TY  - JOUR
AU  - Zbiljić, Miloš
AU  - Lakušić, Branislava
AU  - Kuzmanović, Nevena
AU  - Stojanović, Danilo
AU  - Lakušić, Dmitar
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5125
AB  - In this work, the overall morpho-anatomical variability of Teucrium montanum sensu lato on the Balkan Peninsula was described. A total of 60 populations were collected all over the Balkans. For each analysed individual, the morphological characters of the stem, leaves, and flowers, as well as the anatomical characters of leaves were measured. The habitats of each population were characterized by altitude zone, type of geological substrate, vegetation, and climate type. Basic descriptive statistics were calculated for each character state, and multivariate analyses were used to compare all assessed characters and groups. The results showed that the analysed populations exhibit high morphological variability and broad ecological preferences. Five of the seven morphologically defined groups fit the already described taxa (T. montanum subsp. montanum, T. montanum subsp. helianthemoides, T. pannonicum, T. skorpili, T. montanum var. parnassicum), whereas two groups are morphologically specific but did not fit the existing taxonomic concepts. The patterns of observed morphological variability only partially match the patterns of ecological differentiation of the habitats of the analysed populations. The occurrence of different morphological groups inhabiting similar habitats in the same biogeographic region suggests that the morpho-anatomical differentiation of T. montanum s.l. may be due to genetic or evolutionary factors.
PB  - Taylor and Francis
T2  - Plant Biosystems
T1  - Morphological diversification of Teucrium montanum sensu lato on the  Balkan Peninsula
VL  - 157
IS  - 3
SP  - 670
EP  - 687
DO  - 10.1080/11263504.2023.2186509
ER  - 

@article{
author = "Zbiljić, Miloš and Lakušić, Branislava and Kuzmanović, Nevena and Stojanović, Danilo and Lakušić, Dmitar",
year = "2023",
abstract = "In this work, the overall morpho-anatomical variability of Teucrium montanum sensu lato on the Balkan Peninsula was described. A total of 60 populations were collected all over the Balkans. For each analysed individual, the morphological characters of the stem, leaves, and flowers, as well as the anatomical characters of leaves were measured. The habitats of each population were characterized by altitude zone, type of geological substrate, vegetation, and climate type. Basic descriptive statistics were calculated for each character state, and multivariate analyses were used to compare all assessed characters and groups. The results showed that the analysed populations exhibit high morphological variability and broad ecological preferences. Five of the seven morphologically defined groups fit the already described taxa (T. montanum subsp. montanum, T. montanum subsp. helianthemoides, T. pannonicum, T. skorpili, T. montanum var. parnassicum), whereas two groups are morphologically specific but did not fit the existing taxonomic concepts. The patterns of observed morphological variability only partially match the patterns of ecological differentiation of the habitats of the analysed populations. The occurrence of different morphological groups inhabiting similar habitats in the same biogeographic region suggests that the morpho-anatomical differentiation of T. montanum s.l. may be due to genetic or evolutionary factors.",
publisher = "Taylor and Francis",
journal = "Plant Biosystems",
title = "Morphological diversification of Teucrium montanum sensu lato on the  Balkan Peninsula",
volume = "157",
number = "3",
pages = "670-687",
doi = "10.1080/11263504.2023.2186509"
}

Zbiljić, M., Lakušić, B., Kuzmanović, N., Stojanović, D.,& Lakušić, D.. (2023). Morphological diversification of Teucrium montanum sensu lato on the  Balkan Peninsula. in Plant Biosystems
Taylor and Francis., 157(3), 670-687.
https://doi.org/10.1080/11263504.2023.2186509

Zbiljić M, Lakušić B, Kuzmanović N, Stojanović D, Lakušić D. Morphological diversification of Teucrium montanum sensu lato on the  Balkan Peninsula. in Plant Biosystems. 2023;157(3):670-687.
doi:10.1080/11263504.2023.2186509 .

Zbiljić, Miloš, Lakušić, Branislava, Kuzmanović, Nevena, Stojanović, Danilo, Lakušić, Dmitar, "Morphological diversification of Teucrium montanum sensu lato on the  Balkan Peninsula" in Plant Biosystems, 157, no. 3 (2023):670-687,
https://doi.org/10.1080/11263504.2023.2186509 . .

TY  - JOUR
AU  - Niketić, Marjan
AU  - Tomović, Gordana
AU  - Anačkov, Goran
AU  - Đorđević, Vladan
AU  - Đurović, Sanja
AU  - Duraki, Šemija
AU  - Kabaš, Eva
AU  - Lakušić, Dmitar
AU  - Petkovski, Gorana
AU  - Petrović, Silvana
AU  - Ranimirović, Milana
AU  - Slavkovska, Violeta
AU  - Ušjak, Ljuboš
AU  - Zbiljić, Miloš
AU  - Zlatković, Bojan
AU  - Stevanović, Vladimir
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4958
AB  - This paper represents the fourth part of the inventory of the flora of Serbia (Niketić et al. 2018, 2020, 2021), which contains nomenclatural, taxonomic and floristic notes related to taxa from the Magnoliopsida group. At the same time, this contribution is the basis for publication of subsequent volumes of the An annotated checklist of vascular flora of Serbia in order to supplement the data on vascular plants in our country.
AB  - У раду је предложено пет нових номенклатурних комбинација, а поред тога, представљено је 17 таксона (врста, подврста и хибрида) васкуларне флоре који су нови за флору Србије (од тога 12 аутохтоних и пет алохтоних биљак). Потврђени су стари или непоуздани наводи за две аутохтоне биљке у флори Србије. У последњем делу рада дате су информације о деветнаест таксона чије је присуство у Србији или у њеним територијалним јединицама оповргнуто.
PB  - Natural History Museum, Belgrade, Serbia
T2  - Bulletin of the Natural History Museum
T1  - Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV
T1  - Mатеријал за Kритичку листу врста васкуларне флоре Србије. Номенклатурни, таксономски и флористички прилози IV
VL  - 15
SP  - 27
EP  - 96
DO  - 10.5937/bnhmb2215027N
ER  - 

@article{
author = "Niketić, Marjan and Tomović, Gordana and Anačkov, Goran and Đorđević, Vladan and Đurović, Sanja and Duraki, Šemija and Kabaš, Eva and Lakušić, Dmitar and Petkovski, Gorana and Petrović, Silvana and Ranimirović, Milana and Slavkovska, Violeta and Ušjak, Ljuboš and Zbiljić, Miloš and Zlatković, Bojan and Stevanović, Vladimir",
year = "2022",
abstract = "This paper represents the fourth part of the inventory of the flora of Serbia (Niketić et al. 2018, 2020, 2021), which contains nomenclatural, taxonomic and floristic notes related to taxa from the Magnoliopsida group. At the same time, this contribution is the basis for publication of subsequent volumes of the An annotated checklist of vascular flora of Serbia in order to supplement the data on vascular plants in our country., У раду је предложено пет нових номенклатурних комбинација, а поред тога, представљено је 17 таксона (врста, подврста и хибрида) васкуларне флоре који су нови за флору Србије (од тога 12 аутохтоних и пет алохтоних биљак). Потврђени су стари или непоуздани наводи за две аутохтоне биљке у флори Србије. У последњем делу рада дате су информације о деветнаест таксона чије је присуство у Србији или у њеним територијалним јединицама оповргнуто.",
publisher = "Natural History Museum, Belgrade, Serbia",
journal = "Bulletin of the Natural History Museum",
title = "Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV, Mатеријал за Kритичку листу врста васкуларне флоре Србије. Номенклатурни, таксономски и флористички прилози IV",
volume = "15",
pages = "27-96",
doi = "10.5937/bnhmb2215027N"
}

Niketić, M., Tomović, G., Anačkov, G., Đorđević, V., Đurović, S., Duraki, Š., Kabaš, E., Lakušić, D., Petkovski, G., Petrović, S., Ranimirović, M., Slavkovska, V., Ušjak, L., Zbiljić, M., Zlatković, B.,& Stevanović, V.. (2022). Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV. in Bulletin of the Natural History Museum
Natural History Museum, Belgrade, Serbia., 15, 27-96.
https://doi.org/10.5937/bnhmb2215027N

Niketić M, Tomović G, Anačkov G, Đorđević V, Đurović S, Duraki Š, Kabaš E, Lakušić D, Petkovski G, Petrović S, Ranimirović M, Slavkovska V, Ušjak L, Zbiljić M, Zlatković B, Stevanović V. Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV. in Bulletin of the Natural History Museum. 2022;15:27-96.
doi:10.5937/bnhmb2215027N .

Niketić, Marjan, Tomović, Gordana, Anačkov, Goran, Đorđević, Vladan, Đurović, Sanja, Duraki, Šemija, Kabaš, Eva, Lakušić, Dmitar, Petkovski, Gorana, Petrović, Silvana, Ranimirović, Milana, Slavkovska, Violeta, Ušjak, Ljuboš, Zbiljić, Miloš, Zlatković, Bojan, Stevanović, Vladimir, "Material on the Annotated checklist of vascular flora of Serbia. Nomenclatural, taxonomic and floristic notes IV" in Bulletin of the Natural History Museum, 15 (2022):27-96,
https://doi.org/10.5937/bnhmb2215027N . .

TY  - CONF
AU  - Zbiljić, Miloš
AU  - Lakušić, Branislava
AU  - Kuzmanović, Nevena
AU  - Stojanović, Danilo
AU  - Lakušić, Dmitar
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5147
AB  - Teucrium montanum L. is a semi-woody evergreen small shrub distributed in southern and central Europe, Asia Minor, and Algeria in North Africa. The species has a very wide ecological valence occurring in various Mediterranean, sub-Mediterranean, continental as well as oro-Mediterranean and Alpine habitats. Due to its wide ecological valence and broad distribution, T. montanum displays high morphological variability, which is reflected in a large number of described taxa associated with it. In Europe, about thirty taxa have been described at specific and infraspecific levels, of which only T. montanum subsp. montanum and T. montanum subsp. helianthemoides (Adamović) Baden are accepted, while all the others are considered synonyms of T. montanum. The observed morphological diversity is reflected in the presence of six taxa described from the Balkan Peninsula: T. montanum subsp. helianthemoides, T. montanum var. hirsutum Boiss., T. montanum var. modestum Heldr., T. montanum var. parnassicum Čelak., T. pannonicum A. Kern., and T. skorpili Velen. During field research throughout the Balkans, seven morphologically more or less well-defined groups with relatively solid geographical differentiation were observed. Five of the seven groups fit the already described infraspecific taxa, while two groups are morphologically specific but do not correspond to the existing taxa. The aim of this study was to describe the overall morpho-anatomical variability of T. montanum sensu lato in the Balkans and to determine whether the seven identified groups can be distinguished based on morpho-anatomical characters. The most typical populations of these groups could be separated quite well based on the quantitative morphological traits. Multivariate analysis of all samples showed a solid separation of three groups and a significant number of individuals with intermediate positions. Other groups did not show statistical diversification but possess a specific combination of morphological characters by which they can be identified.
PB  - Hrvatsko botaničko društvo (Croatian Botanical Society)
C3  - 7th Croatian Botanical Symposium with international participation, Zagreb 2022 - Book of Abstracts
T1  - Morphological diversification of Teucrium montanum L. sensu lato on the Balkan Peninsula
SP  - 33
EP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5147
ER  - 

@conference{
author = "Zbiljić, Miloš and Lakušić, Branislava and Kuzmanović, Nevena and Stojanović, Danilo and Lakušić, Dmitar",
year = "2022",
abstract = "Teucrium montanum L. is a semi-woody evergreen small shrub distributed in southern and central Europe, Asia Minor, and Algeria in North Africa. The species has a very wide ecological valence occurring in various Mediterranean, sub-Mediterranean, continental as well as oro-Mediterranean and Alpine habitats. Due to its wide ecological valence and broad distribution, T. montanum displays high morphological variability, which is reflected in a large number of described taxa associated with it. In Europe, about thirty taxa have been described at specific and infraspecific levels, of which only T. montanum subsp. montanum and T. montanum subsp. helianthemoides (Adamović) Baden are accepted, while all the others are considered synonyms of T. montanum. The observed morphological diversity is reflected in the presence of six taxa described from the Balkan Peninsula: T. montanum subsp. helianthemoides, T. montanum var. hirsutum Boiss., T. montanum var. modestum Heldr., T. montanum var. parnassicum Čelak., T. pannonicum A. Kern., and T. skorpili Velen. During field research throughout the Balkans, seven morphologically more or less well-defined groups with relatively solid geographical differentiation were observed. Five of the seven groups fit the already described infraspecific taxa, while two groups are morphologically specific but do not correspond to the existing taxa. The aim of this study was to describe the overall morpho-anatomical variability of T. montanum sensu lato in the Balkans and to determine whether the seven identified groups can be distinguished based on morpho-anatomical characters. The most typical populations of these groups could be separated quite well based on the quantitative morphological traits. Multivariate analysis of all samples showed a solid separation of three groups and a significant number of individuals with intermediate positions. Other groups did not show statistical diversification but possess a specific combination of morphological characters by which they can be identified.",
publisher = "Hrvatsko botaničko društvo (Croatian Botanical Society)",
journal = "7th Croatian Botanical Symposium with international participation, Zagreb 2022 - Book of Abstracts",
title = "Morphological diversification of Teucrium montanum L. sensu lato on the Balkan Peninsula",
pages = "33-33",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5147"
}

Zbiljić, M., Lakušić, B., Kuzmanović, N., Stojanović, D.,& Lakušić, D.. (2022). Morphological diversification of Teucrium montanum L. sensu lato on the Balkan Peninsula. in 7th Croatian Botanical Symposium with international participation, Zagreb 2022 - Book of Abstracts
Hrvatsko botaničko društvo (Croatian Botanical Society)., 33-33.
https://hdl.handle.net/21.15107/rcub_farfar_5147

Zbiljić M, Lakušić B, Kuzmanović N, Stojanović D, Lakušić D. Morphological diversification of Teucrium montanum L. sensu lato on the Balkan Peninsula. in 7th Croatian Botanical Symposium with international participation, Zagreb 2022 - Book of Abstracts. 2022;:33-33.
https://hdl.handle.net/21.15107/rcub_farfar_5147 .

Zbiljić, Miloš, Lakušić, Branislava, Kuzmanović, Nevena, Stojanović, Danilo, Lakušić, Dmitar, "Morphological diversification of Teucrium montanum L. sensu lato on the Balkan Peninsula" in 7th Croatian Botanical Symposium with international participation, Zagreb 2022 - Book of Abstracts (2022):33-33,
https://hdl.handle.net/21.15107/rcub_farfar_5147 .

