TY  - CONF
AU  - Petrović, Jelena
AU  - Labudović-Borović, Milica
AU  - Puškaš, Nela
AU  - Stanić, Dušanka
AU  - Batinić, Bojan
AU  - Plećaš-Solarović, Bosiljka
AU  - Pešić, Vesna
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2894
PB  - Elsevier Science BV, Amsterdam
C3  - European Neuropsychopharmacology
T1  - Chronic magnesium supplementation increases hippocampal neurogenesis and decreases proliferation in myocardium in ACTH-treated rats
VL  - 27
IS  - Supplement 4
SP  - S765
EP  - S766
DO  - 10.1016/S0924-977X(17)31398-6
ER  - 

@conference{
author = "Petrović, Jelena and Labudović-Borović, Milica and Puškaš, Nela and Stanić, Dušanka and Batinić, Bojan and Plećaš-Solarović, Bosiljka and Pešić, Vesna",
year = "2017",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Neuropsychopharmacology",
title = "Chronic magnesium supplementation increases hippocampal neurogenesis and decreases proliferation in myocardium in ACTH-treated rats",
volume = "27",
number = "Supplement 4",
pages = "S765-S766",
doi = "10.1016/S0924-977X(17)31398-6"
}

Petrović, J., Labudović-Borović, M., Puškaš, N., Stanić, D., Batinić, B., Plećaš-Solarović, B.,& Pešić, V.. (2017). Chronic magnesium supplementation increases hippocampal neurogenesis and decreases proliferation in myocardium in ACTH-treated rats. in European Neuropsychopharmacology
Elsevier Science BV, Amsterdam., 27(Supplement 4), S765-S766.
https://doi.org/10.1016/S0924-977X(17)31398-6

Petrović J, Labudović-Borović M, Puškaš N, Stanić D, Batinić B, Plećaš-Solarović B, Pešić V. Chronic magnesium supplementation increases hippocampal neurogenesis and decreases proliferation in myocardium in ACTH-treated rats. in European Neuropsychopharmacology. 2017;27(Supplement 4):S765-S766.
doi:10.1016/S0924-977X(17)31398-6 .

Petrović, Jelena, Labudović-Borović, Milica, Puškaš, Nela, Stanić, Dušanka, Batinić, Bojan, Plećaš-Solarović, Bosiljka, Pešić, Vesna, "Chronic magnesium supplementation increases hippocampal neurogenesis and decreases proliferation in myocardium in ACTH-treated rats" in European Neuropsychopharmacology, 27, no. Supplement 4 (2017):S765-S766,
https://doi.org/10.1016/S0924-977X(17)31398-6 . .

TY  - JOUR
AU  - Stanić, Dušanka
AU  - Plećaš-Solarović, Bosiljka
AU  - Mirković, Duško
AU  - Jovanović, Predrag
AU  - Dronjak, Slađana
AU  - Marković, Bojan
AU  - Dondević, Tea
AU  - Ignjatović, Svetlana
AU  - Pešić, Vesna
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3012
AB  - Chronic stress conditions can lead to considerable and extensible changes in physiological and psychological performances, and in emergence of risk for various somatic diseases. On the other hand, the neuropeptide oxytocin is reported to increase the resistance of the organism to stress and modulate activity of autonomic nervous system. Chronic corticosterone administration is used as a rat model for a state observed in terms of chronic stress exposure, when negative feedback mechanism of hypothalamus-pituitary-adrenal axis activity is disrupted. In our study, we aimed to investigate whether chronic administration of oxytocin (10114400 pI/day for 14 days, s.c.) influenced adrenal gland morphology and activity in adult male Wistar rats during long-term corticosterone administration via drinking water (100 mg/L for 21 days). We examined the influence of treatments on the levels of adrenal gland hormones, corticosterone, adrenaline and noradrenaline, as well as their response to an acute stress challenge evoked by 15-min forced swimming. In addition, the expression of two main monoamine transporters, the noradrenaline transporter (NAT) and vesicular monoamine transporter 2 (VMAT2) in adrenal medulla was measured in the rats exposed to acute stress. Our results showed that oxytocin treatment prevented corticosterone-induced decrease in body weight gain, attenuated adrenal gland atrophy by increasing glandular weight, and the area of the zona fasciculate and reticularis. Chronic corticosterone intake blunted the response of all measured hormones to acute stress, whereas concomitant oxytocin treatment reversed adrenaline and noradrenaline response to acute stress. Furthermore, in adrenal medulla, oxytocin produced significant vasodilatation and stimulated expression of both catecholamine transporters detected both on mRNA and protein level. Our data suggest that oxytocin, by reducing atrophy of adrenal gland, and by increasing catecholamine storage capacity, may be beneficial in conditions accompanied with high glucocorticoid levels, such as chronic stress exposure.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Psychopharmacology
T1  - Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function
VL  - 80
SP  - 137
EP  - 146
DO  - 10.1016/j.psyneuen.2017.03.011
ER  - 

@article{
author = "Stanić, Dušanka and Plećaš-Solarović, Bosiljka and Mirković, Duško and Jovanović, Predrag and Dronjak, Slađana and Marković, Bojan and Dondević, Tea and Ignjatović, Svetlana and Pešić, Vesna",
year = "2017",
abstract = "Chronic stress conditions can lead to considerable and extensible changes in physiological and psychological performances, and in emergence of risk for various somatic diseases. On the other hand, the neuropeptide oxytocin is reported to increase the resistance of the organism to stress and modulate activity of autonomic nervous system. Chronic corticosterone administration is used as a rat model for a state observed in terms of chronic stress exposure, when negative feedback mechanism of hypothalamus-pituitary-adrenal axis activity is disrupted. In our study, we aimed to investigate whether chronic administration of oxytocin (10114400 pI/day for 14 days, s.c.) influenced adrenal gland morphology and activity in adult male Wistar rats during long-term corticosterone administration via drinking water (100 mg/L for 21 days). We examined the influence of treatments on the levels of adrenal gland hormones, corticosterone, adrenaline and noradrenaline, as well as their response to an acute stress challenge evoked by 15-min forced swimming. In addition, the expression of two main monoamine transporters, the noradrenaline transporter (NAT) and vesicular monoamine transporter 2 (VMAT2) in adrenal medulla was measured in the rats exposed to acute stress. Our results showed that oxytocin treatment prevented corticosterone-induced decrease in body weight gain, attenuated adrenal gland atrophy by increasing glandular weight, and the area of the zona fasciculate and reticularis. Chronic corticosterone intake blunted the response of all measured hormones to acute stress, whereas concomitant oxytocin treatment reversed adrenaline and noradrenaline response to acute stress. Furthermore, in adrenal medulla, oxytocin produced significant vasodilatation and stimulated expression of both catecholamine transporters detected both on mRNA and protein level. Our data suggest that oxytocin, by reducing atrophy of adrenal gland, and by increasing catecholamine storage capacity, may be beneficial in conditions accompanied with high glucocorticoid levels, such as chronic stress exposure.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Psychopharmacology",
title = "Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function",
volume = "80",
pages = "137-146",
doi = "10.1016/j.psyneuen.2017.03.011"
}

Stanić, D., Plećaš-Solarović, B., Mirković, D., Jovanović, P., Dronjak, S., Marković, B., Dondević, T., Ignjatović, S.,& Pešić, V.. (2017). Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function. in Psychopharmacology
Pergamon-Elsevier Science Ltd, Oxford., 80, 137-146.
https://doi.org/10.1016/j.psyneuen.2017.03.011

Stanić D, Plećaš-Solarović B, Mirković D, Jovanović P, Dronjak S, Marković B, Dondević T, Ignjatović S, Pešić V. Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function. in Psychopharmacology. 2017;80:137-146.
doi:10.1016/j.psyneuen.2017.03.011 .

Stanić, Dušanka, Plećaš-Solarović, Bosiljka, Mirković, Duško, Jovanović, Predrag, Dronjak, Slađana, Marković, Bojan, Dondević, Tea, Ignjatović, Svetlana, Pešić, Vesna, "Oxytocin in corticosterone-induced chronic stress model: Focus on adrenal gland function" in Psychopharmacology, 80 (2017):137-146,
https://doi.org/10.1016/j.psyneuen.2017.03.011 . .

TY  - JOUR
AU  - Petrović, Jelena
AU  - Stanić, Dušanka
AU  - Dmitrašinović, Gordana
AU  - Plećaš-Solarović, Bosiljka
AU  - Ignjatović, Svetlana
AU  - Batinić, Bojan
AU  - Popović, Dejana
AU  - Pešić, Vesna
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2557
AB  - Sedentary lifestyle is highly associated with increased risk of cardiovascular disease, obesity, and type 2 diabetes. It is known that regular physical activity has positive effects on health; however several studies have shown that acute and strenuous exercise can induce oxidative stress and lead to DNA damage. As magnesium is essential in maintaining DNA integrity, the aim of this study was to determine whether four-week-long magnesium supplementation in students with sedentary lifestyle and rugby players could prevent or diminish impairment of DNA. By using the comet assay, our study demonstrated that the number of peripheral blood lymphocytes (PBL) with basal endogenous DNA damage is significantly higher in rugby players compared to students with sedentary lifestyle. On the other hand, magnesium supplementation significantly decreased the number of cells with high DNA damage, in the presence of exogenous H2O2, in PBL from both students and rugby players, and markedly reduced the number of cells with medium DNA damage in rugby players compared to corresponding control nonsupplemented group. Accordingly, the results of our study suggest that four-week-long magnesium supplementation has marked effects in protecting the DNA from oxidative damage in both rugby players and in young men with sedentary lifestyle.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Magnesium Supplementation Diminishes Peripheral Blood Lymphocyte DNA Oxidative Damage in Athletes and Sedentary Young Man
DO  - 10.1155/2016/2019643
ER  - 

@article{
author = "Petrović, Jelena and Stanić, Dušanka and Dmitrašinović, Gordana and Plećaš-Solarović, Bosiljka and Ignjatović, Svetlana and Batinić, Bojan and Popović, Dejana and Pešić, Vesna",
year = "2016",
abstract = "Sedentary lifestyle is highly associated with increased risk of cardiovascular disease, obesity, and type 2 diabetes. It is known that regular physical activity has positive effects on health; however several studies have shown that acute and strenuous exercise can induce oxidative stress and lead to DNA damage. As magnesium is essential in maintaining DNA integrity, the aim of this study was to determine whether four-week-long magnesium supplementation in students with sedentary lifestyle and rugby players could prevent or diminish impairment of DNA. By using the comet assay, our study demonstrated that the number of peripheral blood lymphocytes (PBL) with basal endogenous DNA damage is significantly higher in rugby players compared to students with sedentary lifestyle. On the other hand, magnesium supplementation significantly decreased the number of cells with high DNA damage, in the presence of exogenous H2O2, in PBL from both students and rugby players, and markedly reduced the number of cells with medium DNA damage in rugby players compared to corresponding control nonsupplemented group. Accordingly, the results of our study suggest that four-week-long magnesium supplementation has marked effects in protecting the DNA from oxidative damage in both rugby players and in young men with sedentary lifestyle.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Magnesium Supplementation Diminishes Peripheral Blood Lymphocyte DNA Oxidative Damage in Athletes and Sedentary Young Man",
doi = "10.1155/2016/2019643"
}

Petrović, J., Stanić, D., Dmitrašinović, G., Plećaš-Solarović, B., Ignjatović, S., Batinić, B., Popović, D.,& Pešić, V.. (2016). Magnesium Supplementation Diminishes Peripheral Blood Lymphocyte DNA Oxidative Damage in Athletes and Sedentary Young Man. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London..
https://doi.org/10.1155/2016/2019643

Petrović J, Stanić D, Dmitrašinović G, Plećaš-Solarović B, Ignjatović S, Batinić B, Popović D, Pešić V. Magnesium Supplementation Diminishes Peripheral Blood Lymphocyte DNA Oxidative Damage in Athletes and Sedentary Young Man. in Oxidative Medicine and Cellular Longevity. 2016;.
doi:10.1155/2016/2019643 .

Petrović, Jelena, Stanić, Dušanka, Dmitrašinović, Gordana, Plećaš-Solarović, Bosiljka, Ignjatović, Svetlana, Batinić, Bojan, Popović, Dejana, Pešić, Vesna, "Magnesium Supplementation Diminishes Peripheral Blood Lymphocyte DNA Oxidative Damage in Athletes and Sedentary Young Man" in Oxidative Medicine and Cellular Longevity (2016),
https://doi.org/10.1155/2016/2019643 . .

TY  - JOUR
AU  - Stanić, Dušanka
AU  - Plećaš-Solarović, Bosiljka
AU  - Petrović, Jelena
AU  - Bogavac-Stanojević, Nataša
AU  - Sopić, Miron
AU  - Kotur-Stevuljević, Jelena
AU  - Ignjatović, Svetlana
AU  - Pešić, Vesna
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2704
AB  - Contemporary lifestyle is commonly associated with chronic stress, an environmental factor contributing to development of various psychological and somatic disorders. Increased levels of glucocorticoids, observed in the chronic stress, induce the production of reactive oxygen species leading to genotoxicity. The aim of this study was to investigate whether chronic administration of oxytocin (OXY) 10 IU/400 mu L/day, s.c., for 14 days, a hormone presumed to exert antioxidant effect, may prevent DNA damage in the comet assay of peripheral blood lymphocytes of Wistar rats treated chronically with corticosterone (CORT) 100 mg/L ad libitum, per os, for 21 days, as well as, to influence some plasma oxidative stress parameters, i.e. levels of total lipid hydroperoxide (LOOH), and malondialdehyde (MDA), and the activity of antioxidative enzyme superoxide dismutase (SOD). Even though there was no reduction in overall number of damaged cells after oxytocin treatment only, the marked increase in total comet score (TCS) after incubation with H2O2 in CORT group compared to controls, was absent in the CORT + OXY experimental group. Furthermore, significant decrease of highly damaged cells compared to corticosterone group was noted. Chronic oxytocin administration thus protected lymphocytes from high intensity damage that leads to cellular death. In addition, treatment with OXY along with CORT, significantly decreased concentration of LOOH in plasma, and increased SOD compared to CORT treatment only. This finding corresponds well with current reports on beneficial effects of OXY in conditions of HPA axis hyperactivity, and supports the hypothesis of OXY-mediated antioxidant action.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment
VL  - 256
SP  - 134
EP  - 141
DO  - 10.1016/j.cbi.2016.07.006
ER  - 

@article{
author = "Stanić, Dušanka and Plećaš-Solarović, Bosiljka and Petrović, Jelena and Bogavac-Stanojević, Nataša and Sopić, Miron and Kotur-Stevuljević, Jelena and Ignjatović, Svetlana and Pešić, Vesna",
year = "2016",
abstract = "Contemporary lifestyle is commonly associated with chronic stress, an environmental factor contributing to development of various psychological and somatic disorders. Increased levels of glucocorticoids, observed in the chronic stress, induce the production of reactive oxygen species leading to genotoxicity. The aim of this study was to investigate whether chronic administration of oxytocin (OXY) 10 IU/400 mu L/day, s.c., for 14 days, a hormone presumed to exert antioxidant effect, may prevent DNA damage in the comet assay of peripheral blood lymphocytes of Wistar rats treated chronically with corticosterone (CORT) 100 mg/L ad libitum, per os, for 21 days, as well as, to influence some plasma oxidative stress parameters, i.e. levels of total lipid hydroperoxide (LOOH), and malondialdehyde (MDA), and the activity of antioxidative enzyme superoxide dismutase (SOD). Even though there was no reduction in overall number of damaged cells after oxytocin treatment only, the marked increase in total comet score (TCS) after incubation with H2O2 in CORT group compared to controls, was absent in the CORT + OXY experimental group. Furthermore, significant decrease of highly damaged cells compared to corticosterone group was noted. Chronic oxytocin administration thus protected lymphocytes from high intensity damage that leads to cellular death. In addition, treatment with OXY along with CORT, significantly decreased concentration of LOOH in plasma, and increased SOD compared to CORT treatment only. This finding corresponds well with current reports on beneficial effects of OXY in conditions of HPA axis hyperactivity, and supports the hypothesis of OXY-mediated antioxidant action.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment",
volume = "256",
pages = "134-141",
doi = "10.1016/j.cbi.2016.07.006"
}

Stanić, D., Plećaš-Solarović, B., Petrović, J., Bogavac-Stanojević, N., Sopić, M., Kotur-Stevuljević, J., Ignjatović, S.,& Pešić, V.. (2016). Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 256, 134-141.
https://doi.org/10.1016/j.cbi.2016.07.006

Stanić D, Plećaš-Solarović B, Petrović J, Bogavac-Stanojević N, Sopić M, Kotur-Stevuljević J, Ignjatović S, Pešić V. Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment. in Chemico-Biological Interactions. 2016;256:134-141.
doi:10.1016/j.cbi.2016.07.006 .

