TY  - JOUR
AU  - Ušjak, Dušan
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3752
AB  - An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.
PB  - Wiley-VCH Verlag
T2  - Chemistry and Biodiversity
T1  - Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,
Adhesion and Surface Motility of Acinetobacter baumannii by
Inhibiting ompA Gene Expression
VL  - 18
IS  - 1
DO  - 10.1002/cbdv.202000786
ER  - 

@article{
author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2021",
abstract = "An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.",
publisher = "Wiley-VCH Verlag",
journal = "Chemistry and Biodiversity",
title = "Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,
Adhesion and Surface Motility of Acinetobacter baumannii by
Inhibiting ompA Gene Expression",
volume = "18",
number = "1",
doi = "10.1002/cbdv.202000786"
}

Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M.. (2021). Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,
Adhesion and Surface Motility of Acinetobacter baumannii by
Inhibiting ompA Gene Expression. in Chemistry and Biodiversity
Wiley-VCH Verlag., 18(1).
https://doi.org/10.1002/cbdv.202000786

Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković M. Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,
Adhesion and Surface Motility of Acinetobacter baumannii by
Inhibiting ompA Gene Expression. in Chemistry and Biodiversity. 2021;18(1).
doi:10.1002/cbdv.202000786 .

Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,
Adhesion and Surface Motility of Acinetobacter baumannii by
Inhibiting ompA Gene Expression" in Chemistry and Biodiversity, 18, no. 1 (2021),
https://doi.org/10.1002/cbdv.202000786 . .

TY  - CONF
AU  - Ušjak, Dušan
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3758
AB  - Uvod: Acinetobacter baumannii je globalno rasprostranjen nozokomijalni patogen koji se odlikuje izuzetnom sposobnošću ekstremno brzog sticanja rezistencije na antibiotike, kao i adaptacije na preživljavanje u suvim uslovima bolničke sredine [1]. Zbog velike zastupljenosti rezistentnih sojeva protiv kojih ne postoji delotvorna terapija, Svetska zdravstvena organizacija (WHO, 2017) i Centri za kontrolu i prevenciju bolesti (CDC, 2019), označili su A. baumannii kao patogen od kritične važnosti za otkriće novih antimikrobnih agenasa ili novih terapijskih strategija [2]. Targetiranje virulencije je oblik alternativnog terapijskog pristupa koji pruža mogućnost prevencije teže kliničke slike kod inficiranih pacijenata posredstvom inhibicije ekspresije ključnih faktora virulencije, uz istovremenu redukovanu selekciju rezistentnih mutanata [3].
Rezultati i Diskusija: Od četiri različito supstituisana hidroksihalkona, sintetisanih u postupku bazno-katalizovane Claisen-Schmidt kondenzacije, selektiran je metkosi-supstituisani derivat kao najpotentniji inhibitor produkcije biofilma kod A. baumannii. Primenom Real-Time kvantitativne PCR metode sa reverznom transkriptazom ispitan je uticaj subinhibitornih koncentracija selektiranog jedinjenja (70, 35 i 10 μg/mL) na ekspresiju gena faktora virulencije povezanih sa produkcijom biofilma kod A. baumannii: ompA, bap i abaI. Pokazana je značajna dozno-zavisna nishodna ekspresija ompA gena, koji kodira OmpA protein spoljašnje membrane ćelijskog zida, koji učestvuje u brojnim virulentnim osobinama A. baumannii, kao što su adhezija, citotoksičnost, motilitet i rezistencija na imunski odgovor i antibiotike [4]. Takođe, zabeležena je značajna inhibicija ekspresije bap gena, koja je neophodna za adheziju na humane epitelne ćelije, i abaI gena, integralnog dela bakterijskog kvorum-sensing sistema, koji kodira sintazu autoinduktorskih molekula. Sposobnost antivirulentnog delovanja metoksi-supstituisanog derivata hidroksihalkona potvrđena je demonstracijom inhibicije fenotipske ekspresije faktora virulencije povezanih sa ekspresijom ompA, bap i abaI gena, kao što su adhezija za komponente ekstracelularnog matriksa (fibronektin i kolagen), površinski motilitet i produkcija autoinduktorskih molekula.
Zaključak: Metoksi-supstituisani hidroksihalkon ispoljava antivirulentno dejstvo protiv A. baumannii, pre svega posredstvom nishodne regulacije ompA gena, što se reflektuje u inhibiciji produkcije biofilma, sposobnosti adhezije i površinskog motiliteta ovog patogena.
AB  - Over the last two decades, Acinetobacter baumannii has emerged as one of the most troublesome pathogens, rapidly acquiring resistance to virtually all available antibiotics. This has urged researchers to seek alternative ways to fight this pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm activity of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. We used quantitative Real-Time PCR to evaluate mRNA expression of virulence-associated genes (ompA, bap, abaI) in extensively drug-resistant (XDR) A. baumannii wound isolate and A. baumannii ATCC 19606 strain, treated with selected compound. Also, we tested biofilm production, fibronectin- and collagen-mediated adhesion, surface motility and quorum-sensing activity of treated strains. The results revealed downregulation of the expression of all tested virulence genes together with the reduction of biofilm production, adhesion and motility. The most notable finding is significant reduction of ompA gene expression, whose encoded protein product is associated with numerous virulence traits of A. baumannii. Therefore, we conclude that selected methoxy-substituted hydroxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of the bacterial adhesins, most importantly OmpA, which is reflected in reduced biofilm formation, adhesion and surface motility.
C3  - FEMS Online Conference on Microbiology
T1  - Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3758
ER  - 

