TY  - JOUR
AU  - Radović, Biljana
AU  - Baralić, Katarina
AU  - Ćurčić, Marijana
AU  - Marić, Đurđica
AU  - Živanović, Jovana
AU  - Antonijević-Miljaković, Evica
AU  - Buha-Đorđević, Aleksandra
AU  - Đukić-Ćosić, Danijela
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5530
AB  - The constant exposure of humans to a mixture of low doses of toxic substances, emerging from the daily emission of toxic dust containing various metals and organic compounds in electrical and electronic waste (e-waste) recycling areas, poses potential harmful effects on health and the environment. While individually recognized as endocrine disruptors affecting hormonal balance, the combined impact of these toxic substances in a mixture remains insufficiently explored, particularly in relation to reproductive health. Thus, the aim of this in silico analysis was to: (i) assess the relationship between the exposure to a mixture of DBDE, DBDPE, TBBPA, Pb, Cd and Ni and development of male and female reproductive system disorders; and (ii) demonstrate the ability of in silico toxicogenomic tools in revealing the potential molecular mechanisms involved in the mixture toxicity. As the main data-mining tool, Comparative Toxicogenomics Database (CTD) was used, along with the ToppGene Suite portal and GeneMANIA online server. Our analysis identified 5 genes common to all the investigated substances and linked to reproductive system disorders. Notably, the most prominent interactions among these genes were physical interactions (77.64 %). Pathway enrichment analysis identified oxidative stress response as the central disrupted molecular pathway linked to reproductive pathology in the investigated mixture, while our chemical-phenotype CTD analysis uncovered additional affected pathways – apoptosis, hormonal regulation, and developmental functions. These findings highlight an increased risk of reproductive system disorders associated with the exposure to the investigated mixture of toxic substances in electronic waste recycling areas, emphasizing the urgent need for attention to address this environmental health concern. Hence, future laboratory studies should prioritize investigating the specific genes and common mechanisms identified in this study.
PB  - Elsevier V. B.
T2  - Science of the Total Environment
T1  - Endocrine disruptors in e-waste dismantling dust: In silico prediction of mixture-induced reproductive toxicity mechanisms
VL  - 917
SP  - 170437
DO  - 10.1016/j.scitotenv.2024.170437
ER  - 

@article{
author = "Radović, Biljana and Baralić, Katarina and Ćurčić, Marijana and Marić, Đurđica and Živanović, Jovana and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Đukić-Ćosić, Danijela and Bulat, Zorica and Antonijević, Biljana",
year = "2024",
abstract = "The constant exposure of humans to a mixture of low doses of toxic substances, emerging from the daily emission of toxic dust containing various metals and organic compounds in electrical and electronic waste (e-waste) recycling areas, poses potential harmful effects on health and the environment. While individually recognized as endocrine disruptors affecting hormonal balance, the combined impact of these toxic substances in a mixture remains insufficiently explored, particularly in relation to reproductive health. Thus, the aim of this in silico analysis was to: (i) assess the relationship between the exposure to a mixture of DBDE, DBDPE, TBBPA, Pb, Cd and Ni and development of male and female reproductive system disorders; and (ii) demonstrate the ability of in silico toxicogenomic tools in revealing the potential molecular mechanisms involved in the mixture toxicity. As the main data-mining tool, Comparative Toxicogenomics Database (CTD) was used, along with the ToppGene Suite portal and GeneMANIA online server. Our analysis identified 5 genes common to all the investigated substances and linked to reproductive system disorders. Notably, the most prominent interactions among these genes were physical interactions (77.64 %). Pathway enrichment analysis identified oxidative stress response as the central disrupted molecular pathway linked to reproductive pathology in the investigated mixture, while our chemical-phenotype CTD analysis uncovered additional affected pathways – apoptosis, hormonal regulation, and developmental functions. These findings highlight an increased risk of reproductive system disorders associated with the exposure to the investigated mixture of toxic substances in electronic waste recycling areas, emphasizing the urgent need for attention to address this environmental health concern. Hence, future laboratory studies should prioritize investigating the specific genes and common mechanisms identified in this study.",
publisher = "Elsevier V. B.",
journal = "Science of the Total Environment",
title = "Endocrine disruptors in e-waste dismantling dust: In silico prediction of mixture-induced reproductive toxicity mechanisms",
volume = "917",
pages = "170437",
doi = "10.1016/j.scitotenv.2024.170437"
}

