TY  - JOUR
AU  - Dragičević, Nina
AU  - Krajišnik, Danina
AU  - Milić, Jela
AU  - Fahr, Alfred
AU  - Maibach, Howard
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3319
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3438
AB  - Objective: The aim of this study was to develop, characterize and evaluate stability of a gel containing coenzyme Q(10) (Q(10))-loaded liposomes, and enhance the stability of Q(10) in the nanocarrier-containing gel compared to the conventional gel. Methods: Q(10)-loaded liposome dispersions prepared from unsaturated or saturated lecithin, were characterized for particle size, polydispersity index (PDI), zeta-potential, pH value, oxidation index, Q(10)-content and morphology, and incorporated into carbomer gel. Liposome gels and liposome-free gel were analyzed for flow properties, pH values, Q(10)-content, and liposomes size and PDI (liposome gels), 48 h after preparation and in predetermined time intervals during 6 months storage at different temperatures in order to predict their long term stability. Results: Liposomes were of small particle size, homogeneous, negatively charged, and their incorporation into gel did not significantly change (p > .05) their particle size and PDI. All gels revealed non-Newtonian, shear-thinning plastic flow behavior during storage with no marked changes in rheological parameters. Storage of gels did not significantly influence the pH value (p > .05), while it significantly decreased Q(10)-content (p  lt  .05). Q(10) was significantly more (p  lt  .05) stable in liposome gel containing unsaturated lecithin liposomes (G1) than in gel containing saturated lecithin liposomes (G2) and liposome-free gel (G3). Conclusions: Q(10)-loaded liposome gel G1 was the optimal formulation, since during storage at different temperatures, it did not show significant increase in liposome size and PDI, it provided significantly higher stability for Q(10) than other gels and its pH value was suitable for skin application. Due to limited Q(10)-stability it should be stored at 4 degrees C.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Drug Development and Industrial Pharmacy
T1  - Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements
VL  - 45
IS  - 1
SP  - 43
EP  - 54
DO  - 10.1080/03639045.2018.1515220
ER  - 

@article{
author = "Dragičević, Nina and Krajišnik, Danina and Milić, Jela and Fahr, Alfred and Maibach, Howard",
year = "2019",
abstract = "Objective: The aim of this study was to develop, characterize and evaluate stability of a gel containing coenzyme Q(10) (Q(10))-loaded liposomes, and enhance the stability of Q(10) in the nanocarrier-containing gel compared to the conventional gel. Methods: Q(10)-loaded liposome dispersions prepared from unsaturated or saturated lecithin, were characterized for particle size, polydispersity index (PDI), zeta-potential, pH value, oxidation index, Q(10)-content and morphology, and incorporated into carbomer gel. Liposome gels and liposome-free gel were analyzed for flow properties, pH values, Q(10)-content, and liposomes size and PDI (liposome gels), 48 h after preparation and in predetermined time intervals during 6 months storage at different temperatures in order to predict their long term stability. Results: Liposomes were of small particle size, homogeneous, negatively charged, and their incorporation into gel did not significantly change (p > .05) their particle size and PDI. All gels revealed non-Newtonian, shear-thinning plastic flow behavior during storage with no marked changes in rheological parameters. Storage of gels did not significantly influence the pH value (p > .05), while it significantly decreased Q(10)-content (p  lt  .05). Q(10) was significantly more (p  lt  .05) stable in liposome gel containing unsaturated lecithin liposomes (G1) than in gel containing saturated lecithin liposomes (G2) and liposome-free gel (G3). Conclusions: Q(10)-loaded liposome gel G1 was the optimal formulation, since during storage at different temperatures, it did not show significant increase in liposome size and PDI, it provided significantly higher stability for Q(10) than other gels and its pH value was suitable for skin application. Due to limited Q(10)-stability it should be stored at 4 degrees C.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Drug Development and Industrial Pharmacy",
title = "Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements",
volume = "45",
number = "1",
pages = "43-54",
doi = "10.1080/03639045.2018.1515220"
}

Dragičević, N., Krajišnik, D., Milić, J., Fahr, A.,& Maibach, H.. (2019). Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements. in Drug Development and Industrial Pharmacy
Taylor & Francis Ltd, Abingdon., 45(1), 43-54.
https://doi.org/10.1080/03639045.2018.1515220

Dragičević N, Krajišnik D, Milić J, Fahr A, Maibach H. Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements. in Drug Development and Industrial Pharmacy. 2019;45(1):43-54.
doi:10.1080/03639045.2018.1515220 .

Dragičević, Nina, Krajišnik, Danina, Milić, Jela, Fahr, Alfred, Maibach, Howard, "Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements" in Drug Development and Industrial Pharmacy, 45, no. 1 (2019):43-54,
https://doi.org/10.1080/03639045.2018.1515220 . .

