TY  - JOUR
AU  - Filipović, Nenad
AU  - Veselinovic, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipić, Metka
AU  - Zegura, Bojana
AU  - Tomic, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3328
AB  - Poly (e-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1-4 mu m with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants.
PB  - Elsevier Science BV, Amsterdam
T2  - Materials Science & Engineering C: Materials for Biological Applications
T1  - Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles
VL  - 96
SP  - 776
EP  - 789
DO  - 10.1016/j.msec.2018.11.073
ER  - 

@article{
author = "Filipović, Nenad and Veselinovic, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipić, Metka and Zegura, Bojana and Tomic, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
abstract = "Poly (e-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1-4 mu m with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Materials Science & Engineering C: Materials for Biological Applications",
title = "Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles",
volume = "96",
pages = "776-789",
doi = "10.1016/j.msec.2018.11.073"
}

Filipović, N., Veselinovic, L., Ražić, S., Jeremić, S., Filipić, M., Zegura, B., Tomic, S., Čolić, M.,& Stevanović, M.. (2019). Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science & Engineering C: Materials for Biological Applications
Elsevier Science BV, Amsterdam., 96, 776-789.
https://doi.org/10.1016/j.msec.2018.11.073

Filipović N, Veselinovic L, Ražić S, Jeremić S, Filipić M, Zegura B, Tomic S, Čolić M, Stevanović M. Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science & Engineering C: Materials for Biological Applications. 2019;96:776-789.
doi:10.1016/j.msec.2018.11.073 .

Filipović, Nenad, Veselinovic, Ljiljana, Ražić, Slavica, Jeremić, Sanja, Filipić, Metka, Zegura, Bojana, Tomic, Sergej, Čolić, Miodrag, Stevanović, Magdalena, "Poly (epsilon-caprolactone) microspheres for prolonged release of selenium nanoparticles" in Materials Science & Engineering C: Materials for Biological Applications, 96 (2019):776-789,
https://doi.org/10.1016/j.msec.2018.11.073 . .

TY  - JOUR
AU  - Stojić-Vukanić, Zorica
AU  - Čolić, Miodrag
AU  - Backović, A.
AU  - Antić-Stanković, Jelena
AU  - Bufan, Biljana
AU  - Dimitrijević, M.
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1580
AB  - Leflunomide is an immunosuppressive drug effective in experimental models of transplantation and autoimmune diseases and in the treatment of active rheumatoid arthritis (RA). Having in mind that it has been shown that some other immunosuppressive drugs (glucocorticoids, mycophenolate mofetil, sirolimus etc.) impair dendritic cell (DC) phenotype and function, we investigated the effect of A77 1726, an active metabolite of leflunomide, on the differentiation and function of human monocyte-derived dendritic cells (MDDC) in vitro. Immature MDDC were generated by cultivating monocytes in medium supplemented with GM-CSF and IL-4. To induce maturation, immature MDDC were cultured for 2 additional days with LPS. A77 1726 (100 μM) was added at the beginning of cultivation. Flow cytometric analysis showed that MDDC differentiated in the presence of A77 1726 exhibited an altered phenotype, with a down-regulated surface expression of CD80, CD86, CD54 and CD40 molecules. Furthermore, the continuous presence of A77 1726 during differentiation and maturation prevented successful maturation, judging by the decreased expression of maturation marker CD83, costimulatory and adhesive molecules on A77 1726-treated mature MDDC. In addition, A77 1726-pretreated MDDC exhibited a poor stimulatory capacity of the allogeneic T cells and a low production of IL-10 and IL-18. These data suggest that leflunomide impairs the differentiation, maturation and function of human MDDC in vitro, which is an additional mechanism of its immunosuppressive effect.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Differentiation and function of human monocyte-derived dendritic cells under the influence of leflunomide
VL  - 63
IS  - 2
SP  - 353
EP  - 364
DO  - 10.2298/ABS1102353S
ER  - 

@article{
author = "Stojić-Vukanić, Zorica and Čolić, Miodrag and Backović, A. and Antić-Stanković, Jelena and Bufan, Biljana and Dimitrijević, M.",
year = "2011",
abstract = "Leflunomide is an immunosuppressive drug effective in experimental models of transplantation and autoimmune diseases and in the treatment of active rheumatoid arthritis (RA). Having in mind that it has been shown that some other immunosuppressive drugs (glucocorticoids, mycophenolate mofetil, sirolimus etc.) impair dendritic cell (DC) phenotype and function, we investigated the effect of A77 1726, an active metabolite of leflunomide, on the differentiation and function of human monocyte-derived dendritic cells (MDDC) in vitro. Immature MDDC were generated by cultivating monocytes in medium supplemented with GM-CSF and IL-4. To induce maturation, immature MDDC were cultured for 2 additional days with LPS. A77 1726 (100 μM) was added at the beginning of cultivation. Flow cytometric analysis showed that MDDC differentiated in the presence of A77 1726 exhibited an altered phenotype, with a down-regulated surface expression of CD80, CD86, CD54 and CD40 molecules. Furthermore, the continuous presence of A77 1726 during differentiation and maturation prevented successful maturation, judging by the decreased expression of maturation marker CD83, costimulatory and adhesive molecules on A77 1726-treated mature MDDC. In addition, A77 1726-pretreated MDDC exhibited a poor stimulatory capacity of the allogeneic T cells and a low production of IL-10 and IL-18. These data suggest that leflunomide impairs the differentiation, maturation and function of human MDDC in vitro, which is an additional mechanism of its immunosuppressive effect.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Differentiation and function of human monocyte-derived dendritic cells under the influence of leflunomide",
volume = "63",
number = "2",
pages = "353-364",
doi = "10.2298/ABS1102353S"
}

Stojić-Vukanić, Z., Čolić, M., Backović, A., Antić-Stanković, J., Bufan, B.,& Dimitrijević, M.. (2011). Differentiation and function of human monocyte-derived dendritic cells under the influence of leflunomide. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(2), 353-364.
https://doi.org/10.2298/ABS1102353S

Stojić-Vukanić Z, Čolić M, Backović A, Antić-Stanković J, Bufan B, Dimitrijević M. Differentiation and function of human monocyte-derived dendritic cells under the influence of leflunomide. in Archives of Biological Sciences. 2011;63(2):353-364.
doi:10.2298/ABS1102353S .

Stojić-Vukanić, Zorica, Čolić, Miodrag, Backović, A., Antić-Stanković, Jelena, Bufan, Biljana, Dimitrijević, M., "Differentiation and function of human monocyte-derived dendritic cells under the influence of leflunomide" in Archives of Biological Sciences, 63, no. 2 (2011):353-364,
https://doi.org/10.2298/ABS1102353S . .

TY  - JOUR
AU  - Bufan, Biljana
AU  - Mojsilović, Slavko
AU  - Božić, Dragana
AU  - Vucević, Dragana
AU  - Vasilijić, Saša
AU  - Balint, Bela
AU  - Čolić, Miodrag
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1276
AB  - Acetylsalicilyc acid (aspirin, ASA) is a well known anti-inflammatory drug with immunomodulatory properties. NO-releasing aspirins (NO-ASA) are new compounds with anti-inflammatory properties. We studied the effects of ASA and two NO-ASA (NCX 4016 and NCX 4040) on human monocyte-derived dendritic cells (MoDC). Immature MoDC were generated in vitro from monocytes in the presence of recombinant human granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4. Mature MoDC were obtained by adding lipopolysaccharide (LPS) in cultures of immature MoDC. As we found that ASA at 4-8 mM, NCX 4016 at 400-800 mu M and NCX 4040 at 4-8 mu M stimulated apoptosis of monocytes and immature MoDC, sub-apoptotic concentrations of ASA (2 mM), NCX 4016 (200 mu M) and NCX 4040 (2 mu M) were used in experiments. Examined substances were added at the beginning of MoDC cultivation. MoDC differentiated in the presence of examined compounds had lower expression of HLA-DR, CD80, CD40 and CD54, decreased allostimulatory activity and lower production of IL-12 p40. ASA and NCX 4016 decreased production of IL-10, whereas NCX 4040 had the opposite effect. ASA inhibited the expression of CD1a and prevented downregulation of 014, NCX 4016 stimulated the differentiation of CD1a(+)CD14(+) and CD1a(-)CD14(+) cells, whereas NCX 4040 decreased the proportion of CD1a(+)CD14(-) and increased the frequency of CD1a(+)CD14(+) cells, compared to control. Maturation, both in ASA and NO-ASA treated MoDC was characterized by decreased allostimulatory activity, lower expression of CD83, HLA-DR, costimulatory molecules and CD54 and decreased production of IL-10 and IL-12 p40. In conclusion, we confirmed that ASA impairs differentiation, maturation and function of MoDC and found that NCX 4016 and NCX 4040 exerted similar, but not identical effects at about 10- and 1000-fold lower concentrations, respectively, compared to ASA.
PB  - Elsevier Science BV, Amsterdam
T2  - International Immunopharmacology
T1  - Comparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitro
VL  - 9
IS  - 7-8
SP  - 910
EP  - 917
DO  - 10.1016/j.intimp.2009.03.016
ER  - 

