TY  - JOUR
AU  - Stefanović, Tatjana
AU  - Bosić, Srećko
AU  - Vekić, Jelena
AU  - Spasojević-Kalimanovska, Vesna
AU  - Todorović, Vesna
AU  - Pijanović, Marina
AU  - Zeljković, Aleksandra
AU  - Stefanović, Aleksandra
AU  - Jelić-Ivanović, Zorana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3667
AB  - Glikohemoglobin  (HbA1c)  je  važan  parametar  za  praćenje  dugoročne  metaboličke kontrole  i  procenu  rizika  za  razvoj  hroničnih  komplikacija  kod pacijenata  sa  dijabetes melitusom (DM). U ovoj studiji ispitivali smo povezanost postignute metaboličke kontrole u tipu  2  DM  sa  rizikom  za  razvoj  i  primenjenim  modalitetom  lečenja  sindroma  dijabetesnog stopala (SDS). U istraživanju je učestvovalo 111 pacijenata (80 muškaraca i 31 žena) sa tipom 2 DM, starosti 65,5 ± 9,8 godina. Kod 41 pacijenta SDS je lečen neoperativnim (konzervativnim) pristupom, hirurška intervencija manjeg obima (amputacija u predelu stopala) je izvršena kod 41,  a  opsežna  hirurška  intervencija  (amputacija  u  predelu  potkolenice)  kod  29  pacijenata. Koncentracije  biohemijskih  parametara  i  HbA1c  su  određene  standardnim  laboratorijskim metodama. U ispitivanoj grupi najučestaliji faktor rizika za nastanak SDS (82% pacijenata) bila je  neadekvatna  glikoregulacija  (HbA1c>8%).  Utvrdili  smo  da  su  pacijenti  koji  su  lečeni hirurškim putem imali statistički značajno više vrednosti HbA1c u poređenju sa pacijentima koji su  lečeni  neoperativnim  pristupom  (P<0,05).  Ukupan  broj  pacijenata  koji  je  podvrgnut hirurškom  lečenju  bio  je  značajno  veći  u  grupi  sa  neadekvatnom glikoregulacijom  (68,2% pacijenata  sa  neadekvatnom vs.  40%  pacijenata  sa  adekvatnom  glikoregulacijom; P<0,05). Utvrdili smo da je verovatnoća za amputaciju 3,2 puta veća ukoliko pacijent ima neadekvatnu glikoregulaciju  (OR=3,21;  95%CI:1,18-8,69; P<0,05).  Naši  rezultati  su  pokazali  da  se održavanjem dobre metaboličke kontrole u tipu 2 DM smanjuje rizik za razvoj SDS, ali i stepen invazivnosti postupka lečenja SDS.
AB  - Glycosylated hemoglobin (HbA1c) is important parameter for the assessment of long term metabolic control and risk for development of chronic complications in patients with diabetes mellitus (DM). This study investigates associations between achieved metabolic control in type 2 DM with the risk for the development of diabetic foot (DF) and its treatment modalities. The study included 111 patients (80 men and 31 women) with type 2 DM, aged 65.5±9.8 years. In 41 patients DF was treated by a conservative approach, 41 patients underwent a minor limb amputation (foot amputation) and 29 patients underwent a major limb amputation (below-knee amputation).  The  levels  of  HbA1c  and  serum  biochemical  parameters  were  measured  by standard laboratory methods. The most frequent risk factor for DF in the examined group (82% patients) was poor glycaemic control (HbA1c>8%). The level of HbA1c was significantly higher in surgically treated patients than in the conservatively treated group (P<0.05). The number of patients  who  underwent  surgical  treatment  were  significantly  higher  in  the  group  with  poor glycaemic control (62.8% patients with poor glycaemic control vs. 40% patients with optimal glycaemic control; P<0.05). We found that the patients with poor glycaemic control had 3.2 times  higher  risk  for  amputation  (OR=3.21;  95%CI:1.18-8.69; P<0.05).  In  conclusion,  our results  demonstrated  that  optimal  metabolic  control  in  type  2  DM  reduces  the  risk  for  DF development, as well as the invasiveness of DF treatment.
PB  - Pharmaceutical Association of Serbia
T2  - Arhiv za farmaciju
T1  - Significance of glycosylated haemoglobin determination for the assessment of lowerextremity amputation risk in patients with diabetic foot
T1  - Značaj određivanja glikohemoglobina u proceni rizika od amputacija kod pacijenata sa sindromom dijabetesnog stopala
VL  - 69
IS  - 2
SP  - 51
EP  - 66
DO  - 10.5937/arhfarm1902051X
ER  - 

