TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Milutinović, Violeta
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5417
AB  - Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.
PB  - MDPI
T2  - Antibiotics
T1  - Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)
VL  - 13
IS  - 1
SP  - 41
DO  - 10.3390/antibiotics13010041
ER  - 

@article{
author = "Ušjak, Ljuboš and Stojković, Dejan and Carević, Tamara and Milutinović, Violeta and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)",
volume = "13",
number = "1",
pages = "41",
doi = "10.3390/antibiotics13010041"
}

Ušjak, L., Stojković, D., Carević, T., Milutinović, V., Soković, M., Niketić, M.,& Petrović, S.. (2024). Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics
MDPI., 13(1), 41.
https://doi.org/10.3390/antibiotics13010041

Ušjak L, Stojković D, Carević T, Milutinović V, Soković M, Niketić M, Petrović S. Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics. 2024;13(1):41.
doi:10.3390/antibiotics13010041 .

Ušjak, Ljuboš, Stojković, Dejan, Carević, Tamara, Milutinović, Violeta, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)" in Antibiotics, 13, no. 1 (2024):41,
https://doi.org/10.3390/antibiotics13010041 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5418
AB  - The aim of this work was to investigate the composition of essential oils isolated from fruits of Peucedanum longifolium, and Rhizomatophora aegopodioides (a species which was previously placed in the genus Peucedanum), as well as to compare the obtained results to those available for other previously investigated related species (including taxa which are also, according to some authors, excluded from the genus Peucedanum). Essential oils were obtained via hydrodistillation in a Clevenger-type apparatus and their composition was analyzed using GC-FID and GC-MS. To compare these data to those of previously investigated taxa, a chemometric approach was applied; the data were analyzed using multivariate statistical methods: non-metric multidimensional scaling (nMDS) and hierarchical cluster analysis. The most abundant in P. longifolium essential oil were monoterpenes (79.7%), mostly α-phellandrene (26.2%), β-phellandrene + limonene (21.0%) and myrcene (9.5%), followed by sesquiterpenes (18.3%), mostly germacrene B (9.5%). On the other hand, dominant in R. aegopodioides essential oil were non-terpenic aliphatic hydrocarbons (46.1%), mainly n-undecane (16.5%) and n-nonane (11.3%). In addition, this essential oil also contained a notable quantity of sesquiterpenes (25.1%), with (E)-sesquilavandulol being the most abundant (10.0%). The results of multivariate statistics revealed a clear separation of the essential oil composition of R. aegopodioides and P. longifolium, as well as of P. longifolium and P. officinale. The clustering of the samples of most of the taxa that do not belong to the Peucedanum in the narrow sense (sensu stricto) was also observed, which is in accordance with their recent inclusion in separate genera.
PB  - MDPI
T2  - Separations
T1  - Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach
VL  - 11
IS  - 1
SP  - 14
DO  - 10.3390/separations11010014
ER  - 

@article{
author = "Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "The aim of this work was to investigate the composition of essential oils isolated from fruits of Peucedanum longifolium, and Rhizomatophora aegopodioides (a species which was previously placed in the genus Peucedanum), as well as to compare the obtained results to those available for other previously investigated related species (including taxa which are also, according to some authors, excluded from the genus Peucedanum). Essential oils were obtained via hydrodistillation in a Clevenger-type apparatus and their composition was analyzed using GC-FID and GC-MS. To compare these data to those of previously investigated taxa, a chemometric approach was applied; the data were analyzed using multivariate statistical methods: non-metric multidimensional scaling (nMDS) and hierarchical cluster analysis. The most abundant in P. longifolium essential oil were monoterpenes (79.7%), mostly α-phellandrene (26.2%), β-phellandrene + limonene (21.0%) and myrcene (9.5%), followed by sesquiterpenes (18.3%), mostly germacrene B (9.5%). On the other hand, dominant in R. aegopodioides essential oil were non-terpenic aliphatic hydrocarbons (46.1%), mainly n-undecane (16.5%) and n-nonane (11.3%). In addition, this essential oil also contained a notable quantity of sesquiterpenes (25.1%), with (E)-sesquilavandulol being the most abundant (10.0%). The results of multivariate statistics revealed a clear separation of the essential oil composition of R. aegopodioides and P. longifolium, as well as of P. longifolium and P. officinale. The clustering of the samples of most of the taxa that do not belong to the Peucedanum in the narrow sense (sensu stricto) was also observed, which is in accordance with their recent inclusion in separate genera.",
publisher = "MDPI",
journal = "Separations",
title = "Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach",
volume = "11",
number = "1",
pages = "14",
doi = "10.3390/separations11010014"
}

Ušjak, L., Niketić, M.,& Petrović, S.. (2024). Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach. in Separations
MDPI., 11(1), 14.
https://doi.org/10.3390/separations11010014

Ušjak L, Niketić M, Petrović S. Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach. in Separations. 2024;11(1):14.
doi:10.3390/separations11010014 .

Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach" in Separations, 11, no. 1 (2024):14,
https://doi.org/10.3390/separations11010014 . .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5267
AB  - Биљке рода Micromeria Benth. (Lamiaceae) традиционално се користе код срчаних
тегоба, респираторних болести (астме), главобоље, прехладе, у третману рана и инфекција
коже. Неке од њих су испитиване у погледу композиције секундарних метаболита, као и
фармаколошких активности. ...
AB  - Species belonging to the genus Micromeria Benth. (Lamiaceae) are traditionally used in the
treatment of heart problems, respiratory diseases (asthma), headaches, cold, wounds and skin
infections. For some of them the composition of secondary metabolites, as well as pharmacological
activities were investigated. ...
PB  - Ботаничко друштво „Андреас Волни”, Нови Сад
PB  - Матица српска, Нови Сад
C3  - 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
T1  - Хемијски састав етарског уља и воденоетанолног екстракта хербе Micromeria nervosa (Desf.) Benth. (Lamiaceae)
T1  - Chemical composition of essential oil and hydroethanol extract of herb of Micromeria nervosa (Desf.) Benth. (Lamiaceae)
SP  - 21
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5267
ER  - 

@conference{
author = "Milutinović, Violeta and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Биљке рода Micromeria Benth. (Lamiaceae) традиционално се користе код срчаних
тегоба, респираторних болести (астме), главобоље, прехладе, у третману рана и инфекција
коже. Неке од њих су испитиване у погледу композиције секундарних метаболита, као и
фармаколошких активности. ..., Species belonging to the genus Micromeria Benth. (Lamiaceae) are traditionally used in the
treatment of heart problems, respiratory diseases (asthma), headaches, cold, wounds and skin
infections. For some of them the composition of secondary metabolites, as well as pharmacological
activities were investigated. ...",
publisher = "Ботаничко друштво „Андреас Волни”, Нови Сад, Матица српска, Нови Сад",
journal = "2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака",
title = "Хемијски састав етарског уља и воденоетанолног екстракта хербе Micromeria nervosa (Desf.) Benth. (Lamiaceae), Chemical composition of essential oil and hydroethanol extract of herb of Micromeria nervosa (Desf.) Benth. (Lamiaceae)",
pages = "21-22",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5267"
}

Milutinović, V., Ušjak, L., Niketić, M.,& Petrović, S.. (2023). Хемијски састав етарског уља и воденоетанолног екстракта хербе Micromeria nervosa (Desf.) Benth. (Lamiaceae). in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака
Ботаничко друштво „Андреас Волни”, Нови Сад., 21-22.
https://hdl.handle.net/21.15107/rcub_farfar_5267

Milutinović V, Ušjak L, Niketić M, Petrović S. Хемијски састав етарског уља и воденоетанолног екстракта хербе Micromeria nervosa (Desf.) Benth. (Lamiaceae). in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака. 2023;:21-22.
https://hdl.handle.net/21.15107/rcub_farfar_5267 .

Milutinović, Violeta, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Хемијски састав етарског уља и воденоетанолног екстракта хербе Micromeria nervosa (Desf.) Benth. (Lamiaceae)" in 2. Симпозијум "Трећи век ботанике у Војводини", Књига сажетака (2023):21-22,
https://hdl.handle.net/21.15107/rcub_farfar_5267 .

TY  - JOUR
AU  - Milutinović, Violeta
AU  - Petrović, Predrag
AU  - Petković, Miloš
AU  - Klaus, Anita
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5180
AB  - The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.), were investigated. Furthermore, the anticholinesterase activity of 12 selected secondary metabolites of these extracts was evaluated. Using semi-preparative LC-MS, five caffeoylquinic acids and the sesquiterpene lactone crepiside E were isolated from H. scheppigianum underground parts extract. All these compounds were also identified in the underground parts extract of H. naegelianum. Quantitative LC-MS analysis showed that the analysed underground parts extracts were rich in both caffeoylquinic acids (139.77 and 156.62 mg/g of extract, respectively) and crepiside E (126.88 and 116.58 mg/g). In the Ellman method, the tested extracts showed an interesting anti-AChE and/or anti-BChE activity (IC50=0.56–1.58 mg/mL), which can be explained, at least partially, by the presence of some of their constituents. Among the metabolites tested, the best activity was revealed for the flavonoids apigenin, luteolin and diosmetin, and the sesquiterpene lactone 8-epiixerisamine A (IC50=68.09–299.37 μM).
PB  - John Wiley and Sons Inc
T2  - Chemistry and Biodiversity
T1  - Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites
VL  - 20
IS  - 11
SP  - e202301044
DO  - 10.1002/cbdv.202301044
ER  - 

@article{
author = "Milutinović, Violeta and Petrović, Predrag and Petković, Miloš and Klaus, Anita and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.), were investigated. Furthermore, the anticholinesterase activity of 12 selected secondary metabolites of these extracts was evaluated. Using semi-preparative LC-MS, five caffeoylquinic acids and the sesquiterpene lactone crepiside E were isolated from H. scheppigianum underground parts extract. All these compounds were also identified in the underground parts extract of H. naegelianum. Quantitative LC-MS analysis showed that the analysed underground parts extracts were rich in both caffeoylquinic acids (139.77 and 156.62 mg/g of extract, respectively) and crepiside E (126.88 and 116.58 mg/g). In the Ellman method, the tested extracts showed an interesting anti-AChE and/or anti-BChE activity (IC50=0.56–1.58 mg/mL), which can be explained, at least partially, by the presence of some of their constituents. Among the metabolites tested, the best activity was revealed for the flavonoids apigenin, luteolin and diosmetin, and the sesquiterpene lactone 8-epiixerisamine A (IC50=68.09–299.37 μM).",
publisher = "John Wiley and Sons Inc",
journal = "Chemistry and Biodiversity",
title = "Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites",
volume = "20",
number = "11",
pages = "e202301044",
doi = "10.1002/cbdv.202301044"
}

Milutinović, V., Petrović, P., Petković, M., Klaus, A., Ušjak, L., Niketić, M.,& Petrović, S.. (2023). Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites. in Chemistry and Biodiversity
John Wiley and Sons Inc., 20(11), e202301044.
https://doi.org/10.1002/cbdv.202301044

Milutinović V, Petrović P, Petković M, Klaus A, Ušjak L, Niketić M, Petrović S. Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites. in Chemistry and Biodiversity. 2023;20(11):e202301044.
doi:10.1002/cbdv.202301044 .

Milutinović, Violeta, Petrović, Predrag, Petković, Miloš, Klaus, Anita, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites" in Chemistry and Biodiversity, 20, no. 11 (2023):e202301044,
https://doi.org/10.1002/cbdv.202301044 . .

TY  - CONF
AU  - Kukić-Marković, Jelena
AU  - Milutinović, Violeta
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5086
AB  - Stachys subcrenata Vis. (Lamiaceae) је вишегодишња зељаста биљка која припада комплексној групи S. recta и данас се углавном сматра њеном подврстом. Описана је из Далмације, и до сада је са сигурношћу позната за приобаље и залеђе Јадранског мора, иако се наводи и за источни и јужни Балкан, Анадолију и Иран. ...
AB  - Stachys subcrenata Vis. (Lamiaceae) is a perennial herb that belongs to the complex group S. recta and nowadays it is mostly considered аs its subspecies. It has been described from Dalmatia, and so far it is known with certainty for the coast and hinterland of the Adriatic Sea, although it was also reported for the eastern and southern Balkans, Anatolia and Iran. Plant inhabits stony and rocky places, often by the sea coast. ...
PB  - Ботаничко друштво „Андреас Волни”, Нови Сад
PB  - Матица српска, Нови Сад
C3  - 2. Симпозијум  "Трећи век ботанике у Војводини", Књига сажетака
T1  - Фенолни састојци метанолног екстракта хербе Stachys subcrenata
T1  - Phenolic constituents of Stachys subcrenata herb methanol extract
SP  - 24
EP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5086
ER  - 

@conference{
author = "Kukić-Marković, Jelena and Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Stachys subcrenata Vis. (Lamiaceae) је вишегодишња зељаста биљка која припада комплексној групи S. recta и данас се углавном сматра њеном подврстом. Описана је из Далмације, и до сада је са сигурношћу позната за приобаље и залеђе Јадранског мора, иако се наводи и за источни и јужни Балкан, Анадолију и Иран. ..., Stachys subcrenata Vis. (Lamiaceae) is a perennial herb that belongs to the complex group S. recta and nowadays it is mostly considered аs its subspecies. It has been described from Dalmatia, and so far it is known with certainty for the coast and hinterland of the Adriatic Sea, although it was also reported for the eastern and southern Balkans, Anatolia and Iran. Plant inhabits stony and rocky places, often by the sea coast. ...",
publisher = "Ботаничко друштво „Андреас Волни”, Нови Сад, Матица српска, Нови Сад",
journal = "2. Симпозијум  "Трећи век ботанике у Војводини", Књига сажетака",
title = "Фенолни састојци метанолног екстракта хербе Stachys subcrenata, Phenolic constituents of Stachys subcrenata herb methanol extract",
pages = "24-26",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5086"
}

Kukić-Marković, J., Milutinović, V., Niketić, M.,& Petrović, S.. (2023). Фенолни састојци метанолног екстракта хербе Stachys subcrenata. in 2. Симпозијум  "Трећи век ботанике у Војводини", Књига сажетака
Ботаничко друштво „Андреас Волни”, Нови Сад., 24-26.
https://hdl.handle.net/21.15107/rcub_farfar_5086

Kukić-Marković J, Milutinović V, Niketić M, Petrović S. Фенолни састојци метанолног екстракта хербе Stachys subcrenata. in 2. Симпозијум  "Трећи век ботанике у Војводини", Књига сажетака. 2023;:24-26.
https://hdl.handle.net/21.15107/rcub_farfar_5086 .

Kukić-Marković, Jelena, Milutinović, Violeta, Niketić, Marjan, Petrović, Silvana, "Фенолни састојци метанолног екстракта хербе Stachys subcrenata" in 2. Симпозијум  "Трећи век ботанике у Војводини", Књига сажетака (2023):24-26,
https://hdl.handle.net/21.15107/rcub_farfar_5086 .

