TY  - JOUR
AU  - Foivas, Anargyros
AU  - Malenović, Anđelija
AU  - Kostić, Nada
AU  - Božić, Marija
AU  - Knežević, Miroslav
AU  - Loukas, Yannis L.
AU  - Dotsikas, Yannis
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2566
AB  - In the current study, a rapid and sensitive LC-QTOF-MS/MS method for the determination of brinzolamide in dried blood spots (DBS) was developed and validated. This novel sample collection, storage and transfer technique was suitable for analyzing a drug with high distribution into red blood cells and negligible plasma levels. The method included an isocratic mobile phase consisting of methanol and 10 mM ammonium formate (90:10, v/v) and detection in positive electrospray mode (ESI+). The flow rate was adjusted to 0.350 mL/min yielding retention times of 1.7 min for both brinzolamide and internal standard (IS) rabeprazole on a Cyano analytical column, respectively. The validation of the proposed method over the concentration range 0.500-20.0 mu g/mL was performed in compliance with EMEA and FDA guidelines, assessing all major performance characteristics. Inter- and intra- assay precisions were less than 14%, while inter- and intra- assay accuracies varied from 922 to 111%. No matrix effect was observed and the mean brinzolamide extraction recovery was 93.5%. The method was successfully applied to real DBS samples from patients in steady state condition, receiving brinzolamide ophthalmic suspension 1% (w/v) for several months. Initial concentrations were corrected due to hematocrit effect, using image processing algorithm written in Matlab.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method
VL  - 119
SP  - 84
EP  - 90
DO  - 10.1016/j.jpba.2015.11.043
ER  - 

@article{
author = "Foivas, Anargyros and Malenović, Anđelija and Kostić, Nada and Božić, Marija and Knežević, Miroslav and Loukas, Yannis L. and Dotsikas, Yannis",
year = "2016",
abstract = "In the current study, a rapid and sensitive LC-QTOF-MS/MS method for the determination of brinzolamide in dried blood spots (DBS) was developed and validated. This novel sample collection, storage and transfer technique was suitable for analyzing a drug with high distribution into red blood cells and negligible plasma levels. The method included an isocratic mobile phase consisting of methanol and 10 mM ammonium formate (90:10, v/v) and detection in positive electrospray mode (ESI+). The flow rate was adjusted to 0.350 mL/min yielding retention times of 1.7 min for both brinzolamide and internal standard (IS) rabeprazole on a Cyano analytical column, respectively. The validation of the proposed method over the concentration range 0.500-20.0 mu g/mL was performed in compliance with EMEA and FDA guidelines, assessing all major performance characteristics. Inter- and intra- assay precisions were less than 14%, while inter- and intra- assay accuracies varied from 922 to 111%. No matrix effect was observed and the mean brinzolamide extraction recovery was 93.5%. The method was successfully applied to real DBS samples from patients in steady state condition, receiving brinzolamide ophthalmic suspension 1% (w/v) for several months. Initial concentrations were corrected due to hematocrit effect, using image processing algorithm written in Matlab.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method",
volume = "119",
pages = "84-90",
doi = "10.1016/j.jpba.2015.11.043"
}

Foivas, A., Malenović, A., Kostić, N., Božić, M., Knežević, M., Loukas, Y. L.,& Dotsikas, Y.. (2016). Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier Science BV, Amsterdam., 119, 84-90.
https://doi.org/10.1016/j.jpba.2015.11.043

Foivas A, Malenović A, Kostić N, Božić M, Knežević M, Loukas YL, Dotsikas Y. Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method. in Journal of Pharmaceutical and Biomedical Analysis. 2016;119:84-90.
doi:10.1016/j.jpba.2015.11.043 .

Foivas, Anargyros, Malenović, Anđelija, Kostić, Nada, Božić, Marija, Knežević, Miroslav, Loukas, Yannis L., Dotsikas, Yannis, "Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method" in Journal of Pharmaceutical and Biomedical Analysis, 119 (2016):84-90,
https://doi.org/10.1016/j.jpba.2015.11.043 . .