@article{
author = "Stefanović, Tatjana and Bosić, Srećko and Vekić, Jelena and Spasojević-Kalimanovska, Vesna and Todorović, Vesna and Pijanović, Marina and Zeljković, Aleksandra and Stefanović, Aleksandra and Jelić-Ivanović, Zorana",
year = "2019",
abstract = "Glikohemoglobin  (HbA1c)  je  važan  parametar  za  praćenje  dugoročne  metaboličke kontrole  i  procenu  rizika  za  razvoj  hroničnih  komplikacija  kod pacijenata  sa  dijabetes melitusom (DM). U ovoj studiji ispitivali smo povezanost postignute metaboličke kontrole u tipu  2  DM  sa  rizikom  za  razvoj  i  primenjenim  modalitetom  lečenja  sindroma  dijabetesnog stopala (SDS). U istraživanju je učestvovalo 111 pacijenata (80 muškaraca i 31 žena) sa tipom 2 DM, starosti 65,5 ± 9,8 godina. Kod 41 pacijenta SDS je lečen neoperativnim (konzervativnim) pristupom, hirurška intervencija manjeg obima (amputacija u predelu stopala) je izvršena kod 41,  a  opsežna  hirurška  intervencija  (amputacija  u  predelu  potkolenice)  kod  29  pacijenata. Koncentracije  biohemijskih  parametara  i  HbA1c  su  određene  standardnim  laboratorijskim metodama. U ispitivanoj grupi najučestaliji faktor rizika za nastanak SDS (82% pacijenata) bila je  neadekvatna  glikoregulacija  (HbA1c>8%).  Utvrdili  smo  da  su  pacijenti  koji  su  lečeni hirurškim putem imali statistički značajno više vrednosti HbA1c u poređenju sa pacijentima koji su  lečeni  neoperativnim  pristupom  (P<0,05).  Ukupan  broj  pacijenata  koji  je  podvrgnut hirurškom  lečenju  bio  je  značajno  veći  u  grupi  sa  neadekvatnom glikoregulacijom  (68,2% pacijenata  sa  neadekvatnom vs.  40%  pacijenata  sa  adekvatnom  glikoregulacijom; P<0,05). Utvrdili smo da je verovatnoća za amputaciju 3,2 puta veća ukoliko pacijent ima neadekvatnu glikoregulaciju  (OR=3,21;  95%CI:1,18-8,69; P<0,05).  Naši  rezultati  su  pokazali  da  se održavanjem dobre metaboličke kontrole u tipu 2 DM smanjuje rizik za razvoj SDS, ali i stepen invazivnosti postupka lečenja SDS., Glycosylated hemoglobin (HbA1c) is important parameter for the assessment of long term metabolic control and risk for development of chronic complications in patients with diabetes mellitus (DM). This study investigates associations between achieved metabolic control in type 2 DM with the risk for the development of diabetic foot (DF) and its treatment modalities. The study included 111 patients (80 men and 31 women) with type 2 DM, aged 65.5±9.8 years. In 41 patients DF was treated by a conservative approach, 41 patients underwent a minor limb amputation (foot amputation) and 29 patients underwent a major limb amputation (below-knee amputation).  The  levels  of  HbA1c  and  serum  biochemical  parameters  were  measured  by standard laboratory methods. The most frequent risk factor for DF in the examined group (82% patients) was poor glycaemic control (HbA1c>8%). The level of HbA1c was significantly higher in surgically treated patients than in the conservatively treated group (P<0.05). The number of patients  who  underwent  surgical  treatment  were  significantly  higher  in  the  group  with  poor glycaemic control (62.8% patients with poor glycaemic control vs. 40% patients with optimal glycaemic control; P<0.05). We found that the patients with poor glycaemic control had 3.2 times  higher  risk  for  amputation  (OR=3.21;  95%CI:1.18-8.69; P<0.05).  In  conclusion,  our results  demonstrated  that  optimal  metabolic  control  in  type  2  DM  reduces  the  risk  for  DF development, as well as the invasiveness of DF treatment.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za farmaciju",
title = "Significance of glycosylated haemoglobin determination for the assessment of lowerextremity amputation risk in patients with diabetic foot, Značaj određivanja glikohemoglobina u proceni rizika od amputacija kod pacijenata sa sindromom dijabetesnog stopala",
volume = "69",
number = "2",
pages = "51-66",
doi = "10.5937/arhfarm1902051X"
}