TY  - JOUR
AU  - Dragičević, Nina
AU  - Krajišnik, Danina
AU  - Milić, Jela
AU  - Fahr, Alfred
AU  - Maibach, Howard
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3319
AB  - Objective: The aim of this study was to develop, characterize and evaluate stability of a gel containing coenzyme Q(10) (Q(10))-loaded liposomes, and enhance the stability of Q(10) in the nanocarrier-containing gel compared to the conventional gel. Methods: Q(10)-loaded liposome dispersions prepared from unsaturated or saturated lecithin, were characterized for particle size, polydispersity index (PDI), zeta-potential, pH value, oxidation index, Q(10)-content and morphology, and incorporated into carbomer gel. Liposome gels and liposome-free gel were analyzed for flow properties, pH values, Q(10)-content, and liposomes size and PDI (liposome gels), 48 h after preparation and in predetermined time intervals during 6 months storage at different temperatures in order to predict their long term stability. Results: Liposomes were of small particle size, homogeneous, negatively charged, and their incorporation into gel did not significantly change (p > .05) their particle size and PDI. All gels revealed non-Newtonian, shear-thinning plastic flow behavior during storage with no marked changes in rheological parameters. Storage of gels did not significantly influence the pH value (p > .05), while it significantly decreased Q(10)-content (p  lt  .05). Q(10) was significantly more (p  lt  .05) stable in liposome gel containing unsaturated lecithin liposomes (G1) than in gel containing saturated lecithin liposomes (G2) and liposome-free gel (G3). Conclusions: Q(10)-loaded liposome gel G1 was the optimal formulation, since during storage at different temperatures, it did not show significant increase in liposome size and PDI, it provided significantly higher stability for Q(10) than other gels and its pH value was suitable for skin application. Due to limited Q(10)-stability it should be stored at 4 degrees C.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Drug Development and Industrial Pharmacy
T1  - Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements
VL  - 45
IS  - 1
SP  - 43
EP  - 54
DO  - 10.1080/03639045.2018.1515220
ER  - 

@article{
author = "Dragičević, Nina and Krajišnik, Danina and Milić, Jela and Fahr, Alfred and Maibach, Howard",
year = "2019",
abstract = "Objective: The aim of this study was to develop, characterize and evaluate stability of a gel containing coenzyme Q(10) (Q(10))-loaded liposomes, and enhance the stability of Q(10) in the nanocarrier-containing gel compared to the conventional gel. Methods: Q(10)-loaded liposome dispersions prepared from unsaturated or saturated lecithin, were characterized for particle size, polydispersity index (PDI), zeta-potential, pH value, oxidation index, Q(10)-content and morphology, and incorporated into carbomer gel. Liposome gels and liposome-free gel were analyzed for flow properties, pH values, Q(10)-content, and liposomes size and PDI (liposome gels), 48 h after preparation and in predetermined time intervals during 6 months storage at different temperatures in order to predict their long term stability. Results: Liposomes were of small particle size, homogeneous, negatively charged, and their incorporation into gel did not significantly change (p > .05) their particle size and PDI. All gels revealed non-Newtonian, shear-thinning plastic flow behavior during storage with no marked changes in rheological parameters. Storage of gels did not significantly influence the pH value (p > .05), while it significantly decreased Q(10)-content (p  lt  .05). Q(10) was significantly more (p  lt  .05) stable in liposome gel containing unsaturated lecithin liposomes (G1) than in gel containing saturated lecithin liposomes (G2) and liposome-free gel (G3). Conclusions: Q(10)-loaded liposome gel G1 was the optimal formulation, since during storage at different temperatures, it did not show significant increase in liposome size and PDI, it provided significantly higher stability for Q(10) than other gels and its pH value was suitable for skin application. Due to limited Q(10)-stability it should be stored at 4 degrees C.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Drug Development and Industrial Pharmacy",
title = "Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements",
volume = "45",
number = "1",
pages = "43-54",
doi = "10.1080/03639045.2018.1515220"
}

Dragičević, N., Krajišnik, D., Milić, J., Fahr, A.,& Maibach, H.. (2019). Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements. in Drug Development and Industrial Pharmacy
Taylor & Francis Ltd, Abingdon., 45(1), 43-54.
https://doi.org/10.1080/03639045.2018.1515220

Dragičević N, Krajišnik D, Milić J, Fahr A, Maibach H. Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements. in Drug Development and Industrial Pharmacy. 2019;45(1):43-54.
doi:10.1080/03639045.2018.1515220 .

Dragičević, Nina, Krajišnik, Danina, Milić, Jela, Fahr, Alfred, Maibach, Howard, "Development of hydrophilic gels containing coenzyme Q(10)-loaded liposomes: characterization, stability and rheology measurements" in Drug Development and Industrial Pharmacy, 45, no. 1 (2019):43-54,
https://doi.org/10.1080/03639045.2018.1515220 . .