@conference{
author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2020",
abstract = "Uvod: Acinetobacter baumannii je globalno rasprostranjen nozokomijalni patogen koji se odlikuje izuzetnom sposobnošću ekstremno brzog sticanja rezistencije na antibiotike, kao i adaptacije na preživljavanje u suvim uslovima bolničke sredine [1]. Zbog velike zastupljenosti rezistentnih sojeva protiv kojih ne postoji delotvorna terapija, Svetska zdravstvena organizacija (WHO, 2017) i Centri za kontrolu i prevenciju bolesti (CDC, 2019), označili su A. baumannii kao patogen od kritične važnosti za otkriće novih antimikrobnih agenasa ili novih terapijskih strategija [2]. Targetiranje virulencije je oblik alternativnog terapijskog pristupa koji pruža mogućnost prevencije teže kliničke slike kod inficiranih pacijenata posredstvom inhibicije ekspresije ključnih faktora virulencije, uz istovremenu redukovanu selekciju rezistentnih mutanata [3].
Rezultati i Diskusija: Od četiri različito supstituisana hidroksihalkona, sintetisanih u postupku bazno-katalizovane Claisen-Schmidt kondenzacije, selektiran je metkosi-supstituisani derivat kao najpotentniji inhibitor produkcije biofilma kod A. baumannii. Primenom Real-Time kvantitativne PCR metode sa reverznom transkriptazom ispitan je uticaj subinhibitornih koncentracija selektiranog jedinjenja (70, 35 i 10 μg/mL) na ekspresiju gena faktora virulencije povezanih sa produkcijom biofilma kod A. baumannii: ompA, bap i abaI. Pokazana je značajna dozno-zavisna nishodna ekspresija ompA gena, koji kodira OmpA protein spoljašnje membrane ćelijskog zida, koji učestvuje u brojnim virulentnim osobinama A. baumannii, kao što su adhezija, citotoksičnost, motilitet i rezistencija na imunski odgovor i antibiotike [4]. Takođe, zabeležena je značajna inhibicija ekspresije bap gena, koja je neophodna za adheziju na humane epitelne ćelije, i abaI gena, integralnog dela bakterijskog kvorum-sensing sistema, koji kodira sintazu autoinduktorskih molekula. Sposobnost antivirulentnog delovanja metoksi-supstituisanog derivata hidroksihalkona potvrđena je demonstracijom inhibicije fenotipske ekspresije faktora virulencije povezanih sa ekspresijom ompA, bap i abaI gena, kao što su adhezija za komponente ekstracelularnog matriksa (fibronektin i kolagen), površinski motilitet i produkcija autoinduktorskih molekula.
Zaključak: Metoksi-supstituisani hidroksihalkon ispoljava antivirulentno dejstvo protiv A. baumannii, pre svega posredstvom nishodne regulacije ompA gena, što se reflektuje u inhibiciji produkcije biofilma, sposobnosti adhezije i površinskog motiliteta ovog patogena., Over the last two decades, Acinetobacter baumannii has emerged as one of the most troublesome pathogens, rapidly acquiring resistance to virtually all available antibiotics. This has urged researchers to seek alternative ways to fight this pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm activity of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. We used quantitative Real-Time PCR to evaluate mRNA expression of virulence-associated genes (ompA, bap, abaI) in extensively drug-resistant (XDR) A. baumannii wound isolate and A. baumannii ATCC 19606 strain, treated with selected compound. Also, we tested biofilm production, fibronectin- and collagen-mediated adhesion, surface motility and quorum-sensing activity of treated strains. The results revealed downregulation of the expression of all tested virulence genes together with the reduction of biofilm production, adhesion and motility. The most notable finding is significant reduction of ompA gene expression, whose encoded protein product is associated with numerous virulence traits of A. baumannii. Therefore, we conclude that selected methoxy-substituted hydroxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of the bacterial adhesins, most importantly OmpA, which is reflected in reduced biofilm formation, adhesion and surface motility.",
journal = "FEMS Online Conference on Microbiology",
title = "Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3758"
}

Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M.. (2020). Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression. in FEMS Online Conference on Microbiology.
https://hdl.handle.net/21.15107/rcub_farfar_3758

Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković M. Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression. in FEMS Online Conference on Microbiology. 2020;.
https://hdl.handle.net/21.15107/rcub_farfar_3758 .

Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression" in FEMS Online Conference on Microbiology (2020),
https://hdl.handle.net/21.15107/rcub_farfar_3758 .

