TY  - JOUR
AU  - Pavlović, Dragana R.
AU  - Lakušić, Branislava
AU  - Došlov-Kokoruš, Zvezdana
AU  - Kovačević, Nada
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1158
AB  - Quantitative analyses and investigation of antioxidant activity of herb and dry ethanolic extracts of five species from Ericaceae family (Arbutus unedo L., Bruckentalia spiculifolia Rchb., Calluna vulgaris Salisb., Erica arborea L. and Erica carnea L.) were performed. Total polyphenols, tannins and flavonoids were determined spectrophotometrically and arbutin content was measured both spectrophotometrically and by HPLC coupled with DAD detection Antioxidative properites of the ethanolic extracts were tested by means of FRAP (total antioxidant capacity), lipid peroxidation and DPPH free radical scavenging activity. A significant amount of arbutin was detected only in Arbutus unedo. All samples investigated showed excellent antioxidant activity. The best inhibition of lipid peroxidation has been shown by Bruckentalia spiculifolia herb extract (62.5 mu g/ml; more than 95%), which contained the highest amount of flavonoids (11.79%). The highest scavenging activity was obtained with leave extract of Arbutus unedd (IC(50) = 7.14 mu g/ml). The leaves of A. unedd contained a small amount of flavonoids but high content of non-tannins polyphenols.
PB  - Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn
T2  - Pharmazie
T1  - Arbutin content and antioxidant activity of some Ericaceae species
VL  - 64
IS  - 10
SP  - 656
EP  - 659
DO  - 10.1691/ph.2009.9551
ER  - 

@article{
author = "Pavlović, Dragana R. and Lakušić, Branislava and Došlov-Kokoruš, Zvezdana and Kovačević, Nada",
year = "2009",
abstract = "Quantitative analyses and investigation of antioxidant activity of herb and dry ethanolic extracts of five species from Ericaceae family (Arbutus unedo L., Bruckentalia spiculifolia Rchb., Calluna vulgaris Salisb., Erica arborea L. and Erica carnea L.) were performed. Total polyphenols, tannins and flavonoids were determined spectrophotometrically and arbutin content was measured both spectrophotometrically and by HPLC coupled with DAD detection Antioxidative properites of the ethanolic extracts were tested by means of FRAP (total antioxidant capacity), lipid peroxidation and DPPH free radical scavenging activity. A significant amount of arbutin was detected only in Arbutus unedo. All samples investigated showed excellent antioxidant activity. The best inhibition of lipid peroxidation has been shown by Bruckentalia spiculifolia herb extract (62.5 mu g/ml; more than 95%), which contained the highest amount of flavonoids (11.79%). The highest scavenging activity was obtained with leave extract of Arbutus unedd (IC(50) = 7.14 mu g/ml). The leaves of A. unedd contained a small amount of flavonoids but high content of non-tannins polyphenols.",
publisher = "Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn",
journal = "Pharmazie",
title = "Arbutin content and antioxidant activity of some Ericaceae species",
volume = "64",
number = "10",
pages = "656-659",
doi = "10.1691/ph.2009.9551"
}

Pavlović, D. R., Lakušić, B., Došlov-Kokoruš, Z.,& Kovačević, N.. (2009). Arbutin content and antioxidant activity of some Ericaceae species. in Pharmazie
Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn., 64(10), 656-659.
https://doi.org/10.1691/ph.2009.9551

Pavlović DR, Lakušić B, Došlov-Kokoruš Z, Kovačević N. Arbutin content and antioxidant activity of some Ericaceae species. in Pharmazie. 2009;64(10):656-659.
doi:10.1691/ph.2009.9551 .

Pavlović, Dragana R., Lakušić, Branislava, Došlov-Kokoruš, Zvezdana, Kovačević, Nada, "Arbutin content and antioxidant activity of some Ericaceae species" in Pharmazie, 64, no. 10 (2009):656-659,
https://doi.org/10.1691/ph.2009.9551 . .

