Investigation of chromatographic conditions for the separation of ofloxacin and its degradation products
Само за регистроване кориснике
2006
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A sensitive, precise, and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous determination of fluoroquinolone antibacterial ofloxacin and its degradation products: decarboxy ofloxacin, 9-piperazino ofloxacin, des-methyl ofloxacin, and ofloxacin-N-oxide. The proposed RP-HPLC method allowed separation of all five compounds simultaneously with the total time of the analysis not more than 15 min. The relative standard deviation (RSD) values for quantification of DOF, POF, MOF, OF, and NOF were of 0.77, 0.58, 0.51, 0.10, and 0.70%, respectively, indicating a good precision of the method. The limits of detection for DOF, POF, MOF, OF, and NOF were 0.10, 0.13, 0.06, 0.03, and 0.03 mu g mL(-1), respectively. The described method can be used for simultaneous identification and quantification of all analysed compounds in different pharmaceutical formulations.
Кључне речи:
liquid chromatography / ofloxacin / degradation products / determinationИзвор:
Journal of Chromatography A, 2006, 1119, 1-2, 224-230Издавач:
- Elsevier Science BV, Amsterdam
DOI: 10.1016/j.chroma.2006.02.029
ISSN: 0021-9673
PubMed: 16551467
WoS: 000238707100031
Scopus: 2-s2.0-33744792287
Институција/група
PharmacyTY - JOUR AU - Živanović, Lj. AU - Zigić, G. AU - Zečević, Mira PY - 2006 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/814 AB - A sensitive, precise, and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous determination of fluoroquinolone antibacterial ofloxacin and its degradation products: decarboxy ofloxacin, 9-piperazino ofloxacin, des-methyl ofloxacin, and ofloxacin-N-oxide. The proposed RP-HPLC method allowed separation of all five compounds simultaneously with the total time of the analysis not more than 15 min. The relative standard deviation (RSD) values for quantification of DOF, POF, MOF, OF, and NOF were of 0.77, 0.58, 0.51, 0.10, and 0.70%, respectively, indicating a good precision of the method. The limits of detection for DOF, POF, MOF, OF, and NOF were 0.10, 0.13, 0.06, 0.03, and 0.03 mu g mL(-1), respectively. The described method can be used for simultaneous identification and quantification of all analysed compounds in different pharmaceutical formulations. PB - Elsevier Science BV, Amsterdam T2 - Journal of Chromatography A T1 - Investigation of chromatographic conditions for the separation of ofloxacin and its degradation products VL - 1119 IS - 1-2 SP - 224 EP - 230 DO - 10.1016/j.chroma.2006.02.029 ER -
@article{ author = "Živanović, Lj. and Zigić, G. and Zečević, Mira", year = "2006", abstract = "A sensitive, precise, and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous determination of fluoroquinolone antibacterial ofloxacin and its degradation products: decarboxy ofloxacin, 9-piperazino ofloxacin, des-methyl ofloxacin, and ofloxacin-N-oxide. The proposed RP-HPLC method allowed separation of all five compounds simultaneously with the total time of the analysis not more than 15 min. The relative standard deviation (RSD) values for quantification of DOF, POF, MOF, OF, and NOF were of 0.77, 0.58, 0.51, 0.10, and 0.70%, respectively, indicating a good precision of the method. The limits of detection for DOF, POF, MOF, OF, and NOF were 0.10, 0.13, 0.06, 0.03, and 0.03 mu g mL(-1), respectively. The described method can be used for simultaneous identification and quantification of all analysed compounds in different pharmaceutical formulations.", publisher = "Elsevier Science BV, Amsterdam", journal = "Journal of Chromatography A", title = "Investigation of chromatographic conditions for the separation of ofloxacin and its degradation products", volume = "1119", number = "1-2", pages = "224-230", doi = "10.1016/j.chroma.2006.02.029" }
Živanović, Lj., Zigić, G.,& Zečević, M.. (2006). Investigation of chromatographic conditions for the separation of ofloxacin and its degradation products. in Journal of Chromatography A Elsevier Science BV, Amsterdam., 1119(1-2), 224-230. https://doi.org/10.1016/j.chroma.2006.02.029
Živanović L, Zigić G, Zečević M. Investigation of chromatographic conditions for the separation of ofloxacin and its degradation products. in Journal of Chromatography A. 2006;1119(1-2):224-230. doi:10.1016/j.chroma.2006.02.029 .
Živanović, Lj., Zigić, G., Zečević, Mira, "Investigation of chromatographic conditions for the separation of ofloxacin and its degradation products" in Journal of Chromatography A, 1119, no. 1-2 (2006):224-230, https://doi.org/10.1016/j.chroma.2006.02.029 . .