TY  - JOUR
AU  - Vladimirov, Sandra
AU  - Gojković, Tamara
AU  - Zeljković, Aleksandra
AU  - Jelić-Ivanović, Zorana
AU  - Zeljković, Dejan
AU  - Antonić, Tamara
AU  - Trifunović, Bratislav
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4178
AB  - Colorectal cancer (CRC) is a highly prevalent malignancy. Previous studies suggested that cholesterol might play a signficant role in malignant transformation and proliferation. Non-cholesterol sterols (NCS), which are transported by serum lipoproteins alongside cholesterol, are regarded as cholesterol synthesis and absorption markers. Quantification of NCS in serum and HDL fraction (NCSHDL), could provide a better insight into the cholesterol metabolism. The aim of this study was to examine the status of cholesterol synthesis and cholesterol absorption markers in serum and HDL fraction and explore their interrelation in CRC patients. Current study was designed as observational, case-control study. The study included 73 CRC patients and 95 healthy subjects. NCS and NCSHDL concentrations were determined by HPLC-MS/MS. Based on NCS and NCSHDL concentrations, different cholesterol homeostasis indices were calculated. Patients had significantly lower NCS (P<0.001) and NCSHDL concentrations (P<0.001 for desmosterolHDL; P<0.05 for lathosterolHDL, P=0.001 for campesterolHDL, P<0.001 for β-sitosterolHDL). NCSHDL/NCS (P<0.005 for desmosterolHDL/desmosterol; P<0.05 for lathosterolHDL/lathosterol; P<0.001 for both β-sitosterolHDL/β-sitosterol and campesterolHDL/campesterol) and synthesis to absorption ratio (CSI/CAI) (P<0.005) were increased in CRC patients. Additionally, low serum concentrations of desmosterol (P<0.001; OR=0.329; 95%CI (0.199–0.542)) and campesterol (P<0.001; OR=0.540; 95%CI (0.424–0.687)) were independent predictors of CRC presence. Our data suggest that cholesterol homeostasis in CRC is shifted towards increased synthesis. Relative abundance of NCS in HDL particles is increased, suggesting the possible overproduction of cholesterol precursors in peripheral tissues.
PB  - Elsevier Inc.
T2  - Biochemical Pharmacology
T1  - Can non-cholesterol sterols indicate the presence of specific dysregulation of cholesterol metabolism in patients with colorectal cancer?
VL  - 196
DO  - 10.1016/j.bcp.2021.114595
ER  - 

@article{
author = "Vladimirov, Sandra and Gojković, Tamara and Zeljković, Aleksandra and Jelić-Ivanović, Zorana and Zeljković, Dejan and Antonić, Tamara and Trifunović, Bratislav and Spasojević-Kalimanovska, Vesna",
year = "2022",
abstract = "Colorectal cancer (CRC) is a highly prevalent malignancy. Previous studies suggested that cholesterol might play a signficant role in malignant transformation and proliferation. Non-cholesterol sterols (NCS), which are transported by serum lipoproteins alongside cholesterol, are regarded as cholesterol synthesis and absorption markers. Quantification of NCS in serum and HDL fraction (NCSHDL), could provide a better insight into the cholesterol metabolism. The aim of this study was to examine the status of cholesterol synthesis and cholesterol absorption markers in serum and HDL fraction and explore their interrelation in CRC patients. Current study was designed as observational, case-control study. The study included 73 CRC patients and 95 healthy subjects. NCS and NCSHDL concentrations were determined by HPLC-MS/MS. Based on NCS and NCSHDL concentrations, different cholesterol homeostasis indices were calculated. Patients had significantly lower NCS (P<0.001) and NCSHDL concentrations (P<0.001 for desmosterolHDL; P<0.05 for lathosterolHDL, P=0.001 for campesterolHDL, P<0.001 for β-sitosterolHDL). NCSHDL/NCS (P<0.005 for desmosterolHDL/desmosterol; P<0.05 for lathosterolHDL/lathosterol; P<0.001 for both β-sitosterolHDL/β-sitosterol and campesterolHDL/campesterol) and synthesis to absorption ratio (CSI/CAI) (P<0.005) were increased in CRC patients. Additionally, low serum concentrations of desmosterol (P<0.001; OR=0.329; 95%CI (0.199–0.542)) and campesterol (P<0.001; OR=0.540; 95%CI (0.424–0.687)) were independent predictors of CRC presence. Our data suggest that cholesterol homeostasis in CRC is shifted towards increased synthesis. Relative abundance of NCS in HDL particles is increased, suggesting the possible overproduction of cholesterol precursors in peripheral tissues.",
publisher = "Elsevier Inc.",
journal = "Biochemical Pharmacology",
title = "Can non-cholesterol sterols indicate the presence of specific dysregulation of cholesterol metabolism in patients with colorectal cancer?",
volume = "196",
doi = "10.1016/j.bcp.2021.114595"
}

