TY  - THES
AU  - Ignjatović, Janko
PY  - 2022
UR  - https://eteze.bg.ac.rs/application/showtheses?thesesId=9083
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:29313/bdef:Content/download
UR  - https://plus.cobiss.net/cobiss/sr/sr/bib/77500937
UR  - https://nardus.mpn.gov.rs/handle/123456789/21428
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4966
AB  - Rezistencija bakterija na delovanje antibiotika predstavlja globalni problem. Na polju razvojanovih lekova iz prirodnih izvora, nedavno je prepoznat potencijal endofitnih gljiva zahvaljujućisposobnosti da proizvedu sekundarne metabolite različitih bioloških aktivnosti. Biosinteza ovihjedinjenja je pod velikim uticajem brojnih faktora koji se vezuju za izbor biljke domaćina,klimatske uslove, ishranu i prisustvo drugih mikroorganizama u okruženju. Velika bazapodataka vezanih za aktivnost endofitnih gljiva prema patogenim bakterijama pretaživana jeprimenom metode analize glavnih komponenti sa ciljem pronalaženja obrazaca u podacima kojibi ukazali na manji broj pravih kandidata za novih razvoj lekova. Na ovaj način, osvetljen jeantimikrobni karakter gljive Phomopsis species. In vitro testovima je potvrđeno dadihlormetanski ekstrakt gljive izolovane iz četina bora inhibira rast bakterija Escherichia coli iStaphylococcus aureus. Hromatografsko razdvajanje pojedinačnih jedinjenja ekstraktaoptimizovano je primenom dizajna eksperimenata, a zatim je izvršeno izolovanje ikarakterizacija njihove hemijske strukture korišćenjem masene spektrometrije i NMRspektroskopije. In silico metodama su definisani prediktori bioraspoloživosti i toksikološkeaktivnosti jedinjenja (Z)-(Z)-2-acetoksiprop-1-en-1-il-3-(3-((E)-3,4-dihidroksipent-1-en-1-il)oksiran-2-il)akrilat i (Z)-(Z)-2-acetoksiprop-1-en-1-il-(3-((E)-4-hidroksi-3-oksopent-1-en-1-il)oksiran-2-il)akrilat. Za razliku od dosadašnih istraživanja koja su se ograničavala in vitrotestovima antimikrobne aktivnosti endofitnih gljiva i razrešavanjem hemijske struktureizolovanih biomolekula, ova disertacija predstavlja proširenje prethodnih istraživanjaprimenom in silico metoda. Studija molekulskog dokinga omogućila je razumevanjemehanizama interakcije biomolekula sa receptorima koji pripadaju patogenim bakterijamauobičajeno multirezistentnim na antibiotike. Primenom veštačkih neuronskih mreža nagrađenisu pouzdani modeli koji ukazuju na vezu između hemijske strukture, parametara interakcije iafiniteta vezivanja za receptore na osnovu kojih je moguć razvoj novih hemijski srodnih antibiotika.
AB  - Bacterial resistance towards antibiotics represents a global phenomenon. Potential ofendophytic fungi as producers of secondary metabolites with wide spectra of differentbioactivities in the field of drug discovery from natural resources has recently been introduced.The production of these compounds is under great impact of variety of factors related to thechoice of plant host, climate conditions, nutrition and presence of other microorganisms in thesame surrounding. Big data set comprising of indices of endophytic fungi antibacterial activitytowards patogen bacteria was evaluated using principal component analysis with the aim to findpatterns in data and to point out to a limited number of proper candidates for futurepharmaceutical research. This resulted in highlightening of the antimicrobial character ofPhomopsis species. In vitro tests proved that dichloromethane extract of endophytic fungiisolated from conifer needles inhibits the growth of Escherichia coli and Staphylococcusaureus. Chromatographic separation of individual components of extract was optimized usingdesign of experiments followed by the isolation and chemical structure characterization usingmass spectrometry and NMR spectroscopy. In silico methods were used to define the predictorsof bioavaliability and toxicological activity of compounds (Z)-(Z)-2-acetoxyprop-1-en-1-yl-3-(3-((E)-3,4-dihydroxypent-1-en-1-yl)oxiran-2-yl)acrylate and (Z)-(Z)-2-acetoxyprop-1-en-1-yl-3-(3-((E)-4-hydroxy-3-oxopent-1-en-1-yl)oxiran-2-yl)acrylate. Unlike up to date researchoutcomes limited to in vitro evaluation of antimicrobial activity of endophytic fungi andchemical structure elucidation of isolated biomolecules, this disertation represents an extensionto previous investigations using in silico methods. The molecular docking study enabled thecomprehensive understanding of the mechanisms underlying the interaction of biomoleculeswith receptors belonging to usually multidrug resistant bacterial pathogens. The artificial neuralnetworks were used to build reliable models relating chemical structure, parameters ofinteraction and the binding affinity to receptors, thus providing the essence for futuredevelopment of new chemically related antibiotics.
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Istraživanje antimikrobne aktivnosti i hromatografskog ponašanja sastojaka endofitnih gljiva primenom hemometrijskih metoda
UR  - https://hdl.handle.net/21.15107/rcub_nardus_21428
ER  - 