Stefanović, T., Bosić, S., Vekić, J., Spasojević-Kalimanovska, V., Todorović, V., Pijanović, M., Zeljković, A., Stefanović, A.,& Jelić-Ivanović, Z.. (2019). Significance of glycosylated haemoglobin determination for the assessment of lowerextremity amputation risk in patients with diabetic foot. in Arhiv za farmaciju
Pharmaceutical Association of Serbia., 69(2), 51-66.
https://doi.org/10.5937/arhfarm1902051X

Stefanović T, Bosić S, Vekić J, Spasojević-Kalimanovska V, Todorović V, Pijanović M, Zeljković A, Stefanović A, Jelić-Ivanović Z. Significance of glycosylated haemoglobin determination for the assessment of lowerextremity amputation risk in patients with diabetic foot. in Arhiv za farmaciju. 2019;69(2):51-66.
doi:10.5937/arhfarm1902051X .

Stefanović, Tatjana, Bosić, Srećko, Vekić, Jelena, Spasojević-Kalimanovska, Vesna, Todorović, Vesna, Pijanović, Marina, Zeljković, Aleksandra, Stefanović, Aleksandra, Jelić-Ivanović, Zorana, "Significance of glycosylated haemoglobin determination for the assessment of lowerextremity amputation risk in patients with diabetic foot" in Arhiv za farmaciju, 69, no. 2 (2019):51-66,
https://doi.org/10.5937/arhfarm1902051X . .

TY  - JOUR
AU  - Pijanović, Marina
AU  - Stefanović, Aleksandra
AU  - Miljković, Milica
AU  - Marić-Krejović, Snežana
AU  - Spasić, Slavica
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2986
AB  - Background: Leptin and adiponectin play an important role during normal gestation; they are implicated in energy metabolism, glucose utilization and inflammation. Osteocalcin is released into circulation during bone formation; it also affects glucose metabolism by regulating insulin secretion and sensitivity, possibly mediated by adiponectin. The aim of this study was to explore the longitudinal changes of leptin and adiponectin in pregnancy, and their associations with lipid profile, insulin and bone formation parameters in late pregnancy. Methods: Leptin, adiponectin, lipid status parameters, C-reactive protein (CRP), insulin, 25-hydroxyvitamin D, osteocalcin and procollagen type 1 aminoterminal propeptide (P1NP) were measured in the sera of 38 healthy pregnant women. The samples were obtained in the 1st , 2nd, early and late 3rd, trimester, and post-partum. Results: Leptin was significantly increased in the 3rd trimester. The decrease of adiponectin was significant only in postpartum. Osteocalcin and P1NP increased in the late 3rd trimester and postpartum. Leptin was significantly positively correlated with body mass index (BMI), uric acid, insulin, osteocalcin, P1NP and CRP in the 3rd trimester; adiponectin was positively correlated with highdensity lipoprotein (HDL) cholesterol, and negatively with BMI, glucose, osteocalcin, triglycerides and insulin. Multiple regression analysis showed that only HDL is independently associated with adiponectin. Conclusions: The results of our study suggest complex interactions of leptin and adiponectin with glucose, lipid and bone metabolism during pregnancy. Adiponectin might be part of the protective systems that counterbalance a transient proatherogenic state observed in pregnancy mainly by improving the HDL levels. The exact mechanisms and potential implications in pathological states of pregnancy remain unexplained and require further investigation.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Laboratoriumsmedizin-Journal of Laboratory Medicine
T1  - Longitudinal changes in leptin and adiponectin concentrations through uncomplicated pregnancy
VL  - 41
IS  - 3
SP  - 129
EP  - 136
DO  - 10.1515/labmed-2017-0052
ER  - 

