TY  - CONF
AU  - Jauković, Valentina
AU  - Krajišnik, Danina
AU  - Čalija, Bojan
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4565
AB  - Halloysite is a clay mineral which can be used as a nanocontainer for prolonged
release of active substances, due to its biocompatibility, tubular structure, positive lumen
and negative outer surface charge. However, the use of halloysite is limited by low loading
capacity and mucoadhesiveness (1). To increase pore volume and prolong residence time of
a drug, halloysite was treated with acetic acid and functionalized with mucoadhesive
polymers, chitosan (a cationic polysaccharide) (2) and methacrylated chitosan (degree of
methacrylation 27%). The aim of this study was to evaluate the impact of chitosan
methacrylation on mucoadhesiveness of halloysite-chitosan nanocomposites. Binding of both
polymers at the halloysite surface was confirmed by zeta potential measurements. Halloysite
and halloysite-polymer nanocomposites were mixed with an aqueous porcine mucin
dispersion (0.1% m/m). After incubation with mucin during 8 h at room temperature, zeta
potentials of halloysite-chitosan (HCN) and halloysite-methacrylated chitosan
nanocomposites (HMCN) decreased, from +25.9 to -13.3 mV i.e. from +24.4 to -12.6 mV,
respectively. The measured zeta potentials were close to zeta potential for pure mucin (-12.2
mV), indicating their interaction. Mucoadhesive properties were further investigated by
measuring ability of HCN and HMCN to adsorb mucin using UV/VIS spectroscopy. HMCN
were able to adsorb higher % of mucin (≈82%) compared to HCN (≈72%) and pristine
halloysite (≈ 58%) after 8 h of incubation. Modification of halloysite with methacrylated
chitosan has shown to be efficient in improving of mucoadhesiveness of halloysite-chitosan
nanocomposites, which makes them prospective materials for drug delivery applications.
AB  - Halojzit je mineral iz grupe glina koji se može koristiti kao nanonosač za produženo
oslobađanje aktivne supstance, zahvaljujući svojoj biokompatibilnosti, tubularnoj strukturi,
pozitivno naelektrisanoj unutrašnjoj površini i negativno naelektrisanoj spoljašnjoj površini.
Međutim, primjenu halojzita ograničavaju nizak kapacitet za inkapsulaciju i
mukoadhezivnost (1). Kako bi se povećala zapremina pora i produžilo vrijeme zadržavanja
aktivne supstance, halojzit je tretiran sirćetnom kisjelinom i površinski funkcionalizovan
mukoadhezivnim polimerima, hitozanom (katjonski polisaharid) (2) i metakrilovanim
hitozanom (stepen metakrilacije 27%). Cilj ovog rada bio je da se ispita uticaj metakrilacije
hitozana na mukoadhezivnost halojzit-hitozan nanokompozita. Određivanjem zeta
potencijala potvrđeno je vezivanje oba polimera za površinu halojzita. Halojzit i halojzit-
polimer nanokompoziti pomiješani su sa vodenom disperzijom svinjskog mucina (0,1%
m/m). Nakon inkubacije sa mucinom tokom 8 h na sobnoj temperaturi, došlo je do smanjenja
vrijednosti zeta potencijala halojzit-hitozan (HCN) i halojzit-metakrilovani hitozan
nanokompozita (HMCN), od +25,9 do -13,3 mV tj. od +24,4 do -12,6 mV, redom. Dobijene
vrijednosti zeta potencijala su bile približne zeta potencijalu čistog mucina (-12,2 mV), što
ukazuje na njihovu međusobnu interakciju. Mukoadhezivne osobine su dodatno ispitane
određivanjem sposobnosti HCN i HMCN da adsorbuju mucin, upotrebom UV/VIS
spektroskopije. HMCN je adsorbovao veći % mucina (≈82%) u odnosu na HCN (≈72%) i
polazni halojzit (≈ 58%) nakon 8 h inkubacije. Modifikacija halojzita metakrilovanim
hitozanom pokazala se efikasnom metodom za poboljšanje mukoadhezivnosti halojzit-
hitozan nanokompozita, što ih čini potencijalnim materijalima za isporuku aktivnih
supstanci.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites
T1  - Uticaj metakrilacije hitozana na mukoadhezivnost halojzit‐ hitozan nanokompozita kao nosača za produženo oslobađanje aktivne supstance
VL  - 72
IS  - 4 suplement
SP  - S384
EP  - S385
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4565
ER  - 

