TY  - JOUR
AU  - Ignjatović, Janko
AU  - Đajić, Nevena
AU  - Krmar, Jovana
AU  - Protić, Ana
AU  - Štrukelj, Borut
AU  - Otašević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3797
AB  - Recently, growing interest has been devoted to the investigation of compounds with antimicrobial activity due to rising cases of resistance of mic-robes to known therapies. A reliable and versatile source of novel drug disco-very was recently found among endophytic fungi. Hitherto, the research usu-ally  enclosed  the in  vitro  evaluation  of  antimicrobial  activity  and  chemical structure  elucidation  of  biomolecules  extracted  from  fungal  material.  There-fore, this research was designed as an extension to previous investigations of endophytic fungi growing on conifer needles by means of conducting a mole-cular docking study. The in  silico methods were used with the main goal to make a contribution to the understanding of the  mechanisms underlying the interaction of biomolecules isolated from fungus Phomopsis  species and eight different types of receptors that belong to usually multidrug resistant bacterial pathogens.  The  results  revealed  valuable  interactions  with  receptors  3G7B (Staphylococcus  aureus’s  gyrase  B),  1F0K  (1.9  Å  structure  of Escherichia coli’s  transferase)  and  1SHV  (Klebsiella  pneumoniae’s  SHV-1 β-lactamase) thus pointing out the receptors that trigger antibiotic response upon activation by  the  most  potent  compounds  325-3,  325-5,  phomoenamide  and  phomol. These findings also recommended further discovery of novel potent and broad-spectrum antibiotics based on the structure of selected molecules.
AB  - У последње време, као одговорна повећање резистенције микроорганизама на познату терапију, све већа пажња се поклања истраживању једињења са антимикробном активношћу. Ендофитне гљиве су недавно представљене као поуздан и богат извор за развој нових лекова. До сада, истраживања су се углавном ограничавала на in vitro процену антимикробне активности и разоткривање хемијске структуре биомолекула изолованих из материјала гљива. Из тог разлога, ово истраживање је осмишљено као проширење претходно спроведених испитивања ендофита које расту на иглицама четинара путем in silico студије молекулског докинга. Главни циљ употребе in silico метода је био да се направи прилог разумевању механизама који стоје иза интеракције биомолекула изолованих из гљиве Phomopsis  species са осам различитих типова рецептора који припадају патогеним бактеријама у обичајеном ултирезистентних на лекове. Резултати су указали на важне интеракције са рецепторима  3G7B  (Staphylococcus  aureus гиразаБ), 1F0K  (структура Escherichia  Coli трансферазе величине  1,9  Å)  и  1SHV  (SHV-1  β-лакта-маза Klebsiella pneumoniae) указујући на тај начин на рецепторе путем којих се започиње антибиотски одговор након активације најпотентнијим једињењима, 325-3, 325-5, фомо-енамидом и фомолом. Овим открићем се такође препоручује будући развој нових моћних антибиотика са широким спектром деловања базиран на структури изабраних молекула.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Molecular docking study on biomolecules isolated from endophytic fungi
T1  - Студија молекулског докинга са биомолекулима изолованим из ендофитних гљива
VL  - 86
IS  - 2
SP  - 125
EP  - 137
DO  - 10.2298/JSC200815002I
ER  - 

