial part
2018
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Cytisus jankae Velen. (Fabaceae) is a dwarfish shrub (10–25
cm height) with 3-foliolate leaves and capitate inflorescence
with tubular calyces. The whole plant is densely covered with
silky hairs. Its range includes central and east part of the Bal-
kan Peninsula and Romania. It inhabits dry rocky places on
limestone or ultramafic soils. The objective of this study was
to establish the phytochemical profile of flowering aerial parts
of this plant. The plant material was collected in eastern Ser-
bia (Jelašnička Klisura gorge). In the air-dried herb, by the
direct gravimetric method, the total alkaloids content (0.32%)
was determined, and using colorimetric assays the total poly-
phenols (43.8%), tannins (1.13%), and the total flavonoids
(1.31%) were quantified. Additionally, the powdered dried
plant material was successively extracted with dichlorometh-
ane (maceration) and methanol (bimaceration) at room tem-
perature. After the solvents were evaporated under reduced
p...ressure, dried extracts were subjected to the further analy-
sis. The total alkaloids content in both the dichloromethane
(7.01%) and methanol (3.47%) extracts, and the contents of
total polyphenols (13.75%), tannins (2.45%) and flavonoids
(4.65%) in the methanol extract, were determined by afore-
mentioned tests. Applying the LC-MS method, in the metha-
nol extract flavonoids quercetin 3-O-rutinoside, luteolin, api-
genin and genistein were identified. GC-MS analysis resulted
in the identification of quinolizidine alkaloids sparteine,
17-oxosparteine and lupanine (2-oxosparteine) in both the
methanol and dichloromethane extracts, as well as of one tri-
terpene, β-amyrin, in the dichloromethane extract. This is the
first report on specialised metabolites from flowering aerial
parts of C. jankae.
Ključne reči:
Cytisus jankae / flowering aerial parts / flavonoids / LC-MS / alkaloids / GC-MSIzvor:
Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 2018, 42, 1 supplement, 143-143Izdavač:
- Institute of Botany and Botanical Garden Jevremovac, Belgrade
Finansiranje / projekti:
- Ispitivanje lekovitog potencijala biljaka: morfološka, hemijska i farmakološka karakterizacija (RS-MESTD-Basic Research (BR or ON)-173021)
Napomena:
- 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia
Institucija/grupa
PharmacyTY - CONF AU - Milutinović, Violeta AU - Niketić, Marjan AU - Petrović, Silvana PY - 2018 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5236 AB - Cytisus jankae Velen. (Fabaceae) is a dwarfish shrub (10–25 cm height) with 3-foliolate leaves and capitate inflorescence with tubular calyces. The whole plant is densely covered with silky hairs. Its range includes central and east part of the Bal- kan Peninsula and Romania. It inhabits dry rocky places on limestone or ultramafic soils. The objective of this study was to establish the phytochemical profile of flowering aerial parts of this plant. The plant material was collected in eastern Ser- bia (Jelašnička Klisura gorge). In the air-dried herb, by the direct gravimetric method, the total alkaloids content (0.32%) was determined, and using colorimetric assays the total poly- phenols (43.8%), tannins (1.13%), and the total flavonoids (1.31%) were quantified. Additionally, the powdered dried plant material was successively extracted with dichlorometh- ane (maceration) and methanol (bimaceration) at room tem- perature. After the solvents were evaporated under reduced pressure, dried extracts were subjected to the further analy- sis. The total alkaloids content in both the dichloromethane (7.01%) and methanol (3.47%) extracts, and the contents of total polyphenols (13.75%), tannins (2.45%) and flavonoids (4.65%) in the methanol extract, were determined by afore- mentioned tests. Applying the LC-MS method, in the metha- nol extract flavonoids quercetin 3-O-rutinoside, luteolin, api- genin and genistein were identified. GC-MS analysis resulted in the identification of quinolizidine alkaloids sparteine, 17-oxosparteine and lupanine (2-oxosparteine) in both the methanol and dichloromethane extracts, as well as of one tri- terpene, β-amyrin, in the dichloromethane extract. This is the first report on specialised metabolites from flowering aerial parts of C. jankae. PB - Institute of Botany and Botanical Garden Jevremovac, Belgrade C3 - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts T1 - Preliminary analysis of specialised metabolites of Cytisus jankae flowering aerial part VL - 42 IS - 1 supplement SP - 143 EP - 143 UR - https://hdl.handle.net/21.15107/rcub_farfar_5236 ER -
@conference{ author = "Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana", year = "2018", abstract = "Cytisus jankae Velen. (Fabaceae) is a dwarfish shrub (10–25 cm height) with 3-foliolate leaves and capitate inflorescence with tubular calyces. The whole plant is densely covered with silky hairs. Its range includes central and east part of the Bal- kan Peninsula and Romania. It inhabits dry rocky places on limestone or ultramafic soils. The objective of this study was to establish the phytochemical profile of flowering aerial parts of this plant. The plant material was collected in eastern Ser- bia (Jelašnička Klisura gorge). In the air-dried herb, by the direct gravimetric method, the total alkaloids content (0.32%) was determined, and using colorimetric assays the total poly- phenols (43.8%), tannins (1.13%), and the total flavonoids (1.31%) were quantified. Additionally, the powdered dried plant material was successively extracted with dichlorometh- ane (maceration) and methanol (bimaceration) at room tem- perature. After the solvents were evaporated under reduced pressure, dried extracts were subjected to the further analy- sis. The total alkaloids content in both the dichloromethane (7.01%) and methanol (3.47%) extracts, and the contents of total polyphenols (13.75%), tannins (2.45%) and flavonoids (4.65%) in the methanol extract, were determined by afore- mentioned tests. Applying the LC-MS method, in the metha- nol extract flavonoids quercetin 3-O-rutinoside, luteolin, api- genin and genistein were identified. GC-MS analysis resulted in the identification of quinolizidine alkaloids sparteine, 17-oxosparteine and lupanine (2-oxosparteine) in both the methanol and dichloromethane extracts, as well as of one tri- terpene, β-amyrin, in the dichloromethane extract. This is the first report on specialised metabolites from flowering aerial parts of C. jankae.", publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade", journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts", title = "Preliminary analysis of specialised metabolites of Cytisus jankae flowering aerial part", volume = "42", number = "1 supplement", pages = "143-143", url = "https://hdl.handle.net/21.15107/rcub_farfar_5236" }
Milutinović, V., Niketić, M.,& Petrović, S.. (2018). Preliminary analysis of specialised metabolites of Cytisus jankae flowering aerial part. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 143-143. https://hdl.handle.net/21.15107/rcub_farfar_5236
Milutinović V, Niketić M, Petrović S. Preliminary analysis of specialised metabolites of Cytisus jankae flowering aerial part. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):143-143. https://hdl.handle.net/21.15107/rcub_farfar_5236 .
Milutinović, Violeta, Niketić, Marjan, Petrović, Silvana, "Preliminary analysis of specialised metabolites of Cytisus jankae flowering aerial part" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):143-143, https://hdl.handle.net/21.15107/rcub_farfar_5236 .