TY  - JOUR
AU  - Colić, M.
AU  - Došlov-Kokoruš, Zvezdana
AU  - Backović, A.
AU  - Stojanović, D.
AU  - Antić-Stanković, Jelena
AU  - Kovačević, Nada
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/989
AB  - The effect of the methanolic extract of the underground parts of Epimedium alpinum L. (MEEA) on the immune response to Keyhole Limpet Hemocyanine (KLH) or alloantigens in vivo was studied in AO rats. Immunization of experimental animals with KLH or allogeneic lymphocytes together with MEEA was followed by an increase in cellularity of draining lymph nodes (LN) and enhanced proliferation of LN lymphocytes after their restimulation with specific antigens in vitro, compared to control rats immunized without MEEA. These effects correlated with an increase in relative values of B, MHC class II+, CD25(+) and CD71(+) cells, whereas percentages of T cells and both subsets of T cells (CD4(+) and CD8(+)) were not significantly altered. As a consequence of higher LN cellularity, total numbers of all cell subsets in the MEEA-treated group of rats were significantly increased, compared to the corresponding control. The addition of MEEA together with KLH in vitro to LN lymphocytes of rats immunized with KLH or KLH and MEEA in vivo was manifested by significant increase (0.1 mu g/ml of MEEA) and decrease (50 mu g/ml and 100 mu g/ml of MEEA) of cell proliferation, respectively. However, when LN lymphocytes from rats, immunized in vivo with KLH and MEEA, were stimulated in vitro with MEEA together with an anti-alpha beta T cell receptor monoclonal antibody (R73), their proliferation was siginificantly inhibited. Taken together, obtained results suggest that MEEA possesses immunostimulatory activity in vivo, but some components from the extract exert immunosuppressive effects in vitro on previously in vivo activated T cells.
PB  - Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn
T2  - Pharmazie
T1  - Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo
VL  - 62
IS  - 9
SP  - 705
EP  - 708
UR  - https://hdl.handle.net/21.15107/rcub_farfar_989
ER  - 

@article{
author = "Colić, M. and Došlov-Kokoruš, Zvezdana and Backović, A. and Stojanović, D. and Antić-Stanković, Jelena and Kovačević, Nada",
year = "2007",
abstract = "The effect of the methanolic extract of the underground parts of Epimedium alpinum L. (MEEA) on the immune response to Keyhole Limpet Hemocyanine (KLH) or alloantigens in vivo was studied in AO rats. Immunization of experimental animals with KLH or allogeneic lymphocytes together with MEEA was followed by an increase in cellularity of draining lymph nodes (LN) and enhanced proliferation of LN lymphocytes after their restimulation with specific antigens in vitro, compared to control rats immunized without MEEA. These effects correlated with an increase in relative values of B, MHC class II+, CD25(+) and CD71(+) cells, whereas percentages of T cells and both subsets of T cells (CD4(+) and CD8(+)) were not significantly altered. As a consequence of higher LN cellularity, total numbers of all cell subsets in the MEEA-treated group of rats were significantly increased, compared to the corresponding control. The addition of MEEA together with KLH in vitro to LN lymphocytes of rats immunized with KLH or KLH and MEEA in vivo was manifested by significant increase (0.1 mu g/ml of MEEA) and decrease (50 mu g/ml and 100 mu g/ml of MEEA) of cell proliferation, respectively. However, when LN lymphocytes from rats, immunized in vivo with KLH and MEEA, were stimulated in vitro with MEEA together with an anti-alpha beta T cell receptor monoclonal antibody (R73), their proliferation was siginificantly inhibited. Taken together, obtained results suggest that MEEA possesses immunostimulatory activity in vivo, but some components from the extract exert immunosuppressive effects in vitro on previously in vivo activated T cells.",
publisher = "Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn",
journal = "Pharmazie",
title = "Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo",
volume = "62",
number = "9",
pages = "705-708",
url = "https://hdl.handle.net/21.15107/rcub_farfar_989"
}

Colić, M., Došlov-Kokoruš, Z., Backović, A., Stojanović, D., Antić-Stanković, J.,& Kovačević, N.. (2007). Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo. in Pharmazie
Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn., 62(9), 705-708.
https://hdl.handle.net/21.15107/rcub_farfar_989

Colić M, Došlov-Kokoruš Z, Backović A, Stojanović D, Antić-Stanković J, Kovačević N. Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo. in Pharmazie. 2007;62(9):705-708.
https://hdl.handle.net/21.15107/rcub_farfar_989 .

Colić, M., Došlov-Kokoruš, Zvezdana, Backović, A., Stojanović, D., Antić-Stanković, Jelena, Kovačević, Nada, "Evaluation of the stimulatory effect of Epimedium alpinum L. methanolic extract on the immune response in vivo" in Pharmazie, 62, no. 9 (2007):705-708,
https://hdl.handle.net/21.15107/rcub_farfar_989 .