@article{
author = "Ignjatović, Janko and Đajić, Nevena and Krmar, Jovana and Protić, Ana and Štrukelj, Borut and Otašević, Biljana",
year = "2020",
abstract = "Recently, growing interest has been devoted to the investigation of compounds with antimicrobial activity due to rising cases of resistance of mic-robes to known therapies. A reliable and versatile source of novel drug disco-very was recently found among endophytic fungi. Hitherto, the research usu-ally  enclosed  the in  vitro  evaluation  of  antimicrobial  activity  and  chemical structure  elucidation  of  biomolecules  extracted  from  fungal  material.  There-fore, this research was designed as an extension to previous investigations of endophytic fungi growing on conifer needles by means of conducting a mole-cular docking study. The in  silico methods were used with the main goal to make a contribution to the understanding of the  mechanisms underlying the interaction of biomolecules isolated from fungus Phomopsis  species and eight different types of receptors that belong to usually multidrug resistant bacterial pathogens.  The  results  revealed  valuable  interactions  with  receptors  3G7B (Staphylococcus  aureus’s  gyrase  B),  1F0K  (1.9  Å  structure  of Escherichia coli’s  transferase)  and  1SHV  (Klebsiella  pneumoniae’s  SHV-1 β-lactamase) thus pointing out the receptors that trigger antibiotic response upon activation by  the  most  potent  compounds  325-3,  325-5,  phomoenamide  and  phomol. These findings also recommended further discovery of novel potent and broad-spectrum antibiotics based on the structure of selected molecules., У последње време, као одговорна повећање резистенције микроорганизама на познату терапију, све већа пажња се поклања истраживању једињења са антимикробном активношћу. Ендофитне гљиве су недавно представљене као поуздан и богат извор за развој нових лекова. До сада, истраживања су се углавном ограничавала на in vitro процену антимикробне активности и разоткривање хемијске структуре биомолекула изолованих из материјала гљива. Из тог разлога, ово истраживање је осмишљено као проширење претходно спроведених испитивања ендофита које расту на иглицама четинара путем in silico студије молекулског докинга. Главни циљ употребе in silico метода је био да се направи прилог разумевању механизама који стоје иза интеракције биомолекула изолованих из гљиве Phomopsis  species са осам различитих типова рецептора који припадају патогеним бактеријама у обичајеном ултирезистентних на лекове. Резултати су указали на важне интеракције са рецепторима  3G7B  (Staphylococcus  aureus гиразаБ), 1F0K  (структура Escherichia  Coli трансферазе величине  1,9  Å)  и  1SHV  (SHV-1  β-лакта-маза Klebsiella pneumoniae) указујући на тај начин на рецепторе путем којих се започиње антибиотски одговор након активације најпотентнијим једињењима, 325-3, 325-5, фомо-енамидом и фомолом. Овим открићем се такође препоручује будући развој нових моћних антибиотика са широким спектром деловања базиран на структури изабраних молекула.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Molecular docking study on biomolecules isolated from endophytic fungi, Студија молекулског докинга са биомолекулима изолованим из ендофитних гљива",
volume = "86",
number = "2",
pages = "125-137",
doi = "10.2298/JSC200815002I"
}

Ignjatović, J., Đajić, N., Krmar, J., Protić, A., Štrukelj, B.,& Otašević, B.. (2020). Molecular docking study on biomolecules isolated from endophytic fungi. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 86(2), 125-137.
https://doi.org/10.2298/JSC200815002I

Ignjatović J, Đajić N, Krmar J, Protić A, Štrukelj B, Otašević B. Molecular docking study on biomolecules isolated from endophytic fungi. in Journal of the Serbian Chemical Society. 2020;86(2):125-137.
doi:10.2298/JSC200815002I .

Ignjatović, Janko, Đajić, Nevena, Krmar, Jovana, Protić, Ana, Štrukelj, Borut, Otašević, Biljana, "Molecular docking study on biomolecules isolated from endophytic fungi" in Journal of the Serbian Chemical Society, 86, no. 2 (2020):125-137,
https://doi.org/10.2298/JSC200815002I . .

TY  - JOUR
AU  - Ignjatović, Janko
AU  - Maljurić, Nevena
AU  - Golubović, Jelena
AU  - Ravnikar, Matjaž
AU  - Petković, Miloš
AU  - Savodnik, Nika
AU  - Štrukelj, Borut
AU  - Otašević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3661
AB  - Recently, growing interest is devoted to investigation of bioactive secondary metabolites of endophytic fungi. Thus, as an extension to our previous achievements related to antimicrobial potential of endophytic fungi, Phomopsis species isolated from  conifer  needles  was  selected  as  appropriately  promising  natural  source  for  drug  discovery.  Its  dichloromethane  and  ethanol  extracts  considerably  inhibited  growth  of  Escherichia  coli  and  Staphylococcus  aureus.  Moreover,  the  indi-vidual compounds of dichloromethane extract have been separated, collected and purified using semi preparative liquid chromatographic analysis and comprehensively characterized using mass spectrometry (MS) and nuclear magnetic res-onance spectroscopy (NMR). Based on their antimicrobial activity and unique structural characteristics in comparison with  well-established  drugs  from  the  same  therapeutic  category,  two  dominant  compounds  (Z)-(Z)-2-acetoxyprop-1-en-1-yl-3-(3-((E)-3,4-dihydroxypent-1-en-1-yl)oxiran-2-yl)acrylate  (denoted  as  325-3)  and  (Z)-(Z)-2-acetoxyprop-1-en-1-yl  3-(3-((E)-4-hydroxy-3-oxopent-1-en-1-yl)oxiran-2-yl)acrylate  (denoted  as  325-5)  were  recognized  as  valuable  leading structures for future discovery of novel antibiotics.
PB  - Slovenian Chemical Society
T2  - Acta Chimica Slovenica
T1  - Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species
VL  - 67
IS  - 2
SP  - 445
EP  - 461
DO  - 10.17344/acsi.2019.5389
ER  - 