@article{
author = "Bufan, Biljana and Mojsilović, Slavko and Božić, Dragana and Vucević, Dragana and Vasilijić, Saša and Balint, Bela and Čolić, Miodrag",
year = "2009",
abstract = "Acetylsalicilyc acid (aspirin, ASA) is a well known anti-inflammatory drug with immunomodulatory properties. NO-releasing aspirins (NO-ASA) are new compounds with anti-inflammatory properties. We studied the effects of ASA and two NO-ASA (NCX 4016 and NCX 4040) on human monocyte-derived dendritic cells (MoDC). Immature MoDC were generated in vitro from monocytes in the presence of recombinant human granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4. Mature MoDC were obtained by adding lipopolysaccharide (LPS) in cultures of immature MoDC. As we found that ASA at 4-8 mM, NCX 4016 at 400-800 mu M and NCX 4040 at 4-8 mu M stimulated apoptosis of monocytes and immature MoDC, sub-apoptotic concentrations of ASA (2 mM), NCX 4016 (200 mu M) and NCX 4040 (2 mu M) were used in experiments. Examined substances were added at the beginning of MoDC cultivation. MoDC differentiated in the presence of examined compounds had lower expression of HLA-DR, CD80, CD40 and CD54, decreased allostimulatory activity and lower production of IL-12 p40. ASA and NCX 4016 decreased production of IL-10, whereas NCX 4040 had the opposite effect. ASA inhibited the expression of CD1a and prevented downregulation of 014, NCX 4016 stimulated the differentiation of CD1a(+)CD14(+) and CD1a(-)CD14(+) cells, whereas NCX 4040 decreased the proportion of CD1a(+)CD14(-) and increased the frequency of CD1a(+)CD14(+) cells, compared to control. Maturation, both in ASA and NO-ASA treated MoDC was characterized by decreased allostimulatory activity, lower expression of CD83, HLA-DR, costimulatory molecules and CD54 and decreased production of IL-10 and IL-12 p40. In conclusion, we confirmed that ASA impairs differentiation, maturation and function of MoDC and found that NCX 4016 and NCX 4040 exerted similar, but not identical effects at about 10- and 1000-fold lower concentrations, respectively, compared to ASA.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Immunopharmacology",
title = "Comparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitro",
volume = "9",
number = "7-8",
pages = "910-917",
doi = "10.1016/j.intimp.2009.03.016"
}

Bufan, B., Mojsilović, S., Božić, D., Vucević, D., Vasilijić, S., Balint, B.,& Čolić, M.. (2009). Comparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitro. in International Immunopharmacology
Elsevier Science BV, Amsterdam., 9(7-8), 910-917.
https://doi.org/10.1016/j.intimp.2009.03.016

Bufan B, Mojsilović S, Božić D, Vucević D, Vasilijić S, Balint B, Čolić M. Comparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitro. in International Immunopharmacology. 2009;9(7-8):910-917.
doi:10.1016/j.intimp.2009.03.016 .

Bufan, Biljana, Mojsilović, Slavko, Božić, Dragana, Vucević, Dragana, Vasilijić, Saša, Balint, Bela, Čolić, Miodrag, "Comparative effects of aspirin and NO-releasing aspirins on differentiation, maturation and function of human monocyte-derived dendritic cells in vitro" in International Immunopharmacology, 9, no. 7-8 (2009):910-917,
https://doi.org/10.1016/j.intimp.2009.03.016 . .

TY  - JOUR
AU  - Stevanović, Nana D.
AU  - Jovanović, Marina
AU  - Jelenković, Ankica V.
AU  - Ninković, Milica
AU  - Đukić, Mirjana
AU  - Stojanović, Ivana
AU  - Čolić, Miodrag
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1182
AB  - The goal of the present study was to examine the effectiveness of a non-specific nitric oxide synthase (NOS) inhibitor N-nitro-L-arginine methyl ester (L-NAME) to modulate the toxicity of aluminium chloride (AlCl3). Rats were killed at 3 h and at 30 days after treatments and the striatum was removed. Nitrite, superoxide, superoxide dismutase activity, malondialdehyde and reduced glutathione were determined. AlCl3 exposure promoted oxidative stress in the striatum. The biochemical changes observed in neuronal tissues show that aluminium acts as pro-oxidant, while the NOS inhibitor exerts antioxidant action in AlCl3-treated rats. We conclude that L-NAME can efficiently protect neuronal tissue from AlCl3-induced toxicity.
PB  - General Physiol And Biophysics, Bratislava
T2  - General Physiology and Biophysics
T1  - The effect of inhibition of nitric oxide synthase on aluminium-induced toxicity in the rat brain
VL  - 28
SP  - 235
EP  - 242
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1182
ER  - 

@article{
author = "Stevanović, Nana D. and Jovanović, Marina and Jelenković, Ankica V. and Ninković, Milica and Đukić, Mirjana and Stojanović, Ivana and Čolić, Miodrag",
year = "2009",
abstract = "The goal of the present study was to examine the effectiveness of a non-specific nitric oxide synthase (NOS) inhibitor N-nitro-L-arginine methyl ester (L-NAME) to modulate the toxicity of aluminium chloride (AlCl3). Rats were killed at 3 h and at 30 days after treatments and the striatum was removed. Nitrite, superoxide, superoxide dismutase activity, malondialdehyde and reduced glutathione were determined. AlCl3 exposure promoted oxidative stress in the striatum. The biochemical changes observed in neuronal tissues show that aluminium acts as pro-oxidant, while the NOS inhibitor exerts antioxidant action in AlCl3-treated rats. We conclude that L-NAME can efficiently protect neuronal tissue from AlCl3-induced toxicity.",
publisher = "General Physiol And Biophysics, Bratislava",
journal = "General Physiology and Biophysics",
title = "The effect of inhibition of nitric oxide synthase on aluminium-induced toxicity in the rat brain",
volume = "28",
pages = "235-242",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1182"
}

Stevanović, N. D., Jovanović, M., Jelenković, A. V., Ninković, M., Đukić, M., Stojanović, I.,& Čolić, M.. (2009). The effect of inhibition of nitric oxide synthase on aluminium-induced toxicity in the rat brain. in General Physiology and Biophysics
General Physiol And Biophysics, Bratislava., 28, 235-242.
https://hdl.handle.net/21.15107/rcub_farfar_1182

Stevanović ND, Jovanović M, Jelenković AV, Ninković M, Đukić M, Stojanović I, Čolić M. The effect of inhibition of nitric oxide synthase on aluminium-induced toxicity in the rat brain. in General Physiology and Biophysics. 2009;28:235-242.
https://hdl.handle.net/21.15107/rcub_farfar_1182 .

Stevanović, Nana D., Jovanović, Marina, Jelenković, Ankica V., Ninković, Milica, Đukić, Mirjana, Stojanović, Ivana, Čolić, Miodrag, "The effect of inhibition of nitric oxide synthase on aluminium-induced toxicity in the rat brain" in General Physiology and Biophysics, 28 (2009):235-242,
https://hdl.handle.net/21.15107/rcub_farfar_1182 .

TY  - JOUR
AU  - Pilipović, Ivan
AU  - Vidić-Danković, Biljana
AU  - Perišić, Milica
AU  - Radojević, Katarina
AU  - Čolić, Miodrag
AU  - Todorović, Vera
AU  - Leposavić, Gordana
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1080
AB  - The study was undertaken to explore whether there were: i) apart from neural and circulatory, some other sources of catecholamines (CAs) in rat thymus and ii) gender-specific differences in thymic CA levels, and if so to elucidate the role of sex steroids in this phenomenon. Tyrosine hydroxylase (TH) immunoreactivity was found in thymocytes and thymic epithelial cells (some of which showed morphological features of nurse cells). The density of CA-synthesizing cells was greater in male than in female rats. Noradrenaline (NA), but not dopamine (DA), was detected in thymocytes. NA and DA levels in thymi, and the NA level in thymocytes, were higher in male rats. To explore the Putative role of sex steroids in this dichotomy in the thymi of adult rats gonadectomized (Gx) or sham-Gx at the age of 30 days the density of TH+ cells and CA levels were measured. Gonadectomy abolished sexual dimorphism in the density of thymic TH+ cells (diminishing their density in male rats) and thymic CA levels (the NA levels were reduced in rats of both sexes and also the DA level in male rats). Therefore, it can be assumed that testicular and ovarian hormones control thymic NA and DA levels via different mechanisms. Moreover, in Gx rats, despite the decrease in the overall thymic NA level, an increase in the thymocyte NA level was found indicating that gonadal hormones exert differential effects on the NA level in distinct thymic cellular compartments.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Neuroimmunology
T1  - Sexual dimorphism in the catecholamine-containing thymus microenvironment: A role for gonadal hormones
VL  - 195
IS  - 1-2
SP  - 7
EP  - 20
DO  - 10.1016/j.jneuroim.2007.12.006
ER  - 

@article{
author = "Pilipović, Ivan and Vidić-Danković, Biljana and Perišić, Milica and Radojević, Katarina and Čolić, Miodrag and Todorović, Vera and Leposavić, Gordana",
year = "2008",
abstract = "The study was undertaken to explore whether there were: i) apart from neural and circulatory, some other sources of catecholamines (CAs) in rat thymus and ii) gender-specific differences in thymic CA levels, and if so to elucidate the role of sex steroids in this phenomenon. Tyrosine hydroxylase (TH) immunoreactivity was found in thymocytes and thymic epithelial cells (some of which showed morphological features of nurse cells). The density of CA-synthesizing cells was greater in male than in female rats. Noradrenaline (NA), but not dopamine (DA), was detected in thymocytes. NA and DA levels in thymi, and the NA level in thymocytes, were higher in male rats. To explore the Putative role of sex steroids in this dichotomy in the thymi of adult rats gonadectomized (Gx) or sham-Gx at the age of 30 days the density of TH+ cells and CA levels were measured. Gonadectomy abolished sexual dimorphism in the density of thymic TH+ cells (diminishing their density in male rats) and thymic CA levels (the NA levels were reduced in rats of both sexes and also the DA level in male rats). Therefore, it can be assumed that testicular and ovarian hormones control thymic NA and DA levels via different mechanisms. Moreover, in Gx rats, despite the decrease in the overall thymic NA level, an increase in the thymocyte NA level was found indicating that gonadal hormones exert differential effects on the NA level in distinct thymic cellular compartments.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Sexual dimorphism in the catecholamine-containing thymus microenvironment: A role for gonadal hormones",
volume = "195",
number = "1-2",
pages = "7-20",
doi = "10.1016/j.jneuroim.2007.12.006"
}

Pilipović, I., Vidić-Danković, B., Perišić, M., Radojević, K., Čolić, M., Todorović, V.,& Leposavić, G.. (2008). Sexual dimorphism in the catecholamine-containing thymus microenvironment: A role for gonadal hormones. in Journal of Neuroimmunology
Elsevier Science BV, Amsterdam., 195(1-2), 7-20.
https://doi.org/10.1016/j.jneuroim.2007.12.006

Pilipović I, Vidić-Danković B, Perišić M, Radojević K, Čolić M, Todorović V, Leposavić G. Sexual dimorphism in the catecholamine-containing thymus microenvironment: A role for gonadal hormones. in Journal of Neuroimmunology. 2008;195(1-2):7-20.
doi:10.1016/j.jneuroim.2007.12.006 .