TY  - CONF
AU  - Kukić-Marković, Jelena
AU  - Milutinović, Violeta
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4988
AB  - The highest richness of taxa within genus Stachys was recorded in the wider area of the Mediterranean, where the group S. recta stands out in terms of taxonomic complexity. The center of diversity of this group is on the Balkan Peninsula. Its representatives are still insufficiently defined in taxonomic and horological terms, and their traditional approach includes about fifteen taxa, mostly at the infraspecific level. Despite some radical approaches that were presented at the end of the last century [1], the traditional taxonomic treatment is still prevailed in the relevant global lists.
In this work antiradical potential and phenolic constituents of S. recta L. subsp. recta were investigated. Plant material, collected in Central Serbia (vicinity of Prokuplje), was air-dried, powdered, and then extracted by bimaceration with chloroform and methanol. Obtained dry methanol extract exhibited strong, concentration-dependant anti-DPPH activity (SC50=8.23 μg/mL), with total phenolics content at 0.27 mg GAE/mg of dry extract. In TLC-DPPH test observed yellow zones indicated presence of few compounds which strongly neutralized purple DPPH radical. LC-MS analysis revealed presence of 24 phenolic compounds, belonging to three classes of constituents: phenolic acids, phenylethanol glycosides and flavonoids. Chlorogenic acid (2.63%) was the only phenolic acid, while acteoside was the most dominant phenylethanoid (24.03%). Flavonoid fraction was composed almost entirely of 8-hydroxyflavones, derivatives of hypolaetine and isoscutellareine (53.47%). The most prominent flavonoids in investigated extract were 7-O-[6'''-acetylallosyl(1®2)]-glucopyranosides of 4'-O-methylhypolaetine and hypolaetine (15.92 and 13.01%, respectively).
C3  - 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts
T1  - Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract
SP  - 196
EP  - 196
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4988
ER  - 

@conference{
author = "Kukić-Marković, Jelena and Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "The highest richness of taxa within genus Stachys was recorded in the wider area of the Mediterranean, where the group S. recta stands out in terms of taxonomic complexity. The center of diversity of this group is on the Balkan Peninsula. Its representatives are still insufficiently defined in taxonomic and horological terms, and their traditional approach includes about fifteen taxa, mostly at the infraspecific level. Despite some radical approaches that were presented at the end of the last century [1], the traditional taxonomic treatment is still prevailed in the relevant global lists.
In this work antiradical potential and phenolic constituents of S. recta L. subsp. recta were investigated. Plant material, collected in Central Serbia (vicinity of Prokuplje), was air-dried, powdered, and then extracted by bimaceration with chloroform and methanol. Obtained dry methanol extract exhibited strong, concentration-dependant anti-DPPH activity (SC50=8.23 μg/mL), with total phenolics content at 0.27 mg GAE/mg of dry extract. In TLC-DPPH test observed yellow zones indicated presence of few compounds which strongly neutralized purple DPPH radical. LC-MS analysis revealed presence of 24 phenolic compounds, belonging to three classes of constituents: phenolic acids, phenylethanol glycosides and flavonoids. Chlorogenic acid (2.63%) was the only phenolic acid, while acteoside was the most dominant phenylethanoid (24.03%). Flavonoid fraction was composed almost entirely of 8-hydroxyflavones, derivatives of hypolaetine and isoscutellareine (53.47%). The most prominent flavonoids in investigated extract were 7-O-[6'''-acetylallosyl(1®2)]-glucopyranosides of 4'-O-methylhypolaetine and hypolaetine (15.92 and 13.01%, respectively).",
journal = "5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts",
title = "Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract",
pages = "196-196",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4988"
}

Kukić-Marković, J., Milutinović, V., Niketić, M.,& Petrović, S.. (2023). Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract. in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts, 196-196.
https://hdl.handle.net/21.15107/rcub_farfar_4988

Kukić-Marković J, Milutinović V, Niketić M, Petrović S. Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract. in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts. 2023;:196-196.
https://hdl.handle.net/21.15107/rcub_farfar_4988 .

Kukić-Marković, Jelena, Milutinović, Violeta, Niketić, Marjan, Petrović, Silvana, "Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract" in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts (2023):196-196,
https://hdl.handle.net/21.15107/rcub_farfar_4988 .

TY  - CONF
AU  - Jovanović, Aleksandar
AU  - Stefanović-Vojinović, Jelena
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Tadić, Ivana
AU  - Catić-Đorđević, Aleksandra
AU  - Kukić-Marković, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5042
AB  - Background: Although the use of medicinal plants have been known since ancient times, an increased use of herbal products is evident in the last few decades, mainly as a part of self-care treatments. In addition to the positive health effects, the use of herbal products can cause certain side effects as well as interactions with conventional therapy, especially among elderly patients.
Purpose: To examine the awareness, habits and attitudes of elderly patients towards the use of herbal products in the Republic of Serbia.
Method: The quantitative, non-experimental research was conducted using a questionnaire in public pharmacies during February 2020. Patients older than 60 years were included in the study. The study was anonymous, and patients gave informed consent.
Results: The study involved 436 patients, of whom 62.4% were female. Most of the respondents live in the city (72.9%) and have a high school or lower education degree (61.9%). Most patients (32.3%) use herbal products daily, while only 12.1% rarely or never use them. Every fourth patient (24.5%) uses them for treatment, while 69.5% to improve their health. Although the majority of patients purchase herbal products in pharmacies and in health food stores (53.6% and 20.8%, respectively), a significant number (19.3%) collect medicinal plants and prepare herbal preparations themselves. The main sources of information about medicinal plants / herbal products are pharmacists (39.7%) and people from the immediate area (28.4%), followed by the media (18.2%) and physicians (11.1%). Every fourth patient (24.5%) uses herbal products for the treatment of some health issues, while 69.5% of patients use herbal products to improve their health. 48.9% of them believe that herbal products are safe, but with some side effects, while 35.8% believe herbal products are completely free of any side effects. Half of respondents (51.4%) believe that herbal products are as effective as or more effective than synthetic medicines. Less than one-third of patients (29.8%) indicated that they knew the difference between herbal medicinal products and herbal dietary supplements. Still, a third of patients (36.7%) needed additional explanations for the use of herbal products. 76.6% of patients stated that they always receive advice from a pharmacist when purchasing herbal products.
Conclusion: The survey conducted in this work showed that elderly patients use herbal products to a considerable extent, which means that it is necessary to educate elderly patients on this matter. Pharmacists should play a vital role in this process and actively discuss the characteristics and proper use of herbal products with patients, especially having in mind the high possibility of interactions with conventional medicines and occurrence of side effects. Since the quality of some herbal products sometimes could be questionable, especially non-regulated ones, patients should be encouraged to purchase them from pharmacies and consult with pharmacists.
PB  - The International Pharmaceutical Federation (FIP)
C3  - Pharmacy Education
T1  - Awareness of elderly patients on herbal products in Serbia
VL  - 23
IS  - 5
SP  - 29
EP  - 29
DO  - 10.46542/pe.2023.235.138
ER  - 

@conference{
author = "Jovanović, Aleksandar and Stefanović-Vojinović, Jelena and Petrović, Silvana and Drobac, Milica and Tadić, Ivana and Catić-Đorđević, Aleksandra and Kukić-Marković, Jelena",
year = "2023",
abstract = "Background: Although the use of medicinal plants have been known since ancient times, an increased use of herbal products is evident in the last few decades, mainly as a part of self-care treatments. In addition to the positive health effects, the use of herbal products can cause certain side effects as well as interactions with conventional therapy, especially among elderly patients.
Purpose: To examine the awareness, habits and attitudes of elderly patients towards the use of herbal products in the Republic of Serbia.
Method: The quantitative, non-experimental research was conducted using a questionnaire in public pharmacies during February 2020. Patients older than 60 years were included in the study. The study was anonymous, and patients gave informed consent.
Results: The study involved 436 patients, of whom 62.4% were female. Most of the respondents live in the city (72.9%) and have a high school or lower education degree (61.9%). Most patients (32.3%) use herbal products daily, while only 12.1% rarely or never use them. Every fourth patient (24.5%) uses them for treatment, while 69.5% to improve their health. Although the majority of patients purchase herbal products in pharmacies and in health food stores (53.6% and 20.8%, respectively), a significant number (19.3%) collect medicinal plants and prepare herbal preparations themselves. The main sources of information about medicinal plants / herbal products are pharmacists (39.7%) and people from the immediate area (28.4%), followed by the media (18.2%) and physicians (11.1%). Every fourth patient (24.5%) uses herbal products for the treatment of some health issues, while 69.5% of patients use herbal products to improve their health. 48.9% of them believe that herbal products are safe, but with some side effects, while 35.8% believe herbal products are completely free of any side effects. Half of respondents (51.4%) believe that herbal products are as effective as or more effective than synthetic medicines. Less than one-third of patients (29.8%) indicated that they knew the difference between herbal medicinal products and herbal dietary supplements. Still, a third of patients (36.7%) needed additional explanations for the use of herbal products. 76.6% of patients stated that they always receive advice from a pharmacist when purchasing herbal products.
Conclusion: The survey conducted in this work showed that elderly patients use herbal products to a considerable extent, which means that it is necessary to educate elderly patients on this matter. Pharmacists should play a vital role in this process and actively discuss the characteristics and proper use of herbal products with patients, especially having in mind the high possibility of interactions with conventional medicines and occurrence of side effects. Since the quality of some herbal products sometimes could be questionable, especially non-regulated ones, patients should be encouraged to purchase them from pharmacies and consult with pharmacists.",
publisher = "The International Pharmaceutical Federation (FIP)",
journal = "Pharmacy Education",
title = "Awareness of elderly patients on herbal products in Serbia",
volume = "23",
number = "5",
pages = "29-29",
doi = "10.46542/pe.2023.235.138"
}

Jovanović, A., Stefanović-Vojinović, J., Petrović, S., Drobac, M., Tadić, I., Catić-Đorđević, A.,& Kukić-Marković, J.. (2023). Awareness of elderly patients on herbal products in Serbia. in Pharmacy Education
The International Pharmaceutical Federation (FIP)., 23(5), 29-29.
https://doi.org/10.46542/pe.2023.235.138

Jovanović A, Stefanović-Vojinović J, Petrović S, Drobac M, Tadić I, Catić-Đorđević A, Kukić-Marković J. Awareness of elderly patients on herbal products in Serbia. in Pharmacy Education. 2023;23(5):29-29.
doi:10.46542/pe.2023.235.138 .

Jovanović, Aleksandar, Stefanović-Vojinović, Jelena, Petrović, Silvana, Drobac, Milica, Tadić, Ivana, Catić-Đorđević, Aleksandra, Kukić-Marković, Jelena, "Awareness of elderly patients on herbal products in Serbia" in Pharmacy Education, 23, no. 5 (2023):29-29,
https://doi.org/10.46542/pe.2023.235.138 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Milenković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4469
AB  - Composition and antimicrobial activity of root, leaf, stem, flower and fruit essential oils from
cultivated Pastinaca sativa subsp. sativa, and its two wild-growing relatives P. sativa subsp. urens
and P. hirsuta (Apiaceae) were investigated. Twenty-nine hydrodistilled essential oils of plants from
different localities and/or years were analysed by GC-FID and GC/MS. Dominant in root oils was
myristicin (P. sativa) or apiole (P. hirsuta), in leaf and stem oils myristicin (cultivated plants) or γ-palmitolactone (wild-growing plants) and in flower and fruit oils aliphatic esters. Multivariate
statistics (PCA, nMDS, UPGMA clustering) generally revealed separation of oils of investigated
Pastinaca taxa and demonstrated their chemosystematic significance. One oil per each organ of all three plants (fifteen in total) was tested using microdilution method for activity against Candida
tropicalis, C. parapsilosis, C. krusei, C. glabrata, C. albicans, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium and Enterobacter cloacae; MIC = 0.25–8 mg/mL, MBC(MFC) = 0.5–16 mg/mL.
PB  - Taylor and Francis Group
T2  - Journal of Essential Oil Research
T1  - Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils
VL  - 35
IS  - 2
SP  - 154
EP  - 167
DO  - 10.1080/10412905.2022.2147675
ER  - 

@article{
author = "Ušjak, Ljuboš and Drobac, Milica and Ivanov, Marija and Soković, Marina and Milenković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Composition and antimicrobial activity of root, leaf, stem, flower and fruit essential oils from
cultivated Pastinaca sativa subsp. sativa, and its two wild-growing relatives P. sativa subsp. urens
and P. hirsuta (Apiaceae) were investigated. Twenty-nine hydrodistilled essential oils of plants from
different localities and/or years were analysed by GC-FID and GC/MS. Dominant in root oils was
myristicin (P. sativa) or apiole (P. hirsuta), in leaf and stem oils myristicin (cultivated plants) or γ-palmitolactone (wild-growing plants) and in flower and fruit oils aliphatic esters. Multivariate
statistics (PCA, nMDS, UPGMA clustering) generally revealed separation of oils of investigated
Pastinaca taxa and demonstrated their chemosystematic significance. One oil per each organ of all three plants (fifteen in total) was tested using microdilution method for activity against Candida
tropicalis, C. parapsilosis, C. krusei, C. glabrata, C. albicans, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium and Enterobacter cloacae; MIC = 0.25–8 mg/mL, MBC(MFC) = 0.5–16 mg/mL.",
publisher = "Taylor and Francis Group",
journal = "Journal of Essential Oil Research",
title = "Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils",
volume = "35",
number = "2",
pages = "154-167",
doi = "10.1080/10412905.2022.2147675"
}

Ušjak, L., Drobac, M., Ivanov, M., Soković, M., Milenković, M., Niketić, M.,& Petrović, S.. (2023). Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils. in Journal of Essential Oil Research
Taylor and Francis Group., 35(2), 154-167.
https://doi.org/10.1080/10412905.2022.2147675

Ušjak L, Drobac M, Ivanov M, Soković M, Milenković M, Niketić M, Petrović S. Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils. in Journal of Essential Oil Research. 2023;35(2):154-167.
doi:10.1080/10412905.2022.2147675 .

Ušjak, Ljuboš, Drobac, Milica, Ivanov, Marija, Soković, Marina, Milenković, Marina, Niketić, Marjan, Petrović, Silvana, "Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils" in Journal of Essential Oil Research, 35, no. 2 (2023):154-167,
https://doi.org/10.1080/10412905.2022.2147675 . .

TY  - CONF
AU  - Kukić-Marković, Jelena
AU  - Petković, Miloš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4987
AB  - Stachys annua (L.) L. (annual yellow-woundwort) is an arrable weed and melliferous archaeophyte, with the native range in Europe to Siberia and Iran [1]. The aim of this work is to implement simple chromatographic technique for isolation of flavonoids from crude methanol extract of S. annua aerial flowering parts (herb).  
Plant material collected in NW Serbia (Mt Sokolske planine) was air-dried, powdered, and then extracted by bimaceration with chloroform and methanol. Dry methanol extract of S. annua herb was applied on preparative TLC plates of silica gel. After developing of chromatogram in methylene chloride:methanol:water (40:10:1, V/V/V), and spraying with NP/PEG reagent, in ultraviolet light at 365 nm three clearly separated fluorescence-quenching zones were observed. Zones were eluted with mixture of methylene chloride and methanol (1:1, V/V), and using LC-MS analysis one dominant compound was detected in each of these fractions. According to their UV, MS and 1H-NMR spectral data, as well as using flavonoids previously isolated from S. alpina subsp. dinarica [2], these compounds (1-3) were identified as 8-hydroxyflavone glycosides, namely: isoscutellarein-7-O-[6'''-acetyl-β-D-allopyranosyl-(1→2)]-β-D-glucopyranoside (compound 1), 3'-hydroxy-4'-O-methylisoscutellareine-7-O-[6'''-acetyl-β-D-allopyranosyl-(1→2)]-β-D-glucopyranoside (compound 2) and 4'-O-methylisoscutellareine-7-O-[6'''-acetyl-β-D-allopyranosyl-(1→2)]-β-D-glucopyranoside (compound 3).
It could be concluded that the developed preparative TLC method is suitable for rapid isolation of the mentioned flavonoid biosides from the crude methanol extract of S. annua without prior fractionation by other chromatographic techniques.
C3  - 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts
T1  - Structural elucidation of flavonoids isolated from aerial parts of Stachys annua (L.) L. (Lamiaceae)
SP  - 194
EP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4987
ER  - 

@conference{
author = "Kukić-Marković, Jelena and Petković, Miloš and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Stachys annua (L.) L. (annual yellow-woundwort) is an arrable weed and melliferous archaeophyte, with the native range in Europe to Siberia and Iran [1]. The aim of this work is to implement simple chromatographic technique for isolation of flavonoids from crude methanol extract of S. annua aerial flowering parts (herb).  
Plant material collected in NW Serbia (Mt Sokolske planine) was air-dried, powdered, and then extracted by bimaceration with chloroform and methanol. Dry methanol extract of S. annua herb was applied on preparative TLC plates of silica gel. After developing of chromatogram in methylene chloride:methanol:water (40:10:1, V/V/V), and spraying with NP/PEG reagent, in ultraviolet light at 365 nm three clearly separated fluorescence-quenching zones were observed. Zones were eluted with mixture of methylene chloride and methanol (1:1, V/V), and using LC-MS analysis one dominant compound was detected in each of these fractions. According to their UV, MS and 1H-NMR spectral data, as well as using flavonoids previously isolated from S. alpina subsp. dinarica [2], these compounds (1-3) were identified as 8-hydroxyflavone glycosides, namely: isoscutellarein-7-O-[6'''-acetyl-β-D-allopyranosyl-(1→2)]-β-D-glucopyranoside (compound 1), 3'-hydroxy-4'-O-methylisoscutellareine-7-O-[6'''-acetyl-β-D-allopyranosyl-(1→2)]-β-D-glucopyranoside (compound 2) and 4'-O-methylisoscutellareine-7-O-[6'''-acetyl-β-D-allopyranosyl-(1→2)]-β-D-glucopyranoside (compound 3).
It could be concluded that the developed preparative TLC method is suitable for rapid isolation of the mentioned flavonoid biosides from the crude methanol extract of S. annua without prior fractionation by other chromatographic techniques.",
journal = "5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts",
title = "Structural elucidation of flavonoids isolated from aerial parts of Stachys annua (L.) L. (Lamiaceae)",
pages = "194-195",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4987"
}

Kukić-Marković, J., Petković, M., Niketić, M.,& Petrović, S.. (2023). Structural elucidation of flavonoids isolated from aerial parts of Stachys annua (L.) L. (Lamiaceae). in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts, 194-195.
https://hdl.handle.net/21.15107/rcub_farfar_4987

Kukić-Marković J, Petković M, Niketić M, Petrović S. Structural elucidation of flavonoids isolated from aerial parts of Stachys annua (L.) L. (Lamiaceae). in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts. 2023;:194-195.
https://hdl.handle.net/21.15107/rcub_farfar_4987 .