@article{
author = "Ignjatović, Janko and Maljurić, Nevena and Golubović, Jelena and Ravnikar, Matjaž and Petković, Miloš and Savodnik, Nika and Štrukelj, Borut and Otašević, Biljana",
year = "2020",
abstract = "Recently, growing interest is devoted to investigation of bioactive secondary metabolites of endophytic fungi. Thus, as an extension to our previous achievements related to antimicrobial potential of endophytic fungi, Phomopsis species isolated from  conifer  needles  was  selected  as  appropriately  promising  natural  source  for  drug  discovery.  Its  dichloromethane  and  ethanol  extracts  considerably  inhibited  growth  of  Escherichia  coli  and  Staphylococcus  aureus.  Moreover,  the  indi-vidual compounds of dichloromethane extract have been separated, collected and purified using semi preparative liquid chromatographic analysis and comprehensively characterized using mass spectrometry (MS) and nuclear magnetic res-onance spectroscopy (NMR). Based on their antimicrobial activity and unique structural characteristics in comparison with  well-established  drugs  from  the  same  therapeutic  category,  two  dominant  compounds  (Z)-(Z)-2-acetoxyprop-1-en-1-yl-3-(3-((E)-3,4-dihydroxypent-1-en-1-yl)oxiran-2-yl)acrylate  (denoted  as  325-3)  and  (Z)-(Z)-2-acetoxyprop-1-en-1-yl  3-(3-((E)-4-hydroxy-3-oxopent-1-en-1-yl)oxiran-2-yl)acrylate  (denoted  as  325-5)  were  recognized  as  valuable  leading structures for future discovery of novel antibiotics.",
publisher = "Slovenian Chemical Society",
journal = "Acta Chimica Slovenica",
title = "Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species",
volume = "67",
number = "2",
pages = "445-461",
doi = "10.17344/acsi.2019.5389"
}

Ignjatović, J., Maljurić, N., Golubović, J., Ravnikar, M., Petković, M., Savodnik, N., Štrukelj, B.,& Otašević, B.. (2020). Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species. in Acta Chimica Slovenica
Slovenian Chemical Society., 67(2), 445-461.
https://doi.org/10.17344/acsi.2019.5389

Ignjatović J, Maljurić N, Golubović J, Ravnikar M, Petković M, Savodnik N, Štrukelj B, Otašević B. Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species. in Acta Chimica Slovenica. 2020;67(2):445-461.
doi:10.17344/acsi.2019.5389 .

Ignjatović, Janko, Maljurić, Nevena, Golubović, Jelena, Ravnikar, Matjaž, Petković, Miloš, Savodnik, Nika, Štrukelj, Borut, Otašević, Biljana, "Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species" in Acta Chimica Slovenica, 67, no. 2 (2020):445-461,
https://doi.org/10.17344/acsi.2019.5389 . .