TY  - JOUR
AU  - Zbiljić, Miloš
AU  - Lakušić, Dmitar
AU  - Stevanoski, Ivana
AU  - Kuzmanović, Nevena
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5128
AB  - Teucrium montanum is a species widely distributed in Europe, occurring also in North Africa and Asia Minor. Because of its wide distribution and high morphological variability, many taxa related to T. montanum have been described. In this paper we discuss and lectotypify the following six names of taxa described or reported for the Balkan Peninsula: T. helianthemoides, T. montanum var. hirsutum, T. montanum var. modestum, T. montanum var. parnassicum, T. pannonicum, and T. skorpilii.
PB  - Magnolia Press
T2  - Phytotaxa
T1  - Lectotypification of names related to Teucrium montanum L. (Lamiaceae) reported  for the Balkan peninsula
VL  - 530
IS  - 2
SP  - 198
EP  - 204
DO  - 10.11646/phytotaxa.530.2.6
ER  - 

@article{
author = "Zbiljić, Miloš and Lakušić, Dmitar and Stevanoski, Ivana and Kuzmanović, Nevena",
year = "2022",
abstract = "Teucrium montanum is a species widely distributed in Europe, occurring also in North Africa and Asia Minor. Because of its wide distribution and high morphological variability, many taxa related to T. montanum have been described. In this paper we discuss and lectotypify the following six names of taxa described or reported for the Balkan Peninsula: T. helianthemoides, T. montanum var. hirsutum, T. montanum var. modestum, T. montanum var. parnassicum, T. pannonicum, and T. skorpilii.",
publisher = "Magnolia Press",
journal = "Phytotaxa",
title = "Lectotypification of names related to Teucrium montanum L. (Lamiaceae) reported  for the Balkan peninsula",
volume = "530",
number = "2",
pages = "198-204",
doi = "10.11646/phytotaxa.530.2.6"
}

Zbiljić, M., Lakušić, D., Stevanoski, I.,& Kuzmanović, N.. (2022). Lectotypification of names related to Teucrium montanum L. (Lamiaceae) reported  for the Balkan peninsula. in Phytotaxa
Magnolia Press., 530(2), 198-204.
https://doi.org/10.11646/phytotaxa.530.2.6

Zbiljić M, Lakušić D, Stevanoski I, Kuzmanović N. Lectotypification of names related to Teucrium montanum L. (Lamiaceae) reported  for the Balkan peninsula. in Phytotaxa. 2022;530(2):198-204.
doi:10.11646/phytotaxa.530.2.6 .

Zbiljić, Miloš, Lakušić, Dmitar, Stevanoski, Ivana, Kuzmanović, Nevena, "Lectotypification of names related to Teucrium montanum L. (Lamiaceae) reported  for the Balkan peninsula" in Phytotaxa, 530, no. 2 (2022):198-204,
https://doi.org/10.11646/phytotaxa.530.2.6 . .

TY  - JOUR
AU  - Jovanović, Maja
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
AU  - Zlatković, Bojan
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3999
AB  - Several related yellow-flowered houseleek species which occur on the Balkan Peninsula are divided into two complexes: Sempervivum ciliosum (S. ciliosum, S. jakucsii, S. klepa, S. octopodes, and S. galicicum) and the S. ruthenicum complex (S. ruthenicum, S. leucanthum, S. kindingeri, and S. zeleborii). Due to strong phenotypic plasticity and a limited number of studies, it is difficult to assert at this point whether all the above species are well defined in the taxonomic sense. Detailed studies of the epidermal structures have not been conducted for any of the species in either complex. The aim of this study was to investigate the degree of variability of the epidermal structures together with their potential usefulness for the taxonomic characterization of the species studied. A total of 18 quantitative characters of the epidermal structures of the adaxial and abaxial surfaces of the rosette leaves were analysed within 16 populations. In all species, the epidermal cells are polygonal or irregularly shaped, with straight or sinuous anticlinal walls, while the rosette leaves are amphistomatic with anisocytic stomata. Simple biseriate multicellular glandular trichomes were found on the adaxial and abaxial surfaces and the margins of the rosette leaves. The results of the descriptive statistics, univariate (ANOVA) and multivariate statistical analysis (CDA, AHC) showed low to high variability in the epidermal cells, guard cells and trichomes. The multivariate analysis showed diversification among the complexes and species. The length of the marginal and apical trichomes of the rosette leaves contributed most to diversification.
AB  - Balkansko poluostrvo nastanjuje nekoliko sličnih, žutocvetnih vrsta čuvarkuća svrstanih u dva kompleksa: Sempervivum ciliosum (S. ciliosum, S. jakucsii, S. klepa, S. octopodes, S. galicicum) i S. ruthenicum (S. ruthenicum, S. leucanthum, S. kindingeri, S. zeleborii). Usled izrazite fenotipske plastičnosti trenutno je teško utvrditi da li su sve vrste pomenutih kompleksa dobro definisane u taksonomskom smislu. Detaljna istraživanja epidermalnih struktura do sada nisu sprovedena ni za jednu od navedenih vrsta. Cilj ove studije bio je da se ispita stepen varijabilnosti epidermalnih struktura i mogućnost njihove primene u taksonomskoj karakterizaciji navedenih vrsta. Ukupno 18 kvantitativnih osobina epidermalnih struktura adaksijalne i abaksijalne površine listova rozete je analizirano na nivou 16 populacija. Kod svih vrsta, epidermalne ćelije su poligonalnog ili nepravilnog oblika, sa ravnim ili vijugavim antiklinalnim zidovima, dok su listovi amfistomatični sa anizocitnim tipom stoma. Prosti biserijatni višećelijski žlezdani trihomi pronađeni su na adaksijalnoj i abaksijalnoj površini, kao i na marginama listova rozete. Rezultati deskriptivne statistike, univarijantnih (ANOVA) i multivarijantnih statističkih analiza (CDA, AHC) ukazuju na nizak do visok stepen varijabilnosti epidermalnih ćelija, ćelija zatvaračica i trihoma. Multivarijantne analize ukazuju na raznolikost kompleksa i vrsta, dok dužine marginalnih i apikalnih trihoma listova u najvećoj meri doprinose njihovoj različitosti.
PB  - Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade
T2  - Botanica Serbica
T1  - Diversification of yellow-flowered Sempervivum (Crassulaceae) species from the Balkan Peninsula: evidence from the morphometric study of the epidermal structures of rosette leaves
T1  - Različitost žutocvetnih vrsta roda Sempervivum (Crassulaceae) Balkanskog poluostrva: rezultati morfometrijskog istraživanja epidermalnih struktura listova rozete.
VL  - 45
IS  - 2
SP  - 163
EP  - 176
DO  - 10.2298/BOTSERB2102163J
ER  - 

@article{
author = "Jovanović, Maja and Lakušić, Dmitar and Lakušić, Branislava and Zlatković, Bojan",
year = "2021",
abstract = "Several related yellow-flowered houseleek species which occur on the Balkan Peninsula are divided into two complexes: Sempervivum ciliosum (S. ciliosum, S. jakucsii, S. klepa, S. octopodes, and S. galicicum) and the S. ruthenicum complex (S. ruthenicum, S. leucanthum, S. kindingeri, and S. zeleborii). Due to strong phenotypic plasticity and a limited number of studies, it is difficult to assert at this point whether all the above species are well defined in the taxonomic sense. Detailed studies of the epidermal structures have not been conducted for any of the species in either complex. The aim of this study was to investigate the degree of variability of the epidermal structures together with their potential usefulness for the taxonomic characterization of the species studied. A total of 18 quantitative characters of the epidermal structures of the adaxial and abaxial surfaces of the rosette leaves were analysed within 16 populations. In all species, the epidermal cells are polygonal or irregularly shaped, with straight or sinuous anticlinal walls, while the rosette leaves are amphistomatic with anisocytic stomata. Simple biseriate multicellular glandular trichomes were found on the adaxial and abaxial surfaces and the margins of the rosette leaves. The results of the descriptive statistics, univariate (ANOVA) and multivariate statistical analysis (CDA, AHC) showed low to high variability in the epidermal cells, guard cells and trichomes. The multivariate analysis showed diversification among the complexes and species. The length of the marginal and apical trichomes of the rosette leaves contributed most to diversification., Balkansko poluostrvo nastanjuje nekoliko sličnih, žutocvetnih vrsta čuvarkuća svrstanih u dva kompleksa: Sempervivum ciliosum (S. ciliosum, S. jakucsii, S. klepa, S. octopodes, S. galicicum) i S. ruthenicum (S. ruthenicum, S. leucanthum, S. kindingeri, S. zeleborii). Usled izrazite fenotipske plastičnosti trenutno je teško utvrditi da li su sve vrste pomenutih kompleksa dobro definisane u taksonomskom smislu. Detaljna istraživanja epidermalnih struktura do sada nisu sprovedena ni za jednu od navedenih vrsta. Cilj ove studije bio je da se ispita stepen varijabilnosti epidermalnih struktura i mogućnost njihove primene u taksonomskoj karakterizaciji navedenih vrsta. Ukupno 18 kvantitativnih osobina epidermalnih struktura adaksijalne i abaksijalne površine listova rozete je analizirano na nivou 16 populacija. Kod svih vrsta, epidermalne ćelije su poligonalnog ili nepravilnog oblika, sa ravnim ili vijugavim antiklinalnim zidovima, dok su listovi amfistomatični sa anizocitnim tipom stoma. Prosti biserijatni višećelijski žlezdani trihomi pronađeni su na adaksijalnoj i abaksijalnoj površini, kao i na marginama listova rozete. Rezultati deskriptivne statistike, univarijantnih (ANOVA) i multivarijantnih statističkih analiza (CDA, AHC) ukazuju na nizak do visok stepen varijabilnosti epidermalnih ćelija, ćelija zatvaračica i trihoma. Multivarijantne analize ukazuju na raznolikost kompleksa i vrsta, dok dužine marginalnih i apikalnih trihoma listova u najvećoj meri doprinose njihovoj različitosti.",
publisher = "Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade",
journal = "Botanica Serbica",
title = "Diversification of yellow-flowered Sempervivum (Crassulaceae) species from the Balkan Peninsula: evidence from the morphometric study of the epidermal structures of rosette leaves, Različitost žutocvetnih vrsta roda Sempervivum (Crassulaceae) Balkanskog poluostrva: rezultati morfometrijskog istraživanja epidermalnih struktura listova rozete.",
volume = "45",
number = "2",
pages = "163-176",
doi = "10.2298/BOTSERB2102163J"
}

Jovanović, M., Lakušić, D., Lakušić, B.,& Zlatković, B.. (2021). Diversification of yellow-flowered Sempervivum (Crassulaceae) species from the Balkan Peninsula: evidence from the morphometric study of the epidermal structures of rosette leaves. in Botanica Serbica
Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade., 45(2), 163-176.
https://doi.org/10.2298/BOTSERB2102163J

Jovanović M, Lakušić D, Lakušić B, Zlatković B. Diversification of yellow-flowered Sempervivum (Crassulaceae) species from the Balkan Peninsula: evidence from the morphometric study of the epidermal structures of rosette leaves. in Botanica Serbica. 2021;45(2):163-176.
doi:10.2298/BOTSERB2102163J .

Jovanović, Maja, Lakušić, Dmitar, Lakušić, Branislava, Zlatković, Bojan, "Diversification of yellow-flowered Sempervivum (Crassulaceae) species from the Balkan Peninsula: evidence from the morphometric study of the epidermal structures of rosette leaves" in Botanica Serbica, 45, no. 2 (2021):163-176,
https://doi.org/10.2298/BOTSERB2102163J . .

TY  - JOUR
AU  - Zbiljić, Miloš
AU  - Lakušić, Branislava
AU  - Marčetić, Mirjana
AU  - Bogdanović, Sandro
AU  - Lakušić, Dmitar
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3941
AB  - Several natural hybrids of Teucrium montanum L. and T. polium L. have been described in certain parts of the area where these two species are sympatric. A new population with intermediate individuals that share morphological characteristics of both T. montanum and T. polium has been found in Croatia (Bisko near Trilj), and most likely represents a hybridogenous taxon. The aim of this study is to compare the morpho-anatomical and phytochemical characteristics of the two parental species and their putative hybrid, as well as to determine the most important distinguishing characters between these three groups. In order to describe the variability and significance of morpho-anatomical and phytochemical differentiation of the analysed groups several multivariate statistical analysis were conducted (PCA, CDA, DFA, UPGMA-clustering). All analyses confirm the separation of two species, T. polium and T. montanum, and reveal the intermediate position of the putative hybrid. In this way, data on morphological and anatomical analysis together with data on the composition of the essential oils support the assumption of the hybridogenous origin of the intermediate individuals. Based on descriptions and original indication of parental species of previously described hybrids T. × castrense Verg., T. × bogoutdinovae Melinkov and T. × rohlenae K.Malý, we found that the intermediate specimens collected in Croatia perfectly correspond to T. × rohlenae, a hybridogenous taxon discovered by Rohlena in the vicinity of Kotor in Montenegro, and thus prove the presence of a new natural hybrid in the flora of Croatia.
PB  - Department of Biology, Faculty of Science, University of Zagreb
T2  - Acta Botanica Croatica
T1  - Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia
VL  - 80
IS  - 1
SP  - 48
EP  - 55
DO  - 10.37427/botcro-2020-033
ER  - 

@article{
author = "Zbiljić, Miloš and Lakušić, Branislava and Marčetić, Mirjana and Bogdanović, Sandro and Lakušić, Dmitar",
year = "2021",
abstract = "Several natural hybrids of Teucrium montanum L. and T. polium L. have been described in certain parts of the area where these two species are sympatric. A new population with intermediate individuals that share morphological characteristics of both T. montanum and T. polium has been found in Croatia (Bisko near Trilj), and most likely represents a hybridogenous taxon. The aim of this study is to compare the morpho-anatomical and phytochemical characteristics of the two parental species and their putative hybrid, as well as to determine the most important distinguishing characters between these three groups. In order to describe the variability and significance of morpho-anatomical and phytochemical differentiation of the analysed groups several multivariate statistical analysis were conducted (PCA, CDA, DFA, UPGMA-clustering). All analyses confirm the separation of two species, T. polium and T. montanum, and reveal the intermediate position of the putative hybrid. In this way, data on morphological and anatomical analysis together with data on the composition of the essential oils support the assumption of the hybridogenous origin of the intermediate individuals. Based on descriptions and original indication of parental species of previously described hybrids T. × castrense Verg., T. × bogoutdinovae Melinkov and T. × rohlenae K.Malý, we found that the intermediate specimens collected in Croatia perfectly correspond to T. × rohlenae, a hybridogenous taxon discovered by Rohlena in the vicinity of Kotor in Montenegro, and thus prove the presence of a new natural hybrid in the flora of Croatia.",
publisher = "Department of Biology, Faculty of Science, University of Zagreb",
journal = "Acta Botanica Croatica",
title = "Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia",
volume = "80",
number = "1",
pages = "48-55",
doi = "10.37427/botcro-2020-033"
}

Zbiljić, M., Lakušić, B., Marčetić, M., Bogdanović, S.,& Lakušić, D.. (2021). Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia. in Acta Botanica Croatica
Department of Biology, Faculty of Science, University of Zagreb., 80(1), 48-55.
https://doi.org/10.37427/botcro-2020-033

Zbiljić M, Lakušić B, Marčetić M, Bogdanović S, Lakušić D. Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia. in Acta Botanica Croatica. 2021;80(1):48-55.
doi:10.37427/botcro-2020-033 .

