TY  - JOUR
AU  - Uroić, Ksenija
AU  - Nikolić, Milica
AU  - Kos, Blazenka
AU  - Lebos-Pavunc, Andreja
AU  - Beganović, Jasna
AU  - Lukić, Jovanka
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Miljković, Marija
AU  - Golić, Nataša
AU  - Topisirović, Ljubiša
AU  - Cadez, Neza
AU  - Raspor, Peter
AU  - Susković, Jagoda
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2197
AB  - The aim of this study is to gain insight into the probiotic potential of autochthonous lactic acid bacteria (LAB) isolated from artisanal fresh soft and white pickled cheeses. Eleven out of 86 LAB isolates from traditionally produced artisanal fresh soft and white pickled cheeses which survived the most rigorous simulated gastrointestinal tract conditions and did not show resistance to antibiotics were subjected to further evaluation for functional probiotic properties. The ability of the examined strains to assimilate cholesterol in the presence of bile salts was strain dependent, with the highest percentage of cholesterol assimilated by strain Lactobacillus brevis BGGO7-28 possessing S-layer proteins on its cell surface. The growth of strains with mannitol or lactulose as the only carbon source was better than with fructooligosaccharides (FOS) and inulin as prebiotic substrates, which should be considered in the production of synbiotics. Moreover, the results demonstrated that the strains were highly adhesive to human enterocyte-like Caco-2 cells and to a lesser extent to HT29-MTX cells, with the exception of strain Lb. brevis BGGO7-28, which showed similar percentage of adhesion to both cell lines. This strain was the only one with the acidic cell surface, while other examined strains have the cell surfaces with electron donor and basic properties. In addition, all selected strains decreased the proliferation of gut-associated lymphoid tissue (GALT) cells, suggesting possible immunomodulatory potential of the isolates. Finally, the number of viable cells in dry active preparations after lyophilisation depended on the lyoprotectant used (inulin, FOS or skimmed milk), as well as on the strain subjected to lyophilisation. In conclusion, the results obtained in this study demonstrate that particular dairy LAB isolates exhibit strain-specific probiotic properties. Thus, they could be further examined as part of mixed autochthonous starter cultures for traditional cheese production under controlled conditions.
PB  - Faculty Food Technology Biotechnology, Zagreb
T2  - Food Technology and Biotechnology
T1  - Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese
VL  - 52
IS  - 2
SP  - 232
EP  - 241
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2197
ER  - 

@article{
author = "Uroić, Ksenija and Nikolić, Milica and Kos, Blazenka and Lebos-Pavunc, Andreja and Beganović, Jasna and Lukić, Jovanka and Jovčić, Branko and Filipić, Brankica and Miljković, Marija and Golić, Nataša and Topisirović, Ljubiša and Cadez, Neza and Raspor, Peter and Susković, Jagoda",
year = "2014",
abstract = "The aim of this study is to gain insight into the probiotic potential of autochthonous lactic acid bacteria (LAB) isolated from artisanal fresh soft and white pickled cheeses. Eleven out of 86 LAB isolates from traditionally produced artisanal fresh soft and white pickled cheeses which survived the most rigorous simulated gastrointestinal tract conditions and did not show resistance to antibiotics were subjected to further evaluation for functional probiotic properties. The ability of the examined strains to assimilate cholesterol in the presence of bile salts was strain dependent, with the highest percentage of cholesterol assimilated by strain Lactobacillus brevis BGGO7-28 possessing S-layer proteins on its cell surface. The growth of strains with mannitol or lactulose as the only carbon source was better than with fructooligosaccharides (FOS) and inulin as prebiotic substrates, which should be considered in the production of synbiotics. Moreover, the results demonstrated that the strains were highly adhesive to human enterocyte-like Caco-2 cells and to a lesser extent to HT29-MTX cells, with the exception of strain Lb. brevis BGGO7-28, which showed similar percentage of adhesion to both cell lines. This strain was the only one with the acidic cell surface, while other examined strains have the cell surfaces with electron donor and basic properties. In addition, all selected strains decreased the proliferation of gut-associated lymphoid tissue (GALT) cells, suggesting possible immunomodulatory potential of the isolates. Finally, the number of viable cells in dry active preparations after lyophilisation depended on the lyoprotectant used (inulin, FOS or skimmed milk), as well as on the strain subjected to lyophilisation. In conclusion, the results obtained in this study demonstrate that particular dairy LAB isolates exhibit strain-specific probiotic properties. Thus, they could be further examined as part of mixed autochthonous starter cultures for traditional cheese production under controlled conditions.",
publisher = "Faculty Food Technology Biotechnology, Zagreb",
journal = "Food Technology and Biotechnology",
title = "Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese",
volume = "52",
number = "2",
pages = "232-241",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2197"
}