Kukić-Marković, Jelena, Petković, Miloš, Niketić, Marjan, Petrović, Silvana, "Structural elucidation of flavonoids isolated from aerial parts of Stachys annua (L.) L. (Lamiaceae)" in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts (2023):194-195,
https://hdl.handle.net/21.15107/rcub_farfar_4987 .

TY  - CONF
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4901
AB  - Composition of essential oils from ripe dried fruits of Peucedanum longifolium Waldst. & Kit. (PL) and P. aegopodioides (Boiss.) Vandas (PA) was investigated for the first time. Both taxa are distributed on the Balkan Peninsula, Asia Minor and Transcaucasia, and some authors include the last species in a separate genus, Rhizomatophora Pimenov [1]. Plant material was collected in Serbia, PL on Vis hill in Sićevo Gorge, PA in vicinity of Pirot, near village Basara. Oils were obtained by hydrodistillation in Clevenger-type apparatus; yields 0.91% (PL) and 0.02% (PA), w/w. GC-FID-MS analysis revealed presence of 46 components in PL oil and 48 in PA oil, accounting for 98.0% and 90.0% of total oils. PL oil was dominated by monoterpene hydrocarbons (74.4%), mainly α-phellandrene (26.2%), β-phellandrene + limonene (21.0%) and myrcene (9.5%), followed by sesquiterpene hydrocarbons (17.9%), mainly germacrene B (9.5%). On the other hand, most abundant in PA oil were non-terpenic aliphatic hydrocarbons (46.1%), mainly n-undecane (16.5%) and n-nonane (11.3%). However, this oil also contained significant amounts of both non-oxygenated and oxygenated sesquiterpenes (11.4% and 13.6%), with (E)-sesquilavandulol being most prominent (10.0%). Previously, composition of essential oils from some other plant parts of PL was investigated. For example, essential oil from fresh leaves and young stems of this plant was dominated by sesquiterpene β-elemene (24.7%) and monoterpene (E)-β-ocimene (11.7%) [2], which were present in small amounts in dried fruit oil investigated in current study (0.5% and 0.6%).
C3  - 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts
T1  - Chemical composition of essential oils from fruits of Peucedanum longifolium and P. aegopodioides (Apiaceae)
SP  - 268
EP  - 268
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4901
ER  - 

@conference{
author = "Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Composition of essential oils from ripe dried fruits of Peucedanum longifolium Waldst. & Kit. (PL) and P. aegopodioides (Boiss.) Vandas (PA) was investigated for the first time. Both taxa are distributed on the Balkan Peninsula, Asia Minor and Transcaucasia, and some authors include the last species in a separate genus, Rhizomatophora Pimenov [1]. Plant material was collected in Serbia, PL on Vis hill in Sićevo Gorge, PA in vicinity of Pirot, near village Basara. Oils were obtained by hydrodistillation in Clevenger-type apparatus; yields 0.91% (PL) and 0.02% (PA), w/w. GC-FID-MS analysis revealed presence of 46 components in PL oil and 48 in PA oil, accounting for 98.0% and 90.0% of total oils. PL oil was dominated by monoterpene hydrocarbons (74.4%), mainly α-phellandrene (26.2%), β-phellandrene + limonene (21.0%) and myrcene (9.5%), followed by sesquiterpene hydrocarbons (17.9%), mainly germacrene B (9.5%). On the other hand, most abundant in PA oil were non-terpenic aliphatic hydrocarbons (46.1%), mainly n-undecane (16.5%) and n-nonane (11.3%). However, this oil also contained significant amounts of both non-oxygenated and oxygenated sesquiterpenes (11.4% and 13.6%), with (E)-sesquilavandulol being most prominent (10.0%). Previously, composition of essential oils from some other plant parts of PL was investigated. For example, essential oil from fresh leaves and young stems of this plant was dominated by sesquiterpene β-elemene (24.7%) and monoterpene (E)-β-ocimene (11.7%) [2], which were present in small amounts in dried fruit oil investigated in current study (0.5% and 0.6%).",
journal = "5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts",
title = "Chemical composition of essential oils from fruits of Peucedanum longifolium and P. aegopodioides (Apiaceae)",
pages = "268-268",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4901"
}

Ušjak, L., Niketić, M.,& Petrović, S.. (2023). Chemical composition of essential oils from fruits of Peucedanum longifolium and P. aegopodioides (Apiaceae). in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts, 268-268.
https://hdl.handle.net/21.15107/rcub_farfar_4901

Ušjak L, Niketić M, Petrović S. Chemical composition of essential oils from fruits of Peucedanum longifolium and P. aegopodioides (Apiaceae). in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts. 2023;:268-268.
https://hdl.handle.net/21.15107/rcub_farfar_4901 .

Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Chemical composition of essential oils from fruits of Peucedanum longifolium and P. aegopodioides (Apiaceae)" in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts (2023):268-268,
https://hdl.handle.net/21.15107/rcub_farfar_4901 .

TY  - CONF
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4900
AB  - Composition of dichloromethane and methanol extracts from roots and fruits of Prangos trifida (Mill.) Herrnst. & Heyn s.l. (collected in Sićevo Gorge, Serbia in 2020) was investigated. Extracts were obtained by bimaceration, firstly with dichloromethane, then with methanol; solvents were removed under reduced pressure. GC-FID-MS analysis, conducted after saponification and methylation of samples, showed that palmitic, oleic and linoleic (14.2-51.8%) were dominant fatty acids in root dichloromethane extract, and oleic, linoleic and petroselinic in oily supernatant of fruit dichloromethane extract (10.2-49.9%); in both samples 16 fatty acids were identified. Dominant phytosterols, investigated by GC-FID-MS after unsaponifiable fractions silanization, were β-sitosterol (48.2 and 25.1%) and stigmasterol (14.9 and 12.8%). LC-DAD-MS analysis of dichloromethane and methanol extracts revealed presence of 7-11 furanocoumarins, including oxypeucedanin and imperatorin (up to 105.9 and 49.2 mg/g in fruit dichloromethane extract crystalline precipitate), followed by smaller amounts of oxypeucedanin hydrate, isoimperatorin, heraclenol, heraclenin and/or xanthotoxin. All extracts were rich in one 2',3'-dihydrofuranocoumarin derivative. Previously, such derivative, prantschimgin, as well as imperatorin and isoimperatorin were isolated from this plant and their in vitro anti-inflammatory activity was demonstrated [1]. In current investigation, in root methanol extract, chlorogenic (31.6 mg/g), two dicaffeoylquinic (12.8 and 48.1 mg/g), and smaller amounts of one caffeoylquinic and one feruloylquinic acids were also determined. Furthermore, low quantities of chlorogenic and one dicaffeoylquinic acid and two quercetin-type flavonoids were detected in fruit methanol extract, and one methylluteolin-type flavonoid in both methanol extracts.
C3  - 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts
T1  - Fatty acids, phytosterols, furanocoumarins and polyphenols of Prangos trifida s.l. (Apiaceae) roots and fruits
SP  - 269
EP  - 269
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4900
ER  - 

@conference{
author = "Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Composition of dichloromethane and methanol extracts from roots and fruits of Prangos trifida (Mill.) Herrnst. & Heyn s.l. (collected in Sićevo Gorge, Serbia in 2020) was investigated. Extracts were obtained by bimaceration, firstly with dichloromethane, then with methanol; solvents were removed under reduced pressure. GC-FID-MS analysis, conducted after saponification and methylation of samples, showed that palmitic, oleic and linoleic (14.2-51.8%) were dominant fatty acids in root dichloromethane extract, and oleic, linoleic and petroselinic in oily supernatant of fruit dichloromethane extract (10.2-49.9%); in both samples 16 fatty acids were identified. Dominant phytosterols, investigated by GC-FID-MS after unsaponifiable fractions silanization, were β-sitosterol (48.2 and 25.1%) and stigmasterol (14.9 and 12.8%). LC-DAD-MS analysis of dichloromethane and methanol extracts revealed presence of 7-11 furanocoumarins, including oxypeucedanin and imperatorin (up to 105.9 and 49.2 mg/g in fruit dichloromethane extract crystalline precipitate), followed by smaller amounts of oxypeucedanin hydrate, isoimperatorin, heraclenol, heraclenin and/or xanthotoxin. All extracts were rich in one 2',3'-dihydrofuranocoumarin derivative. Previously, such derivative, prantschimgin, as well as imperatorin and isoimperatorin were isolated from this plant and their in vitro anti-inflammatory activity was demonstrated [1]. In current investigation, in root methanol extract, chlorogenic (31.6 mg/g), two dicaffeoylquinic (12.8 and 48.1 mg/g), and smaller amounts of one caffeoylquinic and one feruloylquinic acids were also determined. Furthermore, low quantities of chlorogenic and one dicaffeoylquinic acid and two quercetin-type flavonoids were detected in fruit methanol extract, and one methylluteolin-type flavonoid in both methanol extracts.",
journal = "5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts",
title = "Fatty acids, phytosterols, furanocoumarins and polyphenols of Prangos trifida s.l. (Apiaceae) roots and fruits",
pages = "269-269",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4900"
}

Ušjak, L., Niketić, M.,& Petrović, S.. (2023). Fatty acids, phytosterols, furanocoumarins and polyphenols of Prangos trifida s.l. (Apiaceae) roots and fruits. in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts, 269-269.
https://hdl.handle.net/21.15107/rcub_farfar_4900

Ušjak L, Niketić M, Petrović S. Fatty acids, phytosterols, furanocoumarins and polyphenols of Prangos trifida s.l. (Apiaceae) roots and fruits. in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts. 2023;:269-269.
https://hdl.handle.net/21.15107/rcub_farfar_4900 .

Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Fatty acids, phytosterols, furanocoumarins and polyphenols of Prangos trifida s.l. (Apiaceae) roots and fruits" in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts (2023):269-269,
https://hdl.handle.net/21.15107/rcub_farfar_4900 .

TY  - JOUR
AU  - Niketić, Marjan
AU  - Tomović, Gordana
AU  - Anačkov, Goran
AU  - Đorđević, Vladan
AU  - Đurović, Sanja
AU  - Duraki, Šemija
AU  - Kabaš, Eva
AU  - Lakušić, Dmitar
AU  - Petkovski, Gorana
AU  - Petrović, Silvana
AU  - Ranimirović, Milana
AU  - Slavkovska, Violeta
AU  - Ušjak, Ljuboš
AU  - Zbiljić, Miloš
AU  - Zlatković, Bojan
AU  - Stevanović, Vladimir
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4958
AB  - This paper represents the fourth part of the inventory of the flora of Serbia (Niketić et al. 2018, 2020, 2021), which contains nomenclatural, taxonomic and floristic notes related to taxa from the Magnoliopsida group. At the same time, this contribution is the basis for publication of subsequent volumes of the An annotated checklist of vascular flora of Serbia in order to supplement the data on vascular plants in our country.
AB  - У раду је предложено пет нових номенклатурних комбинација, а поред тога, представљено је 17 таксона (врста, подврста и хибрида) васкуларне флоре који су нови за флору Србије (од тога 12 аутохтоних и пет алохтоних биљак). Потврђени су стари или непоуздани наводи за две аутохтоне биљке у флори Србије. У последњем делу рада дате су информације о деветнаест таксона чије је присуство у Србији или у њеним територијалним јединицама оповргнуто.
PB  - Natural History Museum, Belgrade, Serbia
T2  - Bulletin of the Natural History Museum
T1  - Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV
T1  - Mатеријал за Kритичку листу врста васкуларне флоре Србије. Номенклатурни, таксономски и флористички прилози IV
VL  - 15
SP  - 27
EP  - 96
DO  - 10.5937/bnhmb2215027N
ER  - 

@article{
author = "Niketić, Marjan and Tomović, Gordana and Anačkov, Goran and Đorđević, Vladan and Đurović, Sanja and Duraki, Šemija and Kabaš, Eva and Lakušić, Dmitar and Petkovski, Gorana and Petrović, Silvana and Ranimirović, Milana and Slavkovska, Violeta and Ušjak, Ljuboš and Zbiljić, Miloš and Zlatković, Bojan and Stevanović, Vladimir",
year = "2022",
abstract = "This paper represents the fourth part of the inventory of the flora of Serbia (Niketić et al. 2018, 2020, 2021), which contains nomenclatural, taxonomic and floristic notes related to taxa from the Magnoliopsida group. At the same time, this contribution is the basis for publication of subsequent volumes of the An annotated checklist of vascular flora of Serbia in order to supplement the data on vascular plants in our country., У раду је предложено пет нових номенклатурних комбинација, а поред тога, представљено је 17 таксона (врста, подврста и хибрида) васкуларне флоре који су нови за флору Србије (од тога 12 аутохтоних и пет алохтоних биљак). Потврђени су стари или непоуздани наводи за две аутохтоне биљке у флори Србије. У последњем делу рада дате су информације о деветнаест таксона чије је присуство у Србији или у њеним територијалним јединицама оповргнуто.",
publisher = "Natural History Museum, Belgrade, Serbia",
journal = "Bulletin of the Natural History Museum",
title = "Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV, Mатеријал за Kритичку листу врста васкуларне флоре Србије. Номенклатурни, таксономски и флористички прилози IV",
volume = "15",
pages = "27-96",
doi = "10.5937/bnhmb2215027N"
}

Niketić, M., Tomović, G., Anačkov, G., Đorđević, V., Đurović, S., Duraki, Š., Kabaš, E., Lakušić, D., Petkovski, G., Petrović, S., Ranimirović, M., Slavkovska, V., Ušjak, L., Zbiljić, M., Zlatković, B.,& Stevanović, V.. (2022). Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV. in Bulletin of the Natural History Museum
Natural History Museum, Belgrade, Serbia., 15, 27-96.
https://doi.org/10.5937/bnhmb2215027N

Niketić M, Tomović G, Anačkov G, Đorđević V, Đurović S, Duraki Š, Kabaš E, Lakušić D, Petkovski G, Petrović S, Ranimirović M, Slavkovska V, Ušjak L, Zbiljić M, Zlatković B, Stevanović V. Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV. in Bulletin of the Natural History Museum. 2022;15:27-96.
doi:10.5937/bnhmb2215027N .

Niketić, Marjan, Tomović, Gordana, Anačkov, Goran, Đorđević, Vladan, Đurović, Sanja, Duraki, Šemija, Kabaš, Eva, Lakušić, Dmitar, Petkovski, Gorana, Petrović, Silvana, Ranimirović, Milana, Slavkovska, Violeta, Ušjak, Ljuboš, Zbiljić, Miloš, Zlatković, Bojan, Stevanović, Vladimir, "Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV" in Bulletin of the Natural History Museum, 15 (2022):27-96,
https://doi.org/10.5937/bnhmb2215027N . .