Zbiljić, Miloš, Lakušić, Branislava, Marčetić, Mirjana, Bogdanović, Sandro, Lakušić, Dmitar, "Morphological and chemical evidence of Teucrium × rohlenae K. Malý (Lamiaceae), a new hybrid in Croatia" in Acta Botanica Croatica, 80, no. 1 (2021):48-55,
https://doi.org/10.37427/botcro-2020-033 . .

TY  - CONF
AU  - Zbiljić, Miloš
AU  - Lakušić, Branislava
AU  - Marčetić, Mirjana
AU  - Bogdanović, Sandro
AU  - Lakušić, Dmitar
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5304
AB  - Teucrium × rohlenae Maly is a natural hybrid between T. montanum and T.
polium. It was found in the surrounding of Kotor (Montenegro) by L. Čelakovski fil.
and reported by J. Rohlena as T montanum × polium in 1922. This hybrid is validly
described by K. Maly in 1951. After the original description, Teucrium × rohlenae
have not been reported in the Balkan Peninsula, but hybrids between T montanum and
T. polium have been cited in Italy (as Teucrium x castrense) and Moldova (as
Teucrium × bogoutdinovae). In sympatric population of T. montanum and T. polium
in the surrounding of Trilj near Split (Croatia), we found intermediate individuals with
potentially hybridogenic origin. The aim of the study was to analyse 44 morphoanatomical
characters of stem, leaf, inflorescens and calyx, and chemical composition
of volatile compounds of parental species and their potential hybrid Teucrium ×
rohlenae. The composition of hexane extracts of aerial parts was determined using
GC-FID and GC-MS methods. A detailed morpho-anatomical analysis has shown that
parental species are well differentiated, while potential hybrids have an intermediate
position. Volatiles of potential hybrid represented both species, main hydrocarbon
compounds were alike volatiles of T. polium while the terpene profile was more
similar to T. montanum terpenes.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence
SP  - 16
EP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5304
ER  - 

@conference{
author = "Zbiljić, Miloš and Lakušić, Branislava and Marčetić, Mirjana and Bogdanović, Sandro and Lakušić, Dmitar",
year = "2019",
abstract = "Teucrium × rohlenae Maly is a natural hybrid between T. montanum and T.
polium. It was found in the surrounding of Kotor (Montenegro) by L. Čelakovski fil.
and reported by J. Rohlena as T montanum × polium in 1922. This hybrid is validly
described by K. Maly in 1951. After the original description, Teucrium × rohlenae
have not been reported in the Balkan Peninsula, but hybrids between T montanum and
T. polium have been cited in Italy (as Teucrium x castrense) and Moldova (as
Teucrium × bogoutdinovae). In sympatric population of T. montanum and T. polium
in the surrounding of Trilj near Split (Croatia), we found intermediate individuals with
potentially hybridogenic origin. The aim of the study was to analyse 44 morphoanatomical
characters of stem, leaf, inflorescens and calyx, and chemical composition
of volatile compounds of parental species and their potential hybrid Teucrium ×
rohlenae. The composition of hexane extracts of aerial parts was determined using
GC-FID and GC-MS methods. A detailed morpho-anatomical analysis has shown that
parental species are well differentiated, while potential hybrids have an intermediate
position. Volatiles of potential hybrid represented both species, main hydrocarbon
compounds were alike volatiles of T. polium while the terpene profile was more
similar to T. montanum terpenes.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence",
pages = "16-16",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5304"
}

Zbiljić, M., Lakušić, B., Marčetić, M., Bogdanović, S.,& Lakušić, D.. (2019). Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 16-16.
https://hdl.handle.net/21.15107/rcub_farfar_5304

Zbiljić M, Lakušić B, Marčetić M, Bogdanović S, Lakušić D. Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:16-16.
https://hdl.handle.net/21.15107/rcub_farfar_5304 .

Zbiljić, Miloš, Lakušić, Branislava, Marčetić, Mirjana, Bogdanović, Sandro, Lakušić, Dmitar, "Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):16-16,
https://hdl.handle.net/21.15107/rcub_farfar_5304 .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Zbiljić, Miloš
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5257
AB  - Teucrium montanum L. is widely distributed in the Balkan
Peninsula, extending from the sea coast to altitudes of over
2100 m a. s. l. with the highest number of occurrences in the
zone between 500 and 1000 m. The aim of the study was to
investigate the chemical composition of essential oil of aerial
parts of T. montanum from 14 different populations from Balkan Peninsula, ten from Serbia (SR-Trešnjica canyon, SR-Brdjanska gorge, SR-Goč, SR-Gornjak gorge, SR-Grza canyon,
SR-Jelašnica, SR-Kopaonik, SR-Maglič, SR-Rtanj, SR-Sićevačka gorge), two from Greece (GR-Olimp, GR-Ossa) and two
from Albania (AL-Deja, AL-Skadar). The essential oils were
obtained by hydrodistillation and qualitative and quantitative analysis was performed by GC-FID and GC-MS. The
aerial parts of T. montanum contained only small amounts
of oil (traces-0.7%), predominantly composed by sesquiterpene compounds (58.0-99.0%). One population from Albania
(Deja), two from Greece (Olimp, Ossa) and two from Serbia
(Sićevačka gorge, Trešnjica canyon) contained more than 90%
of sesquiterpenes. The composition of essential oils was quite
variable and the main compounds in almost all oils were germacrene D (trace-45.5%), sabinene (trace-23.1%), α-pinene
(trace-20.7%), limonene (trace-20.4%), (E)-caryophyllene (2.9-14.5%), γ-cadinene (trace-13.8%) and δ-cadinene
(trace-12.0%). The cluster analysis revealed the separation
of investigated essential oils to five clusters. The first cluster
(AL-Skadar, SR-Brdjanska gorge, SR-Gornjak gorge, SR-Grza canyon, SR-Goč) is characterized by high germacrene D
content (17.6-45.5%), the second (SR-Maglič, SR-Kopaonik,
SR-Sićevačka gorge) by high sesquiterpene alcohol shyobunol content (14.6-55.2%), the third (AL-Deja, SR-Jelašnica)
by high α-bisabolol content (31.2%-43.7%) and shyobunol
(3.2%) in population AL-Deja, the fourth (SR-Rtanj) by high
cis-sesquisabinene hydrate content (42.4%) and the fifth (GR-Olimp, GR-Ossa, SR-Trešnjica canyon) by high germecrene
D-4-ol (5.96%-61.9%), and β-eudesmol (14.9%) and epi-α-cadinol (10.2%) content in GR-Olimp population. Such a high
variability needs more research to define relationships among
populations.
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
C3  - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
T1  - Variability of essential oil of different populations of Teucrium montanum L. (Lamiaceae) from Balkan peninsula
VL  - 42
IS  - 1 supplement
SP  - 133
EP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5257
ER  - 

@conference{
author = "Marčetić, Mirjana and Zbiljić, Miloš and Lakušić, Dmitar and Lakušić, Branislava",
year = "2018",
abstract = "Teucrium montanum L. is widely distributed in the Balkan
Peninsula, extending from the sea coast to altitudes of over
2100 m a. s. l. with the highest number of occurrences in the
zone between 500 and 1000 m. The aim of the study was to
investigate the chemical composition of essential oil of aerial
parts of T. montanum from 14 different populations from Balkan Peninsula, ten from Serbia (SR-Trešnjica canyon, SR-Brdjanska gorge, SR-Goč, SR-Gornjak gorge, SR-Grza canyon,
SR-Jelašnica, SR-Kopaonik, SR-Maglič, SR-Rtanj, SR-Sićevačka gorge), two from Greece (GR-Olimp, GR-Ossa) and two
from Albania (AL-Deja, AL-Skadar). The essential oils were
obtained by hydrodistillation and qualitative and quantitative analysis was performed by GC-FID and GC-MS. The
aerial parts of T. montanum contained only small amounts
of oil (traces-0.7%), predominantly composed by sesquiterpene compounds (58.0-99.0%). One population from Albania
(Deja), two from Greece (Olimp, Ossa) and two from Serbia
(Sićevačka gorge, Trešnjica canyon) contained more than 90%
of sesquiterpenes. The composition of essential oils was quite
variable and the main compounds in almost all oils were germacrene D (trace-45.5%), sabinene (trace-23.1%), α-pinene
(trace-20.7%), limonene (trace-20.4%), (E)-caryophyllene (2.9-14.5%), γ-cadinene (trace-13.8%) and δ-cadinene
(trace-12.0%). The cluster analysis revealed the separation
of investigated essential oils to five clusters. The first cluster
(AL-Skadar, SR-Brdjanska gorge, SR-Gornjak gorge, SR-Grza canyon, SR-Goč) is characterized by high germacrene D
content (17.6-45.5%), the second (SR-Maglič, SR-Kopaonik,
SR-Sićevačka gorge) by high sesquiterpene alcohol shyobunol content (14.6-55.2%), the third (AL-Deja, SR-Jelašnica)
by high α-bisabolol content (31.2%-43.7%) and shyobunol
(3.2%) in population AL-Deja, the fourth (SR-Rtanj) by high
cis-sesquisabinene hydrate content (42.4%) and the fifth (GR-Olimp, GR-Ossa, SR-Trešnjica canyon) by high germecrene
D-4-ol (5.96%-61.9%), and β-eudesmol (14.9%) and epi-α-cadinol (10.2%) content in GR-Olimp population. Such a high
variability needs more research to define relationships among
populations.",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts",
title = "Variability of essential oil of different populations of Teucrium montanum L. (Lamiaceae) from Balkan peninsula",
volume = "42",
number = "1 supplement",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5257"
}

Marčetić, M., Zbiljić, M., Lakušić, D.,& Lakušić, B.. (2018). Variability of essential oil of different populations of Teucrium montanum L. (Lamiaceae) from Balkan peninsula. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 133-133.
https://hdl.handle.net/21.15107/rcub_farfar_5257

Marčetić M, Zbiljić M, Lakušić D, Lakušić B. Variability of essential oil of different populations of Teucrium montanum L. (Lamiaceae) from Balkan peninsula. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):133-133.
https://hdl.handle.net/21.15107/rcub_farfar_5257 .

Marčetić, Mirjana, Zbiljić, Miloš, Lakušić, Dmitar, Lakušić, Branislava, "Variability of essential oil of different populations of Teucrium montanum L. (Lamiaceae) from Balkan peninsula" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):133-133,
https://hdl.handle.net/21.15107/rcub_farfar_5257 .

TY  - JOUR
AU  - Slavkovska, Violeta
AU  - Lakušić, Branislava
AU  - Lakušić, Dmitar
AU  - Jančić, Radiša
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3007
AB  - The anatomical structure of the leaf and stem of nine Micromeria species from the Balkan Peninsula was investigated with the aim to establish the adaptive characteristics and traits that could be useful in the identification of species and subgeneric classification. The species included in the anatomical analysis were: Micromeria albanica (Griseb. ex K. Maly) Silic, M. thymifolia (Scop.) Fritch, M. pulegium (Rochel) Bentham, M. dalmatica Bentham of section Pseudomelissa and M. cristata (Hampe) Griseb., M. kosaninii Silic, M. juliana (L.) Bentham ex Reichenb., M. croatica (Pers.) Schott M. parviflora (Vis.) Reichenb. of section Micromeria. Variability of 17 quantitative characters of the leaf (thickness, height of adaxial and abaxial epidermis, thickness of the palisade and spongy tissue) and stem (radius, cortex thickness, thickness of the vascular cylinder, phloem, xylem, pith radius) was examined. Multivariate analysis of variance (ANOVA), principal component analysis (PCA), canonical discriminant analysis (CDA) and UPGMA clustering method based on Mahalanobis' distances were used to determine the variability structure and level of importance of the anatomical differentiation of the investigated taxa. Qualitative characters were also included in the consideration of the anatomical variability, such as leaf shape, leaf margin, position of sclerenchyma fibers in the leaf and stem. The results showed that investigated Micromeria species posses a xeromorphic general structure plan, but each species has achieved distinctive adaptations according to its specific genetic potential. The results have shown a clear difference between the species belonging to the different sections of genus Micromeria, so they have a potential diagnostic and systematic value.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Biologia
T1  - Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula
VL  - 72
IS  - 3
SP  - 277
EP  - 291
DO  - 10.1515/biolog-2017-0029
ER  - 

@article{
author = "Slavkovska, Violeta and Lakušić, Branislava and Lakušić, Dmitar and Jančić, Radiša",
year = "2017",
abstract = "The anatomical structure of the leaf and stem of nine Micromeria species from the Balkan Peninsula was investigated with the aim to establish the adaptive characteristics and traits that could be useful in the identification of species and subgeneric classification. The species included in the anatomical analysis were: Micromeria albanica (Griseb. ex K. Maly) Silic, M. thymifolia (Scop.) Fritch, M. pulegium (Rochel) Bentham, M. dalmatica Bentham of section Pseudomelissa and M. cristata (Hampe) Griseb., M. kosaninii Silic, M. juliana (L.) Bentham ex Reichenb., M. croatica (Pers.) Schott M. parviflora (Vis.) Reichenb. of section Micromeria. Variability of 17 quantitative characters of the leaf (thickness, height of adaxial and abaxial epidermis, thickness of the palisade and spongy tissue) and stem (radius, cortex thickness, thickness of the vascular cylinder, phloem, xylem, pith radius) was examined. Multivariate analysis of variance (ANOVA), principal component analysis (PCA), canonical discriminant analysis (CDA) and UPGMA clustering method based on Mahalanobis' distances were used to determine the variability structure and level of importance of the anatomical differentiation of the investigated taxa. Qualitative characters were also included in the consideration of the anatomical variability, such as leaf shape, leaf margin, position of sclerenchyma fibers in the leaf and stem. The results showed that investigated Micromeria species posses a xeromorphic general structure plan, but each species has achieved distinctive adaptations according to its specific genetic potential. The results have shown a clear difference between the species belonging to the different sections of genus Micromeria, so they have a potential diagnostic and systematic value.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Biologia",
title = "Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula",
volume = "72",
number = "3",
pages = "277-291",
doi = "10.1515/biolog-2017-0029"
}

Slavkovska, V., Lakušić, B., Lakušić, D.,& Jančić, R.. (2017). Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula. in Biologia
Walter de Gruyter Gmbh, Berlin., 72(3), 277-291.
https://doi.org/10.1515/biolog-2017-0029

Slavkovska V, Lakušić B, Lakušić D, Jančić R. Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula. in Biologia. 2017;72(3):277-291.
doi:10.1515/biolog-2017-0029 .

