TY  - JOUR
AU  - Lukić, Vera
AU  - Micić, Ružica
AU  - Arsić, Biljana
AU  - Mitić, Milan
AU  - Jovanović, Miloš
AU  - Pavlović, Aleksandra
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4050
AB  - The samples of plant material suspected to contain new psychoactive substances are very often the subject of chemical-toxicological analyses. Gas chromatography-mass spectrometry (MS), liquid chromatography-quadrupole time-of-flight-MS, and liquid chromatography-tandem MS were applied with the aim to identify synthetic cannabinoid, methyl 2-{[1-(cyclohexylmethyl)-1H-indol-3-yl] formamido}-3-methylbutanoate (MMB-CHMICA) without the analytical standard, which is very often the case when a new drug arrives. The structure of compound was also confirmed by one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy and conformational analysis. After identification, methanolic extract of plant material containing MMB-CHMICA was successfully used for developing a multiple reaction monitoring method on liquid chromatography-tandem MS instrument. The optimization procedure is shown in detail. The complete fragmentation pattern and also the optimization of the extraction procedure of MMB-CHMICA from plant material were shown. The obtained data are useful for forensic, toxicological, and clinical purposes.
PB  - De Gruyter Open Ltd
T2  - Open Chemistry
T1  - Identification of synthetic cannabinoid methyl 2-{[1-(cyclohexylmethyl)-1H-indol-3-yl] formamido}-3-methylbutanoate using modern mass spectrometry and nuclear magnetic resonance techniques
VL  - 19
IS  - 1
SP  - 1259
EP  - 1273
DO  - 10.1515/chem-2021-0113
ER  - 

@article{
author = "Lukić, Vera and Micić, Ružica and Arsić, Biljana and Mitić, Milan and Jovanović, Miloš and Pavlović, Aleksandra",
year = "2021",
abstract = "The samples of plant material suspected to contain new psychoactive substances are very often the subject of chemical-toxicological analyses. Gas chromatography-mass spectrometry (MS), liquid chromatography-quadrupole time-of-flight-MS, and liquid chromatography-tandem MS were applied with the aim to identify synthetic cannabinoid, methyl 2-{[1-(cyclohexylmethyl)-1H-indol-3-yl] formamido}-3-methylbutanoate (MMB-CHMICA) without the analytical standard, which is very often the case when a new drug arrives. The structure of compound was also confirmed by one-dimensional and two-dimensional nuclear magnetic resonance spectroscopy and conformational analysis. After identification, methanolic extract of plant material containing MMB-CHMICA was successfully used for developing a multiple reaction monitoring method on liquid chromatography-tandem MS instrument. The optimization procedure is shown in detail. The complete fragmentation pattern and also the optimization of the extraction procedure of MMB-CHMICA from plant material were shown. The obtained data are useful for forensic, toxicological, and clinical purposes.",
publisher = "De Gruyter Open Ltd",
journal = "Open Chemistry",
title = "Identification of synthetic cannabinoid methyl 2-{[1-(cyclohexylmethyl)-1H-indol-3-yl] formamido}-3-methylbutanoate using modern mass spectrometry and nuclear magnetic resonance techniques",
volume = "19",
number = "1",
pages = "1259-1273",
doi = "10.1515/chem-2021-0113"
}

Lukić, V., Micić, R., Arsić, B., Mitić, M., Jovanović, M.,& Pavlović, A.. (2021). Identification of synthetic cannabinoid methyl 2-{[1-(cyclohexylmethyl)-1H-indol-3-yl] formamido}-3-methylbutanoate using modern mass spectrometry and nuclear magnetic resonance techniques. in Open Chemistry
De Gruyter Open Ltd., 19(1), 1259-1273.
https://doi.org/10.1515/chem-2021-0113

Lukić V, Micić R, Arsić B, Mitić M, Jovanović M, Pavlović A. Identification of synthetic cannabinoid methyl 2-{[1-(cyclohexylmethyl)-1H-indol-3-yl] formamido}-3-methylbutanoate using modern mass spectrometry and nuclear magnetic resonance techniques. in Open Chemistry. 2021;19(1):1259-1273.
doi:10.1515/chem-2021-0113 .