Stanić, Dušanka, Plećaš-Solarović, Bosiljka, Petrović, Jelena, Bogavac-Stanojević, Nataša, Sopić, Miron, Kotur-Stevuljević, Jelena, Ignjatović, Svetlana, Pešić, Vesna, "Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment" in Chemico-Biological Interactions, 256 (2016):134-141,
https://doi.org/10.1016/j.cbi.2016.07.006 . .

TY  - JOUR
AU  - Dmitrasinović, Gordana
AU  - Pešić, Vesna
AU  - Stanić, Dušanka
AU  - Plećaš-Solarović, Bosiljka
AU  - Dajak, Marijana
AU  - Ignjatović, Svetlana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2748
AB  - Background: Physical exercise activates the hypothalamopituitary-adrenal (HPA) axis and induces the body's inflammatory response. Due to contemporary dietary habits and increased energy expenditure, athletes are susceptible to depletion of magnesium ions. The aim of our study was to investigate, through assessment of plasma ACTH, serum IL-6, and salivary/serum cortisol levels, if chronic magnesium supplementation might reduce damaging stress effects in amateur rugby players. Methods: Rugby players (N=23) were randomly assigned to intervention and control group. Basal samples were collected before intervention group started a 4-week-long supplementation with magnesium (500 mg Mg/d). Blood and saliva sampling were done a day before the match (Day-1), on the morning of competition (Game), and during a six-day-long recovery period (Day1, Day3 and Day6). ACTH, serum/salivary cortisol, IL-6 and total/differential leukocytes counts were determined at each time point. Results: There was a statistically significant increase in ACTH concentration in intervention group compared to control group, while reductions in cortisol concentrations between the two groups were the greatest at Day-1 (p lt 0.01) and at the day of competition (Game) (p lt 0.01). Our results revealed that magnesium completely abolished the increase in IL-6 level noted in control group on Day1 and Day3 vs. Day-1 (p lt 0.01) and also diminished the rise in neutrophil/lymphocyte ratio in intervention group vs. control group (p lt 0.01). Conclusions: These results suggest the possibly important influence magnesium supplementation might have on the change of parameters of HPA axis activity and reduction of immune response activation following strenuous physical exercise such as a rugby game.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - ACTH, Cortisol and IL-6 Levels in Athletes Following Magnesium Supplementation
VL  - 35
IS  - 4
SP  - 375
EP  - 384
DO  - 10.1515/jomb-2016-0021
ER  - 

@article{
author = "Dmitrasinović, Gordana and Pešić, Vesna and Stanić, Dušanka and Plećaš-Solarović, Bosiljka and Dajak, Marijana and Ignjatović, Svetlana",
year = "2016",
abstract = "Background: Physical exercise activates the hypothalamopituitary-adrenal (HPA) axis and induces the body's inflammatory response. Due to contemporary dietary habits and increased energy expenditure, athletes are susceptible to depletion of magnesium ions. The aim of our study was to investigate, through assessment of plasma ACTH, serum IL-6, and salivary/serum cortisol levels, if chronic magnesium supplementation might reduce damaging stress effects in amateur rugby players. Methods: Rugby players (N=23) were randomly assigned to intervention and control group. Basal samples were collected before intervention group started a 4-week-long supplementation with magnesium (500 mg Mg/d). Blood and saliva sampling were done a day before the match (Day-1), on the morning of competition (Game), and during a six-day-long recovery period (Day1, Day3 and Day6). ACTH, serum/salivary cortisol, IL-6 and total/differential leukocytes counts were determined at each time point. Results: There was a statistically significant increase in ACTH concentration in intervention group compared to control group, while reductions in cortisol concentrations between the two groups were the greatest at Day-1 (p lt 0.01) and at the day of competition (Game) (p lt 0.01). Our results revealed that magnesium completely abolished the increase in IL-6 level noted in control group on Day1 and Day3 vs. Day-1 (p lt 0.01) and also diminished the rise in neutrophil/lymphocyte ratio in intervention group vs. control group (p lt 0.01). Conclusions: These results suggest the possibly important influence magnesium supplementation might have on the change of parameters of HPA axis activity and reduction of immune response activation following strenuous physical exercise such as a rugby game.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "ACTH, Cortisol and IL-6 Levels in Athletes Following Magnesium Supplementation",
volume = "35",
number = "4",
pages = "375-384",
doi = "10.1515/jomb-2016-0021"
}

Dmitrasinović, G., Pešić, V., Stanić, D., Plećaš-Solarović, B., Dajak, M.,& Ignjatović, S.. (2016). ACTH, Cortisol and IL-6 Levels in Athletes Following Magnesium Supplementation. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 35(4), 375-384.
https://doi.org/10.1515/jomb-2016-0021

Dmitrasinović G, Pešić V, Stanić D, Plećaš-Solarović B, Dajak M, Ignjatović S. ACTH, Cortisol and IL-6 Levels in Athletes Following Magnesium Supplementation. in Journal of Medical Biochemistry. 2016;35(4):375-384.
doi:10.1515/jomb-2016-0021 .

Dmitrasinović, Gordana, Pešić, Vesna, Stanić, Dušanka, Plećaš-Solarović, Bosiljka, Dajak, Marijana, Ignjatović, Svetlana, "ACTH, Cortisol and IL-6 Levels in Athletes Following Magnesium Supplementation" in Journal of Medical Biochemistry, 35, no. 4 (2016):375-384,
https://doi.org/10.1515/jomb-2016-0021 . .

TY  - JOUR
AU  - Popović, Dejana
AU  - Damjanović, Svetozar S.
AU  - Plećaš-Solarović, Bosiljka
AU  - Pešić, Vesna
AU  - Stojiljković, Stanimir
AU  - Banović, Marko
AU  - Ristić, Arsen
AU  - Mantegazza, Valentina
AU  - Agostoni, Piergiuseppe
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2619
AB  - The usage of alcohol is widespread, but the effects of acute alcohol ingestion on exercise performance and the stress hormone axis are not fully elucidated.We studied 10 healthy white men, nonhabitual drinkers, by Doppler echocardiography at rest, spirometry, and maximal cardiopulmonary exercise test (CPET) in two visits (2-4 days in between), one after administration of 1.5g/kg ethanol (whisky) diluted at 15% in water, and the other after administration of an equivalent volume of water. Plasma levels of NT-pro-BNP, cortisol, and adrenocorticotropic hormone (ACTH) were also measured 10min before the test, at maximal effort and at the third minute of recovery. Ethanol concentration was measured from resting blood samples by gas chromatography and it increased from 0.00 +/- 0.00 to 1.25 +/- 0.54 parts per thousand (P lt 0.001). Basal echocardiographic and spirometric parameters were normal and remained so after acute alcohol intake, whereas ACTH, cortisol, and NT-pro-BNP nonsignificantly increased in all phases of the test. CPET data suggested a trend toward a slight reduction of exercise performance (peak VO2=3008 +/- 638 vs. 2900 +/- 543ml/min, ns; peak workload=269 +/- 53 vs. 249 +/- 40W, ns; test duration 13.7 +/- 2.2 vs. 13.3 +/- 1.7min, ns; VE/VCO2 22.1 +/- 1.4 vs. 23.3 +/- 2.9, ns). Ventilatory equivalent for carbon dioxide at rest was higher after alcohol intake (28 +/- 2.5 vs. 30.4 +/- 3.2, P=0.039) and maximal respiratory exchange ratio was lower after alcohol intake (1.17 +/- 0.02 vs. 1.14 +/- 0.04, P=0.04). In conclusion, we showed that acute alcohol intake in healthy white men is associated with a nonsignificant exercise performance reduction and stress hormone stimulation, with an unchanged exercise metabolism.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Cardiovascular Medicine
T1  - Exercise capacity is not impaired after acute alcohol ingestion: a pilot study
VL  - 17
IS  - 12
SP  - 896
EP  - 901
DO  - 10.2459/JCM.0000000000000151
ER  - 

@article{
author = "Popović, Dejana and Damjanović, Svetozar S. and Plećaš-Solarović, Bosiljka and Pešić, Vesna and Stojiljković, Stanimir and Banović, Marko and Ristić, Arsen and Mantegazza, Valentina and Agostoni, Piergiuseppe",
year = "2016",
abstract = "The usage of alcohol is widespread, but the effects of acute alcohol ingestion on exercise performance and the stress hormone axis are not fully elucidated.We studied 10 healthy white men, nonhabitual drinkers, by Doppler echocardiography at rest, spirometry, and maximal cardiopulmonary exercise test (CPET) in two visits (2-4 days in between), one after administration of 1.5g/kg ethanol (whisky) diluted at 15% in water, and the other after administration of an equivalent volume of water. Plasma levels of NT-pro-BNP, cortisol, and adrenocorticotropic hormone (ACTH) were also measured 10min before the test, at maximal effort and at the third minute of recovery. Ethanol concentration was measured from resting blood samples by gas chromatography and it increased from 0.00 +/- 0.00 to 1.25 +/- 0.54 parts per thousand (P lt 0.001). Basal echocardiographic and spirometric parameters were normal and remained so after acute alcohol intake, whereas ACTH, cortisol, and NT-pro-BNP nonsignificantly increased in all phases of the test. CPET data suggested a trend toward a slight reduction of exercise performance (peak VO2=3008 +/- 638 vs. 2900 +/- 543ml/min, ns; peak workload=269 +/- 53 vs. 249 +/- 40W, ns; test duration 13.7 +/- 2.2 vs. 13.3 +/- 1.7min, ns; VE/VCO2 22.1 +/- 1.4 vs. 23.3 +/- 2.9, ns). Ventilatory equivalent for carbon dioxide at rest was higher after alcohol intake (28 +/- 2.5 vs. 30.4 +/- 3.2, P=0.039) and maximal respiratory exchange ratio was lower after alcohol intake (1.17 +/- 0.02 vs. 1.14 +/- 0.04, P=0.04). In conclusion, we showed that acute alcohol intake in healthy white men is associated with a nonsignificant exercise performance reduction and stress hormone stimulation, with an unchanged exercise metabolism.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Cardiovascular Medicine",
title = "Exercise capacity is not impaired after acute alcohol ingestion: a pilot study",
volume = "17",
number = "12",
pages = "896-901",
doi = "10.2459/JCM.0000000000000151"
}

Popović, D., Damjanović, S. S., Plećaš-Solarović, B., Pešić, V., Stojiljković, S., Banović, M., Ristić, A., Mantegazza, V.,& Agostoni, P.. (2016). Exercise capacity is not impaired after acute alcohol ingestion: a pilot study. in Journal of Cardiovascular Medicine
Lippincott Williams & Wilkins, Philadelphia., 17(12), 896-901.
https://doi.org/10.2459/JCM.0000000000000151

Popović D, Damjanović SS, Plećaš-Solarović B, Pešić V, Stojiljković S, Banović M, Ristić A, Mantegazza V, Agostoni P. Exercise capacity is not impaired after acute alcohol ingestion: a pilot study. in Journal of Cardiovascular Medicine. 2016;17(12):896-901.
doi:10.2459/JCM.0000000000000151 .

Popović, Dejana, Damjanović, Svetozar S., Plećaš-Solarović, Bosiljka, Pešić, Vesna, Stojiljković, Stanimir, Banović, Marko, Ristić, Arsen, Mantegazza, Valentina, Agostoni, Piergiuseppe, "Exercise capacity is not impaired after acute alcohol ingestion: a pilot study" in Journal of Cardiovascular Medicine, 17, no. 12 (2016):896-901,
https://doi.org/10.2459/JCM.0000000000000151 . .

