TY  - JOUR
AU  - Antunović, Marko
AU  - Vučinić, Slavica
AU  - Kotur-Stevuljević, Jelena
AU  - Krstić, Kristijan
AU  - Jović-Stošić, Jasmina
AU  - Kilibarda, Vesna
AU  - Perković-Vukčević, Nataša
AU  - Đorđević, Snežana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5415
AB  - Purpose: The aim of this study was to analyze data on gabapentinoid-related attendances to the National Poison Control Center of Serbia (NPCC), particularly abuse cases; to estimate its changes and to compare it with trends in national consumption rates of these drugs. We also aimed to analyze the main characteristics of the study population and to investigate the major clinical effects in poisoned patients. Patients and Methods: This is a retrospective study of patients admitted to the NPCC for acute poisoning involving gabapentinoids from 1 May 2012 to 1 October 2022. Results: There were 357 (95.5%) pregabalin-related and 17 (4.5%) gabapentin-related poisoning cases in 302 patients. Abuse of pregabalin was detected in 27.8% (84/302), while gabapentin abuse occurred in 0.7% (2/302) of all patients. A steady increase in rates of pregabalin poisoning and abuse cases strongly correlated with the increase in overall consumption of this drug, while there were no significant changes in rates of gabapentin consumption, poisoning and abuse rate during the study period. Most patients who abused pregabalin pregabalin were males (84.5%) and the median age was 26 years (range: 15–45 years). Almost 60% of patients who abused pregabalin (48/84) belonged to the migrant population. Co-ingestions occurred in 89.4% of pregabalin-related cases (319/357), resulting in more severe poisoning. The most often co-ingested drugs were benzodiazepines and among them clonazepam was detected in the largest number of cases. Conclusion: The poisoning and abuse cases involving pregabalin are on the rise in Serbia, which coincided with an increase in its overall consumption during the study period. Isolated pregabalin ingestions resulted in mild poisoning, although severe symptoms such as coma and bradycardia were recorded. When prescribing pregabalin to patients at risk of abuse caution is needed. Strengthening the measures for dispensing of pregabalin may reduce the risks associated with its abuse.
PB  - Dove Medical Press Ltd
T2  - International Journal of General Medicine
T1  - Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study
VL  - 16
SP  - 1239
EP  - 1250
DO  - 10.2147/IJGM.S405616
ER  - 

@article{
author = "Antunović, Marko and Vučinić, Slavica and Kotur-Stevuljević, Jelena and Krstić, Kristijan and Jović-Stošić, Jasmina and Kilibarda, Vesna and Perković-Vukčević, Nataša and Đorđević, Snežana",
year = "2023",
abstract = "Purpose: The aim of this study was to analyze data on gabapentinoid-related attendances to the National Poison Control Center of Serbia (NPCC), particularly abuse cases; to estimate its changes and to compare it with trends in national consumption rates of these drugs. We also aimed to analyze the main characteristics of the study population and to investigate the major clinical effects in poisoned patients. Patients and Methods: This is a retrospective study of patients admitted to the NPCC for acute poisoning involving gabapentinoids from 1 May 2012 to 1 October 2022. Results: There were 357 (95.5%) pregabalin-related and 17 (4.5%) gabapentin-related poisoning cases in 302 patients. Abuse of pregabalin was detected in 27.8% (84/302), while gabapentin abuse occurred in 0.7% (2/302) of all patients. A steady increase in rates of pregabalin poisoning and abuse cases strongly correlated with the increase in overall consumption of this drug, while there were no significant changes in rates of gabapentin consumption, poisoning and abuse rate during the study period. Most patients who abused pregabalin pregabalin were males (84.5%) and the median age was 26 years (range: 15–45 years). Almost 60% of patients who abused pregabalin (48/84) belonged to the migrant population. Co-ingestions occurred in 89.4% of pregabalin-related cases (319/357), resulting in more severe poisoning. The most often co-ingested drugs were benzodiazepines and among them clonazepam was detected in the largest number of cases. Conclusion: The poisoning and abuse cases involving pregabalin are on the rise in Serbia, which coincided with an increase in its overall consumption during the study period. Isolated pregabalin ingestions resulted in mild poisoning, although severe symptoms such as coma and bradycardia were recorded. When prescribing pregabalin to patients at risk of abuse caution is needed. Strengthening the measures for dispensing of pregabalin may reduce the risks associated with its abuse.",
publisher = "Dove Medical Press Ltd",
journal = "International Journal of General Medicine",
title = "Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study",
volume = "16",
pages = "1239-1250",
doi = "10.2147/IJGM.S405616"
}

Antunović, M., Vučinić, S., Kotur-Stevuljević, J., Krstić, K., Jović-Stošić, J., Kilibarda, V., Perković-Vukčević, N.,& Đorđević, S.. (2023). Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study. in International Journal of General Medicine
Dove Medical Press Ltd., 16, 1239-1250.
https://doi.org/10.2147/IJGM.S405616

Antunović M, Vučinić S, Kotur-Stevuljević J, Krstić K, Jović-Stošić J, Kilibarda V, Perković-Vukčević N, Đorđević S. Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study. in International Journal of General Medicine. 2023;16:1239-1250.
doi:10.2147/IJGM.S405616 .

Antunović, Marko, Vučinić, Slavica, Kotur-Stevuljević, Jelena, Krstić, Kristijan, Jović-Stošić, Jasmina, Kilibarda, Vesna, Perković-Vukčević, Nataša, Đorđević, Snežana, "Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study" in International Journal of General Medicine, 16 (2023):1239-1250,
https://doi.org/10.2147/IJGM.S405616 . .

TY  - CONF
AU  - Ćurčić, Marijana
AU  - Blagojević, Zorica
AU  - Milenković, Milan
AU  - Baralić, Katarina
AU  - Vukelić, Dragana
AU  - Antonijević-Miljaković, Evica
AU  - Đukić-Ćosić, Danijela
AU  - Đorđević, Snežana
AU  - Antonijević, Biljana
AU  - Bulat, Zorica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5438
AB  - Materijali i predmeti u kontaktu sa hranom mogu da sadrže bisfenol A koji se dodaje kao plastifikator
ili antioksidans pri izradi plastike ali je i sastavni deo epoksi smola koje se koriste kao unutrašnji
premazi za konzerve u kojima se čuvaju namirnice. ...
AB  - Food contact materials can contain bisphenol A added as a plasticizer or antioxidant as well as a component of epoxy resins that are a coating material for food cans. ...
PB  - Udruženje toksikologa Srbije
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book
T1  - Validacija metode tečne hromatografije sa fluorescentim detektorom (HPLC-FLD) za određivanja bisfenola a u materijalima i predmetima koji dolaze u kontakt sa hranom
T1  - Validation of the high-performance liquid chromatography with fluorescence detection method (HPLC-FLD) for the determination of bisphenol a in food contact material
SP  - 13
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5438
ER  - 

@conference{
author = "Ćurčić, Marijana and Blagojević, Zorica and Milenković, Milan and Baralić, Katarina and Vukelić, Dragana and Antonijević-Miljaković, Evica and Đukić-Ćosić, Danijela and Đorđević, Snežana and Antonijević, Biljana and Bulat, Zorica",
year = "2023",
abstract = "Materijali i predmeti u kontaktu sa hranom mogu da sadrže bisfenol A koji se dodaje kao plastifikator
ili antioksidans pri izradi plastike ali je i sastavni deo epoksi smola koje se koriste kao unutrašnji
premazi za konzerve u kojima se čuvaju namirnice. ..., Food contact materials can contain bisphenol A added as a plasticizer or antioxidant as well as a component of epoxy resins that are a coating material for food cans. ...",
publisher = "Udruženje toksikologa Srbije",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book",
title = "Validacija metode tečne hromatografije sa fluorescentim detektorom (HPLC-FLD) za određivanja bisfenola a u materijalima i predmetima koji dolaze u kontakt sa hranom, Validation of the high-performance liquid chromatography with fluorescence detection method (HPLC-FLD) for the determination of bisphenol a in food contact material",
pages = "13-14",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5438"
}

Ćurčić, M., Blagojević, Z., Milenković, M., Baralić, K., Vukelić, D., Antonijević-Miljaković, E., Đukić-Ćosić, D., Đorđević, S., Antonijević, B.,& Bulat, Z.. (2023). Validacija metode tečne hromatografije sa fluorescentim detektorom (HPLC-FLD) za određivanja bisfenola a u materijalima i predmetima koji dolaze u kontakt sa hranom. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book
Udruženje toksikologa Srbije., 13-14.
https://hdl.handle.net/21.15107/rcub_farfar_5438

Ćurčić M, Blagojević Z, Milenković M, Baralić K, Vukelić D, Antonijević-Miljaković E, Đukić-Ćosić D, Đorđević S, Antonijević B, Bulat Z. Validacija metode tečne hromatografije sa fluorescentim detektorom (HPLC-FLD) za određivanja bisfenola a u materijalima i predmetima koji dolaze u kontakt sa hranom. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book. 2023;:13-14.
https://hdl.handle.net/21.15107/rcub_farfar_5438 .

