@article{
author = "Minić, Rajna and Arsić, Aleksandra and Kojadinović, Milica and Palibrk, Aleksa and Đorđević, Brižita and Stević, Zorica",
year = "2023",
abstract = "Background: Recent literature data highlights metabolic changes in amyotrophic lateral sclerosis (ALS). To explore possible early metabolic changes, we aimed to analyse the fatty acids (FA) composition of erythrocytes in newly diagnosed ALS patients and to see whether fatty acid levels correlate with the ALSFRS-R score or disease duration. Methods: The severity of motor function involvement was assessed by the ALSFRS-R scale at the initial evaluation. The fatty acid profile of erythrocyte membranes was analysed by gas-liquid chromatography. The study comprised 26 clinically diagnosed ALS patients, with mean ALSFRS-R 38±8. The control group included 26 healthy volunteers. Results: Significantly higher levels of palmitic acid and total saturated FAs were detected in ALS patients. In ALS patients, total monounsaturated FA, palmitoleic, vaccenic, and oleic acid were also significantly increased. The levels of eicosapentaenoic acid, docosapentaenoic acid, total polyunsaturated FA (PUFA) and n-6 PUFA were significantly lower in ALS patients. Additionally, a-linolenic acid, the precursor of the n-3 PUFA family, was not detected in ALS patients. We found no significant correlation between the ALSFRS-R score and the abundance of individual FAs analysed. A moderate negative correlation was found between disease duration and DHA level, and a positive correlation was detected with MUFA. Conclusion: Experimental evidence presented may contribute to shaping a beneficial nutritional intervention., Uvod: Dostupni literaturni podaci ističu značaj metaboličkih promena kod amiotrofične lateralne skleroze (ALS). Sa ciljem da istražimo moguće rane metaboličke promene analizirali smo sastav masnih kiselina (MK) eritrocita kod novodijagnostikovanih ALS pacijenata da bi videli da li nivoi masnih kiselina koreliraju sa ALSFRS-R ocenom ili trajanjem bolesti. Metode: Stepen oštećenja motoričke funkcije procenjivan je ALSFRS-R skalom pri početnoj proceni. Studija je obuhvatila 26 klinički dijagnostikovanih ALS pacijenata, srednja vrednost ALSFRS-R bila je 38±8. Kontrolnu grupu činilo je 26 zdravih dobrovoljaca. Profil masnih kiselina membrana eritrocita je analiziran gasno-tečnom hromatografijom. Rezultati: Značajno viši nivoi palmitinske kiseline i ukupnih zasićenih MK nađeni su kod ALS pacijenata. Ukupne mono nezasićene MK, palmitoleinska, vakcenska i oleinska kiselina takođe su bile značajno povećane kod ALS pacijenata. Nivoi eikozapentaenske kiseline, dokozapentaenske kiseline, ukupne polinezasićene MK (PUFA) i n-6 PUFA bili su značajno niži kod pacijenata sa ALS. Pored toga, a- linolenska kiselina, prekursor porodice n-3 PUFA, uopšte nije detektovana kod ALS pacijenata. Nismo prona{li zna- ~ajnu korelaciju izme|u ALSFRS-R ocene i nivoa pojedinačnih analiziranih MK. Umerena negativna korelacija je prona|ena između trajanja bolesti i nivoa DHA, a nađena je pozitivna korelacija trajanja bolesti sa nivoom MUFA. Zaključak: Prikazani eksperimentalni dokazi mogu doprineti oblikovanju korisne nutritivne intervencije.",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Erythrocyte fatty acid aberrations in amyotrophic lateral sclerosis – correlation with disease duration, Izmenjen profil masnih kiselina eritrocita pacijenata sa amiotrofičnom lateralnom sklerozom – korelacija sa trajanjem bolesti",
volume = "42",
number = "4",
pages = "621-629",
doi = "10.5937/jomb0-40387"
}