TY  - JOUR
AU  - Miljković, Marija
AU  - Jovanović, Sofija
AU  - O'Connor, Paula M.
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Dinić, Miroslav
AU  - Studholme, David John
AU  - Fira, Đorđe
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3372
AB  - Bacteria active against multi-drug resistant pathogens, isolated by direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative (Klebsiella pneumoniae Ni9 and Pseudomonas aeruginosa MMA83) and two Gram-positive (Staphylococcus aureus ATCC25923 and Listeria monocytogenes ATCC19111) pathogens. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 produced the largest zones of inhibition against all four pathogens when grown in LB broth with aeration at 37 degrees C. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 were identified as Brevibacillus laterosporus and pulsed field gel electrophoresis and extracellular protein profiles showed that three different strains (BGSP7, BGSP9 and BGSP11) were isolated. A semi-native SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) gel overlay assay showed that BGSP7 and BGSP9 produce small antimicrobial molecules of about 1.5 kDa, while BGSP11 produces antimicrobial molecules of 1.5 and 6 kDa active against S. aureus ATCC25923. Amino acid analysis of two antimicrobial molecules (1583.73 Da; from BGSP7 and 1556.31 Da; from BGSP11) revealed that they have a similar composition and differ only by virtue of the presence of a methionine which is present only in BGSP11 molecule. Genome sequencing of the three isolates revealed the presence of gene clusters associated with the production of non-ribosomally synthesized peptides (brevibacillin, bogorol, gramicidin S, plipastatin and tyrocin) and bacteriocins (laterosporulin, a lactococcin 972-like bacteriocin, as well as putative linocin M18, sactipeptide, UviB and lantipeptide-like molecules). Ultimately, the purification of a number of antimicrobial molecules from each isolate suggests that they can be considered as potent biocontrol strains that produce an arsenal of antimicrobial molecules active against Gram-positive and Gram-negative multi-resistant pathogens, fungi and insects.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials
VL  - 14
IS  - 5
DO  - 10.1371/journal.pone.0216773
ER  - 

@article{
author = "Miljković, Marija and Jovanović, Sofija and O'Connor, Paula M. and Mirković, Nemanja and Jovčić, Branko and Filipić, Brankica and Dinić, Miroslav and Studholme, David John and Fira, Đorđe and Cotter, Paul D. and Kojić, Milan",
year = "2019",
abstract = "Bacteria active against multi-drug resistant pathogens, isolated by direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative (Klebsiella pneumoniae Ni9 and Pseudomonas aeruginosa MMA83) and two Gram-positive (Staphylococcus aureus ATCC25923 and Listeria monocytogenes ATCC19111) pathogens. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 produced the largest zones of inhibition against all four pathogens when grown in LB broth with aeration at 37 degrees C. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 were identified as Brevibacillus laterosporus and pulsed field gel electrophoresis and extracellular protein profiles showed that three different strains (BGSP7, BGSP9 and BGSP11) were isolated. A semi-native SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) gel overlay assay showed that BGSP7 and BGSP9 produce small antimicrobial molecules of about 1.5 kDa, while BGSP11 produces antimicrobial molecules of 1.5 and 6 kDa active against S. aureus ATCC25923. Amino acid analysis of two antimicrobial molecules (1583.73 Da; from BGSP7 and 1556.31 Da; from BGSP11) revealed that they have a similar composition and differ only by virtue of the presence of a methionine which is present only in BGSP11 molecule. Genome sequencing of the three isolates revealed the presence of gene clusters associated with the production of non-ribosomally synthesized peptides (brevibacillin, bogorol, gramicidin S, plipastatin and tyrocin) and bacteriocins (laterosporulin, a lactococcin 972-like bacteriocin, as well as putative linocin M18, sactipeptide, UviB and lantipeptide-like molecules). Ultimately, the purification of a number of antimicrobial molecules from each isolate suggests that they can be considered as potent biocontrol strains that produce an arsenal of antimicrobial molecules active against Gram-positive and Gram-negative multi-resistant pathogens, fungi and insects.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials",
volume = "14",
number = "5",
doi = "10.1371/journal.pone.0216773"
}

Miljković, M., Jovanović, S., O'Connor, P. M., Mirković, N., Jovčić, B., Filipić, B., Dinić, M., Studholme, D. J., Fira, Đ., Cotter, P. D.,& Kojić, M.. (2019). Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials. in PLoS One
Public Library Science, San Francisco., 14(5).
https://doi.org/10.1371/journal.pone.0216773

Miljković M, Jovanović S, O'Connor PM, Mirković N, Jovčić B, Filipić B, Dinić M, Studholme DJ, Fira Đ, Cotter PD, Kojić M. Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials. in PLoS One. 2019;14(5).
doi:10.1371/journal.pone.0216773 .