TY  - JOUR
AU  - Došlov-Kokoruš, Zvezdana
AU  - Ivanović, Ivana D.
AU  - Simić, Milena
AU  - Vajs, Vlatka
AU  - Kovačević, Nada
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/885
AB  - The aporphine alkaloid magnoflorine, known as a constituent of Asian Epimedium species, was isolated for the first time from the wild growing European species, Epimedium alpinum L. Identification was done by comparison with literature data. The HPLC method was applied for the determination of the content of the alkaloid in methanolic extracts of plant material. The underground part of E. alpinum contains 1-2 % of magnoflorine, while its content in methanolic extracts was 9.2-11.8 %. The aerial part of the plant contains less alkaloid (0.06-0.12 %), while the magnoflorine content in methanolic extracts of the aerial part was 0.4-0.8 %. Probably, the high content of magnoflorine in the methanolic extracts of underground parts of E. alpinum influences its dose-dependent cytotoxic activity (300 μg/ml; 39 % survival of cells in an experimental K562 cell culture).
AB  - Aporfinski alkaloid magnoflorin, poznat kao sastojak drugih vrsta roda Epimedium, po prvi put je izolovan iz evropske vrste Epimedium alpinum L. i identifikovan poređenjem sa literaturnim podacima. Ovo jedinjenje je korišćeno kao standard u HPLC analizi metanolnih ekstrakata E. alpinum. Sadržaj magnoflorina je znatno viši u ekstraktima podzemnih organa (9,2-11,8%, što odgovara sadržaju od 1-2 % magnoflorina u biljnom materijalu), nego u ekstraktima nadzemnih organa prevolca (0,4-0,8%,što odgovara sadržaju od 0,06-0,12%magnoflorina u biljnom materijalu). Sasvim je verovatno da je potvrđena citotoksična aktivnost metanolnog ekstrakta podzemnih organa (300 μg/ml; 39 % preživelih ćelija K562 kulture maligno-transformisanih ćelija), kao i imunomodulatorna aktivnost, povezana sa visokom koncentracijom ovog alkaloida u ispitivanom ekstraktu.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - The HPLC determination of the content of magnoflorine in epimedium alpinum L
T1  - HPLC određivanje sadržaja magnoflorina u prevolcu, epimedium alpinum L
VL  - 71
IS  - 3
SP  - 251
EP  - 255
DO  - 10.2298/JSC0603251D
ER  - 

@article{
author = "Došlov-Kokoruš, Zvezdana and Ivanović, Ivana D. and Simić, Milena and Vajs, Vlatka and Kovačević, Nada",
year = "2006",
abstract = "The aporphine alkaloid magnoflorine, known as a constituent of Asian Epimedium species, was isolated for the first time from the wild growing European species, Epimedium alpinum L. Identification was done by comparison with literature data. The HPLC method was applied for the determination of the content of the alkaloid in methanolic extracts of plant material. The underground part of E. alpinum contains 1-2 % of magnoflorine, while its content in methanolic extracts was 9.2-11.8 %. The aerial part of the plant contains less alkaloid (0.06-0.12 %), while the magnoflorine content in methanolic extracts of the aerial part was 0.4-0.8 %. Probably, the high content of magnoflorine in the methanolic extracts of underground parts of E. alpinum influences its dose-dependent cytotoxic activity (300 μg/ml; 39 % survival of cells in an experimental K562 cell culture)., Aporfinski alkaloid magnoflorin, poznat kao sastojak drugih vrsta roda Epimedium, po prvi put je izolovan iz evropske vrste Epimedium alpinum L. i identifikovan poređenjem sa literaturnim podacima. Ovo jedinjenje je korišćeno kao standard u HPLC analizi metanolnih ekstrakata E. alpinum. Sadržaj magnoflorina je znatno viši u ekstraktima podzemnih organa (9,2-11,8%, što odgovara sadržaju od 1-2 % magnoflorina u biljnom materijalu), nego u ekstraktima nadzemnih organa prevolca (0,4-0,8%,što odgovara sadržaju od 0,06-0,12%magnoflorina u biljnom materijalu). Sasvim je verovatno da je potvrđena citotoksična aktivnost metanolnog ekstrakta podzemnih organa (300 μg/ml; 39 % preživelih ćelija K562 kulture maligno-transformisanih ćelija), kao i imunomodulatorna aktivnost, povezana sa visokom koncentracijom ovog alkaloida u ispitivanom ekstraktu.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "The HPLC determination of the content of magnoflorine in epimedium alpinum L, HPLC određivanje sadržaja magnoflorina u prevolcu, epimedium alpinum L",
volume = "71",
number = "3",
pages = "251-255",
doi = "10.2298/JSC0603251D"
}

Došlov-Kokoruš, Z., Ivanović, I. D., Simić, M., Vajs, V.,& Kovačević, N.. (2006). The HPLC determination of the content of magnoflorine in epimedium alpinum L. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 71(3), 251-255.
https://doi.org/10.2298/JSC0603251D

Došlov-Kokoruš Z, Ivanović ID, Simić M, Vajs V, Kovačević N. The HPLC determination of the content of magnoflorine in epimedium alpinum L. in Journal of the Serbian Chemical Society. 2006;71(3):251-255.
doi:10.2298/JSC0603251D .