Radović, B., Baralić, K., Ćurčić, M., Marić, Đ., Živanović, J., Antonijević-Miljaković, E., Buha-Đorđević, A., Đukić-Ćosić, D., Bulat, Z.,& Antonijević, B.. (2024). Endocrine disruptors in e-waste dismantling dust: In silico prediction of mixture-induced reproductive toxicity mechanisms. in Science of the Total Environment
Elsevier V. B.., 917, 170437.
https://doi.org/10.1016/j.scitotenv.2024.170437

Radović B, Baralić K, Ćurčić M, Marić Đ, Živanović J, Antonijević-Miljaković E, Buha-Đorđević A, Đukić-Ćosić D, Bulat Z, Antonijević B. Endocrine disruptors in e-waste dismantling dust: In silico prediction of mixture-induced reproductive toxicity mechanisms. in Science of the Total Environment. 2024;917:170437.
doi:10.1016/j.scitotenv.2024.170437 .

Radović, Biljana, Baralić, Katarina, Ćurčić, Marijana, Marić, Đurđica, Živanović, Jovana, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Đukić-Ćosić, Danijela, Bulat, Zorica, Antonijević, Biljana, "Endocrine disruptors in e-waste dismantling dust: In silico prediction of mixture-induced reproductive toxicity mechanisms" in Science of the Total Environment, 917 (2024):170437,
https://doi.org/10.1016/j.scitotenv.2024.170437 . .

TY  - CONF
AU  - Radović, Biljana
AU  - Stojilković, Nikola
AU  - Ćurčić, Marijana
AU  - Antonijević-Miljaković, Evica
AU  - Buha-Đorđević, Aleksandra
AU  - Vukelić, Dragana
AU  - Baralić, Katarina
AU  - Đukić-Ćosić, Danijela
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5485
AB  - Aktivnosti prilikom rastavljanje e-otpada predstavljaju značajan izvor mnogih štetnih komponenti, što dovodi do svakodnevnog izlaganja radnika toksičnim isparenjima koja se sastoje od različitih metala i organskih jedinjenja. Svaka od ovih hemikalija iz životne sredine ponaosob ispoljava toksične efekte ometajući homeostazu hormona, a njihov potencijal da deluju kao endokrini ometači je već potvrđen. ...
AB  - E-waste dismantling activities constitute a significant source of many harmful components, resulting in everyday exposure of workers to toxic fumes consisting of different metals and organic compounds. Each of these environmental chemicals individually exerts toxic effects by interfering with hormone homeostasis and their potential to act as endocrine disruptors (EDs) has already been confirmed. ...
PB  - Udruženje toksikologa Srbije
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book, 2023, 57-58
T1  - Prašina od rastavljanja e-otpada kao izvor hemikalija koje ometaju rad endokrinog sistema: In silico testiranje toksičnosti smeše na štitnu žlezdu i muški reproduktivni sistem
T1  - Dust from e - waste dismantling as a source of endocrine disrupting chemicals: In silico testing of mixture toxicity on thyroid gland and male reproductive system
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5485
ER  - 

@conference{
author = "Radović, Biljana and Stojilković, Nikola and Ćurčić, Marijana and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Vukelić, Dragana and Baralić, Katarina and Đukić-Ćosić, Danijela and Bulat, Zorica and Antonijević, Biljana",
year = "2023",
abstract = "Aktivnosti prilikom rastavljanje e-otpada predstavljaju značajan izvor mnogih štetnih komponenti, što dovodi do svakodnevnog izlaganja radnika toksičnim isparenjima koja se sastoje od različitih metala i organskih jedinjenja. Svaka od ovih hemikalija iz životne sredine ponaosob ispoljava toksične efekte ometajući homeostazu hormona, a njihov potencijal da deluju kao endokrini ometači je već potvrđen. ..., E-waste dismantling activities constitute a significant source of many harmful components, resulting in everyday exposure of workers to toxic fumes consisting of different metals and organic compounds. Each of these environmental chemicals individually exerts toxic effects by interfering with hormone homeostasis and their potential to act as endocrine disruptors (EDs) has already been confirmed. ...",
publisher = "Udruženje toksikologa Srbije",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book, 2023, 57-58",
title = "Prašina od rastavljanja e-otpada kao izvor hemikalija koje ometaju rad endokrinog sistema: In silico testiranje toksičnosti smeše na štitnu žlezdu i muški reproduktivni sistem, Dust from e - waste dismantling as a source of endocrine disrupting chemicals: In silico testing of mixture toxicity on thyroid gland and male reproductive system",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5485"
}