Miljković, Marija, Jovanović, Sofija, O'Connor, Paula M., Mirković, Nemanja, Jovčić, Branko, Filipić, Brankica, Dinić, Miroslav, Studholme, David John, Fira, Đorđe, Cotter, Paul D., Kojić, Milan, "Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials" in PLoS One, 14, no. 5 (2019),
https://doi.org/10.1371/journal.pone.0216773 . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Pecikoza, Uroš
AU  - Đokić, Jelena
AU  - Stepanović-Petrović, Radica
AU  - Milenković, Marina
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Lukić, Jovanka
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3152
AB  - The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1 beta and iNOS mRNAs in rat's paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1 beta, TNF-alpha and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Pharmacology
T1  - Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats
VL  - 9
DO  - 10.3389/fphar.2018.00001
ER  - 

@article{
author = "Dinić, Miroslav and Pecikoza, Uroš and Đokić, Jelena and Stepanović-Petrović, Radica and Milenković, Marina and Stevanović, Magdalena and Filipović, Nenad and Begović, Jelena and Golić, Nataša and Lukić, Jovanka",
year = "2018",
abstract = "The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1 beta and iNOS mRNAs in rat's paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1 beta, TNF-alpha and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Pharmacology",
title = "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats",
volume = "9",
doi = "10.3389/fphar.2018.00001"
}

Dinić, M., Pecikoza, U., Đokić, J., Stepanović-Petrović, R., Milenković, M., Stevanović, M., Filipović, N., Begović, J., Golić, N.,& Lukić, J.. (2018). Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats. in Frontiers in Pharmacology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fphar.2018.00001

Dinić M, Pecikoza U, Đokić J, Stepanović-Petrović R, Milenković M, Stevanović M, Filipović N, Begović J, Golić N, Lukić J. Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats. in Frontiers in Pharmacology. 2018;9.
doi:10.3389/fphar.2018.00001 .

Dinić, Miroslav, Pecikoza, Uroš, Đokić, Jelena, Stepanović-Petrović, Radica, Milenković, Marina, Stevanović, Magdalena, Filipović, Nenad, Begović, Jelena, Golić, Nataša, Lukić, Jovanka, "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats" in Frontiers in Pharmacology, 9 (2018),
https://doi.org/10.3389/fphar.2018.00001 . .

TY  - THES
AU  - Dinić, Miroslav
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6030
UR  - http://nardus.mpn.gov.rs/handle/123456789/9986
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:18343/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=2048264546
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3680
AB  - Izolacija i karakterizacija bakterijskih biomolekula koji interaguju sa receptorimaćelija domaćina predstavlja kljuĉ za razumevanje mehanizama probiotiĉkog dejstvalaktobacila. Savremena istraţivanja probiotiĉkih bakterija usmerena su ka identifikacijibiomolekula koji mogu modulisati razliĉite signalne puteve u humanim ćelijama. Svimolekuli koji su poreklom iz probiotika i odgovorni su za njihov pozitivan efekat nazdravlje domaćina nazvani su postbiotici. Zbog slabijeg imunostimulišućeg potencijala,njihova primena predstavlja bezbednu alternativu primeni ţivih bakterija.Ovo istraţivanje je imalo za cilj da testira mogućnost primene postbiotika uublaţavanju simptoma bola i neţeljenih efekata koji nastaju kao posledica primeneanalgetika. Taĉnije, definisana su dva specifiĉna cilja istraţivanja: (i) ispitati uticajbioaktivnih molekula odabranih sojeva laktobacila na proces autofagije u hepatocitima invitro, kao i protektivan efekat ovih molekula kod toksiĉnosti izazvane paracetamolom i (ii)ispitati potencijalni imunomodulatorni efekat egzopolisaharida (EPS-CG11) izolovanog izsoja Lactobacillus paraplantarum BGCG11, u in vivo eksperimentalnim modelimainflamacije. Eksperimenti u kulturi hepatocita ukljuĉili su praćenje stepena oštećenjaHepG2 ćelija izloţenih toksiĉnoj koncentraciji paracetamola i procesa autofagije, sa ciljemidentifikacije potencijalnog mehanizma delovanja postbiotika. Metodološki, vijabilnostHepG2 ćelija analizirana je MTT i LDH esejima. Autofagija je praćena Western blotanalizom odreĊivanjem ekspresije p62/SQSTM1 proteina i akumulacijom liposolubilneforme LC3 proteina. Dodatno, autofagija je analizirana praćenjem ekspresije BECN1, Atg5,p62/SQSTM1 i PINK1 gena i autofagnog fluksa. Za analizu imunomodulatornog efektaEPS-CG11 korišćena su dva in vivo modela inflamacije izazvane karageninom: modelinflamatornog bola i model peritonitisa kod pacova Wistar soja. U eksperimentuinflamatornog bola praćeni su: vremenski tok razvoja hiperalgezije i edema šapica nakonprofilaktiĉke sistemske primene EPS-CG11, ekspresija medijatora inflamacije (IL-1β,TNF-α, IL-6 i iNOS), infiltracija neutrofila (ekspresija MPO enzima) i aktivacija/infiltracijamonocita (ekspresija CD14 markera)...
AB  - Isolation and characterization of bacterial biomolecules involved in the interactionwith the receptors of the host cells represent the key factor for understanding themechanisms of probiotic action of lactobacilli. Novel studies regarding probiotic bacteriahave been focused on the identification of biomolecules which can modulate differentsignaling pathways in human cells. All molecules that originate from probiotics which areresponsible for its positive effects on the host’s health are called postbiotics. Theirapplication represents the safe alternative to the use of live bacteria and itsimmunostimulating potential.This research aimed to test the possibility of using postbiotics in alleviation of painsymptoms and analgesics side effects. More precisely, two main objectives of this researchwere: (i) to examine the influence of bioactive molecules of selected strains of lactobacillion the autophagy process in the hepatocytes, in vitro, as well as protective effect of thesemolecules in paracetamol-induced toxicity and (ii) to examine the potentialimmunomodulatory effect of exopolysaccharide (EPS-CG11) isolated from Lactobacillusparaplantarum BGCG11 strain, in in vivo experimental models of inflammation.Experiments in the hepatocytes culture included monitoring the degree of damage ofHepG2 cells exposed to the toxic paracetamol concentration and the autophagy process,with the aim of identification of potential mechanism of postbiotic action.Methodologically, the cell viability was monitored by MTT and LDH assays. Autophagywas monitored by Western blot analysis, in order to determine the expression of thep62/SQSTM1 protein and the accumulation of the liposoluble form of the LC3 protein.Further, the autophagy was analyzed by monitoring the expression of BECN1, Atg5,p62/SQSTM1 and PINK1 genes and the autophagy flux. For the analysis of theimmunomodulatory effect of EPS-CG11, two in vivo models of carrageenan-inducedinflammation were used: an inflammatory pain model and a peritonitis model in the Wistarrats...
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Uticaj bioaktivnih molekula laktobacila na procese autofagije i inflamacije u in vitro i in vivo sistemima
UR  - https://hdl.handle.net/21.15107/rcub_nardus_9986
ER  - 