Pilipović, Ivan, Vidić-Danković, Biljana, Perišić, Milica, Radojević, Katarina, Čolić, Miodrag, Todorović, Vera, Leposavić, Gordana, "Sexual dimorphism in the catecholamine-containing thymus microenvironment: A role for gonadal hormones" in Journal of Neuroimmunology, 195, no. 1-2 (2008):7-20,
https://doi.org/10.1016/j.jneuroim.2007.12.006 . .

TY  - JOUR
AU  - Stojić-Vukanić, Zorica
AU  - Čolić, Miodrag
AU  - Dimitrijević, M.
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/917
AB  - Pentoxifylline (PTX) is a drug used for the treatment of vascular disorders, but it also has a positive therapeutic effect in experimental models of some autoimmune diseases. In this work, we studied the effect of PTX on human monocyte-derived dendritic cells (MDDCs). Immature MDDCs were generated in vitro from monocytes in the presence of recombinant human granulocyte macrophage-colony stimulating factor (rhGM-CSF) and recombinant human interleukin-4 (rhIL-4), while mature MDDCs were obtained by cultivation of immature MDDCs with lipopolysaccharide (LPS). PTX (200 mu g/ml) was added at the beginning of cell cultivation. We found that PTX significantly impaired differentiation and function of immature NIDDCs, as judged by the reduced allostimulatory activity of these cells on allogeneic T cells and down-regulation of costimulatory and adhesion molecules, such as CD86, CD40 and CD54. The maturation of MDDCs in the presence of PTX and LPS was characterized by the decreased expression of maturation marker CD83 and costimulatory molecule CD86, as well as lower stimulation of alloreactive T cells compared to the control MDDCs cultivated with LPS alone. PTX-treated MDDCs which were induced to mature with LPS produced lower levels of TNF-alpha, IL-12 and IL-18 and higher levels of IL-10 than corresponding control NIDDCs. PTX did not significantly alter endocytosis of dextran by both immature and mature MDDCs. Cumulatively, our results show for the first time that PTX might impair differentiation, maturation and function of human MDDCs in vitro, suggesting an additional mechanism of its immunomodulatory activity.
PB  - Elsevier Science BV, Amsterdam
T2  - International Immunopharmacology
T1  - Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro
VL  - 7
IS  - 2
SP  - 167
EP  - 174
DO  - 10.1016/j.intimp.2006.09.005
ER  - 

@article{
author = "Stojić-Vukanić, Zorica and Čolić, Miodrag and Dimitrijević, M.",
year = "2007",
abstract = "Pentoxifylline (PTX) is a drug used for the treatment of vascular disorders, but it also has a positive therapeutic effect in experimental models of some autoimmune diseases. In this work, we studied the effect of PTX on human monocyte-derived dendritic cells (MDDCs). Immature MDDCs were generated in vitro from monocytes in the presence of recombinant human granulocyte macrophage-colony stimulating factor (rhGM-CSF) and recombinant human interleukin-4 (rhIL-4), while mature MDDCs were obtained by cultivation of immature MDDCs with lipopolysaccharide (LPS). PTX (200 mu g/ml) was added at the beginning of cell cultivation. We found that PTX significantly impaired differentiation and function of immature NIDDCs, as judged by the reduced allostimulatory activity of these cells on allogeneic T cells and down-regulation of costimulatory and adhesion molecules, such as CD86, CD40 and CD54. The maturation of MDDCs in the presence of PTX and LPS was characterized by the decreased expression of maturation marker CD83 and costimulatory molecule CD86, as well as lower stimulation of alloreactive T cells compared to the control MDDCs cultivated with LPS alone. PTX-treated MDDCs which were induced to mature with LPS produced lower levels of TNF-alpha, IL-12 and IL-18 and higher levels of IL-10 than corresponding control NIDDCs. PTX did not significantly alter endocytosis of dextran by both immature and mature MDDCs. Cumulatively, our results show for the first time that PTX might impair differentiation, maturation and function of human MDDCs in vitro, suggesting an additional mechanism of its immunomodulatory activity.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Immunopharmacology",
title = "Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro",
volume = "7",
number = "2",
pages = "167-174",
doi = "10.1016/j.intimp.2006.09.005"
}

Stojić-Vukanić, Z., Čolić, M.,& Dimitrijević, M.. (2007). Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro. in International Immunopharmacology
Elsevier Science BV, Amsterdam., 7(2), 167-174.
https://doi.org/10.1016/j.intimp.2006.09.005

Stojić-Vukanić Z, Čolić M, Dimitrijević M. Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro. in International Immunopharmacology. 2007;7(2):167-174.
doi:10.1016/j.intimp.2006.09.005 .

Stojić-Vukanić, Zorica, Čolić, Miodrag, Dimitrijević, M., "Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro" in International Immunopharmacology, 7, no. 2 (2007):167-174,
https://doi.org/10.1016/j.intimp.2006.09.005 . .

TY  - CONF
AU  - Stojić-Vukanić, Zorica
AU  - Čolić, Miodrag
AU  - Backović, A.
AU  - Antić-Stanković, Jelena
AU  - Dimitrijević, M.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/684
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro
T1  - In vitro efekat pentoksifilina na diferencijaciju i maturaciju humanih dendritičnih ćelija monocitnog porekla
VL  - 56
IS  - 4
SP  - 410
EP  - 411
UR  - https://hdl.handle.net/21.15107/rcub_farfar_684
ER  - 

@conference{
author = "Stojić-Vukanić, Zorica and Čolić, Miodrag and Backović, A. and Antić-Stanković, Jelena and Dimitrijević, M.",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro, In vitro efekat pentoksifilina na diferencijaciju i maturaciju humanih dendritičnih ćelija monocitnog porekla",
volume = "56",
number = "4",
pages = "410-411",
url = "https://hdl.handle.net/21.15107/rcub_farfar_684"
}

Stojić-Vukanić, Z., Čolić, M., Backović, A., Antić-Stanković, J.,& Dimitrijević, M.. (2006). Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(4), 410-411.
https://hdl.handle.net/21.15107/rcub_farfar_684

Stojić-Vukanić Z, Čolić M, Backović A, Antić-Stanković J, Dimitrijević M. Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro. in Arhiv za farmaciju. 2006;56(4):410-411.
https://hdl.handle.net/21.15107/rcub_farfar_684 .

Stojić-Vukanić, Zorica, Čolić, Miodrag, Backović, A., Antić-Stanković, Jelena, Dimitrijević, M., "Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro" in Arhiv za farmaciju, 56, no. 4 (2006):410-411,
https://hdl.handle.net/21.15107/rcub_farfar_684 .

TY  - JOUR
AU  - Kovačević, Nada
AU  - Čolić, Miodrag
AU  - Backović, Aleksandar
AU  - Došlov-Kokoruš, Zvezdana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/674
AB  - The effect of the methanolic extract of root and rhizome of Epimedium alpinum (MEEA) on phenotype and functions of rat lymphocytes in vitro was studied. It has been found that MEEA at lower concentrations (0.1 mu g/ml and 1 mu g/ml) significantly enhanced proliferation of splenocytes and thymocytes triggered by concanavalin A (Con A), whereas higher concentrations of the extract (50 mu g/ ml-500 mu g/ml) were inhibitory. The stimulatory effect of MEEA on Con A-induced proliferation of splenocytes correlated with the up-regulation of interleukin-2 receptor alpha (IL-2R alpha) expression. In addition, increased production of IL-2 was observed when a blocking IL-2R alpha monoclonal antibody (mAb) was added to cell cultures. MEEA-suppressed proliferation of splenocytes was due to the inhibition of IL-2 production, the down-regulation of IL-2Ra expression and the induction of apoptosis. Cellular proliferation in the presence of inhibitory concentrations of MEEA higher than 50 mu g/ml could not be restored by the addition of exogenous IL-2.
PB  - Elsevier Science BV, Amsterdam
T2  - Fitoterapia
T1  - Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro
VL  - 77
IS  - 7-8
SP  - 561
EP  - 567
DO  - 10.1016/j.fitote.2006.09.008
ER  - 

@article{
author = "Kovačević, Nada and Čolić, Miodrag and Backović, Aleksandar and Došlov-Kokoruš, Zvezdana",
year = "2006",
abstract = "The effect of the methanolic extract of root and rhizome of Epimedium alpinum (MEEA) on phenotype and functions of rat lymphocytes in vitro was studied. It has been found that MEEA at lower concentrations (0.1 mu g/ml and 1 mu g/ml) significantly enhanced proliferation of splenocytes and thymocytes triggered by concanavalin A (Con A), whereas higher concentrations of the extract (50 mu g/ ml-500 mu g/ml) were inhibitory. The stimulatory effect of MEEA on Con A-induced proliferation of splenocytes correlated with the up-regulation of interleukin-2 receptor alpha (IL-2R alpha) expression. In addition, increased production of IL-2 was observed when a blocking IL-2R alpha monoclonal antibody (mAb) was added to cell cultures. MEEA-suppressed proliferation of splenocytes was due to the inhibition of IL-2 production, the down-regulation of IL-2Ra expression and the induction of apoptosis. Cellular proliferation in the presence of inhibitory concentrations of MEEA higher than 50 mu g/ml could not be restored by the addition of exogenous IL-2.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Fitoterapia",
title = "Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro",
volume = "77",
number = "7-8",
pages = "561-567",
doi = "10.1016/j.fitote.2006.09.008"
}

Kovačević, N., Čolić, M., Backović, A.,& Došlov-Kokoruš, Z.. (2006). Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro. in Fitoterapia
Elsevier Science BV, Amsterdam., 77(7-8), 561-567.
https://doi.org/10.1016/j.fitote.2006.09.008

Kovačević N, Čolić M, Backović A, Došlov-Kokoruš Z. Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro. in Fitoterapia. 2006;77(7-8):561-567.
doi:10.1016/j.fitote.2006.09.008 .

Kovačević, Nada, Čolić, Miodrag, Backović, Aleksandar, Došlov-Kokoruš, Zvezdana, "Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro" in Fitoterapia, 77, no. 7-8 (2006):561-567,
https://doi.org/10.1016/j.fitote.2006.09.008 . .