TY  - JOUR
AU  - Popović, Dejana
AU  - Plećaš-Solarović, Bosiljka
AU  - Pešić, Vesna
AU  - Petrović, Milan
AU  - Vujisić-Tesić, Bosiljka
AU  - Popović, Bojana
AU  - Ignjatović, Svetlana
AU  - Ristić, Arsen
AU  - Damjanović, Svetozar S.
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2105
AB  - This aim of this study was to evaluate the predictive value of adrenocorticotropic hormone (ACTH), cortisol and ACTH receptor polymorphism (ACTHRP) for left ventricular (LV) remodeling. Thirty-six elite male athletes, as chronic stress adaptation models, and twenty sedentary age and sex-mached subjects emabarked on standard and tissue Doppler echocardiography to assess cardiac parameters at rest. They performed maximal cardiopulmonary test, which was used as an acute stress model. ACTH and cortisol were measured at rest (10 min before test), at beginning, at maximal effort, at 3rd min of recovery, using radioimmunometric and radioimmunoassey techniques, respectively. Promoter region of ACTHR gene (18p11.2) was analysed from blood samples using reverse polymerization reaction with the analysis of restriction fragment length polimorphisam by SacI restriction enzyme. Normal genotype was CTC/CTC, heterozygot for ACTHRP CTC/CCC and homozygot CCC/CCC. In all participants, ACTH and cortisol increased during acute stress, whereas in recovery ACTH increased and cortisol remained unchanged. 49/56 examiners manifested CTC/CTC, 7/56 CTC/CCC and 0/56 CCC/CCC. There was no difference in ACTHRP frequency between groups (chi(2)((1))= 0.178, p = 0.67). LV mass (LVM) and LV end-diastolic volume (LVVd) were higher in athletes than in controls (p  lt  0.01) and lower in CTC/CTC than in CTC/CCC genotype (219.43 +/- 46.59(SD)g vs. 276.34 +/- 48.86(SD)g, p = 0.004; 141.24 +/- 24.46(SD)ml vs. 175.29 +/- 37.07(SD)ml, p = 0.002; respectively). In all participants, predictors of LVM and LVVd were ACTH at rest (B = -1.00, -0.44; beta= -0.30, -0.31; p = 0.026,0.012, respectively) and ACTHRP (B = 56.63,34; beta= 0.37,0.40; p = 0.003,0.001, respectively). These results demonstrate that ACTH and ACTHRP strongly predict cardiac morphology suggesting possible regulatory role of stress system activity and sensitivity in cardiac remodeling.
PB  - Elsevier Science Inc, New York
T2  - Peptides
T1  - How does stress possibly affect cardiac remodeling?
VL  - 57
SP  - 20
EP  - 30
DO  - 10.1016/j.peptides.2014.04.006
ER  - 

@article{
author = "Popović, Dejana and Plećaš-Solarović, Bosiljka and Pešić, Vesna and Petrović, Milan and Vujisić-Tesić, Bosiljka and Popović, Bojana and Ignjatović, Svetlana and Ristić, Arsen and Damjanović, Svetozar S.",
year = "2014",
abstract = "This aim of this study was to evaluate the predictive value of adrenocorticotropic hormone (ACTH), cortisol and ACTH receptor polymorphism (ACTHRP) for left ventricular (LV) remodeling. Thirty-six elite male athletes, as chronic stress adaptation models, and twenty sedentary age and sex-mached subjects emabarked on standard and tissue Doppler echocardiography to assess cardiac parameters at rest. They performed maximal cardiopulmonary test, which was used as an acute stress model. ACTH and cortisol were measured at rest (10 min before test), at beginning, at maximal effort, at 3rd min of recovery, using radioimmunometric and radioimmunoassey techniques, respectively. Promoter region of ACTHR gene (18p11.2) was analysed from blood samples using reverse polymerization reaction with the analysis of restriction fragment length polimorphisam by SacI restriction enzyme. Normal genotype was CTC/CTC, heterozygot for ACTHRP CTC/CCC and homozygot CCC/CCC. In all participants, ACTH and cortisol increased during acute stress, whereas in recovery ACTH increased and cortisol remained unchanged. 49/56 examiners manifested CTC/CTC, 7/56 CTC/CCC and 0/56 CCC/CCC. There was no difference in ACTHRP frequency between groups (chi(2)((1))= 0.178, p = 0.67). LV mass (LVM) and LV end-diastolic volume (LVVd) were higher in athletes than in controls (p  lt  0.01) and lower in CTC/CTC than in CTC/CCC genotype (219.43 +/- 46.59(SD)g vs. 276.34 +/- 48.86(SD)g, p = 0.004; 141.24 +/- 24.46(SD)ml vs. 175.29 +/- 37.07(SD)ml, p = 0.002; respectively). In all participants, predictors of LVM and LVVd were ACTH at rest (B = -1.00, -0.44; beta= -0.30, -0.31; p = 0.026,0.012, respectively) and ACTHRP (B = 56.63,34; beta= 0.37,0.40; p = 0.003,0.001, respectively). These results demonstrate that ACTH and ACTHRP strongly predict cardiac morphology suggesting possible regulatory role of stress system activity and sensitivity in cardiac remodeling.",
publisher = "Elsevier Science Inc, New York",
journal = "Peptides",
title = "How does stress possibly affect cardiac remodeling?",
volume = "57",
pages = "20-30",
doi = "10.1016/j.peptides.2014.04.006"
}

Popović, D., Plećaš-Solarović, B., Pešić, V., Petrović, M., Vujisić-Tesić, B., Popović, B., Ignjatović, S., Ristić, A.,& Damjanović, S. S.. (2014). How does stress possibly affect cardiac remodeling?. in Peptides
Elsevier Science Inc, New York., 57, 20-30.
https://doi.org/10.1016/j.peptides.2014.04.006

Popović D, Plećaš-Solarović B, Pešić V, Petrović M, Vujisić-Tesić B, Popović B, Ignjatović S, Ristić A, Damjanović SS. How does stress possibly affect cardiac remodeling?. in Peptides. 2014;57:20-30.
doi:10.1016/j.peptides.2014.04.006 .

Popović, Dejana, Plećaš-Solarović, Bosiljka, Pešić, Vesna, Petrović, Milan, Vujisić-Tesić, Bosiljka, Popović, Bojana, Ignjatović, Svetlana, Ristić, Arsen, Damjanović, Svetozar S., "How does stress possibly affect cardiac remodeling?" in Peptides, 57 (2014):20-30,
https://doi.org/10.1016/j.peptides.2014.04.006 . .

TY  - JOUR
AU  - Plećaš-Solarović, Bosiljka
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
AU  - Potparević, Biljana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2088
AB  - Alzheimer's disease (AD) is the most frequent progressive neurodegenerative disorder in elderly associated with irreversible cognitive impairment and dementia. The vast majority of AD patients are sporadic (SAD) in which the disease develops after age of 65. Despite of century of research, we lack understanding of the SAD etiology and pathogenesis. Several hypotheses try to explain the main causes of brain degeneration in SAD, one of them assuming that genomic instability and the reentry of certain neurons into the incomplete cell cycle may be the pathogenic basis of the disease. Although the brain is the most affected organ in AD, numerous studies showed structural and functional alterations in peripheral tissues, suggesting that AD is a generalized systemic disorder. Diverse changes in peripheral cells from AD patients are described in literature including cell cycle aberration and chromosome instability, alterations in cell viability, proliferation and apoptosis, oxidative metabolism, amyloid precursor protein and amyloid beta protein metabolism, and other cellular processes. The aim of this paper was to summarize and review the results of our investigations and the growing literature data concerning the multiple chromosomal alterations in peripheral cells of AD patients and to consider their possible role in the disease pathogenesis as well as the importance of such investigations.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika, Belgrade
T1  - Cytogenetic alterations in peripheral cells of alzheimer's disease patients
VL  - 46
IS  - 1
SP  - 315
EP  - 330
DO  - 10.2298/GENSR1401315P
ER  - 

@article{
author = "Plećaš-Solarović, Bosiljka and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada and Potparević, Biljana",
year = "2014",
abstract = "Alzheimer's disease (AD) is the most frequent progressive neurodegenerative disorder in elderly associated with irreversible cognitive impairment and dementia. The vast majority of AD patients are sporadic (SAD) in which the disease develops after age of 65. Despite of century of research, we lack understanding of the SAD etiology and pathogenesis. Several hypotheses try to explain the main causes of brain degeneration in SAD, one of them assuming that genomic instability and the reentry of certain neurons into the incomplete cell cycle may be the pathogenic basis of the disease. Although the brain is the most affected organ in AD, numerous studies showed structural and functional alterations in peripheral tissues, suggesting that AD is a generalized systemic disorder. Diverse changes in peripheral cells from AD patients are described in literature including cell cycle aberration and chromosome instability, alterations in cell viability, proliferation and apoptosis, oxidative metabolism, amyloid precursor protein and amyloid beta protein metabolism, and other cellular processes. The aim of this paper was to summarize and review the results of our investigations and the growing literature data concerning the multiple chromosomal alterations in peripheral cells of AD patients and to consider their possible role in the disease pathogenesis as well as the importance of such investigations.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika, Belgrade",
title = "Cytogenetic alterations in peripheral cells of alzheimer's disease patients",
volume = "46",
number = "1",
pages = "315-330",
doi = "10.2298/GENSR1401315P"
}

Plećaš-Solarović, B., Đelić, N., Bajić, V., Živković, L.,& Potparević, B.. (2014). Cytogenetic alterations in peripheral cells of alzheimer's disease patients. in Genetika, Belgrade
Društvo genetičara Srbije, Beograd., 46(1), 315-330.
https://doi.org/10.2298/GENSR1401315P

Plećaš-Solarović B, Đelić N, Bajić V, Živković L, Potparević B. Cytogenetic alterations in peripheral cells of alzheimer's disease patients. in Genetika, Belgrade. 2014;46(1):315-330.
doi:10.2298/GENSR1401315P .

Plećaš-Solarović, Bosiljka, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, Potparević, Biljana, "Cytogenetic alterations in peripheral cells of alzheimer's disease patients" in Genetika, Belgrade, 46, no. 1 (2014):315-330,
https://doi.org/10.2298/GENSR1401315P . .

TY  - JOUR
AU  - Popović, Dejana
AU  - Popović, Bojana
AU  - Plećaš-Solarović, Bosiljka
AU  - Pešić, Vesna
AU  - Marković, Vidan
AU  - Stojiljković, Stanimir
AU  - Vukcević, Vladan
AU  - Petrović, Ivana
AU  - Banović, Marko
AU  - Petrović, Milan
AU  - Vujisić-Tesić, Bosiljka
AU  - Ostojić, Miodrag
AU  - Ristić, Arsen
AU  - Damjanović, Svetozar S.
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1857
AB  - Brain natriuretic peptide (NT-pro-BNP) was implicated in the regulation of hypothalamic-pituitary-adrenocortical (HPA) responses to psychological stressors. However, HPA axis activation in different physical stress models and its interface with NT-pro-BNP in the prediction of cardiopulmonary performance is unclear. Cardiopulmonary test on a treadmill was used to assess cardiopulmonary parameters in 16 elite male wrestlers (W), 21 water polo player (WP) and 20 sedentary age-matched subjects
PB  - Elsevier Science Inc, New York
T2  - Peptides
T1  - The interface of hypothalamic-pituitary-adrenocortical axis and circulating brain natriuretic peptide in prediction of cardiopulmonary performance during physical stress
VL  - 47
SP  - 85
EP  - 93
DO  - 10.1016/j.peptides.2013.07.009
ER  - 

@article{
author = "Popović, Dejana and Popović, Bojana and Plećaš-Solarović, Bosiljka and Pešić, Vesna and Marković, Vidan and Stojiljković, Stanimir and Vukcević, Vladan and Petrović, Ivana and Banović, Marko and Petrović, Milan and Vujisić-Tesić, Bosiljka and Ostojić, Miodrag and Ristić, Arsen and Damjanović, Svetozar S.",
year = "2013",
abstract = "Brain natriuretic peptide (NT-pro-BNP) was implicated in the regulation of hypothalamic-pituitary-adrenocortical (HPA) responses to psychological stressors. However, HPA axis activation in different physical stress models and its interface with NT-pro-BNP in the prediction of cardiopulmonary performance is unclear. Cardiopulmonary test on a treadmill was used to assess cardiopulmonary parameters in 16 elite male wrestlers (W), 21 water polo player (WP) and 20 sedentary age-matched subjects",
publisher = "Elsevier Science Inc, New York",
journal = "Peptides",
title = "The interface of hypothalamic-pituitary-adrenocortical axis and circulating brain natriuretic peptide in prediction of cardiopulmonary performance during physical stress",
volume = "47",
pages = "85-93",
doi = "10.1016/j.peptides.2013.07.009"
}

Popović, D., Popović, B., Plećaš-Solarović, B., Pešić, V., Marković, V., Stojiljković, S., Vukcević, V., Petrović, I., Banović, M., Petrović, M., Vujisić-Tesić, B., Ostojić, M., Ristić, A.,& Damjanović, S. S.. (2013). The interface of hypothalamic-pituitary-adrenocortical axis and circulating brain natriuretic peptide in prediction of cardiopulmonary performance during physical stress. in Peptides
Elsevier Science Inc, New York., 47, 85-93.
https://doi.org/10.1016/j.peptides.2013.07.009

Popović D, Popović B, Plećaš-Solarović B, Pešić V, Marković V, Stojiljković S, Vukcević V, Petrović I, Banović M, Petrović M, Vujisić-Tesić B, Ostojić M, Ristić A, Damjanović SS. The interface of hypothalamic-pituitary-adrenocortical axis and circulating brain natriuretic peptide in prediction of cardiopulmonary performance during physical stress. in Peptides. 2013;47:85-93.
doi:10.1016/j.peptides.2013.07.009 .

Popović, Dejana, Popović, Bojana, Plećaš-Solarović, Bosiljka, Pešić, Vesna, Marković, Vidan, Stojiljković, Stanimir, Vukcević, Vladan, Petrović, Ivana, Banović, Marko, Petrović, Milan, Vujisić-Tesić, Bosiljka, Ostojić, Miodrag, Ristić, Arsen, Damjanović, Svetozar S., "The interface of hypothalamic-pituitary-adrenocortical axis and circulating brain natriuretic peptide in prediction of cardiopulmonary performance during physical stress" in Peptides, 47 (2013):85-93,
https://doi.org/10.1016/j.peptides.2013.07.009 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Potparević, Biljana
AU  - Siedlak, Sandra L.
AU  - Perry, George
AU  - Plećaš-Solarović, Bosiljka
AU  - Milicević, Zorana
AU  - Bajić, Vladan
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1861
AB  - While Alzheimer disease (AD) is considered a neurodegenerative disorder, the importance of chromosome instability in non-neuronal cells is equally important, not only for shedding light on the etiology of the disease, but also for possible diagnostic purposes and monitoring the progress of the disease. Here, we evaluated the frequency of DNA damage and expression of premature centromere division (PCD) in peripheral blood lymphocytes of sporadic AD patients, age-matched and young controls. The results show that in male patients with AD, the frequencies of PCD and DNA damage were significantly greater (88%, p lt 0.01 and 38%, p lt 0.05, respectively) than in age-matched control group. AD females had significantly increased frequency of PCD (134%, p lt 0.01) as well as a higher frequency of DNA damage (37%, p lt 0.05). Ageing per se, both in males and females, shows significant increase of percentages of PCD (2.3 times, p lt 0.01 and 2.8 times, p lt 0.01, respectively) and DNA damage (63%, p lt 0.01 and 50%, p lt 0.01, respectively) comparing with young controls. In addition, a strong (R-2 = 0.873, n = 6) and significant (p lt 0.01) correlation between the frequencies of PCD and DNA damage was found in all examined groups. We may conclude that the increases in both parameters evaluated in this study are not only associated with normal ageing processes, but are markedly and significantly intensified in AD pathogenesis. Thus, our data support the view that AD is a generalized systemic disease, at least as for the increased DNA damage and PCD incidence in peripheral blood cells. copyright
PB  - Karger, Basel
T2  - Neurodegenerative Diseases
T1  - DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division
VL  - 12
IS  - 3
SP  - 156
EP  - 163
DO  - 10.1159/000346114
ER  - 

@article{
author = "Živković, Lada and Potparević, Biljana and Siedlak, Sandra L. and Perry, George and Plećaš-Solarović, Bosiljka and Milicević, Zorana and Bajić, Vladan",
year = "2013",
abstract = "While Alzheimer disease (AD) is considered a neurodegenerative disorder, the importance of chromosome instability in non-neuronal cells is equally important, not only for shedding light on the etiology of the disease, but also for possible diagnostic purposes and monitoring the progress of the disease. Here, we evaluated the frequency of DNA damage and expression of premature centromere division (PCD) in peripheral blood lymphocytes of sporadic AD patients, age-matched and young controls. The results show that in male patients with AD, the frequencies of PCD and DNA damage were significantly greater (88%, p lt 0.01 and 38%, p lt 0.05, respectively) than in age-matched control group. AD females had significantly increased frequency of PCD (134%, p lt 0.01) as well as a higher frequency of DNA damage (37%, p lt 0.05). Ageing per se, both in males and females, shows significant increase of percentages of PCD (2.3 times, p lt 0.01 and 2.8 times, p lt 0.01, respectively) and DNA damage (63%, p lt 0.01 and 50%, p lt 0.01, respectively) comparing with young controls. In addition, a strong (R-2 = 0.873, n = 6) and significant (p lt 0.01) correlation between the frequencies of PCD and DNA damage was found in all examined groups. We may conclude that the increases in both parameters evaluated in this study are not only associated with normal ageing processes, but are markedly and significantly intensified in AD pathogenesis. Thus, our data support the view that AD is a generalized systemic disease, at least as for the increased DNA damage and PCD incidence in peripheral blood cells. copyright",
publisher = "Karger, Basel",
journal = "Neurodegenerative Diseases",
title = "DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division",
volume = "12",
number = "3",
pages = "156-163",
doi = "10.1159/000346114"
}

Živković, L., Potparević, B., Siedlak, S. L., Perry, G., Plećaš-Solarović, B., Milicević, Z.,& Bajić, V.. (2013). DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division. in Neurodegenerative Diseases
Karger, Basel., 12(3), 156-163.
https://doi.org/10.1159/000346114

Živković L, Potparević B, Siedlak SL, Perry G, Plećaš-Solarović B, Milicević Z, Bajić V. DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division. in Neurodegenerative Diseases. 2013;12(3):156-163.
doi:10.1159/000346114 .

