TY  - JOUR
AU  - Račić, Anđelka
AU  - Jurišić-Dukovski, Bisera
AU  - Lovrić, Jasmina
AU  - Dobričić, Vladimir
AU  - Vučen, Sonja
AU  - Micov, Ana
AU  - Stepanović-Petrović, Radica
AU  - Tomić, Maja
AU  - Pecikoza, Uroš
AU  - Bajac, Jelena
AU  - Krajišnik, Danina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5610
AB  - The incorporation of polymers into drug delivery vehicles has been shown to be a useful approach to prolong the
residence time of drugs in the precorneal tear film and to improve penetration into biological membranes. The
main objective of this research was to formulate novel viscous eye drops with ketotifen as the active ingredient,
containing the polysaccharides: chitosan (MCH), hydroxypropyl guar gum (HPG) and hyaluronic acid (SH) alone
and in combination as functional polymers. DSC and FT-IR techniques showed the compatibility between
ketotifen and polymers. Physicochemical and rheological analysis at ambient and simulated physiological conditions, as well as the evaluation of mucoadhesive properties showed that vehicles containing combinations of
polymers have suitable physicochemical and functional properties with demonstrated synergism between
combined polymers (MCH and HPG i.e. SH and HPG). The drug permeability was successfully estimated in vitro
using HCE-T cell-based models. MTT cytotoxicity assay demonstrates that the tested formulations were non-toxic
and well tolerated. In vivo preclinical study on mice revealed that both vehicles containing mixed polymers
enhanced and prolonged the antipruritic/analgesic-like effect of ophthalmic ketotifen. Based on these results,
both combinations of polysaccharide polymers, especially SH-HPG, could be considered as potential new carriers
for ketotifen for ophthalmic use.
PB  - Elsevier
T2  - International Journal of Pharmaceutics
T1  - Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy
VL  - 655
SP  - 124033
DO  - 10.1016/j.ijpharm.2024.124033
ER  - 

@article{
author = "Račić, Anđelka and Jurišić-Dukovski, Bisera and Lovrić, Jasmina and Dobričić, Vladimir and Vučen, Sonja and Micov, Ana and Stepanović-Petrović, Radica and Tomić, Maja and Pecikoza, Uroš and Bajac, Jelena and Krajišnik, Danina",
year = "2024",
abstract = "The incorporation of polymers into drug delivery vehicles has been shown to be a useful approach to prolong the
residence time of drugs in the precorneal tear film and to improve penetration into biological membranes. The
main objective of this research was to formulate novel viscous eye drops with ketotifen as the active ingredient,
containing the polysaccharides: chitosan (MCH), hydroxypropyl guar gum (HPG) and hyaluronic acid (SH) alone
and in combination as functional polymers. DSC and FT-IR techniques showed the compatibility between
ketotifen and polymers. Physicochemical and rheological analysis at ambient and simulated physiological conditions, as well as the evaluation of mucoadhesive properties showed that vehicles containing combinations of
polymers have suitable physicochemical and functional properties with demonstrated synergism between
combined polymers (MCH and HPG i.e. SH and HPG). The drug permeability was successfully estimated in vitro
using HCE-T cell-based models. MTT cytotoxicity assay demonstrates that the tested formulations were non-toxic
and well tolerated. In vivo preclinical study on mice revealed that both vehicles containing mixed polymers
enhanced and prolonged the antipruritic/analgesic-like effect of ophthalmic ketotifen. Based on these results,
both combinations of polysaccharide polymers, especially SH-HPG, could be considered as potential new carriers
for ketotifen for ophthalmic use.",
publisher = "Elsevier",
journal = "International Journal of Pharmaceutics",
title = "Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy",
volume = "655",
pages = "124033",
doi = "10.1016/j.ijpharm.2024.124033"
}

Račić, A., Jurišić-Dukovski, B., Lovrić, J., Dobričić, V., Vučen, S., Micov, A., Stepanović-Petrović, R., Tomić, M., Pecikoza, U., Bajac, J.,& Krajišnik, D.. (2024). Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy. in International Journal of Pharmaceutics
Elsevier., 655, 124033.
https://doi.org/10.1016/j.ijpharm.2024.124033

Račić A, Jurišić-Dukovski B, Lovrić J, Dobričić V, Vučen S, Micov A, Stepanović-Petrović R, Tomić M, Pecikoza U, Bajac J, Krajišnik D. Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy. in International Journal of Pharmaceutics. 2024;655:124033.
doi:10.1016/j.ijpharm.2024.124033 .

