TY  - CONF
AU  - Mirković, Kristina
AU  - Aranđelović, Jovana
AU  - Kojić, Jana
AU  - Stevanović, Vladimir
AU  - Batinić, Bojan
AU  - Todorović, Vanja
AU  - Đoković, Jelena
AU  - Santrač, Anja
AU  - Major, Tamara
AU  - Savić, Miroslav
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5521
AB  - Introduction: Ciprofloxacin is a fluoroquinolone antibiotic commonly used to treat various bacterial infections, with a potential to induce adverse mood effects in patients. Since the molecular mechanism of ciprofloxacin-induced neurotoxicity is poorly understood, we aimed to identify behavioral changes and corresponding neurotransmitter pattern after its prolonged administration in rats. We screened for untoward effects of ciprofloxacin on locomotor activity, despair, anhedonia, object recognition memory, and anxiety, as behavioral domains affected in various psychiatric diseases. Methodology: Three-month old male Sprague-Dawley rats were orally gavaged with ciprofloxacin (20 or 100 mg/kg) or solvent (0.5% methyl cellulose solution) each day for 4 weeks (n=80). One group of animals (n=40) passed the open field (OF), novel object recognition test (NORT), and forced swimming test (FST). Another group (n=40) underwent elevated plus maze (EPM) and sucrose preference test (SPT). After the completion of behavioral battery, the prefrontal cortex and cerebrospinal fluid (CSF) were collected. The neurotransmitters and metabolites of the kynurenine pathway were determined in the prefrontal cortex (PFC) through HPLC-MS/MS. Additionally, levels of interleukin-2 (IL-2) in CSF were quantified with Luminex. Behavioral and molecular parameters were analyzed by one-way ANOVA followed by Dunnett post hoc test in GraphPad Prism 9. Results: In FST, the treatment with high dose of ciprofloxacin decreased the swim time compared to control, which could be related to induction of despair-like behavior (p<0.05). The ciprofloxacin treatment did not affect object memory in NORT. In OF, the distance travelled and the number of rotations were not changed after treatment with ciprofloxacin compared to the control group. Further, animals treated with ciprofloxacin did not show changes in parameters in EPM and SPT. The acetylcholine levels in PFC were increased after ciprofloxacin treatment (p<0.05) in comparison with controls, which could be associated with depressed mood states. In line with that, high dose of ciprofloxacin treatment showed the tendency to decrease and increase levels of GABA and dopamine, respectively, but without reaching the statistical significance (p=0.07 and p=0.06). No changes in kynurenine pathway were observed after the treatment. The IL-2 concentration in CSF was increased after prolonged administration of low dose of ciprofloxacin treatment compared to the control levels (p<0.05), which could imply immunological stimulation of T lymphocytes and potential neuroinflammation. Conclusion: The despair behavior after treatment with high dose of ciprofloxacin was accompanied by increased levels of acetylcholine in PFC. Furthermore, the high dose of ciprofloxacin treatment showed tendency to decrease GABA levels, and increase dopamine levels in PFC, which could be connected to psychiatric adverse effects. Nonetheless, further studies are essential to confirm these neurotransmitter changes. On the other hand, the low dose of ciprofloxacin treatment elicited the increase of IL-2, which could be a marker of neuroinflammation-related neurotoxicity. In the future, efforts should be made to examine the role of IL-2 in the interaction of the immune system and the central nervous system, as its potential significance as a biomarker.
PB  - European College of Neuropsychopharmacology (ECNP)
C3  - 36th ECPN congress, 7th -10th October 2023, Barcelona, Spain
T1  - Deciphering ciprofloxacin-induced neurotoxicity: behavioral and molecular profiling of ciprofloxacin treatment in rats
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5521
ER  - 