Živković, Lada, Potparević, Biljana, Siedlak, Sandra L., Perry, George, Plećaš-Solarović, Bosiljka, Milicević, Zorana, Bajić, Vladan, "DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division" in Neurodegenerative Diseases, 12, no. 3 (2013):156-163,
https://doi.org/10.1159/000346114 . .

TY  - JOUR
AU  - Radaković, Milena
AU  - Đelić, Ninoslav
AU  - Stanimirović, Zoran
AU  - Plećaš-Solarović, Bosiljka
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Bajić, Vladan
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1607
AB  - Ephedrine, a natural alkaloid from plants of the genus Ephedra, has a chemical structure similar to catecholamines. It is well established that catecholamines (adraneline, noradrenaline and dopamine) cause genotoxic and mutagenic effects. Therefore, the objectives of this investigation were to examine weather ephedrine can exhibit genotoxic effects on isolated human lymphocytes in the Comet assay. Dose-response of human lymphocytes was determined at the concentration range of ephedrine from 0.0005 μM to 500 μM. Three concentrations of ephedrine (1, 50 and 300 μM) which had acceptable cell viability (over 90%) were used for further experiments with inhibitors of DNA reparation (cytosine arabinoside and hydroxyurea). The obtained results showed that ephedrine did not induce DNA damage in isolated human lymphocytes. However, co-treatment of the negative control with DNA repair inhibitors caused a slight but significant increase of DNA damage, due to an endogenous DNA damage. Interestingly, cells treated with ephedrine and DNA repair inhibitors did not express increased DNA damage. On the basis of the obtained results it can be concluded that ephedrine did not exhibit genotoxic effects on isolated human lymphocytes. This result is in accordance with previous investigations showing negative genotoxicological results for ephedrine using bacterial gene mutation test-systems and in vitro cytogenetic analysis.
AB  - Efedrin, prirodni alkaloid iz biljaka roda Ephedra, ima sličnu hemijsku strukturu sa kateholaminima. Dobro je poznato da kateholamini (adrenalin, noradrenalin i dopamin) mogu da prouzrokuju genotoksične i mutagene efekte. Stoga su ciljevi ovog istraživanja bili da se ispita da li efedrin može da ispolji genotoksične efekte na izolovanim limfocitima čoveka u Komet testu. Odnos doza-efekat određ en je u rasponu koncentracija efedrina od 0.0005 μM do 500 μM. Tri koncentracije efedrina (1, 50 and 300 μM) koje su imale prihvatljiv nivo ćelijske vijabilnosti (preko 90%) upotrebljene su za dalje eksperimente sa inhibitorima reparacije DNK (citozin arabinozid i hidroksiurea). Dobijeni rezultati pokazuju da efedrin nije indukovao oštećenja DNK na izolovanim limfocitima čoveka. Međutim, istovremeni tretman sa inhibitorima reparacije DNK doveo je do malog ali statistički značajnog porasta oštećenja DNK kod negativne kontrole, usled endogenog oštećenja DNK. Interestantno je da ćelije tretirane sa efedrinom i inhibitorima reparacije DNK nisu ispoljile povećan nivo oštećenja DNK. Na osnovu dobijenih rezultata može se zaključiti da efedrin nije ispoljio genotoksične efekte na izolovanim limfocitima čoveka. Ovaj rezultat je u saglasnosti sa prethodnim istraživanjima u kojima je dokazano da efedrin ne dovodi do genotoksičnih efekata u bakterijskim testovima na genske mutacije i u in vitro citogenetičkim analizama.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Evaluation of the effects of ephedrine on human lymphocytes in the comet assay
T1  - Evaluacija dejstva efedrina na limfocite čoveka u komet testu
VL  - 61
IS  - 4
SP  - 363
EP  - 371
DO  - 10.2298/AVB1104363R
ER  - 

@article{
author = "Radaković, Milena and Đelić, Ninoslav and Stanimirović, Zoran and Plećaš-Solarović, Bosiljka and Spremo-Potparević, Biljana and Živković, Lada and Bajić, Vladan",
year = "2011",
abstract = "Ephedrine, a natural alkaloid from plants of the genus Ephedra, has a chemical structure similar to catecholamines. It is well established that catecholamines (adraneline, noradrenaline and dopamine) cause genotoxic and mutagenic effects. Therefore, the objectives of this investigation were to examine weather ephedrine can exhibit genotoxic effects on isolated human lymphocytes in the Comet assay. Dose-response of human lymphocytes was determined at the concentration range of ephedrine from 0.0005 μM to 500 μM. Three concentrations of ephedrine (1, 50 and 300 μM) which had acceptable cell viability (over 90%) were used for further experiments with inhibitors of DNA reparation (cytosine arabinoside and hydroxyurea). The obtained results showed that ephedrine did not induce DNA damage in isolated human lymphocytes. However, co-treatment of the negative control with DNA repair inhibitors caused a slight but significant increase of DNA damage, due to an endogenous DNA damage. Interestingly, cells treated with ephedrine and DNA repair inhibitors did not express increased DNA damage. On the basis of the obtained results it can be concluded that ephedrine did not exhibit genotoxic effects on isolated human lymphocytes. This result is in accordance with previous investigations showing negative genotoxicological results for ephedrine using bacterial gene mutation test-systems and in vitro cytogenetic analysis., Efedrin, prirodni alkaloid iz biljaka roda Ephedra, ima sličnu hemijsku strukturu sa kateholaminima. Dobro je poznato da kateholamini (adrenalin, noradrenalin i dopamin) mogu da prouzrokuju genotoksične i mutagene efekte. Stoga su ciljevi ovog istraživanja bili da se ispita da li efedrin može da ispolji genotoksične efekte na izolovanim limfocitima čoveka u Komet testu. Odnos doza-efekat određ en je u rasponu koncentracija efedrina od 0.0005 μM do 500 μM. Tri koncentracije efedrina (1, 50 and 300 μM) koje su imale prihvatljiv nivo ćelijske vijabilnosti (preko 90%) upotrebljene su za dalje eksperimente sa inhibitorima reparacije DNK (citozin arabinozid i hidroksiurea). Dobijeni rezultati pokazuju da efedrin nije indukovao oštećenja DNK na izolovanim limfocitima čoveka. Međutim, istovremeni tretman sa inhibitorima reparacije DNK doveo je do malog ali statistički značajnog porasta oštećenja DNK kod negativne kontrole, usled endogenog oštećenja DNK. Interestantno je da ćelije tretirane sa efedrinom i inhibitorima reparacije DNK nisu ispoljile povećan nivo oštećenja DNK. Na osnovu dobijenih rezultata može se zaključiti da efedrin nije ispoljio genotoksične efekte na izolovanim limfocitima čoveka. Ovaj rezultat je u saglasnosti sa prethodnim istraživanjima u kojima je dokazano da efedrin ne dovodi do genotoksičnih efekata u bakterijskim testovima na genske mutacije i u in vitro citogenetičkim analizama.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Evaluation of the effects of ephedrine on human lymphocytes in the comet assay, Evaluacija dejstva efedrina na limfocite čoveka u komet testu",
volume = "61",
number = "4",
pages = "363-371",
doi = "10.2298/AVB1104363R"
}

Radaković, M., Đelić, N., Stanimirović, Z., Plećaš-Solarović, B., Spremo-Potparević, B., Živković, L.,& Bajić, V.. (2011). Evaluation of the effects of ephedrine on human lymphocytes in the comet assay. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(4), 363-371.
https://doi.org/10.2298/AVB1104363R

Radaković M, Đelić N, Stanimirović Z, Plećaš-Solarović B, Spremo-Potparević B, Živković L, Bajić V. Evaluation of the effects of ephedrine on human lymphocytes in the comet assay. in Acta veterinaria. 2011;61(4):363-371.
doi:10.2298/AVB1104363R .

Radaković, Milena, Đelić, Ninoslav, Stanimirović, Zoran, Plećaš-Solarović, Bosiljka, Spremo-Potparević, Biljana, Živković, Lada, Bajić, Vladan, "Evaluation of the effects of ephedrine on human lymphocytes in the comet assay" in Acta veterinaria, 61, no. 4 (2011):363-371,
https://doi.org/10.2298/AVB1104363R . .

TY  - JOUR
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Plećaš-Solarović, Bosiljka
AU  - Bajić, Vladan
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1615
AB  - Alzheimer's disease (AD), as the most common form of dementia, has for many years attracted the attention of researchers around the world, primarily because of the problems of reliable diagnostic methods that could help in the early detection of this devastating disease. One of the important aspects of genetic research related to AD is the analysis of chromosome instability which includes: aneuploidies of different chromosomes, telomere shortening and the phenomenon of premature centromere division (PCD). The aim of this study was to describe specific biomarkers in different types of cells as potential parameters for the diagnosis of AD in order to promptly recognize pre-symptomatic stages and prevent the development of disease and/or slow down its progression.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Chromosome instability in Alzheimer's disease
VL  - 63
IS  - 3
SP  - 603
EP  - 608
DO  - 10.2298/ABS1103603P
ER  - 

@article{
author = "Potparević, Biljana and Živković, Lada and Plećaš-Solarović, Bosiljka and Bajić, Vladan",
year = "2011",
abstract = "Alzheimer's disease (AD), as the most common form of dementia, has for many years attracted the attention of researchers around the world, primarily because of the problems of reliable diagnostic methods that could help in the early detection of this devastating disease. One of the important aspects of genetic research related to AD is the analysis of chromosome instability which includes: aneuploidies of different chromosomes, telomere shortening and the phenomenon of premature centromere division (PCD). The aim of this study was to describe specific biomarkers in different types of cells as potential parameters for the diagnosis of AD in order to promptly recognize pre-symptomatic stages and prevent the development of disease and/or slow down its progression.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Chromosome instability in Alzheimer's disease",
volume = "63",
number = "3",
pages = "603-608",
doi = "10.2298/ABS1103603P"
}

Potparević, B., Živković, L., Plećaš-Solarović, B.,& Bajić, V.. (2011). Chromosome instability in Alzheimer's disease. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(3), 603-608.
https://doi.org/10.2298/ABS1103603P

Potparević B, Živković L, Plećaš-Solarović B, Bajić V. Chromosome instability in Alzheimer's disease. in Archives of Biological Sciences. 2011;63(3):603-608.
doi:10.2298/ABS1103603P .

Potparević, Biljana, Živković, Lada, Plećaš-Solarović, Bosiljka, Bajić, Vladan, "Chromosome instability in Alzheimer's disease" in Archives of Biological Sciences, 63, no. 3 (2011):603-608,
https://doi.org/10.2298/ABS1103603P . .

TY  - JOUR
AU  - Živković, Lada
AU  - Potparević, Biljana
AU  - Plećaš-Solarović, Bosiljka
AU  - Đelić, Ninoslav
AU  - Ocić, Gordana
AU  - Smiljković, Predrag
AU  - Siedlak, Sandra L.
AU  - Smith, Mark A.
AU  - Bajić, Vladan
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1370
AB  - Chromosomal alterations are a feature of both aging and Alzheimer's disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.
PB  - Oxford Univ Press Inc, Cary
T2  - Journals of Gerontology Series A: Biological Sciences and Medical Sciences
T1  - Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age
VL  - 65
IS  - 12
SP  - 1269
EP  - 1274
DO  - 10.1093/gerona/glq148
ER  - 

@article{
author = "Živković, Lada and Potparević, Biljana and Plećaš-Solarović, Bosiljka and Đelić, Ninoslav and Ocić, Gordana and Smiljković, Predrag and Siedlak, Sandra L. and Smith, Mark A. and Bajić, Vladan",
year = "2010",
abstract = "Chromosomal alterations are a feature of both aging and Alzheimer's disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Journals of Gerontology Series A: Biological Sciences and Medical Sciences",
title = "Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age",
volume = "65",
number = "12",
pages = "1269-1274",
doi = "10.1093/gerona/glq148"
}

Živković, L., Potparević, B., Plećaš-Solarović, B., Đelić, N., Ocić, G., Smiljković, P., Siedlak, S. L., Smith, M. A.,& Bajić, V.. (2010). Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age. in Journals of Gerontology Series A: Biological Sciences and Medical Sciences
Oxford Univ Press Inc, Cary., 65(12), 1269-1274.
https://doi.org/10.1093/gerona/glq148

Živković L, Potparević B, Plećaš-Solarović B, Đelić N, Ocić G, Smiljković P, Siedlak SL, Smith MA, Bajić V. Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age. in Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2010;65(12):1269-1274.
doi:10.1093/gerona/glq148 .

Živković, Lada, Potparević, Biljana, Plećaš-Solarović, Bosiljka, Đelić, Ninoslav, Ocić, Gordana, Smiljković, Predrag, Siedlak, Sandra L., Smith, Mark A., Bajić, Vladan, "Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer's Disease Patients: Relation to Gender and Age" in Journals of Gerontology Series A: Biological Sciences and Medical Sciences, 65, no. 12 (2010):1269-1274,
https://doi.org/10.1093/gerona/glq148 . .