Račić, Anđelka, Jurišić-Dukovski, Bisera, Lovrić, Jasmina, Dobričić, Vladimir, Vučen, Sonja, Micov, Ana, Stepanović-Petrović, Radica, Tomić, Maja, Pecikoza, Uroš, Bajac, Jelena, Krajišnik, Danina, "Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy" in International Journal of Pharmaceutics, 655 (2024):124033,
https://doi.org/10.1016/j.ijpharm.2024.124033 . .

TY  - CONF
AU  - Bubić Pajić, Nataša
AU  - Ilić, Tanja
AU  - Nikolić, Ines
AU  - Vučen, Sonja
AU  - Dobričić, Vladimir
AU  - Savić, Snežana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5455
AB  - Efikasnost terapije gljivičnih infekcija kože zavisi od sposobnosti lijeka da premosti barijeru stratum
corneuma i dospije u vijabilni epidermis. S obzirom da su mnogi antifungalni lijekovi slabo rastvorljivi
u vodi i imaju nisku permeabilnost, razvoj pogodnih formulacija za efikasnu isporuku lijeka u kožu
nije jednostavan.
Cilj ovog rada je procjena efekta kombinovane primjene prethodno razvijenih biokompatibilnih
mikroemulzija i čvrstih silikonskih mikroigala na poboljšanu dermalnu isporuku antifungalnog lijeka
- sertakonazol-nitrata (SN).
Mikroemulzije su izrađivane dodatkom ulja (propilenglikol-monokaprilat) u smješu surfaktanta
i kosurfaktanta (gliceret-7-kaprilat/kaprat:etanol ili polisorbat 80: dietilenglikol monoetiletar)
uz miješanje u trajanju od 15 min. Nakon toga, odgovarajuća količina vode je postepeno dodana
i miješanje je vršeno dodatnih 15 min, nakon čega je u izrađenim uzorcima rastvoren SN (2%). In
vitro studije penetracije/permeacije sa ili bez predthodnog tretmana kože uha svinje ImmuPatch
silikonskim mikroiglama (Nacionalni Institut Tyndall, Kork, Irska) su sprovedene na Franz-ovim
difuzionim ćelijama.
Dobijeni rezultati su pokazali da je predtrtman kože mikroiglama značajno poboljšao isporuku SN
u koži iz obje ispitivane mikroemulzione formulacije. 24h nakon primjene uzoraka, količina lijeka
koja je deponovana u koži tretiranoj mikroiglama je bila veća 1,90-2,02 puta u odnosu na intaktnu
kožu. Međutim, količina lijeka koja je permeirala nakon 24h iz mikroemulzija kroz kožu tretiranu
mikroiglama je bila 26,96±7,88 μg/ml i 12,66±2,07 μg/ml, dok SN nije detektovan u akceptorskoj fazi
nakon primjene samih mikroemulzija.
Dobijeni rezultati ukazuju da bi kombinovana primjena mikroigala i biokompatibilnih mikroemulzija
mogla biti efikasan i bezbolan pristup za poboljšanu isporuku sertakonazol-nitrata u kožu. Međutim,
bez obzira na zanemarljivu permeaciju, odgovarajućim in vivo ispitivanjima na je potrebno potvrditi
odsustvo sistemskih neželjenih efekata.
AB  - The effectiveness of a topically applied antifungal agent is directly related to its ability to pass the barrier of the stratum corneum and to reach at least the viable epidermis. Given that many antifungals are poorly soluble and have low skin permeability, development of a suitable formulation is not an easy task. The aim of our study was to evaluate the effect of the combination of previously designed seraconazole nitrate (SN) loaded biocompatible microemulsions with solid silicon microneedles for enhanced dermal drug delivery. For the preparation of MEs, oil (propylene glycol monocaprylate) was added to the mixture of surfactant and cosurfactant (glycereth-7-caprylate/caprate:ethanol or polysorbate 80: diethylene glycolmonoethyl ether) and mixed for 15 min. Then, proper amount of water was added and mixed for another 15 min. SN (2% w/w) was dissolved in previously prepared MEs. In vitro skin penetration/ permeation studies with and without skin pretreatment with ImmuPatch silicon microneedles (Tyndall National Institute, Cork, Ireland) were conducted on Franz-type diffusion cells using porcine ear skin. Our finding revealed that skin pretreatment with microneedles significantly improved SN delivery into the skin from both tested microemulsions, resulting in 1.90-2.02-fold enhanced level of the drug retained in the skin, as measured 24h after administration. However, the drug permeated 24h after application of tested formulations combined with microneedles was 26.96±7.88 μg/ml and 12.66±2.07 μg/ml, whereas SN did not permeated through the intact skin. The obtained results suggest that combination of solid microneedles and biocompatible microemulsions could be an effective and painless approach for enhancing drug delivery into the skin. However, despite negligible SN permeation, further in vivo study should be performed in order to confirm the absence of systemic adverse effects.
PB  - Prisma - korporativne komunikacije
PB  - Farmaceutska komora Crne Gore
C3  - Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09 - 12. maj 2019. Budva, Bečići, Book of Abstracts
T1  - Kombinovana primjena silikonskih mikroigala i biokompatibilnih mikroemulzija za poboljšanu dermalnu isporuku sertakonazol-nitrata
T1  - Combined application of silicon microneedles and biocompatible microemulsions for improved dermal delivery of sertaconazole nitrate
SP  - 118
EP  - 119
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5455
ER  - 