Ćurčić, Marijana, Blagojević, Zorica, Milenković, Milan, Baralić, Katarina, Vukelić, Dragana, Antonijević-Miljaković, Evica, Đukić-Ćosić, Danijela, Đorđević, Snežana, Antonijević, Biljana, Bulat, Zorica, "Validacija metode tečne hromatografije sa fluorescentim detektorom (HPLC-FLD) za određivanja bisfenola a u materijalima i predmetima koji dolaze u kontakt sa hranom" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book (2023):13-14,
https://hdl.handle.net/21.15107/rcub_farfar_5438 .

TY  - CONF
AU  - Šuić, Ana
AU  - Javorac, Dragana
AU  - Baralić, Katarina
AU  - Marić, Đurđica
AU  - Antonijević-Miljaković, Evica
AU  - Buha-Đorđević, Aleksandra
AU  - Ćurčić, Marijana
AU  - Đorđević, Snežana
AU  - Đukić-Ćosić, Danijela
AU  - Antonijević, Biljana
AU  - Bulat, Zorica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4640
AB  - According to the current regulations in Serbia, the cultivation and sale of hemp-based
products (Cannabis sativa L.) containing up to 0.3% of substances from the group of
tetrahydrocannabinol is allowed. ∆-9-tetrahydrocannabinol (THC) is responsible for the
psychoactive effects and the development of cannabis addiction. A growing number of products
based on parts or the whole cannabis plant are on free sale. Since cannabis is the most
commonly used psychoactive substance, there is a greater need for quick and easy detection
in the body. The Aim of this project was to examine the effect of commercial cannabis-based
products consuption on urine THC detection by using immunochromatographic test strips.
Eight healthy volunteers consumed commercially available hemp-based products (four tea
products, two ground and two whole seeds products). The products were consumed according to
the instructions given by the manufacturer, for three consecutive days, and on the fourth day the
first morning urine was tested for the presence of THC using multicomponent One Step Rapid
Test®-Ameritek® test strips. Urine samples from five subjects gave a negative test result, and three
samples, from subjects who consumed hemp ground seeds and tea in bulk, gave a positive THC
test. Further analysis of positive urine samples by the conformative method (HPLC-PDA) did not
prove the presence of THC. Consumption of certain commercial cannabis-based products can lead
to false-positive results in the detection of THC in urine by immunochromatographic test strips.
Therefore, it is necessary to perform further analysis of urine by conformative methods.
AB  - Prema važećoj regulativi u Srbiji je dozvoljeno gajenje i prodaja proizvoda na bazi
konoplje (Cannabis sativa L.) koji sadrže do 0,3% supstanci iz grupe tetrahidrokanabinola od
kojih se ∆-9-tetrahidrokanabinolu (THC) pripisuju psihoaktivni efekti i razvoj zavisnosti
povezan sa zloupotrebom kanabisa. U slobodnoj prodaji se nalazi sve veći broj proizvoda
baziranih na delovima ili celoj biljci kanabisa. S obzirom na to da je kanabis najčešće
korišćena psihoaktivna supstanca postoji i veća potreba za brzom i lakom detekcijom u
organizmu. Cilj ovog rada je ispitati uticaj konzumiranja komercijalnih proizvoda na bazi
konoplje na dokazivanje THC u urinu imunohromatografskim test trakama (1,2). U
ispitivanje je uključeno osam zdravih dobrovoljaca koji su konzumirali komercijalno
dostupne preparate na bazi konoplje (četiri čaja, dva mlevena i dva cela semena). Preparati
su konzumirani prema uputstvu datom od strane proizvođača, tri uzastopna dana, a četvrtog
dana je vršeno testiranje prvog jutarnjeg urina na prisustvo THC, upotrebom
višekomponentnih One Step Rapid Test®‐Ameritek® test traka. Uzorci pet ispitanika su dali
negativan rezultat testa, a tri uzorka, poreklom od ispitanika koji su konzumirali mleveno
seme konoplje i čaj u rinfuzu, dala su pozitivan test na THC. Daljom analizom urina koji su
dali pozitivan rezultat, konfirmativnom metodom (HPLC-PDA) nije dokazano prisustvo THC.
Konzumacija pojedinih preparata na bazi konoplje, koji se mogu naći u slobodnoj prodaji,
može da dovede do pojave lažno pozitivnih rezultata pri dokazivanju THC u urinu
imunohromatografskim test trakama, zbog čega je neophodno izvršiti dalju analizu urina
konfirmativnim metodama.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Influence of consumption of commercial hemp-based products on the detection of tetrahydrocanabinol in urine by immunochromatographic test strips
T1  - Uticaj konzumiranja komercijalnih proizvoda na bazi konoplje na dokazivanje tetrahidrokanabinola imunohromatografskim test trakama u urinu
VL  - 72
IS  - 4 suplement
SP  - S605
EP  - S606
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4640
ER  - 

@conference{
author = "Šuić, Ana and Javorac, Dragana and Baralić, Katarina and Marić, Đurđica and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Ćurčić, Marijana and Đorđević, Snežana and Đukić-Ćosić, Danijela and Antonijević, Biljana and Bulat, Zorica",
year = "2022",
abstract = "According to the current regulations in Serbia, the cultivation and sale of hemp-based
products (Cannabis sativa L.) containing up to 0.3% of substances from the group of
tetrahydrocannabinol is allowed. ∆-9-tetrahydrocannabinol (THC) is responsible for the
psychoactive effects and the development of cannabis addiction. A growing number of products
based on parts or the whole cannabis plant are on free sale. Since cannabis is the most
commonly used psychoactive substance, there is a greater need for quick and easy detection
in the body. The Aim of this project was to examine the effect of commercial cannabis-based
products consuption on urine THC detection by using immunochromatographic test strips.
Eight healthy volunteers consumed commercially available hemp-based products (four tea
products, two ground and two whole seeds products). The products were consumed according to
the instructions given by the manufacturer, for three consecutive days, and on the fourth day the
first morning urine was tested for the presence of THC using multicomponent One Step Rapid
Test®-Ameritek® test strips. Urine samples from five subjects gave a negative test result, and three
samples, from subjects who consumed hemp ground seeds and tea in bulk, gave a positive THC
test. Further analysis of positive urine samples by the conformative method (HPLC-PDA) did not
prove the presence of THC. Consumption of certain commercial cannabis-based products can lead
to false-positive results in the detection of THC in urine by immunochromatographic test strips.
Therefore, it is necessary to perform further analysis of urine by conformative methods., Prema važećoj regulativi u Srbiji je dozvoljeno gajenje i prodaja proizvoda na bazi
konoplje (Cannabis sativa L.) koji sadrže do 0,3% supstanci iz grupe tetrahidrokanabinola od
kojih se ∆-9-tetrahidrokanabinolu (THC) pripisuju psihoaktivni efekti i razvoj zavisnosti
povezan sa zloupotrebom kanabisa. U slobodnoj prodaji se nalazi sve veći broj proizvoda
baziranih na delovima ili celoj biljci kanabisa. S obzirom na to da je kanabis najčešće
korišćena psihoaktivna supstanca postoji i veća potreba za brzom i lakom detekcijom u
organizmu. Cilj ovog rada je ispitati uticaj konzumiranja komercijalnih proizvoda na bazi
konoplje na dokazivanje THC u urinu imunohromatografskim test trakama (1,2). U
ispitivanje je uključeno osam zdravih dobrovoljaca koji su konzumirali komercijalno
dostupne preparate na bazi konoplje (četiri čaja, dva mlevena i dva cela semena). Preparati
su konzumirani prema uputstvu datom od strane proizvođača, tri uzastopna dana, a četvrtog
dana je vršeno testiranje prvog jutarnjeg urina na prisustvo THC, upotrebom
višekomponentnih One Step Rapid Test®‐Ameritek® test traka. Uzorci pet ispitanika su dali
negativan rezultat testa, a tri uzorka, poreklom od ispitanika koji su konzumirali mleveno
seme konoplje i čaj u rinfuzu, dala su pozitivan test na THC. Daljom analizom urina koji su
dali pozitivan rezultat, konfirmativnom metodom (HPLC-PDA) nije dokazano prisustvo THC.
Konzumacija pojedinih preparata na bazi konoplje, koji se mogu naći u slobodnoj prodaji,
može da dovede do pojave lažno pozitivnih rezultata pri dokazivanju THC u urinu
imunohromatografskim test trakama, zbog čega je neophodno izvršiti dalju analizu urina
konfirmativnim metodama.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Influence of consumption of commercial hemp-based products on the detection of tetrahydrocanabinol in urine by immunochromatographic test strips, Uticaj konzumiranja komercijalnih proizvoda na bazi konoplje na dokazivanje tetrahidrokanabinola imunohromatografskim test trakama u urinu",
volume = "72",
number = "4 suplement",
pages = "S605-S606",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4640"
}

