TY  - JOUR
AU  - Milinković Budinčić, Jelena
AU  - Petrović, Lidija
AU  - Đekić, Ljiljana
AU  - Aleksić, Milijana
AU  - Fraj, Jadranka
AU  - Popović, Senka
AU  - Bučko, Sandra
AU  - Katona, Jaroslav
AU  - Spasojević, Ljiljana
AU  - Škrbić, Jelena
AU  - Malenović, Anđelija
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4021
AB  - Microencapsulation of bioactive substances is a common strategy for their protection and release rate control. The use of chitosan (Ch) is particularly promising due to its abundance, biocompatibility, and interaction with anionic surfactants to form complexes of different characteristics with relevance for use in microcapsule wall design. In this study, Ch/sodium dodecyl sulfate (SDS) microcapsules, without and with cross-linking agent (formaldehyde (FA) or glutaraldehyde (GA)), were obtained by the spray drying of vitamin E loaded oil-in-water emulsion. All of the microcapsules had good stability during the drying process. Depending on the composition, their product yield, moisture content, and encapsulation efficiency varied between 11–34%, 1.14–1.62%, and 94–126%, respectively. SEM and FTIR analysis results indicate that SDS as well as cross-linkers significantly affected the microcapsule wall properties. The profiles of in vitro vitamin E release from the investigated microcapsules fit with the Korsmeyer-Peppas model (r2 > 0.9). The chemical structure of the anionic surfactant was found to have a significant effect on the vitamin E release mechanism. Ch/SDS coacervates may build a microcapsule wall without toxic crosslinkers. This enabled the combined diffusion/swelling based release mechanism of the encapsulated lipophilic substance, which can be considered favorable for utilization in food and pharmaceutical products.
PB  - MDPI
T2  - Pharmaceuticals
T1  - Chitosan/Sodium Dodecyl Sulfate Complexes for Microencapsulation of Vitamin E and Its Release Profile— Understanding the Effect of Anionic Surfactant
VL  - 15
IS  - 1
DO  - 10.3390/ph15010054
ER  - 

@article{
author = "Milinković Budinčić, Jelena and Petrović, Lidija and Đekić, Ljiljana and Aleksić, Milijana and Fraj, Jadranka and Popović, Senka and Bučko, Sandra and Katona, Jaroslav and Spasojević, Ljiljana and Škrbić, Jelena and Malenović, Anđelija",
year = "2021",
abstract = "Microencapsulation of bioactive substances is a common strategy for their protection and release rate control. The use of chitosan (Ch) is particularly promising due to its abundance, biocompatibility, and interaction with anionic surfactants to form complexes of different characteristics with relevance for use in microcapsule wall design. In this study, Ch/sodium dodecyl sulfate (SDS) microcapsules, without and with cross-linking agent (formaldehyde (FA) or glutaraldehyde (GA)), were obtained by the spray drying of vitamin E loaded oil-in-water emulsion. All of the microcapsules had good stability during the drying process. Depending on the composition, their product yield, moisture content, and encapsulation efficiency varied between 11–34%, 1.14–1.62%, and 94–126%, respectively. SEM and FTIR analysis results indicate that SDS as well as cross-linkers significantly affected the microcapsule wall properties. The profiles of in vitro vitamin E release from the investigated microcapsules fit with the Korsmeyer-Peppas model (r2 > 0.9). The chemical structure of the anionic surfactant was found to have a significant effect on the vitamin E release mechanism. Ch/SDS coacervates may build a microcapsule wall without toxic crosslinkers. This enabled the combined diffusion/swelling based release mechanism of the encapsulated lipophilic substance, which can be considered favorable for utilization in food and pharmaceutical products.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "Chitosan/Sodium Dodecyl Sulfate Complexes for Microencapsulation of Vitamin E and Its Release Profile— Understanding the Effect of Anionic Surfactant",
volume = "15",
number = "1",
doi = "10.3390/ph15010054"
}

Milinković Budinčić, J., Petrović, L., Đekić, L., Aleksić, M., Fraj, J., Popović, S., Bučko, S., Katona, J., Spasojević, L., Škrbić, J.,& Malenović, A.. (2021). Chitosan/Sodium Dodecyl Sulfate Complexes for Microencapsulation of Vitamin E and Its Release Profile— Understanding the Effect of Anionic Surfactant. in Pharmaceuticals
MDPI., 15(1).
https://doi.org/10.3390/ph15010054

Milinković Budinčić J, Petrović L, Đekić L, Aleksić M, Fraj J, Popović S, Bučko S, Katona J, Spasojević L, Škrbić J, Malenović A. Chitosan/Sodium Dodecyl Sulfate Complexes for Microencapsulation of Vitamin E and Its Release Profile— Understanding the Effect of Anionic Surfactant. in Pharmaceuticals. 2021;15(1).
doi:10.3390/ph15010054 .