@conference{
author = "Bubić Pajić, Nataša and Ilić, Tanja and Nikolić, Ines and Vučen, Sonja and Dobričić, Vladimir and Savić, Snežana",
year = "2019",
abstract = "Efikasnost terapije gljivičnih infekcija kože zavisi od sposobnosti lijeka da premosti barijeru stratum
corneuma i dospije u vijabilni epidermis. S obzirom da su mnogi antifungalni lijekovi slabo rastvorljivi
u vodi i imaju nisku permeabilnost, razvoj pogodnih formulacija za efikasnu isporuku lijeka u kožu
nije jednostavan.
Cilj ovog rada je procjena efekta kombinovane primjene prethodno razvijenih biokompatibilnih
mikroemulzija i čvrstih silikonskih mikroigala na poboljšanu dermalnu isporuku antifungalnog lijeka
- sertakonazol-nitrata (SN).
Mikroemulzije su izrađivane dodatkom ulja (propilenglikol-monokaprilat) u smješu surfaktanta
i kosurfaktanta (gliceret-7-kaprilat/kaprat:etanol ili polisorbat 80: dietilenglikol monoetiletar)
uz miješanje u trajanju od 15 min. Nakon toga, odgovarajuća količina vode je postepeno dodana
i miješanje je vršeno dodatnih 15 min, nakon čega je u izrađenim uzorcima rastvoren SN (2%). In
vitro studije penetracije/permeacije sa ili bez predthodnog tretmana kože uha svinje ImmuPatch
silikonskim mikroiglama (Nacionalni Institut Tyndall, Kork, Irska) su sprovedene na Franz-ovim
difuzionim ćelijama.
Dobijeni rezultati su pokazali da je predtrtman kože mikroiglama značajno poboljšao isporuku SN
u koži iz obje ispitivane mikroemulzione formulacije. 24h nakon primjene uzoraka, količina lijeka
koja je deponovana u koži tretiranoj mikroiglama je bila veća 1,90-2,02 puta u odnosu na intaktnu
kožu. Međutim, količina lijeka koja je permeirala nakon 24h iz mikroemulzija kroz kožu tretiranu
mikroiglama je bila 26,96±7,88 μg/ml i 12,66±2,07 μg/ml, dok SN nije detektovan u akceptorskoj fazi
nakon primjene samih mikroemulzija.
Dobijeni rezultati ukazuju da bi kombinovana primjena mikroigala i biokompatibilnih mikroemulzija
mogla biti efikasan i bezbolan pristup za poboljšanu isporuku sertakonazol-nitrata u kožu. Međutim,
bez obzira na zanemarljivu permeaciju, odgovarajućim in vivo ispitivanjima na je potrebno potvrditi
odsustvo sistemskih neželjenih efekata., The effectiveness of a topically applied antifungal agent is directly related to its ability to pass the barrier of the stratum corneum and to reach at least the viable epidermis. Given that many antifungals are poorly soluble and have low skin permeability, development of a suitable formulation is not an easy task. The aim of our study was to evaluate the effect of the combination of previously designed seraconazole nitrate (SN) loaded biocompatible microemulsions with solid silicon microneedles for enhanced dermal drug delivery. For the preparation of MEs, oil (propylene glycol monocaprylate) was added to the mixture of surfactant and cosurfactant (glycereth-7-caprylate/caprate:ethanol or polysorbate 80: diethylene glycolmonoethyl ether) and mixed for 15 min. Then, proper amount of water was added and mixed for another 15 min. SN (2% w/w) was dissolved in previously prepared MEs. In vitro skin penetration/ permeation studies with and without skin pretreatment with ImmuPatch silicon microneedles (Tyndall National Institute, Cork, Ireland) were conducted on Franz-type diffusion cells using porcine ear skin. Our finding revealed that skin pretreatment with microneedles significantly improved SN delivery into the skin from both tested microemulsions, resulting in 1.90-2.02-fold enhanced level of the drug retained in the skin, as measured 24h after administration. However, the drug permeated 24h after application of tested formulations combined with microneedles was 26.96±7.88 μg/ml and 12.66±2.07 μg/ml, whereas SN did not permeated through the intact skin. The obtained results suggest that combination of solid microneedles and biocompatible microemulsions could be an effective and painless approach for enhancing drug delivery into the skin. However, despite negligible SN permeation, further in vivo study should be performed in order to confirm the absence of systemic adverse effects.",
publisher = "Prisma - korporativne komunikacije, Farmaceutska komora Crne Gore",
journal = "Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09 - 12. maj 2019. Budva, Bečići, Book of Abstracts",
title = "Kombinovana primjena silikonskih mikroigala i biokompatibilnih mikroemulzija za poboljšanu dermalnu isporuku sertakonazol-nitrata, Combined application of silicon microneedles and biocompatible microemulsions for improved dermal delivery of sertaconazole nitrate",
pages = "118-119",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5455"
}