Vladimirov, S., Gojković, T., Zeljković, A., Jelić-Ivanović, Z., Zeljković, D., Antonić, T., Trifunović, B.,& Spasojević-Kalimanovska, V.. (2022). Can non-cholesterol sterols indicate the presence of specific dysregulation of cholesterol metabolism in patients with colorectal cancer?. in Biochemical Pharmacology
Elsevier Inc.., 196.
https://doi.org/10.1016/j.bcp.2021.114595

Vladimirov S, Gojković T, Zeljković A, Jelić-Ivanović Z, Zeljković D, Antonić T, Trifunović B, Spasojević-Kalimanovska V. Can non-cholesterol sterols indicate the presence of specific dysregulation of cholesterol metabolism in patients with colorectal cancer?. in Biochemical Pharmacology. 2022;196.
doi:10.1016/j.bcp.2021.114595 .

Vladimirov, Sandra, Gojković, Tamara, Zeljković, Aleksandra, Jelić-Ivanović, Zorana, Zeljković, Dejan, Antonić, Tamara, Trifunović, Bratislav, Spasojević-Kalimanovska, Vesna, "Can non-cholesterol sterols indicate the presence of specific dysregulation of cholesterol metabolism in patients with colorectal cancer?" in Biochemical Pharmacology, 196 (2022),
https://doi.org/10.1016/j.bcp.2021.114595 . .

TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Mihajlović, Marija
AU  - Gojković, Tamara
AU  - Vladimirov, Sandra
AU  - Zeljković, Dejan
AU  - Spasojević-Kalimanovska, Vesna
AU  - Trifunović, Bratislav
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3813
AB  - Colorectal cancer (CRC) is a highly prevalent malignancy with multifactorial etiology,which includes metabolic alterations as contributors to disease development. Studies have shownthat lipid status disorders are involved in colorectal carcinogenesis. In line with this, previous studieshave also suggested that the serum high-density lipoprotein cholesterol (HDL-C) level decreases inpatients with CRC, but more recently, the focus of investigations has shifted toward the explorationof qualitative properties of HDL in this malignancy. Herein, a comprehensive overview of availableevidences regarding the putative role of HDL in CRC will be presented. We will analyze existingfindings regarding alterations of HDL-C levels but also HDL particle structure and distribution inCRC. In addition, changes in HDL functionality in this malignancy will be discussed.  Moreover,we will focus on the genetic regulation of HDL metabolism, as well as the involvement of HDL indisturbances of cholesterol trafficking in CRC. Finally, possible therapeutic implications related toHDL will be presented. Given the available evidence, future studies are needed to resolve all raisedissues concerning the suggested protective role of HDL in CRC, its presumed function as a biomarker,and eventual therapeutic approaches based on HDL.
PB  - MDPI AG
T2  - International Journal of Molecular Sciences
T1  - Revealing the role of high-density lipoprotein in colorectal cancer
VL  - 22
IS  - 7
DO  - 10.3390/ijms22073352
ER  - 

@article{
author = "Zeljković, Aleksandra and Vekić, Jelena and Mihajlović, Marija and Gojković, Tamara and Vladimirov, Sandra and Zeljković, Dejan and Spasojević-Kalimanovska, Vesna and Trifunović, Bratislav",
year = "2021",
abstract = "Colorectal cancer (CRC) is a highly prevalent malignancy with multifactorial etiology,which includes metabolic alterations as contributors to disease development. Studies have shownthat lipid status disorders are involved in colorectal carcinogenesis. In line with this, previous studieshave also suggested that the serum high-density lipoprotein cholesterol (HDL-C) level decreases inpatients with CRC, but more recently, the focus of investigations has shifted toward the explorationof qualitative properties of HDL in this malignancy. Herein, a comprehensive overview of availableevidences regarding the putative role of HDL in CRC will be presented. We will analyze existingfindings regarding alterations of HDL-C levels but also HDL particle structure and distribution inCRC. In addition, changes in HDL functionality in this malignancy will be discussed.  Moreover,we will focus on the genetic regulation of HDL metabolism, as well as the involvement of HDL indisturbances of cholesterol trafficking in CRC. Finally, possible therapeutic implications related toHDL will be presented. Given the available evidence, future studies are needed to resolve all raisedissues concerning the suggested protective role of HDL in CRC, its presumed function as a biomarker,and eventual therapeutic approaches based on HDL.",
publisher = "MDPI AG",
journal = "International Journal of Molecular Sciences",
title = "Revealing the role of high-density lipoprotein in colorectal cancer",
volume = "22",
number = "7",
doi = "10.3390/ijms22073352"
}

Zeljković, A., Vekić, J., Mihajlović, M., Gojković, T., Vladimirov, S., Zeljković, D., Spasojević-Kalimanovska, V.,& Trifunović, B.. (2021). Revealing the role of high-density lipoprotein in colorectal cancer. in International Journal of Molecular Sciences
MDPI AG., 22(7).
https://doi.org/10.3390/ijms22073352

Zeljković A, Vekić J, Mihajlović M, Gojković T, Vladimirov S, Zeljković D, Spasojević-Kalimanovska V, Trifunović B. Revealing the role of high-density lipoprotein in colorectal cancer. in International Journal of Molecular Sciences. 2021;22(7).
doi:10.3390/ijms22073352 .