TY  - JOUR
AU  - Martić, Radava
AU  - Kotur-Stevuljević, Jelena
AU  - Malenović, Anđelija
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Krajišnik, Danina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4200
AB  - Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extra- cellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun protection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The phys- icochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emul- sion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.
PB  - SAGE Publications Inc.
T2  - Natural Product Communications
T1  - Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment
VL  - 17
IS  - 7
DO  - 10.1177/1934578X221112811
ER  - 

@article{
author = "Martić, Radava and Kotur-Stevuljević, Jelena and Malenović, Anđelija and Ušjak, Ljuboš and Petrović, Silvana and Čalija, Bojan and Milić, Jela and Krajišnik, Danina",
year = "2022",
abstract = "Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extra- cellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun protection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The phys- icochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emul- sion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.",
publisher = "SAGE Publications Inc.",
journal = "Natural Product Communications",
title = "Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment",
volume = "17",
number = "7",
doi = "10.1177/1934578X221112811"
}

Martić, R., Kotur-Stevuljević, J., Malenović, A., Ušjak, L., Petrović, S., Čalija, B., Milić, J.,& Krajišnik, D.. (2022). Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment. in Natural Product Communications
SAGE Publications Inc.., 17(7).
https://doi.org/10.1177/1934578X221112811

Martić R, Kotur-Stevuljević J, Malenović A, Ušjak L, Petrović S, Čalija B, Milić J, Krajišnik D. Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment. in Natural Product Communications. 2022;17(7).
doi:10.1177/1934578X221112811 .

Martić, Radava, Kotur-Stevuljević, Jelena, Malenović, Anđelija, Ušjak, Ljuboš, Petrović, Silvana, Čalija, Bojan, Milić, Jela, Krajišnik, Danina, "Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment" in Natural Product Communications, 17, no. 7 (2022),
https://doi.org/10.1177/1934578X221112811 . .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4585
AB  - Chamaecytisus heuffelii subsp. jankae (Velen.) Niketić (Fabaceae) is a dwarfish shrub,
which range includes eastern part of the Balkan Peninsula (Albania, Bulgaria, Serbia) (1).
The aim of the investigation was to chemically characterize aerial flowering parts of this
plant: the dried methanol extract (TM), its alkaloid (AF) and non-alkaloid (NAF) fractions,
and to assess their ability and the ability of detected flavonoids to inhibit enzymes
acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), in vitro in the Ellman
spectrophotometric test. Plant material was collected in eastern Serbia (Jelašnička Klisura
gorge), dried, powdered and extracted with methanol, after dichloromethane pre-extraction.
Using solvents of different polarities and pH value, AF (yield 14.35%) and NAF (yield
57.78%) were obtained from TM. By LC-MS analysis, flavonoids luteolin-8-C-
glucosylpentoside (159.81 mg/g), genistin (22.38 mg/g), vitexin (6.32 mg/g), rutin (4.25
mg/g), and genistein (0.86 mg/g) were detected and quantified in TM, while luteolin-8-C-
glucosylpentoside (212.70 mg/g), rutin (1.38 mg/g), and genistin (1.03 mg/g) in NAF. Using
GC-FID-MS analysis, quinolizidine alkaloids sparteine, 17-oksosparteine, and lupanine were
identified in AF. TM and NAF exhibited significant and comparable anti-cholinesterase
activity (IC50AChE 0.54 and 0.72 mg/mL; IC50BuChE 0.45 and 0.68 mg/mL). All detected
flavonoids evinced the ability to inhibit enzymes; the most active were genistein and vitexin
(IC50AChE 54.75 and 88.35 μg/mL; IC 50BuChE 27.83 and 49.77 μg/mL). Considering the
quinolizidine alkaloids toxicity and AF poor anti-cholinesterase activity, it might be
concluded, regarding safety profile and medical potential, that the fraction of the herb
methanol extract, flavonoid-rich and alkaloid-free (NAF), is good candidate for further
research.
AB  - Chamaecytisus heuffelii subsp. jankae (Velen.) Niketić (Fabaceae) je polužbun, koji
raste na području istočnog dela Balkanskog poluostrva (Albanija, Bugarska, Srbija) (1). Cilj
istraživanja bio je da se izvrši hemijska karakterizacija nadzemnih delova u cvetu ove biljke:
suvog metanolnog ekstrakta (TM), njegove alkaloidne (AF) i frakcije bez alkaloida (NAF), i da
se in vitro, u Ellmanov-om spektrofotomerijskom testu, ispita njihova sposobnost, kao i
sposobnost detektovanih flavonoida da inhibiraju enzime acetilholinesterazu (AChE) i
butirilholinesterazu (BChE). Biljni materijal je sakupljen u istočnoj Srbiji (Jelašnička klisura),
osušen, samleven i ekstrahovan metanolom, nakon pre-ekstrakcije dihlormetanom.
Primenom rastvarača različite polarnosti uz promenu pH vrednosti, iz TM su dobijene AF
(prinos 14,35%) i NAF (prinos 57,78%). LC-MS analizom, u TM detektovani su i
kvanitikovani flavonoidi luteolin-8-C-glukozilpentozid (159,81 mg/g), genistin (22,38 mg/g),
viteksin (6,32 mg/g) i rutin (4,25 mg/g) i genistein (0,86 mg/g), a u NAF luteolin-8-C-
glukozilpentozid (212,70 mg/g), rutin (1,38 mg/g) i genistin (1,03 mg/g). GC-FID-MS
analizom, u AF identifikovani su hinolizidinski alkaloidi spartein, 17-oksospartein i lupanin.
TM i NAF ispoljili su značajnu i uporedivu antiholinesteraznu aktivnost (IC50AChE 0,54 i 0,72
mg/mL; IC 50BChE 0,45 i 0,68 mg/mL). Svi detektovani flavonoidi su pokazali sposobnost da
inhibiraju enzime; najaktivniji su bili genistein i viteksin (IC 50AChE 54,75 i 88,35 μg/mL;
IC 50BuChE 27,83 i 49,77 μg/mL). Uzimajući u obzir toksič nost hinolizidinskih alkaloida i slabu
antiholinesteraznu aktivnost AF, mož e se zaključ iti da je frakcija metanolnog ekstrakata
herbe bogata flavonoidima i oslobođena alkaloida (NAF), u pogledu bezbednosnog profila i
lekovitog potencijala dobar kandidat za dalja ispitivanja.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Secondary metabolites and anti-cholinesterase activity of Chamaecytisus heuffelii subsp. jankae aerial flowering parts
T1  - Sekundarni metaboliti i antiholinesterazna aktivnost herbe Chamaecytisus heuffelii subsp. jankae
VL  - 72
IS  - 4 suplement
SP  - S471
EP  - S472
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4585
ER  - 

@conference{
author = "Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "Chamaecytisus heuffelii subsp. jankae (Velen.) Niketić (Fabaceae) is a dwarfish shrub,
which range includes eastern part of the Balkan Peninsula (Albania, Bulgaria, Serbia) (1).
The aim of the investigation was to chemically characterize aerial flowering parts of this
plant: the dried methanol extract (TM), its alkaloid (AF) and non-alkaloid (NAF) fractions,
and to assess their ability and the ability of detected flavonoids to inhibit enzymes
acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), in vitro in the Ellman
spectrophotometric test. Plant material was collected in eastern Serbia (Jelašnička Klisura
gorge), dried, powdered and extracted with methanol, after dichloromethane pre-extraction.
Using solvents of different polarities and pH value, AF (yield 14.35%) and NAF (yield
57.78%) were obtained from TM. By LC-MS analysis, flavonoids luteolin-8-C-
glucosylpentoside (159.81 mg/g), genistin (22.38 mg/g), vitexin (6.32 mg/g), rutin (4.25
mg/g), and genistein (0.86 mg/g) were detected and quantified in TM, while luteolin-8-C-
glucosylpentoside (212.70 mg/g), rutin (1.38 mg/g), and genistin (1.03 mg/g) in NAF. Using
GC-FID-MS analysis, quinolizidine alkaloids sparteine, 17-oksosparteine, and lupanine were
identified in AF. TM and NAF exhibited significant and comparable anti-cholinesterase
activity (IC50AChE 0.54 and 0.72 mg/mL; IC50BuChE 0.45 and 0.68 mg/mL). All detected
flavonoids evinced the ability to inhibit enzymes; the most active were genistein and vitexin
(IC50AChE 54.75 and 88.35 μg/mL; IC 50BuChE 27.83 and 49.77 μg/mL). Considering the
quinolizidine alkaloids toxicity and AF poor anti-cholinesterase activity, it might be
concluded, regarding safety profile and medical potential, that the fraction of the herb
methanol extract, flavonoid-rich and alkaloid-free (NAF), is good candidate for further
research., Chamaecytisus heuffelii subsp. jankae (Velen.) Niketić (Fabaceae) je polužbun, koji
raste na području istočnog dela Balkanskog poluostrva (Albanija, Bugarska, Srbija) (1). Cilj
istraživanja bio je da se izvrši hemijska karakterizacija nadzemnih delova u cvetu ove biljke:
suvog metanolnog ekstrakta (TM), njegove alkaloidne (AF) i frakcije bez alkaloida (NAF), i da
se in vitro, u Ellmanov-om spektrofotomerijskom testu, ispita njihova sposobnost, kao i
sposobnost detektovanih flavonoida da inhibiraju enzime acetilholinesterazu (AChE) i
butirilholinesterazu (BChE). Biljni materijal je sakupljen u istočnoj Srbiji (Jelašnička klisura),
osušen, samleven i ekstrahovan metanolom, nakon pre-ekstrakcije dihlormetanom.
Primenom rastvarača različite polarnosti uz promenu pH vrednosti, iz TM su dobijene AF
(prinos 14,35%) i NAF (prinos 57,78%). LC-MS analizom, u TM detektovani su i
kvanitikovani flavonoidi luteolin-8-C-glukozilpentozid (159,81 mg/g), genistin (22,38 mg/g),
viteksin (6,32 mg/g) i rutin (4,25 mg/g) i genistein (0,86 mg/g), a u NAF luteolin-8-C-
glukozilpentozid (212,70 mg/g), rutin (1,38 mg/g) i genistin (1,03 mg/g). GC-FID-MS
analizom, u AF identifikovani su hinolizidinski alkaloidi spartein, 17-oksospartein i lupanin.
TM i NAF ispoljili su značajnu i uporedivu antiholinesteraznu aktivnost (IC50AChE 0,54 i 0,72
mg/mL; IC 50BChE 0,45 i 0,68 mg/mL). Svi detektovani flavonoidi su pokazali sposobnost da
inhibiraju enzime; najaktivniji su bili genistein i viteksin (IC 50AChE 54,75 i 88,35 μg/mL;
IC 50BuChE 27,83 i 49,77 μg/mL). Uzimajući u obzir toksič nost hinolizidinskih alkaloida i slabu
antiholinesteraznu aktivnost AF, mož e se zaključ iti da je frakcija metanolnog ekstrakata
herbe bogata flavonoidima i oslobođena alkaloida (NAF), u pogledu bezbednosnog profila i
lekovitog potencijala dobar kandidat za dalja ispitivanja.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Secondary metabolites and anti-cholinesterase activity of Chamaecytisus heuffelii subsp. jankae aerial flowering parts, Sekundarni metaboliti i antiholinesterazna aktivnost herbe Chamaecytisus heuffelii subsp. jankae",
volume = "72",
number = "4 suplement",
pages = "S471-S472",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4585"
}

Milutinović, V., Niketić, M.,& Petrović, S.. (2022). Secondary metabolites and anti-cholinesterase activity of Chamaecytisus heuffelii subsp. jankae aerial flowering parts. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S471-S472.
https://hdl.handle.net/21.15107/rcub_farfar_4585

Milutinović V, Niketić M, Petrović S. Secondary metabolites and anti-cholinesterase activity of Chamaecytisus heuffelii subsp. jankae aerial flowering parts. in Arhiv za farmaciju. 2022;72(4 suplement):S471-S472.
https://hdl.handle.net/21.15107/rcub_farfar_4585 .

Milutinović, Violeta, Niketić, Marjan, Petrović, Silvana, "Secondary metabolites and anti-cholinesterase activity of Chamaecytisus heuffelii subsp. jankae aerial flowering parts" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S471-S472,
https://hdl.handle.net/21.15107/rcub_farfar_4585 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4505
AB  - The genus Hieracium L. s. str. (Cichorieae, Asteraceae) is one of the most taxonomically complex genera of flowering plants. Chemosystematic significance of triterpenes especially for Asteraceae genera is scarcely assessed. The subject of this research are 28 Hieracium species from Balkan Peninsula (mostly collected at Mt Durmitor, Montenegro): 12 principal belonging to sections Pannosa, Naegeliana, Drepanoidea and Villosa, and 16 hybridogenous originated from the species from those four sections and Glauciformia, Hieracium, Prenanthoidea and/or Italica. Previously, in dried dichloromethane extracts of flowering aerial plant parts, α-amyrin and α-amyrin acetate, as well as β-amyrin, β-amyrin acetate and/or lupeol acetate were identified and quantified using GC-FID-MS. The aim was to evaluate chemosystematic significance of these five triterpenes using multivariate statistical methods (principal components analysis-PCA and non-metric multidimensional scaling-nMDS). Statistical analysis supported the current taxonomical classification of the investigated species, despite overlapping of certain groups. In PCA, all five triterpenes significantly contributed to the differences between the species. Moreover, lupeol acetate could be a significant chemosystematic marker for the most of principal species belonging to the section Pannosa, as well as for some hybridogenous species between H. gymnocephalum Gris. ex Pant. and the members from the other sections.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Chemosystematic significance of triterpenes from dichloromethane extracts of 28 Hieracium L. species from the Balkan Peninsula
SP  - 185
EP  - 185
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4505
ER  - 

@conference{
author = "Milutinović, Violeta and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "The genus Hieracium L. s. str. (Cichorieae, Asteraceae) is one of the most taxonomically complex genera of flowering plants. Chemosystematic significance of triterpenes especially for Asteraceae genera is scarcely assessed. The subject of this research are 28 Hieracium species from Balkan Peninsula (mostly collected at Mt Durmitor, Montenegro): 12 principal belonging to sections Pannosa, Naegeliana, Drepanoidea and Villosa, and 16 hybridogenous originated from the species from those four sections and Glauciformia, Hieracium, Prenanthoidea and/or Italica. Previously, in dried dichloromethane extracts of flowering aerial plant parts, α-amyrin and α-amyrin acetate, as well as β-amyrin, β-amyrin acetate and/or lupeol acetate were identified and quantified using GC-FID-MS. The aim was to evaluate chemosystematic significance of these five triterpenes using multivariate statistical methods (principal components analysis-PCA and non-metric multidimensional scaling-nMDS). Statistical analysis supported the current taxonomical classification of the investigated species, despite overlapping of certain groups. In PCA, all five triterpenes significantly contributed to the differences between the species. Moreover, lupeol acetate could be a significant chemosystematic marker for the most of principal species belonging to the section Pannosa, as well as for some hybridogenous species between H. gymnocephalum Gris. ex Pant. and the members from the other sections.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Chemosystematic significance of triterpenes from dichloromethane extracts of 28 Hieracium L. species from the Balkan Peninsula",
pages = "185-185",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4505"
}

Milutinović, V., Ušjak, L., Niketić, M.,& Petrović, S.. (2022). Chemosystematic significance of triterpenes from dichloromethane extracts of 28 Hieracium L. species from the Balkan Peninsula. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 185-185.
https://hdl.handle.net/21.15107/rcub_farfar_4505

Milutinović V, Ušjak L, Niketić M, Petrović S. Chemosystematic significance of triterpenes from dichloromethane extracts of 28 Hieracium L. species from the Balkan Peninsula. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:185-185.
https://hdl.handle.net/21.15107/rcub_farfar_4505 .