@conference{
author = "Mirković, Kristina and Aranđelović, Jovana and Kojić, Jana and Stevanović, Vladimir and Batinić, Bojan and Todorović, Vanja and Đoković, Jelena and Santrač, Anja and Major, Tamara and Savić, Miroslav",
year = "2023",
abstract = "Introduction: Ciprofloxacin is a fluoroquinolone antibiotic commonly used to treat various bacterial infections, with a potential to induce adverse mood effects in patients. Since the molecular mechanism of ciprofloxacin-induced neurotoxicity is poorly understood, we aimed to identify behavioral changes and corresponding neurotransmitter pattern after its prolonged administration in rats. We screened for untoward effects of ciprofloxacin on locomotor activity, despair, anhedonia, object recognition memory, and anxiety, as behavioral domains affected in various psychiatric diseases. Methodology: Three-month old male Sprague-Dawley rats were orally gavaged with ciprofloxacin (20 or 100 mg/kg) or solvent (0.5% methyl cellulose solution) each day for 4 weeks (n=80). One group of animals (n=40) passed the open field (OF), novel object recognition test (NORT), and forced swimming test (FST). Another group (n=40) underwent elevated plus maze (EPM) and sucrose preference test (SPT). After the completion of behavioral battery, the prefrontal cortex and cerebrospinal fluid (CSF) were collected. The neurotransmitters and metabolites of the kynurenine pathway were determined in the prefrontal cortex (PFC) through HPLC-MS/MS. Additionally, levels of interleukin-2 (IL-2) in CSF were quantified with Luminex. Behavioral and molecular parameters were analyzed by one-way ANOVA followed by Dunnett post hoc test in GraphPad Prism 9. Results: In FST, the treatment with high dose of ciprofloxacin decreased the swim time compared to control, which could be related to induction of despair-like behavior (p<0.05). The ciprofloxacin treatment did not affect object memory in NORT. In OF, the distance travelled and the number of rotations were not changed after treatment with ciprofloxacin compared to the control group. Further, animals treated with ciprofloxacin did not show changes in parameters in EPM and SPT. The acetylcholine levels in PFC were increased after ciprofloxacin treatment (p<0.05) in comparison with controls, which could be associated with depressed mood states. In line with that, high dose of ciprofloxacin treatment showed the tendency to decrease and increase levels of GABA and dopamine, respectively, but without reaching the statistical significance (p=0.07 and p=0.06). No changes in kynurenine pathway were observed after the treatment. The IL-2 concentration in CSF was increased after prolonged administration of low dose of ciprofloxacin treatment compared to the control levels (p<0.05), which could imply immunological stimulation of T lymphocytes and potential neuroinflammation. Conclusion: The despair behavior after treatment with high dose of ciprofloxacin was accompanied by increased levels of acetylcholine in PFC. Furthermore, the high dose of ciprofloxacin treatment showed tendency to decrease GABA levels, and increase dopamine levels in PFC, which could be connected to psychiatric adverse effects. Nonetheless, further studies are essential to confirm these neurotransmitter changes. On the other hand, the low dose of ciprofloxacin treatment elicited the increase of IL-2, which could be a marker of neuroinflammation-related neurotoxicity. In the future, efforts should be made to examine the role of IL-2 in the interaction of the immune system and the central nervous system, as its potential significance as a biomarker.",
publisher = "European College of Neuropsychopharmacology (ECNP)",
journal = "36th ECPN congress, 7th -10th October 2023, Barcelona, Spain",
title = "Deciphering ciprofloxacin-induced neurotoxicity: behavioral and molecular profiling of ciprofloxacin treatment in rats",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5521"
}

Mirković, K., Aranđelović, J., Kojić, J., Stevanović, V., Batinić, B., Todorović, V., Đoković, J., Santrač, A., Major, T.,& Savić, M.. (2023). Deciphering ciprofloxacin-induced neurotoxicity: behavioral and molecular profiling of ciprofloxacin treatment in rats. in 36th ECPN congress, 7th -10th October 2023, Barcelona, Spain
European College of Neuropsychopharmacology (ECNP)..
https://hdl.handle.net/21.15107/rcub_farfar_5521

Mirković K, Aranđelović J, Kojić J, Stevanović V, Batinić B, Todorović V, Đoković J, Santrač A, Major T, Savić M. Deciphering ciprofloxacin-induced neurotoxicity: behavioral and molecular profiling of ciprofloxacin treatment in rats. in 36th ECPN congress, 7th -10th October 2023, Barcelona, Spain. 2023;.
https://hdl.handle.net/21.15107/rcub_farfar_5521 .

Mirković, Kristina, Aranđelović, Jovana, Kojić, Jana, Stevanović, Vladimir, Batinić, Bojan, Todorović, Vanja, Đoković, Jelena, Santrač, Anja, Major, Tamara, Savić, Miroslav, "Deciphering ciprofloxacin-induced neurotoxicity: behavioral and molecular profiling of ciprofloxacin treatment in rats" in 36th ECPN congress, 7th -10th October 2023, Barcelona, Spain (2023),
https://hdl.handle.net/21.15107/rcub_farfar_5521 .