Zeljković, Aleksandra, Vekić, Jelena, Mihajlović, Marija, Gojković, Tamara, Vladimirov, Sandra, Zeljković, Dejan, Spasojević-Kalimanovska, Vesna, Trifunović, Bratislav, "Revealing the role of high-density lipoprotein in colorectal cancer" in International Journal of Molecular Sciences, 22, no. 7 (2021),
https://doi.org/10.3390/ijms22073352 . .

TY  - JOUR
AU  - Vladimirov, Sandra
AU  - Zeljković, Aleksandra
AU  - Gojković, Tamara
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Zeljković, Dejan
AU  - Trifunović, Bratislav
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3623
AB  - Background:Vitamin D deficiency is repeatedly reported in colorectal  cancer  (CRC).  Since  cholesterol  and  vitamin  D share common precursor 7-dehydrocholesterol (7-DHC), it would be important to explore the associations of key vitamin D metabolites and serum lipid parameters in patients with high and low grade CRC. The aim of this study was toanalyze    relationships    between    serum    25(OH)D3,24,25(OH)2D3  and  7-DHC  levels  and  serum  lipids  inpatients with CRC, and to evaluate their potential for prediction of risk for development of high grade CRC. Methods:We  recruited  82  patients  CRC  and  77  controls.7-DHC, 25(OH)D3 and 24,25(OH)2D3 were quantified by LC-MS/MS methods.Results:7-DHC, 25(OH)D3 and vitamin D metabolic ratio(VDMR)  were  significantly  lower  in  CRC  patients  than  in control   group   (P<0.001,   P<0.010,   P<0.050   and P<0.050,  respectively).  25(OH)D3  levels  were  higher  inpatients  with  grade  I  CRC  when  compared  to  grade  II(P<0.050). All vitamin D metabolites positively correlated with  total  cholesterol  (TC)  concentration  in  CRC  patients.25(OH)D3 was significant predictor of increased CRC risk(P<0.010).   After   adjustment   for   TC   concentration,25(OH)D3 lost its predictive abilities. However, 25(OH)D3 remained significant predictor of poorly differentiated type of cancer (P<0.050). Conclusions:We  found  significant  positive  association between  vitamin  D  status  and  serum  total  cholesterol.Although low 25(OH)D3 was found to be a significant risk factor for CRC development, the obtained results primarily suggest profound impact of cholesterol level on vitamin D status  in  CRC.  However,  our  results  suggest  that  low 25(OH)D3 might independently contribute to development of poorly differentiated tumor.
AB  - Uvod: Deficijencija vitamina D je učestalo javlja kod obolelih od kolorektalnog karcinoma (CRC). Kako holesterol i vitamin D dele zajednički prekursor 7-dehidroholesterol (7-DHC), bilo bi značajno ispitati povezanost ključnih metabolita vitamin D i serumskih lipidnih parametara kod pacijenata sa CRC klasifikovanih na osnovu gradusa. U ovom radu ispitivali smo odnos između serumskih koncentracija 25(OH)D3, 24,25(OH)2D3 and 7-DHC i serumskih lipida kod pacijenata sa CRC, kao i doprinos metabolita vitamina D u predikciji rizika za nastanak CRC visokog gradusa. Metode: U studiji su učestvovala 82 pacijenta sa CRC i 77 zdravih ispitanika. 7-DHC, 25(OH)D3 i 24,25(OH)2D3 su kvantifikovani pomoću metode LC-MS/MS. Rezultati: 7-DHC, 25(OH)D3 i odnos metabolita vitamina D (VDMR) su bili značajno niži kod pacijenata sa CRC u odnosu na kontrolnu grupu (P<0,001, P<0,010, P<0,050 i P<0,050, redom). Koncentracije 25(OH)D3 su bile više kod pacijenata sa gradusom I u odnosu na pacijente sa gradusom II (P<0,050). Svi metaboliti vitamina D su pozitivno korelirali sa ukupnim holesterolom kod pacijenata sa CRC. 25(OH)D3 je bio značajan prediktor povećanog rizika za nastanak CRC (P<0,010). Nakon korekcije za koncentraciju ukupnog holesterola, 25(OH)D3 je izgubio prediktivni značaj. Međutim, 25(OH)D3 je ostao značajan prediktor slabo diferenciranog karcinoma (P<0,050). Zaključak: Utvrđena je značajna pozitivna korelacija iz među statusa vitamina D i serumskih koncentracija ukupnog holesterola. Iako je uočeno da je nizak 25(OH)D3 značajan faktor rizika za razvoj CRC, naši rezultati upućuju na dominantan uticaj holesterola na status vitamina D kod ovih pacijenata. Ipak, uočeno je da niski nivoi 25(OH)D3 mogu nezavisno doprineti razvoju slabo diferentovanih oblika CRC.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer
T1  - Povezanost metabolita holesterola i vitamina D sa rizikom za nastanak kolorektalnog karcinoma visokog gradusa
VL  - 39
IS  - 3
SP  - 318
EP  - 327
DO  - 10.2478/jomb-2019-0047
ER  - 