@conference{
author = "Jauković, Valentina and Krajišnik, Danina and Čalija, Bojan",
year = "2022",
abstract = "Halloysite is a clay mineral which can be used as a nanocontainer for prolonged
release of active substances, due to its biocompatibility, tubular structure, positive lumen
and negative outer surface charge. However, the use of halloysite is limited by low loading
capacity and mucoadhesiveness (1). To increase pore volume and prolong residence time of
a drug, halloysite was treated with acetic acid and functionalized with mucoadhesive
polymers, chitosan (a cationic polysaccharide) (2) and methacrylated chitosan (degree of
methacrylation 27%). The aim of this study was to evaluate the impact of chitosan
methacrylation on mucoadhesiveness of halloysite-chitosan nanocomposites. Binding of both
polymers at the halloysite surface was confirmed by zeta potential measurements. Halloysite
and halloysite-polymer nanocomposites were mixed with an aqueous porcine mucin
dispersion (0.1% m/m). After incubation with mucin during 8 h at room temperature, zeta
potentials of halloysite-chitosan (HCN) and halloysite-methacrylated chitosan
nanocomposites (HMCN) decreased, from +25.9 to -13.3 mV i.e. from +24.4 to -12.6 mV,
respectively. The measured zeta potentials were close to zeta potential for pure mucin (-12.2
mV), indicating their interaction. Mucoadhesive properties were further investigated by
measuring ability of HCN and HMCN to adsorb mucin using UV/VIS spectroscopy. HMCN
were able to adsorb higher % of mucin (≈82%) compared to HCN (≈72%) and pristine
halloysite (≈ 58%) after 8 h of incubation. Modification of halloysite with methacrylated
chitosan has shown to be efficient in improving of mucoadhesiveness of halloysite-chitosan
nanocomposites, which makes them prospective materials for drug delivery applications., Halojzit je mineral iz grupe glina koji se može koristiti kao nanonosač za produženo
oslobađanje aktivne supstance, zahvaljujući svojoj biokompatibilnosti, tubularnoj strukturi,
pozitivno naelektrisanoj unutrašnjoj površini i negativno naelektrisanoj spoljašnjoj površini.
Međutim, primjenu halojzita ograničavaju nizak kapacitet za inkapsulaciju i
mukoadhezivnost (1). Kako bi se povećala zapremina pora i produžilo vrijeme zadržavanja
aktivne supstance, halojzit je tretiran sirćetnom kisjelinom i površinski funkcionalizovan
mukoadhezivnim polimerima, hitozanom (katjonski polisaharid) (2) i metakrilovanim
hitozanom (stepen metakrilacije 27%). Cilj ovog rada bio je da se ispita uticaj metakrilacije
hitozana na mukoadhezivnost halojzit-hitozan nanokompozita. Određivanjem zeta
potencijala potvrđeno je vezivanje oba polimera za površinu halojzita. Halojzit i halojzit-
polimer nanokompoziti pomiješani su sa vodenom disperzijom svinjskog mucina (0,1%
m/m). Nakon inkubacije sa mucinom tokom 8 h na sobnoj temperaturi, došlo je do smanjenja
vrijednosti zeta potencijala halojzit-hitozan (HCN) i halojzit-metakrilovani hitozan
nanokompozita (HMCN), od +25,9 do -13,3 mV tj. od +24,4 do -12,6 mV, redom. Dobijene
vrijednosti zeta potencijala su bile približne zeta potencijalu čistog mucina (-12,2 mV), što
ukazuje na njihovu međusobnu interakciju. Mukoadhezivne osobine su dodatno ispitane
određivanjem sposobnosti HCN i HMCN da adsorbuju mucin, upotrebom UV/VIS
spektroskopije. HMCN je adsorbovao veći % mucina (≈82%) u odnosu na HCN (≈72%) i
polazni halojzit (≈ 58%) nakon 8 h inkubacije. Modifikacija halojzita metakrilovanim
hitozanom pokazala se efikasnom metodom za poboljšanje mukoadhezivnosti halojzit-
hitozan nanokompozita, što ih čini potencijalnim materijalima za isporuku aktivnih
supstanci.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites, Uticaj metakrilacije hitozana na mukoadhezivnost halojzit‐ hitozan nanokompozita kao nosača za produženo oslobađanje aktivne supstance",
volume = "72",
number = "4 suplement",
pages = "S384-S385",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4565"
}

Jauković, V., Krajišnik, D.,& Čalija, B.. (2022). Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S384-S385.
https://hdl.handle.net/21.15107/rcub_farfar_4565

Jauković V, Krajišnik D, Čalija B. Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites. in Arhiv za farmaciju. 2022;72(4 suplement):S384-S385.
https://hdl.handle.net/21.15107/rcub_farfar_4565 .