Milinković Budinčić, Jelena, Petrović, Lidija, Đekić, Ljiljana, Aleksić, Milijana, Fraj, Jadranka, Popović, Senka, Bučko, Sandra, Katona, Jaroslav, Spasojević, Ljiljana, Škrbić, Jelena, Malenović, Anđelija, "Chitosan/Sodium Dodecyl Sulfate Complexes for Microencapsulation of Vitamin E and Its Release Profile— Understanding the Effect of Anionic Surfactant" in Pharmaceuticals, 15, no. 1 (2021),
https://doi.org/10.3390/ph15010054 . .

TY  - JOUR
AU  - Milinković Budinčić, Jelena
AU  - Petrović, Lidija
AU  - Đekić, Ljiljana
AU  - Fraj, Jadranka
AU  - Bučko, Sandra
AU  - Katona, Jaroslav
AU  - Spasojević, Ljiljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3774
AB  - Potential benefit of microencapsulation is its ability to deliver and protect incorporated ingredients such as vitamin E. Microcapsule wall properties can be changed by adding of coss-linking agents that are usually considered toxic for application. The microcapsules were prepared by a spray-drying technique using coacervation method, by depositing the coacervate formed in the mixture of chitosan and sodium lauryl ether sulfate to the oil/water interface. All obtained microcapsules suspensions had slightly lower mean diameter compared to the starting emulsion (6.85 ± 0.213 μm), which shows their good stability during the drying process. The choice and absence of cross-linking agents had influence on kinetics of vitamin E release. Encapsulation efficiency of microcapsules without cross-linking agent was 73.17 ± 0.64 %. This study avoided the use of aldehydes as cross-linking agents and found that chitosan/SLES complex can be used as wall material for the microencapsulation of hydrophobic active molecules in cosmetic industry.
PB  - Elsevier Ltd
T2  - Carbohydrate Polymers
T1  - Study of vitamin E microencapsulation and controlled release from chitosan/sodium lauryl ether sulfate microcapsules
VL  - 251
DO  - 10.1016/j.carbpol.2020.116988
ER  - 

@article{
author = "Milinković Budinčić, Jelena and Petrović, Lidija and Đekić, Ljiljana and Fraj, Jadranka and Bučko, Sandra and Katona, Jaroslav and Spasojević, Ljiljana",
year = "2021",
abstract = "Potential benefit of microencapsulation is its ability to deliver and protect incorporated ingredients such as vitamin E. Microcapsule wall properties can be changed by adding of coss-linking agents that are usually considered toxic for application. The microcapsules were prepared by a spray-drying technique using coacervation method, by depositing the coacervate formed in the mixture of chitosan and sodium lauryl ether sulfate to the oil/water interface. All obtained microcapsules suspensions had slightly lower mean diameter compared to the starting emulsion (6.85 ± 0.213 μm), which shows their good stability during the drying process. The choice and absence of cross-linking agents had influence on kinetics of vitamin E release. Encapsulation efficiency of microcapsules without cross-linking agent was 73.17 ± 0.64 %. This study avoided the use of aldehydes as cross-linking agents and found that chitosan/SLES complex can be used as wall material for the microencapsulation of hydrophobic active molecules in cosmetic industry.",
publisher = "Elsevier Ltd",
journal = "Carbohydrate Polymers",
title = "Study of vitamin E microencapsulation and controlled release from chitosan/sodium lauryl ether sulfate microcapsules",
volume = "251",
doi = "10.1016/j.carbpol.2020.116988"
}

Milinković Budinčić, J., Petrović, L., Đekić, L., Fraj, J., Bučko, S., Katona, J.,& Spasojević, L.. (2021). Study of vitamin E microencapsulation and controlled release from chitosan/sodium lauryl ether sulfate microcapsules. in Carbohydrate Polymers
Elsevier Ltd., 251.
https://doi.org/10.1016/j.carbpol.2020.116988

Milinković Budinčić J, Petrović L, Đekić L, Fraj J, Bučko S, Katona J, Spasojević L. Study of vitamin E microencapsulation and controlled release from chitosan/sodium lauryl ether sulfate microcapsules. in Carbohydrate Polymers. 2021;251.
doi:10.1016/j.carbpol.2020.116988 .

