TY  - JOUR
AU  - Malesević, M.
AU  - Živanović, Ljiljana
AU  - Protić, Ana
AU  - Radisić, Marina
AU  - Lausević, M.
AU  - Jović, Žarko
AU  - Zečević, Mira
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2150
AB  - The present study was designed to characterize the possible degradation products of zolpidem tartrate under various stress conditions according to International Conference on Harmonization (ICH) guidelines Q1A(R2). After exposure to light, heat, hydrolysis, and oxidation, the drug significantly degraded under photolytic and acid/base hydrolytic conditions. Degradation resulted in the formation of four key degradants. Degradation products were resolved from each other and the drug by employing an isocratic elution method on Luna C-18 column with mobile phase consisting of methanol-10 mM ammonium acetate (68.4: 31.6, v/v), wherein pH was adjusted to 5.4 with glacial acetic acid. To characterize the degradation products, a method was extended to LC-MS and a mass fragmentation pattern was established using single quad-rupole. The degradants were identified as zolpacid, oxozolpidem, zolpaldehyde, and zolpyridine. Finally, the most possible degradation mechanism of zolpidem tartrate in different environments was proposed.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Chromatographica
T1  - Stress Degradation Studies on Zolpidem Tartrate Using LC-DAD and LC-MS Methods
VL  - 26
IS  - 1
SP  - 81
EP  - 96
DO  - 10.1556/AChrom.26.2014.1.8
ER  - 

@article{
author = "Malesević, M. and Živanović, Ljiljana and Protić, Ana and Radisić, Marina and Lausević, M. and Jović, Žarko and Zečević, Mira",
year = "2014",
abstract = "The present study was designed to characterize the possible degradation products of zolpidem tartrate under various stress conditions according to International Conference on Harmonization (ICH) guidelines Q1A(R2). After exposure to light, heat, hydrolysis, and oxidation, the drug significantly degraded under photolytic and acid/base hydrolytic conditions. Degradation resulted in the formation of four key degradants. Degradation products were resolved from each other and the drug by employing an isocratic elution method on Luna C-18 column with mobile phase consisting of methanol-10 mM ammonium acetate (68.4: 31.6, v/v), wherein pH was adjusted to 5.4 with glacial acetic acid. To characterize the degradation products, a method was extended to LC-MS and a mass fragmentation pattern was established using single quad-rupole. The degradants were identified as zolpacid, oxozolpidem, zolpaldehyde, and zolpyridine. Finally, the most possible degradation mechanism of zolpidem tartrate in different environments was proposed.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Chromatographica",
title = "Stress Degradation Studies on Zolpidem Tartrate Using LC-DAD and LC-MS Methods",
volume = "26",
number = "1",
pages = "81-96",
doi = "10.1556/AChrom.26.2014.1.8"
}

Malesević, M., Živanović, L., Protić, A., Radisić, M., Lausević, M., Jović, Ž.,& Zečević, M.. (2014). Stress Degradation Studies on Zolpidem Tartrate Using LC-DAD and LC-MS Methods. in Acta Chromatographica
Akademiai Kiado Zrt, Budapest., 26(1), 81-96.
https://doi.org/10.1556/AChrom.26.2014.1.8

Malesević M, Živanović L, Protić A, Radisić M, Lausević M, Jović Ž, Zečević M. Stress Degradation Studies on Zolpidem Tartrate Using LC-DAD and LC-MS Methods. in Acta Chromatographica. 2014;26(1):81-96.
doi:10.1556/AChrom.26.2014.1.8 .

Malesević, M., Živanović, Ljiljana, Protić, Ana, Radisić, Marina, Lausević, M., Jović, Žarko, Zečević, Mira, "Stress Degradation Studies on Zolpidem Tartrate Using LC-DAD and LC-MS Methods" in Acta Chromatographica, 26, no. 1 (2014):81-96,
https://doi.org/10.1556/AChrom.26.2014.1.8 . .

TY  - JOUR
AU  - Jović, Žarko
AU  - Živanović, Ljiljana
AU  - Protić, Ana
AU  - Radisić, Marina
AU  - Lausević, Mila
AU  - Malešević, Marija
AU  - Zečević, Mira
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1961
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3431
AB  - Torasemide was subjected to forced degradation studies. Stress conditions were varied concerning hydrolysis (acid, base, and neutral), oxidation, photolysis, and thermal degradation in order to identify the potential degradation products and consequently establish the possible degradation pathways and intrinsic stability of the drug. The study was performed according to ICH guidelines and drug was found to be relatively stable in the solid form. It showed that torasemide degraded significantly under acidic, neutral and alkaline conditions and resulted in formation of degradation product R2. When temperature was increased the degradation was accelerated. Also, the drug showed slight instability under extreme oxidative stress conditions which resulted in formation of two degradation products in total. The drug and degradation products have been separated employing gradient elution method on Zorbax SB C-18 analytical column. To characterize the degradation products LC-MSn was applied. The mass fragmentation pattern was established using single quadrupole and ion trap mass analyzers. Finally, the most possible degradation mechanism of torasemide in different experimental conditions was proposed.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Forced degradation study of torasemide: Characterization of its degradation products
VL  - 36
IS  - 15
SP  - 2082
EP  - 2094
DO  - 10.1080/10826076.2012.712932
ER  - 

@article{
author = "Jović, Žarko and Živanović, Ljiljana and Protić, Ana and Radisić, Marina and Lausević, Mila and Malešević, Marija and Zečević, Mira",
year = "2013",
abstract = "Torasemide was subjected to forced degradation studies. Stress conditions were varied concerning hydrolysis (acid, base, and neutral), oxidation, photolysis, and thermal degradation in order to identify the potential degradation products and consequently establish the possible degradation pathways and intrinsic stability of the drug. The study was performed according to ICH guidelines and drug was found to be relatively stable in the solid form. It showed that torasemide degraded significantly under acidic, neutral and alkaline conditions and resulted in formation of degradation product R2. When temperature was increased the degradation was accelerated. Also, the drug showed slight instability under extreme oxidative stress conditions which resulted in formation of two degradation products in total. The drug and degradation products have been separated employing gradient elution method on Zorbax SB C-18 analytical column. To characterize the degradation products LC-MSn was applied. The mass fragmentation pattern was established using single quadrupole and ion trap mass analyzers. Finally, the most possible degradation mechanism of torasemide in different experimental conditions was proposed.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Forced degradation study of torasemide: Characterization of its degradation products",
volume = "36",
number = "15",
pages = "2082-2094",
doi = "10.1080/10826076.2012.712932"
}

Jović, Ž., Živanović, L., Protić, A., Radisić, M., Lausević, M., Malešević, M.,& Zečević, M.. (2013). Forced degradation study of torasemide: Characterization of its degradation products. in Journal of Liquid Chromatography & Related Technologies
Taylor & Francis Inc, Philadelphia., 36(15), 2082-2094.
https://doi.org/10.1080/10826076.2012.712932

Jović Ž, Živanović L, Protić A, Radisić M, Lausević M, Malešević M, Zečević M. Forced degradation study of torasemide: Characterization of its degradation products. in Journal of Liquid Chromatography & Related Technologies. 2013;36(15):2082-2094.
doi:10.1080/10826076.2012.712932 .

Jović, Žarko, Živanović, Ljiljana, Protić, Ana, Radisić, Marina, Lausević, Mila, Malešević, Marija, Zečević, Mira, "Forced degradation study of torasemide: Characterization of its degradation products" in Journal of Liquid Chromatography & Related Technologies, 36, no. 15 (2013):2082-2094,
https://doi.org/10.1080/10826076.2012.712932 . .

TY  - JOUR
AU  - Jović, Žarko
AU  - Živanović, Ljiljana
AU  - Protić, Ana
AU  - Radisić, Marina
AU  - Lausević, Mila
AU  - Malešević, Marija
AU  - Zečević, Mira
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1961
AB  - Torasemide was subjected to forced degradation studies. Stress conditions were varied concerning hydrolysis (acid, base, and neutral), oxidation, photolysis, and thermal degradation in order to identify the potential degradation products and consequently establish the possible degradation pathways and intrinsic stability of the drug. The study was performed according to ICH guidelines and drug was found to be relatively stable in the solid form. It showed that torasemide degraded significantly under acidic, neutral and alkaline conditions and resulted in formation of degradation product R2. When temperature was increased the degradation was accelerated. Also, the drug showed slight instability under extreme oxidative stress conditions which resulted in formation of two degradation products in total. The drug and degradation products have been separated employing gradient elution method on Zorbax SB C-18 analytical column. To characterize the degradation products LC-MSn was applied. The mass fragmentation pattern was established using single quadrupole and ion trap mass analyzers. Finally, the most possible degradation mechanism of torasemide in different experimental conditions was proposed.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Forced degradation study of torasemide: Characterization of its degradation products
VL  - 36
IS  - 15
SP  - 2082
EP  - 2094
DO  - 10.1080/10826076.2012.712932
ER  - 

@article{
author = "Jović, Žarko and Živanović, Ljiljana and Protić, Ana and Radisić, Marina and Lausević, Mila and Malešević, Marija and Zečević, Mira",
year = "2013",
abstract = "Torasemide was subjected to forced degradation studies. Stress conditions were varied concerning hydrolysis (acid, base, and neutral), oxidation, photolysis, and thermal degradation in order to identify the potential degradation products and consequently establish the possible degradation pathways and intrinsic stability of the drug. The study was performed according to ICH guidelines and drug was found to be relatively stable in the solid form. It showed that torasemide degraded significantly under acidic, neutral and alkaline conditions and resulted in formation of degradation product R2. When temperature was increased the degradation was accelerated. Also, the drug showed slight instability under extreme oxidative stress conditions which resulted in formation of two degradation products in total. The drug and degradation products have been separated employing gradient elution method on Zorbax SB C-18 analytical column. To characterize the degradation products LC-MSn was applied. The mass fragmentation pattern was established using single quadrupole and ion trap mass analyzers. Finally, the most possible degradation mechanism of torasemide in different experimental conditions was proposed.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Forced degradation study of torasemide: Characterization of its degradation products",
volume = "36",
number = "15",
pages = "2082-2094",
doi = "10.1080/10826076.2012.712932"
}

Jović, Ž., Živanović, L., Protić, A., Radisić, M., Lausević, M., Malešević, M.,& Zečević, M.. (2013). Forced degradation study of torasemide: Characterization of its degradation products. in Journal of Liquid Chromatography & Related Technologies
Taylor & Francis Inc, Philadelphia., 36(15), 2082-2094.
https://doi.org/10.1080/10826076.2012.712932

Jović Ž, Živanović L, Protić A, Radisić M, Lausević M, Malešević M, Zečević M. Forced degradation study of torasemide: Characterization of its degradation products. in Journal of Liquid Chromatography & Related Technologies. 2013;36(15):2082-2094.
doi:10.1080/10826076.2012.712932 .

Jović, Žarko, Živanović, Ljiljana, Protić, Ana, Radisić, Marina, Lausević, Mila, Malešević, Marija, Zečević, Mira, "Forced degradation study of torasemide: Characterization of its degradation products" in Journal of Liquid Chromatography & Related Technologies, 36, no. 15 (2013):2082-2094,
https://doi.org/10.1080/10826076.2012.712932 . .