Šuić, A., Javorac, D., Baralić, K., Marić, Đ., Antonijević-Miljaković, E., Buha-Đorđević, A., Ćurčić, M., Đorđević, S., Đukić-Ćosić, D., Antonijević, B.,& Bulat, Z.. (2022). Influence of consumption of commercial hemp-based products on the detection of tetrahydrocanabinol in urine by immunochromatographic test strips. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S605-S606.
https://hdl.handle.net/21.15107/rcub_farfar_4640

Šuić A, Javorac D, Baralić K, Marić Đ, Antonijević-Miljaković E, Buha-Đorđević A, Ćurčić M, Đorđević S, Đukić-Ćosić D, Antonijević B, Bulat Z. Influence of consumption of commercial hemp-based products on the detection of tetrahydrocanabinol in urine by immunochromatographic test strips. in Arhiv za farmaciju. 2022;72(4 suplement):S605-S606.
https://hdl.handle.net/21.15107/rcub_farfar_4640 .

Šuić, Ana, Javorac, Dragana, Baralić, Katarina, Marić, Đurđica, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Ćurčić, Marijana, Đorđević, Snežana, Đukić-Ćosić, Danijela, Antonijević, Biljana, Bulat, Zorica, "Influence of consumption of commercial hemp-based products on the detection of tetrahydrocanabinol in urine by immunochromatographic test strips" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S605-S606,
https://hdl.handle.net/21.15107/rcub_farfar_4640 .

TY  - JOUR
AU  - Džudović, Jelena
AU  - Crevar-Sakač, Milkica
AU  - Antunović, Marko
AU  - Repić, Aleksandra
AU  - Obradović, Slobodan
AU  - Đorđević, Snežana
AU  - Savić, Jelena
AU  - Džudović, Boris
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4332
AB  - Oral anticoagulants are a group of drugs used for the prevention and treatment of venous thrombosis and venous thromboembolism. For the last ten years, direct oral anticoagulants (DOAC) have been available and are equally effective, but significantly safer than vitamin K antagonists. In the case of an overdose, their most important side effect is still bleeding. Due to their widespread use, as well as increased toxicological importance there is a need to develop an analytical method for the determination of DOAC in biological material. The aim of this paper was to establish a method for the quantification of apixaban as one of the representatives of DOAC. The methodology of the study included the measurement of apixaban in the plasma of patients treated in the intensive care unit. Plasma apixaban concentrations were determined by LC-MS/MS technique using carbamazepine as an internal standard. Obtained validation parameters indicate that the introduced method is sensitive, reliable, precise and accurate. Using this method, apixaban can be quickly and easily detected and quantified in plasma in patients who are suspected of overdosing with this drug.
PB  - Akademiai Kiado ZRt.
T2  - Acta Chromatographica
T1  - Development and validation of LC-MS/MS method for determination of plasma apixaban
VL  - 34
IS  - 3
SP  - 332
EP  - 337
DO  - 10.1556/1326.2021.00948
ER  - 

@article{
author = "Džudović, Jelena and Crevar-Sakač, Milkica and Antunović, Marko and Repić, Aleksandra and Obradović, Slobodan and Đorđević, Snežana and Savić, Jelena and Džudović, Boris",
year = "2022",
abstract = "Oral anticoagulants are a group of drugs used for the prevention and treatment of venous thrombosis and venous thromboembolism. For the last ten years, direct oral anticoagulants (DOAC) have been available and are equally effective, but significantly safer than vitamin K antagonists. In the case of an overdose, their most important side effect is still bleeding. Due to their widespread use, as well as increased toxicological importance there is a need to develop an analytical method for the determination of DOAC in biological material. The aim of this paper was to establish a method for the quantification of apixaban as one of the representatives of DOAC. The methodology of the study included the measurement of apixaban in the plasma of patients treated in the intensive care unit. Plasma apixaban concentrations were determined by LC-MS/MS technique using carbamazepine as an internal standard. Obtained validation parameters indicate that the introduced method is sensitive, reliable, precise and accurate. Using this method, apixaban can be quickly and easily detected and quantified in plasma in patients who are suspected of overdosing with this drug.",
publisher = "Akademiai Kiado ZRt.",
journal = "Acta Chromatographica",
title = "Development and validation of LC-MS/MS method for determination of plasma apixaban",
volume = "34",
number = "3",
pages = "332-337",
doi = "10.1556/1326.2021.00948"
}

Džudović, J., Crevar-Sakač, M., Antunović, M., Repić, A., Obradović, S., Đorđević, S., Savić, J.,& Džudović, B.. (2022). Development and validation of LC-MS/MS method for determination of plasma apixaban. in Acta Chromatographica
Akademiai Kiado ZRt.., 34(3), 332-337.
https://doi.org/10.1556/1326.2021.00948

Džudović J, Crevar-Sakač M, Antunović M, Repić A, Obradović S, Đorđević S, Savić J, Džudović B. Development and validation of LC-MS/MS method for determination of plasma apixaban. in Acta Chromatographica. 2022;34(3):332-337.
doi:10.1556/1326.2021.00948 .

Džudović, Jelena, Crevar-Sakač, Milkica, Antunović, Marko, Repić, Aleksandra, Obradović, Slobodan, Đorđević, Snežana, Savić, Jelena, Džudović, Boris, "Development and validation of LC-MS/MS method for determination of plasma apixaban" in Acta Chromatographica, 34, no. 3 (2022):332-337,
https://doi.org/10.1556/1326.2021.00948 . .