Bubić Pajić, N., Ilić, T., Nikolić, I., Vučen, S., Dobričić, V.,& Savić, S.. (2019). Kombinovana primjena silikonskih mikroigala i biokompatibilnih mikroemulzija za poboljšanu dermalnu isporuku sertakonazol-nitrata. in Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09 - 12. maj 2019. Budva, Bečići, Book of Abstracts
Prisma - korporativne komunikacije., 118-119.
https://hdl.handle.net/21.15107/rcub_farfar_5455

Bubić Pajić N, Ilić T, Nikolić I, Vučen S, Dobričić V, Savić S. Kombinovana primjena silikonskih mikroigala i biokompatibilnih mikroemulzija za poboljšanu dermalnu isporuku sertakonazol-nitrata. in Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09 - 12. maj 2019. Budva, Bečići, Book of Abstracts. 2019;:118-119.
https://hdl.handle.net/21.15107/rcub_farfar_5455 .

Bubić Pajić, Nataša, Ilić, Tanja, Nikolić, Ines, Vučen, Sonja, Dobričić, Vladimir, Savić, Snežana, "Kombinovana primjena silikonskih mikroigala i biokompatibilnih mikroemulzija za poboljšanu dermalnu isporuku sertakonazol-nitrata" in Treći kongres farmaceuta Crne Gore sa međunarodnim učešćem, 09 - 12. maj 2019. Budva, Bečići, Book of Abstracts (2019):118-119,
https://hdl.handle.net/21.15107/rcub_farfar_5455 .

