TY  - JOUR
AU  - Stojić-Vukanić, Zorica
AU  - Čolić, Miodrag
AU  - Backović, A.
AU  - Antić-Stanković, Jelena
AU  - Bufan, Biljana
AU  - Dimitrijević, M.
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1580
AB  - Leflunomide is an immunosuppressive drug effective in experimental models of transplantation and autoimmune diseases and in the treatment of active rheumatoid arthritis (RA). Having in mind that it has been shown that some other immunosuppressive drugs (glucocorticoids, mycophenolate mofetil, sirolimus etc.) impair dendritic cell (DC) phenotype and function, we investigated the effect of A77 1726, an active metabolite of leflunomide, on the differentiation and function of human monocyte-derived dendritic cells (MDDC) in vitro. Immature MDDC were generated by cultivating monocytes in medium supplemented with GM-CSF and IL-4. To induce maturation, immature MDDC were cultured for 2 additional days with LPS. A77 1726 (100 μM) was added at the beginning of cultivation. Flow cytometric analysis showed that MDDC differentiated in the presence of A77 1726 exhibited an altered phenotype, with a down-regulated surface expression of CD80, CD86, CD54 and CD40 molecules. Furthermore, the continuous presence of A77 1726 during differentiation and maturation prevented successful maturation, judging by the decreased expression of maturation marker CD83, costimulatory and adhesive molecules on A77 1726-treated mature MDDC. In addition, A77 1726-pretreated MDDC exhibited a poor stimulatory capacity of the allogeneic T cells and a low production of IL-10 and IL-18. These data suggest that leflunomide impairs the differentiation, maturation and function of human MDDC in vitro, which is an additional mechanism of its immunosuppressive effect.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Differentiation and function of human monocyte-derived dendritic cells under the influence of leflunomide
VL  - 63
IS  - 2
SP  - 353
EP  - 364
DO  - 10.2298/ABS1102353S
ER  - 

@article{
author = "Stojić-Vukanić, Zorica and Čolić, Miodrag and Backović, A. and Antić-Stanković, Jelena and Bufan, Biljana and Dimitrijević, M.",
year = "2011",
abstract = "Leflunomide is an immunosuppressive drug effective in experimental models of transplantation and autoimmune diseases and in the treatment of active rheumatoid arthritis (RA). Having in mind that it has been shown that some other immunosuppressive drugs (glucocorticoids, mycophenolate mofetil, sirolimus etc.) impair dendritic cell (DC) phenotype and function, we investigated the effect of A77 1726, an active metabolite of leflunomide, on the differentiation and function of human monocyte-derived dendritic cells (MDDC) in vitro. Immature MDDC were generated by cultivating monocytes in medium supplemented with GM-CSF and IL-4. To induce maturation, immature MDDC were cultured for 2 additional days with LPS. A77 1726 (100 μM) was added at the beginning of cultivation. Flow cytometric analysis showed that MDDC differentiated in the presence of A77 1726 exhibited an altered phenotype, with a down-regulated surface expression of CD80, CD86, CD54 and CD40 molecules. Furthermore, the continuous presence of A77 1726 during differentiation and maturation prevented successful maturation, judging by the decreased expression of maturation marker CD83, costimulatory and adhesive molecules on A77 1726-treated mature MDDC. In addition, A77 1726-pretreated MDDC exhibited a poor stimulatory capacity of the allogeneic T cells and a low production of IL-10 and IL-18. These data suggest that leflunomide impairs the differentiation, maturation and function of human MDDC in vitro, which is an additional mechanism of its immunosuppressive effect.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Differentiation and function of human monocyte-derived dendritic cells under the influence of leflunomide",
volume = "63",
number = "2",
pages = "353-364",
doi = "10.2298/ABS1102353S"
}

Stojić-Vukanić, Z., Čolić, M., Backović, A., Antić-Stanković, J., Bufan, B.,& Dimitrijević, M.. (2011). Differentiation and function of human monocyte-derived dendritic cells under the influence of leflunomide. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(2), 353-364.
https://doi.org/10.2298/ABS1102353S

Stojić-Vukanić Z, Čolić M, Backović A, Antić-Stanković J, Bufan B, Dimitrijević M. Differentiation and function of human monocyte-derived dendritic cells under the influence of leflunomide. in Archives of Biological Sciences. 2011;63(2):353-364.
doi:10.2298/ABS1102353S .

Stojić-Vukanić, Zorica, Čolić, Miodrag, Backović, A., Antić-Stanković, Jelena, Bufan, Biljana, Dimitrijević, M., "Differentiation and function of human monocyte-derived dendritic cells under the influence of leflunomide" in Archives of Biological Sciences, 63, no. 2 (2011):353-364,
https://doi.org/10.2298/ABS1102353S . .

