@conference{
author = "Stojilković, Nikola and Stojković, Miodrag and Ćurčić, Marijana and Javorac, Dragana and Buha-Đorđević, Aleksandra and Stojanović, Aleksandra and Zdravković, Aleksandra and Stojadinović, Tina and Marić, Đurđica and Baralić, Katarina and Antonijević, Biljana",
year = "2022",
abstract = "Benchmark dose (BMD) approach extends the use of data and allows a quantification
of the uncertainties and confirmation of the effect. The objective of this study was to
investigate whether a mixture of lead (Pb) and polychlorinated-biphenyls (PCBs)
has any effect on sperm cells, and whether these effects are dose dependent. The
experiment was conducted in accordance with the approval of Ethical Committee No. 323-
07-11822/2018-05. Male Wistar rats were exposed to the mixture of Pb and PCBs by oral
gavage for 28 days. Doses od Pb in mixture were 0.1 mg/kg/day, 0.5 mg/kg/day and 1
mg/kg/day, while doses of PCBs in mixture were 0.25 mg/kg/day, 0.5 mg/kg/day, and 1
mg/kg/day respectively. Rats were anesthetized by the combination of ketamine/xylazine.
Through the scrotum section, the right testis and epididymis were collected, while sperm
cells were taken from the cauda of epididymis by diffusion in saline solution at 37°C. Sperm
motililty was determined by the light microscope. The aliquot of the sperm cells was fixated
with the combination of formalin in the phosphate buffer and used for morphology and
concentration sperm analysis. The benchmark dose lower confidence limit (BMDL40) of
0.00013 mg/kg/day was derived for the sperm motility effect of Pb and PCBs as co-variant.
From our results, we can conclude that the mortality was the most sensitive parameter. This
value is also one of the lowest BMDL for Pb, which can lead to conclusion that PCBs increase
the potency of Pb toxicity., Pristup računanja Benchmark doze (BMD) proširuje upotrebu podataka, omogućava
kvantifikaciju nesigurnosti i potvrđuje efekat. Cilj ove studije bio je da se ispita da li smeša
olova (Pb) i polihlorovanih-bifenila (PCBs) ispoljava efekat na ćelije sperme, kao i da se ispita
da li su efekti dozno zavisni. Eksperiment je sproveden u skladu sa odobrenjem Etičkog
komiteta br. 323-07-11822 / 2018-05. Mužjaci Wistar pacova su bili izloženi smeši Pb i PCBs
oralnom gavažom tokom 28 dana. Doze Pb u smeši bile su 0,1 mg/kg/dan, 0,5 mg/kg/dan i 1
mg/kg/dan, dok su doze PCBs u smeši bile 0,25 mg/kg/dan, 0,5 mg/kg/dan i 1 mg/kg/dan.
Pacovi su anestezirani kombinacijom ketamin/ksilazin. Kroz presek skrotuma, sakupljeni su
desni testis i epididimis, spermatozoidi su prikupljeni u fiziološkom rastvoru na 37°C
difuzijom iz repnog dela epididimisa. Pokretljivost spermatozoida je određena svetlosnim
mikroskopom. Deo rastvora spermatozoida je fiksiran kombinacijom formalina u fosfatnom
puferu i korišćen za analizu morfologije i koncentracije spermatozoida. Donja granica
pouzdanosti benčmark doze (BMDL40) od 0,00013 mg/kg/dan je izračunata za pokretljivost
spermatozoida za Pb i PCBs-ima kao kovarijantu. Iz naših rezultata možemo zaključiti da je
najosetljiviji parametar pokretljivost spermatozoida. Ova vrednost je takođe jedna od
najnižih BMDL za Pb, što može dovesti do zaključka da PCBs povećavaju toksičnosti olova
kada su u smeši.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Effect of Lead and Polychlorinated-biphenyls Mixture on Sperm Cells in Rats, Efekti olova i polihlorovanih ‐ bifenila na ćelije sperme kod pacova",
volume = "72",
number = "4 suplement",
pages = "S583-S584",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4631"
}
