TY  - JOUR
AU  - Antonić, Tamara
AU  - Ardalić, Daniela
AU  - Vladimirov, Sandra
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Mitrović, Marija
AU  - Ivanišević, Jasmina
AU  - Gojković, Tamara
AU  - Munjas, Jelena
AU  - Spasojević-Kalimanovska, Vesna
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4957
AB  - A specific feature of dyslipidemia in pregnancy is increased high-density lipoprotein (HDL) cholesterol concentration, which is probably associated with maternal endothelium protection. However, preeclampsia is most often associated with low HDL cholesterol, and the mechanisms behind this change are scarcely explored. We aimed to investigate changes in HDL metabolism in risky pregnancies and those complicated by late-onset preeclampsia. We analyze cholesterol synthesis (cholesterol precursors: desmosterol, 7-dehydrocholesterol, and lathosterol) and absorption markers (phytosterols: campesterol and β-sitosterol) within HDL particles (NCSHDL), the activities of principal modulators of HDL cholesterol’s content, and major HDL functional proteins levels in mid and late pregnancy. On the basis of the pregnancy outcome, participants were classified into the risk group (RG) (70 women) and the preeclampsia group (PG) (20 women). HDL cholesterol was lower in PG in the second trimester compared to RG (p < 0.05) and followed by lower levels of cholesterol absorption markers (p < 0.001 for campesterolHDL and p < 0.05 for β-sitosterolHDL). Lowering of HDL cholesterol between trimesters in RG (p < 0.05) was accompanied by a decrease in HDL phytosterol content (p < 0.001), apolipoprotein A-I (apoA-I) concentration (p < 0.05), and paraoxonase 1 (PON1) (p < 0.001), lecithin–cholesterol acyltransferase (LCAT) (p < 0.05), and cholesterol ester transfer protein (CETP) activities (p < 0.05). These longitudinal changes were absent in PG. Development of late-onset preeclampsia is preceded by the appearance of lower HDL cholesterol and NCSHDL in the second trimester. We propose that reduced capacity for intestinal HDL synthesis, decreased LCAT activity, and impaired capacity for HDL-mediated cholesterol efflux could be the contributing mechanisms resulting in lower HDL cholesterol.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Cholesterol Metabolic Profiling of HDL in Women with Late-Onset Preeclampsia
VL  - 24
IS  - 14
DO  - 10.3390/ijms241411357
ER  - 

@article{
author = "Antonić, Tamara and Ardalić, Daniela and Vladimirov, Sandra and Zeljković, Aleksandra and Vekić, Jelena and Mitrović, Marija and Ivanišević, Jasmina and Gojković, Tamara and Munjas, Jelena and Spasojević-Kalimanovska, Vesna and Miković, Željko and Stefanović, Aleksandra",
year = "2023",
abstract = "A specific feature of dyslipidemia in pregnancy is increased high-density lipoprotein (HDL) cholesterol concentration, which is probably associated with maternal endothelium protection. However, preeclampsia is most often associated with low HDL cholesterol, and the mechanisms behind this change are scarcely explored. We aimed to investigate changes in HDL metabolism in risky pregnancies and those complicated by late-onset preeclampsia. We analyze cholesterol synthesis (cholesterol precursors: desmosterol, 7-dehydrocholesterol, and lathosterol) and absorption markers (phytosterols: campesterol and β-sitosterol) within HDL particles (NCSHDL), the activities of principal modulators of HDL cholesterol’s content, and major HDL functional proteins levels in mid and late pregnancy. On the basis of the pregnancy outcome, participants were classified into the risk group (RG) (70 women) and the preeclampsia group (PG) (20 women). HDL cholesterol was lower in PG in the second trimester compared to RG (p < 0.05) and followed by lower levels of cholesterol absorption markers (p < 0.001 for campesterolHDL and p < 0.05 for β-sitosterolHDL). Lowering of HDL cholesterol between trimesters in RG (p < 0.05) was accompanied by a decrease in HDL phytosterol content (p < 0.001), apolipoprotein A-I (apoA-I) concentration (p < 0.05), and paraoxonase 1 (PON1) (p < 0.001), lecithin–cholesterol acyltransferase (LCAT) (p < 0.05), and cholesterol ester transfer protein (CETP) activities (p < 0.05). These longitudinal changes were absent in PG. Development of late-onset preeclampsia is preceded by the appearance of lower HDL cholesterol and NCSHDL in the second trimester. We propose that reduced capacity for intestinal HDL synthesis, decreased LCAT activity, and impaired capacity for HDL-mediated cholesterol efflux could be the contributing mechanisms resulting in lower HDL cholesterol.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Cholesterol Metabolic Profiling of HDL in Women with Late-Onset Preeclampsia",
volume = "24",
number = "14",
doi = "10.3390/ijms241411357"
}