TY  - JOUR
AU  - Bubić-Pajić, Nataša
AU  - Todosijević, Marija N.
AU  - Vuleta, Gordana
AU  - Cekić, Nebojša
AU  - Dobričić, Vladimir
AU  - Vučen, Sonja
AU  - Čalija, Bojan
AU  - Lukić, Milica
AU  - Ilić, Tanja
AU  - Savić, Snežana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2837
AB  - Two types of biocompatible surfactants were evaluated for their capability to formulate skin-friendly/non-irritant microemulsions as vehicles for two poorly water-soluble model drugs differing in properties and concentrations: alkyl polyglucosides (decyl glucoside and caprylyl/capryl glucoside) and ethoxylated surfactants (glycereth-7-caprylate/caprate and polysorbate 80). Phase behavior, structural inversion and microemulsion solubilization potential for sertaconazole nitrate and adapalene were found to be highly dependent on the surfactants structure and HLB value. Performed characterization (polarized light microscopy, pH, electrical conductivity, rheological, FTIR and DSC measurements) indicated a formulation containing glycereth-7-caprylate/caprate as suitable for incorporation of both drugs, whereas alkyl polyglucoside-based systems did not exhibit satisfying solubilization capacity for sertaconazole nitrate. Further, monitored parameters were strongly affected by sertaconazole nitrate incorporation, while they remained almost unchanged in adapalene-loaded vehicles. In addition, results of the in vivo skin performance study supported acceptable tolerability for all investigated formulations, suggesting selected microemulsions as promising carriers worth exploring further for effective skin delivery of model drugs.
PB  - Hrvatsko Farmaceutsko Drustov (HFD)-Croation Pharmaceutical Soc, Zagreb
T2  - Acta Pharmaceutica
T1  - Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance
VL  - 67
IS  - 4
SP  - 415
EP  - 439
DO  - 10.1515/acph-2017-0036
ER  - 

@article{
author = "Bubić-Pajić, Nataša and Todosijević, Marija N. and Vuleta, Gordana and Cekić, Nebojša and Dobričić, Vladimir and Vučen, Sonja and Čalija, Bojan and Lukić, Milica and Ilić, Tanja and Savić, Snežana",
year = "2017",
abstract = "Two types of biocompatible surfactants were evaluated for their capability to formulate skin-friendly/non-irritant microemulsions as vehicles for two poorly water-soluble model drugs differing in properties and concentrations: alkyl polyglucosides (decyl glucoside and caprylyl/capryl glucoside) and ethoxylated surfactants (glycereth-7-caprylate/caprate and polysorbate 80). Phase behavior, structural inversion and microemulsion solubilization potential for sertaconazole nitrate and adapalene were found to be highly dependent on the surfactants structure and HLB value. Performed characterization (polarized light microscopy, pH, electrical conductivity, rheological, FTIR and DSC measurements) indicated a formulation containing glycereth-7-caprylate/caprate as suitable for incorporation of both drugs, whereas alkyl polyglucoside-based systems did not exhibit satisfying solubilization capacity for sertaconazole nitrate. Further, monitored parameters were strongly affected by sertaconazole nitrate incorporation, while they remained almost unchanged in adapalene-loaded vehicles. In addition, results of the in vivo skin performance study supported acceptable tolerability for all investigated formulations, suggesting selected microemulsions as promising carriers worth exploring further for effective skin delivery of model drugs.",
publisher = "Hrvatsko Farmaceutsko Drustov (HFD)-Croation Pharmaceutical Soc, Zagreb",
journal = "Acta Pharmaceutica",
title = "Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance",
volume = "67",
number = "4",
pages = "415-439",
doi = "10.1515/acph-2017-0036"
}

Bubić-Pajić, N., Todosijević, M. N., Vuleta, G., Cekić, N., Dobričić, V., Vučen, S., Čalija, B., Lukić, M., Ilić, T.,& Savić, S.. (2017). Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance. in Acta Pharmaceutica
Hrvatsko Farmaceutsko Drustov (HFD)-Croation Pharmaceutical Soc, Zagreb., 67(4), 415-439.
https://doi.org/10.1515/acph-2017-0036

Bubić-Pajić N, Todosijević MN, Vuleta G, Cekić N, Dobričić V, Vučen S, Čalija B, Lukić M, Ilić T, Savić S. Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance. in Acta Pharmaceutica. 2017;67(4):415-439.
doi:10.1515/acph-2017-0036 .