TY  - JOUR
AU  - Dragičević-Curić, Nina
AU  - Winter, Sven
AU  - Krajišnik, Danina
AU  - Stupar, Mirjana
AU  - Milić, Jela
AU  - Graefe, Susanna
AU  - Fahr, Alfred
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1386
AB  - Temoporfin (mTHPC) is a potent second-generation synthetic photosensitizer. Topical delivery of mTHPC is of great interest for the photodynamic therapy of psoriasis and superficial skin cancer lesions. The aim of this study was to evaluate the stability of hydrophilic gels containing mTHPC-loaded liposomes. Two different mTHPC-loaded liposome dispersions, composed of 15 % (w/w) nonhydrogenated soybean lecithin of different phosphatidylcholine content, were prepared and incorporated (2:1 w/w) into hydrogels of different carbomer concentrations (1.5, 2.25, and 3%; w/w). Obtained liposomal hydrogels, containing 0.15% (w/w) mTHPC, 10% (w/w) phospholipids, and 0, 0.5, or 1% (w/w) carbomer, were analyzed for flow properties, liposome particle size, and polydispersity index (PDI), pH value, and mTHPC content after their preparation and at predetermined time intervals during 6 months of storage at 4 and 23 degrees C. All hydrogels showed, during the whole period of investigation, adequate characteristics for topical application (i.e., they revealed shear-thinning plastic flow behavior). Rheological parameters, particle size, and PDI of liposomes in hydrogels, mTHPC content, and pH value did not show remarkable changes during the storage of gels, which could make them unacceptable for topical use. The obtained results indicated physical and chemical stability of liposomal gels containing mTHPC during 6 months of storage at both temperatures.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Liposome Research
T1  - Stability evaluation of temoporfin-loaded liposomal gels for topical application
VL  - 20
IS  - 1
SP  - 38
EP  - 48
DO  - 10.3109/08982100903030263
ER  - 

@article{
author = "Dragičević-Curić, Nina and Winter, Sven and Krajišnik, Danina and Stupar, Mirjana and Milić, Jela and Graefe, Susanna and Fahr, Alfred",
year = "2010",
abstract = "Temoporfin (mTHPC) is a potent second-generation synthetic photosensitizer. Topical delivery of mTHPC is of great interest for the photodynamic therapy of psoriasis and superficial skin cancer lesions. The aim of this study was to evaluate the stability of hydrophilic gels containing mTHPC-loaded liposomes. Two different mTHPC-loaded liposome dispersions, composed of 15 % (w/w) nonhydrogenated soybean lecithin of different phosphatidylcholine content, were prepared and incorporated (2:1 w/w) into hydrogels of different carbomer concentrations (1.5, 2.25, and 3%; w/w). Obtained liposomal hydrogels, containing 0.15% (w/w) mTHPC, 10% (w/w) phospholipids, and 0, 0.5, or 1% (w/w) carbomer, were analyzed for flow properties, liposome particle size, and polydispersity index (PDI), pH value, and mTHPC content after their preparation and at predetermined time intervals during 6 months of storage at 4 and 23 degrees C. All hydrogels showed, during the whole period of investigation, adequate characteristics for topical application (i.e., they revealed shear-thinning plastic flow behavior). Rheological parameters, particle size, and PDI of liposomes in hydrogels, mTHPC content, and pH value did not show remarkable changes during the storage of gels, which could make them unacceptable for topical use. The obtained results indicated physical and chemical stability of liposomal gels containing mTHPC during 6 months of storage at both temperatures.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Liposome Research",
title = "Stability evaluation of temoporfin-loaded liposomal gels for topical application",
volume = "20",
number = "1",
pages = "38-48",
doi = "10.3109/08982100903030263"
}

Dragičević-Curić, N., Winter, S., Krajišnik, D., Stupar, M., Milić, J., Graefe, S.,& Fahr, A.. (2010). Stability evaluation of temoporfin-loaded liposomal gels for topical application. in Journal of Liposome Research
Taylor & Francis Ltd, Abingdon., 20(1), 38-48.
https://doi.org/10.3109/08982100903030263

Dragičević-Curić N, Winter S, Krajišnik D, Stupar M, Milić J, Graefe S, Fahr A. Stability evaluation of temoporfin-loaded liposomal gels for topical application. in Journal of Liposome Research. 2010;20(1):38-48.
doi:10.3109/08982100903030263 .

Dragičević-Curić, Nina, Winter, Sven, Krajišnik, Danina, Stupar, Mirjana, Milić, Jela, Graefe, Susanna, Fahr, Alfred, "Stability evaluation of temoporfin-loaded liposomal gels for topical application" in Journal of Liposome Research, 20, no. 1 (2010):38-48,
https://doi.org/10.3109/08982100903030263 . .