Milinković Budinčić, Jelena, Petrović, Lidija, Đekić, Ljiljana, Fraj, Jadranka, Bučko, Sandra, Katona, Jaroslav, Spasojević, Ljiljana, "Study of vitamin E microencapsulation and controlled release from chitosan/sodium lauryl ether sulfate microcapsules" in Carbohydrate Polymers, 251 (2021),
https://doi.org/10.1016/j.carbpol.2020.116988 . .

TY  - JOUR
AU  - Fraj, Jadranka
AU  - Petrović, Lidija
AU  - Đekić, Ljiljana
AU  - Milinković Budinčić, Jelena
AU  - Bučko, Sandra
AU  - Katona, Jaroslav
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3695
AB  - The objective of this study was to produce and characterize microcapsules for simultaneous encapsulation hydrophilic and lipophilic active substances. For this purpose, double emulsification process was employed, followed by complex coacervation in the system of two oppositely charged biopolymers, gelatin and sodium caseinate (NaCAS). Properties of the microcapsules wall have been regulated by cross-linking of the gelatin/NaCAS complex at the interface with genipin. Vitamins C and E were selected as model hydrophilic and lipophilic bioactive compounds for this study. Investigations of surface morphology, encapsulation efficiency (EE) and kinetic of vitamin C release have shown that genipin concentration as well as interaction in gelatin/NaCAS system make an influence on microcapsules properties. Genipin concentration of 2 mmol/g, was chosen as the optimal and the highest EE of the vitamins were obtained at proteins mass ratio of 2:1. The results of release kinetic determination of the vitamins showed that release mechanism is simple diffusion.
PB  - Elsevier Ltd
T2  - Journal of Food Engineering
T1  - Encapsulation and release of vitamin C in double W/O/W emulsions followed by complex coacervation in gelatin-sodium caseinate system
VL  - 292
DO  - 10.1016/j.jfoodeng.2020.110353
ER  - 

@article{
author = "Fraj, Jadranka and Petrović, Lidija and Đekić, Ljiljana and Milinković Budinčić, Jelena and Bučko, Sandra and Katona, Jaroslav",
year = "2021",
abstract = "The objective of this study was to produce and characterize microcapsules for simultaneous encapsulation hydrophilic and lipophilic active substances. For this purpose, double emulsification process was employed, followed by complex coacervation in the system of two oppositely charged biopolymers, gelatin and sodium caseinate (NaCAS). Properties of the microcapsules wall have been regulated by cross-linking of the gelatin/NaCAS complex at the interface with genipin. Vitamins C and E were selected as model hydrophilic and lipophilic bioactive compounds for this study. Investigations of surface morphology, encapsulation efficiency (EE) and kinetic of vitamin C release have shown that genipin concentration as well as interaction in gelatin/NaCAS system make an influence on microcapsules properties. Genipin concentration of 2 mmol/g, was chosen as the optimal and the highest EE of the vitamins were obtained at proteins mass ratio of 2:1. The results of release kinetic determination of the vitamins showed that release mechanism is simple diffusion.",
publisher = "Elsevier Ltd",
journal = "Journal of Food Engineering",
title = "Encapsulation and release of vitamin C in double W/O/W emulsions followed by complex coacervation in gelatin-sodium caseinate system",
volume = "292",
doi = "10.1016/j.jfoodeng.2020.110353"
}

Fraj, J., Petrović, L., Đekić, L., Milinković Budinčić, J., Bučko, S.,& Katona, J.. (2021). Encapsulation and release of vitamin C in double W/O/W emulsions followed by complex coacervation in gelatin-sodium caseinate system. in Journal of Food Engineering
Elsevier Ltd., 292.
https://doi.org/10.1016/j.jfoodeng.2020.110353

Fraj J, Petrović L, Đekić L, Milinković Budinčić J, Bučko S, Katona J. Encapsulation and release of vitamin C in double W/O/W emulsions followed by complex coacervation in gelatin-sodium caseinate system. in Journal of Food Engineering. 2021;292.
doi:10.1016/j.jfoodeng.2020.110353 .