TY  - JOUR
AU  - Antunović, Marko
AU  - Đorđević, Snežana
AU  - Kilibarda, Vesna
AU  - Džudović, Jelena
AU  - Repić, Aleksandra
AU  - Bulat, Zorica
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4498
AB  - Valproic acid (VPA) is antiepileptic drug with a long history of clinical use,
increasingly applied in the treatment of various diseases (bipolar disorder, migraine
prophylaxis, etc). Due to significant inter-individual differences VPA concentration is often
measured in the purpose of therapeutic drug monitoring (TDM) (1). Acute poisonings with
this drug represent a significant problem. According to the American Association of Poison
Control Centers, there has been VPA poisoning incidence increase in the last 20 years.
Reports from the National Poison Control Center in Belgrade list VPA as one of the three
most common causes of antiepileptic drugs intoxications (2). This paper aimed to develop a
modern analytical method for VPA quantification in patient plasma. UPLC/MS method with
fast and simple sample preparation has been validated. After protein precipitation, VPA was
extracted from 100 μL plasma using HLB cartridges. Caprylic acid solution was used as
internal standard. Chromatographic separation was achieved on C18 column (1.8 μm, 2.1 X
150 mm) with gradient elution at constant mobile phase flow. Good peak resolution was
achieved (Rt VPA - 4.73 min, Rt ISTD - 4.94 min). Validation was performed according European
Medicines Agency recommendations. Based on statistical analysism it was shown that the
method is precise, accurate, specific, sensitive and linearity is confirmed in a wide range of
concentrations (1-250 mg / L). Advantages of this method are simple preparation procedure
from a small amount of sample, without prior derivatization and short duration of analysis,
which fully satisfies the needs of TDM and urgent toxicological analyses.
AB  - Valproinska kiselina (VK) je lek iz grupe antiepileptika sa dugom istorijom kliničke
upotrebe, koji se, osim u terapiji epilepsije, poslednjih decenija sve više koristi u terapiji
različitih bolesti (bipolarni poremećaj, profilaksa migrene itd). Zbog značajnih
interindividualnih varijacija, koncentracija VK se često određuje u svrhe terapijskog praćenja
leka (TDM – Therapeutic Drug Monitoring) (1). S druge strane, akutna trovanja ovim lekom
predstavljaju značajan problem. Prema podacima Američkih centara za kontrolu trovanja
došlo je do povećanja incidence trovanja VK u poslednjih 20 godina, dok izveštaji
Nacionalnog centra za kontrolu trovanja u Beogradu navode VK kao jedan od tri najčešća
uzročnika trovanja kada su u pitanju intoksikacije antiepilepticima (2). Cilj ovoga rada je
razvoj savremene analitičke metode za određivanje koncentracije VK u plazmi pacijenata.
Validovana je UPLC/MS metoda koja podrazumeva brzu i jednostavnu pripremu uzorka.
Nakon precipitacije proteina, izvršena je ekstrakcija iz 100 μL plazme uz pomoć HLB
kertridža. Kao interni standard korišćen je rastvor kaprilne kiseline. Hromatografsko
razdvajanje je postignuto na C18 koloni (1,8 μm, 2,1 X 150 mm) gradijentnim eluiranjem pri
konstantnom protoku mobilne faze. Postignuta je dobra rezolucija pikova (Rt VK - 4,73 min,
Rt ISTD - 4,94 min). Validacija je izvedena prema preporukama Evropske agencije za lekove, a
na osnovu statističke analize je pokazano da je metoda precizna, tačna, specifična, osetljiva i
potvrđena je linearnost u širokom opsegu koncentracija (1-250 mg/L). Prednosti ove
metode su jednostavan način pripreme iz male količine uzorka, bez prethodne derivatizacije,
i kratko vreme trajanja analize, što u potpunosti zadovoljava potrebe TDM-a i urgentnih
toksikoloških analiza.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
T2  - Arhiv za farmaciju
T1  - Development and validation of a rapid and simple UPLC/MS method for quantification of therapeutic and toxic valproic acid concentrations in patient plasma
T1  - Razvoj i validacija brze i jednostavne UPLC/MS metode za određivanje terapijskih i toksičnih koncentracija valproinske kiseline u plazmi pacijenata
VL  - 72
IS  - 4 suplement
SP  - S215
EP  - S216
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4498
ER  - 

@article{
author = "Antunović, Marko and Đorđević, Snežana and Kilibarda, Vesna and Džudović, Jelena and Repić, Aleksandra and Bulat, Zorica",
year = "2022",
abstract = "Valproic acid (VPA) is antiepileptic drug with a long history of clinical use,
increasingly applied in the treatment of various diseases (bipolar disorder, migraine
prophylaxis, etc). Due to significant inter-individual differences VPA concentration is often
measured in the purpose of therapeutic drug monitoring (TDM) (1). Acute poisonings with
this drug represent a significant problem. According to the American Association of Poison
Control Centers, there has been VPA poisoning incidence increase in the last 20 years.
Reports from the National Poison Control Center in Belgrade list VPA as one of the three
most common causes of antiepileptic drugs intoxications (2). This paper aimed to develop a
modern analytical method for VPA quantification in patient plasma. UPLC/MS method with
fast and simple sample preparation has been validated. After protein precipitation, VPA was
extracted from 100 μL plasma using HLB cartridges. Caprylic acid solution was used as
internal standard. Chromatographic separation was achieved on C18 column (1.8 μm, 2.1 X
150 mm) with gradient elution at constant mobile phase flow. Good peak resolution was
achieved (Rt VPA - 4.73 min, Rt ISTD - 4.94 min). Validation was performed according European
Medicines Agency recommendations. Based on statistical analysism it was shown that the
method is precise, accurate, specific, sensitive and linearity is confirmed in a wide range of
concentrations (1-250 mg / L). Advantages of this method are simple preparation procedure
from a small amount of sample, without prior derivatization and short duration of analysis,
which fully satisfies the needs of TDM and urgent toxicological analyses., Valproinska kiselina (VK) je lek iz grupe antiepileptika sa dugom istorijom kliničke
upotrebe, koji se, osim u terapiji epilepsije, poslednjih decenija sve više koristi u terapiji
različitih bolesti (bipolarni poremećaj, profilaksa migrene itd). Zbog značajnih
interindividualnih varijacija, koncentracija VK se često određuje u svrhe terapijskog praćenja
leka (TDM – Therapeutic Drug Monitoring) (1). S druge strane, akutna trovanja ovim lekom
predstavljaju značajan problem. Prema podacima Američkih centara za kontrolu trovanja
došlo je do povećanja incidence trovanja VK u poslednjih 20 godina, dok izveštaji
Nacionalnog centra za kontrolu trovanja u Beogradu navode VK kao jedan od tri najčešća
uzročnika trovanja kada su u pitanju intoksikacije antiepilepticima (2). Cilj ovoga rada je
razvoj savremene analitičke metode za određivanje koncentracije VK u plazmi pacijenata.
Validovana je UPLC/MS metoda koja podrazumeva brzu i jednostavnu pripremu uzorka.
Nakon precipitacije proteina, izvršena je ekstrakcija iz 100 μL plazme uz pomoć HLB
kertridža. Kao interni standard korišćen je rastvor kaprilne kiseline. Hromatografsko
razdvajanje je postignuto na C18 koloni (1,8 μm, 2,1 X 150 mm) gradijentnim eluiranjem pri
konstantnom protoku mobilne faze. Postignuta je dobra rezolucija pikova (Rt VK - 4,73 min,
Rt ISTD - 4,94 min). Validacija je izvedena prema preporukama Evropske agencije za lekove, a
na osnovu statističke analize je pokazano da je metoda precizna, tačna, specifična, osetljiva i
potvrđena je linearnost u širokom opsegu koncentracija (1-250 mg/L). Prednosti ove
metode su jednostavan način pripreme iz male količine uzorka, bez prethodne derivatizacije,
i kratko vreme trajanja analize, što u potpunosti zadovoljava potrebe TDM-a i urgentnih
toksikoloških analiza.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Development and validation of a rapid and simple UPLC/MS method for quantification of therapeutic and toxic valproic acid concentrations in patient plasma, Razvoj i validacija brze i jednostavne UPLC/MS metode za određivanje terapijskih i toksičnih koncentracija valproinske kiseline u plazmi pacijenata",
volume = "72",
number = "4 suplement",
pages = "S215-S216",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4498"
}

Antunović, M., Đorđević, S., Kilibarda, V., Džudović, J., Repić, A.,& Bulat, Z.. (2022). Development and validation of a rapid and simple UPLC/MS method for quantification of therapeutic and toxic valproic acid concentrations in patient plasma. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S215-S216.
https://hdl.handle.net/21.15107/rcub_farfar_4498

Antunović M, Đorđević S, Kilibarda V, Džudović J, Repić A, Bulat Z. Development and validation of a rapid and simple UPLC/MS method for quantification of therapeutic and toxic valproic acid concentrations in patient plasma. in Arhiv za farmaciju. 2022;72(4 suplement):S215-S216.
https://hdl.handle.net/21.15107/rcub_farfar_4498 .

Antunović, Marko, Đorđević, Snežana, Kilibarda, Vesna, Džudović, Jelena, Repić, Aleksandra, Bulat, Zorica, "Development and validation of a rapid and simple UPLC/MS method for quantification of therapeutic and toxic valproic acid concentrations in patient plasma" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S215-S216,
https://hdl.handle.net/21.15107/rcub_farfar_4498 .