Radović, B., Stojilković, N., Ćurčić, M., Antonijević-Miljaković, E., Buha-Đorđević, A., Vukelić, D., Baralić, K., Đukić-Ćosić, D., Bulat, Z.,& Antonijević, B.. (2023). Prašina od rastavljanja e-otpada kao izvor hemikalija koje ometaju rad endokrinog sistema: In silico testiranje toksičnosti smeše na štitnu žlezdu i muški reproduktivni sistem. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book, 2023, 57-58
Udruženje toksikologa Srbije..
https://hdl.handle.net/21.15107/rcub_farfar_5485

Radović B, Stojilković N, Ćurčić M, Antonijević-Miljaković E, Buha-Đorđević A, Vukelić D, Baralić K, Đukić-Ćosić D, Bulat Z, Antonijević B. Prašina od rastavljanja e-otpada kao izvor hemikalija koje ometaju rad endokrinog sistema: In silico testiranje toksičnosti smeše na štitnu žlezdu i muški reproduktivni sistem. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book, 2023, 57-58. 2023;.
https://hdl.handle.net/21.15107/rcub_farfar_5485 .

Radović, Biljana, Stojilković, Nikola, Ćurčić, Marijana, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Vukelić, Dragana, Baralić, Katarina, Đukić-Ćosić, Danijela, Bulat, Zorica, Antonijević, Biljana, "Prašina od rastavljanja e-otpada kao izvor hemikalija koje ometaju rad endokrinog sistema: In silico testiranje toksičnosti smeše na štitnu žlezdu i muški reproduktivni sistem" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book, 2023, 57-58 (2023),
https://hdl.handle.net/21.15107/rcub_farfar_5485 .

TY  - CONF
AU  - Radović, Biljana
AU  - Stojilković, Nikola
AU  - Ćurčić, Marijana
AU  - Antonijević-Miljaković, Evica
AU  - Buha-Đorđević, Aleksandra
AU  - Vukelić, Dragana
AU  - Baralić, Katarina
AU  - Marić, Đurđica
AU  - Đukić-Ćosić, Danijela
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5429
AB  - Sobzirom na to da su ljudi u svakodnevnom životu konstantno izloženi smešama hemikalija koje deluju
putem zajedničkih mehanizama, važno je proceniti da li su ovi „koktel efekti“ izraženiji u poređenju sa
dejstvom pojedinačnih hemikalija. ...
AB  - Due to the fact that in daily life humans are constantly exposed to mixtures of chemicals acting by
a common mechanism of action, it is important to assess whether these "cocktail effects" are more
pronounced compared to the effects of single chemicals. ...
PB  - Udruženje toksikologa Srbije
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book
T1  - Procena toksičnih efekata smeše olova i polihlorovanih bifenila na redoks status u bubrezima pacova
T1  - Assessment of lead and polychlorinated biphenyls mixture toxic effects on redox status in rat kidneys
SP  - 67
EP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5429
ER  - 

@conference{
author = "Radović, Biljana and Stojilković, Nikola and Ćurčić, Marijana and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Vukelić, Dragana and Baralić, Katarina and Marić, Đurđica and Đukić-Ćosić, Danijela and Bulat, Zorica and Antonijević, Biljana",
year = "2023",
abstract = "Sobzirom na to da su ljudi u svakodnevnom životu konstantno izloženi smešama hemikalija koje deluju
putem zajedničkih mehanizama, važno je proceniti da li su ovi „koktel efekti“ izraženiji u poređenju sa
dejstvom pojedinačnih hemikalija. ..., Due to the fact that in daily life humans are constantly exposed to mixtures of chemicals acting by
a common mechanism of action, it is important to assess whether these "cocktail effects" are more
pronounced compared to the effects of single chemicals. ...",
publisher = "Udruženje toksikologa Srbije",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book",
title = "Procena toksičnih efekata smeše olova i polihlorovanih bifenila na redoks status u bubrezima pacova, Assessment of lead and polychlorinated biphenyls mixture toxic effects on redox status in rat kidneys",
pages = "67-68",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5429"
}

Radović, B., Stojilković, N., Ćurčić, M., Antonijević-Miljaković, E., Buha-Đorđević, A., Vukelić, D., Baralić, K., Marić, Đ., Đukić-Ćosić, D., Bulat, Z.,& Antonijević, B.. (2023). Procena toksičnih efekata smeše olova i polihlorovanih bifenila na redoks status u bubrezima pacova. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book
Udruženje toksikologa Srbije., 67-68.
https://hdl.handle.net/21.15107/rcub_farfar_5429

Radović B, Stojilković N, Ćurčić M, Antonijević-Miljaković E, Buha-Đorđević A, Vukelić D, Baralić K, Marić Đ, Đukić-Ćosić D, Bulat Z, Antonijević B. Procena toksičnih efekata smeše olova i polihlorovanih bifenila na redoks status u bubrezima pacova. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book. 2023;:67-68.
https://hdl.handle.net/21.15107/rcub_farfar_5429 .