Milutinović, Violeta, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Chemosystematic significance of triterpenes from dichloromethane extracts of 28 Hieracium L. species from the Balkan Peninsula" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):185-185,
https://hdl.handle.net/21.15107/rcub_farfar_4505 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4504
AB  - The wood-sage (woodland germander) Teucrium scorodonia L. is widespread in European Atlantic coast and in the western part of Central Europe, with some isolated populations in Mediterranean. Recently discovered population on the territory of Serbia, in the valley of Štavica river (vicinity of Loznica), represents the most eastern enclave in the species range. Preliminary chemical analysis of aerial flowering parts and its dried ethanol and hydroethanol (70% v/v) extracts, and assessment of antioxidant potential of selected (hydroethanol) extract were performed. Using appropriate spectrophotometrical methods, total polyphenols in herb (3.48%, expressed as pyrogallol), ethanol and hydroethanol extracts (100.05 and 96.65 mg gallic acid equivalents - GAE/g), tannins in herb (0.96%, expressed as pyrogallol), ethanol and hydroethanol extracts (11.86 and 12.31 mg GAE/g), flavonoids in herb, ethanol and hydroethanol extracts (0.19%, 0.83% and 0.74%, expressed as hyperoside) were quantified. Also, in ethanol and hydroethanol extracts dihydroxycinnamic acid derivatives were determined (8.96% and 8.37%, expressed as acteoside). By LC-MS, in selected (hydroethanol) extract, four flavonoid and five phenylethanoid glycosides were detected; verbascoside (acteoside) was identified and quantified (4.42%) using external standard. This extract exhibited significant total antioxidant activity (1.37 mmol Fe2+/g) and moderate anti-DPPH potential (SC50=51.28 μg/mL).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Chemical composition and antioxidant potential of Teucrium scorodonia L. (Lamiaceae) from Serbia
SP  - 180
EP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4504
ER  - 

@conference{
author = "Milutinović, Violeta and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "The wood-sage (woodland germander) Teucrium scorodonia L. is widespread in European Atlantic coast and in the western part of Central Europe, with some isolated populations in Mediterranean. Recently discovered population on the territory of Serbia, in the valley of Štavica river (vicinity of Loznica), represents the most eastern enclave in the species range. Preliminary chemical analysis of aerial flowering parts and its dried ethanol and hydroethanol (70% v/v) extracts, and assessment of antioxidant potential of selected (hydroethanol) extract were performed. Using appropriate spectrophotometrical methods, total polyphenols in herb (3.48%, expressed as pyrogallol), ethanol and hydroethanol extracts (100.05 and 96.65 mg gallic acid equivalents - GAE/g), tannins in herb (0.96%, expressed as pyrogallol), ethanol and hydroethanol extracts (11.86 and 12.31 mg GAE/g), flavonoids in herb, ethanol and hydroethanol extracts (0.19%, 0.83% and 0.74%, expressed as hyperoside) were quantified. Also, in ethanol and hydroethanol extracts dihydroxycinnamic acid derivatives were determined (8.96% and 8.37%, expressed as acteoside). By LC-MS, in selected (hydroethanol) extract, four flavonoid and five phenylethanoid glycosides were detected; verbascoside (acteoside) was identified and quantified (4.42%) using external standard. This extract exhibited significant total antioxidant activity (1.37 mmol Fe2+/g) and moderate anti-DPPH potential (SC50=51.28 μg/mL).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Chemical composition and antioxidant potential of Teucrium scorodonia L. (Lamiaceae) from Serbia",
pages = "180-180",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4504"
}

Milutinović, V., Ušjak, L., Niketić, M.,& Petrović, S.. (2022). Chemical composition and antioxidant potential of Teucrium scorodonia L. (Lamiaceae) from Serbia. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 180-180.
https://hdl.handle.net/21.15107/rcub_farfar_4504

Milutinović V, Ušjak L, Niketić M, Petrović S. Chemical composition and antioxidant potential of Teucrium scorodonia L. (Lamiaceae) from Serbia. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:180-180.
https://hdl.handle.net/21.15107/rcub_farfar_4504 .

Milutinović, Violeta, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Chemical composition and antioxidant potential of Teucrium scorodonia L. (Lamiaceae) from Serbia" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):180-180,
https://hdl.handle.net/21.15107/rcub_farfar_4504 .

TY  - JOUR
AU  - Milutinović, Violeta
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana
AU  - Soković, Marina
AU  - Ćirić, Ana D.
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan S.
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4172
AB  - Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7–2.5 mg/mL MBC=3.4–5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15–0.4 mg/mL MFC=0.3–0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50=148.1 μg/mL for SCH, and 152.3–303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50=46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.
PB  - John Wiley and Sons Inc
T2  - Chemistry and Biodiversity
T1  - Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones
VL  - 19
IS  - 7
SP  - e202200326
DO  - 10.1002/cbdv.202200326
ER  - 

@article{
author = "Milutinović, Violeta and Matić, Ivana Z. and Stanojković, Tatjana and Soković, Marina and Ćirić, Ana D. and Ušjak, Ljuboš and Niketić, Marjan S. and Petrović, Silvana",
year = "2022",
abstract = "Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7–2.5 mg/mL MBC=3.4–5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15–0.4 mg/mL MFC=0.3–0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50=148.1 μg/mL for SCH, and 152.3–303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50=46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.",
publisher = "John Wiley and Sons Inc",
journal = "Chemistry and Biodiversity",
title = "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones",
volume = "19",
number = "7",
pages = "e202200326",
doi = "10.1002/cbdv.202200326"
}

Milutinović, V., Matić, I. Z., Stanojković, T., Soković, M., Ćirić, A. D., Ušjak, L., Niketić, M. S.,& Petrović, S.. (2022). Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones. in Chemistry and Biodiversity
John Wiley and Sons Inc., 19(7), e202200326.
https://doi.org/10.1002/cbdv.202200326

Milutinović V, Matić IZ, Stanojković T, Soković M, Ćirić AD, Ušjak L, Niketić MS, Petrović S. Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones. in Chemistry and Biodiversity. 2022;19(7):e202200326.
doi:10.1002/cbdv.202200326 .

Milutinović, Violeta, Matić, Ivana Z., Stanojković, Tatjana, Soković, Marina, Ćirić, Ana D., Ušjak, Ljuboš, Niketić, Marjan S., Petrović, Silvana, "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones" in Chemistry and Biodiversity, 19, no. 7 (2022):e202200326,
https://doi.org/10.1002/cbdv.202200326 . .

TY  - CONF
AU  - Kukić-Marković, Jelena
AU  - Milutinović, Violeta
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4423
AB  - Stachys milanii Petrović (Lamiaceae) is an endemic of central and eastern part of Balkan Peninsula, growing mainly in river valleys on semi-saline soils, often along the edge of cereal fields and abandoned arable lands. It is a critically endangered and strictly protected species in Serbia, with just one proven habitat (1). So far it was investigated on herb essential oil composition and antimicrobial activity, and on fatty acid composition of seeds (2). 
Aim of this work was to investigate antiradical potential and phenolic constituents of dry methanol herb extract. Aerial flowering parts (herb) of S. milanii were collected in North Macedonia (Ovče Pole). Powdered, dried plant material was extracted by bimaceration with chloroform, and then with methanol. Obtained dry methanol extract was used for further analysis. Using appropriate spectrophotometric procedure strong, concentration-dependant anti-DPPH activity of extract was observed (SC50=34.15 μg/mL). Also spectrophotometrically, using Folin-Ciocalteu reagent, significant total phenolics content (0,47 mg gallic acid/mg dry extract) was determined. In TLC-DPPH test observed yellow zones indicated presence of few compounds which strongly neutralized purple DPPH radical. LC-MS analysis revealed presence of one phenolic acid, four phenylethanol glycosides, eight flavonoid glycosides derivatives of 8-hydroxyflavones, and one apigenin glycoside. Using commercial standards in extract were identified and quantified: chlorogenic acid (1.63%) and acteoside (2.65%), as the main phenylethanoid. In 8-hydroxyflavones fraction six hypolaetine and two isoscutellarein derivatives were present; using compounds previously isolated from S. alpina subsp. dinarica Murb. herb, 4'-O-methylhypolaetine-7-O-[6'''-acetylallosyl(1®2)]-glucopyranoside and isoscutellarein-7-O-[6'''-acetylallosyl(1®2)]-glucopyranoside were identified, representing the most prominent flavonoids in investigated extract, respectively.  


References
1. Niketić M, Diklić N. Stachys milanii Petrović. In: Stevanović V. (Ed.) Red Book of Flora of Serbia 1 - Extinct and Critically Endangered Taxa. Belgrade: Ministry for the Protection of Natural Resources and the Environment, Faculty of Biology, University of Belgrade, Institution for Protection of Nature of the Republic of Serbia. 1999; pp. 211-3, 466-7.
2. Stojanovic G, Ligon AP, Smelcerovic A, Lazarevic J, Spiteller M, Palic R. Fatty acids of Stachys milanii seeds. Chem Nat Comp 2007;43(4):380-3.


Acknowledgements
This research was funded by the Ministry of Education, Science and Technological Development, Republic of Serbia through Grant Agreement with University of Belgrade-Faculty of Pharmacy No: 451-03-68/2022-14/200161.
AB  - Stachys milanii Petrović (Lamiaceae) je endemit centralnog i istočnog dela Balkanskog
poluostrva koji raste pretežno u dolinama reka na poluslanim zemljištima, često i po obodu
žitnih polja i po napuštenim oranicama. U Republici Srbiji je kritično ugrožena i strogo
zaštićena vrsta, sa samo jednim zabeleženim staništem (1). Biljka je do sada ispitivana u
pogledu sastava i antimikrobne aktivnosti etarskog ulja herbe i kompozije masnih kiselina
semena (2). Cilj ovog rada bio je da se ispitaju antiradikalski potencijal i fenolni sastojci
suvog metanolnog ekstrakta herbe. Nadzemni delovi S. milanii u cvetu prikupljeni su u
Severnoj Makedoniji (Ovče Pole). Sprašen, osušen biljni materijal je ekstrahovan posupkom
bimaceracije najpre hloroformom, a zatim metanolom. Dobijeni suvi metanolni ekstrakt
korišćen je za dalju analizu. Odgovarajućim spektrofotometrijskim postupkom utvrđena je
snažna, koncentraciono-zavisna anti-DPPH aktivnost ekstrakta (SC50=34,15 μg/mL).
Spektrofotometrijski, korišćenjem Folin-Ciocalteu reagensa, utvrđen je i značajan sadržaj
ukupnih polifenola (0,47 mg galne kiseline/mg suvog ekstrakta). U TLC-DPPH testu uočene
žute zone ukazale su na prisustvo većeg broja jedinjenja koja snažno neutrališu ljubičasti
DPPH-radikal. LC-MS analizom u ekstraktu su detektovani jedna fenolkarboksilna kiselina,
četiri feniletanska heterozida, osam flavonoidnih heterozida derivata 8-hidroksiflavona i
jedan heterozid apigenina. Korišćenjem komercijalnih standarda identifikovni su i
kvantifikovani hlorogenska kiselina (1,63%) i akteozid (2,65%), kao glavni feniletanski
heterozid. Frakciju derivata 8-hidroksiflavona činilo je šest heterozida hipolaetina i dva
heterozida izoskutelareina; korišćenjem jedinjenja prethodno izolovanih iz herbe S. alpina
subsp. dinarica Murb., identifikovani su 4'-O-metilhipolaetin-7-O-[6'''-acetilalozil(12)]-
glukopiranozid i izoskutelarein 7-O-[6'''-acetilalozil(12)]-glukopiranozid, koji su ujedno
predstavljali i najzastupljnije flavonoide u ekstraktu, redom.
PB  - Pharmaceutical Association of Serbia
C3  - Arhiv za farmaciju
T1  - Antiradical activity and phenolic constituents of Stachys milanii aerial flowering parts methanol extract
T1  - Antiradikalska aktivnost i fenolni sastojci metanolnog ekstrakta herbe Stachys milanii
VL  - 72
IS  - 4 suplement
SP  - S463
EP  - S464
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4423
ER  - 

@conference{
author = "Kukić-Marković, Jelena and Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "Stachys milanii Petrović (Lamiaceae) is an endemic of central and eastern part of Balkan Peninsula, growing mainly in river valleys on semi-saline soils, often along the edge of cereal fields and abandoned arable lands. It is a critically endangered and strictly protected species in Serbia, with just one proven habitat (1). So far it was investigated on herb essential oil composition and antimicrobial activity, and on fatty acid composition of seeds (2). 
Aim of this work was to investigate antiradical potential and phenolic constituents of dry methanol herb extract. Aerial flowering parts (herb) of S. milanii were collected in North Macedonia (Ovče Pole). Powdered, dried plant material was extracted by bimaceration with chloroform, and then with methanol. Obtained dry methanol extract was used for further analysis. Using appropriate spectrophotometric procedure strong, concentration-dependant anti-DPPH activity of extract was observed (SC50=34.15 μg/mL). Also spectrophotometrically, using Folin-Ciocalteu reagent, significant total phenolics content (0,47 mg gallic acid/mg dry extract) was determined. In TLC-DPPH test observed yellow zones indicated presence of few compounds which strongly neutralized purple DPPH radical. LC-MS analysis revealed presence of one phenolic acid, four phenylethanol glycosides, eight flavonoid glycosides derivatives of 8-hydroxyflavones, and one apigenin glycoside. Using commercial standards in extract were identified and quantified: chlorogenic acid (1.63%) and acteoside (2.65%), as the main phenylethanoid. In 8-hydroxyflavones fraction six hypolaetine and two isoscutellarein derivatives were present; using compounds previously isolated from S. alpina subsp. dinarica Murb. herb, 4'-O-methylhypolaetine-7-O-[6'''-acetylallosyl(1®2)]-glucopyranoside and isoscutellarein-7-O-[6'''-acetylallosyl(1®2)]-glucopyranoside were identified, representing the most prominent flavonoids in investigated extract, respectively.  


References
1. Niketić M, Diklić N. Stachys milanii Petrović. In: Stevanović V. (Ed.) Red Book of Flora of Serbia 1 - Extinct and Critically Endangered Taxa. Belgrade: Ministry for the Protection of Natural Resources and the Environment, Faculty of Biology, University of Belgrade, Institution for Protection of Nature of the Republic of Serbia. 1999; pp. 211-3, 466-7.
2. Stojanovic G, Ligon AP, Smelcerovic A, Lazarevic J, Spiteller M, Palic R. Fatty acids of Stachys milanii seeds. Chem Nat Comp 2007;43(4):380-3.


Acknowledgements
This research was funded by the Ministry of Education, Science and Technological Development, Republic of Serbia through Grant Agreement with University of Belgrade-Faculty of Pharmacy No: 451-03-68/2022-14/200161., Stachys milanii Petrović (Lamiaceae) je endemit centralnog i istočnog dela Balkanskog
poluostrva koji raste pretežno u dolinama reka na poluslanim zemljištima, često i po obodu
žitnih polja i po napuštenim oranicama. U Republici Srbiji je kritično ugrožena i strogo
zaštićena vrsta, sa samo jednim zabeleženim staništem (1). Biljka je do sada ispitivana u
pogledu sastava i antimikrobne aktivnosti etarskog ulja herbe i kompozije masnih kiselina
semena (2). Cilj ovog rada bio je da se ispitaju antiradikalski potencijal i fenolni sastojci
suvog metanolnog ekstrakta herbe. Nadzemni delovi S. milanii u cvetu prikupljeni su u
Severnoj Makedoniji (Ovče Pole). Sprašen, osušen biljni materijal je ekstrahovan posupkom
bimaceracije najpre hloroformom, a zatim metanolom. Dobijeni suvi metanolni ekstrakt
korišćen je za dalju analizu. Odgovarajućim spektrofotometrijskim postupkom utvrđena je
snažna, koncentraciono-zavisna anti-DPPH aktivnost ekstrakta (SC50=34,15 μg/mL).
Spektrofotometrijski, korišćenjem Folin-Ciocalteu reagensa, utvrđen je i značajan sadržaj
ukupnih polifenola (0,47 mg galne kiseline/mg suvog ekstrakta). U TLC-DPPH testu uočene
žute zone ukazale su na prisustvo većeg broja jedinjenja koja snažno neutrališu ljubičasti
DPPH-radikal. LC-MS analizom u ekstraktu su detektovani jedna fenolkarboksilna kiselina,
četiri feniletanska heterozida, osam flavonoidnih heterozida derivata 8-hidroksiflavona i
jedan heterozid apigenina. Korišćenjem komercijalnih standarda identifikovni su i
kvantifikovani hlorogenska kiselina (1,63%) i akteozid (2,65%), kao glavni feniletanski
heterozid. Frakciju derivata 8-hidroksiflavona činilo je šest heterozida hipolaetina i dva
heterozida izoskutelareina; korišćenjem jedinjenja prethodno izolovanih iz herbe S. alpina
subsp. dinarica Murb., identifikovani su 4'-O-metilhipolaetin-7-O-[6'''-acetilalozil(12)]-
glukopiranozid i izoskutelarein 7-O-[6'''-acetilalozil(12)]-glukopiranozid, koji su ujedno
predstavljali i najzastupljnije flavonoide u ekstraktu, redom.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za farmaciju",
title = "Antiradical activity and phenolic constituents of Stachys milanii aerial flowering parts methanol extract, Antiradikalska aktivnost i fenolni sastojci metanolnog ekstrakta herbe Stachys milanii",
volume = "72",
number = "4 suplement",
pages = "S463-S464",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4423"
}

Kukić-Marković, J., Milutinović, V., Niketić, M.,& Petrović, S.. (2022). Antiradical activity and phenolic constituents of Stachys milanii aerial flowering parts methanol extract. in Arhiv za farmaciju
Pharmaceutical Association of Serbia., 72(4 suplement), S463-S464.
https://hdl.handle.net/21.15107/rcub_farfar_4423

Kukić-Marković J, Milutinović V, Niketić M, Petrović S. Antiradical activity and phenolic constituents of Stachys milanii aerial flowering parts methanol extract. in Arhiv za farmaciju. 2022;72(4 suplement):S463-S464.
https://hdl.handle.net/21.15107/rcub_farfar_4423 .