TY  - JOUR
AU  - Divović-Matović, Branka
AU  - Knutson, Dan
AU  - Mitrović, Jelena
AU  - Stevanović, Vladimir
AU  - Stanojević, Boban
AU  - Savić, Snežana
AU  - Cook, James
AU  - Savić, Miroslav
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4289
AB  - Several pyrazoloquinolinone (PQ) ligands were recently discovered as func-tionally selective positive modulators at the PQ site ofα6-containing GABAAreceptors. PQs are also neutral modulators at the benzodiazepine site. Weassessed the influence of PQ compounds from three structural groups (PZ-II-029 and related deuterated analogues DK-I-56-1, RV-I-029, DK-I-60-3 and DK-I-86-1; LAU 463 and related analogues DK-I-58-1 and DK-II-58-1; and DK-I-87-1), alone and in combination with diazepam, on the behaviour of maleSprague–Dawley rats. An excellent behavioural safety profile of all tested PQswas demonstrated in the spontaneous locomotor activity, rotarod, loss of right-ing reflex and pentylenetetrazol tests. In interaction studies, only PZ-II-029and its analogues prevented the ataxic effects of the benzodiazepine, asassessed in the rotarod test and during monitoring of rat locomotor activityafter awakening from the loss of righting reflex. Published electrophysiologicalprofiles of PQ ligands imply that positive modulation elicited atα6-GABAAreceptors that contain theγ2 andδsubunit, rather than their neutral modula-tory action at the benzodiazepine site, may prevent the ataxic action of diaze-pam. Thus, PZ-II-029 and its deuterated analogues are not prone to untowardinteractions with benzodiazepines and may indeed completely abolish theirataxic action, seen at therapeutic, and especially toxic concentrations.
PB  - John Wiley and Sons Inc
T2  - Basic and Clinical Pharmacology and Toxicology
T1  - Behavioural interaction of pyrazoloquinolinone positive allosteric modulators at α6GABAA receptors and diazepam in rats: Anti-diazepam-induced ataxia action as a structure-dependent feature
VL  - 131
IS  - 6
SP  - 514
EP  - 524
DO  - 10.1111/bcpt.13801
ER  - 

@article{
author = "Divović-Matović, Branka and Knutson, Dan and Mitrović, Jelena and Stevanović, Vladimir and Stanojević, Boban and Savić, Snežana and Cook, James and Savić, Miroslav",
year = "2022",
abstract = "Several pyrazoloquinolinone (PQ) ligands were recently discovered as func-tionally selective positive modulators at the PQ site ofα6-containing GABAAreceptors. PQs are also neutral modulators at the benzodiazepine site. Weassessed the influence of PQ compounds from three structural groups (PZ-II-029 and related deuterated analogues DK-I-56-1, RV-I-029, DK-I-60-3 and DK-I-86-1; LAU 463 and related analogues DK-I-58-1 and DK-II-58-1; and DK-I-87-1), alone and in combination with diazepam, on the behaviour of maleSprague–Dawley rats. An excellent behavioural safety profile of all tested PQswas demonstrated in the spontaneous locomotor activity, rotarod, loss of right-ing reflex and pentylenetetrazol tests. In interaction studies, only PZ-II-029and its analogues prevented the ataxic effects of the benzodiazepine, asassessed in the rotarod test and during monitoring of rat locomotor activityafter awakening from the loss of righting reflex. Published electrophysiologicalprofiles of PQ ligands imply that positive modulation elicited atα6-GABAAreceptors that contain theγ2 andδsubunit, rather than their neutral modula-tory action at the benzodiazepine site, may prevent the ataxic action of diaze-pam. Thus, PZ-II-029 and its deuterated analogues are not prone to untowardinteractions with benzodiazepines and may indeed completely abolish theirataxic action, seen at therapeutic, and especially toxic concentrations.",
publisher = "John Wiley and Sons Inc",
journal = "Basic and Clinical Pharmacology and Toxicology",
title = "Behavioural interaction of pyrazoloquinolinone positive allosteric modulators at α6GABAA receptors and diazepam in rats: Anti-diazepam-induced ataxia action as a structure-dependent feature",
volume = "131",
number = "6",
pages = "514-524",
doi = "10.1111/bcpt.13801"
}

Divović-Matović, B., Knutson, D., Mitrović, J., Stevanović, V., Stanojević, B., Savić, S., Cook, J.,& Savić, M.. (2022). Behavioural interaction of pyrazoloquinolinone positive allosteric modulators at α6GABAA receptors and diazepam in rats: Anti-diazepam-induced ataxia action as a structure-dependent feature. in Basic and Clinical Pharmacology and Toxicology
John Wiley and Sons Inc., 131(6), 514-524.
https://doi.org/10.1111/bcpt.13801

Divović-Matović B, Knutson D, Mitrović J, Stevanović V, Stanojević B, Savić S, Cook J, Savić M. Behavioural interaction of pyrazoloquinolinone positive allosteric modulators at α6GABAA receptors and diazepam in rats: Anti-diazepam-induced ataxia action as a structure-dependent feature. in Basic and Clinical Pharmacology and Toxicology. 2022;131(6):514-524.
doi:10.1111/bcpt.13801 .