TY  - CONF
AU  - Vasilev, S.
AU  - Majstorović, Ivana
AU  - Vucević, D.
AU  - Gašić, Sonja
AU  - Vasilijić, Saša
AU  - Bufan, Biljana
AU  - Ćupić, Vitomir
AU  - Čolić, Miodrag
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/637
PB  - Elsevier Ireland Ltd, Clare
C3  - Toxicology Letters
T1  - The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro
VL  - 158
IS  - 1, Supplement
SP  - S229
EP  - S229
DO  - 10.1016/j.toxlet.2005.05.014
ER  - 

@conference{
author = "Vasilev, S. and Majstorović, Ivana and Vucević, D. and Gašić, Sonja and Vasilijić, Saša and Bufan, Biljana and Ćupić, Vitomir and Čolić, Miodrag",
year = "2005",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Toxicology Letters",
title = "The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro",
volume = "158",
number = "1, Supplement",
pages = "S229-S229",
doi = "10.1016/j.toxlet.2005.05.014"
}

Vasilev, S., Majstorović, I., Vucević, D., Gašić, S., Vasilijić, S., Bufan, B., Ćupić, V.,& Čolić, M.. (2005). The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro. in Toxicology Letters
Elsevier Ireland Ltd, Clare., 158(1, Supplement), S229-S229.
https://doi.org/10.1016/j.toxlet.2005.05.014

Vasilev S, Majstorović I, Vucević D, Gašić S, Vasilijić S, Bufan B, Ćupić V, Čolić M. The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro. in Toxicology Letters. 2005;158(1, Supplement):S229-S229.
doi:10.1016/j.toxlet.2005.05.014 .

Vasilev, S., Majstorović, Ivana, Vucević, D., Gašić, Sonja, Vasilijić, Saša, Bufan, Biljana, Ćupić, Vitomir, Čolić, Miodrag, "The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro" in Toxicology Letters, 158, no. 1, Supplement (2005):S229-S229,
https://doi.org/10.1016/j.toxlet.2005.05.014 . .

TY  - JOUR
AU  - Milenković, Marina
AU  - Vučićević, Dragana
AU  - Milosavljević, P
AU  - Arsenović-Ranin, Nevena
AU  - Stojić-Vukanić, Zorica
AU  - Čolić, Miodrag
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/595
AB  - Fusidic acid and its sodium salt sodium fusidate (fusidin) are widely used antibiotics that possess immunomodulating properties. It has been shown that fusidin ameliorates the course of several organ-specific immunoinflammatory diseases and thus we investigated the effect of fusidin on myosin-induced experimental autoimmune myocarditis (EAM) in rats, a well-established animal model for human giant cell myocarditis and postmyocarditis dilated cardiomyopathy (DCM). Fusidin at doses of 80 mg kg(-1) was administrated i.m. to male Dark Agouti (DA) rats, either from days 0 to 10 (early treatment group), or from days 10 to 20 (late treatment group) after induction of EAM. Efficacy of fusidin treatment was determined on day 21 of EAM development. It was observed that both early and late treatment with fusidin markedly ameliorated clinical, histological and immunohistochemical signs of the disease. The treated rats had significantly decreased incidence of EAM, heart weight and heart weight/body weight ratio (Hw/Bw) compared with untreated animals. In contrast to the severe myocardial damage and cellular infiltration in the EAM rats, there was only focal infiltration of inflammatory cells in the myocardium of the treated rats. In both treated groups the mean microscopic score was markedly lower compared with vehicle-treated animals. In addition, the number of CD4+, ED1+ and OX6+ cells was significantly lower in myocardium of fusidin-treated rats than that in untreated group. The present findings suggest that fusidin exhibited both early and late therapeutical effect in EAM.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Pharmacological Research
T1  - Suppression of experimental autoimmune myocarditis by sodium fusidate (fusidin)
VL  - 52
IS  - 6
SP  - 491
EP  - 496
DO  - 10.1016/j.phrs.2005.08.001
ER  - 

@article{
author = "Milenković, Marina and Vučićević, Dragana and Milosavljević, P and Arsenović-Ranin, Nevena and Stojić-Vukanić, Zorica and Čolić, Miodrag",
year = "2005",
abstract = "Fusidic acid and its sodium salt sodium fusidate (fusidin) are widely used antibiotics that possess immunomodulating properties. It has been shown that fusidin ameliorates the course of several organ-specific immunoinflammatory diseases and thus we investigated the effect of fusidin on myosin-induced experimental autoimmune myocarditis (EAM) in rats, a well-established animal model for human giant cell myocarditis and postmyocarditis dilated cardiomyopathy (DCM). Fusidin at doses of 80 mg kg(-1) was administrated i.m. to male Dark Agouti (DA) rats, either from days 0 to 10 (early treatment group), or from days 10 to 20 (late treatment group) after induction of EAM. Efficacy of fusidin treatment was determined on day 21 of EAM development. It was observed that both early and late treatment with fusidin markedly ameliorated clinical, histological and immunohistochemical signs of the disease. The treated rats had significantly decreased incidence of EAM, heart weight and heart weight/body weight ratio (Hw/Bw) compared with untreated animals. In contrast to the severe myocardial damage and cellular infiltration in the EAM rats, there was only focal infiltration of inflammatory cells in the myocardium of the treated rats. In both treated groups the mean microscopic score was markedly lower compared with vehicle-treated animals. In addition, the number of CD4+, ED1+ and OX6+ cells was significantly lower in myocardium of fusidin-treated rats than that in untreated group. The present findings suggest that fusidin exhibited both early and late therapeutical effect in EAM.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Pharmacological Research",
title = "Suppression of experimental autoimmune myocarditis by sodium fusidate (fusidin)",
volume = "52",
number = "6",
pages = "491-496",
doi = "10.1016/j.phrs.2005.08.001"
}

Milenković, M., Vučićević, D., Milosavljević, P., Arsenović-Ranin, N., Stojić-Vukanić, Z.,& Čolić, M.. (2005). Suppression of experimental autoimmune myocarditis by sodium fusidate (fusidin). in Pharmacological Research
Academic Press Ltd- Elsevier Science Ltd, London., 52(6), 491-496.
https://doi.org/10.1016/j.phrs.2005.08.001

Milenković M, Vučićević D, Milosavljević P, Arsenović-Ranin N, Stojić-Vukanić Z, Čolić M. Suppression of experimental autoimmune myocarditis by sodium fusidate (fusidin). in Pharmacological Research. 2005;52(6):491-496.
doi:10.1016/j.phrs.2005.08.001 .

Milenković, Marina, Vučićević, Dragana, Milosavljević, P, Arsenović-Ranin, Nevena, Stojić-Vukanić, Zorica, Čolić, Miodrag, "Suppression of experimental autoimmune myocarditis by sodium fusidate (fusidin)" in Pharmacological Research, 52, no. 6 (2005):491-496,
https://doi.org/10.1016/j.phrs.2005.08.001 . .

TY  - JOUR
AU  - Cerović, Aleksandra
AU  - Miletić, Ivanka
AU  - Blagojević, Duško
AU  - Šobajić, Slađana
AU  - Čolić, Miodrag
AU  - Vasiljevska, Milijana
AU  - Poznanić, Milica
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/652
AB  - The effect of dietary zinc on the distribution of fatty acids in blood plasma chylomicrons was studied. The experiment was performed on male Mongolian Gerbils, fed ad libitum for 3 weeks with standard diet, containing 8 or 38 mg of Zn per kg of food (low zinc diet group, LZ, and saturated zinc diet group, SZ, respectivelly). At the 21st day gerbils were given sunflower oil by gavage. After 2.5 hours, blood was collected from abdominal vein, and samples were pooled (five animals per one sample). Chylomicron fractions were isolated by ultracentrifugation and mass of dried chylomicrons was measured. Fatty acid composition was analyzed by gas-liquid chromatography. Significantly higher amount of chylomicrons in blood plasma of SZ than in LZ group was found. In chylomicrons, following fatty acids were found: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 and 20:4. The amount of individual fatty acids in chylomicrons in both groups was similar, except 20:4 where lower amount in SZ group was found. Zinc diet did not affect fatty acid distribution in chylomicrons of both groups. Animals fed with zinc saturated diet had higher amount of fatty acids in blood plasma. Observed results suggest that dietary zinc influences the quantity of fatty acids absorption but not its distribution in chylomicrons.
AB  - Ispitivan je efekat unosa dijetarnog cinka na distribuciju masnih kiselina u hilomikronima plazme. Eksperiment je rađen na mužjacima gerbila, hranjenim standardnom dijetom, ad libitum, u toku 3 nedelje hrana je sadržala 8 ili 38 mg cinka po kg hrane (grupa sa smanjenim unosom cinka, LZ i grupa sa povećanim unosom cinka, SZ, respektivno). Dvadeset prvog dana, životinjama je dato suncokretovo ulje sondom. Nakon 2,5 časa, krv je uzimana iz abdominalne vene i uzorci su pulovani (5 životinja za jedan uzorak). Hilomikronske frakcije su izolovane ultracentrifugiranjem i merena je masa suvih hilomikrona. Masnokiselinski sastav lipida u hilomikronima je određivan gasno-tečnom hromatografijom. Nađena je značajno veća količina hilomikrona u plazmi SZ grupe nego u LZ grupi. U hilomikronima su nađene sledeće masne kiseline: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 i 20:4. Zastupljenost pojedinačnih masnih kiselina u hilomikronima obe grupe bila je slična, izuzev za 20:4 gde je izmerena niža količina u SZ grupi. Ishrana cinkom ne utiče na distribuciju većine masnih kiselina u hilomikronima. Dobijeni rezultati ukazuju da cink utiče na metabolizam masti i to u delu formiranja hilomikrona, ali je uticaj ograničen na količinu formiranih hilomikrona, ali ne i distribuciju pojedinih masnih kiselina u hilomikronima.
PB  - Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Jugoslovenska medicinska biohemija
T1  - The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons
T1  - Efekat ishrane cinkom na distribuciju masnih kiselina u hilomikronima plazme
VL  - 24
IS  - 1
SP  - 15
EP  - 20
DO  - 10.2298/JMH0501015C
ER  - 