TY  - JOUR
AU  - Maljurić, Nevena
AU  - Golubović, Jelena
AU  - Ravnikar, Matjaz
AU  - Zigon, Dušan
AU  - Štrukelj, Borut
AU  - Otašević, Biljana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3182
AB  - Diabetes mellitus is one of the leading world's public health problems. Therefore, it is of a huge interest to develop new antidiabetic drugs. Apart from traditional therapy of diabetes, nowadays, importance is given to natural substances with antidiabetic potential. Fomes fomentarius is a mushroom widely used for different purposes, due to its range of already confirmed activities. Fomentariol is a constituent of Fomes fomentarius, responsible for its antidiabetic potential. In that respect, it is important to develop a method for isolation and quantification of fomentariol from fungal material, which will be simple and efficient. Multistep, complex extraction applied in the previously reported studies was avoided with ethanol, providing rapid single-step extraction. The presence of fomentariol in ethanolic extract was confirmed by high-resolution mass spectrometry. Semipreparative HPLC method was developed and applied for isolation from ethanol extract and purification of the active compound fomentariol. It was a gradient reversed-phase method with a mobile phase consisting of acetonitrile and 0.1% formic acid in water and total run time of 15 minutes. The amount of 6.5 mg of high-purity fomentariol was determined by quantitative NMR with toluene as internal standard. The isolated and determined amount of substance can be further used for the quantitative estimation of activity of fomentariol.
PB  - Hindawi Ltd, London
T2  - Journal of Analytical Methods in Chemistry
T1  - Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material
DO  - 10.1155/2018/2434691
ER  - 

@article{
author = "Maljurić, Nevena and Golubović, Jelena and Ravnikar, Matjaz and Zigon, Dušan and Štrukelj, Borut and Otašević, Biljana",
year = "2018",
abstract = "Diabetes mellitus is one of the leading world's public health problems. Therefore, it is of a huge interest to develop new antidiabetic drugs. Apart from traditional therapy of diabetes, nowadays, importance is given to natural substances with antidiabetic potential. Fomes fomentarius is a mushroom widely used for different purposes, due to its range of already confirmed activities. Fomentariol is a constituent of Fomes fomentarius, responsible for its antidiabetic potential. In that respect, it is important to develop a method for isolation and quantification of fomentariol from fungal material, which will be simple and efficient. Multistep, complex extraction applied in the previously reported studies was avoided with ethanol, providing rapid single-step extraction. The presence of fomentariol in ethanolic extract was confirmed by high-resolution mass spectrometry. Semipreparative HPLC method was developed and applied for isolation from ethanol extract and purification of the active compound fomentariol. It was a gradient reversed-phase method with a mobile phase consisting of acetonitrile and 0.1% formic acid in water and total run time of 15 minutes. The amount of 6.5 mg of high-purity fomentariol was determined by quantitative NMR with toluene as internal standard. The isolated and determined amount of substance can be further used for the quantitative estimation of activity of fomentariol.",
publisher = "Hindawi Ltd, London",
journal = "Journal of Analytical Methods in Chemistry",
title = "Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material",
doi = "10.1155/2018/2434691"
}

Maljurić, N., Golubović, J., Ravnikar, M., Zigon, D., Štrukelj, B.,& Otašević, B.. (2018). Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material. in Journal of Analytical Methods in Chemistry
Hindawi Ltd, London..
https://doi.org/10.1155/2018/2434691

Maljurić N, Golubović J, Ravnikar M, Zigon D, Štrukelj B, Otašević B. Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material. in Journal of Analytical Methods in Chemistry. 2018;.
doi:10.1155/2018/2434691 .

Maljurić, Nevena, Golubović, Jelena, Ravnikar, Matjaz, Zigon, Dušan, Štrukelj, Borut, Otašević, Biljana, "Isolation and Determination of Fomentariol: Novel Potential Antidiabetic Drug from Fungal Material" in Journal of Analytical Methods in Chemistry (2018),
https://doi.org/10.1155/2018/2434691 . .

TY  - JOUR
AU  - Injac, Rade
AU  - Mlinarić, Ales
AU  - Đorđević-Milić, Vukosava
AU  - Karljiković-Rajić, Katarina
AU  - Štrukelj, Borut
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1103
AB  - A separation technique for zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feedstuffs by micellar electrokinetic capillary chromatography (MEKC) was developed. The running buffer was 20 mmoll(-1) borate, 20 mmoll(-1) phosphate, pH 8.4, containing 20 mmoll(-1) sodium dodecylsulphate and 10% (v/v) methanol. MEKC was performed at 25C; the applied voltage was 25 kV with a running pressure of 10 mbar. Simultaneous UV detection for all analytes was at 215 nm. The method was validated for specificity, accuracy, linearity, precision and robustness. It was shown to be specific, accurate (recoveries were 99.7 +/- 0.3, 99.9 +/- 0.9, 99.8 +/- 1.0 and 99.5 +/- 0.4, respectively, for oxytetracycline-, sulfacetamide-, polymyxin B- and zinc bacitracin-spiked samples of feed for cow, pigs, chicken and cattle), linear over the tested range (correlation coefficients >= 0.9987) and precise (RSDs below 1.8% for each analyte). The method was applied to determine zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide as additives in animal feed.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment
T1  - Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography
VL  - 25
IS  - 4
SP  - 424
EP  - 431
DO  - 10.1080/02652030701584058
ER  - 