@phdthesis{
author = "Ignjatović, Janko",
year = "2022",
abstract = "Rezistencija bakterija na delovanje antibiotika predstavlja globalni problem. Na polju razvojanovih lekova iz prirodnih izvora, nedavno je prepoznat potencijal endofitnih gljiva zahvaljujućisposobnosti da proizvedu sekundarne metabolite različitih bioloških aktivnosti. Biosinteza ovihjedinjenja je pod velikim uticajem brojnih faktora koji se vezuju za izbor biljke domaćina,klimatske uslove, ishranu i prisustvo drugih mikroorganizama u okruženju. Velika bazapodataka vezanih za aktivnost endofitnih gljiva prema patogenim bakterijama pretaživana jeprimenom metode analize glavnih komponenti sa ciljem pronalaženja obrazaca u podacima kojibi ukazali na manji broj pravih kandidata za novih razvoj lekova. Na ovaj način, osvetljen jeantimikrobni karakter gljive Phomopsis species. In vitro testovima je potvrđeno dadihlormetanski ekstrakt gljive izolovane iz četina bora inhibira rast bakterija Escherichia coli iStaphylococcus aureus. Hromatografsko razdvajanje pojedinačnih jedinjenja ekstraktaoptimizovano je primenom dizajna eksperimenata, a zatim je izvršeno izolovanje ikarakterizacija njihove hemijske strukture korišćenjem masene spektrometrije i NMRspektroskopije. In silico metodama su definisani prediktori bioraspoloživosti i toksikološkeaktivnosti jedinjenja (Z)-(Z)-2-acetoksiprop-1-en-1-il-3-(3-((E)-3,4-dihidroksipent-1-en-1-il)oksiran-2-il)akrilat i (Z)-(Z)-2-acetoksiprop-1-en-1-il-(3-((E)-4-hidroksi-3-oksopent-1-en-1-il)oksiran-2-il)akrilat. Za razliku od dosadašnih istraživanja koja su se ograničavala in vitrotestovima antimikrobne aktivnosti endofitnih gljiva i razrešavanjem hemijske struktureizolovanih biomolekula, ova disertacija predstavlja proširenje prethodnih istraživanjaprimenom in silico metoda. Studija molekulskog dokinga omogućila je razumevanjemehanizama interakcije biomolekula sa receptorima koji pripadaju patogenim bakterijamauobičajeno multirezistentnim na antibiotike. Primenom veštačkih neuronskih mreža nagrađenisu pouzdani modeli koji ukazuju na vezu između hemijske strukture, parametara interakcije iafiniteta vezivanja za receptore na osnovu kojih je moguć razvoj novih hemijski srodnih antibiotika., Bacterial resistance towards antibiotics represents a global phenomenon. Potential ofendophytic fungi as producers of secondary metabolites with wide spectra of differentbioactivities in the field of drug discovery from natural resources has recently been introduced.The production of these compounds is under great impact of variety of factors related to thechoice of plant host, climate conditions, nutrition and presence of other microorganisms in thesame surrounding. Big data set comprising of indices of endophytic fungi antibacterial activitytowards patogen bacteria was evaluated using principal component analysis with the aim to findpatterns in data and to point out to a limited number of proper candidates for futurepharmaceutical research. This resulted in highlightening of the antimicrobial character ofPhomopsis species. In vitro tests proved that dichloromethane extract of endophytic fungiisolated from conifer needles inhibits the growth of Escherichia coli and Staphylococcusaureus. Chromatographic separation of individual components of extract was optimized usingdesign of experiments followed by the isolation and chemical structure characterization usingmass spectrometry and NMR spectroscopy. In silico methods were used to define the predictorsof bioavaliability and toxicological activity of compounds (Z)-(Z)-2-acetoxyprop-1-en-1-yl-3-(3-((E)-3,4-dihydroxypent-1-en-1-yl)oxiran-2-yl)acrylate and (Z)-(Z)-2-acetoxyprop-1-en-1-yl-3-(3-((E)-4-hydroxy-3-oxopent-1-en-1-yl)oxiran-2-yl)acrylate. Unlike up to date researchoutcomes limited to in vitro evaluation of antimicrobial activity of endophytic fungi andchemical structure elucidation of isolated biomolecules, this disertation represents an extensionto previous investigations using in silico methods. The molecular docking study enabled thecomprehensive understanding of the mechanisms underlying the interaction of biomoleculeswith receptors belonging to usually multidrug resistant bacterial pathogens. The artificial neuralnetworks were used to build reliable models relating chemical structure, parameters ofinteraction and the binding affinity to receptors, thus providing the essence for futuredevelopment of new chemically related antibiotics.",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Istraživanje antimikrobne aktivnosti i hromatografskog ponašanja sastojaka endofitnih gljiva primenom hemometrijskih metoda",
url = "https://hdl.handle.net/21.15107/rcub_nardus_21428"
}