Antonić, T., Ardalić, D., Vladimirov, S., Zeljković, A., Vekić, J., Mitrović, M., Ivanišević, J., Gojković, T., Munjas, J., Spasojević-Kalimanovska, V., Miković, Ž.,& Stefanović, A.. (2023). Cholesterol Metabolic Profiling of HDL in Women with Late-Onset Preeclampsia. in International Journal of Molecular Sciences
MDPI., 24(14).
https://doi.org/10.3390/ijms241411357

Antonić T, Ardalić D, Vladimirov S, Zeljković A, Vekić J, Mitrović M, Ivanišević J, Gojković T, Munjas J, Spasojević-Kalimanovska V, Miković Ž, Stefanović A. Cholesterol Metabolic Profiling of HDL in Women with Late-Onset Preeclampsia. in International Journal of Molecular Sciences. 2023;24(14).
doi:10.3390/ijms241411357 .

Antonić, Tamara, Ardalić, Daniela, Vladimirov, Sandra, Zeljković, Aleksandra, Vekić, Jelena, Mitrović, Marija, Ivanišević, Jasmina, Gojković, Tamara, Munjas, Jelena, Spasojević-Kalimanovska, Vesna, Miković, Željko, Stefanović, Aleksandra, "Cholesterol Metabolic Profiling of HDL in Women with Late-Onset Preeclampsia" in International Journal of Molecular Sciences, 24, no. 14 (2023),
https://doi.org/10.3390/ijms241411357 . .

TY  - JOUR
AU  - Ivanišević, Jasmina
AU  - Ardalić, Daniela
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Gojković, Tamara
AU  - Vladimirov, Sandra
AU  - Antonić, Tamara
AU  - Munjas, Jelena
AU  - Stefanović, Aleksandra
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4637
AB  - The 1st trimester of pregnancy is accompanied with changes in different biochemical and
hematological parameters. Analyses scheduled to be performed in the 1st trimester are complete
blood count, blood group, Rh factor and the double test. Many experts also suggest the
determination of lipid status parameters as a routine analysis in the early pregnancy. Reliable data
about maternal and fetal health can be obtained by the assessment of the above-mentioned
parameters. They may be helpful in assessing the risk for pregnancy complication development
and/or perinatal adverse outcomes.
AB  - Prvi trimestar trudnoće praćen je promenama različitih biohemijskih i hematoloških
parametara. Analize koje se rade u 1. trimestru su kompletna krvna slika, krvna grupa, Rh faktor
i tzv. „double“ test. Mnogi stručnjaci predlažu određivanje parametara lipidnog statusa kao
rutinsku analizu u ranoj trudnoći. Procenom gore navedenih parametara mogu se dobiti pouzdani
podaci o zdravlju majke i fetusa, a mogu da posluže i za procenu rizika za razvoj komplikacija u
trudnoći i/ili perinatalnih neželjenih ishoda.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
T2  - Arhiv za farmaciju
T1  - Biochemical and hematological parameters in the  1 st  trimester of pregnancy
T1  - Biohemijski i hematološki parametri u prvom  trimestru trudnoće
VL  - 73
IS  - 1
SP  - 62
EP  - 73
DO  - 10.5937/arhfarm73-41999
ER  - 