Slavkovska, Violeta, Lakušić, Branislava, Lakušić, Dmitar, Jančić, Radiša, "Leaf and stem anatomy of Micromeria species from the Central part of the Balkan Peninsula" in Biologia, 72, no. 3 (2017):277-291,
https://doi.org/10.1515/biolog-2017-0029 . .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Kovačević, Nada
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2953
AB  - Plant specialised metabolites like essential oils are highly variable depending on genetic and various ecological factors. The aim of the present work was to characterise essential oils of the species Seseli rigidum Waldst. & Kit. (Apiaceae) in various organs on the individual and populational levels. Geographical variability and the impact of climate and soil type on essential oil composition were also investigated. Individually sampled essential oils of roots, aerial parts and fruits of plants from seven populations were analysed by GC-FID and GC-MS. The investigated populations showed high inter-populational and especially intrapopulational variability of essential oil composition. In regard to the variability of essential oils, different chemotypes were defined. The essential oils of S. rigidum roots represented a falcarinol chemotype, oils of aerial parts constituted an alpha-pinene or alpha-pineneisabinene chemotype and fruit essential oils can be characterised as belonging to a complex sabinene/alpha-pinene/beta-phellandrene/falcarinoligermacrene B chemotype. At the species level, analysis of variance (ANOVA), principal component analysis (PCA) and canonical discriminant analysis (CDA) showed that the plant part exerted the strongest influence on the composition of essential oils. Climate had a high impact on composition of the essential oils of roots, aerial parts and fruits, while influence of the substrate was less pronounced. The variations in main compounds of essential oils based on climate or substrate were complex and specific to the plant part.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Phytochemistry
T1  - Habitat-related variation in composition of the essential oil of Seseli rigidum Waldst. & Kit. (Apiaceae)
VL  - 135
SP  - 80
EP  - 92
DO  - 10.1016/j.phytochem.2016.12.004
ER  - 

@article{
author = "Marčetić, Mirjana and Kovačević, Nada and Lakušić, Dmitar and Lakušić, Branislava",
year = "2017",
abstract = "Plant specialised metabolites like essential oils are highly variable depending on genetic and various ecological factors. The aim of the present work was to characterise essential oils of the species Seseli rigidum Waldst. & Kit. (Apiaceae) in various organs on the individual and populational levels. Geographical variability and the impact of climate and soil type on essential oil composition were also investigated. Individually sampled essential oils of roots, aerial parts and fruits of plants from seven populations were analysed by GC-FID and GC-MS. The investigated populations showed high inter-populational and especially intrapopulational variability of essential oil composition. In regard to the variability of essential oils, different chemotypes were defined. The essential oils of S. rigidum roots represented a falcarinol chemotype, oils of aerial parts constituted an alpha-pinene or alpha-pineneisabinene chemotype and fruit essential oils can be characterised as belonging to a complex sabinene/alpha-pinene/beta-phellandrene/falcarinoligermacrene B chemotype. At the species level, analysis of variance (ANOVA), principal component analysis (PCA) and canonical discriminant analysis (CDA) showed that the plant part exerted the strongest influence on the composition of essential oils. Climate had a high impact on composition of the essential oils of roots, aerial parts and fruits, while influence of the substrate was less pronounced. The variations in main compounds of essential oils based on climate or substrate were complex and specific to the plant part.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Phytochemistry",
title = "Habitat-related variation in composition of the essential oil of Seseli rigidum Waldst. & Kit. (Apiaceae)",
volume = "135",
pages = "80-92",
doi = "10.1016/j.phytochem.2016.12.004"
}

Marčetić, M., Kovačević, N., Lakušić, D.,& Lakušić, B.. (2017). Habitat-related variation in composition of the essential oil of Seseli rigidum Waldst. & Kit. (Apiaceae). in Phytochemistry
Pergamon-Elsevier Science Ltd, Oxford., 135, 80-92.
https://doi.org/10.1016/j.phytochem.2016.12.004

Marčetić M, Kovačević N, Lakušić D, Lakušić B. Habitat-related variation in composition of the essential oil of Seseli rigidum Waldst. & Kit. (Apiaceae). in Phytochemistry. 2017;135:80-92.
doi:10.1016/j.phytochem.2016.12.004 .

Marčetić, Mirjana, Kovačević, Nada, Lakušić, Dmitar, Lakušić, Branislava, "Habitat-related variation in composition of the essential oil of Seseli rigidum Waldst. & Kit. (Apiaceae)" in Phytochemistry, 135 (2017):80-92,
https://doi.org/10.1016/j.phytochem.2016.12.004 . .

TY  - JOUR
AU  - Zlatković, Bojan
AU  - Mitić, Zorica S.
AU  - Jovanović, S.
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
AU  - Rajković, J.
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2850
AB  - Sedum album is a succulent plant, with phenotypic variability resulting in delimitation of a number of taxa with diverse taxonomic recognition. We analyzed the taxa from Sedum album in broad sense (S. album, S. micranthum, S. athoum and S. serpentini) from Balkan Peninsula, to check whether the variability of their epidermal structures and epicuticular wax composition corresponds to their taxonomic reliability. Epidermal structures and epicuticular waxes were analyzed using light and scanning electron microscopy, gas chromatography-mass spectrometry and gas chromatography-flame ionization detector chromatography and multivariate statistical techniques. Analyses of studied taxa have shown that significant systematic characters include adaxial epidermal and guard cell length, stomatal frequency and subsidiary cells area on abaxial epidermis. The content of n-alkanes C-29, C-30, C-27, C-32 and C-33 was shown to be valuable criterion for their phytochemical delimitation. The comparison of epicuticular wax microstructures has shown no significant differences. Results indicate presence of two (micromorphology) or three (phytochemistry) well-defined groups of populations, with S. serpentini manifesting a considerable level of separation toward the others. Sedum athoum have shown weak to moderate degree of differentiation, while S. micranthum has shown general resemblance to S. album.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Plant Biosystems
T1  - Epidermal structures and composition of epicuticular waxes of Sedum album sensu lato (Crassulaceae) in Balkan Peninsula
VL  - 151
IS  - 6
SP  - 974
EP  - 984
DO  - 10.1080/11263504.2016.1218971
ER  - 

@article{
author = "Zlatković, Bojan and Mitić, Zorica S. and Jovanović, S. and Lakušić, Dmitar and Lakušić, Branislava and Rajković, J. and Stojanović, Gordana",
year = "2017",
abstract = "Sedum album is a succulent plant, with phenotypic variability resulting in delimitation of a number of taxa with diverse taxonomic recognition. We analyzed the taxa from Sedum album in broad sense (S. album, S. micranthum, S. athoum and S. serpentini) from Balkan Peninsula, to check whether the variability of their epidermal structures and epicuticular wax composition corresponds to their taxonomic reliability. Epidermal structures and epicuticular waxes were analyzed using light and scanning electron microscopy, gas chromatography-mass spectrometry and gas chromatography-flame ionization detector chromatography and multivariate statistical techniques. Analyses of studied taxa have shown that significant systematic characters include adaxial epidermal and guard cell length, stomatal frequency and subsidiary cells area on abaxial epidermis. The content of n-alkanes C-29, C-30, C-27, C-32 and C-33 was shown to be valuable criterion for their phytochemical delimitation. The comparison of epicuticular wax microstructures has shown no significant differences. Results indicate presence of two (micromorphology) or three (phytochemistry) well-defined groups of populations, with S. serpentini manifesting a considerable level of separation toward the others. Sedum athoum have shown weak to moderate degree of differentiation, while S. micranthum has shown general resemblance to S. album.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Plant Biosystems",
title = "Epidermal structures and composition of epicuticular waxes of Sedum album sensu lato (Crassulaceae) in Balkan Peninsula",
volume = "151",
number = "6",
pages = "974-984",
doi = "10.1080/11263504.2016.1218971"
}

Zlatković, B., Mitić, Z. S., Jovanović, S., Lakušić, D., Lakušić, B., Rajković, J.,& Stojanović, G.. (2017). Epidermal structures and composition of epicuticular waxes of Sedum album sensu lato (Crassulaceae) in Balkan Peninsula. in Plant Biosystems
Taylor & Francis Ltd, Abingdon., 151(6), 974-984.
https://doi.org/10.1080/11263504.2016.1218971

Zlatković B, Mitić ZS, Jovanović S, Lakušić D, Lakušić B, Rajković J, Stojanović G. Epidermal structures and composition of epicuticular waxes of Sedum album sensu lato (Crassulaceae) in Balkan Peninsula. in Plant Biosystems. 2017;151(6):974-984.
doi:10.1080/11263504.2016.1218971 .

Zlatković, Bojan, Mitić, Zorica S., Jovanović, S., Lakušić, Dmitar, Lakušić, Branislava, Rajković, J., Stojanović, Gordana, "Epidermal structures and composition of epicuticular waxes of Sedum album sensu lato (Crassulaceae) in Balkan Peninsula" in Plant Biosystems, 151, no. 6 (2017):974-984,
https://doi.org/10.1080/11263504.2016.1218971 . .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Suručić, Relja V.
AU  - Kovačević, Nada
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2788
AB  - The chemical composition of the essential oils of Seseli gracile Waldst. & Kit. from natural habitat (Derdap Gorge, Serbia) and from cultivated plants (Belgrade, Serbia) were characterized. The essential oils of the root, aerial parts, inflorescence and fruit were analyzed by GC/MS and GC/FID. Monoterpene hydrocarbons were the main compounds in the essential oil of aerial parts (45.2-93.0 %), inflorescences (84.1 and 90.0 %) and fruit (85.0 %). Polyacetylenes (38.8 and 87.6 %) were dominant in the essential oil of root. The cluster analysis revealed that there were significant differences in the chemical composition of the S. gracile oils at different phenological stages. On the other hand, essential oils from the aerial parts from natural and cultivated plants showed quite uniform qualitative composition. The aerial parts essential oil from natural habitat contained higher content of para-cymene (mean values 17.3 vs. 6.5 %) and lower amounts of terpinolene (mean values 23.1 vs. 49.9 %). Also polyacetylene falcarinol was present only in the aerial parts samples from natural habitat. The essential oil of inflorescences from natural habitat contained higher concentration of terpinolene, quite similar amount of para-cymene and lower content of a-pinene.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions
VL  - 82
IS  - 7-8
SP  - 815
EP  - 824
DO  - 10.2298/JSC170320059M
ER  - 

@article{
author = "Marčetić, Mirjana and Suručić, Relja V. and Kovačević, Nada and Lakušić, Dmitar and Lakušić, Branislava",
year = "2017",
abstract = "The chemical composition of the essential oils of Seseli gracile Waldst. & Kit. from natural habitat (Derdap Gorge, Serbia) and from cultivated plants (Belgrade, Serbia) were characterized. The essential oils of the root, aerial parts, inflorescence and fruit were analyzed by GC/MS and GC/FID. Monoterpene hydrocarbons were the main compounds in the essential oil of aerial parts (45.2-93.0 %), inflorescences (84.1 and 90.0 %) and fruit (85.0 %). Polyacetylenes (38.8 and 87.6 %) were dominant in the essential oil of root. The cluster analysis revealed that there were significant differences in the chemical composition of the S. gracile oils at different phenological stages. On the other hand, essential oils from the aerial parts from natural and cultivated plants showed quite uniform qualitative composition. The aerial parts essential oil from natural habitat contained higher content of para-cymene (mean values 17.3 vs. 6.5 %) and lower amounts of terpinolene (mean values 23.1 vs. 49.9 %). Also polyacetylene falcarinol was present only in the aerial parts samples from natural habitat. The essential oil of inflorescences from natural habitat contained higher concentration of terpinolene, quite similar amount of para-cymene and lower content of a-pinene.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions",
volume = "82",
number = "7-8",
pages = "815-824",
doi = "10.2298/JSC170320059M"
}

Marčetić, M., Suručić, R. V., Kovačević, N., Lakušić, D.,& Lakušić, B.. (2017). Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 82(7-8), 815-824.
https://doi.org/10.2298/JSC170320059M

Marčetić M, Suručić RV, Kovačević N, Lakušić D, Lakušić B. Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions. in Journal of the Serbian Chemical Society. 2017;82(7-8):815-824.
doi:10.2298/JSC170320059M .

Marčetić, Mirjana, Suručić, Relja V., Kovačević, Nada, Lakušić, Dmitar, Lakušić, Branislava, "Essential oil composition of different parts of endemic species Seseli gracile Waldst. & Kit. (Apiaceae) from natural and cultivated conditions" in Journal of the Serbian Chemical Society, 82, no. 7-8 (2017):815-824,
https://doi.org/10.2298/JSC170320059M . .

TY  - JOUR
AU  - Kovačević, Nada
AU  - Marčetić, Mirjana
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2612
AB  - The chemical composition of the essential oils of different parts of Seseli annuum from Serbia was investigated. beta-Selinene (0-21.4 %), germacrene D (3.4-19.1 %), caryophyllene oxide (1.2-18.1 %), germacrene A (0-14.6%), alpha-selinene (0-12.4 %), beta-elemene (0-11.4 %), E-caryophyllene (3.2-10.3 %), alpha-pinene (0.6-7.5 %), vetiselinenol (0-6.3 %) and isodaucene (0-5.4 %) were dominant compounds in the analysed essential oils. The cluster analysis revealed separation of two groups of essential oils: the first group of oils from fruit, inflorescence and aerial parts in flowering phase with high beta-selinene and alpha-selinene content and the second group of oils from leaf, stem and rosette with higher caryophyllene oxide amounts. The essential oil of rosette was separated from the oils of leaf or stem and contained the highest caryophyllene oxide content while alpha-selinene and beta-selinene were completely absent.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Essential Oil Bearing Plants
T1  - Composition of the Essential Oils of Different Parts of Seseli annuum L. (Apiaceae)
VL  - 19
IS  - 3
SP  - 671
EP  - 677
DO  - 10.1080/0972060X.2014.901604
ER  - 

@article{
author = "Kovačević, Nada and Marčetić, Mirjana and Lakušić, Dmitar and Lakušić, Branislava",
year = "2016",
abstract = "The chemical composition of the essential oils of different parts of Seseli annuum from Serbia was investigated. beta-Selinene (0-21.4 %), germacrene D (3.4-19.1 %), caryophyllene oxide (1.2-18.1 %), germacrene A (0-14.6%), alpha-selinene (0-12.4 %), beta-elemene (0-11.4 %), E-caryophyllene (3.2-10.3 %), alpha-pinene (0.6-7.5 %), vetiselinenol (0-6.3 %) and isodaucene (0-5.4 %) were dominant compounds in the analysed essential oils. The cluster analysis revealed separation of two groups of essential oils: the first group of oils from fruit, inflorescence and aerial parts in flowering phase with high beta-selinene and alpha-selinene content and the second group of oils from leaf, stem and rosette with higher caryophyllene oxide amounts. The essential oil of rosette was separated from the oils of leaf or stem and contained the highest caryophyllene oxide content while alpha-selinene and beta-selinene were completely absent.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Essential Oil Bearing Plants",
title = "Composition of the Essential Oils of Different Parts of Seseli annuum L. (Apiaceae)",
volume = "19",
number = "3",
pages = "671-677",
doi = "10.1080/0972060X.2014.901604"
}

Kovačević, N., Marčetić, M., Lakušić, D.,& Lakušić, B.. (2016). Composition of the Essential Oils of Different Parts of Seseli annuum L. (Apiaceae). in Journal of Essential Oil Bearing Plants
Taylor & Francis Ltd, Abingdon., 19(3), 671-677.
https://doi.org/10.1080/0972060X.2014.901604

Kovačević N, Marčetić M, Lakušić D, Lakušić B. Composition of the Essential Oils of Different Parts of Seseli annuum L. (Apiaceae). in Journal of Essential Oil Bearing Plants. 2016;19(3):671-677.
doi:10.1080/0972060X.2014.901604 .