TY  - JOUR
AU  - Vučinić, Slavica
AU  - Kilibarda, Vesna
AU  - Đorđević, Snežana
AU  - Đorđević, Dragana
AU  - Perković-Vukcević, Nataša
AU  - Vuković-Ercegović, Gordana
AU  - Antonijević, Biljana
AU  - Ćurčić, Marijana
AU  - Antonijević, Evica
AU  - Brajković, Gordana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3196
AB  - A rising number of patients are being treated for overdosing with new psychoactive substances (NPS) available at the illegal drug market in Serbia. The aim of this study was to report clinical and analytical experience of the National Poison Control Centre of Serbia (NPCC) with synthetic cannabinoids (SCs) and point to the NPS available at the illegal drug market in our country. From January 2013 to December 2016, 58 patients (aged between 14 and 25) were treated for the effects of synthetic cannabinoids at the NPCC. Tachycardia was established in 53, mydriasis in 31, somnolence, nausea. vomiting, and agitation in 16, dizziness in 10, disorientation in 9, dyspnoea and chest pain in 4, and loss of consciousness, pallor, paraesthesia, muscle twitches, and short-term memory impairment in 2 patients. After receiving symptomatic and supportive treatment in the emergency ward, all patients had fully recovered within 8 h and were discharged shortly afterwards. Another part of the study was focused on the analysis of the products known under their local street names as "Biljni tamjan" (herbal incense), "Beli slez", and "Rainbow Special" and the analysis of urine sampled from the patients with gas chromatography - mass spectrometry, and high performance liquid chromatography. The detected synthetic cannabinoids were AB-PINACA, JWH-018, JWH-122, JWH-210, 5F-AKB48, and MDMB-CHMICA in herbal products and AB-FUBINACA, AB-CHMINACA, and MDMB-CHIvIICA in the urine samples. Our findings have shown the great capacity of NPCC to I) monitor NPS abuse in Serbia, II) reliably detect SCs in illicit products and biological samples, and III) clinically manage the adverse effects in their users. Future commitments of the NPCC will include systematic collection of relevant data on SCs and their adverse effects, detection of changes in purity and composition of the controlled NPS-based products, and raising the public awareness of NPS to improve the effectiveness of the national Early Warning System.
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - Clinical and analytical experience of the National Poison Control Centre with synthetic cannabinoids
VL  - 69
IS  - 2
SP  - 178
EP  - 185
DO  - 10.2478/aiht-2018-69-3096
ER  - 

@article{
author = "Vučinić, Slavica and Kilibarda, Vesna and Đorđević, Snežana and Đorđević, Dragana and Perković-Vukcević, Nataša and Vuković-Ercegović, Gordana and Antonijević, Biljana and Ćurčić, Marijana and Antonijević, Evica and Brajković, Gordana",
year = "2018",
abstract = "A rising number of patients are being treated for overdosing with new psychoactive substances (NPS) available at the illegal drug market in Serbia. The aim of this study was to report clinical and analytical experience of the National Poison Control Centre of Serbia (NPCC) with synthetic cannabinoids (SCs) and point to the NPS available at the illegal drug market in our country. From January 2013 to December 2016, 58 patients (aged between 14 and 25) were treated for the effects of synthetic cannabinoids at the NPCC. Tachycardia was established in 53, mydriasis in 31, somnolence, nausea. vomiting, and agitation in 16, dizziness in 10, disorientation in 9, dyspnoea and chest pain in 4, and loss of consciousness, pallor, paraesthesia, muscle twitches, and short-term memory impairment in 2 patients. After receiving symptomatic and supportive treatment in the emergency ward, all patients had fully recovered within 8 h and were discharged shortly afterwards. Another part of the study was focused on the analysis of the products known under their local street names as "Biljni tamjan" (herbal incense), "Beli slez", and "Rainbow Special" and the analysis of urine sampled from the patients with gas chromatography - mass spectrometry, and high performance liquid chromatography. The detected synthetic cannabinoids were AB-PINACA, JWH-018, JWH-122, JWH-210, 5F-AKB48, and MDMB-CHMICA in herbal products and AB-FUBINACA, AB-CHMINACA, and MDMB-CHIvIICA in the urine samples. Our findings have shown the great capacity of NPCC to I) monitor NPS abuse in Serbia, II) reliably detect SCs in illicit products and biological samples, and III) clinically manage the adverse effects in their users. Future commitments of the NPCC will include systematic collection of relevant data on SCs and their adverse effects, detection of changes in purity and composition of the controlled NPS-based products, and raising the public awareness of NPS to improve the effectiveness of the national Early Warning System.",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "Clinical and analytical experience of the National Poison Control Centre with synthetic cannabinoids",
volume = "69",
number = "2",
pages = "178-185",
doi = "10.2478/aiht-2018-69-3096"
}

Vučinić, S., Kilibarda, V., Đorđević, S., Đorđević, D., Perković-Vukcević, N., Vuković-Ercegović, G., Antonijević, B., Ćurčić, M., Antonijević, E.,& Brajković, G.. (2018). Clinical and analytical experience of the National Poison Control Centre with synthetic cannabinoids. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Inst Medical Research & Occupational Health, Zagreb., 69(2), 178-185.
https://doi.org/10.2478/aiht-2018-69-3096

Vučinić S, Kilibarda V, Đorđević S, Đorđević D, Perković-Vukcević N, Vuković-Ercegović G, Antonijević B, Ćurčić M, Antonijević E, Brajković G. Clinical and analytical experience of the National Poison Control Centre with synthetic cannabinoids. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2018;69(2):178-185.
doi:10.2478/aiht-2018-69-3096 .

Vučinić, Slavica, Kilibarda, Vesna, Đorđević, Snežana, Đorđević, Dragana, Perković-Vukcević, Nataša, Vuković-Ercegović, Gordana, Antonijević, Biljana, Ćurčić, Marijana, Antonijević, Evica, Brajković, Gordana, "Clinical and analytical experience of the National Poison Control Centre with synthetic cannabinoids" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 69, no. 2 (2018):178-185,
https://doi.org/10.2478/aiht-2018-69-3096 . .

TY  - CONF
AU  - Brajković, Gordana
AU  - Jović-Stošić, Jasmina
AU  - Đorđević, Snežana
AU  - Kilibarda, Vesna
AU  - Ćurčić, Marijana
AU  - Radosavljević-Stevanović, Nataša
AU  - Brajković, Zorica
AU  - Vučinić, Slavica
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2890
PB  - Elsevier Ireland Ltd, Clare
C3  - Toxicology Letters
T1  - Determination of JWH-210, JWH-122 and JWH-081 in urine by Liquid Chromatography Mass Spectrometric method
VL  - 280
IS  - Supplement 1
SP  - S292
EP  - S292
DO  - 10.1016/j.toxlet.2017.07.820
ER  - 

@conference{
author = "Brajković, Gordana and Jović-Stošić, Jasmina and Đorđević, Snežana and Kilibarda, Vesna and Ćurčić, Marijana and Radosavljević-Stevanović, Nataša and Brajković, Zorica and Vučinić, Slavica",
year = "2017",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Toxicology Letters",
title = "Determination of JWH-210, JWH-122 and JWH-081 in urine by Liquid Chromatography Mass Spectrometric method",
volume = "280",
number = "Supplement 1",
pages = "S292-S292",
doi = "10.1016/j.toxlet.2017.07.820"
}

Brajković, G., Jović-Stošić, J., Đorđević, S., Kilibarda, V., Ćurčić, M., Radosavljević-Stevanović, N., Brajković, Z.,& Vučinić, S.. (2017). Determination of JWH-210, JWH-122 and JWH-081 in urine by Liquid Chromatography Mass Spectrometric method. in Toxicology Letters
Elsevier Ireland Ltd, Clare., 280(Supplement 1), S292-S292.
https://doi.org/10.1016/j.toxlet.2017.07.820

Brajković G, Jović-Stošić J, Đorđević S, Kilibarda V, Ćurčić M, Radosavljević-Stevanović N, Brajković Z, Vučinić S. Determination of JWH-210, JWH-122 and JWH-081 in urine by Liquid Chromatography Mass Spectrometric method. in Toxicology Letters. 2017;280(Supplement 1):S292-S292.
doi:10.1016/j.toxlet.2017.07.820 .

Brajković, Gordana, Jović-Stošić, Jasmina, Đorđević, Snežana, Kilibarda, Vesna, Ćurčić, Marijana, Radosavljević-Stevanović, Nataša, Brajković, Zorica, Vučinić, Slavica, "Determination of JWH-210, JWH-122 and JWH-081 in urine by Liquid Chromatography Mass Spectrometric method" in Toxicology Letters, 280, no. Supplement 1 (2017):S292-S292,
https://doi.org/10.1016/j.toxlet.2017.07.820 . .