Ignjatović, J.. (2022). Istraživanje antimikrobne aktivnosti i hromatografskog ponašanja sastojaka endofitnih gljiva primenom hemometrijskih metoda. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_21428

Ignjatović J. Istraživanje antimikrobne aktivnosti i hromatografskog ponašanja sastojaka endofitnih gljiva primenom hemometrijskih metoda. in Универзитет у Београду. 2022;.
https://hdl.handle.net/21.15107/rcub_nardus_21428 .

Ignjatović, Janko, "Istraživanje antimikrobne aktivnosti i hromatografskog ponašanja sastojaka endofitnih gljiva primenom hemometrijskih metoda" in Универзитет у Београду (2022),
https://hdl.handle.net/21.15107/rcub_nardus_21428 .

TY  - JOUR
AU  - Ignjatović, Janko
AU  - Đajić, Nevena
AU  - Krmar, Jovana
AU  - Protić, Ana
AU  - Štrukelj, Borut
AU  - Otašević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3797
AB  - Recently, growing interest has been devoted to the investigation of compounds with antimicrobial activity due to rising cases of resistance of mic-robes to known therapies. A reliable and versatile source of novel drug disco-very was recently found among endophytic fungi. Hitherto, the research usu-ally  enclosed  the in  vitro  evaluation  of  antimicrobial  activity  and  chemical structure  elucidation  of  biomolecules  extracted  from  fungal  material.  There-fore, this research was designed as an extension to previous investigations of endophytic fungi growing on conifer needles by means of conducting a mole-cular docking study. The in  silico methods were used with the main goal to make a contribution to the understanding of the  mechanisms underlying the interaction of biomolecules isolated from fungus Phomopsis  species and eight different types of receptors that belong to usually multidrug resistant bacterial pathogens.  The  results  revealed  valuable  interactions  with  receptors  3G7B (Staphylococcus  aureus’s  gyrase  B),  1F0K  (1.9  Å  structure  of Escherichia coli’s  transferase)  and  1SHV  (Klebsiella  pneumoniae’s  SHV-1 β-lactamase) thus pointing out the receptors that trigger antibiotic response upon activation by  the  most  potent  compounds  325-3,  325-5,  phomoenamide  and  phomol. These findings also recommended further discovery of novel potent and broad-spectrum antibiotics based on the structure of selected molecules.
AB  - У последње време, као одговорна повећање резистенције микроорганизама на познату терапију, све већа пажња се поклања истраживању једињења са антимикробном активношћу. Ендофитне гљиве су недавно представљене као поуздан и богат извор за развој нових лекова. До сада, истраживања су се углавном ограничавала на in vitro процену антимикробне активности и разоткривање хемијске структуре биомолекула изолованих из материјала гљива. Из тог разлога, ово истраживање је осмишљено као проширење претходно спроведених испитивања ендофита које расту на иглицама четинара путем in silico студије молекулског докинга. Главни циљ употребе in silico метода је био да се направи прилог разумевању механизама који стоје иза интеракције биомолекула изолованих из гљиве Phomopsis  species са осам различитих типова рецептора који припадају патогеним бактеријама у обичајеном ултирезистентних на лекове. Резултати су указали на важне интеракције са рецепторима  3G7B  (Staphylococcus  aureus гиразаБ), 1F0K  (структура Escherichia  Coli трансферазе величине  1,9  Å)  и  1SHV  (SHV-1  β-лакта-маза Klebsiella pneumoniae) указујући на тај начин на рецепторе путем којих се започиње антибиотски одговор након активације најпотентнијим једињењима, 325-3, 325-5, фомо-енамидом и фомолом. Овим открићем се такође препоручује будући развој нових моћних антибиотика са широким спектром деловања базиран на структури изабраних молекула.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Molecular docking study on biomolecules isolated from endophytic fungi
T1  - Студија молекулског докинга са биомолекулима изолованим из ендофитних гљива
VL  - 86
IS  - 2
SP  - 125
EP  - 137
DO  - 10.2298/JSC200815002I
ER  - 