@article{
author = "Injac, Rade and Mlinarić, Ales and Đorđević-Milić, Vukosava and Karljiković-Rajić, Katarina and Štrukelj, Borut",
year = "2008",
abstract = "A separation technique for zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feedstuffs by micellar electrokinetic capillary chromatography (MEKC) was developed. The running buffer was 20 mmoll(-1) borate, 20 mmoll(-1) phosphate, pH 8.4, containing 20 mmoll(-1) sodium dodecylsulphate and 10% (v/v) methanol. MEKC was performed at 25C; the applied voltage was 25 kV with a running pressure of 10 mbar. Simultaneous UV detection for all analytes was at 215 nm. The method was validated for specificity, accuracy, linearity, precision and robustness. It was shown to be specific, accurate (recoveries were 99.7 +/- 0.3, 99.9 +/- 0.9, 99.8 +/- 1.0 and 99.5 +/- 0.4, respectively, for oxytetracycline-, sulfacetamide-, polymyxin B- and zinc bacitracin-spiked samples of feed for cow, pigs, chicken and cattle), linear over the tested range (correlation coefficients >= 0.9987) and precise (RSDs below 1.8% for each analyte). The method was applied to determine zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide as additives in animal feed.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment",
title = "Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography",
volume = "25",
number = "4",
pages = "424-431",
doi = "10.1080/02652030701584058"
}

Injac, R., Mlinarić, A., Đorđević-Milić, V., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography. in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment
Taylor & Francis Ltd, Abingdon., 25(4), 424-431.
https://doi.org/10.1080/02652030701584058

Injac R, Mlinarić A, Đorđević-Milić V, Karljiković-Rajić K, Štrukelj B. Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography. in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment. 2008;25(4):424-431.
doi:10.1080/02652030701584058 .

Injac, Rade, Mlinarić, Ales, Đorđević-Milić, Vukosava, Karljiković-Rajić, Katarina, Štrukelj, Borut, "Optimal conditions for determination of zinc bacitracin, polymyxin B, oxytetracycline and sulfacetamide in animal feed by micellar electrokinetic capillary chromatography" in Food Additives and Contaminants Part A: Chemistry Analysis Control Exposure & Risk Assessment, 25, no. 4 (2008):424-431,
https://doi.org/10.1080/02652030701584058 . .

TY  - JOUR
AU  - Injac, Rade
AU  - Srđenović, Branislava
AU  - Prijatelj, Matevz
AU  - Bošković, Marija
AU  - Karljiković-Rajić, Katarina
AU  - Štrukelj, Borut
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1053
PB  - Preston Publ Inc, Niles
T2  - Journal of Chromatographic Science
T1  - Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography
VL  - 46
IS  - 2
SP  - 137
EP  - 143
DO  - 10.1093/chromsci/46.2.137
ER  - 

@article{
author = "Injac, Rade and Srđenović, Branislava and Prijatelj, Matevz and Bošković, Marija and Karljiković-Rajić, Katarina and Štrukelj, Borut",
year = "2008",
publisher = "Preston Publ Inc, Niles",
journal = "Journal of Chromatographic Science",
title = "Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography",
volume = "46",
number = "2",
pages = "137-143",
doi = "10.1093/chromsci/46.2.137"
}

Injac, R., Srđenović, B., Prijatelj, M., Bošković, M., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography. in Journal of Chromatographic Science
Preston Publ Inc, Niles., 46(2), 137-143.
https://doi.org/10.1093/chromsci/46.2.137

Injac R, Srđenović B, Prijatelj M, Bošković M, Karljiković-Rajić K, Štrukelj B. Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography. in Journal of Chromatographic Science. 2008;46(2):137-143.
doi:10.1093/chromsci/46.2.137 .