TY  - JOUR
AU  - Protić, Ana
AU  - Otašević, Biljana
AU  - Golubović, Jelena
AU  - Zečević, Mira
AU  - Živanović, Ljiljana
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1809
AB  - Purification of biological matrix prior to HPLC analysis has been complex procedure and source of great variability of analytical results. The most used biological matrixes used for analysis are plasma, serum, urine and saliva and it has been advisable to use the simplest procedure for purification of these samples. Biological matrixes are complex and variability of its content is the main problem in development of bioanalytical methods. Namely, plasma and urine samples contain large number of endogenous compounds in concentrations much larger than concentration of investigated analyte. The concentrations of investigated analytes are often in very low concentrations and its structure can be very similar to structure of some endogenous compounds. Due to this problem, purification and concentration of biomatrix is one of the most important steps in development of bioanalytical methods. For bioanalytical methods the most important parameters are reliability and repeatability of the analytical results. Validation of bioanalytical chromatographic methods can be conducted according to The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH), Food and Drug Administration (FDA) and European Medicines Agency (EMA). During the validation process selectivity, limit of detection (LOD), lower limits of quantification (LLOQ), range, linearity, precision, accuracy, stability and efficacy of biological sample purification have to be investigated.
AB  - Priprema biološkog materijala pre HPLC analize predstavlja kompleksnu proceduru koja je obično izvor velike varijabilnosti dobijenih analitičkih rezultata. Najčešće analizirane telesne tečnosti jesu plazma, serum, urin i saliva, i poželjno je primeniti što jednostavniju proceduru pri pripremi navedenih uzoraka. Kompleksnost i varijabilnost sastava biološkog materijala predstavlja jedan od glavnih problema u razvoju bioanalitičkih metoda. Plazma i urin sadrže veliki broj endogenih komponenata prisutnih u koncentracijama koje su obično veće od koncentacije samog leka i/ili njegovih metabolita. Osim što se lekovi i/ili njihovi metaboliti često nalaze u malim koncentracijama, njihova struktura može biti slična strukturi nekih endogenih komponenata. Imajući ovo u vidu, prečišćavanje i koncentrisanje biološkog materijala je jedan od najvažnijih koraka u razvoju bioanalitičke metode. Bez obzira sa kojim se uzorcima biološkog materijala radi, važno je voditi računa da se tokom analize dobiju pouzdani i ponovljivi rezultati. Validacija bioanalitičkih hromatografskih metoda izvodi se prema preporukama The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) regulative, Food and Drug Administration (FDA) i European Medicines Agency (EMA). U okviru postupka validacije ispituje se selektivnost, donja granica detekcije (LD), donja granica određivanja (eng. lower limits of quantification, LLOQ), opseg, linearnost, preciznost, tačnost, stabilnost i efikasnost postupka prečišćavanja uzoraka biološkog materijala.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Purification of biological samples and validation of bioanalytical HPLC methods for analysis of drugs and their metabolites
T1  - Priprema biološkog materijala i validacija bioanalitičkih HPLC metoda za ispitivanje lekova i njihovih metabolite
VL  - 62
IS  - 6
SP  - 576
EP  - 586
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1809
ER  - 

@article{
author = "Protić, Ana and Otašević, Biljana and Golubović, Jelena and Zečević, Mira and Živanović, Ljiljana and Vekić, Jelena and Zeljković, Aleksandra",
year = "2012",
abstract = "Purification of biological matrix prior to HPLC analysis has been complex procedure and source of great variability of analytical results. The most used biological matrixes used for analysis are plasma, serum, urine and saliva and it has been advisable to use the simplest procedure for purification of these samples. Biological matrixes are complex and variability of its content is the main problem in development of bioanalytical methods. Namely, plasma and urine samples contain large number of endogenous compounds in concentrations much larger than concentration of investigated analyte. The concentrations of investigated analytes are often in very low concentrations and its structure can be very similar to structure of some endogenous compounds. Due to this problem, purification and concentration of biomatrix is one of the most important steps in development of bioanalytical methods. For bioanalytical methods the most important parameters are reliability and repeatability of the analytical results. Validation of bioanalytical chromatographic methods can be conducted according to The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH), Food and Drug Administration (FDA) and European Medicines Agency (EMA). During the validation process selectivity, limit of detection (LOD), lower limits of quantification (LLOQ), range, linearity, precision, accuracy, stability and efficacy of biological sample purification have to be investigated., Priprema biološkog materijala pre HPLC analize predstavlja kompleksnu proceduru koja je obično izvor velike varijabilnosti dobijenih analitičkih rezultata. Najčešće analizirane telesne tečnosti jesu plazma, serum, urin i saliva, i poželjno je primeniti što jednostavniju proceduru pri pripremi navedenih uzoraka. Kompleksnost i varijabilnost sastava biološkog materijala predstavlja jedan od glavnih problema u razvoju bioanalitičkih metoda. Plazma i urin sadrže veliki broj endogenih komponenata prisutnih u koncentracijama koje su obično veće od koncentacije samog leka i/ili njegovih metabolita. Osim što se lekovi i/ili njihovi metaboliti često nalaze u malim koncentracijama, njihova struktura može biti slična strukturi nekih endogenih komponenata. Imajući ovo u vidu, prečišćavanje i koncentrisanje biološkog materijala je jedan od najvažnijih koraka u razvoju bioanalitičke metode. Bez obzira sa kojim se uzorcima biološkog materijala radi, važno je voditi računa da se tokom analize dobiju pouzdani i ponovljivi rezultati. Validacija bioanalitičkih hromatografskih metoda izvodi se prema preporukama The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) regulative, Food and Drug Administration (FDA) i European Medicines Agency (EMA). U okviru postupka validacije ispituje se selektivnost, donja granica detekcije (LD), donja granica određivanja (eng. lower limits of quantification, LLOQ), opseg, linearnost, preciznost, tačnost, stabilnost i efikasnost postupka prečišćavanja uzoraka biološkog materijala.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Purification of biological samples and validation of bioanalytical HPLC methods for analysis of drugs and their metabolites, Priprema biološkog materijala i validacija bioanalitičkih HPLC metoda za ispitivanje lekova i njihovih metabolite",
volume = "62",
number = "6",
pages = "576-586",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1809"
}

Protić, A., Otašević, B., Golubović, J., Zečević, M., Živanović, L., Vekić, J.,& Zeljković, A.. (2012). Purification of biological samples and validation of bioanalytical HPLC methods for analysis of drugs and their metabolites. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 62(6), 576-586.
https://hdl.handle.net/21.15107/rcub_farfar_1809

Protić A, Otašević B, Golubović J, Zečević M, Živanović L, Vekić J, Zeljković A. Purification of biological samples and validation of bioanalytical HPLC methods for analysis of drugs and their metabolites. in Arhiv za farmaciju. 2012;62(6):576-586.
https://hdl.handle.net/21.15107/rcub_farfar_1809 .

Protić, Ana, Otašević, Biljana, Golubović, Jelena, Zečević, Mira, Živanović, Ljiljana, Vekić, Jelena, Zeljković, Aleksandra, "Purification of biological samples and validation of bioanalytical HPLC methods for analysis of drugs and their metabolites" in Arhiv za farmaciju, 62, no. 6 (2012):576-586,
https://hdl.handle.net/21.15107/rcub_farfar_1809 .

TY  - JOUR
AU  - Golubović, Jelena
AU  - Protić, Ana
AU  - Zečević, Mira
AU  - Otašević, Biljana
AU  - Mikić, Marija
AU  - Živanović, Ljiljana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1748
AB  - Artificial neural network (ANN) is a learning system based on a computational technique which can simulate the neurological processing ability of the human brain. It was employed for building of the quantitative structure-retention relationships (QSRRs) model of antifungal agents-imidazoles or triazoles by structure. Computed molecular descriptors together with the percentage of acetonitrile in mobile phase (v/v) and buffer pH, being the most influential HPLC factors, were used as network inputs, giving the retention factor as model output. The multilayer perceptron network with a 9-5-1 topology was trained by using the back propagation algorithm. Good correlation between experimentally obtained data and ones predicted by using QSRR-ANN on previously unseen data sets indicates good predictive ability of the model.
PB  - Elsevier Science BV, Amsterdam
T2  - Talanta
T1  - Quantitative structure retention relationships of azole antifungal agents in reversed-phase high performance liquid chromatography
VL  - 100
SP  - 329
EP  - 337
DO  - 10.1016/j.talanta.2012.07.071
ER  - 

@article{
author = "Golubović, Jelena and Protić, Ana and Zečević, Mira and Otašević, Biljana and Mikić, Marija and Živanović, Ljiljana",
year = "2012",
abstract = "Artificial neural network (ANN) is a learning system based on a computational technique which can simulate the neurological processing ability of the human brain. It was employed for building of the quantitative structure-retention relationships (QSRRs) model of antifungal agents-imidazoles or triazoles by structure. Computed molecular descriptors together with the percentage of acetonitrile in mobile phase (v/v) and buffer pH, being the most influential HPLC factors, were used as network inputs, giving the retention factor as model output. The multilayer perceptron network with a 9-5-1 topology was trained by using the back propagation algorithm. Good correlation between experimentally obtained data and ones predicted by using QSRR-ANN on previously unseen data sets indicates good predictive ability of the model.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Talanta",
title = "Quantitative structure retention relationships of azole antifungal agents in reversed-phase high performance liquid chromatography",
volume = "100",
pages = "329-337",
doi = "10.1016/j.talanta.2012.07.071"
}

Golubović, J., Protić, A., Zečević, M., Otašević, B., Mikić, M.,& Živanović, L.. (2012). Quantitative structure retention relationships of azole antifungal agents in reversed-phase high performance liquid chromatography. in Talanta
Elsevier Science BV, Amsterdam., 100, 329-337.
https://doi.org/10.1016/j.talanta.2012.07.071

Golubović J, Protić A, Zečević M, Otašević B, Mikić M, Živanović L. Quantitative structure retention relationships of azole antifungal agents in reversed-phase high performance liquid chromatography. in Talanta. 2012;100:329-337.
doi:10.1016/j.talanta.2012.07.071 .

Golubović, Jelena, Protić, Ana, Zečević, Mira, Otašević, Biljana, Mikić, Marija, Živanović, Ljiljana, "Quantitative structure retention relationships of azole antifungal agents in reversed-phase high performance liquid chromatography" in Talanta, 100 (2012):329-337,
https://doi.org/10.1016/j.talanta.2012.07.071 . .

TY  - JOUR
AU  - Milovanović, Svetlana
AU  - Otašević, Biljana
AU  - Zečević, Mira
AU  - Živanović, Ljiljana
AU  - Protić, Ana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1710
AB  - A simple, rapid, isocratic reversed-phase high-performance liquid chromatographic method was developed and validated for the analysis of moxonidine and its impurities in tablet formulations. The chromatographic separation was achieved on a Symmetry shield C18 column (250 mm x 4.6 mm, 5 mu m) by employing a mobile phase consisting of methanol-potassium phosphate buffer (0.05 M) mixture (15:85, v/v) (pH 3.5) at a flow rate of 1 ml min(-1): detection at 255 nm. Central composite design technique and response surface method were used to evaluate the effects of variations of selected factors (buffer pH value, column temperature, methanol content) in order to achieve the best isocratic separation within short analysis time (less than 10 min), as well as for robustness test considerations. The method fulfilled the validation criteria: specificity, linearity, accuracy, precision, limit of detection and limit of quantitation. The method was successfully applied for the analysis of commercial moxonidine tablets.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Development and validation of reversed phase high performance liquid chromatographic method for determination of moxonidine in the presence of its impurities
VL  - 59
SP  - 151
EP  - 156
DO  - 10.1016/j.jpba.2011.09.029
ER  - 

@article{
author = "Milovanović, Svetlana and Otašević, Biljana and Zečević, Mira and Živanović, Ljiljana and Protić, Ana",
year = "2012",
abstract = "A simple, rapid, isocratic reversed-phase high-performance liquid chromatographic method was developed and validated for the analysis of moxonidine and its impurities in tablet formulations. The chromatographic separation was achieved on a Symmetry shield C18 column (250 mm x 4.6 mm, 5 mu m) by employing a mobile phase consisting of methanol-potassium phosphate buffer (0.05 M) mixture (15:85, v/v) (pH 3.5) at a flow rate of 1 ml min(-1): detection at 255 nm. Central composite design technique and response surface method were used to evaluate the effects of variations of selected factors (buffer pH value, column temperature, methanol content) in order to achieve the best isocratic separation within short analysis time (less than 10 min), as well as for robustness test considerations. The method fulfilled the validation criteria: specificity, linearity, accuracy, precision, limit of detection and limit of quantitation. The method was successfully applied for the analysis of commercial moxonidine tablets.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Development and validation of reversed phase high performance liquid chromatographic method for determination of moxonidine in the presence of its impurities",
volume = "59",
pages = "151-156",
doi = "10.1016/j.jpba.2011.09.029"
}

Milovanović, S., Otašević, B., Zečević, M., Živanović, L.,& Protić, A.. (2012). Development and validation of reversed phase high performance liquid chromatographic method for determination of moxonidine in the presence of its impurities. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 59, 151-156.
https://doi.org/10.1016/j.jpba.2011.09.029

Milovanović S, Otašević B, Zečević M, Živanović L, Protić A. Development and validation of reversed phase high performance liquid chromatographic method for determination of moxonidine in the presence of its impurities. in Journal of Pharmaceutical and Biomedical Analysis. 2012;59:151-156.
doi:10.1016/j.jpba.2011.09.029 .