Radović, Biljana, Stojilković, Nikola, Ćurčić, Marijana, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Vukelić, Dragana, Baralić, Katarina, Marić, Đurđica, Đukić-Ćosić, Danijela, Bulat, Zorica, Antonijević, Biljana, "Procena toksičnih efekata smeše olova i polihlorovanih bifenila na redoks status u bubrezima pacova" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference, 10-12 May, 2023, Belgrade, Abstract Book (2023):67-68,
https://hdl.handle.net/21.15107/rcub_farfar_5429 .

TY  - CONF
AU  - Radović, Biljana
AU  - Stojilković, Nikola
AU  - Ćurčić, Marijana
AU  - Antonijević-Miljaković, Evica
AU  - Buha-Đorđević, Aleksandra
AU  - Baralić, Katarina
AU  - Vukelić, Dragana
AU  - Đukić-Ćosić, Danijela
AU  - Marić, Đurđica
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5175
AB  - Considering that combined exposure to toxic metals and organic pollutants may induce various adverse effects on human health, it is important to elucidate the mechanisms of toxicity that occur after lowdose exposure to these chemicals in a real-life scenario. ...
PB  - Elsevier
C3  - Toxicology Letters
T1  - Oxidative damage in liver tissue induced by low doses exposure to a mixture of lead and polychlorinated biphenyls: Subacute toxicity study in rats
VL  - 384
IS  - S1
SP  - S223
EP  - S223
DO  - 10.1016/S0378-4274(23)00790-7
ER  - 

@conference{
author = "Radović, Biljana and Stojilković, Nikola and Ćurčić, Marijana and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Baralić, Katarina and Vukelić, Dragana and Đukić-Ćosić, Danijela and Marić, Đurđica and Bulat, Zorica and Antonijević, Biljana",
year = "2023",
abstract = "Considering that combined exposure to toxic metals and organic pollutants may induce various adverse effects on human health, it is important to elucidate the mechanisms of toxicity that occur after lowdose exposure to these chemicals in a real-life scenario. ...",
publisher = "Elsevier",
journal = "Toxicology Letters",
title = "Oxidative damage in liver tissue induced by low doses exposure to a mixture of lead and polychlorinated biphenyls: Subacute toxicity study in rats",
volume = "384",
number = "S1",
pages = "S223-S223",
doi = "10.1016/S0378-4274(23)00790-7"
}

Radović, B., Stojilković, N., Ćurčić, M., Antonijević-Miljaković, E., Buha-Đorđević, A., Baralić, K., Vukelić, D., Đukić-Ćosić, D., Marić, Đ., Bulat, Z.,& Antonijević, B.. (2023). Oxidative damage in liver tissue induced by low doses exposure to a mixture of lead and polychlorinated biphenyls: Subacute toxicity study in rats. in Toxicology Letters
Elsevier., 384(S1), S223-S223.
https://doi.org/10.1016/S0378-4274(23)00790-7

Radović B, Stojilković N, Ćurčić M, Antonijević-Miljaković E, Buha-Đorđević A, Baralić K, Vukelić D, Đukić-Ćosić D, Marić Đ, Bulat Z, Antonijević B. Oxidative damage in liver tissue induced by low doses exposure to a mixture of lead and polychlorinated biphenyls: Subacute toxicity study in rats. in Toxicology Letters. 2023;384(S1):S223-S223.
doi:10.1016/S0378-4274(23)00790-7 .

Radović, Biljana, Stojilković, Nikola, Ćurčić, Marijana, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Baralić, Katarina, Vukelić, Dragana, Đukić-Ćosić, Danijela, Marić, Đurđica, Bulat, Zorica, Antonijević, Biljana, "Oxidative damage in liver tissue induced by low doses exposure to a mixture of lead and polychlorinated biphenyls: Subacute toxicity study in rats" in Toxicology Letters, 384, no. S1 (2023):S223-S223,
https://doi.org/10.1016/S0378-4274(23)00790-7 . .