@article{
author = "Cerović, Aleksandra and Miletić, Ivanka and Blagojević, Duško and Šobajić, Slađana and Čolić, Miodrag and Vasiljevska, Milijana and Poznanić, Milica",
year = "2005",
abstract = "The effect of dietary zinc on the distribution of fatty acids in blood plasma chylomicrons was studied. The experiment was performed on male Mongolian Gerbils, fed ad libitum for 3 weeks with standard diet, containing 8 or 38 mg of Zn per kg of food (low zinc diet group, LZ, and saturated zinc diet group, SZ, respectivelly). At the 21st day gerbils were given sunflower oil by gavage. After 2.5 hours, blood was collected from abdominal vein, and samples were pooled (five animals per one sample). Chylomicron fractions were isolated by ultracentrifugation and mass of dried chylomicrons was measured. Fatty acid composition was analyzed by gas-liquid chromatography. Significantly higher amount of chylomicrons in blood plasma of SZ than in LZ group was found. In chylomicrons, following fatty acids were found: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 and 20:4. The amount of individual fatty acids in chylomicrons in both groups was similar, except 20:4 where lower amount in SZ group was found. Zinc diet did not affect fatty acid distribution in chylomicrons of both groups. Animals fed with zinc saturated diet had higher amount of fatty acids in blood plasma. Observed results suggest that dietary zinc influences the quantity of fatty acids absorption but not its distribution in chylomicrons., Ispitivan je efekat unosa dijetarnog cinka na distribuciju masnih kiselina u hilomikronima plazme. Eksperiment je rađen na mužjacima gerbila, hranjenim standardnom dijetom, ad libitum, u toku 3 nedelje hrana je sadržala 8 ili 38 mg cinka po kg hrane (grupa sa smanjenim unosom cinka, LZ i grupa sa povećanim unosom cinka, SZ, respektivno). Dvadeset prvog dana, životinjama je dato suncokretovo ulje sondom. Nakon 2,5 časa, krv je uzimana iz abdominalne vene i uzorci su pulovani (5 životinja za jedan uzorak). Hilomikronske frakcije su izolovane ultracentrifugiranjem i merena je masa suvih hilomikrona. Masnokiselinski sastav lipida u hilomikronima je određivan gasno-tečnom hromatografijom. Nađena je značajno veća količina hilomikrona u plazmi SZ grupe nego u LZ grupi. U hilomikronima su nađene sledeće masne kiseline: 16:0, 16:1, 17:0, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 21:0 i 20:4. Zastupljenost pojedinačnih masnih kiselina u hilomikronima obe grupe bila je slična, izuzev za 20:4 gde je izmerena niža količina u SZ grupi. Ishrana cinkom ne utiče na distribuciju većine masnih kiselina u hilomikronima. Dobijeni rezultati ukazuju da cink utiče na metabolizam masti i to u delu formiranja hilomikrona, ali je uticaj ograničen na količinu formiranih hilomikrona, ali ne i distribuciju pojedinih masnih kiselina u hilomikronima.",
publisher = "Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Jugoslovenska medicinska biohemija",
title = "The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons, Efekat ishrane cinkom na distribuciju masnih kiselina u hilomikronima plazme",
volume = "24",
number = "1",
pages = "15-20",
doi = "10.2298/JMH0501015C"
}

Cerović, A., Miletić, I., Blagojević, D., Šobajić, S., Čolić, M., Vasiljevska, M.,& Poznanić, M.. (2005). The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons. in Jugoslovenska medicinska biohemija
Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd., 24(1), 15-20.
https://doi.org/10.2298/JMH0501015C

Cerović A, Miletić I, Blagojević D, Šobajić S, Čolić M, Vasiljevska M, Poznanić M. The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons. in Jugoslovenska medicinska biohemija. 2005;24(1):15-20.
doi:10.2298/JMH0501015C .

Cerović, Aleksandra, Miletić, Ivanka, Blagojević, Duško, Šobajić, Slađana, Čolić, Miodrag, Vasiljevska, Milijana, Poznanić, Milica, "The effect of zinc diet on distribution of fatty acid in blood plasma chylomicrons" in Jugoslovenska medicinska biohemija, 24, no. 1 (2005):15-20,
https://doi.org/10.2298/JMH0501015C . .

TY  - JOUR
AU  - Antić-Stanković, Jelena
AU  - Vucević, D
AU  - Majstorović, I
AU  - Vasilijić, Saša
AU  - Čolić, Miodrag
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/508
AB  - In our previous work we showed that 3F10 monoclonal antibody (mAb), which recognizes the rat complement receptor 1-related/gene protein y (Crry), induces homotipic aggregation of thymocytes. In this work we studied the effect of 3F10 mAb on proliferation of rat thymocytes stimulated with concanavalin A (ConA) or by cross-linking the T cell receptor (TCR) by anti-alphabetaTCR mAb (R73), in vitro, and the mechanisms involved in the process. Our results show that 3F10 mAb stimulates proliferation of total thymocytes triggered by suboptimal concentrations of ConA or TCR cross-linking, in a dose-dependent manner. Maximal stimulation was observed using 10 mug/ml and 20 mug/Ml of 3F10 mAb, respectively. The 3F10-induced stimulation of thymocytes proliferation in the presence of ConA, that was followed by increased production of interleukin-2 (IL-2), up-regulation of the expression of IL-2 receptor alpha (IL-2Ralpha) and was inhibited by anti-CD11a and anti-CD 18 mAbs. Purified thymocytes did not respond by proliferation to 3F10 mAb, either alone or in combination with R73 mAb or ConA. Proliferation of these cells was achieved only in the presence of OX-6(+) antigen-presenting cells (APC) and additional signals transmitted by TCR or ConA. These results suggest that Crry is involved in the LFA-1 dependent proliferation of thymocytes, a phenomenon that has not been recognized so far.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Life Sciences
T1  - The role of rat Crry, a complement regulatory protein, in proliferation of thymocytes
VL  - 75
IS  - 25
SP  - 3053
EP  - 3062
DO  - 10.1016/j.lfs.2004.06.007
ER  - 

@article{
author = "Antić-Stanković, Jelena and Vucević, D and Majstorović, I and Vasilijić, Saša and Čolić, Miodrag",
year = "2004",
abstract = "In our previous work we showed that 3F10 monoclonal antibody (mAb), which recognizes the rat complement receptor 1-related/gene protein y (Crry), induces homotipic aggregation of thymocytes. In this work we studied the effect of 3F10 mAb on proliferation of rat thymocytes stimulated with concanavalin A (ConA) or by cross-linking the T cell receptor (TCR) by anti-alphabetaTCR mAb (R73), in vitro, and the mechanisms involved in the process. Our results show that 3F10 mAb stimulates proliferation of total thymocytes triggered by suboptimal concentrations of ConA or TCR cross-linking, in a dose-dependent manner. Maximal stimulation was observed using 10 mug/ml and 20 mug/Ml of 3F10 mAb, respectively. The 3F10-induced stimulation of thymocytes proliferation in the presence of ConA, that was followed by increased production of interleukin-2 (IL-2), up-regulation of the expression of IL-2 receptor alpha (IL-2Ralpha) and was inhibited by anti-CD11a and anti-CD 18 mAbs. Purified thymocytes did not respond by proliferation to 3F10 mAb, either alone or in combination with R73 mAb or ConA. Proliferation of these cells was achieved only in the presence of OX-6(+) antigen-presenting cells (APC) and additional signals transmitted by TCR or ConA. These results suggest that Crry is involved in the LFA-1 dependent proliferation of thymocytes, a phenomenon that has not been recognized so far.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Life Sciences",
title = "The role of rat Crry, a complement regulatory protein, in proliferation of thymocytes",
volume = "75",
number = "25",
pages = "3053-3062",
doi = "10.1016/j.lfs.2004.06.007"
}

Antić-Stanković, J., Vucević, D., Majstorović, I., Vasilijić, S.,& Čolić, M.. (2004). The role of rat Crry, a complement regulatory protein, in proliferation of thymocytes. in Life Sciences
Pergamon-Elsevier Science Ltd, Oxford., 75(25), 3053-3062.
https://doi.org/10.1016/j.lfs.2004.06.007

Antić-Stanković J, Vucević D, Majstorović I, Vasilijić S, Čolić M. The role of rat Crry, a complement regulatory protein, in proliferation of thymocytes. in Life Sciences. 2004;75(25):3053-3062.
doi:10.1016/j.lfs.2004.06.007 .

Antić-Stanković, Jelena, Vucević, D, Majstorović, I, Vasilijić, Saša, Čolić, Miodrag, "The role of rat Crry, a complement regulatory protein, in proliferation of thymocytes" in Life Sciences, 75, no. 25 (2004):3053-3062,
https://doi.org/10.1016/j.lfs.2004.06.007 . .

