TY  - JOUR
AU  - Milinković, Neda
AU  - Ignjatović, Svetlana
AU  - Žarković, Miloš
AU  - Jovičić, Snežana
AU  - Radosavljević, Branimir
AU  - Singh, Sandra
AU  - Majkić-Singh, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2099
AB  - Objectives. Defining adequate reference limits (RLs) for thyroid hormones is an important task for support monitoring and the treatment of subclinical thyroid disease. We determined whether there are age-related RLs for thyroid parameters in male and female outpatients free of overt thyroid disease. Design. We analyzed 22,860 results (11,440 male and 11,420 female outpatients above the age of 18) for thyrotropin (TSH), free thyroxine (fT4) and total triiodothyronine (T3) that were stored in our laboratory information system between 2008 and 2011. We calculated the 2.5th and 97.5th centiles for the analyzed thyroid parameters. Results. Our results indicate higher TSH levels with ageing, with a significant difference (p  lt  0.05) between the 97.5th centiles for males and females older than 70 (5.07 mIU/L and 4.10 mIU/L), but also a significant difference between male and female fT4 from 31 to 40 and from 41 to 50 years old (18.4 vs 14.9 pmol/L and 19.0 vs 15.9 pmol/L, p  lt  0.05), respectively. Overall indirect estimates of the 97.5th centiles for TSH for males and females were not significantly different and were below the generally recommended upper limit (4.01 mIU/L and 4.20 mIU/L, respectively). In addition, we found no statistically signifi cant change in mean T3 values in the analyzed population. Conclusions. This cross-sectional study indicates change in TSH and fT4 levels with ageing and gender-related upper limits. This suggests that by using indirect estimation a laboratory could provide clinicians with more accurate gender- and age-specific RLs for thyroid parameters.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Scandinavian Journal of Clinical and Laboratory Investigation
T1  - Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results
VL  - 74
IS  - 5
SP  - 378
EP  - 384
DO  - 10.3109/00365513.2014.898324
ER  - 

@article{
author = "Milinković, Neda and Ignjatović, Svetlana and Žarković, Miloš and Jovičić, Snežana and Radosavljević, Branimir and Singh, Sandra and Majkić-Singh, Nada",
year = "2014",
abstract = "Objectives. Defining adequate reference limits (RLs) for thyroid hormones is an important task for support monitoring and the treatment of subclinical thyroid disease. We determined whether there are age-related RLs for thyroid parameters in male and female outpatients free of overt thyroid disease. Design. We analyzed 22,860 results (11,440 male and 11,420 female outpatients above the age of 18) for thyrotropin (TSH), free thyroxine (fT4) and total triiodothyronine (T3) that were stored in our laboratory information system between 2008 and 2011. We calculated the 2.5th and 97.5th centiles for the analyzed thyroid parameters. Results. Our results indicate higher TSH levels with ageing, with a significant difference (p  lt  0.05) between the 97.5th centiles for males and females older than 70 (5.07 mIU/L and 4.10 mIU/L), but also a significant difference between male and female fT4 from 31 to 40 and from 41 to 50 years old (18.4 vs 14.9 pmol/L and 19.0 vs 15.9 pmol/L, p  lt  0.05), respectively. Overall indirect estimates of the 97.5th centiles for TSH for males and females were not significantly different and were below the generally recommended upper limit (4.01 mIU/L and 4.20 mIU/L, respectively). In addition, we found no statistically signifi cant change in mean T3 values in the analyzed population. Conclusions. This cross-sectional study indicates change in TSH and fT4 levels with ageing and gender-related upper limits. This suggests that by using indirect estimation a laboratory could provide clinicians with more accurate gender- and age-specific RLs for thyroid parameters.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Scandinavian Journal of Clinical and Laboratory Investigation",
title = "Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results",
volume = "74",
number = "5",
pages = "378-384",
doi = "10.3109/00365513.2014.898324"
}

Milinković, N., Ignjatović, S., Žarković, M., Jovičić, S., Radosavljević, B., Singh, S.,& Majkić-Singh, N.. (2014). Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results. in Scandinavian Journal of Clinical and Laboratory Investigation
Taylor & Francis Ltd, Abingdon., 74(5), 378-384.
https://doi.org/10.3109/00365513.2014.898324

Milinković N, Ignjatović S, Žarković M, Jovičić S, Radosavljević B, Singh S, Majkić-Singh N. Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results. in Scandinavian Journal of Clinical and Laboratory Investigation. 2014;74(5):378-384.
doi:10.3109/00365513.2014.898324 .