@article{
author = "Ignjatović, Janko and Đajić, Nevena and Krmar, Jovana and Protić, Ana and Štrukelj, Borut and Otašević, Biljana",
year = "2020",
abstract = "Recently, growing interest has been devoted to the investigation of compounds with antimicrobial activity due to rising cases of resistance of mic-robes to known therapies. A reliable and versatile source of novel drug disco-very was recently found among endophytic fungi. Hitherto, the research usu-ally  enclosed  the in  vitro  evaluation  of  antimicrobial  activity  and  chemical structure  elucidation  of  biomolecules  extracted  from  fungal  material.  There-fore, this research was designed as an extension to previous investigations of endophytic fungi growing on conifer needles by means of conducting a mole-cular docking study. The in  silico methods were used with the main goal to make a contribution to the understanding of the  mechanisms underlying the interaction of biomolecules isolated from fungus Phomopsis  species and eight different types of receptors that belong to usually multidrug resistant bacterial pathogens.  The  results  revealed  valuable  interactions  with  receptors  3G7B (Staphylococcus  aureus’s  gyrase  B),  1F0K  (1.9  Å  structure  of Escherichia coli’s  transferase)  and  1SHV  (Klebsiella  pneumoniae’s  SHV-1 β-lactamase) thus pointing out the receptors that trigger antibiotic response upon activation by  the  most  potent  compounds  325-3,  325-5,  phomoenamide  and  phomol. These findings also recommended further discovery of novel potent and broad-spectrum antibiotics based on the structure of selected molecules., У последње време, као одговорна повећање резистенције микроорганизама на познату терапију, све већа пажња се поклања истраживању једињења са антимикробном активношћу. Ендофитне гљиве су недавно представљене као поуздан и богат извор за развој нових лекова. До сада, истраживања су се углавном ограничавала на in vitro процену антимикробне активности и разоткривање хемијске структуре биомолекула изолованих из материјала гљива. Из тог разлога, ово истраживање је осмишљено као проширење претходно спроведених испитивања ендофита које расту на иглицама четинара путем in silico студије молекулског докинга. Главни циљ употребе in silico метода је био да се направи прилог разумевању механизама који стоје иза интеракције биомолекула изолованих из гљиве Phomopsis  species са осам различитих типова рецептора који припадају патогеним бактеријама у обичајеном ултирезистентних на лекове. Резултати су указали на важне интеракције са рецепторима  3G7B  (Staphylococcus  aureus гиразаБ), 1F0K  (структура Escherichia  Coli трансферазе величине  1,9  Å)  и  1SHV  (SHV-1  β-лакта-маза Klebsiella pneumoniae) указујући на тај начин на рецепторе путем којих се започиње антибиотски одговор након активације најпотентнијим једињењима, 325-3, 325-5, фомо-енамидом и фомолом. Овим открићем се такође препоручује будући развој нових моћних антибиотика са широким спектром деловања базиран на структури изабраних молекула.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Molecular docking study on biomolecules isolated from endophytic fungi, Студија молекулског докинга са биомолекулима изолованим из ендофитних гљива",
volume = "86",
number = "2",
pages = "125-137",
doi = "10.2298/JSC200815002I"
}

Ignjatović, J., Đajić, N., Krmar, J., Protić, A., Štrukelj, B.,& Otašević, B.. (2020). Molecular docking study on biomolecules isolated from endophytic fungi. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 86(2), 125-137.
https://doi.org/10.2298/JSC200815002I

Ignjatović J, Đajić N, Krmar J, Protić A, Štrukelj B, Otašević B. Molecular docking study on biomolecules isolated from endophytic fungi. in Journal of the Serbian Chemical Society. 2020;86(2):125-137.
doi:10.2298/JSC200815002I .

Ignjatović, Janko, Đajić, Nevena, Krmar, Jovana, Protić, Ana, Štrukelj, Borut, Otašević, Biljana, "Molecular docking study on biomolecules isolated from endophytic fungi" in Journal of the Serbian Chemical Society, 86, no. 2 (2020):125-137,
https://doi.org/10.2298/JSC200815002I . .