Uroić, K., Nikolić, M., Kos, B., Lebos-Pavunc, A., Beganović, J., Lukić, J., Jovčić, B., Filipić, B., Miljković, M., Golić, N., Topisirović, L., Cadez, N., Raspor, P.,& Susković, J.. (2014). Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese. in Food Technology and Biotechnology
Faculty Food Technology Biotechnology, Zagreb., 52(2), 232-241.
https://hdl.handle.net/21.15107/rcub_farfar_2197

Uroić K, Nikolić M, Kos B, Lebos-Pavunc A, Beganović J, Lukić J, Jovčić B, Filipić B, Miljković M, Golić N, Topisirović L, Cadez N, Raspor P, Susković J. Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese. in Food Technology and Biotechnology. 2014;52(2):232-241.
https://hdl.handle.net/21.15107/rcub_farfar_2197 .

Uroić, Ksenija, Nikolić, Milica, Kos, Blazenka, Lebos-Pavunc, Andreja, Beganović, Jasna, Lukić, Jovanka, Jovčić, Branko, Filipić, Brankica, Miljković, Marija, Golić, Nataša, Topisirović, Ljubiša, Cadez, Neza, Raspor, Peter, Susković, Jagoda, "Probiotic Properties of Lactic Acid Bacteria Isolated from Croatian Fresh Soft Cheese and Serbian White Pickled Cheese" in Food Technology and Biotechnology, 52, no. 2 (2014):232-241,
https://hdl.handle.net/21.15107/rcub_farfar_2197 .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Jovčić, Branko
AU  - Uzelac, Gordana
AU  - Miljković, Marija
AU  - Antić-Stanković, Jelena
AU  - Topisirović, Ljubiša
AU  - Golić, Nataša
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2045
AB  - The influence of the over-expression of CmbT multidrug resistance transporter on the growth rate of Lactococcus lactis NZ9000 was studied. L. lactis is a lactic acid bacteria (LAB) widely used as a starter culture in dairy industry. Recently characterized CmbT MDR transporter in L. lactis confers resistance to a wide variety of toxic compounds as well as to some clinically relevant antibiotics. In this study, the cmbT gene was over-expressed in the strain L. lactis NZ9000 in the presence of nisin inducer. Over-expression of the cmbT gene in L. lactis NZ9000 was followed by RT-PCR. The obtained results showed that the cmbT gene was successfully over-expressed by addition of sub-inhibitory amounts of nisin. Growth curves of L. lactis NZ9000/pCT50 over-expressing the cmbT gene and L. lactis NZ9000 control strain were followed in the rich medium as well as in the chemically defined medium in the presence solely of methionine (0.084 mM) or mix of methionine and cysteine (8.4 mM and 8.2 mM, respectively). Resulting doubling times revealed that L. lactis NZ9000/pCT50 had higher growth rate comparing to the control strain. This could be a consequence of the CmbT efflux activity, which improves the fitness of the host bacterium through the elimination of toxic compounds from the cell.
AB  - U ovom radu je izučavan uticaj povećane ekspresije cmbT gena, odgovornog za sintezu CmbT MDR transportera, na rast Lactococcus lactis. L. lactis pripada grupi bakterija mlečne kiseline (BMK) i ima veliku primenu u prehrambenoj industriji kao starter kultura. CmbT transporter je nedavno okarakterisan MDR protein soja L. lactis, koji doprinosi rezistenciji na različite toksične agense kao i na neke klinički značajne antibiotike. U ovom radu je cmbT gen višestruko eksprimiran u soju L. lactis NZ9000 dodavanjem nizina kao inducera. Povećana ekspresija cmbT gena je praćena metodom reverzne transkripcije (RT-PCR). Pokazano je da se nakon dodatka subinhibitornih koncentracija nizina u medijum za rast povećava količina sintetisane informacione RNK specifične za cmbT gen. Rast soja L. lactis NZ9000/pCT50, u kome je višestruko eksprimiran cmbT gen i L. lactis NZ9000 kontrolnog soja praćen je u bogatom i hemijski definisanom medijumu u prisustvu samo metionina (0.084 mM) ili kombinacije metionina i cisteina (8.4 mM i 8.2 mM). Praćene su krive rasta oba soja, a nakon izračunavanja odgovarajućih vremena generacije, rezultati su pokazali da L. lactis NZ9000/pCT50, brže raste u odnosu na kontrolni soj. Uočena razlika je najverovatnije posledica aktivnosti CmbT transportera koji doprinosi izbacivanju toksičnih agenasa iz ćelije i na taj način poboljšava adaptivne sposobnosti bakterije koja ga eksprimira i daje joj selektivnu prednost.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika, Belgrade
T1  - Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis
T1  - Uticaj povećane ekspresije CmbT MDR transportera na rast Lactococcus lactis
VL  - 45
IS  - 1
SP  - 197
EP  - 206
DO  - 10.2298/GENSR1301197F
ER  - 