@article{
author = "Vladimirov, Sandra and Zeljković, Aleksandra and Gojković, Tamara and Miljković, Milica and Stefanović, Aleksandra and Zeljković, Dejan and Trifunović, Bratislav and Spasojević-Kalimanovska, Vesna",
year = "2020",
abstract = "Background:Vitamin D deficiency is repeatedly reported in colorectal  cancer  (CRC).  Since  cholesterol  and  vitamin  D share common precursor 7-dehydrocholesterol (7-DHC), it would be important to explore the associations of key vitamin D metabolites and serum lipid parameters in patients with high and low grade CRC. The aim of this study was toanalyze    relationships    between    serum    25(OH)D3,24,25(OH)2D3  and  7-DHC  levels  and  serum  lipids  inpatients with CRC, and to evaluate their potential for prediction of risk for development of high grade CRC. Methods:We  recruited  82  patients  CRC  and  77  controls.7-DHC, 25(OH)D3 and 24,25(OH)2D3 were quantified by LC-MS/MS methods.Results:7-DHC, 25(OH)D3 and vitamin D metabolic ratio(VDMR)  were  significantly  lower  in  CRC  patients  than  in control   group   (P<0.001,   P<0.010,   P<0.050   and P<0.050,  respectively).  25(OH)D3  levels  were  higher  inpatients  with  grade  I  CRC  when  compared  to  grade  II(P<0.050). All vitamin D metabolites positively correlated with  total  cholesterol  (TC)  concentration  in  CRC  patients.25(OH)D3 was significant predictor of increased CRC risk(P<0.010).   After   adjustment   for   TC   concentration,25(OH)D3 lost its predictive abilities. However, 25(OH)D3 remained significant predictor of poorly differentiated type of cancer (P<0.050). Conclusions:We  found  significant  positive  association between  vitamin  D  status  and  serum  total  cholesterol.Although low 25(OH)D3 was found to be a significant risk factor for CRC development, the obtained results primarily suggest profound impact of cholesterol level on vitamin D status  in  CRC.  However,  our  results  suggest  that  low 25(OH)D3 might independently contribute to development of poorly differentiated tumor., Uvod: Deficijencija vitamina D je učestalo javlja kod obolelih od kolorektalnog karcinoma (CRC). Kako holesterol i vitamin D dele zajednički prekursor 7-dehidroholesterol (7-DHC), bilo bi značajno ispitati povezanost ključnih metabolita vitamin D i serumskih lipidnih parametara kod pacijenata sa CRC klasifikovanih na osnovu gradusa. U ovom radu ispitivali smo odnos između serumskih koncentracija 25(OH)D3, 24,25(OH)2D3 and 7-DHC i serumskih lipida kod pacijenata sa CRC, kao i doprinos metabolita vitamina D u predikciji rizika za nastanak CRC visokog gradusa. Metode: U studiji su učestvovala 82 pacijenta sa CRC i 77 zdravih ispitanika. 7-DHC, 25(OH)D3 i 24,25(OH)2D3 su kvantifikovani pomoću metode LC-MS/MS. Rezultati: 7-DHC, 25(OH)D3 i odnos metabolita vitamina D (VDMR) su bili značajno niži kod pacijenata sa CRC u odnosu na kontrolnu grupu (P<0,001, P<0,010, P<0,050 i P<0,050, redom). Koncentracije 25(OH)D3 su bile više kod pacijenata sa gradusom I u odnosu na pacijente sa gradusom II (P<0,050). Svi metaboliti vitamina D su pozitivno korelirali sa ukupnim holesterolom kod pacijenata sa CRC. 25(OH)D3 je bio značajan prediktor povećanog rizika za nastanak CRC (P<0,010). Nakon korekcije za koncentraciju ukupnog holesterola, 25(OH)D3 je izgubio prediktivni značaj. Međutim, 25(OH)D3 je ostao značajan prediktor slabo diferenciranog karcinoma (P<0,050). Zaključak: Utvrđena je značajna pozitivna korelacija iz među statusa vitamina D i serumskih koncentracija ukupnog holesterola. Iako je uočeno da je nizak 25(OH)D3 značajan faktor rizika za razvoj CRC, naši rezultati upućuju na dominantan uticaj holesterola na status vitamina D kod ovih pacijenata. Ipak, uočeno je da niski nivoi 25(OH)D3 mogu nezavisno doprineti razvoju slabo diferentovanih oblika CRC.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer, Povezanost metabolita holesterola i vitamina D sa rizikom za nastanak kolorektalnog karcinoma visokog gradusa",
volume = "39",
number = "3",
pages = "318-327",
doi = "10.2478/jomb-2019-0047"
}

Vladimirov, S., Zeljković, A., Gojković, T., Miljković, M., Stefanović, A., Zeljković, D., Trifunović, B.,& Spasojević-Kalimanovska, V.. (2020). Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(3), 318-327.
https://doi.org/10.2478/jomb-2019-0047

Vladimirov S, Zeljković A, Gojković T, Miljković M, Stefanović A, Zeljković D, Trifunović B, Spasojević-Kalimanovska V. Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer. in Journal of Medical Biochemistry. 2020;39(3):318-327.
doi:10.2478/jomb-2019-0047 .