Divović-Matović, Branka, Knutson, Dan, Mitrović, Jelena, Stevanović, Vladimir, Stanojević, Boban, Savić, Snežana, Cook, James, Savić, Miroslav, "Behavioural interaction of pyrazoloquinolinone positive allosteric modulators at α6GABAA receptors and diazepam in rats: Anti-diazepam-induced ataxia action as a structure-dependent feature" in Basic and Clinical Pharmacology and Toxicology, 131, no. 6 (2022):514-524,
https://doi.org/10.1111/bcpt.13801 . .

TY  - JOUR
AU  - Niketić, Marjan
AU  - Tomović, Gordana
AU  - Anačkov, Goran
AU  - Đorđević, Vladan
AU  - Đurović, Sanja
AU  - Duraki, Šemija
AU  - Kabaš, Eva
AU  - Lakušić, Dmitar
AU  - Petkovski, Gorana
AU  - Petrović, Silvana
AU  - Ranimirović, Milana
AU  - Slavkovska, Violeta
AU  - Ušjak, Ljuboš
AU  - Zbiljić, Miloš
AU  - Zlatković, Bojan
AU  - Stevanović, Vladimir
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4958
AB  - This paper represents the fourth part of the inventory of the flora of Serbia (Niketić et al. 2018, 2020, 2021), which contains nomenclatural, taxonomic and floristic notes related to taxa from the Magnoliopsida group. At the same time, this contribution is the basis for publication of subsequent volumes of the An annotated checklist of vascular flora of Serbia in order to supplement the data on vascular plants in our country.
AB  - У раду је предложено пет нових номенклатурних комбинација, а поред тога, представљено је 17 таксона (врста, подврста и хибрида) васкуларне флоре који су нови за флору Србије (од тога 12 аутохтоних и пет алохтоних биљак). Потврђени су стари или непоуздани наводи за две аутохтоне биљке у флори Србије. У последњем делу рада дате су информације о деветнаест таксона чије је присуство у Србији или у њеним територијалним јединицама оповргнуто.
PB  - Natural History Museum, Belgrade, Serbia
T2  - Bulletin of the Natural History Museum
T1  - Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV
T1  - Mатеријал за Kритичку листу врста васкуларне флоре Србије. Номенклатурни, таксономски и флористички прилози IV
VL  - 15
SP  - 27
EP  - 96
DO  - 10.5937/bnhmb2215027N
ER  - 

@article{
author = "Niketić, Marjan and Tomović, Gordana and Anačkov, Goran and Đorđević, Vladan and Đurović, Sanja and Duraki, Šemija and Kabaš, Eva and Lakušić, Dmitar and Petkovski, Gorana and Petrović, Silvana and Ranimirović, Milana and Slavkovska, Violeta and Ušjak, Ljuboš and Zbiljić, Miloš and Zlatković, Bojan and Stevanović, Vladimir",
year = "2022",
abstract = "This paper represents the fourth part of the inventory of the flora of Serbia (Niketić et al. 2018, 2020, 2021), which contains nomenclatural, taxonomic and floristic notes related to taxa from the Magnoliopsida group. At the same time, this contribution is the basis for publication of subsequent volumes of the An annotated checklist of vascular flora of Serbia in order to supplement the data on vascular plants in our country., У раду је предложено пет нових номенклатурних комбинација, а поред тога, представљено је 17 таксона (врста, подврста и хибрида) васкуларне флоре који су нови за флору Србије (од тога 12 аутохтоних и пет алохтоних биљак). Потврђени су стари или непоуздани наводи за две аутохтоне биљке у флори Србије. У последњем делу рада дате су информације о деветнаест таксона чије је присуство у Србији или у њеним територијалним јединицама оповргнуто.",
publisher = "Natural History Museum, Belgrade, Serbia",
journal = "Bulletin of the Natural History Museum",
title = "Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV, Mатеријал за Kритичку листу врста васкуларне флоре Србије. Номенклатурни, таксономски и флористички прилози IV",
volume = "15",
pages = "27-96",
doi = "10.5937/bnhmb2215027N"
}