@article{
author = "Pijanović, Marina and Stefanović, Aleksandra and Miljković, Milica and Marić-Krejović, Snežana and Spasić, Slavica",
year = "2017",
abstract = "Background: Leptin and adiponectin play an important role during normal gestation; they are implicated in energy metabolism, glucose utilization and inflammation. Osteocalcin is released into circulation during bone formation; it also affects glucose metabolism by regulating insulin secretion and sensitivity, possibly mediated by adiponectin. The aim of this study was to explore the longitudinal changes of leptin and adiponectin in pregnancy, and their associations with lipid profile, insulin and bone formation parameters in late pregnancy. Methods: Leptin, adiponectin, lipid status parameters, C-reactive protein (CRP), insulin, 25-hydroxyvitamin D, osteocalcin and procollagen type 1 aminoterminal propeptide (P1NP) were measured in the sera of 38 healthy pregnant women. The samples were obtained in the 1st , 2nd, early and late 3rd, trimester, and post-partum. Results: Leptin was significantly increased in the 3rd trimester. The decrease of adiponectin was significant only in postpartum. Osteocalcin and P1NP increased in the late 3rd trimester and postpartum. Leptin was significantly positively correlated with body mass index (BMI), uric acid, insulin, osteocalcin, P1NP and CRP in the 3rd trimester; adiponectin was positively correlated with highdensity lipoprotein (HDL) cholesterol, and negatively with BMI, glucose, osteocalcin, triglycerides and insulin. Multiple regression analysis showed that only HDL is independently associated with adiponectin. Conclusions: The results of our study suggest complex interactions of leptin and adiponectin with glucose, lipid and bone metabolism during pregnancy. Adiponectin might be part of the protective systems that counterbalance a transient proatherogenic state observed in pregnancy mainly by improving the HDL levels. The exact mechanisms and potential implications in pathological states of pregnancy remain unexplained and require further investigation.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Laboratoriumsmedizin-Journal of Laboratory Medicine",
title = "Longitudinal changes in leptin and adiponectin concentrations through uncomplicated pregnancy",
volume = "41",
number = "3",
pages = "129-136",
doi = "10.1515/labmed-2017-0052"
}

Pijanović, M., Stefanović, A., Miljković, M., Marić-Krejović, S.,& Spasić, S.. (2017). Longitudinal changes in leptin and adiponectin concentrations through uncomplicated pregnancy. in Laboratoriumsmedizin-Journal of Laboratory Medicine
Walter de Gruyter Gmbh, Berlin., 41(3), 129-136.
https://doi.org/10.1515/labmed-2017-0052

Pijanović M, Stefanović A, Miljković M, Marić-Krejović S, Spasić S. Longitudinal changes in leptin and adiponectin concentrations through uncomplicated pregnancy. in Laboratoriumsmedizin-Journal of Laboratory Medicine. 2017;41(3):129-136.
doi:10.1515/labmed-2017-0052 .

Pijanović, Marina, Stefanović, Aleksandra, Miljković, Milica, Marić-Krejović, Snežana, Spasić, Slavica, "Longitudinal changes in leptin and adiponectin concentrations through uncomplicated pregnancy" in Laboratoriumsmedizin-Journal of Laboratory Medicine, 41, no. 3 (2017):129-136,
https://doi.org/10.1515/labmed-2017-0052 . .

TY  - JOUR
AU  - Pijanović, Marina
AU  - Stefanović, Aleksandra
AU  - Miljković, Milica
AU  - Marić-Krejović, Snežana
AU  - Spasić, Slavica
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2717
AB  - Background: The aim of this study was to explore longitudinal changes of serum osteocalcin during normal, uncomplicated pregnancy and after delivery, and its correlations with parameters of glucose homeostasis, lipid status, and oxidative status in late pregnancy. Methods: Osteocalcin, glucose, insulin, lipid status parameters, total oxidative status (TOS), and total antioxidant capacity (TAC) were measured in sera of 38 healthy pregnant women. The sera were collected at the midpoint of the 1st, in the 2nd and 3rd trimester, and after delivery. Homeostatic model assessment (HOMA) indices were calculated and used as surrogate markers of insulin resistance. Results: Repeated measures analysis of variance showed a progressive increase in total cholesterol, triglycerides, and low density lipoprotein (LDL)-cholesterol, with a postpartum decrease. High density lipoprotein (HDL)-cholesterol increased in the 2nd trimester and decreased after delivery. Total oxidative status (TOS) increased significantly in the 3rd trimester (p  lt  0.001). TAC showed a significant increase after delivery (p  lt  0.05). Insulin showed a significant increase in the 3rd trimester (p  lt  0.05). Homeostatic model assessment (HOMA)-%B increased significantly in the 3rd trimester (p  lt  0.001). Osteocalcin showed a decrease in the 2nd trimester, and a marked increase in the 3rd - trimester and postpartum (p  lt  0.001). Osteocalcin was significantly positively correlated with BMI, insulin, HOMA of insulin resistance (HOMA-IR), HOMA-%B, TAC (p  lt  0.05), triglycerides and uric acid (p  lt  0.001). Multiple regression analysis showed that TAC is independently associated with osteocalcin level during 3rd trimester (p  lt  0.05). Conclusions: We observed the changes in pregnancy that may lead towards atherogenic, prooxidant and insulin resistant state, which are possibly counterbalanced by various protective systems, one of which might be osteocalcin.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Laboratoriumsmedizin-Journal of Laboratory Medicine
T1  - Association of osteocalcin, insulin resistance and oxidative stress during noncomplicated pregnancy
VL  - 40
IS  - 4
SP  - 247
EP  - 253
DO  - 10.1515/labmed-2016-0024
ER  - 