TY  - JOUR
AU  - Stojilković, Nikola
AU  - Radović, Biljana
AU  - Vukelić, Dragana
AU  - Ćurčić, Marijana
AU  - Antonijević-Miljaković, Evica
AU  - Buha-Đorđević, Aleksandra
AU  - Baralić, Katarina
AU  - Marić, Đurđica
AU  - Bulat, Zorica
AU  - Đukić-Ćosić, Danijela
AU  - Antonijević, Biljana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5142
AB  - Toxicological research is mostly limited to considering the effects of a single substance, even though the real exposure of people is reflected in their daily exposure to many different chemical substances in low-doses. This in silico toxicogenomic study aims to provide evidence for the selected environmental (organo)metals (lead, cadmium, methyl mercury) + polychlorinated biphenyls mixture involvement in the possible alteration of thyroid, and male reproductive system function, and furthermore to predict the possible toxic mechanisms of the environmental cocktail. The Comparative Toxicogenomic Database, GeneMANIA online software, and ToppGene Suite portal were used as the main tools for toxicogenomic data mining and gene ontology analysis. The results show that 35 annotated common genes between selected chemicals and endocrine system diseases can interact on the co-expression level. Our study highlighted the disruption of the cytokines, the cell's response to oxidative stress, and the influence of the transcription factors as the potential core of toxicological mechanisms of the discussed mixture's effects. The connected toxicological effects of the tested mixture were abnormal sperm cells, a disrupted level of testosterone, and thyroid hormones. The core mechanisms of these effects were inflammation, oxidative stress, disruption of androgen receptor signaling, and the alteration of the FOXO3-Keap-1/NRF2-HMOX1-NQO1 pathway signaling most likely controlled by the co-expression of overlapped genes among used chemicals. This in silico research can be used as a potential core for the determination of biomarkers that can be monitored in future further in vitro and in vivo experiments.
PB  - Elsevier
T2  - Environmental Research
T1  - Involvement of toxic metals and PCBs mixture in the thyroid and male reproductive toxicity: In silico toxicogenomic data mining
DO  - 10.1016/j.envres.2023.117274
ER  - 

@article{
author = "Stojilković, Nikola and Radović, Biljana and Vukelić, Dragana and Ćurčić, Marijana and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Baralić, Katarina and Marić, Đurđica and Bulat, Zorica and Đukić-Ćosić, Danijela and Antonijević, Biljana",
year = "2023",
abstract = "Toxicological research is mostly limited to considering the effects of a single substance, even though the real exposure of people is reflected in their daily exposure to many different chemical substances in low-doses. This in silico toxicogenomic study aims to provide evidence for the selected environmental (organo)metals (lead, cadmium, methyl mercury) + polychlorinated biphenyls mixture involvement in the possible alteration of thyroid, and male reproductive system function, and furthermore to predict the possible toxic mechanisms of the environmental cocktail. The Comparative Toxicogenomic Database, GeneMANIA online software, and ToppGene Suite portal were used as the main tools for toxicogenomic data mining and gene ontology analysis. The results show that 35 annotated common genes between selected chemicals and endocrine system diseases can interact on the co-expression level. Our study highlighted the disruption of the cytokines, the cell's response to oxidative stress, and the influence of the transcription factors as the potential core of toxicological mechanisms of the discussed mixture's effects. The connected toxicological effects of the tested mixture were abnormal sperm cells, a disrupted level of testosterone, and thyroid hormones. The core mechanisms of these effects were inflammation, oxidative stress, disruption of androgen receptor signaling, and the alteration of the FOXO3-Keap-1/NRF2-HMOX1-NQO1 pathway signaling most likely controlled by the co-expression of overlapped genes among used chemicals. This in silico research can be used as a potential core for the determination of biomarkers that can be monitored in future further in vitro and in vivo experiments.",
publisher = "Elsevier",
journal = "Environmental Research",
title = "Involvement of toxic metals and PCBs mixture in the thyroid and male reproductive toxicity: In silico toxicogenomic data mining",
doi = "10.1016/j.envres.2023.117274"
}

Stojilković, N., Radović, B., Vukelić, D., Ćurčić, M., Antonijević-Miljaković, E., Buha-Đorđević, A., Baralić, K., Marić, Đ., Bulat, Z., Đukić-Ćosić, D.,& Antonijević, B.. (2023). Involvement of toxic metals and PCBs mixture in the thyroid and male reproductive toxicity: In silico toxicogenomic data mining. in Environmental Research
Elsevier..
https://doi.org/10.1016/j.envres.2023.117274

Stojilković N, Radović B, Vukelić D, Ćurčić M, Antonijević-Miljaković E, Buha-Đorđević A, Baralić K, Marić Đ, Bulat Z, Đukić-Ćosić D, Antonijević B. Involvement of toxic metals and PCBs mixture in the thyroid and male reproductive toxicity: In silico toxicogenomic data mining. in Environmental Research. 2023;.
doi:10.1016/j.envres.2023.117274 .