TY  - JOUR
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Plećaš-Solarović, Bosiljka
AU  - Smith, Mark A.
AU  - Bajić, Vladan
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1114
AB  - Premature centromere division (PCD) represents a loss of control over the sequential separation and segregation of chromosome centromeres. Although first described in aging women, PCD on the X chromosome (PCD,X) is markedly elevated in peripheral blood lymphocytes of individuals suffering from Alzheimer disease (AD). The present study evaluated PCD,X, using a fluorescent in situ hybridization method, in interphase nuclei of frontal cerebral cortex neurons from sporadic AD patients and age-matched controls. The average frequency of PCD,X in AD patients (8.60 +/- 1.20%) was almost three times higher (p  lt  0.01) than in the control group (2.96 +/- 1.20). However, consistent with previous studies, no mitotic cells were found in neurons in either AD or control brain, suggesting an intrinsic inability of post-mitotic neurons to divide. In view of the fact that it has been well-documented that neurons in AD can re-enter into the cell division cycle, the findings presented here of increased PCD advance the hypothesis that deregulation of the cell cycle may contribute to neuronal degeneration and subsequent cognitive deficits in AD.
PB  - Wiley-Blackwell, Malden
T2  - Journal of Neurochemistry
T1  - Premature centromere division of the X chromosome in neurons in Alzheimer's disease
VL  - 106
IS  - 5
SP  - 2218
EP  - 2223
DO  - 10.1111/j.1471-4159.2008.05555.x
ER  - 

@article{
author = "Potparević, Biljana and Živković, Lada and Đelić, Ninoslav and Plećaš-Solarović, Bosiljka and Smith, Mark A. and Bajić, Vladan",
year = "2008",
abstract = "Premature centromere division (PCD) represents a loss of control over the sequential separation and segregation of chromosome centromeres. Although first described in aging women, PCD on the X chromosome (PCD,X) is markedly elevated in peripheral blood lymphocytes of individuals suffering from Alzheimer disease (AD). The present study evaluated PCD,X, using a fluorescent in situ hybridization method, in interphase nuclei of frontal cerebral cortex neurons from sporadic AD patients and age-matched controls. The average frequency of PCD,X in AD patients (8.60 +/- 1.20%) was almost three times higher (p  lt  0.01) than in the control group (2.96 +/- 1.20). However, consistent with previous studies, no mitotic cells were found in neurons in either AD or control brain, suggesting an intrinsic inability of post-mitotic neurons to divide. In view of the fact that it has been well-documented that neurons in AD can re-enter into the cell division cycle, the findings presented here of increased PCD advance the hypothesis that deregulation of the cell cycle may contribute to neuronal degeneration and subsequent cognitive deficits in AD.",
publisher = "Wiley-Blackwell, Malden",
journal = "Journal of Neurochemistry",
title = "Premature centromere division of the X chromosome in neurons in Alzheimer's disease",
volume = "106",
number = "5",
pages = "2218-2223",
doi = "10.1111/j.1471-4159.2008.05555.x"
}

Potparević, B., Živković, L., Đelić, N., Plećaš-Solarović, B., Smith, M. A.,& Bajić, V.. (2008). Premature centromere division of the X chromosome in neurons in Alzheimer's disease. in Journal of Neurochemistry
Wiley-Blackwell, Malden., 106(5), 2218-2223.
https://doi.org/10.1111/j.1471-4159.2008.05555.x

Potparević B, Živković L, Đelić N, Plećaš-Solarović B, Smith MA, Bajić V. Premature centromere division of the X chromosome in neurons in Alzheimer's disease. in Journal of Neurochemistry. 2008;106(5):2218-2223.
doi:10.1111/j.1471-4159.2008.05555.x .

Potparević, Biljana, Živković, Lada, Đelić, Ninoslav, Plećaš-Solarović, Bosiljka, Smith, Mark A., Bajić, Vladan, "Premature centromere division of the X chromosome in neurons in Alzheimer's disease" in Journal of Neurochemistry, 106, no. 5 (2008):2218-2223,
https://doi.org/10.1111/j.1471-4159.2008.05555.x . .

TY  - JOUR
AU  - Pešić, V.
AU  - Radojević, Katarina
AU  - Kosec, Duško
AU  - Plećaš-Solarović, Bosiljka
AU  - Perišić, M.
AU  - Pilipović, Ivan
AU  - Leposavić, Gordana
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/987
AB  - The role of gonadal hormones in induction and, particularly, maintenance/ progression of rat thymic involution, which normally starts around puberty, was reassessed by examining the effects of peripubertal orchidectomy on thymic weight and morphometric parameters at different times up to the age of 10 months. Up to 6 months post-castration both thymic weight and cellularity in orchidectomized ( Cx) rats were greater than in age-matched control rats, sham Cx ( Sx). The increase in thymic cellularity reflected an increase in thymocyte proliferation rate ( the proportion of proliferating cells was 18.6 +/- 0.7% in 2-month-old Cx ( N = 5) vs 13.4 +/- 0.3% ( N = 5) in age-matched Sx rats) followed by reduced sensitivity to apoptotic signals ( apoptotic thymocytes were 9.8 +/- 0.9% in 2-month-old Cx ( N = 5) vs 15.5 +/- 0.3% ( N = 5) age-matched Sx rats). However, 9 months post-orchidectomy, neither thymic weight and cellularity nor any of the morphometric parameters analyzed differed between Cx and control rats. The reduction of thymic cellularity in Cx rats to control values may be related to increased sensitivity of their thymocytes to apoptotic signals in culture ( 72.6 +/- 1.2% in 10-month-old vs 9.8 +/- 0.9% in 2-month-old Cx rats) followed by reduced responsiveness to proliferative stimuli ( 14.1 +/- 0.2% in 10-month-old vs 18.6 +/- 0.7% in 2-month-old Cx rats). Thus, the study indicates that the effects of peripubertal orchidectomy on thymic weight and cellularity, as well as on the main morphometric indices, are long-lasting but not permanent, i.e., that removal of the testes can only postpone but not prevent age-related organ atrophy and consequently functional deterioration of the immune system.
PB  - Assoc Bras Divulg Cientifica, Sao Paulo
T2  - Brazilian Journal of Medical and Biological Research
T1  - Peripubertal orchidectomy transitorily affects age-associated thymic involution in rats
VL  - 40
IS  - 11
SP  - 1481
EP  - 1493
DO  - 10.1590/S0100-879X2006005000172
ER  - 

@article{
author = "Pešić, V. and Radojević, Katarina and Kosec, Duško and Plećaš-Solarović, Bosiljka and Perišić, M. and Pilipović, Ivan and Leposavić, Gordana",
year = "2007",
abstract = "The role of gonadal hormones in induction and, particularly, maintenance/ progression of rat thymic involution, which normally starts around puberty, was reassessed by examining the effects of peripubertal orchidectomy on thymic weight and morphometric parameters at different times up to the age of 10 months. Up to 6 months post-castration both thymic weight and cellularity in orchidectomized ( Cx) rats were greater than in age-matched control rats, sham Cx ( Sx). The increase in thymic cellularity reflected an increase in thymocyte proliferation rate ( the proportion of proliferating cells was 18.6 +/- 0.7% in 2-month-old Cx ( N = 5) vs 13.4 +/- 0.3% ( N = 5) in age-matched Sx rats) followed by reduced sensitivity to apoptotic signals ( apoptotic thymocytes were 9.8 +/- 0.9% in 2-month-old Cx ( N = 5) vs 15.5 +/- 0.3% ( N = 5) age-matched Sx rats). However, 9 months post-orchidectomy, neither thymic weight and cellularity nor any of the morphometric parameters analyzed differed between Cx and control rats. The reduction of thymic cellularity in Cx rats to control values may be related to increased sensitivity of their thymocytes to apoptotic signals in culture ( 72.6 +/- 1.2% in 10-month-old vs 9.8 +/- 0.9% in 2-month-old Cx rats) followed by reduced responsiveness to proliferative stimuli ( 14.1 +/- 0.2% in 10-month-old vs 18.6 +/- 0.7% in 2-month-old Cx rats). Thus, the study indicates that the effects of peripubertal orchidectomy on thymic weight and cellularity, as well as on the main morphometric indices, are long-lasting but not permanent, i.e., that removal of the testes can only postpone but not prevent age-related organ atrophy and consequently functional deterioration of the immune system.",
publisher = "Assoc Bras Divulg Cientifica, Sao Paulo",
journal = "Brazilian Journal of Medical and Biological Research",
title = "Peripubertal orchidectomy transitorily affects age-associated thymic involution in rats",
volume = "40",
number = "11",
pages = "1481-1493",
doi = "10.1590/S0100-879X2006005000172"
}

Pešić, V., Radojević, K., Kosec, D., Plećaš-Solarović, B., Perišić, M., Pilipović, I.,& Leposavić, G.. (2007). Peripubertal orchidectomy transitorily affects age-associated thymic involution in rats. in Brazilian Journal of Medical and Biological Research
Assoc Bras Divulg Cientifica, Sao Paulo., 40(11), 1481-1493.
https://doi.org/10.1590/S0100-879X2006005000172

Pešić V, Radojević K, Kosec D, Plećaš-Solarović B, Perišić M, Pilipović I, Leposavić G. Peripubertal orchidectomy transitorily affects age-associated thymic involution in rats. in Brazilian Journal of Medical and Biological Research. 2007;40(11):1481-1493.
doi:10.1590/S0100-879X2006005000172 .

Pešić, V., Radojević, Katarina, Kosec, Duško, Plećaš-Solarović, Bosiljka, Perišić, M., Pilipović, Ivan, Leposavić, Gordana, "Peripubertal orchidectomy transitorily affects age-associated thymic involution in rats" in Brazilian Journal of Medical and Biological Research, 40, no. 11 (2007):1481-1493,
https://doi.org/10.1590/S0100-879X2006005000172 . .

TY  - JOUR
AU  - Radojević, Katarina
AU  - Arsenović-Ranin, Nevena
AU  - Kosec, Duško
AU  - Pešić, V.
AU  - Pilipović, Ivan
AU  - Perišić, M.
AU  - Plećaš-Solarović, Bosiljka
AU  - Leposavić, Gordana
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/957
AB  - To test putative interdependence in the ontogenesis of the hypothalamic-pituitary-gonadal and thymic-lymphatic axes, thymocyte differentiation and maturation was examined in neonatally castrated (Cx) adult rats. In the hypercellular thymi of Cx rats, the proportion of the least mature CD4(-)CD8(-)TCR alpha beta(-) triple negative (TN) thymocytes was reduced, while the proportions of all downstream double positive (DP) subsets (TCR alpha beta(-), TCR alpha beta(low) and TCR alpha beta(high)) were increased when compared with neonatally sham-castrated (Sx) adult rats. This suggested an accelerated thymocyte transition from the TN to DP TCR alpha beta(low) developmental stage accompanied by an increased positive/reduced negative thymocyte selection. The increased thymocyte surface density of Thy-1, which is implicated in thymocyte hyposensitivity to negative selection, in Cx rats further supports the previous assumption. The finding that the proportions of both single positive (SP) TCR alpha beta(high) thymocyte subsets were reduced, while their numbers were increased (CD4(+)CD8(-)) or unaltered (CD4(-)CD8(+)), coupled with results demonstrating an increased level of CD4(-)CD8(+) cells without changes in that of CD4(+) 8(-) cells in the spleen indicate: (i) accelerated differentiation and maturation of the positively selected DP TCR alpha beta(high) thymocytes towards CD4(-)8(+) TCR alpha beta(high) cells followed by increased emigration of the mature cells and (ii) decelerated hi h differentiation and maturation towards CD4(+)8(-) TCR alpha beta(high) cells in Cx rats. Furthermore, the unaltered proportion of intrathymically developing CD4(+)CD25(+)Foxp3(+) regulatory cells in Cx rats, in light of putative hyposensitivity of thymocytes to negative selection suggesting reduced elimination of autoreactive cells, may provide a firm basis for understanding the reasons behind increased susceptibility of Cx rats to autoimmune disease induction.
PB  - Bioscientifica Ltd, Bristol
T2  - Journal of Endocrinology
T1  - Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level
VL  - 192
IS  - 3
SP  - 669
EP  - 682
DO  - 10.1677/joe.1.07019
ER  - 

@article{
author = "Radojević, Katarina and Arsenović-Ranin, Nevena and Kosec, Duško and Pešić, V. and Pilipović, Ivan and Perišić, M. and Plećaš-Solarović, Bosiljka and Leposavić, Gordana",
year = "2007",
abstract = "To test putative interdependence in the ontogenesis of the hypothalamic-pituitary-gonadal and thymic-lymphatic axes, thymocyte differentiation and maturation was examined in neonatally castrated (Cx) adult rats. In the hypercellular thymi of Cx rats, the proportion of the least mature CD4(-)CD8(-)TCR alpha beta(-) triple negative (TN) thymocytes was reduced, while the proportions of all downstream double positive (DP) subsets (TCR alpha beta(-), TCR alpha beta(low) and TCR alpha beta(high)) were increased when compared with neonatally sham-castrated (Sx) adult rats. This suggested an accelerated thymocyte transition from the TN to DP TCR alpha beta(low) developmental stage accompanied by an increased positive/reduced negative thymocyte selection. The increased thymocyte surface density of Thy-1, which is implicated in thymocyte hyposensitivity to negative selection, in Cx rats further supports the previous assumption. The finding that the proportions of both single positive (SP) TCR alpha beta(high) thymocyte subsets were reduced, while their numbers were increased (CD4(+)CD8(-)) or unaltered (CD4(-)CD8(+)), coupled with results demonstrating an increased level of CD4(-)CD8(+) cells without changes in that of CD4(+) 8(-) cells in the spleen indicate: (i) accelerated differentiation and maturation of the positively selected DP TCR alpha beta(high) thymocytes towards CD4(-)8(+) TCR alpha beta(high) cells followed by increased emigration of the mature cells and (ii) decelerated hi h differentiation and maturation towards CD4(+)8(-) TCR alpha beta(high) cells in Cx rats. Furthermore, the unaltered proportion of intrathymically developing CD4(+)CD25(+)Foxp3(+) regulatory cells in Cx rats, in light of putative hyposensitivity of thymocytes to negative selection suggesting reduced elimination of autoreactive cells, may provide a firm basis for understanding the reasons behind increased susceptibility of Cx rats to autoimmune disease induction.",
publisher = "Bioscientifica Ltd, Bristol",
journal = "Journal of Endocrinology",
title = "Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level",
volume = "192",
number = "3",
pages = "669-682",
doi = "10.1677/joe.1.07019"
}

Radojević, K., Arsenović-Ranin, N., Kosec, D., Pešić, V., Pilipović, I., Perišić, M., Plećaš-Solarović, B.,& Leposavić, G.. (2007). Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level. in Journal of Endocrinology
Bioscientifica Ltd, Bristol., 192(3), 669-682.
https://doi.org/10.1677/joe.1.07019

Radojević K, Arsenović-Ranin N, Kosec D, Pešić V, Pilipović I, Perišić M, Plećaš-Solarović B, Leposavić G. Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level. in Journal of Endocrinology. 2007;192(3):669-682.
doi:10.1677/joe.1.07019 .