TY  - JOUR
AU  - Čolić, Miodrag
AU  - Mojsilović, Slavko
AU  - Pavlović, Bojan
AU  - Vučićević, Dragana
AU  - Majstorović, Ivana
AU  - Bufan, Biljana
AU  - Stojić-Vukanić, Zorica
AU  - Vasilijić, Saša
AU  - Vučević, Dragana
AU  - Gašić, Sonja
AU  - Balint, Bela
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/547
AB  - Background. Dendritic cells (DC) have been used for immunotherapy of malignant tumors, different kinds of infections, and other clinical conditions. For that purpose, optimal conditions for the generation of functionally mature DC in vitro are required. Two different protocols for the induction of maturation of monocyte-derived DC (MDDC) were compared in this study. Methods. MDDC were generated in vitro by cultivating adherent monocytes of healthy volunteers with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin 4 (IL-4) during 6-days period. The immature DC thus prepared were induced to mature using two protocols. DC were stimulated for 2 days with lipopolysaccharide (LPS), or with a cocktail of proinflammatory mediators (PM) containing IL-1b, IL-6, tumor necrosis factor α (TNFα), and prostaglandin E2 (PGE2), respectively. Phenotypic characteristics of MDDC and their endocytic activity were studied by flow cytometry. Allostimulatory activity of these cells was tested in the mixed leukocyte reaction (MLR), whereas the production of cytokines was determined by ELISA kits. Results. MDDC matured with PM (PM-DC) were predominantly non-adherent cells, while about 30% of LPS-matured DC were adherent cells. In comparison with LPS-DC, PM-DC expressed higher levels of CD86 and CD83, had lower endocytic activity, produced higher levels of IL-10 and lower levels of IL-12, and more strongly stimulated proliferation of allogeneic lymphocytes. Conclusion The protocol based on the combination of proinflammatory cytokines and PGE2 is better for the induction of maturation of human MDDC in vitro than the protocol using LPS alone.
AB  - Uvod. Dendritične ćelije (DC) se koriste u imunoterapiji malignih tumora, različitih vrsta infekcija i drugih oboljenja. U tom cilju neophodni su optimalni uslovi za dobijanje funkcionalno zrelih DC in vitro. U ovom radu smo poredili dva različita protokola za indukciju maturacije DC monocitnog porekla (MDDC). Metode. MDDC su dobijene in vitro kultivisanjem adherentnih monocita zdravih dobrovoljnih davalaca krvi pomoću faktora stimulacije granulocitno-makrofagnih kolonija (GM-CSF) i interleukina-4 (IL-4) u toku 6 dana. Tako pripremljene nezrele DC su indukovane na sazrevanje pomoću dva protokola. DC su stimulisane u toku 2 dana lipopolisaharidom (LPS) ili koktelom proinflamatornih medijatora (PM) koji je sadržavao IL-1b, IL-6, faktor nekroze tumora α (TNFα) i prostaglandin E2 (PGE2). Fenotipske karakteristike MDDC i njihova endocitozna aktivnost su ispitivani pomoću protočne citometrije. Alostimulatorna aktivnost ovih ćelija je ispitivana u testu mešane leukocitne reakcije (MLR), dok je stvaranje citokina određivano ELISA kompletima. Rezultati. MDDC koje su sazrevale u prisustvu PM (PM-DC) su bile predominantno neadherentne ćelije, dok su oko 30% DC koje su sazrevale pod uticajem LPS (LPS-DC) bile adherentne. U poređenju sa LPS-DC, PM-DC su ispoljavale više nivoe CD86 i CD83 molekula, imale slabiju endocitoznu aktivnost, produkovale više IL-10, a manje IL-12 i snažnije stimulisale proliferaciju alogenih limfocita. Zaključak. Protokol koji se bazira na primeni kombinacije proinflamatornih citokina i PGE2 je bolji za indukciju maturacije humanih MDDC in vitro nego LPS protokol.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Comparison of two different protocols for the induction of maturation of human dendritic cells in vitro
T1  - Poređenje dva različita protokola za indukciju maturacije humanih dendritičnih ćelija in vitro
VL  - 61
IS  - 5
SP  - 471
EP  - 478
DO  - 10.2298/VSP0405471C
ER  - 

@article{
author = "Čolić, Miodrag and Mojsilović, Slavko and Pavlović, Bojan and Vučićević, Dragana and Majstorović, Ivana and Bufan, Biljana and Stojić-Vukanić, Zorica and Vasilijić, Saša and Vučević, Dragana and Gašić, Sonja and Balint, Bela",
year = "2004",
abstract = "Background. Dendritic cells (DC) have been used for immunotherapy of malignant tumors, different kinds of infections, and other clinical conditions. For that purpose, optimal conditions for the generation of functionally mature DC in vitro are required. Two different protocols for the induction of maturation of monocyte-derived DC (MDDC) were compared in this study. Methods. MDDC were generated in vitro by cultivating adherent monocytes of healthy volunteers with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin 4 (IL-4) during 6-days period. The immature DC thus prepared were induced to mature using two protocols. DC were stimulated for 2 days with lipopolysaccharide (LPS), or with a cocktail of proinflammatory mediators (PM) containing IL-1b, IL-6, tumor necrosis factor α (TNFα), and prostaglandin E2 (PGE2), respectively. Phenotypic characteristics of MDDC and their endocytic activity were studied by flow cytometry. Allostimulatory activity of these cells was tested in the mixed leukocyte reaction (MLR), whereas the production of cytokines was determined by ELISA kits. Results. MDDC matured with PM (PM-DC) were predominantly non-adherent cells, while about 30% of LPS-matured DC were adherent cells. In comparison with LPS-DC, PM-DC expressed higher levels of CD86 and CD83, had lower endocytic activity, produced higher levels of IL-10 and lower levels of IL-12, and more strongly stimulated proliferation of allogeneic lymphocytes. Conclusion The protocol based on the combination of proinflammatory cytokines and PGE2 is better for the induction of maturation of human MDDC in vitro than the protocol using LPS alone., Uvod. Dendritične ćelije (DC) se koriste u imunoterapiji malignih tumora, različitih vrsta infekcija i drugih oboljenja. U tom cilju neophodni su optimalni uslovi za dobijanje funkcionalno zrelih DC in vitro. U ovom radu smo poredili dva različita protokola za indukciju maturacije DC monocitnog porekla (MDDC). Metode. MDDC su dobijene in vitro kultivisanjem adherentnih monocita zdravih dobrovoljnih davalaca krvi pomoću faktora stimulacije granulocitno-makrofagnih kolonija (GM-CSF) i interleukina-4 (IL-4) u toku 6 dana. Tako pripremljene nezrele DC su indukovane na sazrevanje pomoću dva protokola. DC su stimulisane u toku 2 dana lipopolisaharidom (LPS) ili koktelom proinflamatornih medijatora (PM) koji je sadržavao IL-1b, IL-6, faktor nekroze tumora α (TNFα) i prostaglandin E2 (PGE2). Fenotipske karakteristike MDDC i njihova endocitozna aktivnost su ispitivani pomoću protočne citometrije. Alostimulatorna aktivnost ovih ćelija je ispitivana u testu mešane leukocitne reakcije (MLR), dok je stvaranje citokina određivano ELISA kompletima. Rezultati. MDDC koje su sazrevale u prisustvu PM (PM-DC) su bile predominantno neadherentne ćelije, dok su oko 30% DC koje su sazrevale pod uticajem LPS (LPS-DC) bile adherentne. U poređenju sa LPS-DC, PM-DC su ispoljavale više nivoe CD86 i CD83 molekula, imale slabiju endocitoznu aktivnost, produkovale više IL-10, a manje IL-12 i snažnije stimulisale proliferaciju alogenih limfocita. Zaključak. Protokol koji se bazira na primeni kombinacije proinflamatornih citokina i PGE2 je bolji za indukciju maturacije humanih MDDC in vitro nego LPS protokol.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Comparison of two different protocols for the induction of maturation of human dendritic cells in vitro, Poređenje dva različita protokola za indukciju maturacije humanih dendritičnih ćelija in vitro",
volume = "61",
number = "5",
pages = "471-478",
doi = "10.2298/VSP0405471C"
}

Čolić, M., Mojsilović, S., Pavlović, B., Vučićević, D., Majstorović, I., Bufan, B., Stojić-Vukanić, Z., Vasilijić, S., Vučević, D., Gašić, S.,& Balint, B.. (2004). Comparison of two different protocols for the induction of maturation of human dendritic cells in vitro. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 61(5), 471-478.
https://doi.org/10.2298/VSP0405471C

Čolić M, Mojsilović S, Pavlović B, Vučićević D, Majstorović I, Bufan B, Stojić-Vukanić Z, Vasilijić S, Vučević D, Gašić S, Balint B. Comparison of two different protocols for the induction of maturation of human dendritic cells in vitro. in Vojnosanitetski pregled. 2004;61(5):471-478.
doi:10.2298/VSP0405471C .

Čolić, Miodrag, Mojsilović, Slavko, Pavlović, Bojan, Vučićević, Dragana, Majstorović, Ivana, Bufan, Biljana, Stojić-Vukanić, Zorica, Vasilijić, Saša, Vučević, Dragana, Gašić, Sonja, Balint, Bela, "Comparison of two different protocols for the induction of maturation of human dendritic cells in vitro" in Vojnosanitetski pregled, 61, no. 5 (2004):471-478,
https://doi.org/10.2298/VSP0405471C . .

TY  - JOUR
AU  - Vasiljević, Ivana D.
AU  - Jovanović, Marina
AU  - Čolić, Miodrag
AU  - Mihajlović, Rosa
AU  - Đukić, Mirjana
AU  - Ninković, Milica
AU  - Maličević, Živorad
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/562
AB  - The aetiology of neuronal death in neurodegenerative diseases, including Huntington-s disease, is still unknown. There could be a complex interplay among altered energy metabolism, excitotoxicity and oxidative stress. Our aim was to examine the effects of intrastriatal injection of a selective inhibitor of neuronal nitric oxide synthase, 7-nitroindazole, and a non-specific potent nitric oxide synthase inhibitor, Nw-nitro-L-arginine methyl ester, in order to study the possible involvement of glutathione, an important antioxidant, in quinolinic acid-induced striatal toxicity in the rat. Unilateral administration of quinolinic acid to rat striatum in a single dose of 150 nmol/L was used as a model of Huntington-s disease. The other group of animals were pretreated with 7- nitroindazole and Nw-nitro-L-arginine methyl ester, respectively. Control groups were treated with saline solution and olive oil, respectively. Content of total glutathione, was increased in the ipsi- and contralateral striatum, forebrain cortex, basal forebrain and hippocampus in the groups treated with nitric oxid synthase inhibitors and quinolinic acid compared to the quinolinic acid-treated animals. These results support the hypothesis that oxygen free radicals contribute to excitotoxic neuronal injury, and also that nitric oxide synthase inhibitors could be potential neuroprotective agents in Huntington-s disease.
AB  - Etiologija selektivnog umiranja neurona u neurodegenerativnim bolestima je nepoznata, iako postoje dokazi o defektu energetskog metabolizma, ekscitotoksičnosti i oksidativnom oštećenju. Verovatno je da ključnu ulogu ima kompleksna interakcija između ovih mehanizama. Cilj ovog rada bio je da se ispitaju efekti intrastrijatne primene selektivnog inhibitora neuronske azot oksid sintaze, 7-nitroindazola, kao i nespecifičnog inhibitora azot oksid sintaze, Nw-nitro-l-arginin metil estra, zbog moguće uključenosti glutationa, ključnog antioksidansa, u toksičnost strijatuma izazvanu hinolinskom kiselinom, kod pacova. Unilateralna aplikacija hinolinske kiseline, u strijatum pacova u pojedinačnoj dozi od 150 nmol/L korišćena je kao model Hantingtonove bolesti. Druge grupe životinja tretirane su 7-nitroindazolom, odnosno Nw-nitro-l-arginin metil estrom. Kontrolne grupe dobijale su fiziološki rastvor, odnosno maslinovo ulje. Sadržaj ukupnog glutationa je povećan u ipsi- i kontralateralnom strijatumu, kori prednjeg mozga, bazalnom prednjem mozgu i hipokampusu grupa životinja koje su pored hinolinske kiseline primile i odgovarajući inhibitor neuronske azot oksid sintaze, u poređenju sa grupom tretiranom samo neurotoksinom. Ovi podaci pokazuju da kiseonični slobodni radikali učestvuju u ekscitotoksičnom oštećenju neurona, kao i da inhibitori azot oksid sintaze mogu biti potencijalni neuroprotektivni agensi u Hantingtonovoj bolesti.
PB  - Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd
T2  - Jugoslovenska medicinska biohemija
T1  - Effects of various nitric oxide synthase inhibitors on quinolinic acid-induced neuronal injury in rats
T1  - Efekti različitih inhibitora azot oksid sintaze na oštećenje neurona indukovano hinolinskom kiselinom kod pacova
VL  - 23
IS  - 1
SP  - 11
EP  - 18
DO  - 10.2298/JMH0401011V
ER  - 