Stojilković, Nikola, Radović, Biljana, Vukelić, Dragana, Ćurčić, Marijana, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Baralić, Katarina, Marić, Đurđica, Bulat, Zorica, Đukić-Ćosić, Danijela, Antonijević, Biljana, "Involvement of toxic metals and PCBs mixture in the thyroid and male reproductive toxicity: In silico toxicogenomic data mining" in Environmental Research (2023),
https://doi.org/10.1016/j.envres.2023.117274 . .

TY  - JOUR
AU  - Radović, Biljana
AU  - Stojilković, Nikola
AU  - Ćurčić, Marijana
AU  - Antonijević-Miljaković, Evica
AU  - Buha-Đorđević, Aleksandra
AU  - Vukelić-Javorac, Dragana
AU  - Baralić, Katarina
AU  - Đukić-Ćosić, Danijela
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4613
AB  - The current study aimed to assess the connection between the mixture of lead (Pb), cadmium (Cd), arsenic (As), methylmercury (MeHg) and decabrominated diphenyl ether (decaBDE) and thyroid function, by using in silico toxicogenomic data-mining approach. To obtain the linkage between investigated toxic mixture and thyroid diseases (TDs), the Comparative Toxicogenomics Database (CTD) was used, while gene ontology (GO) enrichment analysis was performed by ToppGeneSuite portal. The analysis has shown 10 genes connected to all chemicals present in the mixture and TDs (CAT, GSR, IFNG, IL1B, IL4, IL6, MAPK1, SOD2, TGFB1, TNF), most of which were in co-expression (45.68%), or belonged to the same pathway (30.47%). Top 5 biological processes and molecular functions affected by the investigated mixture emphasized the role of two common mechanisms – oxidative stress and inflammation. Cytokines and inflammatory response was listed as the main molecular pathway that may be triggered by simultaneous exposure to toxic metal(oid)s and decaBDE and connected to TDs. The direct relations between Pb/decaBDE and redox status impairment in thyroid tissue was confirmed by our chemical-phenotype interaction analysis, while the strongest linkage between Pb, As and decaBDE and thyroid disorders was found. The obtained results provide better understanding of molecular mechanisms involved in the thyrotoxicity of the investigated mixture, and can be used to direct further research.
PB  - Elsevier Ireland Ltd
T2  - Toxicology
T1  - In silico assessment of mixture toxicity mechanisms involved in the pathogenesis of thyroid diseases: The combination of toxic metal(oid)s and decabrominated diphenyl ether
VL  - 489
DO  - 10.1016/j.tox.2023.153496
ER  - 

@article{
author = "Radović, Biljana and Stojilković, Nikola and Ćurčić, Marijana and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Vukelić-Javorac, Dragana and Baralić, Katarina and Đukić-Ćosić, Danijela and Bulat, Zorica and Antonijević, Biljana",
year = "2023",
abstract = "The current study aimed to assess the connection between the mixture of lead (Pb), cadmium (Cd), arsenic (As), methylmercury (MeHg) and decabrominated diphenyl ether (decaBDE) and thyroid function, by using in silico toxicogenomic data-mining approach. To obtain the linkage between investigated toxic mixture and thyroid diseases (TDs), the Comparative Toxicogenomics Database (CTD) was used, while gene ontology (GO) enrichment analysis was performed by ToppGeneSuite portal. The analysis has shown 10 genes connected to all chemicals present in the mixture and TDs (CAT, GSR, IFNG, IL1B, IL4, IL6, MAPK1, SOD2, TGFB1, TNF), most of which were in co-expression (45.68%), or belonged to the same pathway (30.47%). Top 5 biological processes and molecular functions affected by the investigated mixture emphasized the role of two common mechanisms – oxidative stress and inflammation. Cytokines and inflammatory response was listed as the main molecular pathway that may be triggered by simultaneous exposure to toxic metal(oid)s and decaBDE and connected to TDs. The direct relations between Pb/decaBDE and redox status impairment in thyroid tissue was confirmed by our chemical-phenotype interaction analysis, while the strongest linkage between Pb, As and decaBDE and thyroid disorders was found. The obtained results provide better understanding of molecular mechanisms involved in the thyrotoxicity of the investigated mixture, and can be used to direct further research.",
publisher = "Elsevier Ireland Ltd",
journal = "Toxicology",
title = "In silico assessment of mixture toxicity mechanisms involved in the pathogenesis of thyroid diseases: The combination of toxic metal(oid)s and decabrominated diphenyl ether",
volume = "489",
doi = "10.1016/j.tox.2023.153496"
}