Jauković, Valentina, Krajišnik, Danina, Čalija, Bojan, "Influence of chitosan methacrylation on mucoadhesiveness of halloysite-chitosan sustained release nanocomposites" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S384-S385,
https://hdl.handle.net/21.15107/rcub_farfar_4565 .

TY  - JOUR
AU  - Jauković, Valentina
AU  - Krajišnik, Danina
AU  - Daković, Aleksandra
AU  - Damjanović, Ana
AU  - Krstić, Jugoslav
AU  - Stojanović, Jovica
AU  - Čalija, Bojan
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3796
AB  - The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.
PB  - Elsevier Ltd
T2  - Materials Science and Engineering C
T1  - Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release
VL  - 123
DO  - 10.1016/j.msec.2021.112029
ER  - 

@article{
author = "Jauković, Valentina and Krajišnik, Danina and Daković, Aleksandra and Damjanović, Ana and Krstić, Jugoslav and Stojanović, Jovica and Čalija, Bojan",
year = "2021",
abstract = "The functionality of halloysite (Hal) nanotubes as drug carriers can be improved by lumen enlargement and polymer modification. This study investigates the influence of selective acid etching on Hal functionalization with cationic biopolymer chitosan. Hal was subjected to lumen etching under mild conditions, loaded under vacuum with nonsteroidal antiinflammatory drug aceclofenac, and incubated in an acidic solution of chitosan. The functionality of pristine and etched Hal before and upon polymer functionalization was assessed by ζ-potential measurements, structural characterization (FT-IR, DSC and XRPD analysis), cell viability assay, drug loading and drug release studies. Acid etching increased specific surface area, pore volume and pore size of Hal, decreased ζ-potential and facilitated binding of the cationic polymer. XRPD and DSC analysis revealed crystalline structure of etched Hal. Successful chitosan binding and drug entrapment were further confirmed by FT-IR and DSC studies. XRPD showed surface polymer binding. DSC and FT-IR analyses confirmed the presence of the entrapped drug in its crystalline form. Drug loading was increased for ≈81% by selective lumen etching. Slight decrease of drug content occurred during chitosan functionalization due to aceclofenac diffusion in the polymer solution. The drug release was more sustained from etched Hal nanocomposites (up to ≈87% for 12 h) than from pristine Hal (up to ≈97% for 12 h) due to more intensive chitosan binding. High human fibroblast survival rates upon exposure to pristine and etched Hal before and after chitosan functionalization (>90% in the concentration of 1000 μg/mL) confirmed that both lumen etching under mild conditions and polymer functionalization had no significant effect on cytocompatibility. Based on these findings, selective lumen etching in combination with polycation modification appears to be a promising approach for improvement of Hal nanotubes functionality by increasing payload, polymer binding capacity, and sustained release properties with no significant effect on their cytocompatibility.",
publisher = "Elsevier Ltd",
journal = "Materials Science and Engineering C",
title = "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release",
volume = "123",
doi = "10.1016/j.msec.2021.112029"
}

Jauković, V., Krajišnik, D., Daković, A., Damjanović, A., Krstić, J., Stojanović, J.,& Čalija, B.. (2021). Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C
Elsevier Ltd., 123.
https://doi.org/10.1016/j.msec.2021.112029

Jauković V, Krajišnik D, Daković A, Damjanović A, Krstić J, Stojanović J, Čalija B. Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release. in Materials Science and Engineering C. 2021;123.
doi:10.1016/j.msec.2021.112029 .

Jauković, Valentina, Krajišnik, Danina, Daković, Aleksandra, Damjanović, Ana, Krstić, Jugoslav, Stojanović, Jovica, Čalija, Bojan, "Influence of selective acid-etching on functionality of halloysite-chitosan nanocontainers for sustained drug release" in Materials Science and Engineering C, 123 (2021),
https://doi.org/10.1016/j.msec.2021.112029 . .