Milovanović, Svetlana, Otašević, Biljana, Zečević, Mira, Živanović, Ljiljana, Protić, Ana, "Development and validation of reversed phase high performance liquid chromatographic method for determination of moxonidine in the presence of its impurities" in Journal of Pharmaceutical and Biomedical Analysis, 59 (2012):151-156,
https://doi.org/10.1016/j.jpba.2011.09.029 . .

TY  - JOUR
AU  - Džodić, Predrag
AU  - Živanović, Ljiljana
AU  - Protić, Ana
AU  - Ivanović, Ivana
AU  - Veličković-Radovanović, Radmila
AU  - Spasić, Mirjana
AU  - Lukić, Stevo
AU  - Živanović, Slavoljub
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1823
AB  - A solid phase extraction-HPLC method has been developed and validated for the rapid analysis of carbamazepine and its two metabolites, carbamazepine epoxide and carbamazepine trans-diol, in human plasma. The analysis was performed using a C18 Bakerbond-BDC analytical column (250 mm×4.6 mm i.d., particle size 5 μm). The optimal conditions for the separation were established with the mobile phase acetonitrile - 10 mM phosphate buffer, pH 7.0 (30:70, v/v) at a flow rate of 1.5 mL min-1 and temperature of 35°C, with UV detection at 210 nm. The total run time was about 8 minutes. The SPE procedure for the extraction of the analytes from a plasma sample was developed using Oasis HLB cartridges and subsequently, the eluate was injected into the HPLC system for analysis. Afterwards, the SPE-HPLC method was subjected to validation. Linearity was obtained over the concentration range of 0.2-25 μg mL-1 for carbamazepine, carbamazepine epoxide and carbamazepine trans-diol, with correlation coefficients higher than 0.995. The method showed good intra-day and inter-day precision with a relative standard deviation below 7.96 %, while the accuracy ranged from 92.09 to 108.5 % for all analytes. Finally, the method was successfully applied to the analysis of the plasma samples of epileptic patients in mono- and polytherapy.
AB  - SPE-HPLC metoda je razvijena i validirana u cilju brzog analiziranja karbamazepina i metabolita karbamazepin epoksida i karbamazepin trans-diola u humanoj plazmi. C18 Bakerbond-BDC analitička kolona (250 mm x 4,6 mm; 5 μm) je korišćena radi izvođenja analize. Optimalni uslovi za hromatografsko razdvajanje su mobilna faza acetonitril - 10 mM fosfatni pufer, pH 7,0 (30:70, v/v), protok od 1,5 ml min-1, temperatura 35°C i detekcija na 210 nm. Ukupno trajanje hromatografskog rana iznosi oko 8 min. SPE procedura za ekstrakciju analita iz uzoraka plazme je razvijena uz korišćenje Oasis HLB ketridža nakon čega se eluat injektuje u HPLC sistem radi analiziranja. Zatim je izvršena validacija SPE-HPLC metode. Linearnost je potvrđena u koncentracionom opsegu 0,2-25 μg/ml za karbamazepin, karbamazepin epoksid i karbamazepin trans-diol sa vrednošću korelacionih koeficijenata višom od 0,995. Preciznost metode u toku jednog i u toku više dana je dobra sa relativnom standardnom devijacijom nižom od 7,96 %, dok tačnost metode obuhvata vrednosti u opsegu od 92,09 do 108,5 % za sve analite. Na kraju je metoda uspešno primenjena u cilju analiziranja uzoraka plazme pacijenata obolelih od epilepsije na monoterapiji i politerapiji.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Development and validation of a solid phase extraction-HPLC method for the determination of carbamazepine and its metabolites, carbamazepine epoxide and carbamazepine trans-diol, in plasma
T1  - Razvoj i validacija spe-HPLC metode za određivanje karbamazepina i metabolita karbamazepin epoksida i karbamazepin trans-diola u plazmi
VL  - 77
IS  - 10
SP  - 1423
EP  - 1436
DO  - 10.2298/JSC120106084D
ER  - 

@article{
author = "Džodić, Predrag and Živanović, Ljiljana and Protić, Ana and Ivanović, Ivana and Veličković-Radovanović, Radmila and Spasić, Mirjana and Lukić, Stevo and Živanović, Slavoljub",
year = "2012",
abstract = "A solid phase extraction-HPLC method has been developed and validated for the rapid analysis of carbamazepine and its two metabolites, carbamazepine epoxide and carbamazepine trans-diol, in human plasma. The analysis was performed using a C18 Bakerbond-BDC analytical column (250 mm×4.6 mm i.d., particle size 5 μm). The optimal conditions for the separation were established with the mobile phase acetonitrile - 10 mM phosphate buffer, pH 7.0 (30:70, v/v) at a flow rate of 1.5 mL min-1 and temperature of 35°C, with UV detection at 210 nm. The total run time was about 8 minutes. The SPE procedure for the extraction of the analytes from a plasma sample was developed using Oasis HLB cartridges and subsequently, the eluate was injected into the HPLC system for analysis. Afterwards, the SPE-HPLC method was subjected to validation. Linearity was obtained over the concentration range of 0.2-25 μg mL-1 for carbamazepine, carbamazepine epoxide and carbamazepine trans-diol, with correlation coefficients higher than 0.995. The method showed good intra-day and inter-day precision with a relative standard deviation below 7.96 %, while the accuracy ranged from 92.09 to 108.5 % for all analytes. Finally, the method was successfully applied to the analysis of the plasma samples of epileptic patients in mono- and polytherapy., SPE-HPLC metoda je razvijena i validirana u cilju brzog analiziranja karbamazepina i metabolita karbamazepin epoksida i karbamazepin trans-diola u humanoj plazmi. C18 Bakerbond-BDC analitička kolona (250 mm x 4,6 mm; 5 μm) je korišćena radi izvođenja analize. Optimalni uslovi za hromatografsko razdvajanje su mobilna faza acetonitril - 10 mM fosfatni pufer, pH 7,0 (30:70, v/v), protok od 1,5 ml min-1, temperatura 35°C i detekcija na 210 nm. Ukupno trajanje hromatografskog rana iznosi oko 8 min. SPE procedura za ekstrakciju analita iz uzoraka plazme je razvijena uz korišćenje Oasis HLB ketridža nakon čega se eluat injektuje u HPLC sistem radi analiziranja. Zatim je izvršena validacija SPE-HPLC metode. Linearnost je potvrđena u koncentracionom opsegu 0,2-25 μg/ml za karbamazepin, karbamazepin epoksid i karbamazepin trans-diol sa vrednošću korelacionih koeficijenata višom od 0,995. Preciznost metode u toku jednog i u toku više dana je dobra sa relativnom standardnom devijacijom nižom od 7,96 %, dok tačnost metode obuhvata vrednosti u opsegu od 92,09 do 108,5 % za sve analite. Na kraju je metoda uspešno primenjena u cilju analiziranja uzoraka plazme pacijenata obolelih od epilepsije na monoterapiji i politerapiji.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Development and validation of a solid phase extraction-HPLC method for the determination of carbamazepine and its metabolites, carbamazepine epoxide and carbamazepine trans-diol, in plasma, Razvoj i validacija spe-HPLC metode za određivanje karbamazepina i metabolita karbamazepin epoksida i karbamazepin trans-diola u plazmi",
volume = "77",
number = "10",
pages = "1423-1436",
doi = "10.2298/JSC120106084D"
}

Džodić, P., Živanović, L., Protić, A., Ivanović, I., Veličković-Radovanović, R., Spasić, M., Lukić, S.,& Živanović, S.. (2012). Development and validation of a solid phase extraction-HPLC method for the determination of carbamazepine and its metabolites, carbamazepine epoxide and carbamazepine trans-diol, in plasma. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 77(10), 1423-1436.
https://doi.org/10.2298/JSC120106084D

Džodić P, Živanović L, Protić A, Ivanović I, Veličković-Radovanović R, Spasić M, Lukić S, Živanović S. Development and validation of a solid phase extraction-HPLC method for the determination of carbamazepine and its metabolites, carbamazepine epoxide and carbamazepine trans-diol, in plasma. in Journal of the Serbian Chemical Society. 2012;77(10):1423-1436.
doi:10.2298/JSC120106084D .

Džodić, Predrag, Živanović, Ljiljana, Protić, Ana, Ivanović, Ivana, Veličković-Radovanović, Radmila, Spasić, Mirjana, Lukić, Stevo, Živanović, Slavoljub, "Development and validation of a solid phase extraction-HPLC method for the determination of carbamazepine and its metabolites, carbamazepine epoxide and carbamazepine trans-diol, in plasma" in Journal of the Serbian Chemical Society, 77, no. 10 (2012):1423-1436,
https://doi.org/10.2298/JSC120106084D . .

TY  - JOUR
AU  - Jović, Žarko
AU  - Živanović, Ljiljana
AU  - Radisić, Marina
AU  - Protić, Ana
AU  - Malesevic, Marija
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1700
PB  - Oxford Univ Press Inc, Cary
T2  - Journal of Chromatographic Science
T1  - Chemometrically Assisted Development and Validation of LC-UV and LC-MS Methods for Simultaneous Determination of Torasemide and its Impurities
VL  - 50
IS  - 4
SP  - 324
EP  - 334
DO  - 10.1093/chromsci/bms033
ER  - 

@article{
author = "Jović, Žarko and Živanović, Ljiljana and Radisić, Marina and Protić, Ana and Malesevic, Marija",
year = "2012",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Journal of Chromatographic Science",
title = "Chemometrically Assisted Development and Validation of LC-UV and LC-MS Methods for Simultaneous Determination of Torasemide and its Impurities",
volume = "50",
number = "4",
pages = "324-334",
doi = "10.1093/chromsci/bms033"
}

Jović, Ž., Živanović, L., Radisić, M., Protić, A.,& Malesevic, M.. (2012). Chemometrically Assisted Development and Validation of LC-UV and LC-MS Methods for Simultaneous Determination of Torasemide and its Impurities. in Journal of Chromatographic Science
Oxford Univ Press Inc, Cary., 50(4), 324-334.
https://doi.org/10.1093/chromsci/bms033

Jović Ž, Živanović L, Radisić M, Protić A, Malesevic M. Chemometrically Assisted Development and Validation of LC-UV and LC-MS Methods for Simultaneous Determination of Torasemide and its Impurities. in Journal of Chromatographic Science. 2012;50(4):324-334.
doi:10.1093/chromsci/bms033 .

Jović, Žarko, Živanović, Ljiljana, Radisić, Marina, Protić, Ana, Malesevic, Marija, "Chemometrically Assisted Development and Validation of LC-UV and LC-MS Methods for Simultaneous Determination of Torasemide and its Impurities" in Journal of Chromatographic Science, 50, no. 4 (2012):324-334,
https://doi.org/10.1093/chromsci/bms033 . .