Niketić, M., Tomović, G., Anačkov, G., Đorđević, V., Đurović, S., Duraki, Š., Kabaš, E., Lakušić, D., Petkovski, G., Petrović, S., Ranimirović, M., Slavkovska, V., Ušjak, L., Zbiljić, M., Zlatković, B.,& Stevanović, V.. (2022). Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV. in Bulletin of the Natural History Museum
Natural History Museum, Belgrade, Serbia., 15, 27-96.
https://doi.org/10.5937/bnhmb2215027N

Niketić M, Tomović G, Anačkov G, Đorđević V, Đurović S, Duraki Š, Kabaš E, Lakušić D, Petkovski G, Petrović S, Ranimirović M, Slavkovska V, Ušjak L, Zbiljić M, Zlatković B, Stevanović V. Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV. in Bulletin of the Natural History Museum. 2022;15:27-96.
doi:10.5937/bnhmb2215027N .

Niketić, Marjan, Tomović, Gordana, Anačkov, Goran, Đorđević, Vladan, Đurović, Sanja, Duraki, Šemija, Kabaš, Eva, Lakušić, Dmitar, Petkovski, Gorana, Petrović, Silvana, Ranimirović, Milana, Slavkovska, Violeta, Ušjak, Ljuboš, Zbiljić, Miloš, Zlatković, Bojan, Stevanović, Vladimir, "Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV" in Bulletin of the Natural History Museum, 15 (2022):27-96,
https://doi.org/10.5937/bnhmb2215027N . .

TY  - JOUR
AU  - Niketić, Marjan
AU  - Tomović, Gordana
AU  - Bokić, Bojana
AU  - Buzurović, Uroš
AU  - Duraki, Šemija
AU  - Đorđević, Vladan
AU  - Đurović, Sanja
AU  - Krivošej, Zoran
AU  - Lazarević, Predrag
AU  - Perić, Ranko
AU  - Prodanović, Danijela
AU  - Radak, Boris
AU  - Rat, Milica
AU  - Ranimirović, Milana
AU  - Stevanović, Vladimir
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5445
AB  - The paper proposes eight new nomenclatural combinations and indicates two 
new synonyms. There are 11 taxa (species and subspecies) of the vascular flora 
that are new for the flora of Serbia (of which nine are autochthonous and two 
allochthonous plants). Two autochthonous species were confirmed for the flora of 
Serbia. Six plant taxa are a novelty for proper Serbia, Vojvodina, or Kosovo and 
Metohija. There are eight taxa whose presence in Serbia or in its territorial units has
been refuted.
AB  - У раду је предложено осам нових номенклатурних комбинација и 
назначена су два нова синонима. Поред тога, представљено је 11
таксона (врста и подврста) васкуларне флоре који су нови за флору 
Србије (од тога девет аутохтоних и две алохтоне биљке). Потврђено је 
и присуство две васкуларне биљке за флору Србије. Шест биљних 
таксона представљају новину за ужу Србију, Војводину или Косово и 
Метохију. Такође је присуство неких таксона у Србији или у њеним 
територијалним јединицама оповргнуто.
PB  - Natural History Museum, Belgrade, Serbia
T2  - Bulletin of the Natural History Museum
T1  - Material on the Annotated Checklist of Vascular Flora of Serbia. Nomenclatural, taxonomic and floristic notes III
T1  - Материјал за kритичку листу врста васкуларне флоре Србије. номенклатурни, таксономски и флористички прилози III
VL  - 14
SP  - 77
EP  - 132
DO  - 10.5937/bnhmb2114077N
ER  - 