TY  - JOUR
AU  - Otašević, Biljana
AU  - Krmar, Jovana
AU  - Đajić, Nevena
AU  - Ignjatović, Janko
AU  - Protić, Ana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3662
AB  - Drug resistance, especially bacterial antibiotic resistance, is recognized as a global phenomenon. Potential of endophytic fungi as producers of secondary metabolites with wide spectra of different bioactivities in the field of drug discovery has recently been introduced. The production of these compounds is under the great impact of the variety of factors related to the choice of plant host, climate conditions, nutrition, presence of other microorganisms in the same surrounding, etc. Due to a large number of different indices of endophytic fungi antibacterial activity, critical evaluation with the assistance of data mining analytical methods was performed. The activity towards several pathogen bacteria of endophytes species growing on different plant hosts, deciduous trees as well as herbaceous plants spread worldwide in different climatic zones together with the taxonomy of endophytes was taken into consideration. The principal component analysis was used to explore formed big data set and through finding patterns in data, to point out to a limited number of proper candidates for future pharmaceutical research. The antimicrobial character of Phomopsis species was highlighted and future perspectives in their therapeutic use were projected.
AB  - Rezistencija na lekove, posebno rezistencija bakterija na delovanje antibiotika, smatra se globalnim  problemom.  Na  polju  razvoja  novih  lekova  nedavno  su  se  pojavile  endofitne  gljive zahvaljujući  sposobnosti  da  proizvedu  sekundarne  metabolite  sa  širokim  spektrom  različitih bioloških aktivnosti. Biosinteza ovih jedinjenja je pod velikim uticajem brojnih faktora koji se vezuju za izbor biljke domaćina, klimatske uslove, ishranu, prisustvo drugih mikroorganizama u istom okruženju itd. Pošto je u literaturi primećen veliki broj različitih indikatora antibakterijske aktivnosti endofitnih gljiva, izvršena je njihova kritička procena korišćenjem analitičkih metoda pretrage  podataka.  Razmatrana je  aktivnost  prema  nekoliko  sojeva  patogenih  bakterija  koje  su pokazale  endofitne gljive koje rastu na različitim biljkama domaćinima, listopadnom drveću i zeljastim  biljkama  širom  sveta  i  u  različitim  klimatskim  uslovima,  kao  i  taksonomska rasprostranjenost  vrsta  endofitnih  gljiva. Istraživanje velike grupe podataka je vršeno pomoću analize  glavnih  komponenata  (eng. Principal  component  analysis)  sa  ciljem  pronalaženja obrazaca  u  podacima  koji  bi  ukazali  na  manji  broj  pravih  kandidata  za  dalji  razvoj  lekova. Osvetljen  je  antimikrobni  karakter Phomopsis vrsta, a takođe su procenjeni i budući pravci u njegovoj terapijskoj primeni.
PB  - Pharmaceutical Association of Serbia
T2  - Arhiv za farmaciju
T1  - Chemometric window to antimicrobial activity of biomolecules isolated from endophytic fungi
T1  - Hemometrijski prozor ka antibakterijskoj aktivnosti biomolekula izolovanih iz endofitnih gljiva
VL  - 70
IS  - 3
SP  - 142
EP  - 156
DO  - 10.5937/arhfarm2003142O
ER  - 

@article{
author = "Otašević, Biljana and Krmar, Jovana and Đajić, Nevena and Ignjatović, Janko and Protić, Ana",
year = "2020",
abstract = "Drug resistance, especially bacterial antibiotic resistance, is recognized as a global phenomenon. Potential of endophytic fungi as producers of secondary metabolites with wide spectra of different bioactivities in the field of drug discovery has recently been introduced. The production of these compounds is under the great impact of the variety of factors related to the choice of plant host, climate conditions, nutrition, presence of other microorganisms in the same surrounding, etc. Due to a large number of different indices of endophytic fungi antibacterial activity, critical evaluation with the assistance of data mining analytical methods was performed. The activity towards several pathogen bacteria of endophytes species growing on different plant hosts, deciduous trees as well as herbaceous plants spread worldwide in different climatic zones together with the taxonomy of endophytes was taken into consideration. The principal component analysis was used to explore formed big data set and through finding patterns in data, to point out to a limited number of proper candidates for future pharmaceutical research. The antimicrobial character of Phomopsis species was highlighted and future perspectives in their therapeutic use were projected., Rezistencija na lekove, posebno rezistencija bakterija na delovanje antibiotika, smatra se globalnim  problemom.  Na  polju  razvoja  novih  lekova  nedavno  su  se  pojavile  endofitne  gljive zahvaljujući  sposobnosti  da  proizvedu  sekundarne  metabolite  sa  širokim  spektrom  različitih bioloških aktivnosti. Biosinteza ovih jedinjenja je pod velikim uticajem brojnih faktora koji se vezuju za izbor biljke domaćina, klimatske uslove, ishranu, prisustvo drugih mikroorganizama u istom okruženju itd. Pošto je u literaturi primećen veliki broj različitih indikatora antibakterijske aktivnosti endofitnih gljiva, izvršena je njihova kritička procena korišćenjem analitičkih metoda pretrage  podataka.  Razmatrana je  aktivnost  prema  nekoliko  sojeva  patogenih  bakterija  koje  su pokazale  endofitne gljive koje rastu na različitim biljkama domaćinima, listopadnom drveću i zeljastim  biljkama  širom  sveta  i  u  različitim  klimatskim  uslovima,  kao  i  taksonomska rasprostranjenost  vrsta  endofitnih  gljiva. Istraživanje velike grupe podataka je vršeno pomoću analize  glavnih  komponenata  (eng. Principal  component  analysis)  sa  ciljem  pronalaženja obrazaca  u  podacima  koji  bi  ukazali  na  manji  broj  pravih  kandidata  za  dalji  razvoj  lekova. Osvetljen  je  antimikrobni  karakter Phomopsis vrsta, a takođe su procenjeni i budući pravci u njegovoj terapijskoj primeni.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za farmaciju",
title = "Chemometric window to antimicrobial activity of biomolecules isolated from endophytic fungi, Hemometrijski prozor ka antibakterijskoj aktivnosti biomolekula izolovanih iz endofitnih gljiva",
volume = "70",
number = "3",
pages = "142-156",
doi = "10.5937/arhfarm2003142O"
}