Bubić-Pajić, Nataša, Todosijević, Marija N., Vuleta, Gordana, Cekić, Nebojša, Dobričić, Vladimir, Vučen, Sonja, Čalija, Bojan, Lukić, Milica, Ilić, Tanja, Savić, Snežana, "Alkyl polyglucoside vs. ethoxylated surfactant-based microemulsions as vehicles for two poorly water-soluble drugs: physicochemical characterization and in vivo skin performance" in Acta Pharmaceutica, 67, no. 4 (2017):415-439,
https://doi.org/10.1515/acph-2017-0036 . .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Savić, Snežana
AU  - Krajišnik, Danina
AU  - Daniels, Rolf
AU  - Vučen, Sonja
AU  - Marković, Bojan
AU  - Milić, Jela
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2429
AB  - The objectives of this study were to prepare films from submicron chitosan/Eudragit((R)) L100-55 polyelectrolyte complexes (CH/EL PEC) and to assess the influence of CH molecular weight and CH/EL mass ratio on their structure and drug-release properties. The films were obtained by a simple, environmentally friendly, casting/solvent evaporation method and the verapamil hydrochloride (VH) was used as model drug. Submicron size, narrow size distribution, and acceptable stability of CH/EL PECs were confirmed by DLS and laser Doppler microelectrophoresis. SEM analysis revealed nonporous inner structure and flat surface of the films. Interactions between comprising polymers and formation of CH/EL PEC were established by DSC and FT-IR spectroscopy. In vitro swelling and drug release studies revealed the pH sensitivity of the films, with burst drug release in acidic conditions (pH 1.2) and sustained release in phosphate buffers pH 5.8, 6.8, and 7.4. The slowest VH release was achieved from the films prepared from equal amounts of EL and CH of higher molecular weight, confirming the significance of the CH/EL ratio and CH molecular weight on their ability to sustain drug release. The obtained results suggested that presented, simple, and eco-friendly preparation procedure can be used to obtain pH-sensitive CH/EL PEC films with a promising potential as drug carriers.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Applied Polymer Science
T1  - pH-sensitive polyelectrolyte films derived from submicron chitosan/Eudragit((R)) L 100-55 complexes: Physicochemical characterization and in vitro drug release
VL  - 132
IS  - 39
DO  - 10.1002/app.42583
ER  - 

@article{
author = "Čalija, Bojan and Savić, Snežana and Krajišnik, Danina and Daniels, Rolf and Vučen, Sonja and Marković, Bojan and Milić, Jela",
year = "2015",
abstract = "The objectives of this study were to prepare films from submicron chitosan/Eudragit((R)) L100-55 polyelectrolyte complexes (CH/EL PEC) and to assess the influence of CH molecular weight and CH/EL mass ratio on their structure and drug-release properties. The films were obtained by a simple, environmentally friendly, casting/solvent evaporation method and the verapamil hydrochloride (VH) was used as model drug. Submicron size, narrow size distribution, and acceptable stability of CH/EL PECs were confirmed by DLS and laser Doppler microelectrophoresis. SEM analysis revealed nonporous inner structure and flat surface of the films. Interactions between comprising polymers and formation of CH/EL PEC were established by DSC and FT-IR spectroscopy. In vitro swelling and drug release studies revealed the pH sensitivity of the films, with burst drug release in acidic conditions (pH 1.2) and sustained release in phosphate buffers pH 5.8, 6.8, and 7.4. The slowest VH release was achieved from the films prepared from equal amounts of EL and CH of higher molecular weight, confirming the significance of the CH/EL ratio and CH molecular weight on their ability to sustain drug release. The obtained results suggested that presented, simple, and eco-friendly preparation procedure can be used to obtain pH-sensitive CH/EL PEC films with a promising potential as drug carriers.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Applied Polymer Science",
title = "pH-sensitive polyelectrolyte films derived from submicron chitosan/Eudragit((R)) L 100-55 complexes: Physicochemical characterization and in vitro drug release",
volume = "132",
number = "39",
doi = "10.1002/app.42583"
}