Vladimirov, Sandra, Zeljković, Aleksandra, Gojković, Tamara, Miljković, Milica, Stefanović, Aleksandra, Zeljković, Dejan, Trifunović, Bratislav, Spasojević-Kalimanovska, Vesna, "Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer" in Journal of Medical Biochemistry, 39, no. 3 (2020):318-327,
https://doi.org/10.2478/jomb-2019-0047 . .

TY  - JOUR
AU  - Mihajlović, Marija
AU  - Gojković, Tamara
AU  - Vladimirov, Sandra
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Vekić, Jelena
AU  - Zeljković, Dejan
AU  - Trifunović, Bratislav
AU  - Kotur-Stevuljević, Jelena
AU  - Spasojević-Kalimanovska, Vesna
AU  - Zeljković, Aleksandra
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3334
AB  - Background: Previous studies revealed decreased level of high-density lipoprotein cholesterol (HDL-C) as important factor for development of colorectal cancer (CRC). Quantity and structure of HDL particles depend on activities of lipid transfer proteins lecithin:cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP), but this topic is largely unexplored in CRC. The main objective of this study was to investigate activities of LCAT and CETP in patients with CRC. Additionally, we analyzed activity of paraoxonase-1 (PON-1), as a main carrier of HDL-antioxidant function. Materials and methods: Ninety-nine CRC patients and 101 healthy individuals were included. LCAT and CETP activities were assessed by measuring rates of formation and transfer of cholesteryl esters. PON-1 paraoxonase and arylesterase activities were measured. Results: Lower levels of HDL-C (p  lt  .001) were observed in cohort of patients, alongside with decreased LCAT (p  lt  .050) and increased CETP activity (p  lt  .050). Both PON-1 activities were diminished in CRC (p  lt  .050 and p  lt  .001 respectively). Univariate logistic regression singled out HDL-C level (OR = 0.218, p  lt  .001), CETP activity (OR = 1.010, p  lt  .01) and mass (OR = 0.994, p  lt  .001) as possible markers of elevated CRC risk. CETP mass maintained its predictive significance when adjusted for traditional risk factors and level of oxidative stress (OR = 0.993, p  lt  .001; OR = 0.982, p  lt  .050, respectively). Conclusion: Our results demonstrated increased CETP and decreased LCAT and PON-1 activities in CRC patients. In preliminary analysis CETP mass was identified as potential significant predictor of CRC development, suggesting that alterations in HDL-C levels, alongside with changes in HDL structure might have a role in carcinogenesis.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Clinical Biochemistry
T1  - Changes in lecithin: cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer
VL  - 63
SP  - 32
EP  - 38
DO  - 10.1016/j.clinbiochem.2018.11.010
ER  - 

@article{
author = "Mihajlović, Marija and Gojković, Tamara and Vladimirov, Sandra and Miljković, Milica and Stefanović, Aleksandra and Vekić, Jelena and Zeljković, Dejan and Trifunović, Bratislav and Kotur-Stevuljević, Jelena and Spasojević-Kalimanovska, Vesna and Zeljković, Aleksandra",
year = "2019",
abstract = "Background: Previous studies revealed decreased level of high-density lipoprotein cholesterol (HDL-C) as important factor for development of colorectal cancer (CRC). Quantity and structure of HDL particles depend on activities of lipid transfer proteins lecithin:cholesterol acyltransferase (LCAT) and cholesteryl ester transfer protein (CETP), but this topic is largely unexplored in CRC. The main objective of this study was to investigate activities of LCAT and CETP in patients with CRC. Additionally, we analyzed activity of paraoxonase-1 (PON-1), as a main carrier of HDL-antioxidant function. Materials and methods: Ninety-nine CRC patients and 101 healthy individuals were included. LCAT and CETP activities were assessed by measuring rates of formation and transfer of cholesteryl esters. PON-1 paraoxonase and arylesterase activities were measured. Results: Lower levels of HDL-C (p  lt  .001) were observed in cohort of patients, alongside with decreased LCAT (p  lt  .050) and increased CETP activity (p  lt  .050). Both PON-1 activities were diminished in CRC (p  lt  .050 and p  lt  .001 respectively). Univariate logistic regression singled out HDL-C level (OR = 0.218, p  lt  .001), CETP activity (OR = 1.010, p  lt  .01) and mass (OR = 0.994, p  lt  .001) as possible markers of elevated CRC risk. CETP mass maintained its predictive significance when adjusted for traditional risk factors and level of oxidative stress (OR = 0.993, p  lt  .001; OR = 0.982, p  lt  .050, respectively). Conclusion: Our results demonstrated increased CETP and decreased LCAT and PON-1 activities in CRC patients. In preliminary analysis CETP mass was identified as potential significant predictor of CRC development, suggesting that alterations in HDL-C levels, alongside with changes in HDL structure might have a role in carcinogenesis.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Clinical Biochemistry",
title = "Changes in lecithin: cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer",
volume = "63",
pages = "32-38",
doi = "10.1016/j.clinbiochem.2018.11.010"
}