TY  - JOUR
AU  - Protić, Ana
AU  - Živanović, Ljiljana
AU  - Radisić, Marina
AU  - Lusevic, Mila
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1559
AB  - Multicriteria decision making methodology in combination with experimental design has been applied for optimization of stability-indicating LC/DAD method of Mycophenolate mofetil and its degradation products. Via fractional factorial design variables with statistically significant impact on retention parameters of the investigated substances were defined and then thoroughly optimized toward four chromatographic responses combining central composition design and desirability function. The separation was achieved on Chromolith column (C18e, 100x4.6mm) with the mobile phase consisted of acetonitrile0.015M KH2PO4 (pH 4.4) (28:72, v/v). The flow rate of the mobile phase was 2mL/min and the detection was performed using PDA detector at 215nm. The method has been suitably modified for LC/MS/MS analysis. The ammonium-acetate buffer was used instead of phosphate buffer and pH of the water phase was adjusted with acetic acid. The flow rate was 1mLmin-1. The substance was subjected to stress conditions (0.01M NaOH, 1M HCl, 3-30% H2O2, 70 degrees C and day light). It resulted in formation of Mycophenolic acid and two oxidation products. The substance showed to be stable only towards photolysis. The LC/MS/MS values and fragmentation pattern of two unknown oxidation products were used in its characterization.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Liquid Chromatography & Related Technologies
T1  - Impurity profiling of mycophenolate mofetil with the assisstance of desirability function in method development
VL  - 34
IS  - 12
SP  - 1014
EP  - 1035
DO  - 10.1080/10826076.2011.569809
ER  - 

@article{
author = "Protić, Ana and Živanović, Ljiljana and Radisić, Marina and Lusevic, Mila",
year = "2011",
abstract = "Multicriteria decision making methodology in combination with experimental design has been applied for optimization of stability-indicating LC/DAD method of Mycophenolate mofetil and its degradation products. Via fractional factorial design variables with statistically significant impact on retention parameters of the investigated substances were defined and then thoroughly optimized toward four chromatographic responses combining central composition design and desirability function. The separation was achieved on Chromolith column (C18e, 100x4.6mm) with the mobile phase consisted of acetonitrile0.015M KH2PO4 (pH 4.4) (28:72, v/v). The flow rate of the mobile phase was 2mL/min and the detection was performed using PDA detector at 215nm. The method has been suitably modified for LC/MS/MS analysis. The ammonium-acetate buffer was used instead of phosphate buffer and pH of the water phase was adjusted with acetic acid. The flow rate was 1mLmin-1. The substance was subjected to stress conditions (0.01M NaOH, 1M HCl, 3-30% H2O2, 70 degrees C and day light). It resulted in formation of Mycophenolic acid and two oxidation products. The substance showed to be stable only towards photolysis. The LC/MS/MS values and fragmentation pattern of two unknown oxidation products were used in its characterization.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Impurity profiling of mycophenolate mofetil with the assisstance of desirability function in method development",
volume = "34",
number = "12",
pages = "1014-1035",
doi = "10.1080/10826076.2011.569809"
}

Protić, A., Živanović, L., Radisić, M.,& Lusevic, M.. (2011). Impurity profiling of mycophenolate mofetil with the assisstance of desirability function in method development. in Journal of Liquid Chromatography & Related Technologies
Taylor & Francis Inc, Philadelphia., 34(12), 1014-1035.
https://doi.org/10.1080/10826076.2011.569809

Protić A, Živanović L, Radisić M, Lusevic M. Impurity profiling of mycophenolate mofetil with the assisstance of desirability function in method development. in Journal of Liquid Chromatography & Related Technologies. 2011;34(12):1014-1035.
doi:10.1080/10826076.2011.569809 .

Protić, Ana, Živanović, Ljiljana, Radisić, Marina, Lusevic, Mila, "Impurity profiling of mycophenolate mofetil with the assisstance of desirability function in method development" in Journal of Liquid Chromatography & Related Technologies, 34, no. 12 (2011):1014-1035,
https://doi.org/10.1080/10826076.2011.569809 . .

TY  - JOUR
AU  - Malesevic, Marija
AU  - Živanović, Ljiljana
AU  - Protić, Ana
AU  - Jović, Žarko
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1485
AB  - Multiresponse optimization methodology in combination with experimental design was employed as a powerful technique for simultaneous optimization of input variables significant for evaluation of chromatographic behaviour of zolpidem tartrate, zolpacid, oxozolpidem, zolpyridine and zolpaldehyde towards various responses. In the first stage of the investigation fractional factorial design was used to decrease the number of variables that should be studied in detail. Among examined variables, pH of the mobile phase, percentage of organic modifier and buffer concentration showed to be statistically important and were consequently optimized with central composite design and Derringer's desirability function. Four responses were considered, the retention factors of zolpacid and zolpaldehyde (the first and last peak) and the resolutions between critical peaks. Optimal conditions included Luna C-18(2) analytical column (250 mm x 4.6 mm, 5 mu m particle size), mobile phase consisted of methanol-10 mM ammonium acetate (68.4:31.6, v/v, pH 5.4) and column temperature of 35 degrees C. The flow rate of the mobile phase was 1 mL min(-1) and the detection was performed at 254 nm. At the end, the method was successfully validated in accordance with ICH guideline and subsequently applied to the analysis of commercially available zolpidem tartrate tablets.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products
VL  - 74
IS  - 3-4
SP  - 197
EP  - 208
DO  - 10.1007/s10337-011-2064-9
ER  - 

@article{
author = "Malesevic, Marija and Živanović, Ljiljana and Protić, Ana and Jović, Žarko",
year = "2011",
abstract = "Multiresponse optimization methodology in combination with experimental design was employed as a powerful technique for simultaneous optimization of input variables significant for evaluation of chromatographic behaviour of zolpidem tartrate, zolpacid, oxozolpidem, zolpyridine and zolpaldehyde towards various responses. In the first stage of the investigation fractional factorial design was used to decrease the number of variables that should be studied in detail. Among examined variables, pH of the mobile phase, percentage of organic modifier and buffer concentration showed to be statistically important and were consequently optimized with central composite design and Derringer's desirability function. Four responses were considered, the retention factors of zolpacid and zolpaldehyde (the first and last peak) and the resolutions between critical peaks. Optimal conditions included Luna C-18(2) analytical column (250 mm x 4.6 mm, 5 mu m particle size), mobile phase consisted of methanol-10 mM ammonium acetate (68.4:31.6, v/v, pH 5.4) and column temperature of 35 degrees C. The flow rate of the mobile phase was 1 mL min(-1) and the detection was performed at 254 nm. At the end, the method was successfully validated in accordance with ICH guideline and subsequently applied to the analysis of commercially available zolpidem tartrate tablets.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products",
volume = "74",
number = "3-4",
pages = "197-208",
doi = "10.1007/s10337-011-2064-9"
}

Malesevic, M., Živanović, L., Protić, A.,& Jović, Ž.. (2011). Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products. in Chromatographia
Springer Heidelberg, Heidelberg., 74(3-4), 197-208.
https://doi.org/10.1007/s10337-011-2064-9

Malesevic M, Živanović L, Protić A, Jović Ž. Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products. in Chromatographia. 2011;74(3-4):197-208.
doi:10.1007/s10337-011-2064-9 .

Malesevic, Marija, Živanović, Ljiljana, Protić, Ana, Jović, Žarko, "Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products" in Chromatographia, 74, no. 3-4 (2011):197-208,
https://doi.org/10.1007/s10337-011-2064-9 . .

TY  - JOUR
AU  - Džodić, Predrag
AU  - Živanović, Ljiljana
AU  - Protić, Ana
AU  - Zečević, Mira
AU  - Jocić, Biljana
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1341
AB  - An accurate and precise RP-HPLC method was developed and validated for the determination of carbamazepine and its impurities iminostilbene and iminodibenzyl in a tablet formulation with fluphenazine as an internal standard. Buffer methanol (50 + 50, v/v) was used as the mobile phase. During validation, specificity, linearity, precision, accuracy, LOD, LOQ, and robustness of the method were tested. The method was proven to be specific against placebo interference. Linearity was evaluated over the concentration range of 100-500, 0.05-0.25, and 0.1-0.5 mu g/mL, and the r values were 0.9994, 0.9997, and 0.9979 for carbamazepine, iminostilbene, and iminodibenzyl, respectively. Intraday precision of the method was good, and RSD was below 2% for all analytes. The accuracy of the method ranged from 100.69 to 102.10, 99.76 to 102.66, and 99.26 to 100.08% for carbamazepine, iminostilbene, and iminodibenzyl, respectively. LOD was 0.0125, 0.025, and 0.05 mu g/mL and LOQ was 0.05, 0.05, and 0.1 mu g/mL for carbamazepine, iminostilbene, and iminodibenzyl, respectively. Robustness of the method was proven by using a chemometric approach. The method was successfully applied to the analysis of commercially available carbamazepine tablets and showed good repeatability, with RSD below 2%.
PB  - AOAC Int, Gaithersburg
T2  - Journal of AOAC International
T1  - Determination of Carbamazepine and Its Impurities Iminostilbene and Iminodibenzyl in Solid Dosage Form by Column High-Performance Liquid Chromatography
VL  - 93
IS  - 4
SP  - 1059
EP  - 1068
DO  - 10.1093/jaoac/93.4.1059
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1341
ER  - 

@article{
author = "Džodić, Predrag and Živanović, Ljiljana and Protić, Ana and Zečević, Mira and Jocić, Biljana",
year = "2010",
abstract = "An accurate and precise RP-HPLC method was developed and validated for the determination of carbamazepine and its impurities iminostilbene and iminodibenzyl in a tablet formulation with fluphenazine as an internal standard. Buffer methanol (50 + 50, v/v) was used as the mobile phase. During validation, specificity, linearity, precision, accuracy, LOD, LOQ, and robustness of the method were tested. The method was proven to be specific against placebo interference. Linearity was evaluated over the concentration range of 100-500, 0.05-0.25, and 0.1-0.5 mu g/mL, and the r values were 0.9994, 0.9997, and 0.9979 for carbamazepine, iminostilbene, and iminodibenzyl, respectively. Intraday precision of the method was good, and RSD was below 2% for all analytes. The accuracy of the method ranged from 100.69 to 102.10, 99.76 to 102.66, and 99.26 to 100.08% for carbamazepine, iminostilbene, and iminodibenzyl, respectively. LOD was 0.0125, 0.025, and 0.05 mu g/mL and LOQ was 0.05, 0.05, and 0.1 mu g/mL for carbamazepine, iminostilbene, and iminodibenzyl, respectively. Robustness of the method was proven by using a chemometric approach. The method was successfully applied to the analysis of commercially available carbamazepine tablets and showed good repeatability, with RSD below 2%.",
publisher = "AOAC Int, Gaithersburg",
journal = "Journal of AOAC International",
title = "Determination of Carbamazepine and Its Impurities Iminostilbene and Iminodibenzyl in Solid Dosage Form by Column High-Performance Liquid Chromatography",
volume = "93",
number = "4",
pages = "1059-1068",
doi = "10.1093/jaoac/93.4.1059",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1341"
}

Džodić, P., Živanović, L., Protić, A., Zečević, M.,& Jocić, B.. (2010). Determination of Carbamazepine and Its Impurities Iminostilbene and Iminodibenzyl in Solid Dosage Form by Column High-Performance Liquid Chromatography. in Journal of AOAC International
AOAC Int, Gaithersburg., 93(4), 1059-1068.
https://doi.org/10.1093/jaoac/93.4.1059
https://hdl.handle.net/21.15107/rcub_farfar_1341

Džodić P, Živanović L, Protić A, Zečević M, Jocić B. Determination of Carbamazepine and Its Impurities Iminostilbene and Iminodibenzyl in Solid Dosage Form by Column High-Performance Liquid Chromatography. in Journal of AOAC International. 2010;93(4):1059-1068.
doi:10.1093/jaoac/93.4.1059
https://hdl.handle.net/21.15107/rcub_farfar_1341 .

Džodić, Predrag, Živanović, Ljiljana, Protić, Ana, Zečević, Mira, Jocić, Biljana, "Determination of Carbamazepine and Its Impurities Iminostilbene and Iminodibenzyl in Solid Dosage Form by Column High-Performance Liquid Chromatography" in Journal of AOAC International, 93, no. 4 (2010):1059-1068,
https://doi.org/10.1093/jaoac/93.4.1059 .,
https://hdl.handle.net/21.15107/rcub_farfar_1341 .