TY  - JOUR
AU  - Baralić, Katarina
AU  - Kostadinović, Milka
AU  - Antunović, Marko
AU  - Đorđević, Snežana
AU  - Bulat, Zorica
AU  - Ćurčić, Marijana
AU  - Đukić-Ćosić, Danijela
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2554
AB  - This study presents the case of opiate analysis in urine of two female participants, aged between 20 and 25 who consumed commonly available bakery products with poppy seeds. One of the participants consumed strudel, the product with larger amount of poppy seeds, while the other consumed braid pastry. Urine samples of both participants (n=8) were collected 5, 12, 24, 30, 36, 48, 60 and 72 h after the consummation. Immunochromatographic screening test (cuttof 300 ng/mL) and LC-MS analysis were preformed on the presence of opiates. The screening test for opiates was positive for a very long period of time, 48 h after the consumption of braid pastry and even 72 h after the consumption of strudel. LC-MS analysis verified the results of screening test and the presence of the main opioide alcaloides, morphine and codeine, was confirmed in all positive samples. Determined morphine level was higher than the level of codeine in all samples. The concentration of morphine (0.25 mg/L) was higher in urine of the participant who consumed strudel, compared to the concentration of morphine (0.06 mg/L) after the consumption of braid pastry. The results of this research show that opiates can be found in urine after the consumption of products which contain poppy seed. Morphine and codeine can be detected even during the same time period as in the samples of users of psychoactive substances (3 days and longer). According to the results, retention time of opiates in urine is longer after the consumption of products with higher amounts of poppy. This finding should be taken into account in routine testing of employees in the workplace, members of the military, students in schools, as well as athletes.
AB  - U ovom radu je prikazan slučaj dve osobe ženskog pola starosti između 20 i 25 godina, koje su konzumirale široko dostupne pekarske proizvode sa makom, sa ciljem da se izvrši analiza opijata u urinu. Jedna osoba je konzumirala štrudlu a druga pletenicu, s tim da je štrudla vizuelno sadržala veću količinu maka. Uzorci urina od obe osobe (po 8 uzoraka) su sakupljeni nakon 5, 12, 24, 30, 36, 48, 60 i 72 sata od konzumacije. Urađena je imunohromatografska i LC/MS analiza na prisustvo opijata. Rezultati imunohromatografske analize su pokazali da je test na opijate (cuttof 300 ng/mL) bio pozitivan u veoma dugom vremenskom periodu, 48 sati nakon konzumacije pletenice i čak 72 sata nakon konzumacije štrudle. LC-MS analizom su potvrđeni rezultati dobijeni preliminarnim testom i u svim pozitivnim uzorcima je dokazano prisustvo glavnih opijatnih alkaloida, morfina i kodeina. Koncentracija morfina je bila veća od koncentracije kodeina u svim uzorcima. Uočena je veća koncentracija morfina (0,25 mg/L) u urinu osobe koja je konzumirala štrudlu u odnosu na koncentraciju morfina (0,06 mg/L) nakon konzumacije pletenice. Rezultati ovog rada potvrđuju da se opijati nalaze u urinu nakon konzumacije proizvoda sa makom i da ih je moguće detektovati čak tokom istog vremenskog perioda kao i kod korisnika psihoaktivnih supstanci, tri dana, i više. Takođe, prema dobijenim rezultatima vreme zadržavanja opijata u urinu je duže nakon konzumacije proizvoda sa većom količinom maka. Navedene rezultate treba imati u vidu prilikom rutinskih testiranja zaposlenih na radnom mestu, pripadnika vojske, učenika u školama i sportista.
PB  - Most Art doo, Beograd
T2  - MD - Medical data
T1  - Analysis of opiates in urine after consumption of products with poppy seed
T1  - Analiza opijata u urinu nakon konzumacije proizvoda sa makom
VL  - 8
IS  - 3
SP  - 207
EP  - 211
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2554
ER  - 

@article{
author = "Baralić, Katarina and Kostadinović, Milka and Antunović, Marko and Đorđević, Snežana and Bulat, Zorica and Ćurčić, Marijana and Đukić-Ćosić, Danijela",
year = "2016",
abstract = "This study presents the case of opiate analysis in urine of two female participants, aged between 20 and 25 who consumed commonly available bakery products with poppy seeds. One of the participants consumed strudel, the product with larger amount of poppy seeds, while the other consumed braid pastry. Urine samples of both participants (n=8) were collected 5, 12, 24, 30, 36, 48, 60 and 72 h after the consummation. Immunochromatographic screening test (cuttof 300 ng/mL) and LC-MS analysis were preformed on the presence of opiates. The screening test for opiates was positive for a very long period of time, 48 h after the consumption of braid pastry and even 72 h after the consumption of strudel. LC-MS analysis verified the results of screening test and the presence of the main opioide alcaloides, morphine and codeine, was confirmed in all positive samples. Determined morphine level was higher than the level of codeine in all samples. The concentration of morphine (0.25 mg/L) was higher in urine of the participant who consumed strudel, compared to the concentration of morphine (0.06 mg/L) after the consumption of braid pastry. The results of this research show that opiates can be found in urine after the consumption of products which contain poppy seed. Morphine and codeine can be detected even during the same time period as in the samples of users of psychoactive substances (3 days and longer). According to the results, retention time of opiates in urine is longer after the consumption of products with higher amounts of poppy. This finding should be taken into account in routine testing of employees in the workplace, members of the military, students in schools, as well as athletes., U ovom radu je prikazan slučaj dve osobe ženskog pola starosti između 20 i 25 godina, koje su konzumirale široko dostupne pekarske proizvode sa makom, sa ciljem da se izvrši analiza opijata u urinu. Jedna osoba je konzumirala štrudlu a druga pletenicu, s tim da je štrudla vizuelno sadržala veću količinu maka. Uzorci urina od obe osobe (po 8 uzoraka) su sakupljeni nakon 5, 12, 24, 30, 36, 48, 60 i 72 sata od konzumacije. Urađena je imunohromatografska i LC/MS analiza na prisustvo opijata. Rezultati imunohromatografske analize su pokazali da je test na opijate (cuttof 300 ng/mL) bio pozitivan u veoma dugom vremenskom periodu, 48 sati nakon konzumacije pletenice i čak 72 sata nakon konzumacije štrudle. LC-MS analizom su potvrđeni rezultati dobijeni preliminarnim testom i u svim pozitivnim uzorcima je dokazano prisustvo glavnih opijatnih alkaloida, morfina i kodeina. Koncentracija morfina je bila veća od koncentracije kodeina u svim uzorcima. Uočena je veća koncentracija morfina (0,25 mg/L) u urinu osobe koja je konzumirala štrudlu u odnosu na koncentraciju morfina (0,06 mg/L) nakon konzumacije pletenice. Rezultati ovog rada potvrđuju da se opijati nalaze u urinu nakon konzumacije proizvoda sa makom i da ih je moguće detektovati čak tokom istog vremenskog perioda kao i kod korisnika psihoaktivnih supstanci, tri dana, i više. Takođe, prema dobijenim rezultatima vreme zadržavanja opijata u urinu je duže nakon konzumacije proizvoda sa većom količinom maka. Navedene rezultate treba imati u vidu prilikom rutinskih testiranja zaposlenih na radnom mestu, pripadnika vojske, učenika u školama i sportista.",
publisher = "Most Art doo, Beograd",
journal = "MD - Medical data",
title = "Analysis of opiates in urine after consumption of products with poppy seed, Analiza opijata u urinu nakon konzumacije proizvoda sa makom",
volume = "8",
number = "3",
pages = "207-211",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2554"
}

Baralić, K., Kostadinović, M., Antunović, M., Đorđević, S., Bulat, Z., Ćurčić, M.,& Đukić-Ćosić, D.. (2016). Analysis of opiates in urine after consumption of products with poppy seed. in MD - Medical data
Most Art doo, Beograd., 8(3), 207-211.
https://hdl.handle.net/21.15107/rcub_farfar_2554

Baralić K, Kostadinović M, Antunović M, Đorđević S, Bulat Z, Ćurčić M, Đukić-Ćosić D. Analysis of opiates in urine after consumption of products with poppy seed. in MD - Medical data. 2016;8(3):207-211.
https://hdl.handle.net/21.15107/rcub_farfar_2554 .

Baralić, Katarina, Kostadinović, Milka, Antunović, Marko, Đorđević, Snežana, Bulat, Zorica, Ćurčić, Marijana, Đukić-Ćosić, Danijela, "Analysis of opiates in urine after consumption of products with poppy seed" in MD - Medical data, 8, no. 3 (2016):207-211,
https://hdl.handle.net/21.15107/rcub_farfar_2554 .