@article{
author = "Ivanišević, Jasmina and Ardalić, Daniela and Zeljković, Aleksandra and Vekić, Jelena and Gojković, Tamara and Vladimirov, Sandra and Antonić, Tamara and Munjas, Jelena and Stefanović, Aleksandra",
year = "2023",
abstract = "The 1st trimester of pregnancy is accompanied with changes in different biochemical and
hematological parameters. Analyses scheduled to be performed in the 1st trimester are complete
blood count, blood group, Rh factor and the double test. Many experts also suggest the
determination of lipid status parameters as a routine analysis in the early pregnancy. Reliable data
about maternal and fetal health can be obtained by the assessment of the above-mentioned
parameters. They may be helpful in assessing the risk for pregnancy complication development
and/or perinatal adverse outcomes., Prvi trimestar trudnoće praćen je promenama različitih biohemijskih i hematoloških
parametara. Analize koje se rade u 1. trimestru su kompletna krvna slika, krvna grupa, Rh faktor
i tzv. „double“ test. Mnogi stručnjaci predlažu određivanje parametara lipidnog statusa kao
rutinsku analizu u ranoj trudnoći. Procenom gore navedenih parametara mogu se dobiti pouzdani
podaci o zdravlju majke i fetusa, a mogu da posluže i za procenu rizika za razvoj komplikacija u
trudnoći i/ili perinatalnih neželjenih ishoda.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Biochemical and hematological parameters in the  1 st  trimester of pregnancy, Biohemijski i hematološki parametri u prvom  trimestru trudnoće",
volume = "73",
number = "1",
pages = "62-73",
doi = "10.5937/arhfarm73-41999"
}

Ivanišević, J., Ardalić, D., Zeljković, A., Vekić, J., Gojković, T., Vladimirov, S., Antonić, T., Munjas, J.,& Stefanović, A.. (2023). Biochemical and hematological parameters in the  1 st  trimester of pregnancy. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 73(1), 62-73.
https://doi.org/10.5937/arhfarm73-41999

Ivanišević J, Ardalić D, Zeljković A, Vekić J, Gojković T, Vladimirov S, Antonić T, Munjas J, Stefanović A. Biochemical and hematological parameters in the  1 st  trimester of pregnancy. in Arhiv za farmaciju. 2023;73(1):62-73.
doi:10.5937/arhfarm73-41999 .

Ivanišević, Jasmina, Ardalić, Daniela, Zeljković, Aleksandra, Vekić, Jelena, Gojković, Tamara, Vladimirov, Sandra, Antonić, Tamara, Munjas, Jelena, Stefanović, Aleksandra, "Biochemical and hematological parameters in the  1 st  trimester of pregnancy" in Arhiv za farmaciju, 73, no. 1 (2023):62-73,
https://doi.org/10.5937/arhfarm73-41999 . .