Radojević, Katarina, Arsenović-Ranin, Nevena, Kosec, Duško, Pešić, V., Pilipović, Ivan, Perišić, M., Plećaš-Solarović, Bosiljka, Leposavić, Gordana, "Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level" in Journal of Endocrinology, 192, no. 3 (2007):669-682,
https://doi.org/10.1677/joe.1.07019 . .

TY  - JOUR
AU  - Pešić, V.
AU  - Plećaš-Solarović, Bosiljka
AU  - Radojević, Katarina
AU  - Kosec, Duško
AU  - Pilipović, Ivan
AU  - Perišić, M.
AU  - Leposavić, Gordana
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/916
AB  - Age-related increase in the density of thymic noradrenergic fibres and noradrenaline (NA) concentration is proposed to be associated with thymic involution and altered thymopoiesis. To test this hypothesis thymocyte differentiation/maturation and thymic structure were studied in 18-month-old male Wistar rats subjected to 14-day-long propranolol (P) blockade of ss-adrenoceptors (ss-ARs). The treatment primarily resulted in changes in the T-cell receptor (TCR)-dependent stages of thymopoiesis, which led to an increase in both the relative and absolute numbers of the most mature single positive (SP) CD4(+)CD8(-) (including cells with the CD4(+)CD25(+) regulatory phenotype) and CD4(-)CD8(+) TCR alpha ss(high) thymocytes. Accordingly, in the thymi of these rats an increase in both numerical density and absolute number of medullary thymocytes encompassing mainly the most mature SP cells was found. These findings, together with an increase in the thymocyte surface expression of the regulatory molecule Thy-1 (CD90) (implicated in negative regulation of TCR alpha beta-dependent thymocyte selection thresholds) in the same rats, may suggest increased positive/reduced negative thymocyte selection. Collectively, the results indicate that a decline in thymic efficiency in generating both conventional and regulatory T cells, and consequently in immune function, in aged rats may be, at least partly, attenuated by long-term blockade of beta-ARs with P.
PB  - Elsevier Science BV, Amsterdam
T2  - International Immunopharmacology
T1  - Long-term beta-adrenergic receptor blockade increases levels of the most mature thymocyte subsets in aged rats
VL  - 7
IS  - 5
SP  - 674
EP  - 686
DO  - 10.1016/j.intimp.2007.01.017
ER  - 

@article{
author = "Pešić, V. and Plećaš-Solarović, Bosiljka and Radojević, Katarina and Kosec, Duško and Pilipović, Ivan and Perišić, M. and Leposavić, Gordana",
year = "2007",
abstract = "Age-related increase in the density of thymic noradrenergic fibres and noradrenaline (NA) concentration is proposed to be associated with thymic involution and altered thymopoiesis. To test this hypothesis thymocyte differentiation/maturation and thymic structure were studied in 18-month-old male Wistar rats subjected to 14-day-long propranolol (P) blockade of ss-adrenoceptors (ss-ARs). The treatment primarily resulted in changes in the T-cell receptor (TCR)-dependent stages of thymopoiesis, which led to an increase in both the relative and absolute numbers of the most mature single positive (SP) CD4(+)CD8(-) (including cells with the CD4(+)CD25(+) regulatory phenotype) and CD4(-)CD8(+) TCR alpha ss(high) thymocytes. Accordingly, in the thymi of these rats an increase in both numerical density and absolute number of medullary thymocytes encompassing mainly the most mature SP cells was found. These findings, together with an increase in the thymocyte surface expression of the regulatory molecule Thy-1 (CD90) (implicated in negative regulation of TCR alpha beta-dependent thymocyte selection thresholds) in the same rats, may suggest increased positive/reduced negative thymocyte selection. Collectively, the results indicate that a decline in thymic efficiency in generating both conventional and regulatory T cells, and consequently in immune function, in aged rats may be, at least partly, attenuated by long-term blockade of beta-ARs with P.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Immunopharmacology",
title = "Long-term beta-adrenergic receptor blockade increases levels of the most mature thymocyte subsets in aged rats",
volume = "7",
number = "5",
pages = "674-686",
doi = "10.1016/j.intimp.2007.01.017"
}

Pešić, V., Plećaš-Solarović, B., Radojević, K., Kosec, D., Pilipović, I., Perišić, M.,& Leposavić, G.. (2007). Long-term beta-adrenergic receptor blockade increases levels of the most mature thymocyte subsets in aged rats. in International Immunopharmacology
Elsevier Science BV, Amsterdam., 7(5), 674-686.
https://doi.org/10.1016/j.intimp.2007.01.017

Pešić V, Plećaš-Solarović B, Radojević K, Kosec D, Pilipović I, Perišić M, Leposavić G. Long-term beta-adrenergic receptor blockade increases levels of the most mature thymocyte subsets in aged rats. in International Immunopharmacology. 2007;7(5):674-686.
doi:10.1016/j.intimp.2007.01.017 .

Pešić, V., Plećaš-Solarović, Bosiljka, Radojević, Katarina, Kosec, Duško, Pilipović, Ivan, Perišić, M., Leposavić, Gordana, "Long-term beta-adrenergic receptor blockade increases levels of the most mature thymocyte subsets in aged rats" in International Immunopharmacology, 7, no. 5 (2007):674-686,
https://doi.org/10.1016/j.intimp.2007.01.017 . .

TY  - JOUR
AU  - Leposavić, Gordana
AU  - Pešić, Vesna
AU  - Kosec, Duško
AU  - Radojević, Katarina
AU  - Arsenović-Ranin, Nevena
AU  - Pilipović, Ivan
AU  - Perišić, Milica
AU  - Plećaš-Solarović, Bosiljka
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/816
AB  - To elucidate the effects of ageing on T-cell-maturation, in 3- and 18-month-old rats, we analysed the expression of: (i) CD4/CD8/TCR alpha beta and (ii) Thy-1, which is supposed to be a regulator of TCR alpha beta signalling, and thereby the thymocyte selection thresholds. Since an essential role for TCR alpha beta signalling in the development of CD4+25+T-reg-cells was suggested, the frequency of these cells was also quantified. We demonstrated that, as for mice, early thymocyte differentiational steps within the CD4-8- double negative (DN) developmental stage are age-sensitive. Furthermore, we revealed that TCRao-dependent stages of T-cell development are affected by ageing, most likely due to an impaired expression of Thy-1 on TCR alpha beta(low) thymocytes entering selection processes. The diminished frequency of the post-selection CD4+8+ double positive (DP) cells in aged rats, together with an overrepresentation of mature single positive (SP) cells, most probably suggests more efficient differentiational transition from the DP TCR alpha beta(high) to the SP TCR alpha beta(high) developmental stage, which is followed by an increase in pre-migration proliferation of the mature SP cells. Moreover, the study indicated impaired intrathymic generation of CD4+25+T-reg-cells in aged rats, thus providing a possible explanation for the increased frequency of autoimmune diseases in ageing.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Experimental Gerontology
T1  - Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats
VL  - 41
IS  - 6
SP  - 574
EP  - 589
DO  - 10.1016/j.exger.2006.03.006
ER  - 

@article{
author = "Leposavić, Gordana and Pešić, Vesna and Kosec, Duško and Radojević, Katarina and Arsenović-Ranin, Nevena and Pilipović, Ivan and Perišić, Milica and Plećaš-Solarović, Bosiljka",
year = "2006",
abstract = "To elucidate the effects of ageing on T-cell-maturation, in 3- and 18-month-old rats, we analysed the expression of: (i) CD4/CD8/TCR alpha beta and (ii) Thy-1, which is supposed to be a regulator of TCR alpha beta signalling, and thereby the thymocyte selection thresholds. Since an essential role for TCR alpha beta signalling in the development of CD4+25+T-reg-cells was suggested, the frequency of these cells was also quantified. We demonstrated that, as for mice, early thymocyte differentiational steps within the CD4-8- double negative (DN) developmental stage are age-sensitive. Furthermore, we revealed that TCRao-dependent stages of T-cell development are affected by ageing, most likely due to an impaired expression of Thy-1 on TCR alpha beta(low) thymocytes entering selection processes. The diminished frequency of the post-selection CD4+8+ double positive (DP) cells in aged rats, together with an overrepresentation of mature single positive (SP) cells, most probably suggests more efficient differentiational transition from the DP TCR alpha beta(high) to the SP TCR alpha beta(high) developmental stage, which is followed by an increase in pre-migration proliferation of the mature SP cells. Moreover, the study indicated impaired intrathymic generation of CD4+25+T-reg-cells in aged rats, thus providing a possible explanation for the increased frequency of autoimmune diseases in ageing.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Experimental Gerontology",
title = "Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats",
volume = "41",
number = "6",
pages = "574-589",
doi = "10.1016/j.exger.2006.03.006"
}

Leposavić, G., Pešić, V., Kosec, D., Radojević, K., Arsenović-Ranin, N., Pilipović, I., Perišić, M.,& Plećaš-Solarović, B.. (2006). Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats. in Experimental Gerontology
Pergamon-Elsevier Science Ltd, Oxford., 41(6), 574-589.
https://doi.org/10.1016/j.exger.2006.03.006

Leposavić G, Pešić V, Kosec D, Radojević K, Arsenović-Ranin N, Pilipović I, Perišić M, Plećaš-Solarović B. Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats. in Experimental Gerontology. 2006;41(6):574-589.
doi:10.1016/j.exger.2006.03.006 .

Leposavić, Gordana, Pešić, Vesna, Kosec, Duško, Radojević, Katarina, Arsenović-Ranin, Nevena, Pilipović, Ivan, Perišić, Milica, Plećaš-Solarović, Bosiljka, "Age-associated changes in CD90 expression on thymocytes and in TCR-dependent stages of thymocyte maturation in male rats" in Experimental Gerontology, 41, no. 6 (2006):574-589,
https://doi.org/10.1016/j.exger.2006.03.006 . .

TY  - JOUR
AU  - Leposavić, Gordana
AU  - Arsenović-Ranin, Nevena
AU  - Radojević, Katarina
AU  - Kosec, Duško
AU  - Pešić, V.
AU  - Vidić-Danković, Biljana
AU  - Plećaš-Solarović, Bosiljka
AU  - Pilipović, Ivan
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/856
AB  - Adult male Wistar rats were subjected to propranolol (P, 0.40 mg/100 g/day) or saline (S) administration (controls) over 14 days. The expression of major differentiation molecules on thymocytes and Thy-1 (CD90) molecules, which are shown to adjust thymocyte sensitivity to TCR alpha beta signaling, was studied. In addition, the sensitivity of thymocytes to induction of apoptosis and concanavalin A (Con A) signaling was estimated. The thymocytes from P-treated (PT) rats exhibited an increased sensitivity to induction of apoptosis, as well as to Con A stimulation. Furthermore, P treatment produced changes in the distribution of thymocyte subsets suggesting that more cells passed positive selection and further differentiated into mature CD4+ or CD8+ single positive (SP) TCR alpha beta(high) cells. These changes may, at least partly, be related to the markedly increased density of Thy-1 surface expression on TCR alpha beta(low) thymocytes from these rats. The increased frequency of cells expressing the CD4+25+ phenotype, which has been shown to be characteristic for regulatory cells in the thymus, may also indicate alterations in thymocyte selection following P treatment. Inasmuch as positive and negative selections play an important role in continuously reshaping the T-cell repertoire and maintaining tolerance, the hereby presented study suggests that pharmacological manipulations with beta-AR signaling, or chemically evoked alterations in catecholamine release, may interfere with the regulation of thymocyte selection, and consequently with the immune response.
PB  - Springer, Dordrecht
T2  - Molecular and Cellular Biochemistry
T1  - Characterization of thymocyte phenotypic alterations induced by long-lasting beta-adrenoceptor blockade in vivo and its effects on thymocyte proliferation and apoptosis
VL  - 285
IS  - 1-2
SP  - 87
EP  - 99
DO  - 10.1007/s11010-005-9059-5
ER  - 

@article{
author = "Leposavić, Gordana and Arsenović-Ranin, Nevena and Radojević, Katarina and Kosec, Duško and Pešić, V. and Vidić-Danković, Biljana and Plećaš-Solarović, Bosiljka and Pilipović, Ivan",
year = "2006",
abstract = "Adult male Wistar rats were subjected to propranolol (P, 0.40 mg/100 g/day) or saline (S) administration (controls) over 14 days. The expression of major differentiation molecules on thymocytes and Thy-1 (CD90) molecules, which are shown to adjust thymocyte sensitivity to TCR alpha beta signaling, was studied. In addition, the sensitivity of thymocytes to induction of apoptosis and concanavalin A (Con A) signaling was estimated. The thymocytes from P-treated (PT) rats exhibited an increased sensitivity to induction of apoptosis, as well as to Con A stimulation. Furthermore, P treatment produced changes in the distribution of thymocyte subsets suggesting that more cells passed positive selection and further differentiated into mature CD4+ or CD8+ single positive (SP) TCR alpha beta(high) cells. These changes may, at least partly, be related to the markedly increased density of Thy-1 surface expression on TCR alpha beta(low) thymocytes from these rats. The increased frequency of cells expressing the CD4+25+ phenotype, which has been shown to be characteristic for regulatory cells in the thymus, may also indicate alterations in thymocyte selection following P treatment. Inasmuch as positive and negative selections play an important role in continuously reshaping the T-cell repertoire and maintaining tolerance, the hereby presented study suggests that pharmacological manipulations with beta-AR signaling, or chemically evoked alterations in catecholamine release, may interfere with the regulation of thymocyte selection, and consequently with the immune response.",
publisher = "Springer, Dordrecht",
journal = "Molecular and Cellular Biochemistry",
title = "Characterization of thymocyte phenotypic alterations induced by long-lasting beta-adrenoceptor blockade in vivo and its effects on thymocyte proliferation and apoptosis",
volume = "285",
number = "1-2",
pages = "87-99",
doi = "10.1007/s11010-005-9059-5"
}

Leposavić, G., Arsenović-Ranin, N., Radojević, K., Kosec, D., Pešić, V., Vidić-Danković, B., Plećaš-Solarović, B.,& Pilipović, I.. (2006). Characterization of thymocyte phenotypic alterations induced by long-lasting beta-adrenoceptor blockade in vivo and its effects on thymocyte proliferation and apoptosis. in Molecular and Cellular Biochemistry
Springer, Dordrecht., 285(1-2), 87-99.
https://doi.org/10.1007/s11010-005-9059-5

Leposavić G, Arsenović-Ranin N, Radojević K, Kosec D, Pešić V, Vidić-Danković B, Plećaš-Solarović B, Pilipović I. Characterization of thymocyte phenotypic alterations induced by long-lasting beta-adrenoceptor blockade in vivo and its effects on thymocyte proliferation and apoptosis. in Molecular and Cellular Biochemistry. 2006;285(1-2):87-99.
doi:10.1007/s11010-005-9059-5 .

