New approach in quantitative estimation of x chromosome centromere instability in Alzheimer disease
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Background: Chromosome instability (CIN) in Alzheimer’s disease has been found in peripheral blood lymphocytes, and also in neurons of affected individuals. CIN is a result of errors in chromosome segregation during mitosis leading to numerical and structural chromosomal abnormalities. Material and Methods: Fluorescent In Situ Hybridization. (FISH) has been a method of choice for the evaluation of CIN in various diseases. We have studied a specific type of X chromosome instability, or premature centromere division (PCD) in interphase nuclei of the hippocampus brain tissue neurons from sporadic AD females. PCD was studied by using a new approach to FISH. Results: Namely, we have established a methodology that allows quantitative analysis of centromere fluorescence intensity, i.e. the size of individual FISH spots, thus giving a more focused view of the centromere region of interest. By using the micro image system, our results show that quantitative FISH can distinguish PCD positive sig...nals vs. PCD negative signals in cells of brain tissue. Conclusion: This could be a specific biomarker in affected cells in order to verify CIN, thus supporting the established qualitative analysis of FISH spots in the study of centromere and chromosomal alterations in interphase nuclei of patients affected by AD.
Keywords:
Chromosome instrability / Alzheimer disease / X chromosome / premature centromere division biomarkerSource:
14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK, 2023, 26, Supplement, 88-88Publisher:
- Macedonian Academy of Sciences and Arts
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- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200161 (University of Belgrade, Faculty of Pharmacy) (RS-MESTD-inst-2020-200161)
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PharmacyTY - CONF AU - Spremo-Potparević, Biljana AU - Želježić, Davor AU - Bajić, Vladan AU - Popović, Petar AU - Živković, Lada PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5471 AB - Background: Chromosome instability (CIN) in Alzheimer’s disease has been found in peripheral blood lymphocytes, and also in neurons of affected individuals. CIN is a result of errors in chromosome segregation during mitosis leading to numerical and structural chromosomal abnormalities. Material and Methods: Fluorescent In Situ Hybridization. (FISH) has been a method of choice for the evaluation of CIN in various diseases. We have studied a specific type of X chromosome instability, or premature centromere division (PCD) in interphase nuclei of the hippocampus brain tissue neurons from sporadic AD females. PCD was studied by using a new approach to FISH. Results: Namely, we have established a methodology that allows quantitative analysis of centromere fluorescence intensity, i.e. the size of individual FISH spots, thus giving a more focused view of the centromere region of interest. By using the micro image system, our results show that quantitative FISH can distinguish PCD positive signals vs. PCD negative signals in cells of brain tissue. Conclusion: This could be a specific biomarker in affected cells in order to verify CIN, thus supporting the established qualitative analysis of FISH spots in the study of centromere and chromosomal alterations in interphase nuclei of patients affected by AD. PB - Macedonian Academy of Sciences and Arts C3 - 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK T1 - New approach in quantitative estimation of x chromosome centromere instability in Alzheimer disease VL - 26 IS - Supplement SP - 88 EP - 88 UR - https://hdl.handle.net/21.15107/rcub_farfar_5471 ER -
@conference{ author = "Spremo-Potparević, Biljana and Želježić, Davor and Bajić, Vladan and Popović, Petar and Živković, Lada", year = "2023", abstract = "Background: Chromosome instability (CIN) in Alzheimer’s disease has been found in peripheral blood lymphocytes, and also in neurons of affected individuals. CIN is a result of errors in chromosome segregation during mitosis leading to numerical and structural chromosomal abnormalities. Material and Methods: Fluorescent In Situ Hybridization. (FISH) has been a method of choice for the evaluation of CIN in various diseases. We have studied a specific type of X chromosome instability, or premature centromere division (PCD) in interphase nuclei of the hippocampus brain tissue neurons from sporadic AD females. PCD was studied by using a new approach to FISH. Results: Namely, we have established a methodology that allows quantitative analysis of centromere fluorescence intensity, i.e. the size of individual FISH spots, thus giving a more focused view of the centromere region of interest. By using the micro image system, our results show that quantitative FISH can distinguish PCD positive signals vs. PCD negative signals in cells of brain tissue. Conclusion: This could be a specific biomarker in affected cells in order to verify CIN, thus supporting the established qualitative analysis of FISH spots in the study of centromere and chromosomal alterations in interphase nuclei of patients affected by AD.", publisher = "Macedonian Academy of Sciences and Arts", journal = "14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK", title = "New approach in quantitative estimation of x chromosome centromere instability in Alzheimer disease", volume = "26", number = "Supplement", pages = "88-88", url = "https://hdl.handle.net/21.15107/rcub_farfar_5471" }
Spremo-Potparević, B., Želježić, D., Bajić, V., Popović, P.,& Živković, L.. (2023). New approach in quantitative estimation of x chromosome centromere instability in Alzheimer disease. in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK Macedonian Academy of Sciences and Arts., 26(Supplement), 88-88. https://hdl.handle.net/21.15107/rcub_farfar_5471
Spremo-Potparević B, Želježić D, Bajić V, Popović P, Živković L. New approach in quantitative estimation of x chromosome centromere instability in Alzheimer disease. in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK. 2023;26(Supplement):88-88. https://hdl.handle.net/21.15107/rcub_farfar_5471 .
Spremo-Potparević, Biljana, Želježić, Davor, Bajić, Vladan, Popović, Petar, Živković, Lada, "New approach in quantitative estimation of x chromosome centromere instability in Alzheimer disease" in 14th Balkan Congress o14th Balkan Congress of Human Genetics & 9th Rare Disease SEE Meeting 2023 Supplement Skopje, October 05-07, ABSTRACT BOOK, 26, no. Supplement (2023):88-88, https://hdl.handle.net/21.15107/rcub_farfar_5471 .