Milinković, Neda, Ignjatović, Svetlana, Žarković, Miloš, Jovičić, Snežana, Radosavljević, Branimir, Singh, Sandra, Majkić-Singh, Nada, "Indirect estimation of age-related reference limits of thyroid parameters: A cross-sectional study of outpatients' results" in Scandinavian Journal of Clinical and Laboratory Investigation, 74, no. 5 (2014):378-384,
https://doi.org/10.3109/00365513.2014.898324 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Seferović, Jelena
AU  - Ignjatović, Svetlana
AU  - Singh, Sandra
AU  - Majkić-Singh, Nada
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1756
AB  - The importance of the association of antiphospholipid antibodies (aPL Abs) with the features of type II diabetes mellitus has not yet been elucidated. The aim of this work was to investigate the association of aPL Abs with adiponectin and non-esterified fatty acids (NEFA) in type II diabetes mellitus patients without micro and/or macrovascular complications, and to analyze the differences between the male and female patients with regard to the abovementioned parameters. Male patients with type II diabetes mellitus showed a positive correlation between NEFA concentrations and anti-oxLDL antibodies (r = 0.334, p = 0.019). A weak, but statistically significant correlation between adiponectin concentrations and the IgM isotype of anti-annexin A5 antibodies was found in type II diabetes mellitus patients (r = 0.285, p = 0.011). The presence of a positive correlation between NEFA and anti-oxLDL antibodies might be useful in the detection of patients with premature atherosclerosis in type II diabetes mellitus patients without any micro and/or macrovascular complications among type II diabetes mellitus patients.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus
VL  - 31
IS  - 3
SP  - 199
EP  - 204
DO  - 10.2478/v10011-012-0009-y
ER  - 

@article{
author = "Becarević, Mirjana and Seferović, Jelena and Ignjatović, Svetlana and Singh, Sandra and Majkić-Singh, Nada",
year = "2012",
abstract = "The importance of the association of antiphospholipid antibodies (aPL Abs) with the features of type II diabetes mellitus has not yet been elucidated. The aim of this work was to investigate the association of aPL Abs with adiponectin and non-esterified fatty acids (NEFA) in type II diabetes mellitus patients without micro and/or macrovascular complications, and to analyze the differences between the male and female patients with regard to the abovementioned parameters. Male patients with type II diabetes mellitus showed a positive correlation between NEFA concentrations and anti-oxLDL antibodies (r = 0.334, p = 0.019). A weak, but statistically significant correlation between adiponectin concentrations and the IgM isotype of anti-annexin A5 antibodies was found in type II diabetes mellitus patients (r = 0.285, p = 0.011). The presence of a positive correlation between NEFA and anti-oxLDL antibodies might be useful in the detection of patients with premature atherosclerosis in type II diabetes mellitus patients without any micro and/or macrovascular complications among type II diabetes mellitus patients.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus",
volume = "31",
number = "3",
pages = "199-204",
doi = "10.2478/v10011-012-0009-y"
}

Becarević, M., Seferović, J., Ignjatović, S., Singh, S.,& Majkić-Singh, N.. (2012). Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 31(3), 199-204.
https://doi.org/10.2478/v10011-012-0009-y

Becarević M, Seferović J, Ignjatović S, Singh S, Majkić-Singh N. Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus. in Journal of Medical Biochemistry. 2012;31(3):199-204.
doi:10.2478/v10011-012-0009-y .