Injac, Rade, Srđenović, Branislava, Prijatelj, Matevz, Bošković, Marija, Karljiković-Rajić, Katarina, Štrukelj, Borut, "Determination of caffeine and associated compounds in food, beverages, natural products, pharmaceuticals, and cosmetics by micellar electrokinetic capillary chromatography" in Journal of Chromatographic Science, 46, no. 2 (2008):137-143,
https://doi.org/10.1093/chromsci/46.2.137 . .

TY  - JOUR
AU  - Injac, Rade
AU  - Karljiković-Rajić, Katarina
AU  - Štrukelj, Borut
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1134
AB  - Micellar electrokinetic capillary chromatography (MEKC) has become a popular mode among the several capillary electro-migration techniques. Most drug analysis can be performed by using MEKC because of its wide applicability. Separation of very complex mixtures, determination of drugs in the biological materials, etc., can be successfully achieved by MEKC. This review surveys typical applications of MEKC analysis. Recent advances in MEKC, especially with solid-phase extraction and large-volume sample stacking, are described. Modes of electrokinetic chromatography including MEKC, a separation theory of MEKC, environmental friendly analysis, and selectivity manipulation in MEKC are also briefly mentioned.
AB  - Micelama elektrokinetička kapilarna hromatografija (MEKC) postala je najpopularnija kapilarna elektromigraciona metoda. Na osnovu veoma široke upotrebe metode, većina analiza lekova moguća je uz MEKC. Separacija veoma kompleksnih smeša, određivanje lekova u biološkom materijalu, i analize drugih uzoraka, mogu biti veoma uspešno izvedene upotrebom MEKC tehnike. U okviru ovog preglednog rada prikazana je aplikacija tipičnih MEKC metoda. Noviji pristupi u kombinaciji sa čvrsto-faznom ekstrakcijom i visoko-volumskim injektovanjem su takođe opisani. Teorijski i ekološki pristup, kao i prednosti i nedostatci same metode, prikazani su teorijski i kroz praktične primere.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Application of micellar electrokinetic capillary chromatography for routine analysis of different materials
T1  - Upotreba micelarne elektrokinetičke kapilarne hromatografije u rutinskoj analizi različitih uzoraka
VL  - 62
IS  - 3
SP  - 181
EP  - 190
DO  - 10.2298/HEMIND0803181I
ER  - 

@article{
author = "Injac, Rade and Karljiković-Rajić, Katarina and Štrukelj, Borut",
year = "2008",
abstract = "Micellar electrokinetic capillary chromatography (MEKC) has become a popular mode among the several capillary electro-migration techniques. Most drug analysis can be performed by using MEKC because of its wide applicability. Separation of very complex mixtures, determination of drugs in the biological materials, etc., can be successfully achieved by MEKC. This review surveys typical applications of MEKC analysis. Recent advances in MEKC, especially with solid-phase extraction and large-volume sample stacking, are described. Modes of electrokinetic chromatography including MEKC, a separation theory of MEKC, environmental friendly analysis, and selectivity manipulation in MEKC are also briefly mentioned., Micelama elektrokinetička kapilarna hromatografija (MEKC) postala je najpopularnija kapilarna elektromigraciona metoda. Na osnovu veoma široke upotrebe metode, većina analiza lekova moguća je uz MEKC. Separacija veoma kompleksnih smeša, određivanje lekova u biološkom materijalu, i analize drugih uzoraka, mogu biti veoma uspešno izvedene upotrebom MEKC tehnike. U okviru ovog preglednog rada prikazana je aplikacija tipičnih MEKC metoda. Noviji pristupi u kombinaciji sa čvrsto-faznom ekstrakcijom i visoko-volumskim injektovanjem su takođe opisani. Teorijski i ekološki pristup, kao i prednosti i nedostatci same metode, prikazani su teorijski i kroz praktične primere.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Application of micellar electrokinetic capillary chromatography for routine analysis of different materials, Upotreba micelarne elektrokinetičke kapilarne hromatografije u rutinskoj analizi različitih uzoraka",
volume = "62",
number = "3",
pages = "181-190",
doi = "10.2298/HEMIND0803181I"
}

Injac, R., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). Application of micellar electrokinetic capillary chromatography for routine analysis of different materials. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 62(3), 181-190.
https://doi.org/10.2298/HEMIND0803181I

Injac R, Karljiković-Rajić K, Štrukelj B. Application of micellar electrokinetic capillary chromatography for routine analysis of different materials. in Hemijska industrija. 2008;62(3):181-190.
doi:10.2298/HEMIND0803181I .