Kovačević, Nada, Marčetić, Mirjana, Lakušić, Dmitar, Lakušić, Branislava, "Composition of the Essential Oils of Different Parts of Seseli annuum L. (Apiaceae)" in Journal of Essential Oil Bearing Plants, 19, no. 3 (2016):671-677,
https://doi.org/10.1080/0972060X.2014.901604 . .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Milenković, Marina
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2533
AB  - Hypericum aegypticum subsp. webbii is an evergreen shrub spread in Mediterranean part of central and southeastern Europe. The chemical composition and antimicrobial activity of the essential oil and MeOH extract of H. aegypticum subsp. webbii were investigated. The monoterpenes alpha-pinene (63.4 - 68.5%) and beta-pinene (16.9 - 17.0%) were main compounds in the volatile oil from aerial parts. In the cluster analysis, the essential oil of H. aegypticum subsp. webbii was separated and chemically different from the oil of other subspecies of H. aegypticum as well as other Hypericum species from Greece. SIMPER analysis revealed that alpha-pinene (24.79%) was the component that contributed the most to differences between all oils. Also, there was extremely high overall dissimilarity between three subspecies of H. aegypticum. MeOH extract of aerial parts of H. aegypticum subsp. webbii contained flavonoids rutin (56.4 +/- 0.9 mg/g), hyperoside and quercetin, and phenolic acids chlorogenic and caffeic acid, while naphthodianthrones were not detected. The antimicrobial activity of essential oil was moderate (MIC from 100 to >200 mu g/ml), while MeOH extract inhibited the growth of Gram-positive bacteria Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, and Micrococcus luteus (MIC 50 - 100 mu g/ml), more pronounced than the extract of H. perforatum (MIC 200 - >200 mu g/ml).
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Chemistry & Biodiversity
T1  - Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson
VL  - 13
IS  - 4
SP  - 427
EP  - 436
DO  - 10.1002/cbdv.201500119
ER  - 

@article{
author = "Marčetić, Mirjana and Milenković, Marina and Lakušić, Dmitar and Lakušić, Branislava",
year = "2016",
abstract = "Hypericum aegypticum subsp. webbii is an evergreen shrub spread in Mediterranean part of central and southeastern Europe. The chemical composition and antimicrobial activity of the essential oil and MeOH extract of H. aegypticum subsp. webbii were investigated. The monoterpenes alpha-pinene (63.4 - 68.5%) and beta-pinene (16.9 - 17.0%) were main compounds in the volatile oil from aerial parts. In the cluster analysis, the essential oil of H. aegypticum subsp. webbii was separated and chemically different from the oil of other subspecies of H. aegypticum as well as other Hypericum species from Greece. SIMPER analysis revealed that alpha-pinene (24.79%) was the component that contributed the most to differences between all oils. Also, there was extremely high overall dissimilarity between three subspecies of H. aegypticum. MeOH extract of aerial parts of H. aegypticum subsp. webbii contained flavonoids rutin (56.4 +/- 0.9 mg/g), hyperoside and quercetin, and phenolic acids chlorogenic and caffeic acid, while naphthodianthrones were not detected. The antimicrobial activity of essential oil was moderate (MIC from 100 to >200 mu g/ml), while MeOH extract inhibited the growth of Gram-positive bacteria Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, and Micrococcus luteus (MIC 50 - 100 mu g/ml), more pronounced than the extract of H. perforatum (MIC 200 - >200 mu g/ml).",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson",
volume = "13",
number = "4",
pages = "427-436",
doi = "10.1002/cbdv.201500119"
}

Marčetić, M., Milenković, M., Lakušić, D.,& Lakušić, B.. (2016). Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson. in Chemistry & Biodiversity
Wiley-VCH Verlag GMBH, Weinheim., 13(4), 427-436.
https://doi.org/10.1002/cbdv.201500119

Marčetić M, Milenković M, Lakušić D, Lakušić B. Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson. in Chemistry & Biodiversity. 2016;13(4):427-436.
doi:10.1002/cbdv.201500119 .

Marčetić, Mirjana, Milenković, Marina, Lakušić, Dmitar, Lakušić, Branislava, "Chemical Composition and Antimicrobial Activity of the Essential Oil and Methanol Extract of Hypericum aegypticum subsp. webbii (Spach) N. Robson" in Chemistry & Biodiversity, 13, no. 4 (2016):427-436,
https://doi.org/10.1002/cbdv.201500119 . .

TY  - CONF
AU  - Milenković, Marina
AU  - Ristić, Mihailo
AU  - Lakušić, Dmitar
AU  - Slavkovska, Violeta
AU  - Lakušić, Branislava
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5528
AB  - In this study the chemical composition and antimicrobial activity of the essential oils of Satureja horvatii cultivated plants (Belgrade, Serbia) were characterized. S. horvatii is an endemo-relict species of Montenegro. The essential oils were isolated, from the aerial parts of the plants at different phenological stages, by hydrodistillation and analyzed by GC and GC/MS. Chemical compositions of the S. horvatii oils showed differences between the plants at different phenological stages. The main compounds of the essential oil from plants at the flowering stage were linalool (53.5%), thymol (17.9%), and α-terpineol (8.5%). At the stage of full fruiting the percentage of linalool (58.4–59.5%) and α-terpineol (11.8-12.3%) increased, while those of thymol (4.6–7.6%) significantly decreased. The antimicrobial activity of tested oils was evaluated against ten standard strains of microorganisms.The working solutions of essential oils (40 μL/mL) were prepared by dissolving the essential oils (EOs) in DMSO and subsequent mixing with Müller–Hinton or Sabouraud dextrose broth. The EOs were tested in the final concetration range of 0.313 - 20.000 μL/mL. All analyzed oils showed the greatest and uniform antimicrobial activity against the yeast (Candida albicans). The essential oil isolated from plant in the flowering stage showed the maximum activity against Staphylococcus epidermidis and Staphylococcus aureus. This oil exhibited moderate activity against the Enterococcus faecalis and weak activity against the other microorganisms. Oils isolated from plants at fruiting stage showed moderate activity against Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa and weak activity against the other microorganisms.
PB  - Marmara University Faculty  of Pharmacy
C3  - Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book
T1  - Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae)
SP  - 70
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5528
ER  - 

@conference{
author = "Milenković, Marina and Ristić, Mihailo and Lakušić, Dmitar and Slavkovska, Violeta and Lakušić, Branislava",
year = "2014",
abstract = "In this study the chemical composition and antimicrobial activity of the essential oils of Satureja horvatii cultivated plants (Belgrade, Serbia) were characterized. S. horvatii is an endemo-relict species of Montenegro. The essential oils were isolated, from the aerial parts of the plants at different phenological stages, by hydrodistillation and analyzed by GC and GC/MS. Chemical compositions of the S. horvatii oils showed differences between the plants at different phenological stages. The main compounds of the essential oil from plants at the flowering stage were linalool (53.5%), thymol (17.9%), and α-terpineol (8.5%). At the stage of full fruiting the percentage of linalool (58.4–59.5%) and α-terpineol (11.8-12.3%) increased, while those of thymol (4.6–7.6%) significantly decreased. The antimicrobial activity of tested oils was evaluated against ten standard strains of microorganisms.The working solutions of essential oils (40 μL/mL) were prepared by dissolving the essential oils (EOs) in DMSO and subsequent mixing with Müller–Hinton or Sabouraud dextrose broth. The EOs were tested in the final concetration range of 0.313 - 20.000 μL/mL. All analyzed oils showed the greatest and uniform antimicrobial activity against the yeast (Candida albicans). The essential oil isolated from plant in the flowering stage showed the maximum activity against Staphylococcus epidermidis and Staphylococcus aureus. This oil exhibited moderate activity against the Enterococcus faecalis and weak activity against the other microorganisms. Oils isolated from plants at fruiting stage showed moderate activity against Staphylococcus epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa and weak activity against the other microorganisms.",
publisher = "Marmara University Faculty  of Pharmacy",
journal = "Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book",
title = "Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae)",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5528"
}

Milenković, M., Ristić, M., Lakušić, D., Slavkovska, V.,& Lakušić, B.. (2014). Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae). in Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book
Marmara University Faculty  of Pharmacy., 70-70.
https://hdl.handle.net/21.15107/rcub_farfar_5528

Milenković M, Ristić M, Lakušić D, Slavkovska V, Lakušić B. Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae). in Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book. 2014;:70-70.
https://hdl.handle.net/21.15107/rcub_farfar_5528 .

Milenković, Marina, Ristić, Mihailo, Lakušić, Dmitar, Slavkovska, Violeta, Lakušić, Branislava, "Chemical composition and antimicrobial activity Chemical Composition and Antimicrobial Activity of the Essential Oil from Cultivated Satureja horvatii Silić (Lamiaceae)" in Fourth International Meeting on Pharmacy & Pharmaceutical Sciences, Abstract book (2014):70-70,
https://hdl.handle.net/21.15107/rcub_farfar_5528 .

TY  - JOUR
AU  - Lakušić, Branislava
AU  - Lakušić, Dmitar
AU  - Ristić, Mihailo
AU  - Marčetić, Mirjana
AU  - Slavkovska, Violeta
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2208
AB  - Seasonal variations in the composition of the essential oils obtained from the same individual (of the same genotype) of Lavandula angustifolia cultivated in Belgrade were determined by GC and GC/MS. The main constituents were 1,8-cineole (7.1-48.4%), linalool (0.1-38.7%), borneol (10.9-27.7%), beta-phellandrene (0.5-21.2%) and camphor (1.5-15.8%). Cluster analysis showed that the 21 samples collected each month during the vegetation cycle were separable into three main clades with different compositions of essential oils. In the shoots with flowers, inflorescences and fruits of clade I, linalool is dominant, in the young leaves before flowering and old leaves of clade II, 1,8-cineole is dominant. In the young and incompletely developed leaves of clade III,beta-phellandrene is dominant. The composition of the essential oils of lavender depended on the plant part and the stage of development.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Natural Product Communications
T1  - Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae)
VL  - 9
IS  - 6
SP  - 859
EP  - 862
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2208
ER  - 

@article{
author = "Lakušić, Branislava and Lakušić, Dmitar and Ristić, Mihailo and Marčetić, Mirjana and Slavkovska, Violeta",
year = "2014",
abstract = "Seasonal variations in the composition of the essential oils obtained from the same individual (of the same genotype) of Lavandula angustifolia cultivated in Belgrade were determined by GC and GC/MS. The main constituents were 1,8-cineole (7.1-48.4%), linalool (0.1-38.7%), borneol (10.9-27.7%), beta-phellandrene (0.5-21.2%) and camphor (1.5-15.8%). Cluster analysis showed that the 21 samples collected each month during the vegetation cycle were separable into three main clades with different compositions of essential oils. In the shoots with flowers, inflorescences and fruits of clade I, linalool is dominant, in the young leaves before flowering and old leaves of clade II, 1,8-cineole is dominant. In the young and incompletely developed leaves of clade III,beta-phellandrene is dominant. The composition of the essential oils of lavender depended on the plant part and the stage of development.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Natural Product Communications",
title = "Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae)",
volume = "9",
number = "6",
pages = "859-862",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2208"
}

Lakušić, B., Lakušić, D., Ristić, M., Marčetić, M.,& Slavkovska, V.. (2014). Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae). in Natural Product Communications
Sage Publications Inc, Thousand Oaks., 9(6), 859-862.
https://hdl.handle.net/21.15107/rcub_farfar_2208

Lakušić B, Lakušić D, Ristić M, Marčetić M, Slavkovska V. Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae). in Natural Product Communications. 2014;9(6):859-862.
https://hdl.handle.net/21.15107/rcub_farfar_2208 .

Lakušić, Branislava, Lakušić, Dmitar, Ristić, Mihailo, Marčetić, Mirjana, Slavkovska, Violeta, "Seasonal Variations in the Composition of the Essential Oils of Lavandula angustifolia (Lamiacae)" in Natural Product Communications, 9, no. 6 (2014):859-862,
https://hdl.handle.net/21.15107/rcub_farfar_2208 .

TY  - JOUR
AU  - Lakušić, Dmitar
AU  - Lakušić, Branislava
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2120
AB  - Inter-population differentiation of the species Teucrium montanum was analyzed on the basis of morpho-anatomical variability of its thirteen populations distributed between 60 and 1750 m above sea level in the Mediterranean and Continental region of the central Balkan Peninsula. Descriptive statistics were performed for each of 28 continuous quantitative characters related to leaf and stem morphology and anatomy. The hypothesis of morphological separation of populations was tested using canonical discriminant analysis (CDA), and discriminant function analysis was used to estimate the contribution of individual characters to overall discrimination. The discrimination between groups in CDA was predominantly based on characteristics of the external structure on the leaf surface, while the internal structure of the leaf and stem did not show such significant differences between the 13 populations. The morpho-anatomical analysis of plants from these populations has confirmed that the species T. montanum belongs to evergreen microphyllic xeromorphic semi-bushes. Statistical analysis revealed four group of populations with significant morphological differentiation. One part of the observed anatomical variability could be explained as an adaptive response to different geographical and recent environmental factors, and another part seemed to be caused by genetic or evolutionary factors.
AB  - Interpopulaciona diferencijacija vrste Teucrium montanum je analizirana na osnovu varijabilnosti morfo- anatomskih karakteristika lista i stabla u okviru 13 populacija koje su rasprostranjene između 60 i 1750 m nadmorske visine, u mediteranskom i kontinentalnom delu Centralnog Balkana (Crna Gora i Srbija). Statističkom analizom je obuhvaćeno 28 kvantitativnih karaktera anatomije i morfologije lista i stabla. Hipoteza o morfo-anatomskoj diferencijaciji populacija testirana je kanonskom diskriminantnom analizom (CDA), dok je diskriminantnom funkcijskom analizom procenjen značaj pojedinačnih karaktera u diferencijaciji populacija. Razdvajanje grupa je pretežno zasnovano na karakterima površinske strukture listova, dok unutrašnja građa lista i stabla nije pokazala tako značajne uticaj na ustanovljene razlike između analiziranih populacija. Na osnovu morfo-anatomskih analiza, utvrđeno je da vrsta T. montanum pripada grupi zimzelenih, mikrofilnih kseromorfnih polužbunova. Statistička analiza je pokazala postojanje četiri grupe populacija čija se varijabilnost, s jedne strane, može tumačiti kao adaptivni odgovor na različite ekološke uslove staništa kao i geografski položaj istraživanih populacija. S druge strane, deo ustanovljene varijabilnosti se izgleda može objasniti i odredjenim genetičkim i evolutivnim faktorima.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Morpho-anatomical differentiation of the species Teucrium montanum (Lamiaceae) in the central Balkan Peninsula
T1  - Morfo-anatomska diferencijacija vrste teucrium montanum (Lamiaceae) na prostoru Centralnog Balkana
VL  - 38
IS  - 1
SP  - 109
EP  - 120
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2120
ER  - 