@phdthesis{
author = "Dinić, Miroslav",
year = "2018",
abstract = "Izolacija i karakterizacija bakterijskih biomolekula koji interaguju sa receptorimaćelija domaćina predstavlja kljuĉ za razumevanje mehanizama probiotiĉkog dejstvalaktobacila. Savremena istraţivanja probiotiĉkih bakterija usmerena su ka identifikacijibiomolekula koji mogu modulisati razliĉite signalne puteve u humanim ćelijama. Svimolekuli koji su poreklom iz probiotika i odgovorni su za njihov pozitivan efekat nazdravlje domaćina nazvani su postbiotici. Zbog slabijeg imunostimulišućeg potencijala,njihova primena predstavlja bezbednu alternativu primeni ţivih bakterija.Ovo istraţivanje je imalo za cilj da testira mogućnost primene postbiotika uublaţavanju simptoma bola i neţeljenih efekata koji nastaju kao posledica primeneanalgetika. Taĉnije, definisana su dva specifiĉna cilja istraţivanja: (i) ispitati uticajbioaktivnih molekula odabranih sojeva laktobacila na proces autofagije u hepatocitima invitro, kao i protektivan efekat ovih molekula kod toksiĉnosti izazvane paracetamolom i (ii)ispitati potencijalni imunomodulatorni efekat egzopolisaharida (EPS-CG11) izolovanog izsoja Lactobacillus paraplantarum BGCG11, u in vivo eksperimentalnim modelimainflamacije. Eksperimenti u kulturi hepatocita ukljuĉili su praćenje stepena oštećenjaHepG2 ćelija izloţenih toksiĉnoj koncentraciji paracetamola i procesa autofagije, sa ciljemidentifikacije potencijalnog mehanizma delovanja postbiotika. Metodološki, vijabilnostHepG2 ćelija analizirana je MTT i LDH esejima. Autofagija je praćena Western blotanalizom odreĊivanjem ekspresije p62/SQSTM1 proteina i akumulacijom liposolubilneforme LC3 proteina. Dodatno, autofagija je analizirana praćenjem ekspresije BECN1, Atg5,p62/SQSTM1 i PINK1 gena i autofagnog fluksa. Za analizu imunomodulatornog efektaEPS-CG11 korišćena su dva in vivo modela inflamacije izazvane karageninom: modelinflamatornog bola i model peritonitisa kod pacova Wistar soja. U eksperimentuinflamatornog bola praćeni su: vremenski tok razvoja hiperalgezije i edema šapica nakonprofilaktiĉke sistemske primene EPS-CG11, ekspresija medijatora inflamacije (IL-1β,TNF-α, IL-6 i iNOS), infiltracija neutrofila (ekspresija MPO enzima) i aktivacija/infiltracijamonocita (ekspresija CD14 markera)..., Isolation and characterization of bacterial biomolecules involved in the interactionwith the receptors of the host cells represent the key factor for understanding themechanisms of probiotic action of lactobacilli. Novel studies regarding probiotic bacteriahave been focused on the identification of biomolecules which can modulate differentsignaling pathways in human cells. All molecules that originate from probiotics which areresponsible for its positive effects on the host’s health are called postbiotics. Theirapplication represents the safe alternative to the use of live bacteria and itsimmunostimulating potential.This research aimed to test the possibility of using postbiotics in alleviation of painsymptoms and analgesics side effects. More precisely, two main objectives of this researchwere: (i) to examine the influence of bioactive molecules of selected strains of lactobacillion the autophagy process in the hepatocytes, in vitro, as well as protective effect of thesemolecules in paracetamol-induced toxicity and (ii) to examine the potentialimmunomodulatory effect of exopolysaccharide (EPS-CG11) isolated from Lactobacillusparaplantarum BGCG11 strain, in in vivo experimental models of inflammation.Experiments in the hepatocytes culture included monitoring the degree of damage ofHepG2 cells exposed to the toxic paracetamol concentration and the autophagy process,with the aim of identification of potential mechanism of postbiotic action.Methodologically, the cell viability was monitored by MTT and LDH assays. Autophagywas monitored by Western blot analysis, in order to determine the expression of thep62/SQSTM1 protein and the accumulation of the liposoluble form of the LC3 protein.Further, the autophagy was analyzed by monitoring the expression of BECN1, Atg5,p62/SQSTM1 and PINK1 genes and the autophagy flux. For the analysis of theimmunomodulatory effect of EPS-CG11, two in vivo models of carrageenan-inducedinflammation were used: an inflammatory pain model and a peritonitis model in the Wistarrats...",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Uticaj bioaktivnih molekula laktobacila na procese autofagije i inflamacije u in vitro i in vivo sistemima",
url = "https://hdl.handle.net/21.15107/rcub_nardus_9986"
}