Lukić, Vera, Micić, Ružica, Arsić, Biljana, Mitić, Milan, Jovanović, Miloš, Pavlović, Aleksandra, "Identification of synthetic cannabinoid methyl 2-{[1-(cyclohexylmethyl)-1H-indol-3-yl] formamido}-3-methylbutanoate using modern mass spectrometry and nuclear magnetic resonance techniques" in Open Chemistry, 19, no. 1 (2021):1259-1273,
https://doi.org/10.1515/chem-2021-0113 . .

TY  - JOUR
AU  - Novaković, Ivana
AU  - Maksimović, Nela
AU  - Pavlović, Aleksandra
AU  - Žarković, Milena
AU  - Rovcanin, Branislav
AU  - Mirković, Duško
AU  - Pekmezović, Tatjana
AU  - Cvetković, Dragana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2148
AB  - Molecular genetic testing is part of modern medical practice. DNA tests are an essential part of diagnostics and genetic counseling in single gene diseases, while their application in polygenic disorders is still limited. Pharmacogenetics studies DNA variants associated with variations in drug efficacy and toxicity, and tests in this field are being developed rapidly. The main method for molecular genetic testing is the polymerase chain reaction, with a number of modifications. New methods, such as next generation sequencing and DNA microarray, should allow simultaneous analysis of a number of genes, even whole genome sequencing. Ethical concerns in molecular genetic testing are very important, along with legislation. After molecular genetic testing, interpretation of results and genetic counseling should be done by professionals. With the example of thrombophilia, we discuss questions about genetic testing, its possibilities and promises.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Introduction to Molecular genetic diagnostics
VL  - 33
IS  - 1
SP  - 3
EP  - 7
DO  - 10.2478/jomb-2013-0039
ER  - 

@article{
author = "Novaković, Ivana and Maksimović, Nela and Pavlović, Aleksandra and Žarković, Milena and Rovcanin, Branislav and Mirković, Duško and Pekmezović, Tatjana and Cvetković, Dragana",
year = "2014",
abstract = "Molecular genetic testing is part of modern medical practice. DNA tests are an essential part of diagnostics and genetic counseling in single gene diseases, while their application in polygenic disorders is still limited. Pharmacogenetics studies DNA variants associated with variations in drug efficacy and toxicity, and tests in this field are being developed rapidly. The main method for molecular genetic testing is the polymerase chain reaction, with a number of modifications. New methods, such as next generation sequencing and DNA microarray, should allow simultaneous analysis of a number of genes, even whole genome sequencing. Ethical concerns in molecular genetic testing are very important, along with legislation. After molecular genetic testing, interpretation of results and genetic counseling should be done by professionals. With the example of thrombophilia, we discuss questions about genetic testing, its possibilities and promises.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Introduction to Molecular genetic diagnostics",
volume = "33",
number = "1",
pages = "3-7",
doi = "10.2478/jomb-2013-0039"
}

Novaković, I., Maksimović, N., Pavlović, A., Žarković, M., Rovcanin, B., Mirković, D., Pekmezović, T.,& Cvetković, D.. (2014). Introduction to Molecular genetic diagnostics. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 33(1), 3-7.
https://doi.org/10.2478/jomb-2013-0039

Novaković I, Maksimović N, Pavlović A, Žarković M, Rovcanin B, Mirković D, Pekmezović T, Cvetković D. Introduction to Molecular genetic diagnostics. in Journal of Medical Biochemistry. 2014;33(1):3-7.
doi:10.2478/jomb-2013-0039 .

Novaković, Ivana, Maksimović, Nela, Pavlović, Aleksandra, Žarković, Milena, Rovcanin, Branislav, Mirković, Duško, Pekmezović, Tatjana, Cvetković, Dragana, "Introduction to Molecular genetic diagnostics" in Journal of Medical Biochemistry, 33, no. 1 (2014):3-7,
https://doi.org/10.2478/jomb-2013-0039 . .