Kukić-Marković, Jelena, Milutinović, Violeta, Niketić, Marjan, Petrović, Silvana, "Antiradical activity and phenolic constituents of Stachys milanii aerial flowering parts methanol extract" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S463-S464,
https://hdl.handle.net/21.15107/rcub_farfar_4423 .

TY  - CONF
AU  - Jovanović, Aleksandar
AU  - Stefanović-Vojinović, Jelena
AU  - Kukić-Marković, Jelena
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Tadić, Ivana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4424
AB  - The use of medicinal plants has significant impact on human health (1,2). Approximately 13.000 medicinal plants are used worldwide (1), and in developing countries 65-80% of the population uses them for medicinal purposes (2). 
The aim of the study was to examine the awareness of patients in the city of Belgrade about herbal products. Quantitative, non-experimental research was conducted using a purpose-built questionnaire in public pharmacies during February 2020.
The survey involved 954 respondents, mostly women (66.5%); 39.1% of respondents aged 20-40 years. Most of the respondents were employed (65.2%), with secondary school education (45.2%). The vast majority (97.3%) used medicinal plants/herbal products, mostly occasionally, as needed (38.4%). The main motive for their use was health improvement (75.2%), while 16.2% used them to treat diseases. The primary sources of information on medicinal plants/herbal products are pharmacists (45.1%) and the media (24.2%). About a third of the respondents (37.3%) stated that they knew the difference between herbal medicinal products and dietary supplements. 52.9% of respondents claimed that herbal medicinal products are effective, but less than synthetic drugs, while 61.5% claimed that they are safe, but with certain side effects. A third of respondents (32.9%) had difficulty understanding instructions for use and they needed additional explanations. Majority (70.6%) received advice from a pharmacist when buying a herbal product.
Pharmacists are health professionals who have adequate knowledge of medicinal plants/herbal products and have a key role in advising patients in order to implement the proper use of herbal medicinal and other herbal products.

References
1. Capasso F, Gaginella TS, Grandolini G, Izzo AA. Phytotherapy: a quick reference to herbal medicine. Springer Science & Business Media. 2003.
2. Palhares RM, Gonçalves Drummond M, dos Santos Alves Figueiredo Brasil B, Pereira Cosenza G, das Graças Lins Brandão M, Oliveira G. Medicinal plants recommended by the World Health Organization: DNA barcode identification associated with chemical analyses guarantees their quality. PloS one. 2015;10(5), e0127866.

Acknowledgements
This research was conducted with help and support of Pharmacy „Lilly Drogerie“ and funded by the Ministry of Education, Science and Technological Development, Republic of Serbia through Grant Agreement with University of Belgrade-Faculty of Pharmacy No: 451-03-68/2022-14/200161.
AB  - Primena lekovitih biljaka ima značajan uticaj na ljudsko zdravlje (1,2). U svetu se
koristi oko 13 000 lekovitih biljaka (1), a u zemljama u razvoju 65-80% populacije ih
primenjuje u medicinske svrhe (2). Cilj rada bio je ispitivanje informisanosti pacijenata na
teritoriji grada Beograda o biljnim proizvodima. Sprovedeno je kvantitativno,
neeksperimentalno istraživanje pomoću namenski kreiranog upitnika u javnim apotekama
tokom februara 2020. godine. U istraživanju je učestvovalo 954 ispitanika, od koji 66,5%
ženskog pola; 39,1% starosti 20-40 godina. Većina je bila zaposlena (65,2%), sa
srednjoškolskim obrazovanjem (45,2%). Najveći broj ispitanika (97,3%) je koristio lekovite
biljke/biljne proizvode i to uglavnom povremeno, po potrebi (38,4%). Glavni motiv za
njihovo korišćenje bio je poboljšanje zdravlja (75,2%), dok ih je 16,2% koristilo za lečenje.
Ispitanici su se o delovanju i upotrebi lekovitih biljaka/biljnih proizvoda uglavnom
informisali od farmaceuta u apotekama (45,1%) i putem medija (24,2%). Oko trećine
ispitanika (37,3%) je navelo da zna razliku između biljnih lekovitih proizvoda i dijetetskih
suplemenata. Da su biljni lekoviti proizvodi efikasni, ali manje od sintetskih lekova, smatralo
je 52,9% ispitanika, dok je 61,5% smatralo da su oni bezbedni za upotrebu, ali sa mogućim
neželjenim efektima. Trećina anketiranih (32,9%) imala je poteškoće da razume uputstva za
upotrebu biljnih proizvoda, te su im bila potrebna dodatna objašnjenja. Najveći deo
ispitanika (70,6%) dobijao je savet farmaceuta pri kupovini biljnog proizvoda. Farmaceuti su
zdravstveni profesionalci koji poseduju adekvatna znanja o lekovitim biljkama/biljnim
proizvodima i imaju ključnu ulogu u savetovanju pacijenata u cilju sprovođenja pravilne
primene biljnih lekovitih i drugih biljnih proizvoda.
C3  - Arhiv za farmaciju
T1  - Awareness of patients in the city of Belgrade about herbal products
T1  - Informisanost pacijenata na teritoriji grada Beograda o biljnim proizvodima
VL  - 72
IS  - 4 suplement
SP  - S473
EP  - S474
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4424
ER  - 

@conference{
author = "Jovanović, Aleksandar and Stefanović-Vojinović, Jelena and Kukić-Marković, Jelena and Petrović, Silvana and Drobac, Milica and Tadić, Ivana",
year = "2022",
abstract = "The use of medicinal plants has significant impact on human health (1,2). Approximately 13.000 medicinal plants are used worldwide (1), and in developing countries 65-80% of the population uses them for medicinal purposes (2). 
The aim of the study was to examine the awareness of patients in the city of Belgrade about herbal products. Quantitative, non-experimental research was conducted using a purpose-built questionnaire in public pharmacies during February 2020.
The survey involved 954 respondents, mostly women (66.5%); 39.1% of respondents aged 20-40 years. Most of the respondents were employed (65.2%), with secondary school education (45.2%). The vast majority (97.3%) used medicinal plants/herbal products, mostly occasionally, as needed (38.4%). The main motive for their use was health improvement (75.2%), while 16.2% used them to treat diseases. The primary sources of information on medicinal plants/herbal products are pharmacists (45.1%) and the media (24.2%). About a third of the respondents (37.3%) stated that they knew the difference between herbal medicinal products and dietary supplements. 52.9% of respondents claimed that herbal medicinal products are effective, but less than synthetic drugs, while 61.5% claimed that they are safe, but with certain side effects. A third of respondents (32.9%) had difficulty understanding instructions for use and they needed additional explanations. Majority (70.6%) received advice from a pharmacist when buying a herbal product.
Pharmacists are health professionals who have adequate knowledge of medicinal plants/herbal products and have a key role in advising patients in order to implement the proper use of herbal medicinal and other herbal products.

References
1. Capasso F, Gaginella TS, Grandolini G, Izzo AA. Phytotherapy: a quick reference to herbal medicine. Springer Science & Business Media. 2003.
2. Palhares RM, Gonçalves Drummond M, dos Santos Alves Figueiredo Brasil B, Pereira Cosenza G, das Graças Lins Brandão M, Oliveira G. Medicinal plants recommended by the World Health Organization: DNA barcode identification associated with chemical analyses guarantees their quality. PloS one. 2015;10(5), e0127866.

Acknowledgements
This research was conducted with help and support of Pharmacy „Lilly Drogerie“ and funded by the Ministry of Education, Science and Technological Development, Republic of Serbia through Grant Agreement with University of Belgrade-Faculty of Pharmacy No: 451-03-68/2022-14/200161., Primena lekovitih biljaka ima značajan uticaj na ljudsko zdravlje (1,2). U svetu se
koristi oko 13 000 lekovitih biljaka (1), a u zemljama u razvoju 65-80% populacije ih
primenjuje u medicinske svrhe (2). Cilj rada bio je ispitivanje informisanosti pacijenata na
teritoriji grada Beograda o biljnim proizvodima. Sprovedeno je kvantitativno,
neeksperimentalno istraživanje pomoću namenski kreiranog upitnika u javnim apotekama
tokom februara 2020. godine. U istraživanju je učestvovalo 954 ispitanika, od koji 66,5%
ženskog pola; 39,1% starosti 20-40 godina. Većina je bila zaposlena (65,2%), sa
srednjoškolskim obrazovanjem (45,2%). Najveći broj ispitanika (97,3%) je koristio lekovite
biljke/biljne proizvode i to uglavnom povremeno, po potrebi (38,4%). Glavni motiv za
njihovo korišćenje bio je poboljšanje zdravlja (75,2%), dok ih je 16,2% koristilo za lečenje.
Ispitanici su se o delovanju i upotrebi lekovitih biljaka/biljnih proizvoda uglavnom
informisali od farmaceuta u apotekama (45,1%) i putem medija (24,2%). Oko trećine
ispitanika (37,3%) je navelo da zna razliku između biljnih lekovitih proizvoda i dijetetskih
suplemenata. Da su biljni lekoviti proizvodi efikasni, ali manje od sintetskih lekova, smatralo
je 52,9% ispitanika, dok je 61,5% smatralo da su oni bezbedni za upotrebu, ali sa mogućim
neželjenim efektima. Trećina anketiranih (32,9%) imala je poteškoće da razume uputstva za
upotrebu biljnih proizvoda, te su im bila potrebna dodatna objašnjenja. Najveći deo
ispitanika (70,6%) dobijao je savet farmaceuta pri kupovini biljnog proizvoda. Farmaceuti su
zdravstveni profesionalci koji poseduju adekvatna znanja o lekovitim biljkama/biljnim
proizvodima i imaju ključnu ulogu u savetovanju pacijenata u cilju sprovođenja pravilne
primene biljnih lekovitih i drugih biljnih proizvoda.",
journal = "Arhiv za farmaciju",
title = "Awareness of patients in the city of Belgrade about herbal products, Informisanost pacijenata na teritoriji grada Beograda o biljnim proizvodima",
volume = "72",
number = "4 suplement",
pages = "S473-S474",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4424"
}

Jovanović, A., Stefanović-Vojinović, J., Kukić-Marković, J., Petrović, S., Drobac, M.,& Tadić, I.. (2022). Awareness of patients in the city of Belgrade about herbal products. in Arhiv za farmaciju, 72(4 suplement), S473-S474.
https://hdl.handle.net/21.15107/rcub_farfar_4424

Jovanović A, Stefanović-Vojinović J, Kukić-Marković J, Petrović S, Drobac M, Tadić I. Awareness of patients in the city of Belgrade about herbal products. in Arhiv za farmaciju. 2022;72(4 suplement):S473-S474.
https://hdl.handle.net/21.15107/rcub_farfar_4424 .

Jovanović, Aleksandar, Stefanović-Vojinović, Jelena, Kukić-Marković, Jelena, Petrović, Silvana, Drobac, Milica, Tadić, Ivana, "Awareness of patients in the city of Belgrade about herbal products" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S473-S474,
https://hdl.handle.net/21.15107/rcub_farfar_4424 .

TY  - JOUR
AU  - Karioti, Anastasia
AU  - Kukić-Marković, Jelena
AU  - Bilia, Anna Rita
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4274
AB  - Aim of this work was the chemical profiling of six Stachys taxa from Balkan Peninsula: S. recta subsp. nitens, S. atherocalyx, S. beckeana, S. zepcensis (all four belonging to S. recta group), S. alpina subsp. dinarica and S. plumosa. Dry ethanol extracts of aerial flowering plant parts were qualitatively and quantitatively characterized for their phenolic composition using the HPLC-DAD-ESI-MS technique. In total, 44 phenolic compounds were detected, belonging to three main classes of constituents: phenolic acids, phenylethanol glycosides (PhGs) and flavonoids. Chlorogenic acid was the main phenolic acid in all samples. Regarding PhGs, in all investigated extracts acteoside and lavandulifolioside were present. In S. recta group, extracts of S. atherocalyx, S. recta subsp. nitens and S. beckeana showed similar pattern considering phenylethanol glycosides, while S. zepcensis contained only the two aforementioned compounds. PhGs profile of S. plumosa extract comprised the same compounds from S. recta group, but it was also characterized by the presence of few high-molecular compounds. PhGs composition of S. alpina subsp. dinarica extract was somewhat similar with those of S. recta group and also with S. plumosa, but still specific by the presence of echinacoside and leucosceptoside A. The main flavonoids in extracts of Stachys recta group as well as in S. alpina subsp. dinarica extract were 8-hydroxyflavone glycosides (7-O-allosylglucosides of isoscutellarein and hypolaetin and their 4′-methyl derivatives). In addition, in the extract of S. alpina subsp. dinarica two luteolin-7-O-hexosides were also present. On the other hand, S. plumosa extract differed considerably containing mainly derivatives of chrysoeriol and apigenin, while 8-hydroxyflavones (isoscutellarein derivatives) were detected in traces. The results confirmed the importance of flavone 7-O-allosylglucosides at the sectional level of Stachys genus.
PB  - Elsevier Ltd
T2  - Biochemical Systematics and Ecology
T1  - Chemical profiling of six Stachys taxa from Balkan Peninsula
VL  - 104
DO  - 10.1016/j.bse.2022.104482
ER  - 

@article{
author = "Karioti, Anastasia and Kukić-Marković, Jelena and Bilia, Anna Rita and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "Aim of this work was the chemical profiling of six Stachys taxa from Balkan Peninsula: S. recta subsp. nitens, S. atherocalyx, S. beckeana, S. zepcensis (all four belonging to S. recta group), S. alpina subsp. dinarica and S. plumosa. Dry ethanol extracts of aerial flowering plant parts were qualitatively and quantitatively characterized for their phenolic composition using the HPLC-DAD-ESI-MS technique. In total, 44 phenolic compounds were detected, belonging to three main classes of constituents: phenolic acids, phenylethanol glycosides (PhGs) and flavonoids. Chlorogenic acid was the main phenolic acid in all samples. Regarding PhGs, in all investigated extracts acteoside and lavandulifolioside were present. In S. recta group, extracts of S. atherocalyx, S. recta subsp. nitens and S. beckeana showed similar pattern considering phenylethanol glycosides, while S. zepcensis contained only the two aforementioned compounds. PhGs profile of S. plumosa extract comprised the same compounds from S. recta group, but it was also characterized by the presence of few high-molecular compounds. PhGs composition of S. alpina subsp. dinarica extract was somewhat similar with those of S. recta group and also with S. plumosa, but still specific by the presence of echinacoside and leucosceptoside A. The main flavonoids in extracts of Stachys recta group as well as in S. alpina subsp. dinarica extract were 8-hydroxyflavone glycosides (7-O-allosylglucosides of isoscutellarein and hypolaetin and their 4′-methyl derivatives). In addition, in the extract of S. alpina subsp. dinarica two luteolin-7-O-hexosides were also present. On the other hand, S. plumosa extract differed considerably containing mainly derivatives of chrysoeriol and apigenin, while 8-hydroxyflavones (isoscutellarein derivatives) were detected in traces. The results confirmed the importance of flavone 7-O-allosylglucosides at the sectional level of Stachys genus.",
publisher = "Elsevier Ltd",
journal = "Biochemical Systematics and Ecology",
title = "Chemical profiling of six Stachys taxa from Balkan Peninsula",
volume = "104",
doi = "10.1016/j.bse.2022.104482"
}

Karioti, A., Kukić-Marković, J., Bilia, A. R., Niketić, M.,& Petrović, S.. (2022). Chemical profiling of six Stachys taxa from Balkan Peninsula. in Biochemical Systematics and Ecology
Elsevier Ltd., 104.
https://doi.org/10.1016/j.bse.2022.104482

Karioti A, Kukić-Marković J, Bilia AR, Niketić M, Petrović S. Chemical profiling of six Stachys taxa from Balkan Peninsula. in Biochemical Systematics and Ecology. 2022;104.
doi:10.1016/j.bse.2022.104482 .