@article{
author = "Niketić, Marjan and Tomović, Gordana and Bokić, Bojana and Buzurović, Uroš and Duraki, Šemija and Đorđević, Vladan and Đurović, Sanja and Krivošej, Zoran and Lazarević, Predrag and Perić, Ranko and Prodanović, Danijela and Radak, Boris and Rat, Milica and Ranimirović, Milana and Stevanović, Vladimir",
year = "2021",
abstract = "The paper proposes eight new nomenclatural combinations and indicates two 
new synonyms. There are 11 taxa (species and subspecies) of the vascular flora 
that are new for the flora of Serbia (of which nine are autochthonous and two 
allochthonous plants). Two autochthonous species were confirmed for the flora of 
Serbia. Six plant taxa are a novelty for proper Serbia, Vojvodina, or Kosovo and 
Metohija. There are eight taxa whose presence in Serbia or in its territorial units has
been refuted., У раду је предложено осам нових номенклатурних комбинација и 
назначена су два нова синонима. Поред тога, представљено је 11
таксона (врста и подврста) васкуларне флоре који су нови за флору 
Србије (од тога девет аутохтоних и две алохтоне биљке). Потврђено је 
и присуство две васкуларне биљке за флору Србије. Шест биљних 
таксона представљају новину за ужу Србију, Војводину или Косово и 
Метохију. Такође је присуство неких таксона у Србији или у њеним 
територијалним јединицама оповргнуто.",
publisher = "Natural History Museum, Belgrade, Serbia",
journal = "Bulletin of the Natural History Museum",
title = "Material on the Annotated Checklist of Vascular Flora of Serbia. Nomenclatural, taxonomic and floristic notes III, Материјал за kритичку листу врста васкуларне флоре Србије. номенклатурни, таксономски и флористички прилози III",
volume = "14",
pages = "77-132",
doi = "10.5937/bnhmb2114077N"
}

Niketić, M., Tomović, G., Bokić, B., Buzurović, U., Duraki, Š., Đorđević, V., Đurović, S., Krivošej, Z., Lazarević, P., Perić, R., Prodanović, D., Radak, B., Rat, M., Ranimirović, M.,& Stevanović, V.. (2021). Material on the Annotated Checklist of Vascular Flora of Serbia. Nomenclatural, taxonomic and floristic notes III. in Bulletin of the Natural History Museum
Natural History Museum, Belgrade, Serbia., 14, 77-132.
https://doi.org/10.5937/bnhmb2114077N

Niketić M, Tomović G, Bokić B, Buzurović U, Duraki Š, Đorđević V, Đurović S, Krivošej Z, Lazarević P, Perić R, Prodanović D, Radak B, Rat M, Ranimirović M, Stevanović V. Material on the Annotated Checklist of Vascular Flora of Serbia. Nomenclatural, taxonomic and floristic notes III. in Bulletin of the Natural History Museum. 2021;14:77-132.
doi:10.5937/bnhmb2114077N .

Niketić, Marjan, Tomović, Gordana, Bokić, Bojana, Buzurović, Uroš, Duraki, Šemija, Đorđević, Vladan, Đurović, Sanja, Krivošej, Zoran, Lazarević, Predrag, Perić, Ranko, Prodanović, Danijela, Radak, Boris, Rat, Milica, Ranimirović, Milana, Stevanović, Vladimir, "Material on the Annotated Checklist of Vascular Flora of Serbia. Nomenclatural, taxonomic and floristic notes III" in Bulletin of the Natural History Museum, 14 (2021):77-132,
https://doi.org/10.5937/bnhmb2114077N . .

TY  - JOUR
AU  - Đoković, Jelena
AU  - Savić, Sanela
AU  - Mitrović, Jelena
AU  - Nikolić, Ines
AU  - Marković, Bojan
AU  - Ranđelović, Danijela
AU  - Antić-Stanković, Jelena
AU  - Božić, Dragana
AU  - Cekić, Nebojša
AU  - Stevanović, Vladimir
AU  - Batinić, Bojan
AU  - Aranđelović, Jovana
AU  - Savić, Miroslav
AU  - Savić, Snežana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5541
AB  - The current study describes the experimental design guided development of PEGylated nanoemulsions as parenteral delivery systems for curcumin, a powerful antioxidant, as well as the evaluation of their physicochemical characteristics and antioxidant activity during the two years of storage. Experimental design setup helped development of nanoemulsion templates with critical quality attributes in line with parenteral application route. Curcumin-loaded nanoemulsions showed mean droplet size about 105 nm, polydispersity index <0.15, zeta potential of −40 mV, and acceptable osmolality of about 550 mOsm/kg. After two years of storage at room temperature, all formulations remained stable. Moreover, antioxidant activity remained intact, as demonstrated by DPPH (IC50 values 0.078–0.075 mg/mL after two years) and FRAPS assays. In vitro release testing proved that PEGylated phospholipids slowed down the curcumin release from nanoemulsions. The nanoemulsion carrier has been proven safe by the MTT test conducted with MRC-5 cell line, and effective on LS cell line. Results from the pharmacokinetic pilot study implied the PEGylated nanoemulsions improved plasma residence of curcumin 20 min after intravenous administration, compared to the non-PEGylated nanoemulsion (two-fold higher) or curcumin solution (three-fold higher). Overall, conclusion suggests that developed PEGylated nanoemulsions present an acceptable delivery system for parenteral administration of curcumin, being effective in preserving its stability and antioxidant capacity at the level highly comparable to the initial findings.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats
VL  - 22
IS  - 15
DO  - 10.3390/ijms22157991
ER  - 