Čalija, B., Savić, S., Krajišnik, D., Daniels, R., Vučen, S., Marković, B.,& Milić, J.. (2015). pH-sensitive polyelectrolyte films derived from submicron chitosan/Eudragit((R)) L 100-55 complexes: Physicochemical characterization and in vitro drug release. in Journal of Applied Polymer Science
Wiley-Blackwell, Hoboken., 132(39).
https://doi.org/10.1002/app.42583

Čalija B, Savić S, Krajišnik D, Daniels R, Vučen S, Marković B, Milić J. pH-sensitive polyelectrolyte films derived from submicron chitosan/Eudragit((R)) L 100-55 complexes: Physicochemical characterization and in vitro drug release. in Journal of Applied Polymer Science. 2015;132(39).
doi:10.1002/app.42583 .

Čalija, Bojan, Savić, Snežana, Krajišnik, Danina, Daniels, Rolf, Vučen, Sonja, Marković, Bojan, Milić, Jela, "pH-sensitive polyelectrolyte films derived from submicron chitosan/Eudragit((R)) L 100-55 complexes: Physicochemical characterization and in vitro drug release" in Journal of Applied Polymer Science, 132, no. 39 (2015),
https://doi.org/10.1002/app.42583 . .

TY  - JOUR
AU  - Vučen, Sonja
AU  - Bubić-Pajić, Nataša
AU  - Savić, Snežana
AU  - Vuleta, Gordana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2273
AB  - The efficiency of(trans)dermal drug delivery has been severely reduced by the inability of most drugs to penetrate the skin at therapeutically useful rates. Recently, the use of micron-scale needles has been shown to increase skin permeability and significantly improve (trans)dermal drug delivery, particularly for macromolecules. Using the tools of the microelectronics industry and different materials, microneedles of various sizes and shapes have been fabricated. Due to their micron size (less than 300 μm in diameter and 50-900 μm in length), these needles do not reach the nerve endings in the dermis, providing a painless application, which is considered as their main advantage over hypodermic needles. Pathways created using microneedles are orders of magnitude bigger than molecular dimensions and, therefore, should readily permit transport of macromolecules, as well as possibly supramolecular complexes and microparticles Recently, in vivo studies have demonstrated a successful delivery of oligonucleotides, insulin, and many other drugs using a microneedles, as well as induction of the immune response from protein and DNA vaccines. Numerous studies conducted in this research field have led to launching the first microneedle-based (trans)dermal drug delivery systems on the market.
AB  - Efikasnost terapijskih sistema za (trans)dermalnu isporuku lijekova je u znatnoj mjeri umanjena zbog otežanog prolaska lijekova u/kroz kožu. Nedavno je pokazano da je upotrebom igala mikronskih veličina moguće povećati permeabilnost kože i znatno poboljšati (trans)dermalnu isporuku, naročito u slučaju makromolekula. Primjenom dostignuća mikroelektronske industrije, od različitih materijala su proizvedene mikroigle različitih veličina i oblika. S obzirom na svoje mikronske veličine (prečnika obično oko 1 μm i dužine 50-900 μm), ovakve igle ne dopiru do nervnih završetaka u dermisu, čime obezbjeđuju bezbolnu primjenu, što se smatra njihovom osnovnom prednošću u odnosu na potkožne igle. Nastale pore u koži su nekoliko puta veće od dimenzija molekula, te je omogućen transport makromolekula, kao i supramolekularnih kompleksa i nano/mikročestica. Dosadašnje in vivo studije su pokazale uspješnu (trans)dermalnu isporuku oligonukleotida, insulina i drugih lijekova primjenom mikroigala, kao i indukciju imunskog odgovora proteinskim i DNK vakcinama. Brojni radovi sprovedeni u ovoj oblasti istraživanja doveli su i do pojave na tržištu prvih sistema za (trans)dermalnu isporuku lijekova zasnovanih na tehnologiji mikroigala.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Microneedles: Physical enhancers for (trans)dermal drug delivery
T1  - Mikroigle - fizički pojačivači (trans)dermalne isporuke lijekova
VL  - 64
IS  - 4
SP  - 295
EP  - 321
DO  - 10.5937/arhfarm1404295V
ER  - 