Radović, B., Stojilković, N., Ćurčić, M., Antonijević-Miljaković, E., Buha-Đorđević, A., Vukelić-Javorac, D., Baralić, K., Đukić-Ćosić, D., Bulat, Z.,& Antonijević, B.. (2023). In silico assessment of mixture toxicity mechanisms involved in the pathogenesis of thyroid diseases: The combination of toxic metal(oid)s and decabrominated diphenyl ether. in Toxicology
Elsevier Ireland Ltd., 489.
https://doi.org/10.1016/j.tox.2023.153496

Radović B, Stojilković N, Ćurčić M, Antonijević-Miljaković E, Buha-Đorđević A, Vukelić-Javorac D, Baralić K, Đukić-Ćosić D, Bulat Z, Antonijević B. In silico assessment of mixture toxicity mechanisms involved in the pathogenesis of thyroid diseases: The combination of toxic metal(oid)s and decabrominated diphenyl ether. in Toxicology. 2023;489.
doi:10.1016/j.tox.2023.153496 .

Radović, Biljana, Stojilković, Nikola, Ćurčić, Marijana, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Vukelić-Javorac, Dragana, Baralić, Katarina, Đukić-Ćosić, Danijela, Bulat, Zorica, Antonijević, Biljana, "In silico assessment of mixture toxicity mechanisms involved in the pathogenesis of thyroid diseases: The combination of toxic metal(oid)s and decabrominated diphenyl ether" in Toxicology, 489 (2023),
https://doi.org/10.1016/j.tox.2023.153496 . .

TY  - JOUR
AU  - Đukić-Ćosić, Danijela
AU  - Baralić, Katarina
AU  - Jorgovanović, Dragica
AU  - Živančević, Katarina
AU  - Javorac, Dragana
AU  - Stojilković, Nikola
AU  - Radović, Biljana
AU  - Marić, Đurđica
AU  - Ćurčić, Marijana
AU  - Buha-Đorđević, Aleksandra
AU  - Bulat, Zorica
AU  - Antonijević-Miljaković, Evica
AU  - Antonijević, Biljana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3958
AB  - While experimental animal investigation has historically been the most conventional
approach conducted to assess drug safety and is currently considered the main method for
determining drug toxicity, these studies are constricted by cost, time, and ethical approvals. Over
the last 20 years, there have been significant advances in computational sciences and computer
data processing, while knowledge of alternative techniques and their application has developed
into a valuable skill in toxicology. Thus, the application of in silico methods in drug safety
assessment is constantly increasing. They are very complex and are grounded on accumulated
knowledge from toxicology, bioinformatics, biochemistry, statistics, mathematics, as well as
molecular biology. This review will summarize current state-of-the-art scientific data on the use
of in silico methods in toxicity testing, taking into account their shortcomings, and highlighting
the strategies that should deliver consistent results, while covering the applications of in silico
methods in preclinical trials and drug impurities toxicity testing.
AB  - Ispitivanja na eksperimentalnim životinjama ne samo da su u prošlosti bila smatrana najkonvencionalnijim pristupom za procenu bezbednosti lekova, već su i trenutno osnovna metoda za utvrđivanje njihove toksičnosti. Međutim, ova ispitivanja su skupa, vremenski zahtevna i za njihovo sprovođenje neophodne su etičke dozvole. Tokom poslednjih 20 godina došlo je do napretka u računarskoj nauci i kompjuterskoj obradi podataka, dok se znanje o alternativnim tehnikama i njihovoj primeni razvilo u dragocenu veštinu u toksikologiji. Stoga, primena in silico metoda u proceni bezbednosti lekova neprestano raste. Ove metode su veoma složene i zasnivaju se na saznanjima iz toksikologije, bioinformatike, biohemije, statistike, matematike i molekularne biologije. Ovaj pregledni rad će rezimirati trenutna naučna saznanja koja se tiču upotrebe in silico metoda u ispitivanju toksičnosti lekova, uzimajući u obzir njihova ograničenja i ističući strategije pomoću kojih se mogu dobiti konzistentni rezultati, sa posebnim osvrtom na primenu in silico metoda u pretkliničkim ispitivanjima i ispitivanjima toksičnosti nečistoća u lekovima.
PB  - Beograd : Savez farmaceutskih udruženja Srbije
T2  - Arhiv za farmaciju
T1  - In silico toxicology methods in drug safety assessment
T1  - "In silico" metode u toksikologiji za procenu bezbednosti lekova
VL  - 71
IS  - 4
SP  - 257
EP  - 278
DO  - 10.5937/arhfarm71-32966
ER  - 