Becarević, Mirjana, Seferović, Jelena, Ignjatović, Svetlana, Singh, Sandra, Majkić-Singh, Nada, "Adiponectin, non-esterified fatty acids and antiphospholipid antibodies in type II diabetes mellitus" in Journal of Medical Biochemistry, 31, no. 3 (2012):199-204,
https://doi.org/10.2478/v10011-012-0009-y . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Seferović, Jelena
AU  - Ignjatović, Svetlana
AU  - Singh, Sandra
AU  - Majkić-Singh, Nada
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1552
AB  - Objective: In vitro, stimulation of monocytes with antiphospholipid (aPL) antibodies resulted in increased secretion of TNF-alpha, but association of aPL with features of diabetes mellitus is not clarified yet. Therefore, we investigated the distribution of anticardiolipin (aCL), anti-beta 2gpI (a beta 2gpI), anti-annexin A5 (aannxA5), and anti-oxLDL (aoxLDL) antibodies, and TNF-alpha in well-formed group of 78 patients with type II diabetes mellitus without vascular complications. Methods: Investigated antibodies and TNF-alpha concentrations were measured by ELISA. Results: Antiphospholipid antibodies were in positive correlation with TNF-alpha concentrations: aCL IgG (r = 0.303, p = 0.007), aCL IgM (r = 0.386, p = 0.000), a beta 2gpI IgG (r = 0.499, p = 0.000), a beta 2gpI IgM (r = 0.462, p = 0.000), aanxA5 IgG (r = 0.479, p = 0.000), aanxA5 IgM (r = 0.641, p = 0.000), aoxLDL (IgG + IgM, r = 0.279, p = 0.000). Anticardiolipin-positive and aCL-negative subgroups differed in TNF-alpha concentrations (Mann-Whitney, p = 0.032). Significantly elevated LDL concentrations were noticed in aCL-positive patients with disease duration 10-15 years (chi(2) = 15.000, p = 0.000) and apoB concentrations were elevated in aoxLDL-positive patients with disease duration 7-10 years (chi(2) = 3.938, p = 0.047). Conclusion: Significant association of antiphospholipid antibodies and TNF-alpha might be a marker of severe atherogenic profile (suggested by increased levels of lipids in aPL-positive subgroups) and should be used for the stratification of patients with an increased risk for future deterioration of the disease.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Cytokine
T1  - Significant association of antiphospholipid antibodies and TNF-alpha: Marker of severe atherogenic profile of patients with type II diabetes mellitus without micro and/or macrovascular complications
VL  - 55
IS  - 2
SP  - 301
EP  - 306
DO  - 10.1016/j.cyto.2011.05.001
ER  - 

@article{
author = "Becarević, Mirjana and Seferović, Jelena and Ignjatović, Svetlana and Singh, Sandra and Majkić-Singh, Nada",
year = "2011",
abstract = "Objective: In vitro, stimulation of monocytes with antiphospholipid (aPL) antibodies resulted in increased secretion of TNF-alpha, but association of aPL with features of diabetes mellitus is not clarified yet. Therefore, we investigated the distribution of anticardiolipin (aCL), anti-beta 2gpI (a beta 2gpI), anti-annexin A5 (aannxA5), and anti-oxLDL (aoxLDL) antibodies, and TNF-alpha in well-formed group of 78 patients with type II diabetes mellitus without vascular complications. Methods: Investigated antibodies and TNF-alpha concentrations were measured by ELISA. Results: Antiphospholipid antibodies were in positive correlation with TNF-alpha concentrations: aCL IgG (r = 0.303, p = 0.007), aCL IgM (r = 0.386, p = 0.000), a beta 2gpI IgG (r = 0.499, p = 0.000), a beta 2gpI IgM (r = 0.462, p = 0.000), aanxA5 IgG (r = 0.479, p = 0.000), aanxA5 IgM (r = 0.641, p = 0.000), aoxLDL (IgG + IgM, r = 0.279, p = 0.000). Anticardiolipin-positive and aCL-negative subgroups differed in TNF-alpha concentrations (Mann-Whitney, p = 0.032). Significantly elevated LDL concentrations were noticed in aCL-positive patients with disease duration 10-15 years (chi(2) = 15.000, p = 0.000) and apoB concentrations were elevated in aoxLDL-positive patients with disease duration 7-10 years (chi(2) = 3.938, p = 0.047). Conclusion: Significant association of antiphospholipid antibodies and TNF-alpha might be a marker of severe atherogenic profile (suggested by increased levels of lipids in aPL-positive subgroups) and should be used for the stratification of patients with an increased risk for future deterioration of the disease.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Cytokine",
title = "Significant association of antiphospholipid antibodies and TNF-alpha: Marker of severe atherogenic profile of patients with type II diabetes mellitus without micro and/or macrovascular complications",
volume = "55",
number = "2",
pages = "301-306",
doi = "10.1016/j.cyto.2011.05.001"
}