@article{
author = "Lakušić, Dmitar and Lakušić, Branislava",
year = "2014",
abstract = "Inter-population differentiation of the species Teucrium montanum was analyzed on the basis of morpho-anatomical variability of its thirteen populations distributed between 60 and 1750 m above sea level in the Mediterranean and Continental region of the central Balkan Peninsula. Descriptive statistics were performed for each of 28 continuous quantitative characters related to leaf and stem morphology and anatomy. The hypothesis of morphological separation of populations was tested using canonical discriminant analysis (CDA), and discriminant function analysis was used to estimate the contribution of individual characters to overall discrimination. The discrimination between groups in CDA was predominantly based on characteristics of the external structure on the leaf surface, while the internal structure of the leaf and stem did not show such significant differences between the 13 populations. The morpho-anatomical analysis of plants from these populations has confirmed that the species T. montanum belongs to evergreen microphyllic xeromorphic semi-bushes. Statistical analysis revealed four group of populations with significant morphological differentiation. One part of the observed anatomical variability could be explained as an adaptive response to different geographical and recent environmental factors, and another part seemed to be caused by genetic or evolutionary factors., Interpopulaciona diferencijacija vrste Teucrium montanum je analizirana na osnovu varijabilnosti morfo- anatomskih karakteristika lista i stabla u okviru 13 populacija koje su rasprostranjene između 60 i 1750 m nadmorske visine, u mediteranskom i kontinentalnom delu Centralnog Balkana (Crna Gora i Srbija). Statističkom analizom je obuhvaćeno 28 kvantitativnih karaktera anatomije i morfologije lista i stabla. Hipoteza o morfo-anatomskoj diferencijaciji populacija testirana je kanonskom diskriminantnom analizom (CDA), dok je diskriminantnom funkcijskom analizom procenjen značaj pojedinačnih karaktera u diferencijaciji populacija. Razdvajanje grupa je pretežno zasnovano na karakterima površinske strukture listova, dok unutrašnja građa lista i stabla nije pokazala tako značajne uticaj na ustanovljene razlike između analiziranih populacija. Na osnovu morfo-anatomskih analiza, utvrđeno je da vrsta T. montanum pripada grupi zimzelenih, mikrofilnih kseromorfnih polužbunova. Statistička analiza je pokazala postojanje četiri grupe populacija čija se varijabilnost, s jedne strane, može tumačiti kao adaptivni odgovor na različite ekološke uslove staništa kao i geografski položaj istraživanih populacija. S druge strane, deo ustanovljene varijabilnosti se izgleda može objasniti i odredjenim genetičkim i evolutivnim faktorima.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Morpho-anatomical differentiation of the species Teucrium montanum (Lamiaceae) in the central Balkan Peninsula, Morfo-anatomska diferencijacija vrste teucrium montanum (Lamiaceae) na prostoru Centralnog Balkana",
volume = "38",
number = "1",
pages = "109-120",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2120"
}

Lakušić, D.,& Lakušić, B.. (2014). Morpho-anatomical differentiation of the species Teucrium montanum (Lamiaceae) in the central Balkan Peninsula. in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 38(1), 109-120.
https://hdl.handle.net/21.15107/rcub_farfar_2120

Lakušić D, Lakušić B. Morpho-anatomical differentiation of the species Teucrium montanum (Lamiaceae) in the central Balkan Peninsula. in Botanica Serbica. 2014;38(1):109-120.
https://hdl.handle.net/21.15107/rcub_farfar_2120 .

Lakušić, Dmitar, Lakušić, Branislava, "Morpho-anatomical differentiation of the species Teucrium montanum (Lamiaceae) in the central Balkan Peninsula" in Botanica Serbica, 38, no. 1 (2014):109-120,
https://hdl.handle.net/21.15107/rcub_farfar_2120 .

TY  - JOUR
AU  - Janković, Ivana B.
AU  - Drobac, Milica
AU  - Lakušić, Dmitar
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2075
AB  - During the past few years, the isophylloid Campanula pyramidalis complex has been the subject of studies aimed at an improved understanding of the relationships within it. The center of distribution of the C. pyramidalis complex is in the Balkan Peninsula with some smaller parts of the area located in the south Apennines. Although 21 taxa of the C. pyramidalis complex were described, only four species are accepted: C. pyramidalis, C. versicolor, C. secundiflora and C. austroadriatica. In the present study, we propose compounds of the methanolic leaf extract as possible chemotaxonomic markers for the C. pyramidalis complex. Eleven flavonoids and two phenolic acids were detected in leaf extract using high-performance liquid chromatography with diode-array detection analysis. The investigated taxa of the C. pyramidal is complex differ in terms of the composition of the methanolic leaf extract. Clustering of investigated taxa is not completely consistent with the previously reported molecular and morphometric data
PB  - Univ Zagreb, Fac Science, Div Biology, Zagreb
T2  - Acta Botanica Croatica
T1  - Compounds of the methanolic leaf extract as chemotaxonomic markers for the Campanula pyramidalis complex (Campanulaceae)
VL  - 73
IS  - 2
SP  - 481
EP  - 490
DO  - 10.2478/botcro-2014-0013
ER  - 

@article{
author = "Janković, Ivana B. and Drobac, Milica and Lakušić, Dmitar",
year = "2014",
abstract = "During the past few years, the isophylloid Campanula pyramidalis complex has been the subject of studies aimed at an improved understanding of the relationships within it. The center of distribution of the C. pyramidalis complex is in the Balkan Peninsula with some smaller parts of the area located in the south Apennines. Although 21 taxa of the C. pyramidalis complex were described, only four species are accepted: C. pyramidalis, C. versicolor, C. secundiflora and C. austroadriatica. In the present study, we propose compounds of the methanolic leaf extract as possible chemotaxonomic markers for the C. pyramidalis complex. Eleven flavonoids and two phenolic acids were detected in leaf extract using high-performance liquid chromatography with diode-array detection analysis. The investigated taxa of the C. pyramidal is complex differ in terms of the composition of the methanolic leaf extract. Clustering of investigated taxa is not completely consistent with the previously reported molecular and morphometric data",
publisher = "Univ Zagreb, Fac Science, Div Biology, Zagreb",
journal = "Acta Botanica Croatica",
title = "Compounds of the methanolic leaf extract as chemotaxonomic markers for the Campanula pyramidalis complex (Campanulaceae)",
volume = "73",
number = "2",
pages = "481-490",
doi = "10.2478/botcro-2014-0013"
}

Janković, I. B., Drobac, M.,& Lakušić, D.. (2014). Compounds of the methanolic leaf extract as chemotaxonomic markers for the Campanula pyramidalis complex (Campanulaceae). in Acta Botanica Croatica
Univ Zagreb, Fac Science, Div Biology, Zagreb., 73(2), 481-490.
https://doi.org/10.2478/botcro-2014-0013

Janković IB, Drobac M, Lakušić D. Compounds of the methanolic leaf extract as chemotaxonomic markers for the Campanula pyramidalis complex (Campanulaceae). in Acta Botanica Croatica. 2014;73(2):481-490.
doi:10.2478/botcro-2014-0013 .

Janković, Ivana B., Drobac, Milica, Lakušić, Dmitar, "Compounds of the methanolic leaf extract as chemotaxonomic markers for the Campanula pyramidalis complex (Campanulaceae)" in Acta Botanica Croatica, 73, no. 2 (2014):481-490,
https://doi.org/10.2478/botcro-2014-0013 . .

TY  - JOUR
AU  - Lakušić, Dmitar
AU  - Ristić, Mihailo
AU  - Slavkovska, Violeta
AU  - Lakušić, Branislava
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1937
AB  - Seasonal variations in the composition of the essential oils obtained from rosemary plants of the same genotype cultivated in Belgrade were determined by GC and GC/MS. The main constituents were camphor (18.2 - 28.1%), 1,8-cineole (6.4-18.0%), alpha-pinene (9.7-13.5%), borneol (4.4-9.5%), camphene (5.1-8.7%), beta-pinene (2.1-8.1%), beta-phellandrene (4.6-6.5%), myrcene (3.4-5.9%) and bornyl acetate (0.2-7.9%). Cluster analysis showed that 16 samples that had been collected each month during the vegetative cycle can be separated into three main clades with different compositions of essential oils. In the shoots with fruits ('fruits' - Clade I) and shoots with developed leaves ('old shoots' - Clade III) camphor is dominant. In shoots with young and incompletely developed leaves ('young shoot' - Clade II) camphor and 1,8-cineole had almost the same concentration. The fact that the same genotype during the growing seasons can synthesize oils that are so different that they can be classified as different chemotypes confirms the opinion that the chemical composition of essential oils sometimes critically depends on the time of collection. Also, for the definition of chemotypes it is not enough to base this on a chemical analysis of an oil from one phenophase only.
PB  - Natural Products Inc, Westerville
T2  - Natural Product Communications
T1  - Seasonal Variations in the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis, Lamiaceae)
VL  - 8
IS  - 1
SP  - 131
EP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1937
ER  - 

@article{
author = "Lakušić, Dmitar and Ristić, Mihailo and Slavkovska, Violeta and Lakušić, Branislava",
year = "2013",
abstract = "Seasonal variations in the composition of the essential oils obtained from rosemary plants of the same genotype cultivated in Belgrade were determined by GC and GC/MS. The main constituents were camphor (18.2 - 28.1%), 1,8-cineole (6.4-18.0%), alpha-pinene (9.7-13.5%), borneol (4.4-9.5%), camphene (5.1-8.7%), beta-pinene (2.1-8.1%), beta-phellandrene (4.6-6.5%), myrcene (3.4-5.9%) and bornyl acetate (0.2-7.9%). Cluster analysis showed that 16 samples that had been collected each month during the vegetative cycle can be separated into three main clades with different compositions of essential oils. In the shoots with fruits ('fruits' - Clade I) and shoots with developed leaves ('old shoots' - Clade III) camphor is dominant. In shoots with young and incompletely developed leaves ('young shoot' - Clade II) camphor and 1,8-cineole had almost the same concentration. The fact that the same genotype during the growing seasons can synthesize oils that are so different that they can be classified as different chemotypes confirms the opinion that the chemical composition of essential oils sometimes critically depends on the time of collection. Also, for the definition of chemotypes it is not enough to base this on a chemical analysis of an oil from one phenophase only.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "Seasonal Variations in the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis, Lamiaceae)",
volume = "8",
number = "1",
pages = "131-134",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1937"
}

Lakušić, D., Ristić, M., Slavkovska, V.,& Lakušić, B.. (2013). Seasonal Variations in the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis, Lamiaceae). in Natural Product Communications
Natural Products Inc, Westerville., 8(1), 131-134.
https://hdl.handle.net/21.15107/rcub_farfar_1937

Lakušić D, Ristić M, Slavkovska V, Lakušić B. Seasonal Variations in the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis, Lamiaceae). in Natural Product Communications. 2013;8(1):131-134.
https://hdl.handle.net/21.15107/rcub_farfar_1937 .

Lakušić, Dmitar, Ristić, Mihailo, Slavkovska, Violeta, Lakušić, Branislava, "Seasonal Variations in the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis, Lamiaceae)" in Natural Product Communications, 8, no. 1 (2013):131-134,
https://hdl.handle.net/21.15107/rcub_farfar_1937 .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Lakušić, Branislava
AU  - Lakušić, Dmitar
AU  - Kovačević, Nada
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1904
AB  - The chemical compositions of the essential oils of seven natural populations of Seseli rigidum were analyzed. The essential-oil yield ranged from 0.16 to 2.09%. Analysis of variance (ANOVA) revealed that there were no statistically significant differences in the mean essential-oil yields between the populations, and no significant influence of the climate or soil type on the oil yield was observed. In all 67 analyzed samples, the polyacetylene falcarinol was the main compound, followed by octanal, methyl linoleate, -muurolene, 3-butylphthalide, falcarinone, muurola-4,10(14)-dien-1-ol, -sesquiphellandrene, salvial-4(14)-en-1-one, -amorphene, spathulenol, and isospathulenol. The principal component analysis (PCA), the canonical discriminant analysis (CDA), and the cluster analysis (CA) revealed differentiation between the populations based on the climate. Three groups of populations were formed; the first group was composed of samples growing in regions with a humid climate, with oils having high falcarinol and low sesquiterpene contents, and the second and third groups comprised samples exposed to semi-arid climate, with oils characterized by a lower falcarinol and higher -muurolene, -amorphene, -sesquiphellandrene, and salvial-4(14)-en-1-one contents. The semi-arid populations were divided into two groups, which were distinguished based on the oil contents of sesquiterpenes, falcarinone, and 3-butylphthalide. On the other hand, no clear separation between populations based on the different soil types could be observed.
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Chemistry & Biodiversity
T1  - Variability of the Root Essential Oils of Seseli rigidum Waldst. & Kit. (Apiaceae) from Different Populations in Serbia
VL  - 10
IS  - 9
SP  - 1653
EP  - 1666
DO  - 10.1002/cbdv.201200439
ER  - 

@article{
author = "Marčetić, Mirjana and Lakušić, Branislava and Lakušić, Dmitar and Kovačević, Nada",
year = "2013",
abstract = "The chemical compositions of the essential oils of seven natural populations of Seseli rigidum were analyzed. The essential-oil yield ranged from 0.16 to 2.09%. Analysis of variance (ANOVA) revealed that there were no statistically significant differences in the mean essential-oil yields between the populations, and no significant influence of the climate or soil type on the oil yield was observed. In all 67 analyzed samples, the polyacetylene falcarinol was the main compound, followed by octanal, methyl linoleate, -muurolene, 3-butylphthalide, falcarinone, muurola-4,10(14)-dien-1-ol, -sesquiphellandrene, salvial-4(14)-en-1-one, -amorphene, spathulenol, and isospathulenol. The principal component analysis (PCA), the canonical discriminant analysis (CDA), and the cluster analysis (CA) revealed differentiation between the populations based on the climate. Three groups of populations were formed; the first group was composed of samples growing in regions with a humid climate, with oils having high falcarinol and low sesquiterpene contents, and the second and third groups comprised samples exposed to semi-arid climate, with oils characterized by a lower falcarinol and higher -muurolene, -amorphene, -sesquiphellandrene, and salvial-4(14)-en-1-one contents. The semi-arid populations were divided into two groups, which were distinguished based on the oil contents of sesquiterpenes, falcarinone, and 3-butylphthalide. On the other hand, no clear separation between populations based on the different soil types could be observed.",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Variability of the Root Essential Oils of Seseli rigidum Waldst. & Kit. (Apiaceae) from Different Populations in Serbia",
volume = "10",
number = "9",
pages = "1653-1666",
doi = "10.1002/cbdv.201200439"
}

Marčetić, M., Lakušić, B., Lakušić, D.,& Kovačević, N.. (2013). Variability of the Root Essential Oils of Seseli rigidum Waldst. & Kit. (Apiaceae) from Different Populations in Serbia. in Chemistry & Biodiversity
Wiley-VCH Verlag GMBH, Weinheim., 10(9), 1653-1666.
https://doi.org/10.1002/cbdv.201200439

Marčetić M, Lakušić B, Lakušić D, Kovačević N. Variability of the Root Essential Oils of Seseli rigidum Waldst. & Kit. (Apiaceae) from Different Populations in Serbia. in Chemistry & Biodiversity. 2013;10(9):1653-1666.
doi:10.1002/cbdv.201200439 .