Dinić, M.. (2018). Uticaj bioaktivnih molekula laktobacila na procese autofagije i inflamacije u in vitro i in vivo sistemima. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_9986

Dinić M. Uticaj bioaktivnih molekula laktobacila na procese autofagije i inflamacije u in vitro i in vivo sistemima. in Универзитет у Београду. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_9986 .

Dinić, Miroslav, "Uticaj bioaktivnih molekula laktobacila na procese autofagije i inflamacije u in vitro i in vivo sistemima" in Универзитет у Београду (2018),
https://hdl.handle.net/21.15107/rcub_nardus_9986 .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Lukić, Jovanka
AU  - Đokić, Jelena
AU  - Milenković, Marina
AU  - Strahinić, Ivana
AU  - Golić, Nataša
AU  - Begović, Jelena
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2917
AB  - The aim of this study was to investigate the potential of postbiotics originated from Lactobacillus fermentum BGHV110 strain (HV110) to counteract acetaminophen (APAP)-induced hepatotoxicity in HepG2 cells. This strain was selected according to its autophagy inducing potential, based on previous studies reporting protective role of autophagy in APAP caused cellular damage. Cell viability was assessed using MTT and LDH assays, while autophagy was monitored by qPCR analysis of BECN1, Atg5, p62/SQSTM1, and PINK1 mRNA expression and by Western blot analysis of p62/SQSTM1 and lipidated LC3 accumulation. Our results showed that detrimental effect of APAP on cell viability was suppressed in the presence of HV110 which was linked with increased conversion of LC3 protein and p62/SQSTM1 protein degradation. Additionally, higher p62/SQSTM1 and PINK1 mRNA transcription were noticed in cells co-treated with APAP/HV110, simultaneously. In conclusion, this study suggests that HV110 enhances activation of PINK1-dependent autophagy in HepG2 cells and its eventual co-supplementation with APAP could be potentially used for alleviation of hepatotoxic side effects caused by APAP overdose.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity
VL  - 8
DO  - 10.3389/fmicb.2017.00594
ER  - 

@article{
author = "Dinić, Miroslav and Lukić, Jovanka and Đokić, Jelena and Milenković, Marina and Strahinić, Ivana and Golić, Nataša and Begović, Jelena",
year = "2017",
abstract = "The aim of this study was to investigate the potential of postbiotics originated from Lactobacillus fermentum BGHV110 strain (HV110) to counteract acetaminophen (APAP)-induced hepatotoxicity in HepG2 cells. This strain was selected according to its autophagy inducing potential, based on previous studies reporting protective role of autophagy in APAP caused cellular damage. Cell viability was assessed using MTT and LDH assays, while autophagy was monitored by qPCR analysis of BECN1, Atg5, p62/SQSTM1, and PINK1 mRNA expression and by Western blot analysis of p62/SQSTM1 and lipidated LC3 accumulation. Our results showed that detrimental effect of APAP on cell viability was suppressed in the presence of HV110 which was linked with increased conversion of LC3 protein and p62/SQSTM1 protein degradation. Additionally, higher p62/SQSTM1 and PINK1 mRNA transcription were noticed in cells co-treated with APAP/HV110, simultaneously. In conclusion, this study suggests that HV110 enhances activation of PINK1-dependent autophagy in HepG2 cells and its eventual co-supplementation with APAP could be potentially used for alleviation of hepatotoxic side effects caused by APAP overdose.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity",
volume = "8",
doi = "10.3389/fmicb.2017.00594"
}

Dinić, M., Lukić, J., Đokić, J., Milenković, M., Strahinić, I., Golić, N.,& Begović, J.. (2017). Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fmicb.2017.00594

Dinić M, Lukić J, Đokić J, Milenković M, Strahinić I, Golić N, Begović J. Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.00594 .

