TY  - JOUR
AU  - Kozarević Cilović, Emina
AU  - Dautović, Esmeralda
AU  - Halilčević, Dalila
AU  - Softić, Adaleta
AU  - Srabović, Nahida
AU  - Šarić-Kundalić, Broza
AU  - Delić, Nina
AU  - Kolarević, Lamija
AU  - Mekić, Lejla
AU  - Ibišević, Merima
AU  - Horozić, Emir
AU  - Arsenijević, Jelena
AU  - Maksimović, Zoran
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4624
AB  - Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer.

Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay.

Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxidant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts.

Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro.
PB  - International Research Journal of Pure and Applied Chemistry
T2  - International Research Journal of Pure and Applied Chemistry
T1  - Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts
VL  - 23
IS  - 5
SP  - 23
EP  - 32
DO  - 10.9734/irjpac/2022/v23i5788
ER  - 

@article{
author = "Kozarević Cilović, Emina and Dautović, Esmeralda and Halilčević, Dalila and Softić, Adaleta and Srabović, Nahida and Šarić-Kundalić, Broza and Delić, Nina and Kolarević, Lamija and Mekić, Lejla and Ibišević, Merima and Horozić, Emir and Arsenijević, Jelena and Maksimović, Zoran",
year = "2022",
abstract = "Aims: The aims of the study were to analyse the polyphenols of Pulicaria dysenterica (L.) Bernh. methanolic extracts from aerial and underground parts, assessment of antioxidant activity and to evaluate their cytotoxicity on HeLa cells of cervical cancer.

Methodology: The total phenolic content (TPC) of extracts was determined by the Folin-Ciocalteu spectrophotometric method. The qualitative and quantitative analysis of individual polyphenolic compounds were performed by the reverse phase HPLC method. The antioxidant capacity was evaluated by both, 2,2-diphenyl1-picrylhydrazyl radical and FRAP assay, while cytotoxicity of the extracts was assessed by MTT assay.

Results: TPC of the samples were 127.62±2.22 and 244.12±8.84 mg gallic acid equivalent/g extract. In the extracts chlorogenic acid in amount of 10.06±0.96 and 11.32±0.28 mg/g, flavonoid rutin in amount of 5.68±0.13 mg/g and three caffeic acid derivatives were recorded. Extract from underground parts achieved better antioxidant activity with IC50 value 55.36±0.75 µg/mL and FRAP value 2411.12±37.22 µmol Fe2+g-1 compared to the one from aerial parts. Extract from aerial parts achieved better cytotoxic activity with 50% inhibition of viability (IC50) at concentration of 0.389±0.07 mg/mL, against HeLa cells, compared to the extract from underground parts.

Conclusion: Analyzed Pulicaria dysenterica extracts contained phenolic acids and flavonoids. The extracts showed good antioxidant activity and cytotoxic properties against HeLa cells in vitro.",
publisher = "International Research Journal of Pure and Applied Chemistry",
journal = "International Research Journal of Pure and Applied Chemistry",
title = "Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts",
volume = "23",
number = "5",
pages = "23-32",
doi = "10.9734/irjpac/2022/v23i5788"
}

Kozarević Cilović, E., Dautović, E., Halilčević, D., Softić, A., Srabović, N., Šarić-Kundalić, B., Delić, N., Kolarević, L., Mekić, L., Ibišević, M., Horozić, E., Arsenijević, J.,& Maksimović, Z.. (2022). Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts. in International Research Journal of Pure and Applied Chemistry
International Research Journal of Pure and Applied Chemistry., 23(5), 23-32.
https://doi.org/10.9734/irjpac/2022/v23i5788

Kozarević Cilović E, Dautović E, Halilčević D, Softić A, Srabović N, Šarić-Kundalić B, Delić N, Kolarević L, Mekić L, Ibišević M, Horozić E, Arsenijević J, Maksimović Z. Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts. in International Research Journal of Pure and Applied Chemistry. 2022;23(5):23-32.
doi:10.9734/irjpac/2022/v23i5788 .

Kozarević Cilović, Emina, Dautović, Esmeralda, Halilčević, Dalila, Softić, Adaleta, Srabović, Nahida, Šarić-Kundalić, Broza, Delić, Nina, Kolarević, Lamija, Mekić, Lejla, Ibišević, Merima, Horozić, Emir, Arsenijević, Jelena, Maksimović, Zoran, "Antioxidant and Cytotoxic Activities of Pulicaria dysenterica Methanol Extracts" in International Research Journal of Pure and Applied Chemistry, 23, no. 5 (2022):23-32,
https://doi.org/10.9734/irjpac/2022/v23i5788 . .