Otašević, B., Krmar, J., Đajić, N., Ignjatović, J.,& Protić, A.. (2020). Chemometric window to antimicrobial activity of biomolecules isolated from endophytic fungi. in Arhiv za farmaciju
Pharmaceutical Association of Serbia., 70(3), 142-156.
https://doi.org/10.5937/arhfarm2003142O

Otašević B, Krmar J, Đajić N, Ignjatović J, Protić A. Chemometric window to antimicrobial activity of biomolecules isolated from endophytic fungi. in Arhiv za farmaciju. 2020;70(3):142-156.
doi:10.5937/arhfarm2003142O .

Otašević, Biljana, Krmar, Jovana, Đajić, Nevena, Ignjatović, Janko, Protić, Ana, "Chemometric window to antimicrobial activity of biomolecules isolated from endophytic fungi" in Arhiv za farmaciju, 70, no. 3 (2020):142-156,
https://doi.org/10.5937/arhfarm2003142O . .

TY  - JOUR
AU  - Ignjatović, Janko
AU  - Maljurić, Nevena
AU  - Golubović, Jelena
AU  - Ravnikar, Matjaž
AU  - Petković, Miloš
AU  - Savodnik, Nika
AU  - Štrukelj, Borut
AU  - Otašević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3661
AB  - Recently, growing interest is devoted to investigation of bioactive secondary metabolites of endophytic fungi. Thus, as an extension to our previous achievements related to antimicrobial potential of endophytic fungi, Phomopsis species isolated from  conifer  needles  was  selected  as  appropriately  promising  natural  source  for  drug  discovery.  Its  dichloromethane  and  ethanol  extracts  considerably  inhibited  growth  of  Escherichia  coli  and  Staphylococcus  aureus.  Moreover,  the  indi-vidual compounds of dichloromethane extract have been separated, collected and purified using semi preparative liquid chromatographic analysis and comprehensively characterized using mass spectrometry (MS) and nuclear magnetic res-onance spectroscopy (NMR). Based on their antimicrobial activity and unique structural characteristics in comparison with  well-established  drugs  from  the  same  therapeutic  category,  two  dominant  compounds  (Z)-(Z)-2-acetoxyprop-1-en-1-yl-3-(3-((E)-3,4-dihydroxypent-1-en-1-yl)oxiran-2-yl)acrylate  (denoted  as  325-3)  and  (Z)-(Z)-2-acetoxyprop-1-en-1-yl  3-(3-((E)-4-hydroxy-3-oxopent-1-en-1-yl)oxiran-2-yl)acrylate  (denoted  as  325-5)  were  recognized  as  valuable  leading structures for future discovery of novel antibiotics.
PB  - Slovenian Chemical Society
T2  - Acta Chimica Slovenica
T1  - Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species
VL  - 67
IS  - 2
SP  - 445
EP  - 461
DO  - 10.17344/acsi.2019.5389
ER  - 