Fraj, Jadranka, Petrović, Lidija, Đekić, Ljiljana, Milinković Budinčić, Jelena, Bučko, Sandra, Katona, Jaroslav, "Encapsulation and release of vitamin C in double W/O/W emulsions followed by complex coacervation in gelatin-sodium caseinate system" in Journal of Food Engineering, 292 (2021),
https://doi.org/10.1016/j.jfoodeng.2020.110353 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Martinović, Martina
AU  - Ćirić, Ana
AU  - Fraj, Jadranka
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5372
AB  - The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25–1% of the polymer and 5% of the drug. The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21–22.56 nm) embedded within pseudoplastic chitosan hydrogel. In vitro ibuprofen release was sustained for 12 h and followed zero-order kinetics. pH values of the composite hydrogels were in the range of 4.80–5.27, thus physiologically acceptable. The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh−1cm−2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (−12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal. This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient’s compliance, were performed.
PB  - Taylor & Francis
T2  - Pharmaceutical Development and Technology
T1  - Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics
VL  - 25
IS  - 3
SP  - 332
EP  - 339
DO  - 10.1080/10837450.2019.1701495
ER  - 

@article{
author = "Đekić, Ljiljana and Martinović, Martina and Ćirić, Ana and Fraj, Jadranka",
year = "2020",
abstract = "The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25–1% of the polymer and 5% of the drug. The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21–22.56 nm) embedded within pseudoplastic chitosan hydrogel. In vitro ibuprofen release was sustained for 12 h and followed zero-order kinetics. pH values of the composite hydrogels were in the range of 4.80–5.27, thus physiologically acceptable. The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh−1cm−2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (−12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal. This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient’s compliance, were performed.",
publisher = "Taylor & Francis",
journal = "Pharmaceutical Development and Technology",
title = "Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics",
volume = "25",
number = "3",
pages = "332-339",
doi = "10.1080/10837450.2019.1701495"
}

Đekić, L., Martinović, M., Ćirić, A.,& Fraj, J.. (2020). Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics. in Pharmaceutical Development and Technology
Taylor & Francis., 25(3), 332-339.
https://doi.org/10.1080/10837450.2019.1701495

Đekić L, Martinović M, Ćirić A, Fraj J. Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics. in Pharmaceutical Development and Technology. 2020;25(3):332-339.
doi:10.1080/10837450.2019.1701495 .

Đekić, Ljiljana, Martinović, Martina, Ćirić, Ana, Fraj, Jadranka, "Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics" in Pharmaceutical Development and Technology, 25, no. 3 (2020):332-339,
https://doi.org/10.1080/10837450.2019.1701495 . .

TY  - CONF
AU  - Milinković Budinčić, Jelena
AU  - Đekić, Ljiljana
AU  - Petrović, Lidija
AU  - Fraj, Jadranka
AU  - Ćirić, Ana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5369
AB  - INTRODUCTION: The important current focus
in production of cosmetics is usage of vitamin E
(E), a natural antioxidant protective for tissues
from UV radiation, delays photoaging and provide
moisturizing effect. Encapsulation is needed
for its protection from high temperature, oxygen,
and light, during storage, and also for a potential
ability to control its release and delivery. Preparation
of microcapsules of desired characteristics
depends on various factors (size and nature of the
core substance, wall material, techniques and parameters
of encapsulation) [1, 2]. The study aimed
to evaluate chitosan/sodium lauryl ether sulfate
(Ch/SLES) microcapsules with E as a delivery system
for skin care.
MATERIALS AND METHODS: Microcapsules
were prepared by complex coacervation. Initially,
a 20% O/W emulsion with E (10% solution
in medium-chain triglycerides), stabilized with
the mixture of Ch (0.1 %) and SLES [3], was obtained
by Ultra Turrax T25 homogenization. The
emulsion, without or with a crosslinker, formaldehyde
(FA) or glutaraldehyde (GA), was spray
dried. The in vitro release profile of E from the microcapsule
samples (0.1 g) was studied in 100 g of
ethanol 80%, under continuous stirring at room
temperature. The dissolved E in supernatant aliquots
(2 ml) was analyzed during 90 min, by the
Halo DB-20S UV-VIS spectrophotometer.
RESULTS: The obtained release profiles were
analyzed by fi tt ing in different mathematical
models and in all samples correlate the best with
Korsmeyer-Peppas model. The diffusion exponent
n values (0.05-0.23) indicated non-Fickian
diffusion. We assumed that release of E was
based on a combination of rinsing from the surface
of the microcapsules [4] and diffusion
through the capsule wall. For microparticles with
GA, n was the lowest, the release was rapid and
the amount of release of the substance was higher
(i.e., more pronounced rinsing process), compared
with FA and microcapsules without the
crosslinker, where release of E was more controlled
by diffusion.
CONCLUSION: E vitamin release from Ch/
SLES microcapsules followed Korsmeyer-Peppas
kinetics. The selection of the crosslinker influenced
their surface properties, the surface amount and permeability of the capsule wall for E vitamine
diffusion.
PB  - Hungarian Society for Pharmaceutical Sciences
C3  - Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts
T1  - Chitosan/sodium lauryl ether sulfate microcapsules as carriers for vitamin E: in vitro release study
VL  - 88
IS  - 043
SP  - 173
EP  - 174
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5369
ER  - 