TY  - JOUR
AU  - Jocić, Biljana
AU  - Zečević, Mira
AU  - Živanović, Ljiljana
AU  - Protić, Ana
AU  - Jadranin, Milka
AU  - Vajs, Vlatka
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1262
AB  - The objective of the present study was to report the stability profile of novel antimigrain drug Eletriptan hydrobromide based on the information obtained from forced degradation studies. The drug was subjected to acid (0.1-1 mol L-1 HCl), neutral and base (0.1-1 mol L-1 NaOH) hydrolysis and to oxidative decomposition (3-15% (v/v) H2O2). Photolysis and thermo degradation at 75 degrees C were carried out in methanol solution and in solid state with both Eletriptan hydrobromide bulk drug and the tablet formulation. The products formed under different stress conditions were investigated by LC and LC-MS. The experimental conditions for LC were chosen by employing experimental design and multicriteria decision making methodology. These powerful tools enabled the accomplishment of satisfactory resolution with the shortest possible analysis time. Analytes were separated on a C-18 column (XTerra (TM), 150 mm x 3.9 mm, 5 mu m) with the mobile phase composed of methanol-water solution of TEA (pH 6.52, 1%, v/v) (30:70, v/v) pumped at 1 mL min(-1) flow rate. The column temperature was set at 50 degrees C and the detection at 225 nm using DAD detector. The LC method was suitably modified for LC-MS analysis which was further used to characterize the arisen degradation products. The possible degradation pathway was outlined based on the results. The drug appeared to be instable towards every stress condition but oxidation. The stability was not jeopardized even under more exaggerated conditions such as increased temperature of the solutions to 75 degrees C, increased strength of acid/alkali solutions and prolonged testing period. Validation of the LC-DAD method was carried out in accordance with ICH guideline. The method met all required criteria and was applied when testing the commercially available tablets.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Study of forced degradation behavior of Eletriptan hydrobromide by LC and LC-MS and development of stability-indicating method
VL  - 50
IS  - 4
SP  - 622
EP  - 629
DO  - 10.1016/j.jpba.2009.01.034
ER  - 

@article{
author = "Jocić, Biljana and Zečević, Mira and Živanović, Ljiljana and Protić, Ana and Jadranin, Milka and Vajs, Vlatka",
year = "2009",
abstract = "The objective of the present study was to report the stability profile of novel antimigrain drug Eletriptan hydrobromide based on the information obtained from forced degradation studies. The drug was subjected to acid (0.1-1 mol L-1 HCl), neutral and base (0.1-1 mol L-1 NaOH) hydrolysis and to oxidative decomposition (3-15% (v/v) H2O2). Photolysis and thermo degradation at 75 degrees C were carried out in methanol solution and in solid state with both Eletriptan hydrobromide bulk drug and the tablet formulation. The products formed under different stress conditions were investigated by LC and LC-MS. The experimental conditions for LC were chosen by employing experimental design and multicriteria decision making methodology. These powerful tools enabled the accomplishment of satisfactory resolution with the shortest possible analysis time. Analytes were separated on a C-18 column (XTerra (TM), 150 mm x 3.9 mm, 5 mu m) with the mobile phase composed of methanol-water solution of TEA (pH 6.52, 1%, v/v) (30:70, v/v) pumped at 1 mL min(-1) flow rate. The column temperature was set at 50 degrees C and the detection at 225 nm using DAD detector. The LC method was suitably modified for LC-MS analysis which was further used to characterize the arisen degradation products. The possible degradation pathway was outlined based on the results. The drug appeared to be instable towards every stress condition but oxidation. The stability was not jeopardized even under more exaggerated conditions such as increased temperature of the solutions to 75 degrees C, increased strength of acid/alkali solutions and prolonged testing period. Validation of the LC-DAD method was carried out in accordance with ICH guideline. The method met all required criteria and was applied when testing the commercially available tablets.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Study of forced degradation behavior of Eletriptan hydrobromide by LC and LC-MS and development of stability-indicating method",
volume = "50",
number = "4",
pages = "622-629",
doi = "10.1016/j.jpba.2009.01.034"
}

Jocić, B., Zečević, M., Živanović, L., Protić, A., Jadranin, M.,& Vajs, V.. (2009). Study of forced degradation behavior of Eletriptan hydrobromide by LC and LC-MS and development of stability-indicating method. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 50(4), 622-629.
https://doi.org/10.1016/j.jpba.2009.01.034

Jocić B, Zečević M, Živanović L, Protić A, Jadranin M, Vajs V. Study of forced degradation behavior of Eletriptan hydrobromide by LC and LC-MS and development of stability-indicating method. in Journal of Pharmaceutical and Biomedical Analysis. 2009;50(4):622-629.
doi:10.1016/j.jpba.2009.01.034 .

Jocić, Biljana, Zečević, Mira, Živanović, Ljiljana, Protić, Ana, Jadranin, Milka, Vajs, Vlatka, "Study of forced degradation behavior of Eletriptan hydrobromide by LC and LC-MS and development of stability-indicating method" in Journal of Pharmaceutical and Biomedical Analysis, 50, no. 4 (2009):622-629,
https://doi.org/10.1016/j.jpba.2009.01.034 . .

TY  - JOUR
AU  - Živanović, Ljiljana
AU  - Protić, Ana
AU  - Zečević, Mira
AU  - Jocić, Biljana
AU  - Kostić, Mirjana
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1255
AB  - Multicriteria optimization methodology was applied for development of isocratic reversed-phased HPLC method for simultaneous determination of mycophenolic acid (MPA) and mycophenolic acid glucuronide (MPAG) in human urine and plasma. In the first stage of method development, pH value of the water phase. percentage of acetonitrile, temperature of the column and flow rate of the mobile phase were investigated using fractional factorial design. Afterwards, the optimal conditions were found employing central composite design and Derringer's desirability function. Two goals were considered, the retention factor of the MPAG to be in the range, between 0.8 and 1.118 which allowed well separation of MPAG from the urine and plasma peaks, and the shortest possible total analysis run time. Then, the obtained sigmoid functions were used to transform the optimization criteria into the desirability values. The satisfactory chromatographic conditions were obtained with mobile phase consisted of acetonitrile-phosphate buffer (pH 2.4; 0.04 M KH2PO4) (28:72, v/v). The separation was performed on C-18 Chromolith column (100 mm x 4.6 mm) with flow rate of 5 mL/min, the temperature of the column was 25 degrees C and the chosen wavelength for simultaneous determination of MPA and MPAG was 215 nm. The MPAG eluted at 0.552 min and the duration of run was 3.092 min. Afterwards, the method was subjected to validation. Linearity was observed over the concentration range of 1-50 mu g/mL for MPA and 1-500 mu g/mL for MPAG in urine and 1-60 mu g/mL for MPA and 1-70 mu g/mL for MPAG in plasma matrix. The method showed intra-day and inter-day precision with relative standard deviation lower then 5% and accuracy as recovery (%) between 100 +/- 5%.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Multicriteria optimization methodology in development of HPLC separation of mycophenolic acid and mycophenolic acid glucuronide in human urine and plasma
VL  - 50
IS  - 4
SP  - 640
EP  - 648
DO  - 10.1016/j.jpba.2008.09.052
ER  - 

@article{
author = "Živanović, Ljiljana and Protić, Ana and Zečević, Mira and Jocić, Biljana and Kostić, Mirjana",
year = "2009",
abstract = "Multicriteria optimization methodology was applied for development of isocratic reversed-phased HPLC method for simultaneous determination of mycophenolic acid (MPA) and mycophenolic acid glucuronide (MPAG) in human urine and plasma. In the first stage of method development, pH value of the water phase. percentage of acetonitrile, temperature of the column and flow rate of the mobile phase were investigated using fractional factorial design. Afterwards, the optimal conditions were found employing central composite design and Derringer's desirability function. Two goals were considered, the retention factor of the MPAG to be in the range, between 0.8 and 1.118 which allowed well separation of MPAG from the urine and plasma peaks, and the shortest possible total analysis run time. Then, the obtained sigmoid functions were used to transform the optimization criteria into the desirability values. The satisfactory chromatographic conditions were obtained with mobile phase consisted of acetonitrile-phosphate buffer (pH 2.4; 0.04 M KH2PO4) (28:72, v/v). The separation was performed on C-18 Chromolith column (100 mm x 4.6 mm) with flow rate of 5 mL/min, the temperature of the column was 25 degrees C and the chosen wavelength for simultaneous determination of MPA and MPAG was 215 nm. The MPAG eluted at 0.552 min and the duration of run was 3.092 min. Afterwards, the method was subjected to validation. Linearity was observed over the concentration range of 1-50 mu g/mL for MPA and 1-500 mu g/mL for MPAG in urine and 1-60 mu g/mL for MPA and 1-70 mu g/mL for MPAG in plasma matrix. The method showed intra-day and inter-day precision with relative standard deviation lower then 5% and accuracy as recovery (%) between 100 +/- 5%.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Multicriteria optimization methodology in development of HPLC separation of mycophenolic acid and mycophenolic acid glucuronide in human urine and plasma",
volume = "50",
number = "4",
pages = "640-648",
doi = "10.1016/j.jpba.2008.09.052"
}

Živanović, L., Protić, A., Zečević, M., Jocić, B.,& Kostić, M.. (2009). Multicriteria optimization methodology in development of HPLC separation of mycophenolic acid and mycophenolic acid glucuronide in human urine and plasma. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 50(4), 640-648.
https://doi.org/10.1016/j.jpba.2008.09.052

Živanović L, Protić A, Zečević M, Jocić B, Kostić M. Multicriteria optimization methodology in development of HPLC separation of mycophenolic acid and mycophenolic acid glucuronide in human urine and plasma. in Journal of Pharmaceutical and Biomedical Analysis. 2009;50(4):640-648.
doi:10.1016/j.jpba.2008.09.052 .

Živanović, Ljiljana, Protić, Ana, Zečević, Mira, Jocić, Biljana, Kostić, Mirjana, "Multicriteria optimization methodology in development of HPLC separation of mycophenolic acid and mycophenolic acid glucuronide in human urine and plasma" in Journal of Pharmaceutical and Biomedical Analysis, 50, no. 4 (2009):640-648,
https://doi.org/10.1016/j.jpba.2008.09.052 . .

TY  - JOUR
AU  - Protić, Ana
AU  - Živanović, Ljiljana
AU  - Zečević, Mira
AU  - Jocić, Biljana
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1243
PB  - Oxford Univ Press Inc, Cary
T2  - Journal of Chromatographic Science
T1  - Development of Liquid Chromatographic Method for Simultaneous Determination of Mycophenolate Mofetil and its Degradation Product Mycophenolic Acid in Dosage Form
VL  - 47
IS  - 2
SP  - 149
EP  - 155
DO  - 10.1093/chromsci/47.2.149
ER  - 

@article{
author = "Protić, Ana and Živanović, Ljiljana and Zečević, Mira and Jocić, Biljana",
year = "2009",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Journal of Chromatographic Science",
title = "Development of Liquid Chromatographic Method for Simultaneous Determination of Mycophenolate Mofetil and its Degradation Product Mycophenolic Acid in Dosage Form",
volume = "47",
number = "2",
pages = "149-155",
doi = "10.1093/chromsci/47.2.149"
}

Protić, A., Živanović, L., Zečević, M.,& Jocić, B.. (2009). Development of Liquid Chromatographic Method for Simultaneous Determination of Mycophenolate Mofetil and its Degradation Product Mycophenolic Acid in Dosage Form. in Journal of Chromatographic Science
Oxford Univ Press Inc, Cary., 47(2), 149-155.
https://doi.org/10.1093/chromsci/47.2.149

Protić A, Živanović L, Zečević M, Jocić B. Development of Liquid Chromatographic Method for Simultaneous Determination of Mycophenolate Mofetil and its Degradation Product Mycophenolic Acid in Dosage Form. in Journal of Chromatographic Science. 2009;47(2):149-155.
doi:10.1093/chromsci/47.2.149 .

Protić, Ana, Živanović, Ljiljana, Zečević, Mira, Jocić, Biljana, "Development of Liquid Chromatographic Method for Simultaneous Determination of Mycophenolate Mofetil and its Degradation Product Mycophenolic Acid in Dosage Form" in Journal of Chromatographic Science, 47, no. 2 (2009):149-155,
https://doi.org/10.1093/chromsci/47.2.149 . .