Dinić, Miroslav, Lukić, Jovanka, Đokić, Jelena, Milenković, Marina, Strahinić, Ivana, Golić, Nataša, Begović, Jelena, "Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.00594 . .

TY  - JOUR
AU  - Kolundžić, Marina
AU  - Groznanić, Nađa
AU  - Stanojković, Tatjana
AU  - Milenković, Marina
AU  - Dinić, Miroslav
AU  - Golić, Nataša
AU  - Kojić, Milan
AU  - Kundaković, Tatjana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2755
AB  - Cyclohexane, dichloromethane, and methanol extracts of the medicinal mushroom Laetiporus sulphureus were tested for their antimicrobial, cytotoxic, and radical scavenging activities. Total polyphenol content was also determined. Regarding the amount of the phenolic compounds in the methanol extract, there were 14.1 +/- 0.6 mg gallic acid/g dry extract and a concentration of 0.66 +/- 0.15 mg/mL resulted in the removal of 50% of the DPPH radicals (half-maximal inhibitory concentration [IC50]) . The average minimum inhibitory concentrations (MICs) against a select panel of microorganisms were between 125 and 250 mu g/mL, and the methanolic extract significantly inhibited the growth of Staphylococcus aureus (MIC, 31.25 mu g/mL). Also, cyclohexane and dichloromethane extracts inhibited the growth of Helicobacter pylori (MIC, 62.5 mu g/mL). Cyclohexane and dichloromethane extracts showed higher antiproliferative action compared with the methanol extract. The lowest IC50 was induced on HeLa cells by dichloromethane extract (IC50,67.20 +/- 5.49 mu g/mL), followed by cyclohexane extract (IC50 75.91 +/- 1.57 mu g/mL). The tested cyclohexane and dichloromethane extracts possessed moderate antibacterial and significant cytotoxic activity against both malignant (HeLa and N87) and healthy (MRC5) cell lines. Methanol extracts exhibited promising anti-Helicobacter activity without cytotoxicity against N87 and MRC5 cell lines.
PB  - Begell House Inc, Danbury
T2  - International Journal of Medicinal Mushrooms
T1  - Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia
VL  - 18
IS  - 6
SP  - 469
EP  - 476
DO  - 10.1615/IntJMedMushrooms.v18.i6.10
ER  - 

@article{
author = "Kolundžić, Marina and Groznanić, Nađa and Stanojković, Tatjana and Milenković, Marina and Dinić, Miroslav and Golić, Nataša and Kojić, Milan and Kundaković, Tatjana",
year = "2016",
abstract = "Cyclohexane, dichloromethane, and methanol extracts of the medicinal mushroom Laetiporus sulphureus were tested for their antimicrobial, cytotoxic, and radical scavenging activities. Total polyphenol content was also determined. Regarding the amount of the phenolic compounds in the methanol extract, there were 14.1 +/- 0.6 mg gallic acid/g dry extract and a concentration of 0.66 +/- 0.15 mg/mL resulted in the removal of 50% of the DPPH radicals (half-maximal inhibitory concentration [IC50]) . The average minimum inhibitory concentrations (MICs) against a select panel of microorganisms were between 125 and 250 mu g/mL, and the methanolic extract significantly inhibited the growth of Staphylococcus aureus (MIC, 31.25 mu g/mL). Also, cyclohexane and dichloromethane extracts inhibited the growth of Helicobacter pylori (MIC, 62.5 mu g/mL). Cyclohexane and dichloromethane extracts showed higher antiproliferative action compared with the methanol extract. The lowest IC50 was induced on HeLa cells by dichloromethane extract (IC50,67.20 +/- 5.49 mu g/mL), followed by cyclohexane extract (IC50 75.91 +/- 1.57 mu g/mL). The tested cyclohexane and dichloromethane extracts possessed moderate antibacterial and significant cytotoxic activity against both malignant (HeLa and N87) and healthy (MRC5) cell lines. Methanol extracts exhibited promising anti-Helicobacter activity without cytotoxicity against N87 and MRC5 cell lines.",
publisher = "Begell House Inc, Danbury",
journal = "International Journal of Medicinal Mushrooms",
title = "Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia",
volume = "18",
number = "6",
pages = "469-476",
doi = "10.1615/IntJMedMushrooms.v18.i6.10"
}

Kolundžić, M., Groznanić, N., Stanojković, T., Milenković, M., Dinić, M., Golić, N., Kojić, M.,& Kundaković, T.. (2016). Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia. in International Journal of Medicinal Mushrooms
Begell House Inc, Danbury., 18(6), 469-476.
https://doi.org/10.1615/IntJMedMushrooms.v18.i6.10

Kolundžić M, Groznanić N, Stanojković T, Milenković M, Dinić M, Golić N, Kojić M, Kundaković T. Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia. in International Journal of Medicinal Mushrooms. 2016;18(6):469-476.
doi:10.1615/IntJMedMushrooms.v18.i6.10 .