Karioti, Anastasia, Kukić-Marković, Jelena, Bilia, Anna Rita, Niketić, Marjan, Petrović, Silvana, "Chemical profiling of six Stachys taxa from Balkan Peninsula" in Biochemical Systematics and Ecology, 104 (2022),
https://doi.org/10.1016/j.bse.2022.104482 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4116
AB  - This work is focused on nine Heracleum taxa (Apiaceae): H. sphondylium, H. sibiri- cum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenai- cum subsp. orsinii and H. verticillatum, belonging to the H. sphondylium group, and H. orphanidis, all from sect. Heracleum, as well as H. austriacum subsp. siifolium from sect. Wendia. The aim was to isolate the headspace (HS) fractions from 17 fruit samples (collected from all nine Heracleum taxa) and 13 root samples (col- lected from all eight taxa belonging to sect. Heracleum), to investigate their com- position and chemosystematic significance, as well as to compare these results with those previously obtained for the essential oils isolated by hydrodistillation from the same fruit and root samples. The HS fractions were isolated using an automatic static HS sampler and analysed by GC-FID and GC-MS. The fruit HS fractions were dominated by octyl acetate and/or α-pinene (the taxa from the sect. Heracle- um), or n-octanol (H. austriacum), whereas the root HS fractions mainly contained β-pinene and/or (Z)-β-ocimene (the taxa from the H. sphondylium group), or n-nonane (H. orphanidis). The chemosystematic significance was evaluated using multivariate statistical methods: principal component analysis (PCA), non-metric multidimensional scaling (nMDS) and agglomerative hierarchical clustering based on the unweighted pair-group arithmetic average (UPGMA) algorithm. As in the case of previously investigated essential oils, the statistical analysis of the fruit and root HS fractions generally resulted in the grouping of the investigated H. sphondy- lium group representatives, and within this group, the grouping of morphologically related H. sphondylium and H. montanum and the isolated position of H. verticil- latum. The statistical analysis of the root HS fractions resulted in better separation of the taxa (even compared to previous analysis of the essential oils), i.e. isolated positions of H. ternatum and H. pyrenaicum were observed.
AB  - Fokus ovog rada bio je na devet taksona roda Heracleum (Apiaceae): H. sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii i H. verticillatum iz grupe H. sphondylium, i H. orphanidis, svi iz sect. Heracleum, kao i H. austriacum subsp. siifolium iz sect. Wendia. Cilj rada je bila izolacija headspace (HS) frakcija iz 17 uzoraka plodova (prikupljenih od svih devet ispitivanih taksona roda Heracleum) i 13 uzoraka korena (prikupljenih od svih osam ispitivanih taksona iz sect. Heracleum), ispitivanje njihovog sastava i hemosistematskog značaja, kao i poređenje ovih rezultata sa prethodno dobijenim rezultatima za etarska ulja izolovana destilacijom vodenom parom ovih istih uzoraka plodova i korena. Za izolovanje HS frakcija ko- rišćen je automatski statički HS sampler, a za njihovu analizu gasna hromatografija sa FID i MS detekcijom. U HS frakcijama plodova dominirali su oktilacetat i/ili α-pinen (taksoni iz sect. Heracleum), ili n-oktanol (H. austriacum), a u HS frakcijama korena β-pinen i/ili (Z)-β-ocimen (taksoni iz grupe H. sphondylium), ili n-nonan (H. orphanidis). Hemosistematski značaj je procenjen primenom metoda multivarijantne statističke analize: analize glavnih komponenti (PCA), nemetričkog multidimenzionalnog skaliranja (nMDS) i aglomerativne hijerarhijske klaster analize zasnovane na unweighted pair-group arithmetic averages (UPGMA) algoritmu. Kao i u slučaju prethodno ispitivanih etarskih ulja, u statističkoj analizi HS frakcija plodova i korena generalno je primećeno grupisanje ispi- tivanih predstavnika grupe H. sphondylium, kao i grupisanje morfološki srodnih H. sphondylium i H. montanum i izolovan položaj H. verticillatum u okviru ove grupe. Statistička analiza HS frakcija korena rezultovala je boljim razdvajanjem taksona (čak i u poređenju sa prethodnom analizom etarskih ulja), tj. primećene su izolovane pozicije H. ternatum i H. pyrenaicum.
PB  - Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade
T2  - Botanica Serbica
T1  - Chemical composition and chemosystematic evaluation of the fruit and root
headspace fractions of selected Heracleum taxa from Southeastern Europe
T1  - Hemijski sastav i hemosistematski značaj headspace frakcija plodova i korena
odabranih taksona roda Heracleum iz jugoistočne Evrope
VL  - 46
IS  - 1
SP  - 93
EP  - 103
DO  - 10.2298/BOTSERB2201093U
ER  - 

@article{
author = "Ušjak, Ljuboš and Drobac, Milica and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "This work is focused on nine Heracleum taxa (Apiaceae): H. sphondylium, H. sibiri- cum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenai- cum subsp. orsinii and H. verticillatum, belonging to the H. sphondylium group, and H. orphanidis, all from sect. Heracleum, as well as H. austriacum subsp. siifolium from sect. Wendia. The aim was to isolate the headspace (HS) fractions from 17 fruit samples (collected from all nine Heracleum taxa) and 13 root samples (col- lected from all eight taxa belonging to sect. Heracleum), to investigate their com- position and chemosystematic significance, as well as to compare these results with those previously obtained for the essential oils isolated by hydrodistillation from the same fruit and root samples. The HS fractions were isolated using an automatic static HS sampler and analysed by GC-FID and GC-MS. The fruit HS fractions were dominated by octyl acetate and/or α-pinene (the taxa from the sect. Heracle- um), or n-octanol (H. austriacum), whereas the root HS fractions mainly contained β-pinene and/or (Z)-β-ocimene (the taxa from the H. sphondylium group), or n-nonane (H. orphanidis). The chemosystematic significance was evaluated using multivariate statistical methods: principal component analysis (PCA), non-metric multidimensional scaling (nMDS) and agglomerative hierarchical clustering based on the unweighted pair-group arithmetic average (UPGMA) algorithm. As in the case of previously investigated essential oils, the statistical analysis of the fruit and root HS fractions generally resulted in the grouping of the investigated H. sphondy- lium group representatives, and within this group, the grouping of morphologically related H. sphondylium and H. montanum and the isolated position of H. verticil- latum. The statistical analysis of the root HS fractions resulted in better separation of the taxa (even compared to previous analysis of the essential oils), i.e. isolated positions of H. ternatum and H. pyrenaicum were observed., Fokus ovog rada bio je na devet taksona roda Heracleum (Apiaceae): H. sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii i H. verticillatum iz grupe H. sphondylium, i H. orphanidis, svi iz sect. Heracleum, kao i H. austriacum subsp. siifolium iz sect. Wendia. Cilj rada je bila izolacija headspace (HS) frakcija iz 17 uzoraka plodova (prikupljenih od svih devet ispitivanih taksona roda Heracleum) i 13 uzoraka korena (prikupljenih od svih osam ispitivanih taksona iz sect. Heracleum), ispitivanje njihovog sastava i hemosistematskog značaja, kao i poređenje ovih rezultata sa prethodno dobijenim rezultatima za etarska ulja izolovana destilacijom vodenom parom ovih istih uzoraka plodova i korena. Za izolovanje HS frakcija ko- rišćen je automatski statički HS sampler, a za njihovu analizu gasna hromatografija sa FID i MS detekcijom. U HS frakcijama plodova dominirali su oktilacetat i/ili α-pinen (taksoni iz sect. Heracleum), ili n-oktanol (H. austriacum), a u HS frakcijama korena β-pinen i/ili (Z)-β-ocimen (taksoni iz grupe H. sphondylium), ili n-nonan (H. orphanidis). Hemosistematski značaj je procenjen primenom metoda multivarijantne statističke analize: analize glavnih komponenti (PCA), nemetričkog multidimenzionalnog skaliranja (nMDS) i aglomerativne hijerarhijske klaster analize zasnovane na unweighted pair-group arithmetic averages (UPGMA) algoritmu. Kao i u slučaju prethodno ispitivanih etarskih ulja, u statističkoj analizi HS frakcija plodova i korena generalno je primećeno grupisanje ispi- tivanih predstavnika grupe H. sphondylium, kao i grupisanje morfološki srodnih H. sphondylium i H. montanum i izolovan položaj H. verticillatum u okviru ove grupe. Statistička analiza HS frakcija korena rezultovala je boljim razdvajanjem taksona (čak i u poređenju sa prethodnom analizom etarskih ulja), tj. primećene su izolovane pozicije H. ternatum i H. pyrenaicum.",
publisher = "Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade",
journal = "Botanica Serbica",
title = "Chemical composition and chemosystematic evaluation of the fruit and root
headspace fractions of selected Heracleum taxa from Southeastern Europe, Hemijski sastav i hemosistematski značaj headspace frakcija plodova i korena
odabranih taksona roda Heracleum iz jugoistočne Evrope",
volume = "46",
number = "1",
pages = "93-103",
doi = "10.2298/BOTSERB2201093U"
}

Ušjak, L., Drobac, M., Niketić, M.,& Petrović, S.. (2022). Chemical composition and chemosystematic evaluation of the fruit and root
headspace fractions of selected Heracleum taxa from Southeastern Europe. in Botanica Serbica
Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade., 46(1), 93-103.
https://doi.org/10.2298/BOTSERB2201093U

Ušjak L, Drobac M, Niketić M, Petrović S. Chemical composition and chemosystematic evaluation of the fruit and root
headspace fractions of selected Heracleum taxa from Southeastern Europe. in Botanica Serbica. 2022;46(1):93-103.
doi:10.2298/BOTSERB2201093U .

Ušjak, Ljuboš, Drobac, Milica, Niketić, Marjan, Petrović, Silvana, "Chemical composition and chemosystematic evaluation of the fruit and root
headspace fractions of selected Heracleum taxa from Southeastern Europe" in Botanica Serbica, 46, no. 1 (2022):93-103,
https://doi.org/10.2298/BOTSERB2201093U . .

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Radenković, Mirjana
AU  - Branković, Suzana
AU  - Milenković, Marina
AU  - Niketić, Marjan
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4084
AB  - Dry MeOH extract of Ferula heuffelii (Apiaceae) underground parts was tested for spasmolytic, gastroprotective and antioxidant activities. HPLC analysis revealed that chlorogenic acid (CGA; 34.6 mg/g) was its main constituent. Extract in vitro exhibited notable total antioxidant activity (FRAP value=1.0 μmol Fe2+/mg), and scavenging of DPPH (SC50=62.5 μg/ml) and •OH radicals (49.5 % at 20 μg/ml in 2-deoxyribose assay). In vitro on isolated rat ileum, extract exhibited significant spasmolytic activity, i. e., it showed 124.6 % of maximal atropine effect on spontaneous contractions (at 100 μg/ml), and reduced spasmogenic effect of KCl (80 mm) to 44.4 % (at 60 μg/ml) and of highest applied concentration of ACh to 26.3 % (at 120 μg/ml). In parallel experiments, spasmolytic effect of CGA was also demonstrated. In acute EtOH-induced gastric ulceration model in rats, extract (100 mg/kg p.o.) showed significant gastroprotective effect (gastric damage score 0.50), similar to ranitidine (20 mg/kg p.o.). Obtained results showed that tested F. heuffelii polar extract represents new herbal preparation with potential use against some gastrointestinal complaints.
PB  - John Wiley and Sons Inc
T2  - Chemistry and Biodiversity
T1  - Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts
VL  - 19
IS  - 5
DO  - 10.1002/cbdv.202200047
ER  - 

@article{
author = "Pavlović, Ivan and Radenković, Mirjana and Branković, Suzana and Milenković, Marina and Niketić, Marjan and Ušjak, Ljuboš and Petrović, Silvana",
year = "2022",
abstract = "Dry MeOH extract of Ferula heuffelii (Apiaceae) underground parts was tested for spasmolytic, gastroprotective and antioxidant activities. HPLC analysis revealed that chlorogenic acid (CGA; 34.6 mg/g) was its main constituent. Extract in vitro exhibited notable total antioxidant activity (FRAP value=1.0 μmol Fe2+/mg), and scavenging of DPPH (SC50=62.5 μg/ml) and •OH radicals (49.5 % at 20 μg/ml in 2-deoxyribose assay). In vitro on isolated rat ileum, extract exhibited significant spasmolytic activity, i. e., it showed 124.6 % of maximal atropine effect on spontaneous contractions (at 100 μg/ml), and reduced spasmogenic effect of KCl (80 mm) to 44.4 % (at 60 μg/ml) and of highest applied concentration of ACh to 26.3 % (at 120 μg/ml). In parallel experiments, spasmolytic effect of CGA was also demonstrated. In acute EtOH-induced gastric ulceration model in rats, extract (100 mg/kg p.o.) showed significant gastroprotective effect (gastric damage score 0.50), similar to ranitidine (20 mg/kg p.o.). Obtained results showed that tested F. heuffelii polar extract represents new herbal preparation with potential use against some gastrointestinal complaints.",
publisher = "John Wiley and Sons Inc",
journal = "Chemistry and Biodiversity",
title = "Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts",
volume = "19",
number = "5",
doi = "10.1002/cbdv.202200047"
}

Pavlović, I., Radenković, M., Branković, S., Milenković, M., Niketić, M., Ušjak, L.,& Petrović, S.. (2022). Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts. in Chemistry and Biodiversity
John Wiley and Sons Inc., 19(5).
https://doi.org/10.1002/cbdv.202200047

Pavlović I, Radenković M, Branković S, Milenković M, Niketić M, Ušjak L, Petrović S. Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts. in Chemistry and Biodiversity. 2022;19(5).
doi:10.1002/cbdv.202200047 .

Pavlović, Ivan, Radenković, Mirjana, Branković, Suzana, Milenković, Marina, Niketić, Marjan, Ušjak, Ljuboš, Petrović, Silvana, "Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts" in Chemistry and Biodiversity, 19, no. 5 (2022),
https://doi.org/10.1002/cbdv.202200047 . .