Marčetić, Mirjana, Lakušić, Branislava, Lakušić, Dmitar, Kovačević, Nada, "Variability of the Root Essential Oils of Seseli rigidum Waldst. & Kit. (Apiaceae) from Different Populations in Serbia" in Chemistry & Biodiversity, 10, no. 9 (2013):1653-1666,
https://doi.org/10.1002/cbdv.201200439 . .

TY  - JOUR
AU  - Lakušić, Branislava
AU  - Ristić, Mihailo
AU  - Slavkovska, Violeta
AU  - Stojanović, Danilo
AU  - Lakušić, Dmitar
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1880
AB  - Variations in the yield and composition of essential oil of Dalmatian sage (Salvia officinalis L., Lamiaceae) in various stages of development were analyzed in two individuals (= genotypes) of different geographic origin. Both plants have been successfully grown ten years under identical environmental conditions in a private garden in Belgrade. The amount and composition of the oils were analyzed during one growing season starting from the young to old overwintered leaves. The results of the cluster analysis showed that leaf age and origin of the plants has a significant impact on the composition of the essential oils. All oil samples formed two main clades. The first clade are oils collected from young leaves from April to June and belong to α-humulene type. The second clade includes oil obtained mainly of old leaves, which were collected from August to June, and belonging to the camphor and thujone type. In the second clade samples originating from Učka are completely separated from samples originating from Belgrade. Also, based on these analyzes showed that the same individual (the same genotype) during one growing season significantly change the chemical composition of the essential oils. Thus, it is possible to distinguish three different 'phenological types of essential oils': young leaves ('yl-oils'), early old leaves ('early-ol-oils') and a late old leaves type ('late-ol-oils'). Analysis of the seasonal changes in the composition of the essential oils of Dalmatian sage is shown that different components have different dynamics and different directions of change in the concentration during the season.
AB  - Variranje prinosa i sastava etarskog ulja dalmatinske žalfije (Salvia officinalis L., Lamiaceae) u različitim fazama razvoja analizirano je na dve individue (= genotipa) različitog geografskog porekla. Obe biljke su uspešno gajene deset godina pod identičnom ekološkim uslovima u privatnoj bašti u Beogradu. Količina i sastav ulja su analizirani tokom jedne vegetacione sezone počevši od mladih pa sve do starih prezimelih listova. Rezultati klaster analize su pokazali da starost listova kao i poreklo biljke ima veoma značajan uticaj na sastav etarskog ulja. Svi uzorci ulja su formirali dve osnovne klade. Prvu kladu čine ulja mladih listova sakupljanih od aprila do juna i pripadaju α-humulen tipu. Druga klada obuhvata ulja dobijena uglavnom od starih listova, koji su sakupljani od avgusta do juna, i koji pripadaju kamfor ili tujon tipu. U drugoj kladi uzorci poreklom sa Učke su potpuno odvojeni od uzoraka poreklom iz Beograda. Takođe, na osnovu ovih analiza je utvrđeno da ista individua (isti genotip) u toku jedne vegetacione sezone značajno menja hemijski sastav etarskog ulja. Tako je moguće razlikovati tri različita 'fenološka tipa etarskih ulja': ulja mladih listova, ulja rane faze starih listova i ulja kasne faze starih listova. Analiza sezonskih promena u sastavu etarskih ulja Dalmatinske žalfije je pokazala da različite komponente imaju različitu dinamiku i različite pravce promena u koncentraciji tokom sezone.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages
T1  - Variranje prinosa i sastava etarskih ulja dalmatinske žalfije (Salvia officinalis Lamiaceae) u različitim fazama razvoja
VL  - 37
IS  - 2
SP  - 127
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1880
ER  - 

@article{
author = "Lakušić, Branislava and Ristić, Mihailo and Slavkovska, Violeta and Stojanović, Danilo and Lakušić, Dmitar",
year = "2013",
abstract = "Variations in the yield and composition of essential oil of Dalmatian sage (Salvia officinalis L., Lamiaceae) in various stages of development were analyzed in two individuals (= genotypes) of different geographic origin. Both plants have been successfully grown ten years under identical environmental conditions in a private garden in Belgrade. The amount and composition of the oils were analyzed during one growing season starting from the young to old overwintered leaves. The results of the cluster analysis showed that leaf age and origin of the plants has a significant impact on the composition of the essential oils. All oil samples formed two main clades. The first clade are oils collected from young leaves from April to June and belong to α-humulene type. The second clade includes oil obtained mainly of old leaves, which were collected from August to June, and belonging to the camphor and thujone type. In the second clade samples originating from Učka are completely separated from samples originating from Belgrade. Also, based on these analyzes showed that the same individual (the same genotype) during one growing season significantly change the chemical composition of the essential oils. Thus, it is possible to distinguish three different 'phenological types of essential oils': young leaves ('yl-oils'), early old leaves ('early-ol-oils') and a late old leaves type ('late-ol-oils'). Analysis of the seasonal changes in the composition of the essential oils of Dalmatian sage is shown that different components have different dynamics and different directions of change in the concentration during the season., Variranje prinosa i sastava etarskog ulja dalmatinske žalfije (Salvia officinalis L., Lamiaceae) u različitim fazama razvoja analizirano je na dve individue (= genotipa) različitog geografskog porekla. Obe biljke su uspešno gajene deset godina pod identičnom ekološkim uslovima u privatnoj bašti u Beogradu. Količina i sastav ulja su analizirani tokom jedne vegetacione sezone počevši od mladih pa sve do starih prezimelih listova. Rezultati klaster analize su pokazali da starost listova kao i poreklo biljke ima veoma značajan uticaj na sastav etarskog ulja. Svi uzorci ulja su formirali dve osnovne klade. Prvu kladu čine ulja mladih listova sakupljanih od aprila do juna i pripadaju α-humulen tipu. Druga klada obuhvata ulja dobijena uglavnom od starih listova, koji su sakupljani od avgusta do juna, i koji pripadaju kamfor ili tujon tipu. U drugoj kladi uzorci poreklom sa Učke su potpuno odvojeni od uzoraka poreklom iz Beograda. Takođe, na osnovu ovih analiza je utvrđeno da ista individua (isti genotip) u toku jedne vegetacione sezone značajno menja hemijski sastav etarskog ulja. Tako je moguće razlikovati tri različita 'fenološka tipa etarskih ulja': ulja mladih listova, ulja rane faze starih listova i ulja kasne faze starih listova. Analiza sezonskih promena u sastavu etarskih ulja Dalmatinske žalfije je pokazala da različite komponente imaju različitu dinamiku i različite pravce promena u koncentraciji tokom sezone.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages, Variranje prinosa i sastava etarskih ulja dalmatinske žalfije (Salvia officinalis Lamiaceae) u različitim fazama razvoja",
volume = "37",
number = "2",
pages = "127-139",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1880"
}

Lakušić, B., Ristić, M., Slavkovska, V., Stojanović, D.,& Lakušić, D.. (2013). Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages. in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 37(2), 127-139.
https://hdl.handle.net/21.15107/rcub_farfar_1880

Lakušić B, Ristić M, Slavkovska V, Stojanović D, Lakušić D. Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages. in Botanica Serbica. 2013;37(2):127-139.
https://hdl.handle.net/21.15107/rcub_farfar_1880 .

Lakušić, Branislava, Ristić, Mihailo, Slavkovska, Violeta, Stojanović, Danilo, Lakušić, Dmitar, "Variations in essential oil yields and compositions of Salvia officinalis (Lamiaceae) at different developmental stages" in Botanica Serbica, 37, no. 2 (2013):127-139,
https://hdl.handle.net/21.15107/rcub_farfar_1880 .

TY  - JOUR
AU  - Lakušić, Dmitar
AU  - Ristić, Mihailo
AU  - Slavkovska, Violeta
AU  - Sinzar-Sekulić, Jasmina B.
AU  - Lakušić, Branislava
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1738
AB  - Composition of the essential oils of Rosmarinus officinalis of ten populations from the Balkan Peninsula were determined by GC/FID and GC/MS. The main constituents were 1,8-cineole, camphor, a-pinene, and borneol. Multivariate statistical analysis (UPGMA cluster analysis and principal-component analysis (PCA)) revealed two major types of rosemary oil, i.e., 1,8-cineole and camphor-type, and two intermediate types, i.e., camphor/1,8-cineole/borneol type and 1,8-cineole/camphor type. The regression analyses (simple linear regression and stepwise multiple regression) have shown that, with respect to basic geographic, orographic, and 19 bioclimatic characteristics of each population, bioclimatic factor temperature of habitat represented the dominant abiogenetic factor, which, in chemical sense, led to differentiation of populations in the studied region. Also, the regression analysis have shown that some constituents of essential oils are independent of any single bioclimatic factors. However, some constituents display statistically significant correlations with some abiotic factors.
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Chemistry & Biodiversity
T1  - Environment-Related Variations of the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis L.) in the Balkan Penninsula
VL  - 9
IS  - 7
SP  - 1286
EP  - 1302
DO  - 10.1002/cbdv.201100427
ER  - 

@article{
author = "Lakušić, Dmitar and Ristić, Mihailo and Slavkovska, Violeta and Sinzar-Sekulić, Jasmina B. and Lakušić, Branislava",
year = "2012",
abstract = "Composition of the essential oils of Rosmarinus officinalis of ten populations from the Balkan Peninsula were determined by GC/FID and GC/MS. The main constituents were 1,8-cineole, camphor, a-pinene, and borneol. Multivariate statistical analysis (UPGMA cluster analysis and principal-component analysis (PCA)) revealed two major types of rosemary oil, i.e., 1,8-cineole and camphor-type, and two intermediate types, i.e., camphor/1,8-cineole/borneol type and 1,8-cineole/camphor type. The regression analyses (simple linear regression and stepwise multiple regression) have shown that, with respect to basic geographic, orographic, and 19 bioclimatic characteristics of each population, bioclimatic factor temperature of habitat represented the dominant abiogenetic factor, which, in chemical sense, led to differentiation of populations in the studied region. Also, the regression analysis have shown that some constituents of essential oils are independent of any single bioclimatic factors. However, some constituents display statistically significant correlations with some abiotic factors.",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Environment-Related Variations of the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis L.) in the Balkan Penninsula",
volume = "9",
number = "7",
pages = "1286-1302",
doi = "10.1002/cbdv.201100427"
}

Lakušić, D., Ristić, M., Slavkovska, V., Sinzar-Sekulić, J. B.,& Lakušić, B.. (2012). Environment-Related Variations of the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis L.) in the Balkan Penninsula. in Chemistry & Biodiversity
Wiley-VCH Verlag GMBH, Weinheim., 9(7), 1286-1302.
https://doi.org/10.1002/cbdv.201100427

Lakušić D, Ristić M, Slavkovska V, Sinzar-Sekulić JB, Lakušić B. Environment-Related Variations of the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis L.) in the Balkan Penninsula. in Chemistry & Biodiversity. 2012;9(7):1286-1302.
doi:10.1002/cbdv.201100427 .

Lakušić, Dmitar, Ristić, Mihailo, Slavkovska, Violeta, Sinzar-Sekulić, Jasmina B., Lakušić, Branislava, "Environment-Related Variations of the Composition of the Essential Oils of Rosemary (Rosmarinus officinalis L.) in the Balkan Penninsula" in Chemistry & Biodiversity, 9, no. 7 (2012):1286-1302,
https://doi.org/10.1002/cbdv.201100427 . .

TY  - JOUR
AU  - Lakušić, Branislava
AU  - Ristić, Mihailo
AU  - Slavkovska, Violeta
AU  - Milenković, Marina
AU  - Lakušić, Dmitar
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1526
AB  - The chemical composition of the essential oils of Satureja horvatii SILIC from two natural habitats (Mt. Orjen and Mt. Lovcen in Montenegro) and from cultivated plants (Belgrade, Serbia) were characterized. For the latter, plants from the locus classicus, i.e., Orjenske Lokve (Mt. Orjen), were transferred to Belgrade and, after three years of cultivation, the chemical composition of their essential oils at different phenological stages was analyzed. The essential oils were obtained from the aerial parts of the plants by hydrodistillation and analyzed by GC and GC/MS. The yields and chemical compositions of the S. horvatii oils showed significant differences between the plants collected in the natural habitats and those from cultivation, as well as between the plants at different phenological stages. In the populations from the natural habitats, growing in Mediterranean conditions, the most abundant oil constituents were the phenols thymol (63.7% in the samples from Mt. Orjen) or carvacrol (68.1% in the samples from Mt. Lovcen), while the oils from the cultivated plants (Belgrade), growing in continental conditions, were dominated by linalool (up to 65.8 and 55.9% in average). The basic characteristics of the essential oil from plants at the early phenological stage (before flowering) were high percentages of linalool (37.4%), thymol (27.3%), and carvacrol (12.2%). At the stage of flowering, the percentage of linalool (56.6-57.5%) increased, while those of thymol (15.5-15.8%) and carvacrol (1.4-1.5%) significantly decreased. The essential oil of plants in the full stage of fruiting was characterized by the domination of linalool (58.4 and 65.8%) and lower percentages of thymol (7.6 and 1.3%) and carvacrol (0.7 and 0.1%). In conclusion, the oil composition of S. horvatii was found to depend on the pedoclimatic conditions of the habitat and the phenological stage of the plants.
PB  - Wiley-Blackwell, Malden
T2  - Chemistry & Biodiversity
T1  - Environmental and Seasonal Impacts on the Chemical Composition of Satureja horvatii Šilić (Lamiaceae) Essential Oils
VL  - 8
IS  - 3
SP  - 483
EP  - 493
DO  - 10.1002/cbdv.201000169
ER  - 

@article{
author = "Lakušić, Branislava and Ristić, Mihailo and Slavkovska, Violeta and Milenković, Marina and Lakušić, Dmitar",
year = "2011",
abstract = "The chemical composition of the essential oils of Satureja horvatii SILIC from two natural habitats (Mt. Orjen and Mt. Lovcen in Montenegro) and from cultivated plants (Belgrade, Serbia) were characterized. For the latter, plants from the locus classicus, i.e., Orjenske Lokve (Mt. Orjen), were transferred to Belgrade and, after three years of cultivation, the chemical composition of their essential oils at different phenological stages was analyzed. The essential oils were obtained from the aerial parts of the plants by hydrodistillation and analyzed by GC and GC/MS. The yields and chemical compositions of the S. horvatii oils showed significant differences between the plants collected in the natural habitats and those from cultivation, as well as between the plants at different phenological stages. In the populations from the natural habitats, growing in Mediterranean conditions, the most abundant oil constituents were the phenols thymol (63.7% in the samples from Mt. Orjen) or carvacrol (68.1% in the samples from Mt. Lovcen), while the oils from the cultivated plants (Belgrade), growing in continental conditions, were dominated by linalool (up to 65.8 and 55.9% in average). The basic characteristics of the essential oil from plants at the early phenological stage (before flowering) were high percentages of linalool (37.4%), thymol (27.3%), and carvacrol (12.2%). At the stage of flowering, the percentage of linalool (56.6-57.5%) increased, while those of thymol (15.5-15.8%) and carvacrol (1.4-1.5%) significantly decreased. The essential oil of plants in the full stage of fruiting was characterized by the domination of linalool (58.4 and 65.8%) and lower percentages of thymol (7.6 and 1.3%) and carvacrol (0.7 and 0.1%). In conclusion, the oil composition of S. horvatii was found to depend on the pedoclimatic conditions of the habitat and the phenological stage of the plants.",
publisher = "Wiley-Blackwell, Malden",
journal = "Chemistry & Biodiversity",
title = "Environmental and Seasonal Impacts on the Chemical Composition of Satureja horvatii Šilić (Lamiaceae) Essential Oils",
volume = "8",
number = "3",
pages = "483-493",
doi = "10.1002/cbdv.201000169"
}

Lakušić, B., Ristić, M., Slavkovska, V., Milenković, M.,& Lakušić, D.. (2011). Environmental and Seasonal Impacts on the Chemical Composition of Satureja horvatii Šilić (Lamiaceae) Essential Oils. in Chemistry & Biodiversity
Wiley-Blackwell, Malden., 8(3), 483-493.
https://doi.org/10.1002/cbdv.201000169

Lakušić B, Ristić M, Slavkovska V, Milenković M, Lakušić D. Environmental and Seasonal Impacts on the Chemical Composition of Satureja horvatii Šilić (Lamiaceae) Essential Oils. in Chemistry & Biodiversity. 2011;8(3):483-493.
doi:10.1002/cbdv.201000169 .