Mihajlović, M., Gojković, T., Vladimirov, S., Miljković, M., Stefanović, A., Vekić, J., Zeljković, D., Trifunović, B., Kotur-Stevuljević, J., Spasojević-Kalimanovska, V.,& Zeljković, A.. (2019). Changes in lecithin: cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer. in Clinical Biochemistry
Pergamon-Elsevier Science Ltd, Oxford., 63, 32-38.
https://doi.org/10.1016/j.clinbiochem.2018.11.010

Mihajlović M, Gojković T, Vladimirov S, Miljković M, Stefanović A, Vekić J, Zeljković D, Trifunović B, Kotur-Stevuljević J, Spasojević-Kalimanovska V, Zeljković A. Changes in lecithin: cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer. in Clinical Biochemistry. 2019;63:32-38.
doi:10.1016/j.clinbiochem.2018.11.010 .

Mihajlović, Marija, Gojković, Tamara, Vladimirov, Sandra, Miljković, Milica, Stefanović, Aleksandra, Vekić, Jelena, Zeljković, Dejan, Trifunović, Bratislav, Kotur-Stevuljević, Jelena, Spasojević-Kalimanovska, Vesna, Zeljković, Aleksandra, "Changes in lecithin: cholesterol acyltransferase, cholesteryl ester transfer protein and paraoxonase-1 activities in patients with colorectal cancer" in Clinical Biochemistry, 63 (2019):32-38,
https://doi.org/10.1016/j.clinbiochem.2018.11.010 . .

TY  - JOUR
AU  - Mihajlović, Marija
AU  - Ninić, Ana
AU  - Sopić, Miron
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Vekić, Jelena
AU  - Spasojević-Kalimanovska, Vesna
AU  - Zeljković, Dejan
AU  - Trifunović, Bratislav
AU  - Stjepanović, Željka
AU  - Zeljković, Aleksandra
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3458
AB  - Background: Elevated concentrations of resistin have been reported in colorectal cancer (CRC), but its interactions with adenylate cyclase-associated protein 1 (CAP-1) are largely unexplored. We investigated resistin plasma concentration, peripheral blood mononuclear cells (PBMCs) resistin messenger ribonucleic acid (mRNA), and CAP-1 mRNA levels in CRC patients, as well as the impact of resistin gene polymorphism rs1862513 on the examined markers. We also explored associations of resistin with high-density lipoprotein cholesterol (HDL-C) and predictive potential of our parameters for CRC. Methods: Eighty-six patients with CRC and 75 healthy adults were included. Commercial ELISA kit was used for obtaining resistin’s concentrations, while polymerase chain reaction (PCR) method was applied for evaluation of resistin and CAP-1 mRNA levels and rs1862513 polymorphism. Results: Plasma resistin and CAP-1 mRNA levels were higher in CRC patients (p < 0.001 and p < 0.05, respectively), while resistin mRNA levels were lower (p < 0.001). Negative association existed among plasma resistin and HDL-C concentrations (ρ = − 0.280; p < 0.05). A model including age, body-mass index, HDL-C, low-density lipoprotein cholesterol (LDL-C), and plasma resistin concentrations as independent predictors of CRC showed very good diagnostic accuracy (AUC = 0.898). We found no associations of rs1862513 with the examined markers. Conclusions: Our study demonstrated increased plasma resistin and CAP-1 mRNA levels, implying their possible interaction in CRC. The association among plasma resistin and HDL-C might indicate that HDL-C is involved in alterations of resistin’s secretion process. As a hallmark of personalized medicine, multi-marker approach in determination of resistin-related parameters might be useful for prediction and prevention of CRC development.
PB  - Springer
T2  - EPMA Journal
T1  - Association among resistin, adenylate cyclase-associated protein 1 and high-density lipoprotein cholesterol in patients with colorectal cancer: a multi-marker approach, as a hallmark of innovative predictive, preventive, and personalized medicine
VL  - 10
SP  - 307
EP  - 316
DO  - 10.1007/s13167-019-00178-x
ER  - 

