TY  - CONF
AU  - Vidović, Bojana
AU  - Guerreiro, I.
AU  - Costa, J.G.
AU  - Oliveira, N.G.
AU  - Saraiva, N.
AU  - Fernandes, A.S.
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5396
AB  - A large number of epidemiological studies have linked cruciferous vegetable consumption to
a reduced risk of various types of cancer, including renal cell carcinoma. Organosulfur
compounds from cruciferous vegetables, glucosinolates and their metabolic breakdown
derivatives, isothiocyanates and indole-3-carbinol, rise scientific interest by exerting unique
anticancer properties. Erucin (ER) is an isothiocyanate that is generated by enzymatic
hydrolyzes of glucoerucin, a glucosinolate predominant found in rocket species, or by in vivo
reduction of sulforaphane, its structural oxidized analog. In the present study, the inhibitory
effects of ER on renal cancer cell viability, migration and invasion were investigated. The 786-
O human renal cancer cell line and the Vero normal-like cells were treated with different
concentrations of ER (10-100 μM). Cell viability was determined using the MTT and PI assays.
The intracellular level of reactive oxygen species was evaluated using
dichlorodihydrofluorescein diacetate. The collective cell migration and
chemotaxis/chemoinvasion were studied by a wound healing and transwell assay, respectively.
ER induced a concentration-dependent decrease in cell viability, with more cytotoxicity for
786-O cells than against Vero cells. Non-cytotoxic concentration of ER significantly reduced
cell migration rate, chemotaxis and invasiveness potential of 786-O cells. The observed
favorable anticancer potential of ER against human renal carcinoma in vitro requires further
investigation.
PB  - University of Belgrade, Belgrade, Serbia
C3  - Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade
T1  - Is erucin a promising bioactive against renal cell carcinoma?
SP  - 30
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5396
ER  - 

@conference{
author = "Vidović, Bojana and Guerreiro, I. and Costa, J.G. and Oliveira, N.G. and Saraiva, N. and Fernandes, A.S.",
year = "2020",
abstract = "A large number of epidemiological studies have linked cruciferous vegetable consumption to
a reduced risk of various types of cancer, including renal cell carcinoma. Organosulfur
compounds from cruciferous vegetables, glucosinolates and their metabolic breakdown
derivatives, isothiocyanates and indole-3-carbinol, rise scientific interest by exerting unique
anticancer properties. Erucin (ER) is an isothiocyanate that is generated by enzymatic
hydrolyzes of glucoerucin, a glucosinolate predominant found in rocket species, or by in vivo
reduction of sulforaphane, its structural oxidized analog. In the present study, the inhibitory
effects of ER on renal cancer cell viability, migration and invasion were investigated. The 786-
O human renal cancer cell line and the Vero normal-like cells were treated with different
concentrations of ER (10-100 μM). Cell viability was determined using the MTT and PI assays.
The intracellular level of reactive oxygen species was evaluated using
dichlorodihydrofluorescein diacetate. The collective cell migration and
chemotaxis/chemoinvasion were studied by a wound healing and transwell assay, respectively.
ER induced a concentration-dependent decrease in cell viability, with more cytotoxicity for
786-O cells than against Vero cells. Non-cytotoxic concentration of ER significantly reduced
cell migration rate, chemotaxis and invasiveness potential of 786-O cells. The observed
favorable anticancer potential of ER against human renal carcinoma in vitro requires further
investigation.",
publisher = "University of Belgrade, Belgrade, Serbia",
journal = "Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade",
title = "Is erucin a promising bioactive against renal cell carcinoma?",
pages = "30-30",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5396"
}

Vidović, B., Guerreiro, I., Costa, J.G., Oliveira, N.G., Saraiva, N.,& Fernandes, A.S.. (2020). Is erucin a promising bioactive against renal cell carcinoma?. in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade
University of Belgrade, Belgrade, Serbia., 30-30.
https://hdl.handle.net/21.15107/rcub_farfar_5396

Vidović B, Guerreiro I, Costa J, Oliveira N, Saraiva N, Fernandes A. Is erucin a promising bioactive against renal cell carcinoma?. in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade. 2020;:30-30.
https://hdl.handle.net/21.15107/rcub_farfar_5396 .

Vidović, Bojana, Guerreiro, I., Costa, J.G., Oliveira, N.G., Saraiva, N., Fernandes, A.S., "Is erucin a promising bioactive against renal cell carcinoma?" in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade (2020):30-30,
https://hdl.handle.net/21.15107/rcub_farfar_5396 .