Comparison of capillary zone electrophoresis and high performance liquid chromatography methods for quantitative determination of ketoconazole in drug formulations
Abstract
A capillary zone electrophoresis (CZE) and a high-performance liquid chromatography (HPLC) method have been developed for identification and determination of ketoconazole, an imidazole antifungal, in pharmaceutical preparations. The suitabilities of both methods for quantitative determination of ketoconazole were approved through validation specification such as linearity, precision, accuracy, limit of detection and quantification. The proposed methods were used for determination of ketoconazole in commercial pharmaceutical dosage forms (tablets and creams). Under described conditions, CZE method is more selective, while the HPLC method is more sensitive. Both methods are rapid (t R (CZE)=5.14 min and t R (HPLC)=2.66 min), which is important for routine application. However, the HPLC method provides a repeatability of the quantitative analysis of ketoconazole in drug formulations below 1.5% relative standard deviation (R.S.D), while the repeatability of the CZE method is in the order o...f 2-3% R.S.D.
Keywords:
Capillary electrophoresis / High performance liquid chromatography / Ketoconazole / Method validationSource:
Farmaco, 2004, 59, 5, 419-424Publisher:
- Elsevier Masson SAS
Note:
- Ministry of Science, Technology and Development of the Republic of Serbia (Project no. 1458 - Molecular structures, chemical transformations, physicochemical characterization, pharmaceutical purity and analysis of pharmacologicaly active compounds).
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Institution/Community
PharmacyTY - JOUR AU - Velikinac, I AU - Čudina, Olivera AU - Janković, I AU - Agbaba, Danica AU - Vladimirov, S PY - 2004 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/554 AB - A capillary zone electrophoresis (CZE) and a high-performance liquid chromatography (HPLC) method have been developed for identification and determination of ketoconazole, an imidazole antifungal, in pharmaceutical preparations. The suitabilities of both methods for quantitative determination of ketoconazole were approved through validation specification such as linearity, precision, accuracy, limit of detection and quantification. The proposed methods were used for determination of ketoconazole in commercial pharmaceutical dosage forms (tablets and creams). Under described conditions, CZE method is more selective, while the HPLC method is more sensitive. Both methods are rapid (t R (CZE)=5.14 min and t R (HPLC)=2.66 min), which is important for routine application. However, the HPLC method provides a repeatability of the quantitative analysis of ketoconazole in drug formulations below 1.5% relative standard deviation (R.S.D), while the repeatability of the CZE method is in the order of 2-3% R.S.D. PB - Elsevier Masson SAS T2 - Farmaco T1 - Comparison of capillary zone electrophoresis and high performance liquid chromatography methods for quantitative determination of ketoconazole in drug formulations VL - 59 IS - 5 SP - 419 EP - 424 DO - 10.1016/j.farmac.2003.11.019 ER -
@article{ author = "Velikinac, I and Čudina, Olivera and Janković, I and Agbaba, Danica and Vladimirov, S", year = "2004", abstract = "A capillary zone electrophoresis (CZE) and a high-performance liquid chromatography (HPLC) method have been developed for identification and determination of ketoconazole, an imidazole antifungal, in pharmaceutical preparations. The suitabilities of both methods for quantitative determination of ketoconazole were approved through validation specification such as linearity, precision, accuracy, limit of detection and quantification. The proposed methods were used for determination of ketoconazole in commercial pharmaceutical dosage forms (tablets and creams). Under described conditions, CZE method is more selective, while the HPLC method is more sensitive. Both methods are rapid (t R (CZE)=5.14 min and t R (HPLC)=2.66 min), which is important for routine application. However, the HPLC method provides a repeatability of the quantitative analysis of ketoconazole in drug formulations below 1.5% relative standard deviation (R.S.D), while the repeatability of the CZE method is in the order of 2-3% R.S.D.", publisher = "Elsevier Masson SAS", journal = "Farmaco", title = "Comparison of capillary zone electrophoresis and high performance liquid chromatography methods for quantitative determination of ketoconazole in drug formulations", volume = "59", number = "5", pages = "419-424", doi = "10.1016/j.farmac.2003.11.019" }
Velikinac, I., Čudina, O., Janković, I., Agbaba, D.,& Vladimirov, S.. (2004). Comparison of capillary zone electrophoresis and high performance liquid chromatography methods for quantitative determination of ketoconazole in drug formulations. in Farmaco Elsevier Masson SAS., 59(5), 419-424. https://doi.org/10.1016/j.farmac.2003.11.019
Velikinac I, Čudina O, Janković I, Agbaba D, Vladimirov S. Comparison of capillary zone electrophoresis and high performance liquid chromatography methods for quantitative determination of ketoconazole in drug formulations. in Farmaco. 2004;59(5):419-424. doi:10.1016/j.farmac.2003.11.019 .
Velikinac, I, Čudina, Olivera, Janković, I, Agbaba, Danica, Vladimirov, S, "Comparison of capillary zone electrophoresis and high performance liquid chromatography methods for quantitative determination of ketoconazole in drug formulations" in Farmaco, 59, no. 5 (2004):419-424, https://doi.org/10.1016/j.farmac.2003.11.019 . .