@article{
author = "Filipić, Brankica and Jovčić, Branko and Uzelac, Gordana and Miljković, Marija and Antić-Stanković, Jelena and Topisirović, Ljubiša and Golić, Nataša",
year = "2013",
abstract = "The influence of the over-expression of CmbT multidrug resistance transporter on the growth rate of Lactococcus lactis NZ9000 was studied. L. lactis is a lactic acid bacteria (LAB) widely used as a starter culture in dairy industry. Recently characterized CmbT MDR transporter in L. lactis confers resistance to a wide variety of toxic compounds as well as to some clinically relevant antibiotics. In this study, the cmbT gene was over-expressed in the strain L. lactis NZ9000 in the presence of nisin inducer. Over-expression of the cmbT gene in L. lactis NZ9000 was followed by RT-PCR. The obtained results showed that the cmbT gene was successfully over-expressed by addition of sub-inhibitory amounts of nisin. Growth curves of L. lactis NZ9000/pCT50 over-expressing the cmbT gene and L. lactis NZ9000 control strain were followed in the rich medium as well as in the chemically defined medium in the presence solely of methionine (0.084 mM) or mix of methionine and cysteine (8.4 mM and 8.2 mM, respectively). Resulting doubling times revealed that L. lactis NZ9000/pCT50 had higher growth rate comparing to the control strain. This could be a consequence of the CmbT efflux activity, which improves the fitness of the host bacterium through the elimination of toxic compounds from the cell., U ovom radu je izučavan uticaj povećane ekspresije cmbT gena, odgovornog za sintezu CmbT MDR transportera, na rast Lactococcus lactis. L. lactis pripada grupi bakterija mlečne kiseline (BMK) i ima veliku primenu u prehrambenoj industriji kao starter kultura. CmbT transporter je nedavno okarakterisan MDR protein soja L. lactis, koji doprinosi rezistenciji na različite toksične agense kao i na neke klinički značajne antibiotike. U ovom radu je cmbT gen višestruko eksprimiran u soju L. lactis NZ9000 dodavanjem nizina kao inducera. Povećana ekspresija cmbT gena je praćena metodom reverzne transkripcije (RT-PCR). Pokazano je da se nakon dodatka subinhibitornih koncentracija nizina u medijum za rast povećava količina sintetisane informacione RNK specifične za cmbT gen. Rast soja L. lactis NZ9000/pCT50, u kome je višestruko eksprimiran cmbT gen i L. lactis NZ9000 kontrolnog soja praćen je u bogatom i hemijski definisanom medijumu u prisustvu samo metionina (0.084 mM) ili kombinacije metionina i cisteina (8.4 mM i 8.2 mM). Praćene su krive rasta oba soja, a nakon izračunavanja odgovarajućih vremena generacije, rezultati su pokazali da L. lactis NZ9000/pCT50, brže raste u odnosu na kontrolni soj. Uočena razlika je najverovatnije posledica aktivnosti CmbT transportera koji doprinosi izbacivanju toksičnih agenasa iz ćelije i na taj način poboljšava adaptivne sposobnosti bakterije koja ga eksprimira i daje joj selektivnu prednost.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika, Belgrade",
title = "Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis, Uticaj povećane ekspresije CmbT MDR transportera na rast Lactococcus lactis",
volume = "45",
number = "1",
pages = "197-206",
doi = "10.2298/GENSR1301197F"
}

Filipić, B., Jovčić, B., Uzelac, G., Miljković, M., Antić-Stanković, J., Topisirović, L.,& Golić, N.. (2013). Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis. in Genetika, Belgrade
Društvo genetičara Srbije, Beograd., 45(1), 197-206.
https://doi.org/10.2298/GENSR1301197F

Filipić B, Jovčić B, Uzelac G, Miljković M, Antić-Stanković J, Topisirović L, Golić N. Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis. in Genetika, Belgrade. 2013;45(1):197-206.
doi:10.2298/GENSR1301197F .