@article{
author = "Vasiljević, Ivana D. and Jovanović, Marina and Čolić, Miodrag and Mihajlović, Rosa and Đukić, Mirjana and Ninković, Milica and Maličević, Živorad",
year = "2004",
abstract = "The aetiology of neuronal death in neurodegenerative diseases, including Huntington-s disease, is still unknown. There could be a complex interplay among altered energy metabolism, excitotoxicity and oxidative stress. Our aim was to examine the effects of intrastriatal injection of a selective inhibitor of neuronal nitric oxide synthase, 7-nitroindazole, and a non-specific potent nitric oxide synthase inhibitor, Nw-nitro-L-arginine methyl ester, in order to study the possible involvement of glutathione, an important antioxidant, in quinolinic acid-induced striatal toxicity in the rat. Unilateral administration of quinolinic acid to rat striatum in a single dose of 150 nmol/L was used as a model of Huntington-s disease. The other group of animals were pretreated with 7- nitroindazole and Nw-nitro-L-arginine methyl ester, respectively. Control groups were treated with saline solution and olive oil, respectively. Content of total glutathione, was increased in the ipsi- and contralateral striatum, forebrain cortex, basal forebrain and hippocampus in the groups treated with nitric oxid synthase inhibitors and quinolinic acid compared to the quinolinic acid-treated animals. These results support the hypothesis that oxygen free radicals contribute to excitotoxic neuronal injury, and also that nitric oxide synthase inhibitors could be potential neuroprotective agents in Huntington-s disease., Etiologija selektivnog umiranja neurona u neurodegenerativnim bolestima je nepoznata, iako postoje dokazi o defektu energetskog metabolizma, ekscitotoksičnosti i oksidativnom oštećenju. Verovatno je da ključnu ulogu ima kompleksna interakcija između ovih mehanizama. Cilj ovog rada bio je da se ispitaju efekti intrastrijatne primene selektivnog inhibitora neuronske azot oksid sintaze, 7-nitroindazola, kao i nespecifičnog inhibitora azot oksid sintaze, Nw-nitro-l-arginin metil estra, zbog moguće uključenosti glutationa, ključnog antioksidansa, u toksičnost strijatuma izazvanu hinolinskom kiselinom, kod pacova. Unilateralna aplikacija hinolinske kiseline, u strijatum pacova u pojedinačnoj dozi od 150 nmol/L korišćena je kao model Hantingtonove bolesti. Druge grupe životinja tretirane su 7-nitroindazolom, odnosno Nw-nitro-l-arginin metil estrom. Kontrolne grupe dobijale su fiziološki rastvor, odnosno maslinovo ulje. Sadržaj ukupnog glutationa je povećan u ipsi- i kontralateralnom strijatumu, kori prednjeg mozga, bazalnom prednjem mozgu i hipokampusu grupa životinja koje su pored hinolinske kiseline primile i odgovarajući inhibitor neuronske azot oksid sintaze, u poređenju sa grupom tretiranom samo neurotoksinom. Ovi podaci pokazuju da kiseonični slobodni radikali učestvuju u ekscitotoksičnom oštećenju neurona, kao i da inhibitori azot oksid sintaze mogu biti potencijalni neuroprotektivni agensi u Hantingtonovoj bolesti.",
publisher = "Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd",
journal = "Jugoslovenska medicinska biohemija",
title = "Effects of various nitric oxide synthase inhibitors on quinolinic acid-induced neuronal injury in rats, Efekti različitih inhibitora azot oksid sintaze na oštećenje neurona indukovano hinolinskom kiselinom kod pacova",
volume = "23",
number = "1",
pages = "11-18",
doi = "10.2298/JMH0401011V"
}

Vasiljević, I. D., Jovanović, M., Čolić, M., Mihajlović, R., Đukić, M., Ninković, M.,& Maličević, Ž.. (2004). Effects of various nitric oxide synthase inhibitors on quinolinic acid-induced neuronal injury in rats. in Jugoslovenska medicinska biohemija
Društvo medicinskih biohemičara Srbije i Crne Gore, Beograd i Univerzitet u Beogradu - Farmaceutski fakultet, Beograd., 23(1), 11-18.
https://doi.org/10.2298/JMH0401011V

Vasiljević ID, Jovanović M, Čolić M, Mihajlović R, Đukić M, Ninković M, Maličević Ž. Effects of various nitric oxide synthase inhibitors on quinolinic acid-induced neuronal injury in rats. in Jugoslovenska medicinska biohemija. 2004;23(1):11-18.
doi:10.2298/JMH0401011V .

Vasiljević, Ivana D., Jovanović, Marina, Čolić, Miodrag, Mihajlović, Rosa, Đukić, Mirjana, Ninković, Milica, Maličević, Živorad, "Effects of various nitric oxide synthase inhibitors on quinolinic acid-induced neuronal injury in rats" in Jugoslovenska medicinska biohemija, 23, no. 1 (2004):11-18,
https://doi.org/10.2298/JMH0401011V . .

TY  - JOUR
AU  - Milenković, Marina
AU  - Milosavljević, Petar
AU  - Stojić-Vukanić, Zorica
AU  - Dimitrijević, Miroslava
AU  - Čolić, Miodrag
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/501
AB  - We designed the present study to provide evidence of the immunomodulatory effects of pentoxifylline (PTX) in experimental autoimmune myocarditis (EAM) in rats. PTX is xantine-derived agent known to inhibit the production of TNF-α and his beneficial effects have been reported in patients with dilated cardiomyopathy. In this study we examined the efficacy of PTX in the treatment of experimental autoimmune myocarditis as a paradigm of the autoimmune mechanisms involved in pathogenesis of dilated cardiomiopathy. Male DA rats immunized with porcine cardiac myosin were treated i.m. with PTX (200 mg/kg/day) over 7 days, begenning either on the day of immunization (early treatment group), or on day 14. postimmunization (late treatment group). Disease course and severity were evaluated by macroscopic score of the heart, heart weight/body weight ratio (Hw/Bw), histological and immunohistochemical analysis of cardiac tisssue. We found in our study that PTX exhibited both preventive and therapeutical effects in EAM.
AB  - U radu je ispitivan uticaj pentoksifilina (PTX) na razvoj i tok eksperimentalnog autoimunog miokarditisa pacova. Pentoksifilin je derivat ksantina sa inhibitornim efektom na produkciju TNF-α, a klinički je potvrđen i njegov pozitivan učinak u terapiji pacijenata sa dilatacionom kardiomiopatijom. U ispitivanju je testiran imunomodulatorni efekat PTX u tretmanu eksperimentalnog autoimunog miokarditisa koji predstavlja eksperimentalni model za proučavanje autoimunskih mehanizama uključenih u razvoj dilatacione kardiomiopatije. Pacovi DA soja imunizovani srčanim miozinom tretirani su pentoksifilinom u dozi od 200 mg/kg t.m., počev od 0.-6. dana (rani tretman), ili od 14.-20. dana (kasni tretman) u odnosu na termin imunizacije miozinom. Razvoj i intenzitet miokarditisa praćeni su analizom makroskopskih karakteristika srca, indeksa masa srca/telesna masa (Hwt/Bwt), histoloških i imunohistohemijskih analiza miokarda. Dobijeni rezultati su pokazali da PTX ispoljava profilaktičan i terapijski učinak u eksperimentalnom autoimunom miokarditisu.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Immunomodulation of experimental autoimmune myocarditis by pentoxifylline
T1  - Autoimunskog miokarditisa primenom pentoksifilina
VL  - 54
IS  - 6
SP  - 761
EP  - 771
UR  - https://hdl.handle.net/21.15107/rcub_farfar_501
ER  - 

@article{
author = "Milenković, Marina and Milosavljević, Petar and Stojić-Vukanić, Zorica and Dimitrijević, Miroslava and Čolić, Miodrag",
year = "2004",
abstract = "We designed the present study to provide evidence of the immunomodulatory effects of pentoxifylline (PTX) in experimental autoimmune myocarditis (EAM) in rats. PTX is xantine-derived agent known to inhibit the production of TNF-α and his beneficial effects have been reported in patients with dilated cardiomyopathy. In this study we examined the efficacy of PTX in the treatment of experimental autoimmune myocarditis as a paradigm of the autoimmune mechanisms involved in pathogenesis of dilated cardiomiopathy. Male DA rats immunized with porcine cardiac myosin were treated i.m. with PTX (200 mg/kg/day) over 7 days, begenning either on the day of immunization (early treatment group), or on day 14. postimmunization (late treatment group). Disease course and severity were evaluated by macroscopic score of the heart, heart weight/body weight ratio (Hw/Bw), histological and immunohistochemical analysis of cardiac tisssue. We found in our study that PTX exhibited both preventive and therapeutical effects in EAM., U radu je ispitivan uticaj pentoksifilina (PTX) na razvoj i tok eksperimentalnog autoimunog miokarditisa pacova. Pentoksifilin je derivat ksantina sa inhibitornim efektom na produkciju TNF-α, a klinički je potvrđen i njegov pozitivan učinak u terapiji pacijenata sa dilatacionom kardiomiopatijom. U ispitivanju je testiran imunomodulatorni efekat PTX u tretmanu eksperimentalnog autoimunog miokarditisa koji predstavlja eksperimentalni model za proučavanje autoimunskih mehanizama uključenih u razvoj dilatacione kardiomiopatije. Pacovi DA soja imunizovani srčanim miozinom tretirani su pentoksifilinom u dozi od 200 mg/kg t.m., počev od 0.-6. dana (rani tretman), ili od 14.-20. dana (kasni tretman) u odnosu na termin imunizacije miozinom. Razvoj i intenzitet miokarditisa praćeni su analizom makroskopskih karakteristika srca, indeksa masa srca/telesna masa (Hwt/Bwt), histoloških i imunohistohemijskih analiza miokarda. Dobijeni rezultati su pokazali da PTX ispoljava profilaktičan i terapijski učinak u eksperimentalnom autoimunom miokarditisu.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Immunomodulation of experimental autoimmune myocarditis by pentoxifylline, Autoimunskog miokarditisa primenom pentoksifilina",
volume = "54",
number = "6",
pages = "761-771",
url = "https://hdl.handle.net/21.15107/rcub_farfar_501"
}

