@conference{
author = "Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "Chamaecytisus heuffelii subsp. jankae (Velen.) Niketić (Fabaceae) is a dwarfish shrub,
which range includes eastern part of the Balkan Peninsula (Albania, Bulgaria, Serbia) (1).
The aim of the investigation was to chemically characterize aerial flowering parts of this
plant: the dried methanol extract (TM), its alkaloid (AF) and non-alkaloid (NAF) fractions,
and to assess their ability and the ability of detected flavonoids to inhibit enzymes
acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), in vitro in the Ellman
spectrophotometric test. Plant material was collected in eastern Serbia (Jelašnička Klisura
gorge), dried, powdered and extracted with methanol, after dichloromethane pre-extraction.
Using solvents of different polarities and pH value, AF (yield 14.35%) and NAF (yield
57.78%) were obtained from TM. By LC-MS analysis, flavonoids luteolin-8-C-
glucosylpentoside (159.81 mg/g), genistin (22.38 mg/g), vitexin (6.32 mg/g), rutin (4.25
mg/g), and genistein (0.86 mg/g) were detected and quantified in TM, while luteolin-8-C-
glucosylpentoside (212.70 mg/g), rutin (1.38 mg/g), and genistin (1.03 mg/g) in NAF. Using
GC-FID-MS analysis, quinolizidine alkaloids sparteine, 17-oksosparteine, and lupanine were
identified in AF. TM and NAF exhibited significant and comparable anti-cholinesterase
activity (IC50AChE 0.54 and 0.72 mg/mL; IC50BuChE 0.45 and 0.68 mg/mL). All detected
flavonoids evinced the ability to inhibit enzymes; the most active were genistein and vitexin
(IC50AChE 54.75 and 88.35 μg/mL; IC 50BuChE 27.83 and 49.77 μg/mL). Considering the
quinolizidine alkaloids toxicity and AF poor anti-cholinesterase activity, it might be
concluded, regarding safety profile and medical potential, that the fraction of the herb
methanol extract, flavonoid-rich and alkaloid-free (NAF), is good candidate for further
research., Chamaecytisus heuffelii subsp. jankae (Velen.) Niketić (Fabaceae) je polužbun, koji
raste na području istočnog dela Balkanskog poluostrva (Albanija, Bugarska, Srbija) (1). Cilj
istraživanja bio je da se izvrši hemijska karakterizacija nadzemnih delova u cvetu ove biljke:
suvog metanolnog ekstrakta (TM), njegove alkaloidne (AF) i frakcije bez alkaloida (NAF), i da
se in vitro, u Ellmanov-om spektrofotomerijskom testu, ispita njihova sposobnost, kao i
sposobnost detektovanih flavonoida da inhibiraju enzime acetilholinesterazu (AChE) i
butirilholinesterazu (BChE). Biljni materijal je sakupljen u istočnoj Srbiji (Jelašnička klisura),
osušen, samleven i ekstrahovan metanolom, nakon pre-ekstrakcije dihlormetanom.
Primenom rastvarača različite polarnosti uz promenu pH vrednosti, iz TM su dobijene AF
(prinos 14,35%) i NAF (prinos 57,78%). LC-MS analizom, u TM detektovani su i
kvanitikovani flavonoidi luteolin-8-C-glukozilpentozid (159,81 mg/g), genistin (22,38 mg/g),
viteksin (6,32 mg/g) i rutin (4,25 mg/g) i genistein (0,86 mg/g), a u NAF luteolin-8-C-
glukozilpentozid (212,70 mg/g), rutin (1,38 mg/g) i genistin (1,03 mg/g). GC-FID-MS
analizom, u AF identifikovani su hinolizidinski alkaloidi spartein, 17-oksospartein i lupanin.
TM i NAF ispoljili su značajnu i uporedivu antiholinesteraznu aktivnost (IC50AChE 0,54 i 0,72
mg/mL; IC 50BChE 0,45 i 0,68 mg/mL). Svi detektovani flavonoidi su pokazali sposobnost da
inhibiraju enzime; najaktivniji su bili genistein i viteksin (IC 50AChE 54,75 i 88,35 μg/mL;
IC 50BuChE 27,83 i 49,77 μg/mL). Uzimajući u obzir toksič nost hinolizidinskih alkaloida i slabu
antiholinesteraznu aktivnost AF, mož e se zaključ iti da je frakcija metanolnog ekstrakata
herbe bogata flavonoidima i oslobođena alkaloida (NAF), u pogledu bezbednosnog profila i
lekovitog potencijala dobar kandidat za dalja ispitivanja.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Secondary metabolites and anti-cholinesterase activity of Chamaecytisus heuffelii subsp. jankae aerial flowering parts, Sekundarni metaboliti i antiholinesterazna aktivnost herbe Chamaecytisus heuffelii subsp. jankae",
volume = "72",
number = "4 suplement",
pages = "S471-S472",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4585"
}