TY  - JOUR
AU  - Džodić, Predrag
AU  - Živanović, Ljiljana
AU  - Protić, Ana
AU  - Zečević, Mira
AU  - Jocić, Biljana
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1214
AB  - A chemometrical approach was applied to develop a reversed-phase liquid chromatographic method for simultaneous determination of carbamazepine and its impurities iminostilbene and iminodibenzyl in solid dosage form. According to contemporary literature, no method was developed for simultaneous determination of carbamazepine and these impurities by chemometrical approach. The fractional factorial design was used for selection of variables significantly influencing the chromatographic separation of the investigated substances. The investigated variables were: temperature of the column, the percentage of organic modifier, the acetate buffer concentration and pH of water phase. The first three variables were proved to be significant and were optimized by face centered, central composite design. Investigation was performed using C18 XBridge Shield analytical column (50 mm x 4.6 mm i.d., particle size 3.5 A mu m). The optimal conditions for the separation were established with the mobile phase composition of methanol-10 mM acetate buffer (pH adjusted to 2.21 with glacial acetic acid) (50:50, v/v) at a flow rate of 1.5 mL min(-1), 25 A degrees C column temperature and detection at 260 nm. Total analysis time was shortened to about 8 min. Finally, the method was successfully validated and subsequently applied to the analysis of commercially available carbamazepine tablets.
PB  - Springer Heidelberg, Heidelberg
T2  - Chromatographia
T1  - Chemometrically Assisted Development and Validation of LC for Simultaneous Determination of Carbamazepine and Its Impurities Iminostilbene and Iminodibenzyl in Solid Dosage Form
VL  - 70
IS  - 9-10
SP  - 1343
EP  - 1351
DO  - 10.1365/s10337-009-1322-6
ER  - 

@article{
author = "Džodić, Predrag and Živanović, Ljiljana and Protić, Ana and Zečević, Mira and Jocić, Biljana",
year = "2009",
abstract = "A chemometrical approach was applied to develop a reversed-phase liquid chromatographic method for simultaneous determination of carbamazepine and its impurities iminostilbene and iminodibenzyl in solid dosage form. According to contemporary literature, no method was developed for simultaneous determination of carbamazepine and these impurities by chemometrical approach. The fractional factorial design was used for selection of variables significantly influencing the chromatographic separation of the investigated substances. The investigated variables were: temperature of the column, the percentage of organic modifier, the acetate buffer concentration and pH of water phase. The first three variables were proved to be significant and were optimized by face centered, central composite design. Investigation was performed using C18 XBridge Shield analytical column (50 mm x 4.6 mm i.d., particle size 3.5 A mu m). The optimal conditions for the separation were established with the mobile phase composition of methanol-10 mM acetate buffer (pH adjusted to 2.21 with glacial acetic acid) (50:50, v/v) at a flow rate of 1.5 mL min(-1), 25 A degrees C column temperature and detection at 260 nm. Total analysis time was shortened to about 8 min. Finally, the method was successfully validated and subsequently applied to the analysis of commercially available carbamazepine tablets.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Chromatographia",
title = "Chemometrically Assisted Development and Validation of LC for Simultaneous Determination of Carbamazepine and Its Impurities Iminostilbene and Iminodibenzyl in Solid Dosage Form",
volume = "70",
number = "9-10",
pages = "1343-1351",
doi = "10.1365/s10337-009-1322-6"
}

Džodić, P., Živanović, L., Protić, A., Zečević, M.,& Jocić, B.. (2009). Chemometrically Assisted Development and Validation of LC for Simultaneous Determination of Carbamazepine and Its Impurities Iminostilbene and Iminodibenzyl in Solid Dosage Form. in Chromatographia
Springer Heidelberg, Heidelberg., 70(9-10), 1343-1351.
https://doi.org/10.1365/s10337-009-1322-6

Džodić P, Živanović L, Protić A, Zečević M, Jocić B. Chemometrically Assisted Development and Validation of LC for Simultaneous Determination of Carbamazepine and Its Impurities Iminostilbene and Iminodibenzyl in Solid Dosage Form. in Chromatographia. 2009;70(9-10):1343-1351.
doi:10.1365/s10337-009-1322-6 .

Džodić, Predrag, Živanović, Ljiljana, Protić, Ana, Zečević, Mira, Jocić, Biljana, "Chemometrically Assisted Development and Validation of LC for Simultaneous Determination of Carbamazepine and Its Impurities Iminostilbene and Iminodibenzyl in Solid Dosage Form" in Chromatographia, 70, no. 9-10 (2009):1343-1351,
https://doi.org/10.1365/s10337-009-1322-6 . .

TY  - JOUR
AU  - Zečević, Mira
AU  - Jocić, Biljana
AU  - Živanović, Ljiljana
AU  - Protić, Ana
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1062
AB  - The aim of this study was to establish an improved isocratic RP-LC-DAD method for separation and determination of rizatriptan benzoate and its two degradation products, L-749.019 and L-783.540 in tablets. Since the chromatographic behavior of target substances can be influenced by various experimental parameters, the whole study was carried out by employing experimental design methodology. The investigation included the influence of mutual changes of the mobile phase composition (methanol amount in the range 3-7% and pH of the water phase from 5.0 to 6.0) and the temperature (from 20 to 30 degrees C). The response surface design by means of Box-Brehnken design was used to obtain a predictive model which describes the changes in the response within the experimental domain. Additionally, several different target responses were evaluated and Derringer's desirability function was used for reaching a suitable compromise among the responses. This multicriteria decision making approach is based on constructing a desirability function for each individual response and afterwards establishing the overall desirability function. Such methodology provided us with the best operating conditions, satisfactory resolutions between the analytes and the shortest possible total analysis time. The experiments were performed on C(18) XTerra (150 mm 9 3.9 mm), 5 mu m column with the mobile phase consisting of a mixture of methanol, TEA and 0.01 mol L(-1) KH(2)PO(4) (6:9.4:84.6 v/v) pumped at a flow rate of 1.2 mL min(-1), pH of the water phase adjusted to 6 with 85% orthophosphoric acid, a column temperature of 20 degrees C and detection at 225 nm. Afterwards, the new method was validated and subsequently applied in analysis of commercially available rizatriptan tablets.
PB  - Vieweg, Wiesbaden
T2  - Chromatographia
T1  - Application of Multicriteria Methodology in the Development of Improved RP-LC-DAD for Determination of Rizatriptan and Its Degradation Products
VL  - 68
IS  - 11-12
SP  - 911
EP  - 918
DO  - 10.1365/s10337-008-0823-z
ER  - 

@article{
author = "Zečević, Mira and Jocić, Biljana and Živanović, Ljiljana and Protić, Ana",
year = "2008",
abstract = "The aim of this study was to establish an improved isocratic RP-LC-DAD method for separation and determination of rizatriptan benzoate and its two degradation products, L-749.019 and L-783.540 in tablets. Since the chromatographic behavior of target substances can be influenced by various experimental parameters, the whole study was carried out by employing experimental design methodology. The investigation included the influence of mutual changes of the mobile phase composition (methanol amount in the range 3-7% and pH of the water phase from 5.0 to 6.0) and the temperature (from 20 to 30 degrees C). The response surface design by means of Box-Brehnken design was used to obtain a predictive model which describes the changes in the response within the experimental domain. Additionally, several different target responses were evaluated and Derringer's desirability function was used for reaching a suitable compromise among the responses. This multicriteria decision making approach is based on constructing a desirability function for each individual response and afterwards establishing the overall desirability function. Such methodology provided us with the best operating conditions, satisfactory resolutions between the analytes and the shortest possible total analysis time. The experiments were performed on C(18) XTerra (150 mm 9 3.9 mm), 5 mu m column with the mobile phase consisting of a mixture of methanol, TEA and 0.01 mol L(-1) KH(2)PO(4) (6:9.4:84.6 v/v) pumped at a flow rate of 1.2 mL min(-1), pH of the water phase adjusted to 6 with 85% orthophosphoric acid, a column temperature of 20 degrees C and detection at 225 nm. Afterwards, the new method was validated and subsequently applied in analysis of commercially available rizatriptan tablets.",
publisher = "Vieweg, Wiesbaden",
journal = "Chromatographia",
title = "Application of Multicriteria Methodology in the Development of Improved RP-LC-DAD for Determination of Rizatriptan and Its Degradation Products",
volume = "68",
number = "11-12",
pages = "911-918",
doi = "10.1365/s10337-008-0823-z"
}

Zečević, M., Jocić, B., Živanović, L.,& Protić, A.. (2008). Application of Multicriteria Methodology in the Development of Improved RP-LC-DAD for Determination of Rizatriptan and Its Degradation Products. in Chromatographia
Vieweg, Wiesbaden., 68(11-12), 911-918.
https://doi.org/10.1365/s10337-008-0823-z

Zečević M, Jocić B, Živanović L, Protić A. Application of Multicriteria Methodology in the Development of Improved RP-LC-DAD for Determination of Rizatriptan and Its Degradation Products. in Chromatographia. 2008;68(11-12):911-918.
doi:10.1365/s10337-008-0823-z .

Zečević, Mira, Jocić, Biljana, Živanović, Ljiljana, Protić, Ana, "Application of Multicriteria Methodology in the Development of Improved RP-LC-DAD for Determination of Rizatriptan and Its Degradation Products" in Chromatographia, 68, no. 11-12 (2008):911-918,
https://doi.org/10.1365/s10337-008-0823-z . .

TY  - JOUR
AU  - Živanović, Ljiljana
AU  - Licanski, A.
AU  - Zečević, Mira
AU  - Jocić, Biljana
AU  - Kostić, Mirjana
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1089
AB  - The aim of this study was to develop and optimize a solid phase extraction (SPE) procedure for purification of mycophenolic acid (MPA) and its metabolite mycophenolic acid glucuronide (MPAG) in biological samples. During optimization process chemometric approach was applied. First, in screening experiments fractional factorial design (FFD) was used for selecting the variables which affected the extraction procedure. The ionic strength of the phosphate buffer in the washing step and the percentage of acetonitrile in the elution step were statistically significant for the recovery of MPAG while the percentage of acetonitrile and pH of the washing solution were statistically significant for that of MPA. Afterwards, the significant variables were optimized using central composite design (CCD). The developed SPE method included phosphate buffer (pH 2.4; 0.056 M) in the washing step, and the mixture of acetonitrile and phosphate buffer of which pH was adjusted to 2.4 (70:30, v/v) in the elution step. The investigation was applied to both urine and plasma and the nature of biological matrix appeared to be of no importance. The extraction from both matrixes showed good repeatability with relative standard deviations up to 6% for MPAG and 8% for MPA, and recovery around 100% for both substances. Furthermore, new SPE-RP-HPLC method for determination of MPA and MPAG in both humane urine and plasma has been validated. The great advantage of this method is the chromatographic run of only 3 min.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation
VL  - 47
IS  - 3
SP  - 575
EP  - 585
DO  - 10.1016/j.jpba.2008.01.046
ER  - 

@article{
author = "Živanović, Ljiljana and Licanski, A. and Zečević, Mira and Jocić, Biljana and Kostić, Mirjana",
year = "2008",
abstract = "The aim of this study was to develop and optimize a solid phase extraction (SPE) procedure for purification of mycophenolic acid (MPA) and its metabolite mycophenolic acid glucuronide (MPAG) in biological samples. During optimization process chemometric approach was applied. First, in screening experiments fractional factorial design (FFD) was used for selecting the variables which affected the extraction procedure. The ionic strength of the phosphate buffer in the washing step and the percentage of acetonitrile in the elution step were statistically significant for the recovery of MPAG while the percentage of acetonitrile and pH of the washing solution were statistically significant for that of MPA. Afterwards, the significant variables were optimized using central composite design (CCD). The developed SPE method included phosphate buffer (pH 2.4; 0.056 M) in the washing step, and the mixture of acetonitrile and phosphate buffer of which pH was adjusted to 2.4 (70:30, v/v) in the elution step. The investigation was applied to both urine and plasma and the nature of biological matrix appeared to be of no importance. The extraction from both matrixes showed good repeatability with relative standard deviations up to 6% for MPAG and 8% for MPA, and recovery around 100% for both substances. Furthermore, new SPE-RP-HPLC method for determination of MPA and MPAG in both humane urine and plasma has been validated. The great advantage of this method is the chromatographic run of only 3 min.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation",
volume = "47",
number = "3",
pages = "575-585",
doi = "10.1016/j.jpba.2008.01.046"
}

Živanović, L., Licanski, A., Zečević, M., Jocić, B.,& Kostić, M.. (2008). Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 47(3), 575-585.
https://doi.org/10.1016/j.jpba.2008.01.046

Živanović L, Licanski A, Zečević M, Jocić B, Kostić M. Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation. in Journal of Pharmaceutical and Biomedical Analysis. 2008;47(3):575-585.
doi:10.1016/j.jpba.2008.01.046 .