Lakušić, Branislava, Ristić, Mihailo, Slavkovska, Violeta, Milenković, Marina, Lakušić, Dmitar, "Environmental and Seasonal Impacts on the Chemical Composition of Satureja horvatii Šilić (Lamiaceae) Essential Oils" in Chemistry & Biodiversity, 8, no. 3 (2011):483-493,
https://doi.org/10.1002/cbdv.201000169 . .

TY  - JOUR
AU  - Lakušić, Branislava
AU  - Lakušić, Dmitar
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1453
AB  - The aim of this research was to describe variability of anatomical characteristics of needles and stems of the taxa: J. communis subsp. communis var. communis, J. communis subsp. communis var. intermedia Sanio, J. communis subsp. alpina (Suter) Čelak and J. deltoides Adams, from Serbia, Montenegro and Macedonia (Balkan Peninsula), to establish whether anatomical differences are influenced by the phylogenetic or ecological position of individual taxa. Macroscopic and microscopic analyses were done on samples of 13 populations from Eu-Mediterranean coastal areas of up to 2,000 m a.s.l. in continental mountains. Descriptive statistics were calculated for 23 quantitative characters. Canonical Discriminant Analysis (CDA) and clustering on the basis of the UPGMA method were performed to measure distances between the groups. At an anatomical level there was fine differentiation between the taxa, at the level of species and subspecies. The species J. communis and J. deltoides differed in number of stomatal bands, needle cross-sectional shape, dimensions of the needle resin duct, as well as in the structure of the central cylinder in the primary structure of the stem. Within the species J. communis, high mountain subspecies J. communis subsp. alpina was fi nely differentiated compared with the typical subspecies J. communis subsp. communis. Basic differences between these two taxa were reflected in the shape and dimensions of the needles. Discriminant and cluster analyses did not show any major differences between J. communis subsp. communis var. communis and J. communis subsp. communis var. intermedia. Multivariate analysis showed that the level of anatomical differentiation of these taxa were conditioned partially by phylogenetic association of individual taxa, and partially by ecological conditions of the habitat. Our results showed that anatomical characteristics of needle and primary structures of the stem of these species of genus Juniperus have taxonomic significance at the species and infraspecies levels.
AB  - U radu je opisana varijabilnost anatomskih karaktera četina i primarne i sekundarne građe stabla taksona: J. communis subsp. communis var. communis, J. communis subsp. communis var. intermedia Sanio, J. communis subsp. alpina (Suter) Čelak i J. deltoides Adams (= J. oxycedrus auct. Apen., Balk. & Turk., non L.), sa prostora Balkanskog poluostrva - Srbije, Crne Gore i Makedonije Makroskopska i mikroskopska istraživanja, koja su urađena na biljkama iz 13 eumediteranskih i kontinentalnih populacija, ukazuju da na anatomskom nivou između istraživanih taksona postoji fina izdiferenciranost na nivou vrsta i podvrsta. Vrste J. communis i J. deltoides se na anatomskom nivou potpuno razdvajaju po broju linija stoma, obliku poprečnog preseka četine, dimenziji smonog kanala u četini, kao i na osnovu građe centralnog cilindra u primarnoj građi stabla. Unutar vrste J. communis takođe je uočeno da se visokoplaninska J. communis subsp. alpina fi no diferencira u odnosu na tipsku podvrstu J. communis subsp. communis. Osnovne razlike između ova dva taksona su u obliku i dimenzijama četine. Diskriminantna i klaster analiza nisu pokazale značajne razlike između varijeteta J. communis subsp. communis var. communis i J. communis subsp. communis var. intermedia.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Anatomy of four taxa of the genus Juniperus sect: Juniperus (Cupressaceae) from the Balkan peninsula
T1  - Anatomija četiri taksona roda Juniperus L. sect - Juniperus (Cupressaecae) sa Balkanskog poluostrva
VL  - 35
IS  - 2
SP  - 145
EP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1453
ER  - 

@article{
author = "Lakušić, Branislava and Lakušić, Dmitar",
year = "2011",
abstract = "The aim of this research was to describe variability of anatomical characteristics of needles and stems of the taxa: J. communis subsp. communis var. communis, J. communis subsp. communis var. intermedia Sanio, J. communis subsp. alpina (Suter) Čelak and J. deltoides Adams, from Serbia, Montenegro and Macedonia (Balkan Peninsula), to establish whether anatomical differences are influenced by the phylogenetic or ecological position of individual taxa. Macroscopic and microscopic analyses were done on samples of 13 populations from Eu-Mediterranean coastal areas of up to 2,000 m a.s.l. in continental mountains. Descriptive statistics were calculated for 23 quantitative characters. Canonical Discriminant Analysis (CDA) and clustering on the basis of the UPGMA method were performed to measure distances between the groups. At an anatomical level there was fine differentiation between the taxa, at the level of species and subspecies. The species J. communis and J. deltoides differed in number of stomatal bands, needle cross-sectional shape, dimensions of the needle resin duct, as well as in the structure of the central cylinder in the primary structure of the stem. Within the species J. communis, high mountain subspecies J. communis subsp. alpina was fi nely differentiated compared with the typical subspecies J. communis subsp. communis. Basic differences between these two taxa were reflected in the shape and dimensions of the needles. Discriminant and cluster analyses did not show any major differences between J. communis subsp. communis var. communis and J. communis subsp. communis var. intermedia. Multivariate analysis showed that the level of anatomical differentiation of these taxa were conditioned partially by phylogenetic association of individual taxa, and partially by ecological conditions of the habitat. Our results showed that anatomical characteristics of needle and primary structures of the stem of these species of genus Juniperus have taxonomic significance at the species and infraspecies levels., U radu je opisana varijabilnost anatomskih karaktera četina i primarne i sekundarne građe stabla taksona: J. communis subsp. communis var. communis, J. communis subsp. communis var. intermedia Sanio, J. communis subsp. alpina (Suter) Čelak i J. deltoides Adams (= J. oxycedrus auct. Apen., Balk. & Turk., non L.), sa prostora Balkanskog poluostrva - Srbije, Crne Gore i Makedonije Makroskopska i mikroskopska istraživanja, koja su urađena na biljkama iz 13 eumediteranskih i kontinentalnih populacija, ukazuju da na anatomskom nivou između istraživanih taksona postoji fina izdiferenciranost na nivou vrsta i podvrsta. Vrste J. communis i J. deltoides se na anatomskom nivou potpuno razdvajaju po broju linija stoma, obliku poprečnog preseka četine, dimenziji smonog kanala u četini, kao i na osnovu građe centralnog cilindra u primarnoj građi stabla. Unutar vrste J. communis takođe je uočeno da se visokoplaninska J. communis subsp. alpina fi no diferencira u odnosu na tipsku podvrstu J. communis subsp. communis. Osnovne razlike između ova dva taksona su u obliku i dimenzijama četine. Diskriminantna i klaster analiza nisu pokazale značajne razlike između varijeteta J. communis subsp. communis var. communis i J. communis subsp. communis var. intermedia.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Anatomy of four taxa of the genus Juniperus sect: Juniperus (Cupressaceae) from the Balkan peninsula, Anatomija četiri taksona roda Juniperus L. sect - Juniperus (Cupressaecae) sa Balkanskog poluostrva",
volume = "35",
number = "2",
pages = "145-156",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1453"
}

Lakušić, B.,& Lakušić, D.. (2011). Anatomy of four taxa of the genus Juniperus sect: Juniperus (Cupressaceae) from the Balkan peninsula. in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 35(2), 145-156.
https://hdl.handle.net/21.15107/rcub_farfar_1453

Lakušić B, Lakušić D. Anatomy of four taxa of the genus Juniperus sect: Juniperus (Cupressaceae) from the Balkan peninsula. in Botanica Serbica. 2011;35(2):145-156.
https://hdl.handle.net/21.15107/rcub_farfar_1453 .

Lakušić, Branislava, Lakušić, Dmitar, "Anatomy of four taxa of the genus Juniperus sect: Juniperus (Cupressaceae) from the Balkan peninsula" in Botanica Serbica, 35, no. 2 (2011):145-156,
https://hdl.handle.net/21.15107/rcub_farfar_1453 .

TY  - JOUR
AU  - Drobac, Milica
AU  - Lakušić, Dmitar
AU  - Kovačević, Nada
AU  - Tzakou, Olga
AU  - Couladis, Maria
PY  - 2010
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1414
AB  - The essential-oil composition of six Anthemis taxa from several populations in Serbia and Montenegro, Anthemis triumfetti (L.) DC., A. tinctoria L.. A. austriaca JACQ., A. ruthenica BIER., A. COMM L., and A. cretica ssp. carpatica (WILLD.) GRIERSON were analyzed by GC and GC/MS. The main constituents have been identified as follows: camphor (13.8-15.4%), alpha-pinene (5.2-9.3%). beta-pinene (4.9-7.8%), and (E)-caryophyllene (7.3-9.8%) in three populations, and cis-chrysanthenol (27.0%) and 1,8-cineole (8.4%) in one population of A. triumfetti; 1,8-cineole (9.0-25.8%) in the oils of five populations, and borneol (16.0%) and spatulenol (16.0%) in the oil of one population of A. tinctoria: cis-chrysanthenyl acetate (17.5-22.0%). beta-pinene (8.6-13.2%), and 1,8-cineole (7.2-10.4%) in the oils of A. austriaca: germacrene D (8.3-11.3%) and terpinen-4-ol (6.3-7.3%) in A. ruthenica oils; beta-cedrene (10.3-19.0%), (E)-beta-farnesene (78-13.5%), and germacrene D (5.2-9.1%) in the oils of A. cotula; cis-thujone (39.0%), trans-thujone (13.5%), and yomogi alcohol (7.1%) in the oil of A. cretica ssp. carpatica. The essential oil of A. austriaca was studied for the first time. A cluster analysis based on the relative percentages of all components of the essential oils was used to determine the distances between taxa and populations.
PB  - Wiley-Blackwell, Malden
T2  - Chemistry & Biodiversity
T1  - Comparative Analysis of Essential Oils of Six Anthemis Taxa from Serbia and Montenegro
VL  - 7
IS  - 5
SP  - 1231
EP  - 1244
DO  - 10.1002/cbdv.200900156
ER  - 

@article{
author = "Drobac, Milica and Lakušić, Dmitar and Kovačević, Nada and Tzakou, Olga and Couladis, Maria",
year = "2010",
abstract = "The essential-oil composition of six Anthemis taxa from several populations in Serbia and Montenegro, Anthemis triumfetti (L.) DC., A. tinctoria L.. A. austriaca JACQ., A. ruthenica BIER., A. COMM L., and A. cretica ssp. carpatica (WILLD.) GRIERSON were analyzed by GC and GC/MS. The main constituents have been identified as follows: camphor (13.8-15.4%), alpha-pinene (5.2-9.3%). beta-pinene (4.9-7.8%), and (E)-caryophyllene (7.3-9.8%) in three populations, and cis-chrysanthenol (27.0%) and 1,8-cineole (8.4%) in one population of A. triumfetti; 1,8-cineole (9.0-25.8%) in the oils of five populations, and borneol (16.0%) and spatulenol (16.0%) in the oil of one population of A. tinctoria: cis-chrysanthenyl acetate (17.5-22.0%). beta-pinene (8.6-13.2%), and 1,8-cineole (7.2-10.4%) in the oils of A. austriaca: germacrene D (8.3-11.3%) and terpinen-4-ol (6.3-7.3%) in A. ruthenica oils; beta-cedrene (10.3-19.0%), (E)-beta-farnesene (78-13.5%), and germacrene D (5.2-9.1%) in the oils of A. cotula; cis-thujone (39.0%), trans-thujone (13.5%), and yomogi alcohol (7.1%) in the oil of A. cretica ssp. carpatica. The essential oil of A. austriaca was studied for the first time. A cluster analysis based on the relative percentages of all components of the essential oils was used to determine the distances between taxa and populations.",
publisher = "Wiley-Blackwell, Malden",
journal = "Chemistry & Biodiversity",
title = "Comparative Analysis of Essential Oils of Six Anthemis Taxa from Serbia and Montenegro",
volume = "7",
number = "5",
pages = "1231-1244",
doi = "10.1002/cbdv.200900156"
}

Drobac, M., Lakušić, D., Kovačević, N., Tzakou, O.,& Couladis, M.. (2010). Comparative Analysis of Essential Oils of Six Anthemis Taxa from Serbia and Montenegro. in Chemistry & Biodiversity
Wiley-Blackwell, Malden., 7(5), 1231-1244.
https://doi.org/10.1002/cbdv.200900156

Drobac M, Lakušić D, Kovačević N, Tzakou O, Couladis M. Comparative Analysis of Essential Oils of Six Anthemis Taxa from Serbia and Montenegro. in Chemistry & Biodiversity. 2010;7(5):1231-1244.
doi:10.1002/cbdv.200900156 .

Drobac, Milica, Lakušić, Dmitar, Kovačević, Nada, Tzakou, Olga, Couladis, Maria, "Comparative Analysis of Essential Oils of Six Anthemis Taxa from Serbia and Montenegro" in Chemistry & Biodiversity, 7, no. 5 (2010):1231-1244,
https://doi.org/10.1002/cbdv.200900156 . .