Filipić, Brankica, Jovčić, Branko, Uzelac, Gordana, Miljković, Marija, Antić-Stanković, Jelena, Topisirović, Ljubiša, Golić, Nataša, "Over-expressed CmbT multidrug resistance transporter improves the fitness of Lactococcus lactis" in Genetika, Belgrade, 45, no. 1 (2013):197-206,
https://doi.org/10.2298/GENSR1301197F . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Golić, Nataša
AU  - Jovčić, Branko
AU  - Tolinacki, Maja
AU  - Bay, Denice C.
AU  - Turner, Raymond J.
AU  - Antić-Stanković, Jelena
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1944
AB  - Functional characterization of the multidrug resistance CmbT transporter was performed in Lactococcus lactis. The cmbT gene is predicted to encode an efflux protein homologous to the multidrug resistance major facilitator superfamily. The cmbT gene (1377 bp) was cloned and overexpressed in L. lactis NZ9000. Results from cell growth studies revealed that the CmbT protein has an effect on host cell resistance to lincomycin, cholate, sulbactam, ethidium bromide, Hoechst 33342, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametoxazole. Moreover, in vivo transport assays showed that overexpressed CmbT-mediated extrusion of ethidium bromide and Hoechst 33342 was higher than in the control L. lactis NZ9000 strain. CmbT-mediated extrusion of Hoechst 33342 was inhibited by the ionophores nigericin and valinomycin known to dissipate proton motive force. This indicates that CmbT-mediated extrusion is based on a drug-proton antiport mechanism. Taking together results obtained in this study, it can be concluded that CmbT is a novel major facilitator multidrug resistance transporter candidate in L. lactis, with a possible signaling role in sulfur metabolism.
PB  - Elsevier Science BV, Amsterdam
T2  - Research in Social & Administrative Pharmacy
T1  - The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis
VL  - 164
IS  - 1
SP  - 46
EP  - 54
DO  - 10.1016/j.resmic.2012.09.003
ER  - 

@article{
author = "Filipić, Brankica and Golić, Nataša and Jovčić, Branko and Tolinacki, Maja and Bay, Denice C. and Turner, Raymond J. and Antić-Stanković, Jelena and Kojić, Milan and Topisirović, Ljubiša",
year = "2013",
abstract = "Functional characterization of the multidrug resistance CmbT transporter was performed in Lactococcus lactis. The cmbT gene is predicted to encode an efflux protein homologous to the multidrug resistance major facilitator superfamily. The cmbT gene (1377 bp) was cloned and overexpressed in L. lactis NZ9000. Results from cell growth studies revealed that the CmbT protein has an effect on host cell resistance to lincomycin, cholate, sulbactam, ethidium bromide, Hoechst 33342, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametoxazole. Moreover, in vivo transport assays showed that overexpressed CmbT-mediated extrusion of ethidium bromide and Hoechst 33342 was higher than in the control L. lactis NZ9000 strain. CmbT-mediated extrusion of Hoechst 33342 was inhibited by the ionophores nigericin and valinomycin known to dissipate proton motive force. This indicates that CmbT-mediated extrusion is based on a drug-proton antiport mechanism. Taking together results obtained in this study, it can be concluded that CmbT is a novel major facilitator multidrug resistance transporter candidate in L. lactis, with a possible signaling role in sulfur metabolism.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Research in Social & Administrative Pharmacy",
title = "The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis",
volume = "164",
number = "1",
pages = "46-54",
doi = "10.1016/j.resmic.2012.09.003"
}

Filipić, B., Golić, N., Jovčić, B., Tolinacki, M., Bay, D. C., Turner, R. J., Antić-Stanković, J., Kojić, M.,& Topisirović, L.. (2013). The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis. in Research in Social & Administrative Pharmacy
Elsevier Science BV, Amsterdam., 164(1), 46-54.
https://doi.org/10.1016/j.resmic.2012.09.003

Filipić B, Golić N, Jovčić B, Tolinacki M, Bay DC, Turner RJ, Antić-Stanković J, Kojić M, Topisirović L. The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis. in Research in Social & Administrative Pharmacy. 2013;164(1):46-54.
doi:10.1016/j.resmic.2012.09.003 .