TY  - JOUR
AU  - Ćurčić, Marijana
AU  - Antunović, Marko
AU  - Džoković, Maja
AU  - Janković, Saša
AU  - Milovanović, Vesna
AU  - Đorđević, Snežana
AU  - Kilibarda, Vesna
AU  - Vučinić, Slavica
AU  - Antonijević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2552
AB  - It is known that kidneys are target tissue for Cd toxicity but there is not enough literature data on kidney effects of BDE-209. Moreover, Cd as toxic metal and BDE-209 as organic halogenated pollutant can share similar mechanism of action like, oxidative stress which is already assumed. However, how will Cd act as prooxidant in presence of BDE-209 is still not investigated, therefore, the objective of this study was to assess this BDE-209 influence. Results of this study indicated slight decrease in MDA in rat's kidney homogenates after subacute exposure to Cd and/or BDE-209, while SOD activity was increased and content of -SH groups decreased. Based on dose-response assessment, BDE-209 did not influence Cd toxicity mediated by chosen oxidative stress parameters, namely the derived CEDL5 values were the same for single Cd and for Cd given in combination with the BDE-209. In conclusion, dynamic of oxidative process has influence on the parameters levels. Co-exposure with polybrominated organic pollutant did not influence Cd effects on oxidative stress parameters. Having in mind that this is pilot study, performed with only one dose of BDE-209, further investigations are necessary, including changes in dose ranges, duration of exposure etc. Moreover, results contributes to the issue of mixture toxicology even BDE-209 did not influenced oxidative stress as mechanism of Cd toxicity.
AB  - Poznato je da su bubrezi ciljni organ toksičnosti kadmijuma, ali je mnogo manje literaturnih podataka o toksičnim efektima BDE-209 na bubrege. Kadmijum, kao toksičan metal i BDE-209, kao organska halogenovana zagađujuća supstanca mogu imati sličan mehanizam dejstva posredovan oksidativnim stresom. Međutim, kako će Cd da deluje kao prooksidant u prisustvu BDE-209 još uvek nije proučavano, te je cilj ovog rada bio da se proceni uticaj BDE-209 na oksidativni stres posredovan kadmijumom. Rezultati ovog istraživanja ukazuju na blagi pad koncentracije MDA u homogenatima bubrega pacova nakon subakutne ekspozicije Cd i/ili BDE-209, dok se aktivnost SOD povećava i sadržaj - SH grupa smanjuje. Na osnovu procene odnosa doza-odgovor, BDE-209 nije imao uticaja na toksičnost Cd izazvanu posredstvom oksidativnog stresa (posmatrajući odabrane parametre). Potvrda je dobijena i iz izračunatih donjih granica pouzdanosti doze od 5% - CEDL5 jer su vrednosti su bile iste za sam Cd i Cd primenjen u kombinaciji sa BDE-209. Može se zaključiti da dinamika oksidativnih procesa ima uticaj na vrednosti parametara oksidativnog stresa, da stoga istovremena ekspozicija Cd i polibromovanom organskom polutantu nije promenila uticaj samog Cd na parametre oksidativnog stresa. Imajući u vidu da je ovo pilot studija, izvršena sa samo jednom dozom BDE-209, potrebna su dalja istraživanja, uključujući i promene opsega doza, trajanje ekspozicije itd. Pored toga, rezultati ove studije doprinose tumačenju toksikologija smeša, iako BDE-209 nije imao uticaja na oksidativni stres kao mehanizam Cd toksičnosti za date uslove ekspozicije.
PB  - Most Art doo, Beograd
T2  - MD - Medical data
T1  - Influence of decabrominated diphenyl ether on cadmium as oxidative stress inducer in kidney: Subacute oral study in rats
T1  - Uticaj dekabromovanog difenil etra na toksičnost kadmijuma posredstvom oksidativnog stresa - subakutna oralna studija na pacovima
VL  - 8
IS  - 1
SP  - 17
EP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2552
ER  - 

@article{
author = "Ćurčić, Marijana and Antunović, Marko and Džoković, Maja and Janković, Saša and Milovanović, Vesna and Đorđević, Snežana and Kilibarda, Vesna and Vučinić, Slavica and Antonijević, Biljana",
year = "2016",
abstract = "It is known that kidneys are target tissue for Cd toxicity but there is not enough literature data on kidney effects of BDE-209. Moreover, Cd as toxic metal and BDE-209 as organic halogenated pollutant can share similar mechanism of action like, oxidative stress which is already assumed. However, how will Cd act as prooxidant in presence of BDE-209 is still not investigated, therefore, the objective of this study was to assess this BDE-209 influence. Results of this study indicated slight decrease in MDA in rat's kidney homogenates after subacute exposure to Cd and/or BDE-209, while SOD activity was increased and content of -SH groups decreased. Based on dose-response assessment, BDE-209 did not influence Cd toxicity mediated by chosen oxidative stress parameters, namely the derived CEDL5 values were the same for single Cd and for Cd given in combination with the BDE-209. In conclusion, dynamic of oxidative process has influence on the parameters levels. Co-exposure with polybrominated organic pollutant did not influence Cd effects on oxidative stress parameters. Having in mind that this is pilot study, performed with only one dose of BDE-209, further investigations are necessary, including changes in dose ranges, duration of exposure etc. Moreover, results contributes to the issue of mixture toxicology even BDE-209 did not influenced oxidative stress as mechanism of Cd toxicity., Poznato je da su bubrezi ciljni organ toksičnosti kadmijuma, ali je mnogo manje literaturnih podataka o toksičnim efektima BDE-209 na bubrege. Kadmijum, kao toksičan metal i BDE-209, kao organska halogenovana zagađujuća supstanca mogu imati sličan mehanizam dejstva posredovan oksidativnim stresom. Međutim, kako će Cd da deluje kao prooksidant u prisustvu BDE-209 još uvek nije proučavano, te je cilj ovog rada bio da se proceni uticaj BDE-209 na oksidativni stres posredovan kadmijumom. Rezultati ovog istraživanja ukazuju na blagi pad koncentracije MDA u homogenatima bubrega pacova nakon subakutne ekspozicije Cd i/ili BDE-209, dok se aktivnost SOD povećava i sadržaj - SH grupa smanjuje. Na osnovu procene odnosa doza-odgovor, BDE-209 nije imao uticaja na toksičnost Cd izazvanu posredstvom oksidativnog stresa (posmatrajući odabrane parametre). Potvrda je dobijena i iz izračunatih donjih granica pouzdanosti doze od 5% - CEDL5 jer su vrednosti su bile iste za sam Cd i Cd primenjen u kombinaciji sa BDE-209. Može se zaključiti da dinamika oksidativnih procesa ima uticaj na vrednosti parametara oksidativnog stresa, da stoga istovremena ekspozicija Cd i polibromovanom organskom polutantu nije promenila uticaj samog Cd na parametre oksidativnog stresa. Imajući u vidu da je ovo pilot studija, izvršena sa samo jednom dozom BDE-209, potrebna su dalja istraživanja, uključujući i promene opsega doza, trajanje ekspozicije itd. Pored toga, rezultati ove studije doprinose tumačenju toksikologija smeša, iako BDE-209 nije imao uticaja na oksidativni stres kao mehanizam Cd toksičnosti za date uslove ekspozicije.",
publisher = "Most Art doo, Beograd",
journal = "MD - Medical data",
title = "Influence of decabrominated diphenyl ether on cadmium as oxidative stress inducer in kidney: Subacute oral study in rats, Uticaj dekabromovanog difenil etra na toksičnost kadmijuma posredstvom oksidativnog stresa - subakutna oralna studija na pacovima",
volume = "8",
number = "1",
pages = "17-20",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2552"
}

Ćurčić, M., Antunović, M., Džoković, M., Janković, S., Milovanović, V., Đorđević, S., Kilibarda, V., Vučinić, S.,& Antonijević, B.. (2016). Influence of decabrominated diphenyl ether on cadmium as oxidative stress inducer in kidney: Subacute oral study in rats. in MD - Medical data
Most Art doo, Beograd., 8(1), 17-20.
https://hdl.handle.net/21.15107/rcub_farfar_2552

Ćurčić M, Antunović M, Džoković M, Janković S, Milovanović V, Đorđević S, Kilibarda V, Vučinić S, Antonijević B. Influence of decabrominated diphenyl ether on cadmium as oxidative stress inducer in kidney: Subacute oral study in rats. in MD - Medical data. 2016;8(1):17-20.
https://hdl.handle.net/21.15107/rcub_farfar_2552 .