@article{
author = "Đukić-Ćosić, Danijela and Baralić, Katarina and Jorgovanović, Dragica and Živančević, Katarina and Javorac, Dragana and Stojilković, Nikola and Radović, Biljana and Marić, Đurđica and Ćurčić, Marijana and Buha-Đorđević, Aleksandra and Bulat, Zorica and Antonijević-Miljaković, Evica and Antonijević, Biljana",
year = "2021",
abstract = "While experimental animal investigation has historically been the most conventional
approach conducted to assess drug safety and is currently considered the main method for
determining drug toxicity, these studies are constricted by cost, time, and ethical approvals. Over
the last 20 years, there have been significant advances in computational sciences and computer
data processing, while knowledge of alternative techniques and their application has developed
into a valuable skill in toxicology. Thus, the application of in silico methods in drug safety
assessment is constantly increasing. They are very complex and are grounded on accumulated
knowledge from toxicology, bioinformatics, biochemistry, statistics, mathematics, as well as
molecular biology. This review will summarize current state-of-the-art scientific data on the use
of in silico methods in toxicity testing, taking into account their shortcomings, and highlighting
the strategies that should deliver consistent results, while covering the applications of in silico
methods in preclinical trials and drug impurities toxicity testing., Ispitivanja na eksperimentalnim životinjama ne samo da su u prošlosti bila smatrana najkonvencionalnijim pristupom za procenu bezbednosti lekova, već su i trenutno osnovna metoda za utvrđivanje njihove toksičnosti. Međutim, ova ispitivanja su skupa, vremenski zahtevna i za njihovo sprovođenje neophodne su etičke dozvole. Tokom poslednjih 20 godina došlo je do napretka u računarskoj nauci i kompjuterskoj obradi podataka, dok se znanje o alternativnim tehnikama i njihovoj primeni razvilo u dragocenu veštinu u toksikologiji. Stoga, primena in silico metoda u proceni bezbednosti lekova neprestano raste. Ove metode su veoma složene i zasnivaju se na saznanjima iz toksikologije, bioinformatike, biohemije, statistike, matematike i molekularne biologije. Ovaj pregledni rad će rezimirati trenutna naučna saznanja koja se tiču upotrebe in silico metoda u ispitivanju toksičnosti lekova, uzimajući u obzir njihova ograničenja i ističući strategije pomoću kojih se mogu dobiti konzistentni rezultati, sa posebnim osvrtom na primenu in silico metoda u pretkliničkim ispitivanjima i ispitivanjima toksičnosti nečistoća u lekovima.",
publisher = "Beograd : Savez farmaceutskih udruženja Srbije",
journal = "Arhiv za farmaciju",
title = "In silico toxicology methods in drug safety assessment, "In silico" metode u toksikologiji za procenu bezbednosti lekova",
volume = "71",
number = "4",
pages = "257-278",
doi = "10.5937/arhfarm71-32966"
}

Đukić-Ćosić, D., Baralić, K., Jorgovanović, D., Živančević, K., Javorac, D., Stojilković, N., Radović, B., Marić, Đ., Ćurčić, M., Buha-Đorđević, A., Bulat, Z., Antonijević-Miljaković, E.,& Antonijević, B.. (2021). In silico toxicology methods in drug safety assessment. in Arhiv za farmaciju
Beograd : Savez farmaceutskih udruženja Srbije., 71(4), 257-278.
https://doi.org/10.5937/arhfarm71-32966

Đukić-Ćosić D, Baralić K, Jorgovanović D, Živančević K, Javorac D, Stojilković N, Radović B, Marić Đ, Ćurčić M, Buha-Đorđević A, Bulat Z, Antonijević-Miljaković E, Antonijević B. In silico toxicology methods in drug safety assessment. in Arhiv za farmaciju. 2021;71(4):257-278.
doi:10.5937/arhfarm71-32966 .

Đukić-Ćosić, Danijela, Baralić, Katarina, Jorgovanović, Dragica, Živančević, Katarina, Javorac, Dragana, Stojilković, Nikola, Radović, Biljana, Marić, Đurđica, Ćurčić, Marijana, Buha-Đorđević, Aleksandra, Bulat, Zorica, Antonijević-Miljaković, Evica, Antonijević, Biljana, "In silico toxicology methods in drug safety assessment" in Arhiv za farmaciju, 71, no. 4 (2021):257-278,
https://doi.org/10.5937/arhfarm71-32966 . .