@conference{
author = "Milinković Budinčić, Jelena and Đekić, Ljiljana and Petrović, Lidija and Fraj, Jadranka and Ćirić, Ana",
year = "2018",
abstract = "INTRODUCTION: The important current focus
in production of cosmetics is usage of vitamin E
(E), a natural antioxidant protective for tissues
from UV radiation, delays photoaging and provide
moisturizing effect. Encapsulation is needed
for its protection from high temperature, oxygen,
and light, during storage, and also for a potential
ability to control its release and delivery. Preparation
of microcapsules of desired characteristics
depends on various factors (size and nature of the
core substance, wall material, techniques and parameters
of encapsulation) [1, 2]. The study aimed
to evaluate chitosan/sodium lauryl ether sulfate
(Ch/SLES) microcapsules with E as a delivery system
for skin care.
MATERIALS AND METHODS: Microcapsules
were prepared by complex coacervation. Initially,
a 20% O/W emulsion with E (10% solution
in medium-chain triglycerides), stabilized with
the mixture of Ch (0.1 %) and SLES [3], was obtained
by Ultra Turrax T25 homogenization. The
emulsion, without or with a crosslinker, formaldehyde
(FA) or glutaraldehyde (GA), was spray
dried. The in vitro release profile of E from the microcapsule
samples (0.1 g) was studied in 100 g of
ethanol 80%, under continuous stirring at room
temperature. The dissolved E in supernatant aliquots
(2 ml) was analyzed during 90 min, by the
Halo DB-20S UV-VIS spectrophotometer.
RESULTS: The obtained release profiles were
analyzed by fi tt ing in different mathematical
models and in all samples correlate the best with
Korsmeyer-Peppas model. The diffusion exponent
n values (0.05-0.23) indicated non-Fickian
diffusion. We assumed that release of E was
based on a combination of rinsing from the surface
of the microcapsules [4] and diffusion
through the capsule wall. For microparticles with
GA, n was the lowest, the release was rapid and
the amount of release of the substance was higher
(i.e., more pronounced rinsing process), compared
with FA and microcapsules without the
crosslinker, where release of E was more controlled
by diffusion.
CONCLUSION: E vitamin release from Ch/
SLES microcapsules followed Korsmeyer-Peppas
kinetics. The selection of the crosslinker influenced
their surface properties, the surface amount and permeability of the capsule wall for E vitamine
diffusion.",
publisher = "Hungarian Society for Pharmaceutical Sciences",
journal = "Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts",
title = "Chitosan/sodium lauryl ether sulfate microcapsules as carriers for vitamin E: in vitro release study",
volume = "88",
number = "043",
pages = "173-174",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5369"
}

Milinković Budinčić, J., Đekić, L., Petrović, L., Fraj, J.,& Ćirić, A.. (2018). Chitosan/sodium lauryl ether sulfate microcapsules as carriers for vitamin E: in vitro release study. in Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts
Hungarian Society for Pharmaceutical Sciences., 88(043), 173-174.
https://hdl.handle.net/21.15107/rcub_farfar_5369

Milinković Budinčić J, Đekić L, Petrović L, Fraj J, Ćirić A. Chitosan/sodium lauryl ether sulfate microcapsules as carriers for vitamin E: in vitro release study. in Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts. 2018;88(043):173-174.
https://hdl.handle.net/21.15107/rcub_farfar_5369 .

Milinković Budinčić, Jelena, Đekić, Ljiljana, Petrović, Lidija, Fraj, Jadranka, Ćirić, Ana, "Chitosan/sodium lauryl ether sulfate microcapsules as carriers for vitamin E: in vitro release study" in Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts, 88, no. 043 (2018):173-174,
https://hdl.handle.net/21.15107/rcub_farfar_5369 .