TY  - JOUR
AU  - Colić, M.
AU  - Došlov-Kokoruš, Zvezdana
AU  - Backović, A.
AU  - Stojanović, D.
AU  - Antić-Stanković, Jelena
AU  - Kovačević, Nada
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/989
AB  - The effect of the methanolic extract of the underground parts of Epimedium alpinum L. (MEEA) on the immune response to Keyhole Limpet Hemocyanine (KLH) or alloantigens in vivo was studied in AO rats. Immunization of experimental animals with KLH or allogeneic lymphocytes together with MEEA was followed by an increase in cellularity of draining lymph nodes (LN) and enhanced proliferation of LN lymphocytes after their restimulation with specific antigens in vitro, compared to control rats immunized without MEEA. These effects correlated with an increase in relative values of B, MHC class II+, CD25(+) and CD71(+) cells, whereas percentages of T cells and both subsets of T cells (CD4(+) and CD8(+)) were not significantly altered. As a consequence of higher LN cellularity, total numbers of all cell subsets in the MEEA-treated group of rats were significantly increased, compared to the corresponding control. The addition of MEEA together with KLH in vitro to LN lymphocytes of rats immunized with KLH or KLH and MEEA in vivo was manifested by significant increase (0.1 mu g/ml of MEEA) and decrease (50 mu g/ml and 100 mu g/ml of MEEA) of cell proliferation, respectively. However, when LN lymphocytes from rats, immunized in vivo with KLH and MEEA, were stimulated in vitro with MEEA together with an anti-alpha beta T cell receptor monoclonal antibody (R73), their proliferation was siginificantly inhibited. Taken together, obtained results suggest that MEEA possesses immunostimulatory activity in vivo, but some components from the extract exert immunosuppressive effects in vitro on previously in vivo activated T cells.
PB  - Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn
T2  - Pharmazie
T1  - Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo
VL  - 62
IS  - 9
SP  - 705
EP  - 708
UR  - https://hdl.handle.net/21.15107/rcub_farfar_989
ER  - 

@article{
author = "Colić, M. and Došlov-Kokoruš, Zvezdana and Backović, A. and Stojanović, D. and Antić-Stanković, Jelena and Kovačević, Nada",
year = "2007",
abstract = "The effect of the methanolic extract of the underground parts of Epimedium alpinum L. (MEEA) on the immune response to Keyhole Limpet Hemocyanine (KLH) or alloantigens in vivo was studied in AO rats. Immunization of experimental animals with KLH or allogeneic lymphocytes together with MEEA was followed by an increase in cellularity of draining lymph nodes (LN) and enhanced proliferation of LN lymphocytes after their restimulation with specific antigens in vitro, compared to control rats immunized without MEEA. These effects correlated with an increase in relative values of B, MHC class II+, CD25(+) and CD71(+) cells, whereas percentages of T cells and both subsets of T cells (CD4(+) and CD8(+)) were not significantly altered. As a consequence of higher LN cellularity, total numbers of all cell subsets in the MEEA-treated group of rats were significantly increased, compared to the corresponding control. The addition of MEEA together with KLH in vitro to LN lymphocytes of rats immunized with KLH or KLH and MEEA in vivo was manifested by significant increase (0.1 mu g/ml of MEEA) and decrease (50 mu g/ml and 100 mu g/ml of MEEA) of cell proliferation, respectively. However, when LN lymphocytes from rats, immunized in vivo with KLH and MEEA, were stimulated in vitro with MEEA together with an anti-alpha beta T cell receptor monoclonal antibody (R73), their proliferation was siginificantly inhibited. Taken together, obtained results suggest that MEEA possesses immunostimulatory activity in vivo, but some components from the extract exert immunosuppressive effects in vitro on previously in vivo activated T cells.",
publisher = "Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn",
journal = "Pharmazie",
title = "Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo",
volume = "62",
number = "9",
pages = "705-708",
url = "https://hdl.handle.net/21.15107/rcub_farfar_989"
}

Colić, M., Došlov-Kokoruš, Z., Backović, A., Stojanović, D., Antić-Stanković, J.,& Kovačević, N.. (2007). Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo. in Pharmazie
Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn., 62(9), 705-708.
https://hdl.handle.net/21.15107/rcub_farfar_989

Colić M, Došlov-Kokoruš Z, Backović A, Stojanović D, Antić-Stanković J, Kovačević N. Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo. in Pharmazie. 2007;62(9):705-708.
https://hdl.handle.net/21.15107/rcub_farfar_989 .

Colić, M., Došlov-Kokoruš, Zvezdana, Backović, A., Stojanović, D., Antić-Stanković, Jelena, Kovačević, Nada, "Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo" in Pharmazie, 62, no. 9 (2007):705-708,
https://hdl.handle.net/21.15107/rcub_farfar_989 .

TY  - CONF
AU  - Stojić-Vukanić, Zorica
AU  - Čolić, Miodrag
AU  - Backović, A.
AU  - Antić-Stanković, Jelena
AU  - Dimitrijević, M.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/684
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro
T1  - In vitro efekat pentoksifilina na diferencijaciju i maturaciju humanih dendritičnih ćelija monocitnog porekla
VL  - 56
IS  - 4
SP  - 410
EP  - 411
UR  - https://hdl.handle.net/21.15107/rcub_farfar_684
ER  - 

@conference{
author = "Stojić-Vukanić, Zorica and Čolić, Miodrag and Backović, A. and Antić-Stanković, Jelena and Dimitrijević, M.",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro, In vitro efekat pentoksifilina na diferencijaciju i maturaciju humanih dendritičnih ćelija monocitnog porekla",
volume = "56",
number = "4",
pages = "410-411",
url = "https://hdl.handle.net/21.15107/rcub_farfar_684"
}

Stojić-Vukanić, Z., Čolić, M., Backović, A., Antić-Stanković, J.,& Dimitrijević, M.. (2006). Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(4), 410-411.
https://hdl.handle.net/21.15107/rcub_farfar_684

Stojić-Vukanić Z, Čolić M, Backović A, Antić-Stanković J, Dimitrijević M. Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro. in Arhiv za farmaciju. 2006;56(4):410-411.
https://hdl.handle.net/21.15107/rcub_farfar_684 .

Stojić-Vukanić, Zorica, Čolić, Miodrag, Backović, A., Antić-Stanković, Jelena, Dimitrijević, M., "Effect of pentoxifylline on differentiation and maturation of human monocyte-derived dendritic cells in vitro" in Arhiv za farmaciju, 56, no. 4 (2006):410-411,
https://hdl.handle.net/21.15107/rcub_farfar_684 .