Došlov-Kokoruš, Zvezdana, Ivanović, Ivana D., Simić, Milena, Vajs, Vlatka, Kovačević, Nada, "The HPLC determination of the content of magnoflorine in epimedium alpinum L" in Journal of the Serbian Chemical Society, 71, no. 3 (2006):251-255,
https://doi.org/10.2298/JSC0603251D . .

TY  - CONF
AU  - Došlov-Kokoruš, Zvezdana
AU  - Jovičić, D.
AU  - Ivetić, B.
AU  - Kovačević, Nada
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/770
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Determination of arbutin and hydroquinone in the leaves of Arctostaphyllos uva-ursi, Vaccinium spp. and Pyrus pyraster
T1  - Određivanje sadržaja arbutina i hidrohinona u listi planike (Arctostaphyllos uva-ursq, listu Vaccinium spp. i listu divlje kruške (Pyrus pyraster)
VL  - 56
IS  - 5
SP  - 918
EP  - 919
UR  - https://hdl.handle.net/21.15107/rcub_farfar_770
ER  - 

@conference{
author = "Došlov-Kokoruš, Zvezdana and Jovičić, D. and Ivetić, B. and Kovačević, Nada",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Determination of arbutin and hydroquinone in the leaves of Arctostaphyllos uva-ursi, Vaccinium spp. and Pyrus pyraster, Određivanje sadržaja arbutina i hidrohinona u listi planike (Arctostaphyllos uva-ursq, listu Vaccinium spp. i listu divlje kruške (Pyrus pyraster)",
volume = "56",
number = "5",
pages = "918-919",
url = "https://hdl.handle.net/21.15107/rcub_farfar_770"
}

Došlov-Kokoruš, Z., Jovičić, D., Ivetić, B.,& Kovačević, N.. (2006). Determination of arbutin and hydroquinone in the leaves of Arctostaphyllos uva-ursi, Vaccinium spp. and Pyrus pyraster. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 918-919.
https://hdl.handle.net/21.15107/rcub_farfar_770

Došlov-Kokoruš Z, Jovičić D, Ivetić B, Kovačević N. Determination of arbutin and hydroquinone in the leaves of Arctostaphyllos uva-ursi, Vaccinium spp. and Pyrus pyraster. in Arhiv za farmaciju. 2006;56(5):918-919.
https://hdl.handle.net/21.15107/rcub_farfar_770 .

Došlov-Kokoruš, Zvezdana, Jovičić, D., Ivetić, B., Kovačević, Nada, "Determination of arbutin and hydroquinone in the leaves of Arctostaphyllos uva-ursi, Vaccinium spp. and Pyrus pyraster" in Arhiv za farmaciju, 56, no. 5 (2006):918-919,
https://hdl.handle.net/21.15107/rcub_farfar_770 .

TY  - JOUR
AU  - Kovačević, Nada
AU  - Čolić, Miodrag
AU  - Backović, Aleksandar
AU  - Došlov-Kokoruš, Zvezdana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/674
AB  - The effect of the methanolic extract of root and rhizome of Epimedium alpinum (MEEA) on phenotype and functions of rat lymphocytes in vitro was studied. It has been found that MEEA at lower concentrations (0.1 mu g/ml and 1 mu g/ml) significantly enhanced proliferation of splenocytes and thymocytes triggered by concanavalin A (Con A), whereas higher concentrations of the extract (50 mu g/ ml-500 mu g/ml) were inhibitory. The stimulatory effect of MEEA on Con A-induced proliferation of splenocytes correlated with the up-regulation of interleukin-2 receptor alpha (IL-2R alpha) expression. In addition, increased production of IL-2 was observed when a blocking IL-2R alpha monoclonal antibody (mAb) was added to cell cultures. MEEA-suppressed proliferation of splenocytes was due to the inhibition of IL-2 production, the down-regulation of IL-2Ra expression and the induction of apoptosis. Cellular proliferation in the presence of inhibitory concentrations of MEEA higher than 50 mu g/ml could not be restored by the addition of exogenous IL-2.
PB  - Elsevier Science BV, Amsterdam
T2  - Fitoterapia
T1  - Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro
VL  - 77
IS  - 7-8
SP  - 561
EP  - 567
DO  - 10.1016/j.fitote.2006.09.008
ER  - 