Leposavić, Gordana, Arsenović-Ranin, Nevena, Radojević, Katarina, Kosec, Duško, Pešić, V., Vidić-Danković, Biljana, Plećaš-Solarović, Bosiljka, Pilipović, Ivan, "Characterization of thymocyte phenotypic alterations induced by long-lasting beta-adrenoceptor blockade in vivo and its effects on thymocyte proliferation and apoptosis" in Molecular and Cellular Biochemistry, 285, no. 1-2 (2006):87-99,
https://doi.org/10.1007/s11010-005-9059-5 . .

TY  - JOUR
AU  - Plećaš-Solarović, Bosiljka
AU  - Pešić, V.
AU  - Radojević, Katarina
AU  - Leposavić, Gordana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/670
AB  - In order to provide a morphometrical description of the changes in the aged rat thymus and to relate them to apoptotic and proliferative activity of thymocytes, the thymuses from 3- and 18-month-old male Wistar rats and the percentages of bromodeoxyuridine-incorporating and apoptotic cells in cultures of thymocytes were assessed by stereological analysis and flow cytometry, respectively. In old rats the volume of lymphoepithelial thymic tissue is markedly reduced, reflecting a sharp decrease in the total number of thymocytes. A reduction in the proliferative capacity of thymocytes and increase in their susceptibility to apoptosis are, most likely, primarily responsible for a 7-fold reduction in thymic cellularity in old animals. Furthermore, only the volume of cortical compartment was affected by aging, while that of medulla, despite of reduced cellularity, was not significantly altered. The loss of functional tissue in aged thymus is compensated by a substantial increase in the volume of inter-lobular connective and adipose tissue, so the thymic weight remained unaltered in old rats. These results suggest that thymus of aged Wistar rats exhibits morphological characteristics similar to those found in aged human thymus and thus may serve as an animal model for further investigations of thymus-related changes in immunological aging.
PB  - Wiley, Hoboken
T2  - Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia
T1  - Morphometrical characteristics of age-associated changes in the thymus of old male wistar rats
VL  - 35
IS  - 6
SP  - 380
EP  - 386
DO  - 10.1111/j.1439-0264.2006.00698.x
ER  - 

@article{
author = "Plećaš-Solarović, Bosiljka and Pešić, V. and Radojević, Katarina and Leposavić, Gordana",
year = "2006",
abstract = "In order to provide a morphometrical description of the changes in the aged rat thymus and to relate them to apoptotic and proliferative activity of thymocytes, the thymuses from 3- and 18-month-old male Wistar rats and the percentages of bromodeoxyuridine-incorporating and apoptotic cells in cultures of thymocytes were assessed by stereological analysis and flow cytometry, respectively. In old rats the volume of lymphoepithelial thymic tissue is markedly reduced, reflecting a sharp decrease in the total number of thymocytes. A reduction in the proliferative capacity of thymocytes and increase in their susceptibility to apoptosis are, most likely, primarily responsible for a 7-fold reduction in thymic cellularity in old animals. Furthermore, only the volume of cortical compartment was affected by aging, while that of medulla, despite of reduced cellularity, was not significantly altered. The loss of functional tissue in aged thymus is compensated by a substantial increase in the volume of inter-lobular connective and adipose tissue, so the thymic weight remained unaltered in old rats. These results suggest that thymus of aged Wistar rats exhibits morphological characteristics similar to those found in aged human thymus and thus may serve as an animal model for further investigations of thymus-related changes in immunological aging.",
publisher = "Wiley, Hoboken",
journal = "Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia",
title = "Morphometrical characteristics of age-associated changes in the thymus of old male wistar rats",
volume = "35",
number = "6",
pages = "380-386",
doi = "10.1111/j.1439-0264.2006.00698.x"
}

Plećaš-Solarović, B., Pešić, V., Radojević, K.,& Leposavić, G.. (2006). Morphometrical characteristics of age-associated changes in the thymus of old male wistar rats. in Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia
Wiley, Hoboken., 35(6), 380-386.
https://doi.org/10.1111/j.1439-0264.2006.00698.x

Plećaš-Solarović B, Pešić V, Radojević K, Leposavić G. Morphometrical characteristics of age-associated changes in the thymus of old male wistar rats. in Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia. 2006;35(6):380-386.
doi:10.1111/j.1439-0264.2006.00698.x .

Plećaš-Solarović, Bosiljka, Pešić, V., Radojević, Katarina, Leposavić, Gordana, "Morphometrical characteristics of age-associated changes in the thymus of old male wistar rats" in Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia, 35, no. 6 (2006):380-386,
https://doi.org/10.1111/j.1439-0264.2006.00698.x . .

TY  - JOUR
AU  - Plećaš-Solarović, Bosiljka
AU  - Leposavić, Gordana
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/582
AB  - Morphometric characteristics of adrenal medulla were analysed stereologically in adult male rats injected with a single dose of ethane dirnethanesulphonate (EDS), an agent that causes atrophy of the inner adrenocortical zone, or vehicle, and subjected to isoproterenol (ISO) or propranolol (PROP) treatment over the following 15 days. Compared with dimethylsulphoxide (DMSO) vehicle-injected controls, in EDS-administered rats the volume of chromaffin cell nuclei was decreased. ISO treatment in these rats increased the volumes of chromaffin cells and their nuclei. Furthermore, in both EDS- and vehicle-injected rats ISO significantly enlarged the total volume of medullary blood vessels, suggesting a vasodilatatory effect of beta-adrenoceptor stimulation. However, unexpectedly, in EDS-injected rats PROP treatment also caused an increase in the volumes of chromaffin cells and their nuclei. This finding, most likely, may be related to a non-beta-adrenoceptor-related action of PROP. Collectively, the present results suggest that the response of adrenomedullary chromaffin cells to chronic ISO-induced P-adrenoceptor stimulation is dependent on the functional status of adrenal cortex, so that the stereologically detectable changes were found only in rats previously exposed to the destructive action of EDS on the adrenal gland cortical cells. Moreover, they indicate that ISO treatment exerts a reversing effect on the morphometrical changes of chromaffine cells induced by EDS administration.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Experimental Animal Science
T1  - Morphometric changes in the adrenal medulla of intact and ethane dimethanesulphonate (EDS)-injected rats subjected to chronic treatment with isoproterenol or propranolol
VL  - 43
IS  - 2
SP  - 117
EP  - 125
DO  - 10.1016/j.jeas.2005.02.001
ER  - 

@article{
author = "Plećaš-Solarović, Bosiljka and Leposavić, Gordana",
year = "2005",
abstract = "Morphometric characteristics of adrenal medulla were analysed stereologically in adult male rats injected with a single dose of ethane dirnethanesulphonate (EDS), an agent that causes atrophy of the inner adrenocortical zone, or vehicle, and subjected to isoproterenol (ISO) or propranolol (PROP) treatment over the following 15 days. Compared with dimethylsulphoxide (DMSO) vehicle-injected controls, in EDS-administered rats the volume of chromaffin cell nuclei was decreased. ISO treatment in these rats increased the volumes of chromaffin cells and their nuclei. Furthermore, in both EDS- and vehicle-injected rats ISO significantly enlarged the total volume of medullary blood vessels, suggesting a vasodilatatory effect of beta-adrenoceptor stimulation. However, unexpectedly, in EDS-injected rats PROP treatment also caused an increase in the volumes of chromaffin cells and their nuclei. This finding, most likely, may be related to a non-beta-adrenoceptor-related action of PROP. Collectively, the present results suggest that the response of adrenomedullary chromaffin cells to chronic ISO-induced P-adrenoceptor stimulation is dependent on the functional status of adrenal cortex, so that the stereologically detectable changes were found only in rats previously exposed to the destructive action of EDS on the adrenal gland cortical cells. Moreover, they indicate that ISO treatment exerts a reversing effect on the morphometrical changes of chromaffine cells induced by EDS administration.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Experimental Animal Science",
title = "Morphometric changes in the adrenal medulla of intact and ethane dimethanesulphonate (EDS)-injected rats subjected to chronic treatment with isoproterenol or propranolol",
volume = "43",
number = "2",
pages = "117-125",
doi = "10.1016/j.jeas.2005.02.001"
}

Plećaš-Solarović, B.,& Leposavić, G.. (2005). Morphometric changes in the adrenal medulla of intact and ethane dimethanesulphonate (EDS)-injected rats subjected to chronic treatment with isoproterenol or propranolol. in Journal of Experimental Animal Science
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 43(2), 117-125.
https://doi.org/10.1016/j.jeas.2005.02.001

Plećaš-Solarović B, Leposavić G. Morphometric changes in the adrenal medulla of intact and ethane dimethanesulphonate (EDS)-injected rats subjected to chronic treatment with isoproterenol or propranolol. in Journal of Experimental Animal Science. 2005;43(2):117-125.
doi:10.1016/j.jeas.2005.02.001 .

Plećaš-Solarović, Bosiljka, Leposavić, Gordana, "Morphometric changes in the adrenal medulla of intact and ethane dimethanesulphonate (EDS)-injected rats subjected to chronic treatment with isoproterenol or propranolol" in Journal of Experimental Animal Science, 43, no. 2 (2005):117-125,
https://doi.org/10.1016/j.jeas.2005.02.001 . .

TY  - JOUR
AU  - Plećaš-Solarović, Bosiljka
AU  - Hristić-Živković, I
AU  - Radojević, Katarina
AU  - Kosec, Duško
AU  - Leposavić, Gordana
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/578
AB  - In order to examine the influence of chronic alpha(1)-adrenergic receptor (alpha(1)-AR) blockade on the thymus structure and T-cell maturation, peripubertal and adult male rats were treated with urapidil (0.20 mg/kg BW/d; s.c.) over 15 consecutive days. Thymic structure and phenotypic characteristics of the thymocytes were assessed by stereological and flow cytometry analysis, respectively. In immature rats, treatment with urapidil reduced the body weight gain and, affecting the volume of cortical compartment and its cellularity decreased the organ size and the total number of thymocytes compared to acre-matched saline-injected controls. The percentage of CD4+8- single positive (SP) thymocytes was decreased, while that of CD4-8+ was increased suggesting, most likely, a disregulation in final steps of the positively selected cells maturation. However alpha(1)-AR blockade in adult rats increased the thymus weight as a consequence of increase in the cortical size and cellularity. The increased percentage of most immature CD4-8- double negative (DN) cells associated with decreased percentage of immature CD4+8+ double positive (DP) thymocytes suggests a decelerated transition from DN to DP stage of T-cell development. As in immature rats, the treatment in adult rats evoked changes in the relative numbers of SP cells, but contrary to immature animals, favoring the maturation of CD4+8- over CD4-8+ thymocytes. These results demonstrate that: i) chronic blockade of alpha(1)-ARs affects both the thymus structure and thymocyte differentiation, ii) these effects are age-dependent, pointing out to pharmacological manipulation of alpha(1)-AR-mediated signaling as potential means for modulation of the intrathymic T-cell maturation.
PB  - F Hernandez, Murcia
T2  - Histology and Histopathology
T1  - Chronic alpha(1)-adrenoreceptor blockade produces age-dependent changes in rat thymus structure and thymocyte differentiation
VL  - 20
IS  - 3
SP  - 833
EP  - 841
UR  - https://hdl.handle.net/21.15107/rcub_farfar_578
ER  - 

@article{
author = "Plećaš-Solarović, Bosiljka and Hristić-Živković, I and Radojević, Katarina and Kosec, Duško and Leposavić, Gordana",
year = "2005",
abstract = "In order to examine the influence of chronic alpha(1)-adrenergic receptor (alpha(1)-AR) blockade on the thymus structure and T-cell maturation, peripubertal and adult male rats were treated with urapidil (0.20 mg/kg BW/d; s.c.) over 15 consecutive days. Thymic structure and phenotypic characteristics of the thymocytes were assessed by stereological and flow cytometry analysis, respectively. In immature rats, treatment with urapidil reduced the body weight gain and, affecting the volume of cortical compartment and its cellularity decreased the organ size and the total number of thymocytes compared to acre-matched saline-injected controls. The percentage of CD4+8- single positive (SP) thymocytes was decreased, while that of CD4-8+ was increased suggesting, most likely, a disregulation in final steps of the positively selected cells maturation. However alpha(1)-AR blockade in adult rats increased the thymus weight as a consequence of increase in the cortical size and cellularity. The increased percentage of most immature CD4-8- double negative (DN) cells associated with decreased percentage of immature CD4+8+ double positive (DP) thymocytes suggests a decelerated transition from DN to DP stage of T-cell development. As in immature rats, the treatment in adult rats evoked changes in the relative numbers of SP cells, but contrary to immature animals, favoring the maturation of CD4+8- over CD4-8+ thymocytes. These results demonstrate that: i) chronic blockade of alpha(1)-ARs affects both the thymus structure and thymocyte differentiation, ii) these effects are age-dependent, pointing out to pharmacological manipulation of alpha(1)-AR-mediated signaling as potential means for modulation of the intrathymic T-cell maturation.",
publisher = "F Hernandez, Murcia",
journal = "Histology and Histopathology",
title = "Chronic alpha(1)-adrenoreceptor blockade produces age-dependent changes in rat thymus structure and thymocyte differentiation",
volume = "20",
number = "3",
pages = "833-841",
url = "https://hdl.handle.net/21.15107/rcub_farfar_578"
}

Plećaš-Solarović, B., Hristić-Živković, I., Radojević, K., Kosec, D.,& Leposavić, G.. (2005). Chronic alpha(1)-adrenoreceptor blockade produces age-dependent changes in rat thymus structure and thymocyte differentiation. in Histology and Histopathology
F Hernandez, Murcia., 20(3), 833-841.
https://hdl.handle.net/21.15107/rcub_farfar_578

Plećaš-Solarović B, Hristić-Živković I, Radojević K, Kosec D, Leposavić G. Chronic alpha(1)-adrenoreceptor blockade produces age-dependent changes in rat thymus structure and thymocyte differentiation. in Histology and Histopathology. 2005;20(3):833-841.
https://hdl.handle.net/21.15107/rcub_farfar_578 .

Plećaš-Solarović, Bosiljka, Hristić-Živković, I, Radojević, Katarina, Kosec, Duško, Leposavić, Gordana, "Chronic alpha(1)-adrenoreceptor blockade produces age-dependent changes in rat thymus structure and thymocyte differentiation" in Histology and Histopathology, 20, no. 3 (2005):833-841,
https://hdl.handle.net/21.15107/rcub_farfar_578 .