@article{
author = "Đoković, Jelena and Savić, Sanela and Mitrović, Jelena and Nikolić, Ines and Marković, Bojan and Ranđelović, Danijela and Antić-Stanković, Jelena and Božić, Dragana and Cekić, Nebojša and Stevanović, Vladimir and Batinić, Bojan and Aranđelović, Jovana and Savić, Miroslav and Savić, Snežana",
year = "2021",
abstract = "The current study describes the experimental design guided development of PEGylated nanoemulsions as parenteral delivery systems for curcumin, a powerful antioxidant, as well as the evaluation of their physicochemical characteristics and antioxidant activity during the two years of storage. Experimental design setup helped development of nanoemulsion templates with critical quality attributes in line with parenteral application route. Curcumin-loaded nanoemulsions showed mean droplet size about 105 nm, polydispersity index <0.15, zeta potential of −40 mV, and acceptable osmolality of about 550 mOsm/kg. After two years of storage at room temperature, all formulations remained stable. Moreover, antioxidant activity remained intact, as demonstrated by DPPH (IC50 values 0.078–0.075 mg/mL after two years) and FRAPS assays. In vitro release testing proved that PEGylated phospholipids slowed down the curcumin release from nanoemulsions. The nanoemulsion carrier has been proven safe by the MTT test conducted with MRC-5 cell line, and effective on LS cell line. Results from the pharmacokinetic pilot study implied the PEGylated nanoemulsions improved plasma residence of curcumin 20 min after intravenous administration, compared to the non-PEGylated nanoemulsion (two-fold higher) or curcumin solution (three-fold higher). Overall, conclusion suggests that developed PEGylated nanoemulsions present an acceptable delivery system for parenteral administration of curcumin, being effective in preserving its stability and antioxidant capacity at the level highly comparable to the initial findings.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats",
volume = "22",
number = "15",
doi = "10.3390/ijms22157991"
}

Đoković, J., Savić, S., Mitrović, J., Nikolić, I., Marković, B., Ranđelović, D., Antić-Stanković, J., Božić, D., Cekić, N., Stevanović, V., Batinić, B., Aranđelović, J., Savić, M.,& Savić, S.. (2021). Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats. in International Journal of Molecular Sciences
MDPI., 22(15).
https://doi.org/10.3390/ijms22157991

Đoković J, Savić S, Mitrović J, Nikolić I, Marković B, Ranđelović D, Antić-Stanković J, Božić D, Cekić N, Stevanović V, Batinić B, Aranđelović J, Savić M, Savić S. Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats. in International Journal of Molecular Sciences. 2021;22(15).
doi:10.3390/ijms22157991 .

Đoković, Jelena, Savić, Sanela, Mitrović, Jelena, Nikolić, Ines, Marković, Bojan, Ranđelović, Danijela, Antić-Stanković, Jelena, Božić, Dragana, Cekić, Nebojša, Stevanović, Vladimir, Batinić, Bojan, Aranđelović, Jovana, Savić, Miroslav, Savić, Snežana, "Curcumin loaded pegylated nanoemulsions designed for maintained antioxidant effects and improved bioavailability: A pilot study on rats" in International Journal of Molecular Sciences, 22, no. 15 (2021),
https://doi.org/10.3390/ijms22157991 . .