Ćurčić, Marijana, Antunović, Marko, Džoković, Maja, Janković, Saša, Milovanović, Vesna, Đorđević, Snežana, Kilibarda, Vesna, Vučinić, Slavica, Antonijević, Biljana, "Influence of decabrominated diphenyl ether on cadmium as oxidative stress inducer in kidney: Subacute oral study in rats" in MD - Medical data, 8, no. 1 (2016):17-20,
https://hdl.handle.net/21.15107/rcub_farfar_2552 .

TY  - JOUR
AU  - Sulić, Krsta
AU  - Đorđević, Snežana
AU  - Rusić, Branislava
AU  - Denić, Kristina
AU  - Nešić, Vladimir
AU  - Antunović, Marko
AU  - Ćurčić, Marijana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2122
AB  - Detection of most abusing drug - marijuana in urine speciments is performed by immunochormatographic tests. However, each positive test result has to be confirmed by mass spectrometric detection after analyzing samples with some of chromatographic techniques. We developed liquid chromatography-mass spectrometric (LC-MS) method for determination of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THCA) as a major THC metabolite in urine. Because of low concentration of free THCA in urine, it has to perform hydrolysis of its glucuronide. Hydrolysis of glucuronide from urine samples was performed with KOH on 60°C. THCA and internal standard (THCA-D3) were extracted from urine after adjusting of pH on 3 with mixture n-hexan-ethylacetate (7:1). THCA was determined by LC-MS method in single ion monitoring mode (SIM) at m/z 345, 327 and 299 for THCA, and 348, 330 and 302 for IS. The chromatographic separation was performed on XTerra®RP18 column, using a gradient of acetonitrile/acetic acid 1% and acetate buffer pH 3.5 as the mobile phase. Linearity was achieved in the range from 10-100 ng/mL. Retention times of THCA and IS were 21.54 and 21.52 minutes respectively. Limit of detection and limit of quantitation for THCA were 2.65 and 8.82 ng/mL respectively. Coefficient of variation was 7.62%. Described LC-MS method of analysis THCA in urine is precise, accurate, reproducible and reliable method for confirmation of cannabis abuse.
AB  - Detekcija najčešće zloupotrebljavane psihoaktivne supstance - marihuane u urinu vrši se imunohromatografskim testom. Međutim, svaki pozitivan rezultat mora da bude potvrđen maseno-spektrometrijskom metodom nakon analize uzorka nekom od hromatografskih tehnika. Mi smo razvili metodu tečne hromatografije sa masenom spektrometrijom za određivanje 11-nor-Δ9-tetrahidrokanabinol-9-karboksilne kiseline (THCA) kao glavnog metabolita THC u urinu. Zbog malih koncentracija slobodne THCA u urinu, potrebno je uraditi hidrolizu glukuronida. Hirdoliza glukuronida iz uzorka urina vršena je pomoću KOH na 60°C. THCA i interni standard THCA-D3 su izolovani iz urina pomoću smeše n-heksan-etilacetat (7:1) posle podešavanja pH na 3. THCA je određivana LC-MS metodom u SIM modu sa jonskim masama m/z 345, 327 i 299 za THCA i 348, 330 i 302 za IS. Linearnost je postignuta u opsegu koncentracija od 10-100 ng/mL. Retenciona vremena za THCA i IS su bila 21,54 i 21,52 min. Limit detekcije i kvantifikacije za THCA bili su 2,65 i 8,82 ng/mL. Koeficijent varijacije je iznosio 7,62%. Prikazana LC-MS metoda za određivanje THCA u urinu je precizna, tačna, ponovljiva i pouzdana metoda za potvrdu zloupotrebe kanabisa.
PB  - Most Art doo, Beograd
T2  - MD - Medical data
T1  - Determination of tetrahydrocannabinol-carboxylic acid in urine by liquid chromatography with mass spectrometry
T1  - Određivanje tetrahidrokanabinol--karboksilne kiseline u urinu primenom tečne hromatografije sa masenom-spektrometrijom
VL  - 6
IS  - 1
SP  - 51
EP  - 54
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2122
ER  - 

@article{
author = "Sulić, Krsta and Đorđević, Snežana and Rusić, Branislava and Denić, Kristina and Nešić, Vladimir and Antunović, Marko and Ćurčić, Marijana",
year = "2014",
abstract = "Detection of most abusing drug - marijuana in urine speciments is performed by immunochormatographic tests. However, each positive test result has to be confirmed by mass spectrometric detection after analyzing samples with some of chromatographic techniques. We developed liquid chromatography-mass spectrometric (LC-MS) method for determination of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THCA) as a major THC metabolite in urine. Because of low concentration of free THCA in urine, it has to perform hydrolysis of its glucuronide. Hydrolysis of glucuronide from urine samples was performed with KOH on 60°C. THCA and internal standard (THCA-D3) were extracted from urine after adjusting of pH on 3 with mixture n-hexan-ethylacetate (7:1). THCA was determined by LC-MS method in single ion monitoring mode (SIM) at m/z 345, 327 and 299 for THCA, and 348, 330 and 302 for IS. The chromatographic separation was performed on XTerra®RP18 column, using a gradient of acetonitrile/acetic acid 1% and acetate buffer pH 3.5 as the mobile phase. Linearity was achieved in the range from 10-100 ng/mL. Retention times of THCA and IS were 21.54 and 21.52 minutes respectively. Limit of detection and limit of quantitation for THCA were 2.65 and 8.82 ng/mL respectively. Coefficient of variation was 7.62%. Described LC-MS method of analysis THCA in urine is precise, accurate, reproducible and reliable method for confirmation of cannabis abuse., Detekcija najčešće zloupotrebljavane psihoaktivne supstance - marihuane u urinu vrši se imunohromatografskim testom. Međutim, svaki pozitivan rezultat mora da bude potvrđen maseno-spektrometrijskom metodom nakon analize uzorka nekom od hromatografskih tehnika. Mi smo razvili metodu tečne hromatografije sa masenom spektrometrijom za određivanje 11-nor-Δ9-tetrahidrokanabinol-9-karboksilne kiseline (THCA) kao glavnog metabolita THC u urinu. Zbog malih koncentracija slobodne THCA u urinu, potrebno je uraditi hidrolizu glukuronida. Hirdoliza glukuronida iz uzorka urina vršena je pomoću KOH na 60°C. THCA i interni standard THCA-D3 su izolovani iz urina pomoću smeše n-heksan-etilacetat (7:1) posle podešavanja pH na 3. THCA je određivana LC-MS metodom u SIM modu sa jonskim masama m/z 345, 327 i 299 za THCA i 348, 330 i 302 za IS. Linearnost je postignuta u opsegu koncentracija od 10-100 ng/mL. Retenciona vremena za THCA i IS su bila 21,54 i 21,52 min. Limit detekcije i kvantifikacije za THCA bili su 2,65 i 8,82 ng/mL. Koeficijent varijacije je iznosio 7,62%. Prikazana LC-MS metoda za određivanje THCA u urinu je precizna, tačna, ponovljiva i pouzdana metoda za potvrdu zloupotrebe kanabisa.",
publisher = "Most Art doo, Beograd",
journal = "MD - Medical data",
title = "Determination of tetrahydrocannabinol-carboxylic acid in urine by liquid chromatography with mass spectrometry, Određivanje tetrahidrokanabinol--karboksilne kiseline u urinu primenom tečne hromatografije sa masenom-spektrometrijom",
volume = "6",
number = "1",
pages = "51-54",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2122"
}

Sulić, K., Đorđević, S., Rusić, B., Denić, K., Nešić, V., Antunović, M.,& Ćurčić, M.. (2014). Determination of tetrahydrocannabinol-carboxylic acid in urine by liquid chromatography with mass spectrometry. in MD - Medical data
Most Art doo, Beograd., 6(1), 51-54.
https://hdl.handle.net/21.15107/rcub_farfar_2122

Sulić K, Đorđević S, Rusić B, Denić K, Nešić V, Antunović M, Ćurčić M. Determination of tetrahydrocannabinol-carboxylic acid in urine by liquid chromatography with mass spectrometry. in MD - Medical data. 2014;6(1):51-54.
https://hdl.handle.net/21.15107/rcub_farfar_2122 .

Sulić, Krsta, Đorđević, Snežana, Rusić, Branislava, Denić, Kristina, Nešić, Vladimir, Antunović, Marko, Ćurčić, Marijana, "Determination of tetrahydrocannabinol-carboxylic acid in urine by liquid chromatography with mass spectrometry" in MD - Medical data, 6, no. 1 (2014):51-54,
https://hdl.handle.net/21.15107/rcub_farfar_2122 .