TY  - CONF
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4502
AB  - In this study composition and antimicrobial activity of essential oils of roots, leaves,
stems and fruits of Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae) were investigated.
Plant material was collected in Sićevo Gorge (Kusača) in 2020. Essential oils were isolated by
hydrodistillation in Clevenger-type apparatus and analyzed by GC-FID-MS. Antimicrobial
activity was tested by microdilution method against food contaminants: Gram-positive
(Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes), Gram-negative bacteria
(Escherichia coli, Enterobacter cloacae and Salmonella Typhimurium), and molds (Aspergillus
fumigatus, A. niger, A. versicolor, Penicillium funiculosum, P. verrucosum var. cyclopium and
Trichoderma harzianum); artificial food preservatives E211 and E224 were used as controls.
Dominant constituents of essential oils were monoterpenes (66.2-87.2%). Terpinolene
(36.2%) and p-cymene (11.5%) prevailed in root oil, (E)-β-ocimen (23.2%) and terpinolene
(18.1%) in leaf oil, p-cimen-8-ol (21.8%) and p-cimen (14.1%) in stem oil, and p-cimen
(25.4%) and limonene (14.4%) in fruit oil. Essential oils showed significant antifungal
activity (MIC=0.10-0.78 mg/mL; MFC=0.20-1.56 mg/mL), better compared to both tested
commercial preservatives, and interesting antibacterial activity (MIC=0.20-6.25 mg/mL;
MBC=0.39-12.5 mg/mL), which was in the case of root and stem oils better than activity of
both preservatives against S. aureus and B. cereus. It should be noted that composition of
tested oils was in accordance with composition of the oils of this plant from vicinity of
Madrid (1) and from Crimea (2), while their antimicrobial activity was tested in this work for
the first time. Obtained results justify further research of P. trifida essential oils as new
natural food preservatives.
AB  - Cilj ovog rada bio je da se ispitaju hemijski sastav i antimikrobna aktivnost etarskih
ulja korena, listova, stabljika i plodova Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae).
Biljni materijal je prikupljen na području Sićevačke klisure (Kusača) 2020. god. Etarska ulja
su dobijena destilacijom vodenom parom u aparaturi po Klevendžeru i analizirana metodom
GC-FID-MS. Antimikrobna aktivnost testirana je mikrodilucionom metodom na
kontaminantima hrane: Gram-pozitivnim (Staphylococcus aureus, Bacillus cereus i Listeria
monocytogenes), Gram-negativnim bakterijama (Escherichia coli, Enterobacter cloacae i
Salmonella Typhimurium), i plesnima (Aspergillus fumigatus, A. niger, A. versicolor,
Penicillium funiculosum, P. verrucosum var. cyclopium i Trichoderma harzianum); kao
kontrole korišćeni su sintetski konzervansi E211 i E224. Dominantni sastojci etarskih ulja
bili su monoterpeni (66,2-87,2%). Najzastupljeniji u ulju korena bili su terpinolen (36,2%) i
p-cimen (11,5%), u ulju listova (E)-β-ocimen (23,2%) i terpinolen (18,1%), u ulju stabljika p-cimen-
8-ol (21,8%) i p-cimen (14,1%), a u ulju plodova p-cimen (25,4%) i limonen (14,4%).
Etarska ulja su pokazala značajnu antifungalnu aktivnost (MIK=0,10-0,78 mg/mL;
MFK=0,20-1,56 mg/mL), bolju u poređenju sa oba testirana komercijalna konzervansa, i
interesantnu antibakterijsku aktivnost (MIK=0,20-6,25 mg/mL; MBK=0,39-12,5 mg/mL),
koja je u slučaju ulja korena i stabljika prema S. aureus i B. cereus bila bolja u odnosu na oba
konzervansa. Treba istaći da je sastav ispitivanih etarskih ulja bio u skladu sa sastavom ulja
ove biljke iz okoline Madrida (1) i sa Krima (2), dok je njihova antimikrobna aktivnost
ispitivana u ovom radu po prvi put. Dobijeni rezultati predstavljaju dobru osnovu za dalja
istraživanja etarskih ulja P. trifida kao novih prirodnih konzervanasa.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l.
T1  - Hemijski sastav i antimikrobna aktivnost etarskih ulja Prangos trifida s.l.
VL  - 72
IS  - 4 suplement
SP  - 455
EP  - 456
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4502
ER  - 

@conference{
author = "Ušjak, Ljuboš and Stojković, Dejan and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "In this study composition and antimicrobial activity of essential oils of roots, leaves,
stems and fruits of Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae) were investigated.
Plant material was collected in Sićevo Gorge (Kusača) in 2020. Essential oils were isolated by
hydrodistillation in Clevenger-type apparatus and analyzed by GC-FID-MS. Antimicrobial
activity was tested by microdilution method against food contaminants: Gram-positive
(Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes), Gram-negative bacteria
(Escherichia coli, Enterobacter cloacae and Salmonella Typhimurium), and molds (Aspergillus
fumigatus, A. niger, A. versicolor, Penicillium funiculosum, P. verrucosum var. cyclopium and
Trichoderma harzianum); artificial food preservatives E211 and E224 were used as controls.
Dominant constituents of essential oils were monoterpenes (66.2-87.2%). Terpinolene
(36.2%) and p-cymene (11.5%) prevailed in root oil, (E)-β-ocimen (23.2%) and terpinolene
(18.1%) in leaf oil, p-cimen-8-ol (21.8%) and p-cimen (14.1%) in stem oil, and p-cimen
(25.4%) and limonene (14.4%) in fruit oil. Essential oils showed significant antifungal
activity (MIC=0.10-0.78 mg/mL; MFC=0.20-1.56 mg/mL), better compared to both tested
commercial preservatives, and interesting antibacterial activity (MIC=0.20-6.25 mg/mL;
MBC=0.39-12.5 mg/mL), which was in the case of root and stem oils better than activity of
both preservatives against S. aureus and B. cereus. It should be noted that composition of
tested oils was in accordance with composition of the oils of this plant from vicinity of
Madrid (1) and from Crimea (2), while their antimicrobial activity was tested in this work for
the first time. Obtained results justify further research of P. trifida essential oils as new
natural food preservatives., Cilj ovog rada bio je da se ispitaju hemijski sastav i antimikrobna aktivnost etarskih
ulja korena, listova, stabljika i plodova Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae).
Biljni materijal je prikupljen na području Sićevačke klisure (Kusača) 2020. god. Etarska ulja
su dobijena destilacijom vodenom parom u aparaturi po Klevendžeru i analizirana metodom
GC-FID-MS. Antimikrobna aktivnost testirana je mikrodilucionom metodom na
kontaminantima hrane: Gram-pozitivnim (Staphylococcus aureus, Bacillus cereus i Listeria
monocytogenes), Gram-negativnim bakterijama (Escherichia coli, Enterobacter cloacae i
Salmonella Typhimurium), i plesnima (Aspergillus fumigatus, A. niger, A. versicolor,
Penicillium funiculosum, P. verrucosum var. cyclopium i Trichoderma harzianum); kao
kontrole korišćeni su sintetski konzervansi E211 i E224. Dominantni sastojci etarskih ulja
bili su monoterpeni (66,2-87,2%). Najzastupljeniji u ulju korena bili su terpinolen (36,2%) i
p-cimen (11,5%), u ulju listova (E)-β-ocimen (23,2%) i terpinolen (18,1%), u ulju stabljika p-cimen-
8-ol (21,8%) i p-cimen (14,1%), a u ulju plodova p-cimen (25,4%) i limonen (14,4%).
Etarska ulja su pokazala značajnu antifungalnu aktivnost (MIK=0,10-0,78 mg/mL;
MFK=0,20-1,56 mg/mL), bolju u poređenju sa oba testirana komercijalna konzervansa, i
interesantnu antibakterijsku aktivnost (MIK=0,20-6,25 mg/mL; MBK=0,39-12,5 mg/mL),
koja je u slučaju ulja korena i stabljika prema S. aureus i B. cereus bila bolja u odnosu na oba
konzervansa. Treba istaći da je sastav ispitivanih etarskih ulja bio u skladu sa sastavom ulja
ove biljke iz okoline Madrida (1) i sa Krima (2), dok je njihova antimikrobna aktivnost
ispitivana u ovom radu po prvi put. Dobijeni rezultati predstavljaju dobru osnovu za dalja
istraživanja etarskih ulja P. trifida kao novih prirodnih konzervanasa.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l., Hemijski sastav i antimikrobna aktivnost etarskih ulja Prangos trifida s.l.",
volume = "72",
number = "4 suplement",
pages = "455-456",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4502"
}

Ušjak, L., Stojković, D., Soković, M., Niketić, M.,& Petrović, S.. (2022). Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l.. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), 455-456.
https://hdl.handle.net/21.15107/rcub_farfar_4502

Ušjak L, Stojković D, Soković M, Niketić M, Petrović S. Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l.. in Arhiv za farmaciju. 2022;72(4 suplement):455-456.
https://hdl.handle.net/21.15107/rcub_farfar_4502 .

Ušjak, Ljuboš, Stojković, Dejan, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l." in Arhiv za farmaciju, 72, no. 4 suplement (2022):455-456,
https://hdl.handle.net/21.15107/rcub_farfar_4502 .

TY  - CONF
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4503
AB  - In this work, composition and antioxidant activity of dry dichloromethane and methanol extracts of Heracleum ternatum Velen. (Apiaceae) leaves and flowers from Mt Durmitor (Montenegro) were investigated. Using LC-MS, five furanocoumarins were identified in leaf dichloromethane extract (accounting for 23.00 mg/g of dry extract) and eight in flower dichloromethane extract (60.47 mg/g). Dominant in leaf extract was heraclenin (11.64 mg/g), followed by imperatorin (5.90 mg/g), and in flower extract heraclenol (17.55 mg/g), followed by heraclenin (15.04 mg/g). LC-MS analysis revealed eight flavonol glycosides in leaf methanol extract (accounting for 16.18 mg/g of dry extract) and 11 in flower methanol extract (65.82 mg/g), as well as chlorogenic acid in both extracts (4.72 mg/g and trace, respectively). Among detected flavonoids, dominant was quercetin 7-O-rhamnosyl 3-O-glucoside (vincetoxicoside A; 7.95 and 16.77 mg/g), followed by biosides and triosides of kaempferol and methylquercetin. Content of total polyphenols, determined spectrophotometrically using Folin-Ciocalteu reagent, was 87.29 and 98.12 mg of gallic acid equivalents/g of dry methanol extracts. Among methanol extracts, polyphenol richer flower extract showed higher, spectrophotometrically determined, total antioxidant and anti-DPPH activities compared to leaf extract (FRAP=0.80 and 0.65 mmol Fe2+/g; DPPH SC50=83.12 and 90.95 μg/mL). Dichloromethane extracts showed lower anti-DPPH activity (SC50=860.58 and 515.76 μg/mL).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia, Belgrade
C3  - 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
T1  - Heracleum ternatum from Mt. Durmitor: furanocoumarins, polyphenols and antioxidant activity of leaf and flower extracts
SP  - 143
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4503
ER  - 

@conference{
author = "Ušjak, Ljuboš and Drobac, Milica and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "In this work, composition and antioxidant activity of dry dichloromethane and methanol extracts of Heracleum ternatum Velen. (Apiaceae) leaves and flowers from Mt Durmitor (Montenegro) were investigated. Using LC-MS, five furanocoumarins were identified in leaf dichloromethane extract (accounting for 23.00 mg/g of dry extract) and eight in flower dichloromethane extract (60.47 mg/g). Dominant in leaf extract was heraclenin (11.64 mg/g), followed by imperatorin (5.90 mg/g), and in flower extract heraclenol (17.55 mg/g), followed by heraclenin (15.04 mg/g). LC-MS analysis revealed eight flavonol glycosides in leaf methanol extract (accounting for 16.18 mg/g of dry extract) and 11 in flower methanol extract (65.82 mg/g), as well as chlorogenic acid in both extracts (4.72 mg/g and trace, respectively). Among detected flavonoids, dominant was quercetin 7-O-rhamnosyl 3-O-glucoside (vincetoxicoside A; 7.95 and 16.77 mg/g), followed by biosides and triosides of kaempferol and methylquercetin. Content of total polyphenols, determined spectrophotometrically using Folin-Ciocalteu reagent, was 87.29 and 98.12 mg of gallic acid equivalents/g of dry methanol extracts. Among methanol extracts, polyphenol richer flower extract showed higher, spectrophotometrically determined, total antioxidant and anti-DPPH activities compared to leaf extract (FRAP=0.80 and 0.65 mmol Fe2+/g; DPPH SC50=83.12 and 90.95 μg/mL). Dichloromethane extracts showed lower anti-DPPH activity (SC50=860.58 and 515.76 μg/mL).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia, Belgrade",
journal = "14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts",
title = "Heracleum ternatum from Mt. Durmitor: furanocoumarins, polyphenols and antioxidant activity of leaf and flower extracts",
pages = "143-143",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4503"
}

Ušjak, L., Drobac, M., Niketić, M.,& Petrović, S.. (2022). Heracleum ternatum from Mt. Durmitor: furanocoumarins, polyphenols and antioxidant activity of leaf and flower extracts. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 143-143.
https://hdl.handle.net/21.15107/rcub_farfar_4503

Ušjak L, Drobac M, Niketić M, Petrović S. Heracleum ternatum from Mt. Durmitor: furanocoumarins, polyphenols and antioxidant activity of leaf and flower extracts. in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts. 2022;:143-143.
https://hdl.handle.net/21.15107/rcub_farfar_4503 .

Ušjak, Ljuboš, Drobac, Milica, Niketić, Marjan, Petrović, Silvana, "Heracleum ternatum from Mt. Durmitor: furanocoumarins, polyphenols and antioxidant activity of leaf and flower extracts" in 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions - Book of Abstracts (2022):143-143,
https://hdl.handle.net/21.15107/rcub_farfar_4503 .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Milutinović, Violeta
AU  - Đorđić Crnogorac, Marija J.
AU  - Stanojković, Tatjana P.
AU  - Niketić, Marjan S.
AU  - Kukić-Marković, Jelena
AU  - Petrović, Silvana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3957
AB  - Dry MeOH extracts of the twig barks of Pyrus communis subsp. pyraster, P. spinosa and their hybrid P.×jordanovii nothosubsp. velenovskyi, collected in wild in Serbia, were analyzed. By LC/MS, the contents of arbutin (99.9–131.0 mg/g), chlorogenic acid (2.2–6.3 mg/g), catechin (1.0–5.3 mg/g) and total dimeric and trimeric procyanidins (42.2–61.3 mg/g), including procyanidin B2 (8.9–17.2 mg/g), were determined. Colorimetri- cally, high contents of total phenolics (436.2–533.4 mg GAE/g) and tannins (339.4–425.7 mg GAE/g), as well as strong total antioxidant activities (FRAP values 4.5–5.9 mmol Fe2+ /g), and DPPH (SC50 = 6.6–7.1 μg/ml) and hydroxyl radical (SC50 = 447.1–727.7 μg/ml) scavenging abilities were revealed. In vitro, all extracts exhibited notable inhibition of α-amylase (IC50 = 310.8–617.7 μg/ml) and particularly strong inhibition of α-glucosidase (IC50 = 2.1–3.7 μg/ml). Molecular docking predicted that among identified compounds procyanidin B2 is the best inhibitor of these carbohydrate-digesting enzymes. Obtained results showed that the barks of investigated Pyrus hybrid and its parent taxa have similar composition and bioactivity.
PB  - John Wiley and Sons Inc
T2  - Chemistry & Biodiversity
T1  - Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition
VL  - 18
IS  - 10
DO  - 10.1002/cbdv.202100446
ER  - 

@article{
author = "Ušjak, Ljuboš and Milutinović, Violeta and Đorđić Crnogorac, Marija J. and Stanojković, Tatjana P. and Niketić, Marjan S. and Kukić-Marković, Jelena and Petrović, Silvana",
year = "2021",
abstract = "Dry MeOH extracts of the twig barks of Pyrus communis subsp. pyraster, P. spinosa and their hybrid P.×jordanovii nothosubsp. velenovskyi, collected in wild in Serbia, were analyzed. By LC/MS, the contents of arbutin (99.9–131.0 mg/g), chlorogenic acid (2.2–6.3 mg/g), catechin (1.0–5.3 mg/g) and total dimeric and trimeric procyanidins (42.2–61.3 mg/g), including procyanidin B2 (8.9–17.2 mg/g), were determined. Colorimetri- cally, high contents of total phenolics (436.2–533.4 mg GAE/g) and tannins (339.4–425.7 mg GAE/g), as well as strong total antioxidant activities (FRAP values 4.5–5.9 mmol Fe2+ /g), and DPPH (SC50 = 6.6–7.1 μg/ml) and hydroxyl radical (SC50 = 447.1–727.7 μg/ml) scavenging abilities were revealed. In vitro, all extracts exhibited notable inhibition of α-amylase (IC50 = 310.8–617.7 μg/ml) and particularly strong inhibition of α-glucosidase (IC50 = 2.1–3.7 μg/ml). Molecular docking predicted that among identified compounds procyanidin B2 is the best inhibitor of these carbohydrate-digesting enzymes. Obtained results showed that the barks of investigated Pyrus hybrid and its parent taxa have similar composition and bioactivity.",
publisher = "John Wiley and Sons Inc",
journal = "Chemistry & Biodiversity",
title = "Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition",
volume = "18",
number = "10",
doi = "10.1002/cbdv.202100446"
}

Ušjak, L., Milutinović, V., Đorđić Crnogorac, M. J., Stanojković, T. P., Niketić, M. S., Kukić-Marković, J.,& Petrović, S.. (2021). Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition. in Chemistry & Biodiversity
John Wiley and Sons Inc., 18(10).
https://doi.org/10.1002/cbdv.202100446

Ušjak L, Milutinović V, Đorđić Crnogorac MJ, Stanojković TP, Niketić MS, Kukić-Marković J, Petrović S. Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition. in Chemistry & Biodiversity. 2021;18(10).
doi:10.1002/cbdv.202100446 .

Ušjak, Ljuboš, Milutinović, Violeta, Đorđić Crnogorac, Marija J., Stanojković, Tatjana P., Niketić, Marjan S., Kukić-Marković, Jelena, Petrović, Silvana, "Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition" in Chemistry & Biodiversity, 18, no. 10 (2021),
https://doi.org/10.1002/cbdv.202100446 . .