TY  - JOUR
AU  - Dragičević-Curić, Nina
AU  - Winter, Sven
AU  - Stupar, Mirjana
AU  - Milić, Jela
AU  - Krajišnik, Danina
AU  - Gitter, Burkhard
AU  - Fahr, Alfred
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1231
AB  - Temoporfin (mTHPC) is a potent second-generation photosensitizer. The primary object of this study was to develop a topical mTHPC-loaded liposomal hydrogel able to deliver mTHPC into the stratum corneum (SC) and deeper skin layers. This study was conducted (1) to determine the effect of carbomer concentration, used as a gelling agent, and the effect of phosphatidylcholine (PC) content of lecithin, used for the liposome preparation. on viscoelastic properties and viscosity of liposomal gels and (2) to determine the relationship between theological properties of gels and the skin penetration of mTHPC. Liposomal hydrogels revealed plastic flow behaviour. The increase of carbomer concentration induced a domination of elastic over viscous behaviour of gels. There was an inverse relationship between the elasticity of gels and mTHPC-penetration. Viscosity also increased with the increment of carbomer concentration, reducing the mTHPC-penetration. Liposomal gels containing lecithin of smaller PC-content (i.e. smaller purity) exhibited a more elastic solid behaviour than gels containing lecithin with high PC-content, and showed smaller mTHPC-penetration. The gel containing 0.75%, w/w, carbomer and lecithin with high PC-content was considered to be the optimal formulation, since it delivered high amounts of mTHPC to the SC and deeper skin layers, and it possessed desirable theological properties.
PB  - Elsevier Science BV, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Temoporfin-loaded liposomal gels: Viscoelastic properties and in vitro skin penetration
VL  - 373
IS  - 1-2
SP  - 77
EP  - 84
DO  - 10.1016/j.ijpharm.2009.02.010
ER  - 

@article{
author = "Dragičević-Curić, Nina and Winter, Sven and Stupar, Mirjana and Milić, Jela and Krajišnik, Danina and Gitter, Burkhard and Fahr, Alfred",
year = "2009",
abstract = "Temoporfin (mTHPC) is a potent second-generation photosensitizer. The primary object of this study was to develop a topical mTHPC-loaded liposomal hydrogel able to deliver mTHPC into the stratum corneum (SC) and deeper skin layers. This study was conducted (1) to determine the effect of carbomer concentration, used as a gelling agent, and the effect of phosphatidylcholine (PC) content of lecithin, used for the liposome preparation. on viscoelastic properties and viscosity of liposomal gels and (2) to determine the relationship between theological properties of gels and the skin penetration of mTHPC. Liposomal hydrogels revealed plastic flow behaviour. The increase of carbomer concentration induced a domination of elastic over viscous behaviour of gels. There was an inverse relationship between the elasticity of gels and mTHPC-penetration. Viscosity also increased with the increment of carbomer concentration, reducing the mTHPC-penetration. Liposomal gels containing lecithin of smaller PC-content (i.e. smaller purity) exhibited a more elastic solid behaviour than gels containing lecithin with high PC-content, and showed smaller mTHPC-penetration. The gel containing 0.75%, w/w, carbomer and lecithin with high PC-content was considered to be the optimal formulation, since it delivered high amounts of mTHPC to the SC and deeper skin layers, and it possessed desirable theological properties.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Temoporfin-loaded liposomal gels: Viscoelastic properties and in vitro skin penetration",
volume = "373",
number = "1-2",
pages = "77-84",
doi = "10.1016/j.ijpharm.2009.02.010"
}

Dragičević-Curić, N., Winter, S., Stupar, M., Milić, J., Krajišnik, D., Gitter, B.,& Fahr, A.. (2009). Temoporfin-loaded liposomal gels: Viscoelastic properties and in vitro skin penetration. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 373(1-2), 77-84.
https://doi.org/10.1016/j.ijpharm.2009.02.010

Dragičević-Curić N, Winter S, Stupar M, Milić J, Krajišnik D, Gitter B, Fahr A. Temoporfin-loaded liposomal gels: Viscoelastic properties and in vitro skin penetration. in International Journal of Pharmaceutics. 2009;373(1-2):77-84.
doi:10.1016/j.ijpharm.2009.02.010 .

Dragičević-Curić, Nina, Winter, Sven, Stupar, Mirjana, Milić, Jela, Krajišnik, Danina, Gitter, Burkhard, Fahr, Alfred, "Temoporfin-loaded liposomal gels: Viscoelastic properties and in vitro skin penetration" in International Journal of Pharmaceutics, 373, no. 1-2 (2009):77-84,
https://doi.org/10.1016/j.ijpharm.2009.02.010 . .