@article{
author = "Pijanović, Marina and Stefanović, Aleksandra and Miljković, Milica and Marić-Krejović, Snežana and Spasić, Slavica",
year = "2016",
abstract = "Background: The aim of this study was to explore longitudinal changes of serum osteocalcin during normal, uncomplicated pregnancy and after delivery, and its correlations with parameters of glucose homeostasis, lipid status, and oxidative status in late pregnancy. Methods: Osteocalcin, glucose, insulin, lipid status parameters, total oxidative status (TOS), and total antioxidant capacity (TAC) were measured in sera of 38 healthy pregnant women. The sera were collected at the midpoint of the 1st, in the 2nd and 3rd trimester, and after delivery. Homeostatic model assessment (HOMA) indices were calculated and used as surrogate markers of insulin resistance. Results: Repeated measures analysis of variance showed a progressive increase in total cholesterol, triglycerides, and low density lipoprotein (LDL)-cholesterol, with a postpartum decrease. High density lipoprotein (HDL)-cholesterol increased in the 2nd trimester and decreased after delivery. Total oxidative status (TOS) increased significantly in the 3rd trimester (p  lt  0.001). TAC showed a significant increase after delivery (p  lt  0.05). Insulin showed a significant increase in the 3rd trimester (p  lt  0.05). Homeostatic model assessment (HOMA)-%B increased significantly in the 3rd trimester (p  lt  0.001). Osteocalcin showed a decrease in the 2nd trimester, and a marked increase in the 3rd - trimester and postpartum (p  lt  0.001). Osteocalcin was significantly positively correlated with BMI, insulin, HOMA of insulin resistance (HOMA-IR), HOMA-%B, TAC (p  lt  0.05), triglycerides and uric acid (p  lt  0.001). Multiple regression analysis showed that TAC is independently associated with osteocalcin level during 3rd trimester (p  lt  0.05). Conclusions: We observed the changes in pregnancy that may lead towards atherogenic, prooxidant and insulin resistant state, which are possibly counterbalanced by various protective systems, one of which might be osteocalcin.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Laboratoriumsmedizin-Journal of Laboratory Medicine",
title = "Association of osteocalcin, insulin resistance and oxidative stress during noncomplicated pregnancy",
volume = "40",
number = "4",
pages = "247-253",
doi = "10.1515/labmed-2016-0024"
}

Pijanović, M., Stefanović, A., Miljković, M., Marić-Krejović, S.,& Spasić, S.. (2016). Association of osteocalcin, insulin resistance and oxidative stress during noncomplicated pregnancy. in Laboratoriumsmedizin-Journal of Laboratory Medicine
Walter de Gruyter Gmbh, Berlin., 40(4), 247-253.
https://doi.org/10.1515/labmed-2016-0024

Pijanović M, Stefanović A, Miljković M, Marić-Krejović S, Spasić S. Association of osteocalcin, insulin resistance and oxidative stress during noncomplicated pregnancy. in Laboratoriumsmedizin-Journal of Laboratory Medicine. 2016;40(4):247-253.
doi:10.1515/labmed-2016-0024 .

Pijanović, Marina, Stefanović, Aleksandra, Miljković, Milica, Marić-Krejović, Snežana, Spasić, Slavica, "Association of osteocalcin, insulin resistance and oxidative stress during noncomplicated pregnancy" in Laboratoriumsmedizin-Journal of Laboratory Medicine, 40, no. 4 (2016):247-253,
https://doi.org/10.1515/labmed-2016-0024 . .