TY  - JOUR
AU  - Plećaš-Solarović, Bosiljka
AU  - Lalić, L
AU  - Leposavić, Gordana
PY  - 2004
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/490
AB  - In order to elucidate a putative role of neurally derived noradrenaline in the thymus development, and in maintenance of adult thymus structure, sexually immature male rats (21-day-old at the beginning of treatment) and young adult animals (75-day-old on the beginning of treatment) were treated with the non-selective beta-adrenoceptor antagonist propranolol (0.40 mg/100 g BW/day, s.c.) for 15 consecutive days, and their thymuses were analyzed stereologically. The effects of beta-adrenoceptor blockade were much more pronounced in sexually immature than in adult rats. In immature propranolol-treated rats the thymus size and volumes of both the main compartments (cortex and medulla) were significantly decreased reflecting, at least partly, a reduction in the overall number of thymocytes. Furthermore, in both the cortical subcompartments (outer and deep cortex) the mean diameter of thymocytes was increased. However, in adult rats exposed to propranolol treatment, only the volume of interlobular connective tissue was enlarged, whereas in the outer part of the cortex the mean thymocyte diameter was increased. These results indicate that the lack of sympathetic input (via beta-adrenoceptors) during the prepubertal period of development diminishes the normal thymus growth and/or accelerates the thymic involution that starts at puberty, immediately after its maximum size is reached, while it is less significant for the maintenance of the thymus size and structure in adults. Additionally, they suggest that distinct cell types, as well as thymocyte subsets, are sensitive to lack of beta-adrenoceptor-mediated influences in sexually immature and adult rats.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Annals of Anatomy - Anatomischer Anzeiger
T1  - Age-dependent morphometrical changes in the thymus of male propranolol-treated rats
VL  - 186
IS  - 2
SP  - 141
EP  - 147
DO  - 10.1016/S0940-9602(04)80028-1
ER  - 

@article{
author = "Plećaš-Solarović, Bosiljka and Lalić, L and Leposavić, Gordana",
year = "2004",
abstract = "In order to elucidate a putative role of neurally derived noradrenaline in the thymus development, and in maintenance of adult thymus structure, sexually immature male rats (21-day-old at the beginning of treatment) and young adult animals (75-day-old on the beginning of treatment) were treated with the non-selective beta-adrenoceptor antagonist propranolol (0.40 mg/100 g BW/day, s.c.) for 15 consecutive days, and their thymuses were analyzed stereologically. The effects of beta-adrenoceptor blockade were much more pronounced in sexually immature than in adult rats. In immature propranolol-treated rats the thymus size and volumes of both the main compartments (cortex and medulla) were significantly decreased reflecting, at least partly, a reduction in the overall number of thymocytes. Furthermore, in both the cortical subcompartments (outer and deep cortex) the mean diameter of thymocytes was increased. However, in adult rats exposed to propranolol treatment, only the volume of interlobular connective tissue was enlarged, whereas in the outer part of the cortex the mean thymocyte diameter was increased. These results indicate that the lack of sympathetic input (via beta-adrenoceptors) during the prepubertal period of development diminishes the normal thymus growth and/or accelerates the thymic involution that starts at puberty, immediately after its maximum size is reached, while it is less significant for the maintenance of the thymus size and structure in adults. Additionally, they suggest that distinct cell types, as well as thymocyte subsets, are sensitive to lack of beta-adrenoceptor-mediated influences in sexually immature and adult rats.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Annals of Anatomy - Anatomischer Anzeiger",
title = "Age-dependent morphometrical changes in the thymus of male propranolol-treated rats",
volume = "186",
number = "2",
pages = "141-147",
doi = "10.1016/S0940-9602(04)80028-1"
}

Plećaš-Solarović, B., Lalić, L.,& Leposavić, G.. (2004). Age-dependent morphometrical changes in the thymus of male propranolol-treated rats. in Annals of Anatomy - Anatomischer Anzeiger
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 186(2), 141-147.
https://doi.org/10.1016/S0940-9602(04)80028-1

Plećaš-Solarović B, Lalić L, Leposavić G. Age-dependent morphometrical changes in the thymus of male propranolol-treated rats. in Annals of Anatomy - Anatomischer Anzeiger. 2004;186(2):141-147.
doi:10.1016/S0940-9602(04)80028-1 .

Plećaš-Solarović, Bosiljka, Lalić, L, Leposavić, Gordana, "Age-dependent morphometrical changes in the thymus of male propranolol-treated rats" in Annals of Anatomy - Anatomischer Anzeiger, 186, no. 2 (2004):141-147,
https://doi.org/10.1016/S0940-9602(04)80028-1 . .

TY  - JOUR
AU  - Plećaš-Solarović, Bosiljka
AU  - Pešić, Vesna
AU  - Mirković, Duško
AU  - Leposavić, Gordana
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/468
AB  - Ethane dimethanesulphonate (EDS), an alkylating agent, caused marked atrophy of the adrenal cortex of adult male rats, in addition to its toxic effect on testicular Leydig cells. The aim of this work was to examine whether a 9-day treatment with ACTH (40 IU/kg/d), isoproterenol (120 mg/kg/d) or dexamethasone (0.25 mg/kg/d), which started 4 days prior to intraperitoneal administration of a single dose of EDS (75 mg/kg), affected the morphological changes in the adrenal cortex evoked by EDS alone. The animals were killed 15 days after EDS injection. Stereological analysis revealed that both ACTH and isoproterenol almost completely prevented cortical atrophy induced by EDS. They also considerably stimulated corticosterone secretion in EDS-injected animals. By contrast, in dexamethasone-suppressed rats, the deleterious effect of EDS on adrenocortical cells was augmented. The volume and cellularity of all cortical zones were reduced, but the remaining cells of the zona reticularis displayed considerable hypertrophy which was probably responsible for the maintenance of corticosterone secretion. These results clearly demonstrate that both ACTH and b adrenoceptor stimulation have protective action against the toxic effects of EDS on rat adrenal cortex, whereas dexamethasone exerts an opposite influence.
AB  - Alkilirajući agens etandimetansulfonat (EDS), pored toksičnog delovanja na Lajdigove ćelije semenika, izaziva izraženu atrofiju kore nadbubrežne žlezde odraslih pacova. U ovom radu je ispitivan uticaj devetodnevnog tretmana sa ACTH (40 IU/kg/d), izoproterenolom (120 mg/kg/d) ili deksametazonom (0.25 mg/kg/d) na morfološke promene kore nadbubrega koje izaziva jedna intraperitonealna injekcija EDS (75 mg/kg). Tretmani su započeli 4 dana pre davanja EDS i nastavljeni su još 5 dana, a životinje su žrtvovane 15 dana posle aplikacije EDS. U prisustvu ACTH ili izoproterenola ne ispoljavaju se promene u stereološkim parametrima kore nadbubrega koje izaziva EDS. Oba tretmana značajno povećavaju i koncentraciju kortikosterona u serumu. Deksametazon, međutim, pojačava toksične efekte EDS; zapremina svih zona kore i broj parenhimskih ćelija u zonama su smanjeni, ali preostale ćelije retikularne zone pokazuju značajnu hipertrofiju, koja je verovatno odgovorna za održavanje sekrecije kortikosterona kod ovih životinja. Rezultati rada pokazuju da se toksični efekti EDS na koru nadbubrega mogu sprečiti visokim nivoom ACTH ili stimulacijom b adrenalinskih receptora, a potencirati egzogenim glukokortikoidom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - The effects of acth, isoproterenol and Dexamethasone on the rat adrenal gland response to ethane dimethanesulphonate (Eds): A stereological study
T1  - Uticaj ACTH, izoproterenola i deksametazona na odgovor nadbubrežne žlezde pacova na etandimetansulfonat (EDS): stereološko ispitivanje
VL  - 53
IS  - 5-6
SP  - 333
EP  - 342
DO  - 10.2298/AVB0306333P
ER  - 

@article{
author = "Plećaš-Solarović, Bosiljka and Pešić, Vesna and Mirković, Duško and Leposavić, Gordana",
year = "2003",
abstract = "Ethane dimethanesulphonate (EDS), an alkylating agent, caused marked atrophy of the adrenal cortex of adult male rats, in addition to its toxic effect on testicular Leydig cells. The aim of this work was to examine whether a 9-day treatment with ACTH (40 IU/kg/d), isoproterenol (120 mg/kg/d) or dexamethasone (0.25 mg/kg/d), which started 4 days prior to intraperitoneal administration of a single dose of EDS (75 mg/kg), affected the morphological changes in the adrenal cortex evoked by EDS alone. The animals were killed 15 days after EDS injection. Stereological analysis revealed that both ACTH and isoproterenol almost completely prevented cortical atrophy induced by EDS. They also considerably stimulated corticosterone secretion in EDS-injected animals. By contrast, in dexamethasone-suppressed rats, the deleterious effect of EDS on adrenocortical cells was augmented. The volume and cellularity of all cortical zones were reduced, but the remaining cells of the zona reticularis displayed considerable hypertrophy which was probably responsible for the maintenance of corticosterone secretion. These results clearly demonstrate that both ACTH and b adrenoceptor stimulation have protective action against the toxic effects of EDS on rat adrenal cortex, whereas dexamethasone exerts an opposite influence., Alkilirajući agens etandimetansulfonat (EDS), pored toksičnog delovanja na Lajdigove ćelije semenika, izaziva izraženu atrofiju kore nadbubrežne žlezde odraslih pacova. U ovom radu je ispitivan uticaj devetodnevnog tretmana sa ACTH (40 IU/kg/d), izoproterenolom (120 mg/kg/d) ili deksametazonom (0.25 mg/kg/d) na morfološke promene kore nadbubrega koje izaziva jedna intraperitonealna injekcija EDS (75 mg/kg). Tretmani su započeli 4 dana pre davanja EDS i nastavljeni su još 5 dana, a životinje su žrtvovane 15 dana posle aplikacije EDS. U prisustvu ACTH ili izoproterenola ne ispoljavaju se promene u stereološkim parametrima kore nadbubrega koje izaziva EDS. Oba tretmana značajno povećavaju i koncentraciju kortikosterona u serumu. Deksametazon, međutim, pojačava toksične efekte EDS; zapremina svih zona kore i broj parenhimskih ćelija u zonama su smanjeni, ali preostale ćelije retikularne zone pokazuju značajnu hipertrofiju, koja je verovatno odgovorna za održavanje sekrecije kortikosterona kod ovih životinja. Rezultati rada pokazuju da se toksični efekti EDS na koru nadbubrega mogu sprečiti visokim nivoom ACTH ili stimulacijom b adrenalinskih receptora, a potencirati egzogenim glukokortikoidom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "The effects of acth, isoproterenol and Dexamethasone on the rat adrenal gland response to ethane dimethanesulphonate (Eds): A stereological study, Uticaj ACTH, izoproterenola i deksametazona na odgovor nadbubrežne žlezde pacova na etandimetansulfonat (EDS): stereološko ispitivanje",
volume = "53",
number = "5-6",
pages = "333-342",
doi = "10.2298/AVB0306333P"
}

Plećaš-Solarović, B., Pešić, V., Mirković, D.,& Leposavić, G.. (2003). The effects of acth, isoproterenol and Dexamethasone on the rat adrenal gland response to ethane dimethanesulphonate (Eds): A stereological study. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 53(5-6), 333-342.
https://doi.org/10.2298/AVB0306333P

Plećaš-Solarović B, Pešić V, Mirković D, Leposavić G. The effects of acth, isoproterenol and Dexamethasone on the rat adrenal gland response to ethane dimethanesulphonate (Eds): A stereological study. in Acta veterinaria. 2003;53(5-6):333-342.
doi:10.2298/AVB0306333P .

Plećaš-Solarović, Bosiljka, Pešić, Vesna, Mirković, Duško, Leposavić, Gordana, "The effects of acth, isoproterenol and Dexamethasone on the rat adrenal gland response to ethane dimethanesulphonate (Eds): A stereological study" in Acta veterinaria, 53, no. 5-6 (2003):333-342,
https://doi.org/10.2298/AVB0306333P . .

TY  - JOUR
AU  - Rauski, Aleksandra
AU  - Kosec, Duško
AU  - Vidić-Danković, Biljana
AU  - Plećaš-Solarović, Bosiljka
AU  - Leposavić, Gordana
PY  - 2003
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/466
AB  - The study revealed that β-adrenoceptor blockade with propranolol (0. 40 mg/l00 g/day, s.c.) in adult male DA rats: (i) increased the thymocyte proliferation and apoptosis, (ii) caused disturbances in kinetics of T cell differentiation leading to distinguishable changes in relative proportion of thymocytes at distinct maturational steps and to an expansion of the most mature single positive (CD4+, CD8+) thymocyte pool, (iii) affected the relative proportion of neither CD4+ nor CD8+ peripheral blood lymphocytes (PBL), and (iv) augmented the relative number of CD8+CD25+ cells. Thus, the results suggest the role of β-adrenoceptors) in fine-tuning of T cell maturation, and, possibly, distribution and activation of distinct PBL subsets.
PB  - Taylor and Francis Ltd.
T2  - International Journal of Neuroscience
T1  - Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes
VL  - 113
IS  - 12
SP  - 1653
EP  - 1673
DO  - 10.1080/00207450390245216
ER  - 

@article{
author = "Rauski, Aleksandra and Kosec, Duško and Vidić-Danković, Biljana and Plećaš-Solarović, Bosiljka and Leposavić, Gordana",
year = "2003",
abstract = "The study revealed that β-adrenoceptor blockade with propranolol (0. 40 mg/l00 g/day, s.c.) in adult male DA rats: (i) increased the thymocyte proliferation and apoptosis, (ii) caused disturbances in kinetics of T cell differentiation leading to distinguishable changes in relative proportion of thymocytes at distinct maturational steps and to an expansion of the most mature single positive (CD4+, CD8+) thymocyte pool, (iii) affected the relative proportion of neither CD4+ nor CD8+ peripheral blood lymphocytes (PBL), and (iv) augmented the relative number of CD8+CD25+ cells. Thus, the results suggest the role of β-adrenoceptors) in fine-tuning of T cell maturation, and, possibly, distribution and activation of distinct PBL subsets.",
publisher = "Taylor and Francis Ltd.",
journal = "International Journal of Neuroscience",
title = "Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes",
volume = "113",
number = "12",
pages = "1653-1673",
doi = "10.1080/00207450390245216"
}

Rauski, A., Kosec, D., Vidić-Danković, B., Plećaš-Solarović, B.,& Leposavić, G.. (2003). Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes. in International Journal of Neuroscience
Taylor and Francis Ltd.., 113(12), 1653-1673.
https://doi.org/10.1080/00207450390245216

Rauski A, Kosec D, Vidić-Danković B, Plećaš-Solarović B, Leposavić G. Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes. in International Journal of Neuroscience. 2003;113(12):1653-1673.
doi:10.1080/00207450390245216 .

Rauski, Aleksandra, Kosec, Duško, Vidić-Danković, Biljana, Plećaš-Solarović, Bosiljka, Leposavić, Gordana, "Effects of beta-adrenoceptor blockade on the phenotypic characteristics of thymocytes and peripheral blood lymphocytes" in International Journal of Neuroscience, 113, no. 12 (2003):1653-1673,
https://doi.org/10.1080/00207450390245216 . .