Milenković, M., Milosavljević, P., Stojić-Vukanić, Z., Dimitrijević, M.,& Čolić, M.. (2004). Immunomodulation of experimental autoimmune myocarditis by pentoxifylline. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 54(6), 761-771.
https://hdl.handle.net/21.15107/rcub_farfar_501

Milenković M, Milosavljević P, Stojić-Vukanić Z, Dimitrijević M, Čolić M. Immunomodulation of experimental autoimmune myocarditis by pentoxifylline. in Arhiv za farmaciju. 2004;54(6):761-771.
https://hdl.handle.net/21.15107/rcub_farfar_501 .

Milenković, Marina, Milosavljević, Petar, Stojić-Vukanić, Zorica, Dimitrijević, Miroslava, Čolić, Miodrag, "Immunomodulation of experimental autoimmune myocarditis by pentoxifylline" in Arhiv za farmaciju, 54, no. 6 (2004):761-771,
https://hdl.handle.net/21.15107/rcub_farfar_501 .

TY  - JOUR
AU  - Čolić, Miodrag
AU  - Jandrić, Dušan
AU  - Stojić-Vukanić, Zorica
AU  - Antić-Stanković, Jelena
AU  - Popović, Petar
AU  - Vasilijić, Saša
AU  - Milosavljević, Petar
AU  - Balint, Bela
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/465
AB  - Several laboratories have developed culture systems that allow the generation of large numbers of human dendritic cells (DC) from monocytes using granulocyte-macrophage colony stimulating factor (GM-CSF), and interleukin-4 (IL-4). In this work we provided evidence that GM-CSF (100 ng/ml) in combination with a low concentration of IL-4 (5 ng/ml) was efficient in the generation of immature, non-adherent, monocyte-derived DC as the same concentration of GM-CSF, and ten times higher concentration of IL-4 (50 ng/ml). This conclusion was based on the similar phenotype profile of DC such as the expression of CD1a, CD80, CD86, and HLA-DR, down-regulation of CD14, and the absence of CD83, as well as on their similar allostimulatory activity for T cells. A higher number of cells remained adherent in cultures with lower concentrations of IL-4 than in cultures with higher concentrations of the cytokine. However, most of these adherent cells down-regulated CD14 and stimulated the proliferation of alloreactive T cells. In contrast adherent cells cultivated with GM-CSF alone were predominantly macrophages as judged by the expression of CD14 and the inefficiency to stimulate alloreactive T cells. DC generated in the presence of lower concentrations of IL-4 had higher proapoptotic potential for the Jurkat cell line than DC differentiated with higher concentrations of IL-4, suggesting their stronger cytotoxic, anti-tumor effect.
AB  - U više laboratorija su uspostavljeni sistemi za kultivaciju velikog broja humanih dendritičnih ćelija (DĆ) od monocita korišćenjem faktora stimulacije granulocitno--makrofagnih kolonija (GM-CSF) i interleukina-4 (IL-4). U ovom radu je pokazano da je kombinacija GM-CSF (100 ng/ml) i mala koncentracija IL-4 (5 ng/ml) podjednako efikasna za dobijanje nezrelih, neadherentnih, DĆ monocitnog porekla kao i kombinacija GM-CSF sa deset puta većom koncentracijom IL-4 (50 ng/ml). Ovaj zaključak izveden je na osnovu sličnog fenotipskog profila DĆ (ispoljavanje CD1a, CD80, CD86 i HLA-DR, smanjenje ekspresije CD14 i odsustva CD83), kao i slične alostimulatorne aktivnosti ovih ćelija za limfocite T. U kulturama sa nižim koncentracijama IL-4 prisutan je bio veći broj adherentnih ćelija nego u kulturama sa većim koncentracijama IL-4. Međutim, većina ovih ćelija je smanjivala ekspresiju CD14 i stimulisala proliferaciju aloreaktivnih limfocita T. Nasuprot njima adherentne ćelije, diferentovane samo u prisustvu GM-CSF, koje su ispoljavale CD14 i nisu imale sposobnost stimulacije aloreakativnih limfocita T pokazivale su karakteristike makrofaga. DĆ obrazovane u prisustvu manjih koncentracija IL-4 imale su veći potencijal za indukciju apoptoze Jurkat ćelijske linije, a time i snažniji citotoksični, antitumorski efekat nego DĆ diferentovane u prisustvu većih koncentracija IL-4.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4
T1  - Diferencijacija humanih dendritičnih ćelija od monocita in vitro korišćenjem faktora stimulacije granulocitno-makrofagnih kolonija i niske koncentracije interleukina-4
VL  - 60
IS  - 5
SP  - 531
EP  - 538
DO  - 10.2298/VSP0305531C
ER  - 

@article{
author = "Čolić, Miodrag and Jandrić, Dušan and Stojić-Vukanić, Zorica and Antić-Stanković, Jelena and Popović, Petar and Vasilijić, Saša and Milosavljević, Petar and Balint, Bela",
year = "2003",
abstract = "Several laboratories have developed culture systems that allow the generation of large numbers of human dendritic cells (DC) from monocytes using granulocyte-macrophage colony stimulating factor (GM-CSF), and interleukin-4 (IL-4). In this work we provided evidence that GM-CSF (100 ng/ml) in combination with a low concentration of IL-4 (5 ng/ml) was efficient in the generation of immature, non-adherent, monocyte-derived DC as the same concentration of GM-CSF, and ten times higher concentration of IL-4 (50 ng/ml). This conclusion was based on the similar phenotype profile of DC such as the expression of CD1a, CD80, CD86, and HLA-DR, down-regulation of CD14, and the absence of CD83, as well as on their similar allostimulatory activity for T cells. A higher number of cells remained adherent in cultures with lower concentrations of IL-4 than in cultures with higher concentrations of the cytokine. However, most of these adherent cells down-regulated CD14 and stimulated the proliferation of alloreactive T cells. In contrast adherent cells cultivated with GM-CSF alone were predominantly macrophages as judged by the expression of CD14 and the inefficiency to stimulate alloreactive T cells. DC generated in the presence of lower concentrations of IL-4 had higher proapoptotic potential for the Jurkat cell line than DC differentiated with higher concentrations of IL-4, suggesting their stronger cytotoxic, anti-tumor effect., U više laboratorija su uspostavljeni sistemi za kultivaciju velikog broja humanih dendritičnih ćelija (DĆ) od monocita korišćenjem faktora stimulacije granulocitno--makrofagnih kolonija (GM-CSF) i interleukina-4 (IL-4). U ovom radu je pokazano da je kombinacija GM-CSF (100 ng/ml) i mala koncentracija IL-4 (5 ng/ml) podjednako efikasna za dobijanje nezrelih, neadherentnih, DĆ monocitnog porekla kao i kombinacija GM-CSF sa deset puta većom koncentracijom IL-4 (50 ng/ml). Ovaj zaključak izveden je na osnovu sličnog fenotipskog profila DĆ (ispoljavanje CD1a, CD80, CD86 i HLA-DR, smanjenje ekspresije CD14 i odsustva CD83), kao i slične alostimulatorne aktivnosti ovih ćelija za limfocite T. U kulturama sa nižim koncentracijama IL-4 prisutan je bio veći broj adherentnih ćelija nego u kulturama sa većim koncentracijama IL-4. Međutim, većina ovih ćelija je smanjivala ekspresiju CD14 i stimulisala proliferaciju aloreaktivnih limfocita T. Nasuprot njima adherentne ćelije, diferentovane samo u prisustvu GM-CSF, koje su ispoljavale CD14 i nisu imale sposobnost stimulacije aloreakativnih limfocita T pokazivale su karakteristike makrofaga. DĆ obrazovane u prisustvu manjih koncentracija IL-4 imale su veći potencijal za indukciju apoptoze Jurkat ćelijske linije, a time i snažniji citotoksični, antitumorski efekat nego DĆ diferentovane u prisustvu većih koncentracija IL-4.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4, Diferencijacija humanih dendritičnih ćelija od monocita in vitro korišćenjem faktora stimulacije granulocitno-makrofagnih kolonija i niske koncentracije interleukina-4",
volume = "60",
number = "5",
pages = "531-538",
doi = "10.2298/VSP0305531C"
}

Čolić, M., Jandrić, D., Stojić-Vukanić, Z., Antić-Stanković, J., Popović, P., Vasilijić, S., Milosavljević, P.,& Balint, B.. (2003). Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 60(5), 531-538.
https://doi.org/10.2298/VSP0305531C

Čolić M, Jandrić D, Stojić-Vukanić Z, Antić-Stanković J, Popović P, Vasilijić S, Milosavljević P, Balint B. Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4. in Vojnosanitetski pregled. 2003;60(5):531-538.
doi:10.2298/VSP0305531C .

Čolić, Miodrag, Jandrić, Dušan, Stojić-Vukanić, Zorica, Antić-Stanković, Jelena, Popović, Petar, Vasilijić, Saša, Milosavljević, Petar, Balint, Bela, "Differentiation of human dendritic cells from monocytes in vitro using granulocyte-macrophage colony stimulating factor and low concentration of interleukin-4" in Vojnosanitetski pregled, 60, no. 5 (2003):531-538,
https://doi.org/10.2298/VSP0305531C . .