@article{
author = "Mihajlović, Marija and Ninić, Ana and Sopić, Miron and Miljković, Milica and Stefanović, Aleksandra and Vekić, Jelena and Spasojević-Kalimanovska, Vesna and Zeljković, Dejan and Trifunović, Bratislav and Stjepanović, Željka and Zeljković, Aleksandra",
year = "2019",
abstract = "Background: Elevated concentrations of resistin have been reported in colorectal cancer (CRC), but its interactions with adenylate cyclase-associated protein 1 (CAP-1) are largely unexplored. We investigated resistin plasma concentration, peripheral blood mononuclear cells (PBMCs) resistin messenger ribonucleic acid (mRNA), and CAP-1 mRNA levels in CRC patients, as well as the impact of resistin gene polymorphism rs1862513 on the examined markers. We also explored associations of resistin with high-density lipoprotein cholesterol (HDL-C) and predictive potential of our parameters for CRC. Methods: Eighty-six patients with CRC and 75 healthy adults were included. Commercial ELISA kit was used for obtaining resistin’s concentrations, while polymerase chain reaction (PCR) method was applied for evaluation of resistin and CAP-1 mRNA levels and rs1862513 polymorphism. Results: Plasma resistin and CAP-1 mRNA levels were higher in CRC patients (p < 0.001 and p < 0.05, respectively), while resistin mRNA levels were lower (p < 0.001). Negative association existed among plasma resistin and HDL-C concentrations (ρ = − 0.280; p < 0.05). A model including age, body-mass index, HDL-C, low-density lipoprotein cholesterol (LDL-C), and plasma resistin concentrations as independent predictors of CRC showed very good diagnostic accuracy (AUC = 0.898). We found no associations of rs1862513 with the examined markers. Conclusions: Our study demonstrated increased plasma resistin and CAP-1 mRNA levels, implying their possible interaction in CRC. The association among plasma resistin and HDL-C might indicate that HDL-C is involved in alterations of resistin’s secretion process. As a hallmark of personalized medicine, multi-marker approach in determination of resistin-related parameters might be useful for prediction and prevention of CRC development.",
publisher = "Springer",
journal = "EPMA Journal",
title = "Association among resistin, adenylate cyclase-associated protein 1 and high-density lipoprotein cholesterol in patients with colorectal cancer: a multi-marker approach, as a hallmark of innovative predictive, preventive, and personalized medicine",
volume = "10",
pages = "307-316",
doi = "10.1007/s13167-019-00178-x"
}

Mihajlović, M., Ninić, A., Sopić, M., Miljković, M., Stefanović, A., Vekić, J., Spasojević-Kalimanovska, V., Zeljković, D., Trifunović, B., Stjepanović, Ž.,& Zeljković, A.. (2019). Association among resistin, adenylate cyclase-associated protein 1 and high-density lipoprotein cholesterol in patients with colorectal cancer: a multi-marker approach, as a hallmark of innovative predictive, preventive, and personalized medicine. in EPMA Journal
Springer., 10, 307-316.
https://doi.org/10.1007/s13167-019-00178-x

Mihajlović M, Ninić A, Sopić M, Miljković M, Stefanović A, Vekić J, Spasojević-Kalimanovska V, Zeljković D, Trifunović B, Stjepanović Ž, Zeljković A. Association among resistin, adenylate cyclase-associated protein 1 and high-density lipoprotein cholesterol in patients with colorectal cancer: a multi-marker approach, as a hallmark of innovative predictive, preventive, and personalized medicine. in EPMA Journal. 2019;10:307-316.
doi:10.1007/s13167-019-00178-x .

Mihajlović, Marija, Ninić, Ana, Sopić, Miron, Miljković, Milica, Stefanović, Aleksandra, Vekić, Jelena, Spasojević-Kalimanovska, Vesna, Zeljković, Dejan, Trifunović, Bratislav, Stjepanović, Željka, Zeljković, Aleksandra, "Association among resistin, adenylate cyclase-associated protein 1 and high-density lipoprotein cholesterol in patients with colorectal cancer: a multi-marker approach, as a hallmark of innovative predictive, preventive, and personalized medicine" in EPMA Journal, 10 (2019):307-316,
https://doi.org/10.1007/s13167-019-00178-x . .

TY  - JOUR
AU  - Stevanović, Milica
AU  - Vekić, Jelena
AU  - Bogavac-Stanojević, Nataša
AU  - Janać, Jelena
AU  - Stjepanović, Željka
AU  - Zeljković, Dejan
AU  - Trifunović, Bratislav
AU  - Spasojević-Kalimanovska, Vesna
AU  - Zeljković, Aleksandra
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3048
AB  - Introduction: Dyslipidaemia contributes to the occurrence of colorectal cancer (CRC). We hypothesized that qualitative changes of lipoproteins are associated with the risk for CRC development. This study analyses low-density lipoprotein (LDL) and high-density lipoprotein (HDL) diameters, as well as distribution of LDL and HDL subclasses in patients with CRC, with an aim to determine whether advanced lipid testing might be useful in predicting the risk for the onset of this malignancy. Materials and methods: This case-control study included 84 patients with newly diagnosed CRC and 92 controls. Gradient gel electrophoresis was applied for separation of lipoprotein subclasses and for LDL and HDL diameters determination. Lipid parameters were measured using routine enzymatic methods. Results: Total cholesterol, HDL and LDL-cholesterol were significantly lower in CRC patients compared to controls (4.47 mmol/L vs. 5.63 mmol/L; 0.99 mmol/L vs. 1.27 mmol/L; 2.90 mmol/L vs. 3.66 mmol/L; P  lt  0.001, respectively). Patients had significantly smaller LDL (25.14 nm vs. 26.92 nm; P  lt  0.001) and HDL diameters (8.76 nm vs. 10.17 nm; P  lt  0.001) and greater proportion of small, dense LDL particles (54.0% vs. 52.9%; P = 0.044) than controls. Decreased LDL and HDL diameters were independent predictors of CRC (OR = 0.5, P = 0.001 and OR = 0.5, P = 0.008, respectively), and alongside with age and HDL-cholesterol concentrations formed the optimal cost-effective model, providing adequate discriminative abilities for CRC (AUC = 0.89) and correct patients classification (81%). Conclusions: Patients with CRC have decreased LDL and HDL diameters and increased proportion of smaller particles. LDL and HDL diameters determination could be useful in assessing the risk for CRC development.
PB  - Croatian Soc Medical Biochemists, Zagreb
T2  - Biochemia Medica
T1  - Significance of LDL and HDL subclasses characterization in the assessment of risk for colorectal cancer development
VL  - 28
IS  - 3
DO  - 10.11613/BM.2018.030713
ER  - 