Živanović, Ljiljana, Licanski, A., Zečević, Mira, Jocić, Biljana, Kostić, Mirjana, "Application of experimental design in optimization of solid phase extraction of mycophenolic acid and mycophenolic acid glucuronide from human urine and plasma and SPE-RP-HPLC method validation" in Journal of Pharmaceutical and Biomedical Analysis, 47, no. 3 (2008):575-585,
https://doi.org/10.1016/j.jpba.2008.01.046 . .

TY  - CONF
AU  - Zečević, Mira
AU  - Živanović, Ljiljana
AU  - Jocić, Biljana
AU  - Ličanski, A.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/728
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - RP-HPLC method for simultaneous determination of active drugs and preservatives in Delmeson® ointment
T1  - RP-HPLC metoda za istovremenu analizu aktivnih komponenti i konzervanasa u Delmeson® masti
VL  - 56
IS  - 5
SP  - 756
EP  - 757
UR  - https://hdl.handle.net/21.15107/rcub_farfar_728
ER  - 

@conference{
author = "Zečević, Mira and Živanović, Ljiljana and Jocić, Biljana and Ličanski, A.",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "RP-HPLC method for simultaneous determination of active drugs and preservatives in Delmeson® ointment, RP-HPLC metoda za istovremenu analizu aktivnih komponenti i konzervanasa u Delmeson® masti",
volume = "56",
number = "5",
pages = "756-757",
url = "https://hdl.handle.net/21.15107/rcub_farfar_728"
}

Zečević, M., Živanović, L., Jocić, B.,& Ličanski, A.. (2006). RP-HPLC method for simultaneous determination of active drugs and preservatives in Delmeson® ointment. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 756-757.
https://hdl.handle.net/21.15107/rcub_farfar_728

Zečević M, Živanović L, Jocić B, Ličanski A. RP-HPLC method for simultaneous determination of active drugs and preservatives in Delmeson® ointment. in Arhiv za farmaciju. 2006;56(5):756-757.
https://hdl.handle.net/21.15107/rcub_farfar_728 .

Zečević, Mira, Živanović, Ljiljana, Jocić, Biljana, Ličanski, A., "RP-HPLC method for simultaneous determination of active drugs and preservatives in Delmeson® ointment" in Arhiv za farmaciju, 56, no. 5 (2006):756-757,
https://hdl.handle.net/21.15107/rcub_farfar_728 .

TY  - CONF
AU  - Zečević, Mira
AU  - Jocić, Biljana
AU  - Živanović, Ljiljana
AU  - Ličanski, A.
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/717
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - The preliminary investigations of the chromatografic behavior of eletriptan and rizatriptan in the reversed-phase system
T1  - Preliminarno ispitivanje hromatografskog ponašanja eletriptana i rizatriptana u reverzno-faznom sistemu
VL  - 56
IS  - 5
SP  - 754
EP  - 755
UR  - https://hdl.handle.net/21.15107/rcub_farfar_717
ER  - 

@conference{
author = "Zečević, Mira and Jocić, Biljana and Živanović, Ljiljana and Ličanski, A.",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "The preliminary investigations of the chromatografic behavior of eletriptan and rizatriptan in the reversed-phase system, Preliminarno ispitivanje hromatografskog ponašanja eletriptana i rizatriptana u reverzno-faznom sistemu",
volume = "56",
number = "5",
pages = "754-755",
url = "https://hdl.handle.net/21.15107/rcub_farfar_717"
}

Zečević, M., Jocić, B., Živanović, L.,& Ličanski, A.. (2006). The preliminary investigations of the chromatografic behavior of eletriptan and rizatriptan in the reversed-phase system. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 754-755.
https://hdl.handle.net/21.15107/rcub_farfar_717

Zečević M, Jocić B, Živanović L, Ličanski A. The preliminary investigations of the chromatografic behavior of eletriptan and rizatriptan in the reversed-phase system. in Arhiv za farmaciju. 2006;56(5):754-755.
https://hdl.handle.net/21.15107/rcub_farfar_717 .

Zečević, Mira, Jocić, Biljana, Živanović, Ljiljana, Ličanski, A., "The preliminary investigations of the chromatografic behavior of eletriptan and rizatriptan in the reversed-phase system" in Arhiv za farmaciju, 56, no. 5 (2006):754-755,
https://hdl.handle.net/21.15107/rcub_farfar_717 .

TY  - CONF
AU  - Živanović, Ljiljana
AU  - Ličanski, A.
AU  - Zečević, Mira
AU  - Jocić, Biljana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/756
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - RP-HPLC method for simultaneous determination of retinol acetate and ergocalciferol in oil oral drops
T1  - RP-HPLC metoda za istovremeno određivanje retinol acetata i ergokalciferola u uljanim kapima za oralnu primenu
VL  - 56
IS  - 5
SP  - 720
EP  - 721
UR  - https://hdl.handle.net/21.15107/rcub_farfar_756
ER  - 

@conference{
author = "Živanović, Ljiljana and Ličanski, A. and Zečević, Mira and Jocić, Biljana",
year = "2006",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "RP-HPLC method for simultaneous determination of retinol acetate and ergocalciferol in oil oral drops, RP-HPLC metoda za istovremeno određivanje retinol acetata i ergokalciferola u uljanim kapima za oralnu primenu",
volume = "56",
number = "5",
pages = "720-721",
url = "https://hdl.handle.net/21.15107/rcub_farfar_756"
}

Živanović, L., Ličanski, A., Zečević, M.,& Jocić, B.. (2006). RP-HPLC method for simultaneous determination of retinol acetate and ergocalciferol in oil oral drops. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 56(5), 720-721.
https://hdl.handle.net/21.15107/rcub_farfar_756

Živanović L, Ličanski A, Zečević M, Jocić B. RP-HPLC method for simultaneous determination of retinol acetate and ergocalciferol in oil oral drops. in Arhiv za farmaciju. 2006;56(5):720-721.
https://hdl.handle.net/21.15107/rcub_farfar_756 .

Živanović, Ljiljana, Ličanski, A., Zečević, Mira, Jocić, Biljana, "RP-HPLC method for simultaneous determination of retinol acetate and ergocalciferol in oil oral drops" in Arhiv za farmaciju, 56, no. 5 (2006):720-721,
https://hdl.handle.net/21.15107/rcub_farfar_756 .

TY  - JOUR
AU  - Zečević, Mira
AU  - Jocić, Biljana
AU  - Agatonović-Kuštrin, Snežana
AU  - Živanović, Ljiljana
PY  - 2006
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/878
AB  - Arapid and sensitive RPHPLCmethod was developed for the routine control analysis of eletriptan hydrobromide and its organic impurity UK 120.413 in Relpax® tablets. The chromatography was performed at 20°C using a C18 XTerra ™ (5 µm, 150 x 4,6 mm) column at a flow rate 1.0 ml/min. The drug and its impurity were detected at 225 nm. The mobile phase consisted of TEA (1 %) - methanol (67.2:32.8 v/v), the pH of which was adjusted to 6.8 with 85 % orthophosphoric acid. Quantification was accomplished by the internal standard method. The developed RP HPLC method was validated by testing: accuracy, precision, repeatability, specificity, detection limit, quantification limit, linearity, robustness and sensitivity. High linearity of the analytical procedure was confirmed over the concentration range of 0.05 - 1.00 mg/ml for eletriptan hydrobromide and from 0.10 - 1.50 µg/ml for UK 120.413, with correlation coefficients greater than r = 0.995. The low value of the RSD expressed the good repeatability and precision of the method. Experimental design and a response surface method were used to test robustness of the analytical procedure and to evaluate the effect of variation of the method parameters, namely the mobile phase composition, pH and temperature. They showed small deviations from the method setting. The good recovery and low RSD confirm the suitability of the proposed RP HPLC method for the routine determination of eletriptan hydrobromide and its impurity UK 120.413 in Relpax® tables.
AB  - U ovom radu je predstavljena brza i osetljiva RP HPLC metoda namenjena za rutinsko ispitivanje i kontrolu eletriptan hidrobromida i njegove organske nečistoće UK 120.413 u Relpax® tabletama. Hromatografski postupak je izveden uz korišćenje kolone C 18 XTerra ™ (5 µm, 150 x 4.6 mm) pri protoku mobilne faze od 1.0 ml/min i na 20 °C, a detekcija lekovite supstance i njene nečistoće je vršena na 225 nm. Mobilna faza se sastojala iz smeše TEA(1 %) - metanol (67,2:32,8 v/v), pH vodene faze je podešen na 6.8 sa 85 % ortofosfornom kiselinom. Kvantitativna analiza je vršena metodom internog standarda. Predložena RP HPLC metoda je validirana, a ispitivani su tačnost, preciznost, ponovljivost, specifičnost, limit detekcije, limit kvantifikacije, linearnost, robusnost i osetljivost metode. Visoka linearnost analitičkog postupka je potvrđena u opsegu koncentracija 0,05-1,00 mg/ml za eletriptan hidrobromid i 0,10-1,50 µg/ml za UK 120.413, sa koeficijentima korelacije koji su veći od r = 0,995. Niska vrednost relativne standardne devijacije potvrđuje dobru ponovljivost i preciznost metode. Eksperimentalni dizajn i metoda površine odgovora sistema su korišćeni u toku ispitivanja robusnosti da bi se procenio uticaj variranja vrednosti hromatografskih parametara metode. Test robusnosti je obuhvatao sastav mobilne faze, pH i temperaturu u malim variranjima vrednosti oko nominalne. Dobre "recovery" vrednosti i niska relativna standardna devijacija potvrđuju da je predložena RP HPLC pogodna za rutinsko određivanje eletriptan hidrobromida i njegove nečistoće UK 120.412 u Relpax® tabletama.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Validation of an HPLC method for the simultaneous determination of eletriptan and UK 120.413
T1  - Validacija HPLC metode za istovremeno određivanje eletriptana i UK 120.413
VL  - 71
IS  - 11
SP  - 1195
EP  - 1205
DO  - 10.2298/JSC0611195Z
ER  - 

@article{
author = "Zečević, Mira and Jocić, Biljana and Agatonović-Kuštrin, Snežana and Živanović, Ljiljana",
year = "2006",
abstract = "Arapid and sensitive RPHPLCmethod was developed for the routine control analysis of eletriptan hydrobromide and its organic impurity UK 120.413 in Relpax® tablets. The chromatography was performed at 20°C using a C18 XTerra ™ (5 µm, 150 x 4,6 mm) column at a flow rate 1.0 ml/min. The drug and its impurity were detected at 225 nm. The mobile phase consisted of TEA (1 %) - methanol (67.2:32.8 v/v), the pH of which was adjusted to 6.8 with 85 % orthophosphoric acid. Quantification was accomplished by the internal standard method. The developed RP HPLC method was validated by testing: accuracy, precision, repeatability, specificity, detection limit, quantification limit, linearity, robustness and sensitivity. High linearity of the analytical procedure was confirmed over the concentration range of 0.05 - 1.00 mg/ml for eletriptan hydrobromide and from 0.10 - 1.50 µg/ml for UK 120.413, with correlation coefficients greater than r = 0.995. The low value of the RSD expressed the good repeatability and precision of the method. Experimental design and a response surface method were used to test robustness of the analytical procedure and to evaluate the effect of variation of the method parameters, namely the mobile phase composition, pH and temperature. They showed small deviations from the method setting. The good recovery and low RSD confirm the suitability of the proposed RP HPLC method for the routine determination of eletriptan hydrobromide and its impurity UK 120.413 in Relpax® tables., U ovom radu je predstavljena brza i osetljiva RP HPLC metoda namenjena za rutinsko ispitivanje i kontrolu eletriptan hidrobromida i njegove organske nečistoće UK 120.413 u Relpax® tabletama. Hromatografski postupak je izveden uz korišćenje kolone C 18 XTerra ™ (5 µm, 150 x 4.6 mm) pri protoku mobilne faze od 1.0 ml/min i na 20 °C, a detekcija lekovite supstance i njene nečistoće je vršena na 225 nm. Mobilna faza se sastojala iz smeše TEA(1 %) - metanol (67,2:32,8 v/v), pH vodene faze je podešen na 6.8 sa 85 % ortofosfornom kiselinom. Kvantitativna analiza je vršena metodom internog standarda. Predložena RP HPLC metoda je validirana, a ispitivani su tačnost, preciznost, ponovljivost, specifičnost, limit detekcije, limit kvantifikacije, linearnost, robusnost i osetljivost metode. Visoka linearnost analitičkog postupka je potvrđena u opsegu koncentracija 0,05-1,00 mg/ml za eletriptan hidrobromid i 0,10-1,50 µg/ml za UK 120.413, sa koeficijentima korelacije koji su veći od r = 0,995. Niska vrednost relativne standardne devijacije potvrđuje dobru ponovljivost i preciznost metode. Eksperimentalni dizajn i metoda površine odgovora sistema su korišćeni u toku ispitivanja robusnosti da bi se procenio uticaj variranja vrednosti hromatografskih parametara metode. Test robusnosti je obuhvatao sastav mobilne faze, pH i temperaturu u malim variranjima vrednosti oko nominalne. Dobre "recovery" vrednosti i niska relativna standardna devijacija potvrđuju da je predložena RP HPLC pogodna za rutinsko određivanje eletriptan hidrobromida i njegove nečistoće UK 120.412 u Relpax® tabletama.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Validation of an HPLC method for the simultaneous determination of eletriptan and UK 120.413, Validacija HPLC metode za istovremeno određivanje eletriptana i UK 120.413",
volume = "71",
number = "11",
pages = "1195-1205",
doi = "10.2298/JSC0611195Z"
}

Zečević, M., Jocić, B., Agatonović-Kuštrin, S.,& Živanović, L.. (2006). Validation of an HPLC method for the simultaneous determination of eletriptan and UK 120.413. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 71(11), 1195-1205.
https://doi.org/10.2298/JSC0611195Z

Zečević M, Jocić B, Agatonović-Kuštrin S, Živanović L. Validation of an HPLC method for the simultaneous determination of eletriptan and UK 120.413. in Journal of the Serbian Chemical Society. 2006;71(11):1195-1205.
doi:10.2298/JSC0611195Z .