Filipić, Brankica, Golić, Nataša, Jovčić, Branko, Tolinacki, Maja, Bay, Denice C., Turner, Raymond J., Antić-Stanković, Jelena, Kojić, Milan, Topisirović, Ljubiša, "The cmbT gene encodes a novel major facilitator multidrug resistance transporter in Lactococcus lactis" in Research in Social & Administrative Pharmacy, 164, no. 1 (2013):46-54,
https://doi.org/10.1016/j.resmic.2012.09.003 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Strahinić, Ivana
AU  - Milenković, Marina
AU  - Golić, Nataša
AU  - Kojić, Milan
AU  - Topisirović, Ljubiša
AU  - Begović, Jelena
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1953
AB  - The present study was carried out to test the colonic mucosal response of rats to oral supplementation with Lactobacillus fermentum BGHI14 and to correlate the tissue reaction to trinitrobenzenesulfonate (TNBS)-induced colitis with mucosal barrier alterations caused by bacterial ingestion. An immune cell-mediated reaction of healthy colonic tissue was noticed after bacterial feeding. After prolonged bacterial treatment, the observed reaction had retreated to normality, but the mRNA levels of proinflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) remained elevated. These data point to the chronic low-grade inflammation that could be caused by long-term probiotic consumption. Although no detrimental effects of bacterial pretreatment were noticed in colitic rats, at least in the acute state of disease, the results obtained in our study point to the necessity of reassessment of existing data on the safety of probiotic preparations. Additionally, probiotic effects in experimental colitis models might depend on time coordination of disease induction with treatment duration.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction
VL  - 79
IS  - 18
SP  - 5735
EP  - 5744
DO  - 10.1128/AEM.01807-13
ER  - 

@article{
author = "Lukić, Jovanka and Strahinić, Ivana and Milenković, Marina and Golić, Nataša and Kojić, Milan and Topisirović, Ljubiša and Begović, Jelena",
year = "2013",
abstract = "The present study was carried out to test the colonic mucosal response of rats to oral supplementation with Lactobacillus fermentum BGHI14 and to correlate the tissue reaction to trinitrobenzenesulfonate (TNBS)-induced colitis with mucosal barrier alterations caused by bacterial ingestion. An immune cell-mediated reaction of healthy colonic tissue was noticed after bacterial feeding. After prolonged bacterial treatment, the observed reaction had retreated to normality, but the mRNA levels of proinflammatory cytokines interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF-alpha) remained elevated. These data point to the chronic low-grade inflammation that could be caused by long-term probiotic consumption. Although no detrimental effects of bacterial pretreatment were noticed in colitic rats, at least in the acute state of disease, the results obtained in our study point to the necessity of reassessment of existing data on the safety of probiotic preparations. Additionally, probiotic effects in experimental colitis models might depend on time coordination of disease induction with treatment duration.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction",
volume = "79",
number = "18",
pages = "5735-5744",
doi = "10.1128/AEM.01807-13"
}

Lukić, J., Strahinić, I., Milenković, M., Golić, N., Kojić, M., Topisirović, L.,& Begović, J.. (2013). Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 79(18), 5735-5744.
https://doi.org/10.1128/AEM.01807-13

Lukić J, Strahinić I, Milenković M, Golić N, Kojić M, Topisirović L, Begović J. Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction. in Applied and Environmental Microbiology. 2013;79(18):5735-5744.
doi:10.1128/AEM.01807-13 .