Kolundžić, Marina, Groznanić, Nađa, Stanojković, Tatjana, Milenković, Marina, Dinić, Miroslav, Golić, Nataša, Kojić, Milan, Kundaković, Tatjana, "Antimicrobial and Cytotoxic Activities of the Sulphur Shelf Medicinal Mushroom, Laetiporus sulphureus (Agaricomycetes), from Serbia" in International Journal of Medicinal Mushrooms, 18, no. 6 (2016):469-476,
https://doi.org/10.1615/IntJMedMushrooms.v18.i6.10 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Dinić, Miroslav
AU  - Marčetić, Mirjana
AU  - Milenković, Marina
AU  - Begović, Jelena
AU  - Strahinić, Ivana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2645
AB  - Aim of this work was to assess the metabolic and physiological changes that occurred in the hind gut of rats after feeding with soybean carbohydrates alone and in combination with Lactobacillus helveticus BGRA43. Wistar rats were gavaged with soybean flour for 28 days. The parameters assessed included fecal volatile organic compounds, and L-lactate, reducing sugars, proteins, ammonia and water levels in the colonic lumen. The presence of lactic acid (LAB), sulfate reducing (SRB) and methanogenic bacteria was assessed by semi-quantitative PCR. Malondialdehyde levels as well as lymphoid tissue size in ileal and colonic mucosa were also evaluated. On the basics of the results obtained, correlation network was created, setting the parameters tested in research in two metabolic groups: saccharolytic and proteolytic fermentation group. The principal finding of the study is a negative correlation between oral administration of BGRA43 and increase of parameters related to carbohydrate fermentation in the gut, and a positive correlation to factors related to proteolytic fermentation. On the contrary, soybean carbohydrates were correlated with increased values of factors related to carbohydrate catabolism. Different effects of BGRA43 and soybean carbohydrates on metabolic processes in colonic lumen indicate the possibility of applying the BGRA43 in alleviating the gastrointestinal symptoms occurring after consuming hardly digestible carbohydrates.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika, Belgrade
T1  - Effects of soybean carbohydrates and Lactobacillus helveticus BGRA43 on metabolic processes in rat colon
VL  - 48
IS  - 3
SP  - 903
EP  - 921
DO  - 10.2298/GENSR1603903L
ER  - 

@article{
author = "Lukić, Jovanka and Dinić, Miroslav and Marčetić, Mirjana and Milenković, Marina and Begović, Jelena and Strahinić, Ivana",
year = "2016",
abstract = "Aim of this work was to assess the metabolic and physiological changes that occurred in the hind gut of rats after feeding with soybean carbohydrates alone and in combination with Lactobacillus helveticus BGRA43. Wistar rats were gavaged with soybean flour for 28 days. The parameters assessed included fecal volatile organic compounds, and L-lactate, reducing sugars, proteins, ammonia and water levels in the colonic lumen. The presence of lactic acid (LAB), sulfate reducing (SRB) and methanogenic bacteria was assessed by semi-quantitative PCR. Malondialdehyde levels as well as lymphoid tissue size in ileal and colonic mucosa were also evaluated. On the basics of the results obtained, correlation network was created, setting the parameters tested in research in two metabolic groups: saccharolytic and proteolytic fermentation group. The principal finding of the study is a negative correlation between oral administration of BGRA43 and increase of parameters related to carbohydrate fermentation in the gut, and a positive correlation to factors related to proteolytic fermentation. On the contrary, soybean carbohydrates were correlated with increased values of factors related to carbohydrate catabolism. Different effects of BGRA43 and soybean carbohydrates on metabolic processes in colonic lumen indicate the possibility of applying the BGRA43 in alleviating the gastrointestinal symptoms occurring after consuming hardly digestible carbohydrates.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika, Belgrade",
title = "Effects of soybean carbohydrates and Lactobacillus helveticus BGRA43 on metabolic processes in rat colon",
volume = "48",
number = "3",
pages = "903-921",
doi = "10.2298/GENSR1603903L"
}

Lukić, J., Dinić, M., Marčetić, M., Milenković, M., Begović, J.,& Strahinić, I.. (2016). Effects of soybean carbohydrates and Lactobacillus helveticus BGRA43 on metabolic processes in rat colon. in Genetika, Belgrade
Društvo genetičara Srbije, Beograd., 48(3), 903-921.
https://doi.org/10.2298/GENSR1603903L

Lukić J, Dinić M, Marčetić M, Milenković M, Begović J, Strahinić I. Effects of soybean carbohydrates and Lactobacillus helveticus BGRA43 on metabolic processes in rat colon. in Genetika, Belgrade. 2016;48(3):903-921.
doi:10.2298/GENSR1603903L .

Lukić, Jovanka, Dinić, Miroslav, Marčetić, Mirjana, Milenković, Marina, Begović, Jelena, Strahinić, Ivana, "Effects of soybean carbohydrates and Lactobacillus helveticus BGRA43 on metabolic processes in rat colon" in Genetika, Belgrade, 48, no. 3 (2016):903-921,
https://doi.org/10.2298/GENSR1603903L . .