Zečević, Mira, Jocić, Biljana, Agatonović-Kuštrin, Snežana, Živanović, Ljiljana, "Validation of an HPLC method for the simultaneous determination of eletriptan and UK 120.413" in Journal of the Serbian Chemical Society, 71, no. 11 (2006):1195-1205,
https://doi.org/10.2298/JSC0611195Z . .

TY  - CONF
AU  - Zečević, Mira
AU  - Živanović, Ljiljana
PY  - 2002
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/348
PB  - Savez farmaceutskih udruženja Srbije, Beograd
C3  - Arhiv za farmaciju
T1  - Statistical optimization applied to the HPLC study of methiclothiazide and amiloride in tablets
T1  - Statistička optimizacija u HPLC analizi metiklotiazida i amilorida
VL  - 52
IS  - 4
SP  - 470
EP  - 471
UR  - https://hdl.handle.net/21.15107/rcub_farfar_348
ER  - 

@conference{
author = "Zečević, Mira and Živanović, Ljiljana",
year = "2002",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Statistical optimization applied to the HPLC study of methiclothiazide and amiloride in tablets, Statistička optimizacija u HPLC analizi metiklotiazida i amilorida",
volume = "52",
number = "4",
pages = "470-471",
url = "https://hdl.handle.net/21.15107/rcub_farfar_348"
}

Zečević, M.,& Živanović, L.. (2002). Statistical optimization applied to the HPLC study of methiclothiazide and amiloride in tablets. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 52(4), 470-471.
https://hdl.handle.net/21.15107/rcub_farfar_348

Zečević M, Živanović L. Statistical optimization applied to the HPLC study of methiclothiazide and amiloride in tablets. in Arhiv za farmaciju. 2002;52(4):470-471.
https://hdl.handle.net/21.15107/rcub_farfar_348 .

Zečević, Mira, Živanović, Ljiljana, "Statistical optimization applied to the HPLC study of methiclothiazide and amiloride in tablets" in Arhiv za farmaciju, 52, no. 4 (2002):470-471,
https://hdl.handle.net/21.15107/rcub_farfar_348 .

TY  - JOUR
AU  - Živanović, Ljiljana
AU  - Vojvodić, Lj
AU  - Ristić, P
AU  - Zečević, Mira
AU  - Nemcova, I
PY  - 2000
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/278
PB  - John Wiley and Sons Ltd
T2  - Biomedical Chromatography
T1  - Validation and application of the RP-HPLC method for the assay of allylestrenol and α-tocopherol in tablets
VL  - 14
IS  - 1
SP  - 56
EP  - 57
DO  - 10.1002/(SICI)1099-0801(200002)14:1 56::AID-BMC972>3.0.CO;2-Z
ER  - 

@article{
author = "Živanović, Ljiljana and Vojvodić, Lj and Ristić, P and Zečević, Mira and Nemcova, I",
year = "2000",
publisher = "John Wiley and Sons Ltd",
journal = "Biomedical Chromatography",
title = "Validation and application of the RP-HPLC method for the assay of allylestrenol and α-tocopherol in tablets",
volume = "14",
number = "1",
pages = "56-57",
doi = "10.1002/(SICI)1099-0801(200002)14:1 56::AID-BMC972>3.0.CO;2-Z"
}

Živanović, L., Vojvodić, L., Ristić, P., Zečević, M.,& Nemcova, I.. (2000). Validation and application of the RP-HPLC method for the assay of allylestrenol and α-tocopherol in tablets. in Biomedical Chromatography
John Wiley and Sons Ltd., 14(1), 56-57.
https://doi.org/10.1002/(SICI)1099-0801(200002)14:1 56::AID-BMC972>3.0.CO;2-Z

Živanović L, Vojvodić L, Ristić P, Zečević M, Nemcova I. Validation and application of the RP-HPLC method for the assay of allylestrenol and α-tocopherol in tablets. in Biomedical Chromatography. 2000;14(1):56-57.
doi:10.1002/(SICI)1099-0801(200002)14:1 56::AID-BMC972>3.0.CO;2-Z .

Živanović, Ljiljana, Vojvodić, Lj, Ristić, P, Zečević, Mira, Nemcova, I, "Validation and application of the RP-HPLC method for the assay of allylestrenol and α-tocopherol in tablets" in Biomedical Chromatography, 14, no. 1 (2000):56-57,
https://doi.org/10.1002/(SICI)1099-0801(200002)14:1 56::AID-BMC972>3.0.CO;2-Z . .

TY  - JOUR
AU  - Agatonović-Kuštrin, Snežana
AU  - Zečević, Mira
AU  - Živanović, Ljiljana
AU  - Tucker, I.G
PY  - 2000
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/279
PB  - John Wiley and Sons Ltd
T2  - Biomedical Chromatography
T1  - Structure-retention relationships of diuretics in reversed-phase liquid chromatography
VL  - 14
IS  - 1
SP  - 41
EP  - 43
DO  - 10.1002/(SICI)1099-0801(200002)14:1 41::AID-BMC964>3.0.CO;2-9
ER  - 

@article{
author = "Agatonović-Kuštrin, Snežana and Zečević, Mira and Živanović, Ljiljana and Tucker, I.G",
year = "2000",
publisher = "John Wiley and Sons Ltd",
journal = "Biomedical Chromatography",
title = "Structure-retention relationships of diuretics in reversed-phase liquid chromatography",
volume = "14",
number = "1",
pages = "41-43",
doi = "10.1002/(SICI)1099-0801(200002)14:1 41::AID-BMC964>3.0.CO;2-9"
}

Agatonović-Kuštrin, S., Zečević, M., Živanović, L.,& Tucker, I.G. (2000). Structure-retention relationships of diuretics in reversed-phase liquid chromatography. in Biomedical Chromatography
John Wiley and Sons Ltd., 14(1), 41-43.
https://doi.org/10.1002/(SICI)1099-0801(200002)14:1 41::AID-BMC964>3.0.CO;2-9

Agatonović-Kuštrin S, Zečević M, Živanović L, Tucker I. Structure-retention relationships of diuretics in reversed-phase liquid chromatography. in Biomedical Chromatography. 2000;14(1):41-43.
doi:10.1002/(SICI)1099-0801(200002)14:1 41::AID-BMC964>3.0.CO;2-9 .

Agatonović-Kuštrin, Snežana, Zečević, Mira, Živanović, Ljiljana, Tucker, I.G, "Structure-retention relationships of diuretics in reversed-phase liquid chromatography" in Biomedical Chromatography, 14, no. 1 (2000):41-43,
https://doi.org/10.1002/(SICI)1099-0801(200002)14:1 41::AID-BMC964>3.0.CO;2-9 . .

TY  - JOUR
AU  - Vujić, Zorica
AU  - Radulović, Dušanka
AU  - Živanović, Ljiljana
PY  - 1995
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/119
AB  - A sensitive spectrophotometric method for the determination of metoprolol tartrate in tablets and ampoules is presented. Using spectrophotometric measurements, it was found that metoprolol tartrate and benzyl orange form a chloroform soluble ion-pair complex with an absorption maximum at 401 nm. The composition of the ion-pair complex was determined by applying Job's method to equimolar solutions of metoprolol tartrate: benzyl orange (1:2); molar absorptivity 7.39 x 103 mol-1 cm-1. Extraction of the ion-pair complex in chloroform was accomplished easily at a Britton-Robinson's buffered optimum pH = 5.2, μ = 0.1 mol/dm3. The relative stability constant, calculated according to the method of Sommer and Job's non-equimolar solutions, was log K = 9.72 (avg. value). Beer's law was obeyed up to 3.42 μg/ml of metoprolol tartrate (the detection limit was also 3.42 μg/ml). The precision of the method was checked at three different concentrations. The RSD (n = 7) varied from 0.51 to 2.03%. Reproducibility was examined by analysing Lopresor® tablets and ampoules. Recoveries varied from 99-101%. The reported method, applied to the assay of metoprolol tartrate in tablets and ampoules, gives precise and reproducible results.
T2  - Farmaco
T1  - Spectrophotometric investigation of metopropol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms
VL  - 50
IS  - 4
SP  - 281
EP  - 284
UR  - https://hdl.handle.net/21.15107/rcub_farfar_119
ER  - 

@article{
author = "Vujić, Zorica and Radulović, Dušanka and Živanović, Ljiljana",
year = "1995",
abstract = "A sensitive spectrophotometric method for the determination of metoprolol tartrate in tablets and ampoules is presented. Using spectrophotometric measurements, it was found that metoprolol tartrate and benzyl orange form a chloroform soluble ion-pair complex with an absorption maximum at 401 nm. The composition of the ion-pair complex was determined by applying Job's method to equimolar solutions of metoprolol tartrate: benzyl orange (1:2); molar absorptivity 7.39 x 103 mol-1 cm-1. Extraction of the ion-pair complex in chloroform was accomplished easily at a Britton-Robinson's buffered optimum pH = 5.2, μ = 0.1 mol/dm3. The relative stability constant, calculated according to the method of Sommer and Job's non-equimolar solutions, was log K = 9.72 (avg. value). Beer's law was obeyed up to 3.42 μg/ml of metoprolol tartrate (the detection limit was also 3.42 μg/ml). The precision of the method was checked at three different concentrations. The RSD (n = 7) varied from 0.51 to 2.03%. Reproducibility was examined by analysing Lopresor® tablets and ampoules. Recoveries varied from 99-101%. The reported method, applied to the assay of metoprolol tartrate in tablets and ampoules, gives precise and reproducible results.",
journal = "Farmaco",
title = "Spectrophotometric investigation of metopropol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms",
volume = "50",
number = "4",
pages = "281-284",
url = "https://hdl.handle.net/21.15107/rcub_farfar_119"
}

Vujić, Z., Radulović, D.,& Živanović, L.. (1995). Spectrophotometric investigation of metopropol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms. in Farmaco, 50(4), 281-284.
https://hdl.handle.net/21.15107/rcub_farfar_119

Vujić Z, Radulović D, Živanović L. Spectrophotometric investigation of metopropol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms. in Farmaco. 1995;50(4):281-284.
https://hdl.handle.net/21.15107/rcub_farfar_119 .

Vujić, Zorica, Radulović, Dušanka, Živanović, Ljiljana, "Spectrophotometric investigation of metopropol-benzyl orange reaction and its application to the assay in pharmaceutical dosage forms" in Farmaco, 50, no. 4 (1995):281-284,
https://hdl.handle.net/21.15107/rcub_farfar_119 .