Injac, Rade, Karljiković-Rajić, Katarina, Štrukelj, Borut, "Application of micellar electrokinetic capillary chromatography for routine analysis of different materials" in Hemijska industrija, 62, no. 3 (2008):181-190,
https://doi.org/10.2298/HEMIND0803181I . .

TY  - JOUR
AU  - Injac, Rade
AU  - Kac, Javor
AU  - Karljiković-Rajić, Katarina
AU  - Štrukelj, Borut
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1110
AB  - A micellar electrokinetic capillary chromatography was performed at 25 degrees C and 30 kV (under pressure of 15 mbar), with 30 mM berate buffer (pH 9.0), 60 mM sodium dodecysulfate, and 10% (v/v) ethanol as background electrolyte for the determination of sulfamethoxazole and trimethoprim. UV detection was at 205 nm. Recoveries were optimal and acceptable after extraction with ethanol / deionized water (1:1, v/v) for both investigated compounds from laboratory mixtures of standards. The method was shown to be specific, accurate (recoveries were 99.9 +/- 0.4% for sulfamethoxazole and 99.8 +/- 0.3% for trimethoprim), linear over the tested ranges (correlation coefficients >= 0.9990) and precise (RSD below 0.6%). The method was applied to determine sulfamethoxazole and trimethoprim in tablets, powder for cutaneous use and solution for infusion.
PB  - Food & Drug Adminstration, Taipei
T2  - Journal of Food and Drug Analysis
T1  - Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography
VL  - 16
IS  - 1
SP  - 18
EP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1110
ER  - 

@article{
author = "Injac, Rade and Kac, Javor and Karljiković-Rajić, Katarina and Štrukelj, Borut",
year = "2008",
abstract = "A micellar electrokinetic capillary chromatography was performed at 25 degrees C and 30 kV (under pressure of 15 mbar), with 30 mM berate buffer (pH 9.0), 60 mM sodium dodecysulfate, and 10% (v/v) ethanol as background electrolyte for the determination of sulfamethoxazole and trimethoprim. UV detection was at 205 nm. Recoveries were optimal and acceptable after extraction with ethanol / deionized water (1:1, v/v) for both investigated compounds from laboratory mixtures of standards. The method was shown to be specific, accurate (recoveries were 99.9 +/- 0.4% for sulfamethoxazole and 99.8 +/- 0.3% for trimethoprim), linear over the tested ranges (correlation coefficients >= 0.9990) and precise (RSD below 0.6%). The method was applied to determine sulfamethoxazole and trimethoprim in tablets, powder for cutaneous use and solution for infusion.",
publisher = "Food & Drug Adminstration, Taipei",
journal = "Journal of Food and Drug Analysis",
title = "Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography",
volume = "16",
number = "1",
pages = "18-25",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1110"
}

Injac, R., Kac, J., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography. in Journal of Food and Drug Analysis
Food & Drug Adminstration, Taipei., 16(1), 18-25.
https://hdl.handle.net/21.15107/rcub_farfar_1110

Injac R, Kac J, Karljiković-Rajić K, Štrukelj B. Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography. in Journal of Food and Drug Analysis. 2008;16(1):18-25.
https://hdl.handle.net/21.15107/rcub_farfar_1110 .

Injac, Rade, Kac, Javor, Karljiković-Rajić, Katarina, Štrukelj, Borut, "Optimal conditions for extraction and simultaneous determination of sulfamethoxazole and trimethoprim in pharmaceuticals by micellar electrokinetic capillary chromatography" in Journal of Food and Drug Analysis, 16, no. 1 (2008):18-25,
https://hdl.handle.net/21.15107/rcub_farfar_1110 .

