@article{
author = "Miseljić, Branislava and Popović, Gordana and Agbaba, Danica and Marković, Slavko and Simonovska, Breda and Vovk, Irena",
year = "2008",
abstract = "A gradient reversed-phase column high-performance liquid chromatographic method was developed for the detection and quantification of norfloxacin and its major impurities in norfloxacin-containing pharmaceuticals. Chromatographic separations were performed under the following experimental conditions: column, Zorbax SB RP-18 (5 mu m, 250 x 4.6 mm); injection volume, 20 mu L; mobile phase, 0.05 M NaH2PO4 (pH 2.5)-acetonitrile (87 + 13) for 16 min and (58 + 42) for 9 min (stepwise gradient); and flow rate, 1.3 mL/min. All analyses were performed at 25 degrees C, and the eluate was monitored at 275 nm using a diode array detector. Linearity (correlation coefficient = 0.999), recovery (99.3-101.8%), relative standard deviation (0.2-0.7%), and quantitation limit (0.12-0.47 mu g/mL) were evaluated and found to be satisfactory. The method is simple, rapid, and convenient for purity control of norfloxacin in both raw materials and dosage forms.",
publisher = "AOAC Int, Gaithersburg",
journal = "Journal of AOAC International",
title = "Column high-performance liquid chromatographic determination of norfloxacin and its main impurities in pharmaceuticals",
volume = "91",
number = "2",
pages = "332-338",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1077"
}