Lukić, Jovanka, Strahinić, Ivana, Milenković, Marina, Golić, Nataša, Kojić, Milan, Topisirović, Ljubiša, Begović, Jelena, "Interaction of Lactobacillus fermentum BGHI14 with Rat Colonic Mucosa: Implications for Colitis Induction" in Applied and Environmental Microbiology, 79, no. 18 (2013):5735-5744,
https://doi.org/10.1128/AEM.01807-13 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Strahinić, Ivana
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Topisirović, Ljubiša
AU  - Kojić, Milan
AU  - Begović, Jelena
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1629
AB  - Adhesion of bacteria to mucosal surfaces and epithelial cells is one of the key features for the selection of probiotics. In this study, we assessed the adhesion property of Lactococcus lactis subsp. lactis BGKP1 based on its strong autoaggregation phenotype and the presence of the mucin binding protein (MbpL). Genes involved in aggregation (aggL) and possible interaction with mucin (mbpL), present on the same plasmid pKP1, were previously separately cloned in the plasmid pAZIL. In vivo and in vitro experiments revealed potentially different physiological roles of these two proteins in the process of adherence to the intestine during the passage of the strain through the gastrointestinal tract. We correlated the in vitro and in vivo aggregation of the BGKP1-20 carrying plasmid with aggL to binding to the colonic mucus through nonspecific hydrophobic interactions. The expression of AggL on the bacterial cell surface significantly increased the hydrophobicity of the strain. On the other hand, the presence of AggL in the strain reduced its ability to adhere to the ileum. Moreover, MbpL protein showed an affinity to bind gastric type mucin proteins such as MUC5AC. This protein did not contribute to the binding of the strain to the ileal or colonic part of the intestine. Different potential functions of lactococcal AggL and MbpL proteins in the process of adhesion to the gastrointestinal tract are proposed.
PB  - Amer Soc Microbiology, Washington
T2  - Applied and Environmental Microbiology
T1  - Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa
VL  - 78
IS  - 22
SP  - 7993
EP  - 8000
DO  - 10.1128/AEM.02141-12
ER  - 

@article{
author = "Lukić, Jovanka and Strahinić, Ivana and Jovčić, Branko and Filipić, Brankica and Topisirović, Ljubiša and Kojić, Milan and Begović, Jelena",
year = "2012",
abstract = "Adhesion of bacteria to mucosal surfaces and epithelial cells is one of the key features for the selection of probiotics. In this study, we assessed the adhesion property of Lactococcus lactis subsp. lactis BGKP1 based on its strong autoaggregation phenotype and the presence of the mucin binding protein (MbpL). Genes involved in aggregation (aggL) and possible interaction with mucin (mbpL), present on the same plasmid pKP1, were previously separately cloned in the plasmid pAZIL. In vivo and in vitro experiments revealed potentially different physiological roles of these two proteins in the process of adherence to the intestine during the passage of the strain through the gastrointestinal tract. We correlated the in vitro and in vivo aggregation of the BGKP1-20 carrying plasmid with aggL to binding to the colonic mucus through nonspecific hydrophobic interactions. The expression of AggL on the bacterial cell surface significantly increased the hydrophobicity of the strain. On the other hand, the presence of AggL in the strain reduced its ability to adhere to the ileum. Moreover, MbpL protein showed an affinity to bind gastric type mucin proteins such as MUC5AC. This protein did not contribute to the binding of the strain to the ileal or colonic part of the intestine. Different potential functions of lactococcal AggL and MbpL proteins in the process of adhesion to the gastrointestinal tract are proposed.",
publisher = "Amer Soc Microbiology, Washington",
journal = "Applied and Environmental Microbiology",
title = "Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa",
volume = "78",
number = "22",
pages = "7993-8000",
doi = "10.1128/AEM.02141-12"
}

Lukić, J., Strahinić, I., Jovčić, B., Filipić, B., Topisirović, L., Kojić, M.,& Begović, J.. (2012). Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa. in Applied and Environmental Microbiology
Amer Soc Microbiology, Washington., 78(22), 7993-8000.
https://doi.org/10.1128/AEM.02141-12

Lukić J, Strahinić I, Jovčić B, Filipić B, Topisirović L, Kojić M, Begović J. Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa. in Applied and Environmental Microbiology. 2012;78(22):7993-8000.
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Lukić, Jovanka, Strahinić, Ivana, Jovčić, Branko, Filipić, Brankica, Topisirović, Ljubiša, Kojić, Milan, Begović, Jelena, "Different Roles for Lactococcal Aggregation Factor and Mucin Binding Protein in Adhesion to Gastrointestinal Mucosa" in Applied and Environmental Microbiology, 78, no. 22 (2012):7993-8000,
https://doi.org/10.1128/AEM.02141-12 . .