TY  - CONF
AU  - Nenić, Ivana
AU  - Baralić, Katarina
AU  - Javorac, Dragana
AU  - Buha-Đorđević, Aleksandra
AU  - Antonijević-Miljaković, Evica
AU  - Antonijević, Biljana
AU  - Bulat, Zorica
AU  - Đukić-Ćosić, Danijela
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4634
AB  - Amphetamine is a potent stimulant of central nervous system and one of the most
often abused substances. Immunochromatographic test panels for preliminary urineanalysis
are used to track its abuse. Immunochromatographic analysis is quick, simple and based on
the principle of competitive binding. Its main disadvantage is cross reactivity - tendency of
antibodies to bind structurally similar compounds, including pseudoephedrine. This study
aimed to examine the effect of pseudoephedrine-based OTC preparations on the occurrence
of false-positive results of preliminary amphetamine urineanalysis and determine the time
period during which these results may occur. The study included two subjects. TylolHot®
was used for two days, while, on the first day, single dose was administered. On the second
day, preparation was administered according to the dosing regimen recommended by the
manufacturer (three times a day). Urine samples were collected for three consecutive days.
Analysis was performed by immunochromatographic test strips "DIAQUICK" Multi-Drug Test
Panels. TylolHot® use led to false positive results for amphetamine 3.75 h after single
administration, and lasted for 27 h with repeated administration. After the application of the
tested preparation, false positive results on amphetamine were obtained in different time
periods, which depended on the intra- and interindividual variations, as well as the dosing
regimen. Although test strips are used for quick preliminary analysis, it is necessary to check
the results via confirmation methods (gas or liquid chromatography coupled to mass
spectrometry). The obtained results are significant during routine testing of patients,
employees, students, athletes and members of the army.
AB  - Amfetamin je snažan stimulans centralnog nervnog sistema i jedna od supstanci koja
se najčešće zloupotrebljavaju. Za praćenje njegove zloupotrebe koriste se
imunohromatografske test trake za preliminarnu analizu urina. Imunohromatografska
analiza je brza i jednostavna metoda koja se zasniva na principu kompetitivnog vezivanja.
Njen glavni nedostatak je unakrsna reaktivnost – sklonost antitela da vezuju strukturno
slična jedinjenja, između ostalih i pseudoefedrin. Cilj ovog rada bio je ispitati uticaj primene
OTC preparata na bazi pseudoefedrina na pojavu lažno pozitivnih rezultata preliminarne
analize amfetamina u urinu i utvrditi vremenski period pojave ovih rezultata od trenutka
primene preparata. Ispitivanje je uključivalo dva ispitanika. Tokom dva dana korišćen je
preparat TylolHot® , pri čemu je prvi dan primenjena jedna doza, a drugi dan se preparat
primenjivao prema režimu doziranja preporučenom od strane proizvođača (tri puta
dnevno). Uzorci urina sakupljani su tokom tri uzastopna dana, a analiza je izvršena
imunohromatografskim test trakama "DIAQUICK" Multi-Drug Test Panels. Dobijeni su lažno
pozitivni rezultati na amfetamin već u periodu od 3,75 h nakon jednokratne primene
TylolHot ® preparata, i trajali su tokom 27 h pri višekratnoj primeni. Utvrđeno je da se nakon
primene TylolHot® preparata u različitim vremenskim periodima mogu dobiti lažno pozitivni
rezultati, što zavisi od intra- i interindividualnih varijacija i režima doziranja. Iako se test
trake koriste za brzu preliminarnu analizu, neophodno je potvrditi rezultate konfirmativnim
metodama (gasna ili tečna hromatografija spregnuta sa masenom spektrometrijom).
Dobijeni rezultati su značajni prilikom rutinskih testiranja pacijenata, zaposlenih, učenika,
sportista i pripadnika vojske.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Time-dependent occurrence of false positive amphetamine test strips results after consumption of OTC preparations with pseudoephedrine
T1  - Vremenski zavisna pojava lažno pozitivnih rezultata test traka na amfetamin nakon primene OTC preparata sa pseudoefedrinom
VL  - 72
IS  - 4 suplement
SP  - S595
EP  - S595
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4634
ER  - 