@article{
author = "Ignjatović, Janko and Maljurić, Nevena and Golubović, Jelena and Ravnikar, Matjaž and Petković, Miloš and Savodnik, Nika and Štrukelj, Borut and Otašević, Biljana",
year = "2020",
abstract = "Recently, growing interest is devoted to investigation of bioactive secondary metabolites of endophytic fungi. Thus, as an extension to our previous achievements related to antimicrobial potential of endophytic fungi, Phomopsis species isolated from  conifer  needles  was  selected  as  appropriately  promising  natural  source  for  drug  discovery.  Its  dichloromethane  and  ethanol  extracts  considerably  inhibited  growth  of  Escherichia  coli  and  Staphylococcus  aureus.  Moreover,  the  indi-vidual compounds of dichloromethane extract have been separated, collected and purified using semi preparative liquid chromatographic analysis and comprehensively characterized using mass spectrometry (MS) and nuclear magnetic res-onance spectroscopy (NMR). Based on their antimicrobial activity and unique structural characteristics in comparison with  well-established  drugs  from  the  same  therapeutic  category,  two  dominant  compounds  (Z)-(Z)-2-acetoxyprop-1-en-1-yl-3-(3-((E)-3,4-dihydroxypent-1-en-1-yl)oxiran-2-yl)acrylate  (denoted  as  325-3)  and  (Z)-(Z)-2-acetoxyprop-1-en-1-yl  3-(3-((E)-4-hydroxy-3-oxopent-1-en-1-yl)oxiran-2-yl)acrylate  (denoted  as  325-5)  were  recognized  as  valuable  leading structures for future discovery of novel antibiotics.",
publisher = "Slovenian Chemical Society",
journal = "Acta Chimica Slovenica",
title = "Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species",
volume = "67",
number = "2",
pages = "445-461",
doi = "10.17344/acsi.2019.5389"
}

Ignjatović, J., Maljurić, N., Golubović, J., Ravnikar, M., Petković, M., Savodnik, N., Štrukelj, B.,& Otašević, B.. (2020). Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species. in Acta Chimica Slovenica
Slovenian Chemical Society., 67(2), 445-461.
https://doi.org/10.17344/acsi.2019.5389

Ignjatović J, Maljurić N, Golubović J, Ravnikar M, Petković M, Savodnik N, Štrukelj B, Otašević B. Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species. in Acta Chimica Slovenica. 2020;67(2):445-461.
doi:10.17344/acsi.2019.5389 .

Ignjatović, Janko, Maljurić, Nevena, Golubović, Jelena, Ravnikar, Matjaž, Petković, Miloš, Savodnik, Nika, Štrukelj, Borut, Otašević, Biljana, "Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species" in Acta Chimica Slovenica, 67, no. 2 (2020):445-461,
https://doi.org/10.17344/acsi.2019.5389 . .

TY  - CONF
AU  - Otašević, Biljana
AU  - Ignjatović, Janko
AU  - Maljurić, Nevena
AU  - Krmar, Jovana
AU  - Zečević, Mira
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4701
PB  - BIT Group Global Ltd.
C3  - The 7th Annual conference of AnalytiX-2019 (Europe), 13-15. November 2019., Berlin, Germany
T1  - Chemometric Approach in Bioactivity Profiling – The Case of Biomolecules from Endophytic Fungi
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4701
ER  - 

@conference{
author = "Otašević, Biljana and Ignjatović, Janko and Maljurić, Nevena and Krmar, Jovana and Zečević, Mira",
year = "2019",
publisher = "BIT Group Global Ltd.",
journal = "The 7th Annual conference of AnalytiX-2019 (Europe), 13-15. November 2019., Berlin, Germany",
title = "Chemometric Approach in Bioactivity Profiling – The Case of Biomolecules from Endophytic Fungi",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4701"
}

Otašević, B., Ignjatović, J., Maljurić, N., Krmar, J.,& Zečević, M.. (2019). Chemometric Approach in Bioactivity Profiling – The Case of Biomolecules from Endophytic Fungi. in The 7th Annual conference of AnalytiX-2019 (Europe), 13-15. November 2019., Berlin, Germany
BIT Group Global Ltd...
https://hdl.handle.net/21.15107/rcub_farfar_4701

Otašević B, Ignjatović J, Maljurić N, Krmar J, Zečević M. Chemometric Approach in Bioactivity Profiling – The Case of Biomolecules from Endophytic Fungi. in The 7th Annual conference of AnalytiX-2019 (Europe), 13-15. November 2019., Berlin, Germany. 2019;.
https://hdl.handle.net/21.15107/rcub_farfar_4701 .

Otašević, Biljana, Ignjatović, Janko, Maljurić, Nevena, Krmar, Jovana, Zečević, Mira, "Chemometric Approach in Bioactivity Profiling – The Case of Biomolecules from Endophytic Fungi" in The 7th Annual conference of AnalytiX-2019 (Europe), 13-15. November 2019., Berlin, Germany (2019),
https://hdl.handle.net/21.15107/rcub_farfar_4701 .