@article{
author = "Vučen, Sonja and Bubić-Pajić, Nataša and Savić, Snežana and Vuleta, Gordana",
year = "2014",
abstract = "The efficiency of(trans)dermal drug delivery has been severely reduced by the inability of most drugs to penetrate the skin at therapeutically useful rates. Recently, the use of micron-scale needles has been shown to increase skin permeability and significantly improve (trans)dermal drug delivery, particularly for macromolecules. Using the tools of the microelectronics industry and different materials, microneedles of various sizes and shapes have been fabricated. Due to their micron size (less than 300 μm in diameter and 50-900 μm in length), these needles do not reach the nerve endings in the dermis, providing a painless application, which is considered as their main advantage over hypodermic needles. Pathways created using microneedles are orders of magnitude bigger than molecular dimensions and, therefore, should readily permit transport of macromolecules, as well as possibly supramolecular complexes and microparticles Recently, in vivo studies have demonstrated a successful delivery of oligonucleotides, insulin, and many other drugs using a microneedles, as well as induction of the immune response from protein and DNA vaccines. Numerous studies conducted in this research field have led to launching the first microneedle-based (trans)dermal drug delivery systems on the market., Efikasnost terapijskih sistema za (trans)dermalnu isporuku lijekova je u znatnoj mjeri umanjena zbog otežanog prolaska lijekova u/kroz kožu. Nedavno je pokazano da je upotrebom igala mikronskih veličina moguće povećati permeabilnost kože i znatno poboljšati (trans)dermalnu isporuku, naročito u slučaju makromolekula. Primjenom dostignuća mikroelektronske industrije, od različitih materijala su proizvedene mikroigle različitih veličina i oblika. S obzirom na svoje mikronske veličine (prečnika obično oko 1 μm i dužine 50-900 μm), ovakve igle ne dopiru do nervnih završetaka u dermisu, čime obezbjeđuju bezbolnu primjenu, što se smatra njihovom osnovnom prednošću u odnosu na potkožne igle. Nastale pore u koži su nekoliko puta veće od dimenzija molekula, te je omogućen transport makromolekula, kao i supramolekularnih kompleksa i nano/mikročestica. Dosadašnje in vivo studije su pokazale uspješnu (trans)dermalnu isporuku oligonukleotida, insulina i drugih lijekova primjenom mikroigala, kao i indukciju imunskog odgovora proteinskim i DNK vakcinama. Brojni radovi sprovedeni u ovoj oblasti istraživanja doveli su i do pojave na tržištu prvih sistema za (trans)dermalnu isporuku lijekova zasnovanih na tehnologiji mikroigala.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Microneedles: Physical enhancers for (trans)dermal drug delivery, Mikroigle - fizički pojačivači (trans)dermalne isporuke lijekova",
volume = "64",
number = "4",
pages = "295-321",
doi = "10.5937/arhfarm1404295V"
}

Vučen, S., Bubić-Pajić, N., Savić, S.,& Vuleta, G.. (2014). Microneedles: Physical enhancers for (trans)dermal drug delivery. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 64(4), 295-321.
https://doi.org/10.5937/arhfarm1404295V

Vučen S, Bubić-Pajić N, Savić S, Vuleta G. Microneedles: Physical enhancers for (trans)dermal drug delivery. in Arhiv za farmaciju. 2014;64(4):295-321.
doi:10.5937/arhfarm1404295V .

Vučen, Sonja, Bubić-Pajić, Nataša, Savić, Snežana, Vuleta, Gordana, "Microneedles: Physical enhancers for (trans)dermal drug delivery" in Arhiv za farmaciju, 64, no. 4 (2014):295-321,
https://doi.org/10.5937/arhfarm1404295V . .