@article{
author = "Kovačević, Nada and Čolić, Miodrag and Backović, Aleksandar and Došlov-Kokoruš, Zvezdana",
year = "2006",
abstract = "The effect of the methanolic extract of root and rhizome of Epimedium alpinum (MEEA) on phenotype and functions of rat lymphocytes in vitro was studied. It has been found that MEEA at lower concentrations (0.1 mu g/ml and 1 mu g/ml) significantly enhanced proliferation of splenocytes and thymocytes triggered by concanavalin A (Con A), whereas higher concentrations of the extract (50 mu g/ ml-500 mu g/ml) were inhibitory. The stimulatory effect of MEEA on Con A-induced proliferation of splenocytes correlated with the up-regulation of interleukin-2 receptor alpha (IL-2R alpha) expression. In addition, increased production of IL-2 was observed when a blocking IL-2R alpha monoclonal antibody (mAb) was added to cell cultures. MEEA-suppressed proliferation of splenocytes was due to the inhibition of IL-2 production, the down-regulation of IL-2Ra expression and the induction of apoptosis. Cellular proliferation in the presence of inhibitory concentrations of MEEA higher than 50 mu g/ml could not be restored by the addition of exogenous IL-2.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Fitoterapia",
title = "Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro",
volume = "77",
number = "7-8",
pages = "561-567",
doi = "10.1016/j.fitote.2006.09.008"
}

Kovačević, N., Čolić, M., Backović, A.,& Došlov-Kokoruš, Z.. (2006). Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro. in Fitoterapia
Elsevier Science BV, Amsterdam., 77(7-8), 561-567.
https://doi.org/10.1016/j.fitote.2006.09.008

Kovačević N, Čolić M, Backović A, Došlov-Kokoruš Z. Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro. in Fitoterapia. 2006;77(7-8):561-567.
doi:10.1016/j.fitote.2006.09.008 .

Kovačević, Nada, Čolić, Miodrag, Backović, Aleksandar, Došlov-Kokoruš, Zvezdana, "Immunomodulatory effects of the methanolic extract of Epimedium alpinum in vitro" in Fitoterapia, 77, no. 7-8 (2006):561-567,
https://doi.org/10.1016/j.fitote.2006.09.008 . .

TY  - CONF
AU  - Došlov-Kokoruš, Zvezdana
AU  - Pavlović, Milica
AU  - Simić, Mirjana
AU  - Kovačević, Nada
PY  - 2002
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/334
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Preliminary assay on the antioxidative activity of extracts of Epimedium alpinum L.
T1  - Preliminarno ispitivanje antioksidativne aktivnosti ekstrakata prevolca, Epimedium alpinum L.
VL  - 52
IS  - 4
SP  - 808
EP  - 809
UR  - https://hdl.handle.net/21.15107/rcub_farfar_334
ER  - 

@conference{
author = "Došlov-Kokoruš, Zvezdana and Pavlović, Milica and Simić, Mirjana and Kovačević, Nada",
year = "2002",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Preliminary assay on the antioxidative activity of extracts of Epimedium alpinum L., Preliminarno ispitivanje antioksidativne aktivnosti ekstrakata prevolca, Epimedium alpinum L.",
volume = "52",
number = "4",
pages = "808-809",
url = "https://hdl.handle.net/21.15107/rcub_farfar_334"
}

Došlov-Kokoruš, Z., Pavlović, M., Simić, M.,& Kovačević, N.. (2002). Preliminary assay on the antioxidative activity of extracts of Epimedium alpinum L.. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 52(4), 808-809.
https://hdl.handle.net/21.15107/rcub_farfar_334

Došlov-Kokoruš Z, Pavlović M, Simić M, Kovačević N. Preliminary assay on the antioxidative activity of extracts of Epimedium alpinum L.. in Arhiv za farmaciju. 2002;52(4):808-809.
https://hdl.handle.net/21.15107/rcub_farfar_334 .

Došlov-Kokoruš, Zvezdana, Pavlović, Milica, Simić, Mirjana, Kovačević, Nada, "Preliminary assay on the antioxidative activity of extracts of Epimedium alpinum L." in Arhiv za farmaciju, 52, no. 4 (2002):808-809,
https://hdl.handle.net/21.15107/rcub_farfar_334 .