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High-performance liquid chromatographic method for the determination of moclobemide and its two major metabolites in human plasma
dc.creator | Rakić, Anita | |
dc.creator | Miljković, Branislava | |
dc.creator | Pokrajac, Milena | |
dc.creator | Vučićević, Katarina | |
dc.date.accessioned | 2019-09-02T11:10:14Z | |
dc.date.available | 2019-09-02T11:10:14Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 0731-7085 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/948 | |
dc.description.abstract | A selective, sensitive, and simple high-performance liquid chromatographic (HPLC) method was developed for the determination of moclobemide and its two major metabolites, Ro 12-5637 and Ro 12-8095, in human plasma. Sample preparation (0.5 ml of plasma) involved solid-phase extraction (SPE) using Speedisk (R) H2O-Philic DVB columns. Separations were performed on a Waters XTerra (TM) RP18 column (5 mu m, 150 mm x 4.6 mm). The mobile phase consisted of 10 mM KH2PO4 with 1% triethylamine (pH 3.9) and acetonitrile (93:17, v/v), and a flow-rate was 1.2 ml/min. The total run time was 13 min. UV detection was performed at 240 nm. Mean absolute recoveries were >= 90% and the limit of quantification (LOQ) for all analytes was 0.02 mg/l. Calibration curves were linear (r > 0.995) over a wide range of the analyte concentrations in plasma; thus, the method is suitable for different clinical studies when large variations in the drug/metabolites concentrations are observed. During a 5-day assay validation procedure the accuracy and precision were tested and proven (relative errors (RE) lt = 13%; intra-day coefficient of variation (CV) lt = 7%; inter-day CV lt = 13%). Many drugs frequently used in the target patient population were evaluated for potential interference in order method selectivity to be ensured. The assay has been used in a clinical pharmacokinetic study to assess steady-state pharmacokinetics of moclobenide and two metabolites in depressive patients on mono- and combined therapy. | en |
dc.publisher | Elsevier Science BV, Amsterdam | |
dc.rights | restrictedAccess | |
dc.source | Journal of Pharmaceutical and Biomedical Analysis | |
dc.subject | moclobemide | en |
dc.subject | metabolites | en |
dc.subject | plasma | en |
dc.subject | HPLC | en |
dc.subject | solid-phase extraction | en |
dc.title | High-performance liquid chromatographic method for the determination of moclobemide and its two major metabolites in human plasma | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Ракић, Aнита; Вучићевић, Катарина; Покрајац, Милена; Миљковић, Бранислава; | |
dc.citation.volume | 43 | |
dc.citation.issue | 4 | |
dc.citation.spage | 1416 | |
dc.citation.epage | 1422 | |
dc.citation.other | 43(4): 1416-1422 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000245262300031 | |
dc.identifier.doi | 10.1016/j.jpba.2006.10.032 | |
dc.identifier.pmid | 17141442 | |
dc.identifier.scopus | 2-s2.0-33847237604 | |
dc.type.version | publishedVersion |