@article{
author = "Stevanović, Milica and Vekić, Jelena and Bogavac-Stanojević, Nataša and Janać, Jelena and Stjepanović, Željka and Zeljković, Dejan and Trifunović, Bratislav and Spasojević-Kalimanovska, Vesna and Zeljković, Aleksandra",
year = "2018",
abstract = "Introduction: Dyslipidaemia contributes to the occurrence of colorectal cancer (CRC). We hypothesized that qualitative changes of lipoproteins are associated with the risk for CRC development. This study analyses low-density lipoprotein (LDL) and high-density lipoprotein (HDL) diameters, as well as distribution of LDL and HDL subclasses in patients with CRC, with an aim to determine whether advanced lipid testing might be useful in predicting the risk for the onset of this malignancy. Materials and methods: This case-control study included 84 patients with newly diagnosed CRC and 92 controls. Gradient gel electrophoresis was applied for separation of lipoprotein subclasses and for LDL and HDL diameters determination. Lipid parameters were measured using routine enzymatic methods. Results: Total cholesterol, HDL and LDL-cholesterol were significantly lower in CRC patients compared to controls (4.47 mmol/L vs. 5.63 mmol/L; 0.99 mmol/L vs. 1.27 mmol/L; 2.90 mmol/L vs. 3.66 mmol/L; P  lt  0.001, respectively). Patients had significantly smaller LDL (25.14 nm vs. 26.92 nm; P  lt  0.001) and HDL diameters (8.76 nm vs. 10.17 nm; P  lt  0.001) and greater proportion of small, dense LDL particles (54.0% vs. 52.9%; P = 0.044) than controls. Decreased LDL and HDL diameters were independent predictors of CRC (OR = 0.5, P = 0.001 and OR = 0.5, P = 0.008, respectively), and alongside with age and HDL-cholesterol concentrations formed the optimal cost-effective model, providing adequate discriminative abilities for CRC (AUC = 0.89) and correct patients classification (81%). Conclusions: Patients with CRC have decreased LDL and HDL diameters and increased proportion of smaller particles. LDL and HDL diameters determination could be useful in assessing the risk for CRC development.",
publisher = "Croatian Soc Medical Biochemists, Zagreb",
journal = "Biochemia Medica",
title = "Significance of LDL and HDL subclasses characterization in the assessment of risk for colorectal cancer development",
volume = "28",
number = "3",
doi = "10.11613/BM.2018.030713"
}

Stevanović, M., Vekić, J., Bogavac-Stanojević, N., Janać, J., Stjepanović, Ž., Zeljković, D., Trifunović, B., Spasojević-Kalimanovska, V.,& Zeljković, A.. (2018). Significance of LDL and HDL subclasses characterization in the assessment of risk for colorectal cancer development. in Biochemia Medica
Croatian Soc Medical Biochemists, Zagreb., 28(3).
https://doi.org/10.11613/BM.2018.030713

Stevanović M, Vekić J, Bogavac-Stanojević N, Janać J, Stjepanović Ž, Zeljković D, Trifunović B, Spasojević-Kalimanovska V, Zeljković A. Significance of LDL and HDL subclasses characterization in the assessment of risk for colorectal cancer development. in Biochemia Medica. 2018;28(3).
doi:10.11613/BM.2018.030713 .

Stevanović, Milica, Vekić, Jelena, Bogavac-Stanojević, Nataša, Janać, Jelena, Stjepanović, Željka, Zeljković, Dejan, Trifunović, Bratislav, Spasojević-Kalimanovska, Vesna, Zeljković, Aleksandra, "Significance of LDL and HDL subclasses characterization in the assessment of risk for colorectal cancer development" in Biochemia Medica, 28, no. 3 (2018),
https://doi.org/10.11613/BM.2018.030713 . .