Becarević, M., Seferović, J., Ignjatović, S., Singh, S.,& Majkić-Singh, N.. (2011). Significant association of antiphospholipid antibodies and TNF-alpha: Marker of severe atherogenic profile of patients with type II diabetes mellitus without micro and/or macrovascular complications. in Cytokine
Academic Press Ltd- Elsevier Science Ltd, London., 55(2), 301-306.
https://doi.org/10.1016/j.cyto.2011.05.001

Becarević M, Seferović J, Ignjatović S, Singh S, Majkić-Singh N. Significant association of antiphospholipid antibodies and TNF-alpha: Marker of severe atherogenic profile of patients with type II diabetes mellitus without micro and/or macrovascular complications. in Cytokine. 2011;55(2):301-306.
doi:10.1016/j.cyto.2011.05.001 .

Becarević, Mirjana, Seferović, Jelena, Ignjatović, Svetlana, Singh, Sandra, Majkić-Singh, Nada, "Significant association of antiphospholipid antibodies and TNF-alpha: Marker of severe atherogenic profile of patients with type II diabetes mellitus without micro and/or macrovascular complications" in Cytokine, 55, no. 2 (2011):301-306,
https://doi.org/10.1016/j.cyto.2011.05.001 . .

TY  - CONF
AU  - Lalić, K.
AU  - Zamaklar, M.
AU  - Lalić, N. M.
AU  - Ostojić, Miodrag
AU  - Kalimanovska, Vesna
AU  - Jotić, A.
AU  - Rajković, N.
AU  - Lukić, L.
AU  - Milicić, T.
AU  - Singh, Sandra
AU  - Stosić, L.
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1257
PB  - Springer, New York
C3  - Diabetologia
T1  - Hypertriglyceridaemic waist phenotype is associated with increased severity of coronary artery disease both in type 2 diabetes and nondiabetic patients
VL  - 52
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1257
ER  - 

@conference{
author = "Lalić, K. and Zamaklar, M. and Lalić, N. M. and Ostojić, Miodrag and Kalimanovska, Vesna and Jotić, A. and Rajković, N. and Lukić, L. and Milicić, T. and Singh, Sandra and Stosić, L.",
year = "2009",
publisher = "Springer, New York",
journal = "Diabetologia",
title = "Hypertriglyceridaemic waist phenotype is associated with increased severity of coronary artery disease both in type 2 diabetes and nondiabetic patients",
volume = "52",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1257"
}

Lalić, K., Zamaklar, M., Lalić, N. M., Ostojić, M., Kalimanovska, V., Jotić, A., Rajković, N., Lukić, L., Milicić, T., Singh, S.,& Stosić, L.. (2009). Hypertriglyceridaemic waist phenotype is associated with increased severity of coronary artery disease both in type 2 diabetes and nondiabetic patients. in Diabetologia
Springer, New York., 52.
https://hdl.handle.net/21.15107/rcub_farfar_1257

Lalić K, Zamaklar M, Lalić NM, Ostojić M, Kalimanovska V, Jotić A, Rajković N, Lukić L, Milicić T, Singh S, Stosić L. Hypertriglyceridaemic waist phenotype is associated with increased severity of coronary artery disease both in type 2 diabetes and nondiabetic patients. in Diabetologia. 2009;52.
https://hdl.handle.net/21.15107/rcub_farfar_1257 .

Lalić, K., Zamaklar, M., Lalić, N. M., Ostojić, Miodrag, Kalimanovska, Vesna, Jotić, A., Rajković, N., Lukić, L., Milicić, T., Singh, Sandra, Stosić, L., "Hypertriglyceridaemic waist phenotype is associated with increased severity of coronary artery disease both in type 2 diabetes and nondiabetic patients" in Diabetologia, 52 (2009),
https://hdl.handle.net/21.15107/rcub_farfar_1257 .