TY  - JOUR
AU  - Marković, Bojan
AU  - Ignjatović, Janko
AU  - Vujadinović, Mirjana
AU  - Savić, Vedrana
AU  - Vladimirov, Sote
AU  - Karljiković-Rajić, Katarina
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2323
AB  - Inter-laboratory verification of European pharmacopoeia (EP) monograph on derivative spectrophotometry (DS) method and its application for chitosan hydrochloride was carried out on two generation of instruments (earlier GBC Cintra 20 and current technology TS Evolution 300). Instruments operate with different versions of Savitzky-Golay algorithm and modes of generating digital derivative spectra. For resolution power parameter, defined as the amplitude ratio A/B in DS method EP monograph, comparable results were obtained only with algorithm's parameters smoothing points (SP) 7 and the 2nd degree polynomial and those provided corresponding data with other two modes on TS Evolution 300 Medium digital indirect and Medium digital direct. Using quoted algorithm's parameters, the differences in percentages between the amplitude ratio A/B averages, were within accepted criteria (+/- 13%) for assay of drug product for method transfer. The deviation of 1.76% for the degree of deacetylation assessment of chitosan hydrochloride, determined on two instruments, (amplitude D-1(202); the 2nd degree polynomial and SP 9 in Savitzky-Golay algorithm), was acceptable, since it was within allowed criteria (+/- 2%) for assay deviation of drug substance, for method transfer in pharmaceutical analyses.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectroscopy Letters
T1  - Inter-laboratory verification of European pharmacopoeia monograph on derivative spectrophotometry method and its application for chitosan hydrochloride
VL  - 150
SP  - 792
EP  - 798
DO  - 10.1016/j.saa.2015.06.022
ER  - 

@article{
author = "Marković, Bojan and Ignjatović, Janko and Vujadinović, Mirjana and Savić, Vedrana and Vladimirov, Sote and Karljiković-Rajić, Katarina",
year = "2015",
abstract = "Inter-laboratory verification of European pharmacopoeia (EP) monograph on derivative spectrophotometry (DS) method and its application for chitosan hydrochloride was carried out on two generation of instruments (earlier GBC Cintra 20 and current technology TS Evolution 300). Instruments operate with different versions of Savitzky-Golay algorithm and modes of generating digital derivative spectra. For resolution power parameter, defined as the amplitude ratio A/B in DS method EP monograph, comparable results were obtained only with algorithm's parameters smoothing points (SP) 7 and the 2nd degree polynomial and those provided corresponding data with other two modes on TS Evolution 300 Medium digital indirect and Medium digital direct. Using quoted algorithm's parameters, the differences in percentages between the amplitude ratio A/B averages, were within accepted criteria (+/- 13%) for assay of drug product for method transfer. The deviation of 1.76% for the degree of deacetylation assessment of chitosan hydrochloride, determined on two instruments, (amplitude D-1(202); the 2nd degree polynomial and SP 9 in Savitzky-Golay algorithm), was acceptable, since it was within allowed criteria (+/- 2%) for assay deviation of drug substance, for method transfer in pharmaceutical analyses.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectroscopy Letters",
title = "Inter-laboratory verification of European pharmacopoeia monograph on derivative spectrophotometry method and its application for chitosan hydrochloride",
volume = "150",
pages = "792-798",
doi = "10.1016/j.saa.2015.06.022"
}

Marković, B., Ignjatović, J., Vujadinović, M., Savić, V., Vladimirov, S.,& Karljiković-Rajić, K.. (2015). Inter-laboratory verification of European pharmacopoeia monograph on derivative spectrophotometry method and its application for chitosan hydrochloride. in Spectroscopy Letters
Pergamon-Elsevier Science Ltd, Oxford., 150, 792-798.
https://doi.org/10.1016/j.saa.2015.06.022

Marković B, Ignjatović J, Vujadinović M, Savić V, Vladimirov S, Karljiković-Rajić K. Inter-laboratory verification of European pharmacopoeia monograph on derivative spectrophotometry method and its application for chitosan hydrochloride. in Spectroscopy Letters. 2015;150:792-798.
doi:10.1016/j.saa.2015.06.022 .

Marković, Bojan, Ignjatović, Janko, Vujadinović, Mirjana, Savić, Vedrana, Vladimirov, Sote, Karljiković-Rajić, Katarina, "Inter-laboratory verification of European pharmacopoeia monograph on derivative spectrophotometry method and its application for chitosan hydrochloride" in Spectroscopy Letters, 150 (2015):792-798,
https://doi.org/10.1016/j.saa.2015.06.022 . .