TY  - CONF
AU  - Niketić, Marjan
AU  - Petrović, Silvana
AU  - Pavlović, Milica
AU  - Stevanović, Vladimir
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5164
AB  - The genus Cerastium L., with ca. 200 species primarily distributed in the Palaearctic, is one of the
largest genus in the family Caryophyllaceae. The type subsection of the genus (subsect. Cerastium) encompasses between 55 and 60 perennial species. The chorological analyses had shown that the present
centres of diversity and endemism both of genus (34 species) and the type subsection (19 species) are
located in the area of Balkan countries.
Giving that subsect. Cerastium represents an intricate and highly variable taxonomic group, we performed a HPLC analysis of phenolic compounds (flavonoids and phenolic acids) in methanol extracts of the flowering herbs of 15 taxa (including 9 species) from 17 Balkan localities in order to elucidate their chemotaxonomic differentiation. The HPLC chromatograms of 17 samples showed presence of 43 compounds grouped into 5 classes: O-glycosides of apigenin, C-glycosides of apigenin and luteolin, flavonoid aglycones and phenolic acids. Among the mentioned phenolic compounds we have identified: apigenin 7-O-glucoside, apigenin 7-O-rutinoside, vitexin, vitexin 2"-O-ramnoside, homoorientine and free apigenin. Apigenin O- and C-glycosides were detected in all samples. None of the compounds or compound groups has a statistically important impact on subspecies differentiation.
Among the studied taxa, C. dinaricum G. Beck & Szysz. particularly stands out, as it differs by a significantly smaller number of compounds and the lack of C-glycosides of luteolin. The second isolated
group includes C. banaticum (Rochel) Steudel and C. arvense L., characterized by presence of phenolic acids, which were not registered in other samples. The third (C. alpinum L., C. malyi (Georgiev) Niketić
and C. eriophorum Kit.) and the fourth group (C. decalvans Schlosser & Vuk. , C. grandiflorum Waldst. & Kit. and C. candidissimum Correns) do not show great differences in composition of compounds.
C3  - 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts
T1  - Chemosystematic investigations on phenolic compounds of some Cerastium taxa (Caryophyllaceae) from Balkan Peninsula
SP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5164
ER  - 

@conference{
author = "Niketić, Marjan and Petrović, Silvana and Pavlović, Milica and Stevanović, Vladimir",
year = "2009",
abstract = "The genus Cerastium L., with ca. 200 species primarily distributed in the Palaearctic, is one of the
largest genus in the family Caryophyllaceae. The type subsection of the genus (subsect. Cerastium) encompasses between 55 and 60 perennial species. The chorological analyses had shown that the present
centres of diversity and endemism both of genus (34 species) and the type subsection (19 species) are
located in the area of Balkan countries.
Giving that subsect. Cerastium represents an intricate and highly variable taxonomic group, we performed a HPLC analysis of phenolic compounds (flavonoids and phenolic acids) in methanol extracts of the flowering herbs of 15 taxa (including 9 species) from 17 Balkan localities in order to elucidate their chemotaxonomic differentiation. The HPLC chromatograms of 17 samples showed presence of 43 compounds grouped into 5 classes: O-glycosides of apigenin, C-glycosides of apigenin and luteolin, flavonoid aglycones and phenolic acids. Among the mentioned phenolic compounds we have identified: apigenin 7-O-glucoside, apigenin 7-O-rutinoside, vitexin, vitexin 2"-O-ramnoside, homoorientine and free apigenin. Apigenin O- and C-glycosides were detected in all samples. None of the compounds or compound groups has a statistically important impact on subspecies differentiation.
Among the studied taxa, C. dinaricum G. Beck & Szysz. particularly stands out, as it differs by a significantly smaller number of compounds and the lack of C-glycosides of luteolin. The second isolated
group includes C. banaticum (Rochel) Steudel and C. arvense L., characterized by presence of phenolic acids, which were not registered in other samples. The third (C. alpinum L., C. malyi (Georgiev) Niketić
and C. eriophorum Kit.) and the fourth group (C. decalvans Schlosser & Vuk. , C. grandiflorum Waldst. & Kit. and C. candidissimum Correns) do not show great differences in composition of compounds.",
journal = "5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts",
title = "Chemosystematic investigations on phenolic compounds of some Cerastium taxa (Caryophyllaceae) from Balkan Peninsula",
pages = "29",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5164"
}

Niketić, M., Petrović, S., Pavlović, M.,& Stevanović, V.. (2009). Chemosystematic investigations on phenolic compounds of some Cerastium taxa (Caryophyllaceae) from Balkan Peninsula. in 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts, 29.
https://hdl.handle.net/21.15107/rcub_farfar_5164

Niketić M, Petrović S, Pavlović M, Stevanović V. Chemosystematic investigations on phenolic compounds of some Cerastium taxa (Caryophyllaceae) from Balkan Peninsula. in 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts. 2009;:29.
https://hdl.handle.net/21.15107/rcub_farfar_5164 .

Niketić, Marjan, Petrović, Silvana, Pavlović, Milica, Stevanović, Vladimir, "Chemosystematic investigations on phenolic compounds of some Cerastium taxa (Caryophyllaceae) from Balkan Peninsula" in 5th Balkan Botanical Congress, 07-11 September 2009, Belgrade, Serbia, Book of Abstracts (2009):29,
https://hdl.handle.net/21.15107/rcub_farfar_5164 .