TY  - JOUR
AU  - Joksić, Gordana
AU  - Micić, Mileva
AU  - Filipović, Jelena
AU  - Drakulić, Dunja
AU  - Stanojlović, Miloš
AU  - Čalija, Bojan
AU  - Valenta Sobot, Ana
AU  - Demajo, Miroslav
AU  - Nilsson, Robert
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3015
AB  - The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2'deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2'-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2'-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2'-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2'-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2'-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2'-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach
VL  - 67
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1515/acve-2017-0001
ER  - 

@article{
author = "Joksić, Gordana and Micić, Mileva and Filipović, Jelena and Drakulić, Dunja and Stanojlović, Miloš and Čalija, Bojan and Valenta Sobot, Ana and Demajo, Miroslav and Nilsson, Robert",
year = "2017",
abstract = "The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2'deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2'-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2'-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2'-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2'-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2'-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2'-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach",
volume = "67",
number = "1",
pages = "1-10",
doi = "10.1515/acve-2017-0001"
}

Joksić, G., Micić, M., Filipović, J., Drakulić, D., Stanojlović, M., Čalija, B., Valenta Sobot, A., Demajo, M.,& Nilsson, R.. (2017). Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(1), 1-10.
https://doi.org/10.1515/acve-2017-0001

Joksić G, Micić M, Filipović J, Drakulić D, Stanojlović M, Čalija B, Valenta Sobot A, Demajo M, Nilsson R. Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach. in Acta veterinaria. 2017;67(1):1-10.
doi:10.1515/acve-2017-0001 .

Joksić, Gordana, Micić, Mileva, Filipović, Jelena, Drakulić, Dunja, Stanojlović, Miloš, Čalija, Bojan, Valenta Sobot, Ana, Demajo, Miroslav, Nilsson, Robert, "Cell proliferation assay - Method optimisation for in vivo labeling of DNA in the rat forestomach" in Acta veterinaria, 67, no. 1 (2017):1-10,
https://doi.org/10.1515/acve-2017-0001 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Stanković, M
AU  - Vasić, Vesna
AU  - Čakar, Mira
AU  - Jokanović, M
PY  - 2000
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/243
AB  - Cytotoxic effects of the antiviremic drug ribavirin (1-beta -D-ribofuranosyl-1,2,4-triazole-3-carboxamide) were evaluated in vitro measuring micronucleus formation and cell proliferation kinetics in whole blood cultures employing cytokinesis block (CB) micronucleus test. The cells were exposed to ribavirin doses ranging from 0.05-0.65 mu mol/ml at three different incubation times. The frequency of micronuclei in treated samples demonstrated relatively low ability of ribavirin to induce micronuclei. However, the lowest concentration of ribavirin markedly changed the frequency of mononucleated and multinucleated cells, particularly binucleated ones, which declined significantly. The decline in the frequency of binucleate cells was followed with accumulation of greater number of mononucleate cells. Decreased proliferation potential of lymphocytes treated with ribavirin indicates that cells are arrested prior to metaphase. The present investigation showed that ribavirin is capable to induce a delay in cellular proliferation at all the doses assayed. The study demonstrated that CB micronucleus assay is simple and rapid method that can be used to assess toxic effect of drugs in vitro.
PB  - Slovak Academic Press Ltd, Bratislava
T2  - Neoplasma
T1  - Influence of ribavirin on the micronucleus formation and in vitro proliferation of human lymphocytes
VL  - 47
IS  - 5
SP  - 283
EP  - 287
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2383
ER  - 

@article{
author = "Joksić, Gordana and Stanković, M and Vasić, Vesna and Čakar, Mira and Jokanović, M",
year = "2000",
abstract = "Cytotoxic effects of the antiviremic drug ribavirin (1-beta -D-ribofuranosyl-1,2,4-triazole-3-carboxamide) were evaluated in vitro measuring micronucleus formation and cell proliferation kinetics in whole blood cultures employing cytokinesis block (CB) micronucleus test. The cells were exposed to ribavirin doses ranging from 0.05-0.65 mu mol/ml at three different incubation times. The frequency of micronuclei in treated samples demonstrated relatively low ability of ribavirin to induce micronuclei. However, the lowest concentration of ribavirin markedly changed the frequency of mononucleated and multinucleated cells, particularly binucleated ones, which declined significantly. The decline in the frequency of binucleate cells was followed with accumulation of greater number of mononucleate cells. Decreased proliferation potential of lymphocytes treated with ribavirin indicates that cells are arrested prior to metaphase. The present investigation showed that ribavirin is capable to induce a delay in cellular proliferation at all the doses assayed. The study demonstrated that CB micronucleus assay is simple and rapid method that can be used to assess toxic effect of drugs in vitro.",
publisher = "Slovak Academic Press Ltd, Bratislava",
journal = "Neoplasma",
title = "Influence of ribavirin on the micronucleus formation and in vitro proliferation of human lymphocytes",
volume = "47",
number = "5",
pages = "283-287",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2383"
}

Joksić, G., Stanković, M., Vasić, V., Čakar, M.,& Jokanović, M.. (2000). Influence of ribavirin on the micronucleus formation and in vitro proliferation of human lymphocytes. in Neoplasma
Slovak Academic Press Ltd, Bratislava., 47(5), 283-287.
https://hdl.handle.net/21.15107/rcub_vinar_2383

Joksić G, Stanković M, Vasić V, Čakar M, Jokanović M. Influence of ribavirin on the micronucleus formation and in vitro proliferation of human lymphocytes. in Neoplasma. 2000;47(5):283-287.
https://hdl.handle.net/21.15107/rcub_vinar_2383 .

Joksić, Gordana, Stanković, M, Vasić, Vesna, Čakar, Mira, Jokanović, M, "Influence of ribavirin on the micronucleus formation and in vitro proliferation of human lymphocytes" in Neoplasma, 47, no. 5 (2000):283-287,
https://hdl.handle.net/21.15107/rcub_vinar_2383 .