TY  - JOUR
AU  - Injac, Rade
AU  - Karljiković-Rajić, Katarina
AU  - Štrukelj, Borut
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1061
AB  - A novel and simple method has been developed for the determination of doxycycline (DOX) in biological fluids. The method is based on SPE, large-volume sample stacking (LVSS) and MEKC with UV-DAD detection. Six SPE cartridges have been used in investigation for sample clean up and pre-concentration (Supelco (R) LC-8, LC-18, LC-SCX, and LC-WCX, as well as Strata (TM)-X and X-C). DOX was determined on a 56 cm. (effective length 50 cm) x 50 mu m id fused-silica capillary. The BGE was 20 mM borate buffer, pH 9.3, containing 80 mM SDS and 7.5% v/v of methanol (30 s x 50 mbar), and the temperature and voltage were 25 degrees C and 30 kV, respectively. The analytical wavelength was set at 210 nm. Under optimized conditions it is possible to determine DOX in human serum, urine, semen, tears and saliva with recovery of 97.5% (RSD 2.5%). The method was shown to be sensitive (LOD is 1 mu g/L) and precise (intra-day RSD 0.2 and 2.4%; inter-days 0.4 and 3.5% for migration time and peak area, respectively). Results for developed SPE-LVSS-MEKC were compared with LVSS-MEKC method with direct sample injection. The new LVSS-MEKC method is presented as a useful technique for rapid determination without extraction procedure of DOX in human urine and serum, using 80mM of SDS, 10% v/v of methanol and 40 mM borate buffer (pH 9.3; 30 s x 50 mbar; 25 degrees C; 30 kV; 350 nm), but not for the other biological fluids, according to lower sensitivity of the method and because of the sample composition.
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Electrophoresis
T1  - SPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: Comparison of direct injection to SPE-MEKC
VL  - 29
IS  - 21
SP  - 4431
EP  - 4438
DO  - 10.1002/elps.200800339
ER  - 

@article{
author = "Injac, Rade and Karljiković-Rajić, Katarina and Štrukelj, Borut",
year = "2008",
abstract = "A novel and simple method has been developed for the determination of doxycycline (DOX) in biological fluids. The method is based on SPE, large-volume sample stacking (LVSS) and MEKC with UV-DAD detection. Six SPE cartridges have been used in investigation for sample clean up and pre-concentration (Supelco (R) LC-8, LC-18, LC-SCX, and LC-WCX, as well as Strata (TM)-X and X-C). DOX was determined on a 56 cm. (effective length 50 cm) x 50 mu m id fused-silica capillary. The BGE was 20 mM borate buffer, pH 9.3, containing 80 mM SDS and 7.5% v/v of methanol (30 s x 50 mbar), and the temperature and voltage were 25 degrees C and 30 kV, respectively. The analytical wavelength was set at 210 nm. Under optimized conditions it is possible to determine DOX in human serum, urine, semen, tears and saliva with recovery of 97.5% (RSD 2.5%). The method was shown to be sensitive (LOD is 1 mu g/L) and precise (intra-day RSD 0.2 and 2.4%; inter-days 0.4 and 3.5% for migration time and peak area, respectively). Results for developed SPE-LVSS-MEKC were compared with LVSS-MEKC method with direct sample injection. The new LVSS-MEKC method is presented as a useful technique for rapid determination without extraction procedure of DOX in human urine and serum, using 80mM of SDS, 10% v/v of methanol and 40 mM borate buffer (pH 9.3; 30 s x 50 mbar; 25 degrees C; 30 kV; 350 nm), but not for the other biological fluids, according to lower sensitivity of the method and because of the sample composition.",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Electrophoresis",
title = "SPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: Comparison of direct injection to SPE-MEKC",
volume = "29",
number = "21",
pages = "4431-4438",
doi = "10.1002/elps.200800339"
}

Injac, R., Karljiković-Rajić, K.,& Štrukelj, B.. (2008). SPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: Comparison of direct injection to SPE-MEKC. in Electrophoresis
Wiley-VCH Verlag GMBH, Weinheim., 29(21), 4431-4438.
https://doi.org/10.1002/elps.200800339

Injac R, Karljiković-Rajić K, Štrukelj B. SPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: Comparison of direct injection to SPE-MEKC. in Electrophoresis. 2008;29(21):4431-4438.
doi:10.1002/elps.200800339 .

Injac, Rade, Karljiković-Rajić, Katarina, Štrukelj, Borut, "SPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: Comparison of direct injection to SPE-MEKC" in Electrophoresis, 29, no. 21 (2008):4431-4438,
https://doi.org/10.1002/elps.200800339 . .