@conference{
author = "Atanacković, Milica and Baralić, Katarina and Ćurčić, Marijana and Javorac, Dragana and Buha-Đorđević, Aleksandra and Antonijević-Miljaković, Evica and Antonijević, Biljana and Bulat, Zorica and Đukić-Ćosić, Danijela",
year = "2022",
abstract = "Opioids are a wide group of natural, semi-synthetic and synthetic substances, used in
the pharmacotherapy of painful conditions. However, due to their psychoactive properties,
they are often abused. Immunochromatographic test panels are used for substance abuse
monitoring and are quick, simple and based on the principle of competitive binding. Main
disadvantage of immunochromatographic analysis is cross-reactivity, because of sufficient
specificity of test antibodies for the analyte of interest. This study aimed to examine the
effect of codeine-based OTC preparation (Caffetin®) on false positive results of preliminary
opiate urineanalysis, and determine the time period during which these results may occur.
The study included two female healthy subjects, 23 and 24 years old. One of them
administered double oral dose of Caffetin® (two tablets - total of 20 mg of codeine) and the
other administred single oral dose of Caffetin® (one tablet - 10mg of codeine). Urine samples
were collected for 48h. Analysis was performed by AmeritekOPI® monocomponent test
strips and DOAMultiGnost 3 Test Panel® multicomponent test strips. The use of Caffetin®
gave false positive results for opiates after 4,7h (Subject 1) and 3,3 h (Subject 2). The first
negative result occurred after 39h (Subject 1) and 37,9h (Subject 2). After application of
codeine-based OTC preparations, false positive results were obtained in less than 48h.
Literature data indicate that positive results on opiates might occur 48h after consuming of
codeine-based medication. Hence, it is necessary to carry out further research with aim to
avoid doubts during interpretation of immunochromatographic test results., Opioidi predstavljaju široku grupu supstanci prirodnog, polusintetskog i sintetskog
porekla, koje se osim primene u farmakoterapiji bolnih stanja, , često zloupotrebljavaju. Za
kontrolu zloupotrebe koriste se imunohromatografske test trake, brza i jednostavna analiza
zasnovana na principu kompetitivnog vezivanja. Glavni nedostatak im je pojava ukrštene
reaktivnosti usled niske specifičnosti antitela za analit od interesa. Cilj ovog rada je bio
ispitati uticaj primene OTC preparata koji sadrži kodein (Caffetin®) na pojavu lažno
pozitivnih rezultata preliminarne imunohromatografske analize opijata u urinu i utvrditi
vremenski period njihove pojave od trenutka primene analgetika. U istraživanju su
učestvovale dve zdrave ženske osobe starosti 23 i 24 godine. Jedna ispitanica je primenila
dozu koja sadrži 20 mg kodeina (2 tablete), a druga 10 mg kodeina (1 tableta). Uzorci urina
su sakupljani tokom narednih 48 h i analiza je vršena pomoću dve vrste
imunohromatografskih test traka: monokomponentnim AmeritekOPI ® i trokomponentnim
DOAMultiGnost 3 Test Panel® . Primenom Caffetin® tableta dobijeni su lažno pozitivni
rezultati na opijate prvi put nakon 4,7 h (Ispitanica 1) i 3,3 h (Ispitanica 2), a prvi negativan
rezultat se javio posle 39 h (Ispitanica 1) i 37,9 h (Ispitanica 2). Utvrđeno je da se nakon
primene OTC preparata sa kodeinom dobijaju lažno pozitivni rezultati na opijate u
vremenskom periodu kraćem od 48 h. Imajući u vidu da literaturni podaci ukazuju na to da
uzorak može biti pozitivan na opijate i do 48 h pri primeni kodeina, neophodno je sprovesti
detaljnija ispitivanja kako bi ova pojava u manjoj meri dovodila do nedoumica prilikom
tumačenju rezultata imunohromatografskih testova.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Time-dependent occurrence of false-positive results of opiate test strips after CAFFETIN® use, Vremenski zavisna pojava lažno pozitivnih rezultata test traka na opijate nakon primene leka CAFFETIN®",
volume = "72",
number = "4 suplement",
pages = "S599-S600",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4636"
}