@conference{
author = "Nenić, Ivana and Baralić, Katarina and Javorac, Dragana and Buha-Đorđević, Aleksandra and Antonijević-Miljaković, Evica and Antonijević, Biljana and Bulat, Zorica and Đukić-Ćosić, Danijela",
year = "2022",
abstract = "Amphetamine is a potent stimulant of central nervous system and one of the most
often abused substances. Immunochromatographic test panels for preliminary urineanalysis
are used to track its abuse. Immunochromatographic analysis is quick, simple and based on
the principle of competitive binding. Its main disadvantage is cross reactivity - tendency of
antibodies to bind structurally similar compounds, including pseudoephedrine. This study
aimed to examine the effect of pseudoephedrine-based OTC preparations on the occurrence
of false-positive results of preliminary amphetamine urineanalysis and determine the time
period during which these results may occur. The study included two subjects. TylolHot®
was used for two days, while, on the first day, single dose was administered. On the second
day, preparation was administered according to the dosing regimen recommended by the
manufacturer (three times a day). Urine samples were collected for three consecutive days.
Analysis was performed by immunochromatographic test strips "DIAQUICK" Multi-Drug Test
Panels. TylolHot® use led to false positive results for amphetamine 3.75 h after single
administration, and lasted for 27 h with repeated administration. After the application of the
tested preparation, false positive results on amphetamine were obtained in different time
periods, which depended on the intra- and interindividual variations, as well as the dosing
regimen. Although test strips are used for quick preliminary analysis, it is necessary to check
the results via confirmation methods (gas or liquid chromatography coupled to mass
spectrometry). The obtained results are significant during routine testing of patients,
employees, students, athletes and members of the army., Amfetamin je snažan stimulans centralnog nervnog sistema i jedna od supstanci koja
se najčešće zloupotrebljavaju. Za praćenje njegove zloupotrebe koriste se
imunohromatografske test trake za preliminarnu analizu urina. Imunohromatografska
analiza je brza i jednostavna metoda koja se zasniva na principu kompetitivnog vezivanja.
Njen glavni nedostatak je unakrsna reaktivnost – sklonost antitela da vezuju strukturno
slična jedinjenja, između ostalih i pseudoefedrin. Cilj ovog rada bio je ispitati uticaj primene
OTC preparata na bazi pseudoefedrina na pojavu lažno pozitivnih rezultata preliminarne
analize amfetamina u urinu i utvrditi vremenski period pojave ovih rezultata od trenutka
primene preparata. Ispitivanje je uključivalo dva ispitanika. Tokom dva dana korišćen je
preparat TylolHot® , pri čemu je prvi dan primenjena jedna doza, a drugi dan se preparat
primenjivao prema režimu doziranja preporučenom od strane proizvođača (tri puta
dnevno). Uzorci urina sakupljani su tokom tri uzastopna dana, a analiza je izvršena
imunohromatografskim test trakama "DIAQUICK" Multi-Drug Test Panels. Dobijeni su lažno
pozitivni rezultati na amfetamin već u periodu od 3,75 h nakon jednokratne primene
TylolHot ® preparata, i trajali su tokom 27 h pri višekratnoj primeni. Utvrđeno je da se nakon
primene TylolHot® preparata u različitim vremenskim periodima mogu dobiti lažno pozitivni
rezultati, što zavisi od intra- i interindividualnih varijacija i režima doziranja. Iako se test
trake koriste za brzu preliminarnu analizu, neophodno je potvrditi rezultate konfirmativnim
metodama (gasna ili tečna hromatografija spregnuta sa masenom spektrometrijom).
Dobijeni rezultati su značajni prilikom rutinskih testiranja pacijenata, zaposlenih, učenika,
sportista i pripadnika vojske.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Time-dependent occurrence of false positive amphetamine test strips results after consumption of OTC preparations with pseudoephedrine, Vremenski zavisna pojava lažno pozitivnih rezultata test traka na amfetamin nakon primene OTC preparata sa pseudoefedrinom",
volume = "72",
number = "4 suplement",
pages = "S595-S595",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4634"
}

Nenić, I., Baralić, K., Javorac, D., Buha-Đorđević, A., Antonijević-Miljaković, E., Antonijević, B., Bulat, Z.,& Đukić-Ćosić, D.. (2022). Time-dependent occurrence of false positive amphetamine test strips results after consumption of OTC preparations with pseudoephedrine. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S595-S595.
https://hdl.handle.net/21.15107/rcub_farfar_4634

Nenić I, Baralić K, Javorac D, Buha-Đorđević A, Antonijević-Miljaković E, Antonijević B, Bulat Z, Đukić-Ćosić D. Time-dependent occurrence of false positive amphetamine test strips results after consumption of OTC preparations with pseudoephedrine. in Arhiv za farmaciju. 2022;72(4 suplement):S595-S595.
https://hdl.handle.net/21.15107/rcub_farfar_4634 .

Nenić, Ivana, Baralić, Katarina, Javorac, Dragana, Buha-Đorđević, Aleksandra, Antonijević-Miljaković, Evica, Antonijević, Biljana, Bulat, Zorica, Đukić-Ćosić, Danijela, "Time-dependent occurrence of false positive amphetamine test strips results after consumption of OTC preparations with pseudoephedrine" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S595-S595,
https://hdl.handle.net/21.15107/rcub_farfar_4634 .