TY  - JOUR
AU  - Banjac, Gorica
AU  - Ardalić, Daniela
AU  - Cabunac, Petar
AU  - Ivanišević, Jasmina
AU  - Vekić, Jelena
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4659
AB  - Background/Aim. One of the complications that can oc-
cur during pregnancy is the development of preeclampsia
(PE). The main characteristics of this condition are high
blood pressure and very often signs of kidney damage or
other organ damage. The condition affects 5–7% of all
pregnant women and is one of the main factors of maternal
and perinatal morbidity and mortality worldwide. The aim
of this study was to investigate the structural and functional
modifications of high-density lipoprotein (HDL) particles
during high-risk pregnancies (HRP) for PE development.
Methods. The longitudinal prospective study included a to-
tal of 91 pregnant women with a HRP for developing PE.
Out of this total number, 71 women did not develop PE
until delivery, and this group was designated as the group
without PE (WPE). The rest of the 20 HRP women devel-
oped PE before delivery and were designated as the PE
group. The blood was sampled toward the end of each tri-
mester and before the delivery. The distribution of HDL
particles was determined by the vertical 3–31% polyacryla-
mide gradient gel electrophoresis method. The antioxidative capacity of HDL particles was measured by the activity of
the HDL-associated enzyme – paraoxonase 1 (PON1).
PON1 activity was determined by the method of kinetic
spectrophotometry from serum samples. Results. The re-
sults have shown that the proportions of HDL 2b particles
significantly increased in the 2nd trimester (p ˂ 0.05) and
remained increased until the end of pregnancy in the WPE
group. PON1 activity was significantly higher in the 3 rd tri-
mester (p < 0.05) of the WPE group. In the PE group, we
found that the proportions of HDL 3a particles significantly
decreased in the 2 nd trimester (p < 0.05) and remained de-
creased until the end of pregnancy. PON1 activity has not
changed in the PE group during pregnancy. Conclusion.
Dyslipidemia in pregnancy could be associated with differ-
ent modifications of HDL particles. The adaptive pregnancy
mechanisms expressed as a functional modification of HDL
particles in pregnant women who develop PE seem inade-
quate and, therefore, lose their atheroprotective role.
AB  - Uvod/Cilj. Jedna od komplikacija koja se može javiti
tokom trudnoće je razvoj preeklampsije (PE). Glavne
karakteristike ovog stanja su visok krvni pritisak i vrlo često
znaci oštećenja bubrega ili drugih organa. Ovo stanje
pogađa 5–7% svih trudnica i jedan je od glavnih faktora
morbiditeta i mortaliteta trudnica i fetusa ili novorođenčadi.
Cilj ovog rada bio je ispitivanje strukturnih i funkcionalnih
modifikacija lipoproteinskih čestica visoke gustine (high-
density lipoprotein – HDL) u trudnoćama sa visokim rizikom
(TVR) za razvoj PE. Metode. U longitudinalnoj
prospektivnoj studiji učestvovala je ukupno 91 trudnica sa
TVR od razvoja PE. Od ukupnog broja trudnica, kod njih 71, PE se nije razvila do kraja trudnoće i ova grupa je
označena kao grupa bez PE (BPE). Kod preostalih 20 žena
sa TVR se razvila PE do porođaja, i one su svrstane u grupu
PE. Krv je uzimana za analizu na kraju svakog trimestra i
pred porođaj. Raspodela HDL čestica je određivana
metodom vertikalne elektroforeze u 3–31% gradijentu
poliakrilamidnog gela. Antioksidativni kapacitet HDL
čestica je određivan na osnovu aktivnosti enzima
paraoksonaze 1 (PON1) vezanog za HDL. Aktivnost
PON1 u serumu je određivana metodom kinetičke
spektrofotometrije. Rezultati. Rezultati su pokazali da se
udeo HDL 2b čestica značajno povećao u drugom trimestru
(p < 0,05) i ostao je povećan do kraja trudnoće u grupi BPE.
Aktivnost PON1 bila je značajno veća u trećem trimestru (p < 0,05) kod ove grupe trudnica. Udeo HDL 3a
čestica se značajno smanjio u drugom trimestru u PE grupi
trudnica (p < 0,05) i ostao je snižen do kraja trudno će.
Aktivnost PON1 enzima u PE gripi se nije menjala u toku
trudnoće. Zaključak. Dislipidemija u trudnoći može biti
posledica različitih modifikacija HDL čestica. Strukturne i
funkcionalne modifikacije HDL čestica, kao jedan od adaptivnih mehanizama, kod trudnica kod kojih se razvila
PE, nisu adekvatne i kao takve gube svoju ateroprotektivnu
ulogu.
PB  - Univerzitet odbrane - Vojnomedicinska akademija, Medicinski fakultet
T2  - Vojnosanitetski pregled
T1  - Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia
T1  - Izmenjena struktura i antioksidativni kapacitet lipoproteinskih čestica visoke gustine u preeklampsiji
VL  - 80
IS  - 2
SP  - 121
EP  - 127
DO  - 10.2298/VSP210413010B
ER  - 

@article{
author = "Banjac, Gorica and Ardalić, Daniela and Cabunac, Petar and Ivanišević, Jasmina and Vekić, Jelena and Miković, Željko and Stefanović, Aleksandra",
year = "2023",
abstract = "Background/Aim. One of the complications that can oc-
cur during pregnancy is the development of preeclampsia
(PE). The main characteristics of this condition are high
blood pressure and very often signs of kidney damage or
other organ damage. The condition affects 5–7% of all
pregnant women and is one of the main factors of maternal
and perinatal morbidity and mortality worldwide. The aim
of this study was to investigate the structural and functional
modifications of high-density lipoprotein (HDL) particles
during high-risk pregnancies (HRP) for PE development.
Methods. The longitudinal prospective study included a to-
tal of 91 pregnant women with a HRP for developing PE.
Out of this total number, 71 women did not develop PE
until delivery, and this group was designated as the group
without PE (WPE). The rest of the 20 HRP women devel-
oped PE before delivery and were designated as the PE
group. The blood was sampled toward the end of each tri-
mester and before the delivery. The distribution of HDL
particles was determined by the vertical 3–31% polyacryla-
mide gradient gel electrophoresis method. The antioxidative capacity of HDL particles was measured by the activity of
the HDL-associated enzyme – paraoxonase 1 (PON1).
PON1 activity was determined by the method of kinetic
spectrophotometry from serum samples. Results. The re-
sults have shown that the proportions of HDL 2b particles
significantly increased in the 2nd trimester (p ˂ 0.05) and
remained increased until the end of pregnancy in the WPE
group. PON1 activity was significantly higher in the 3 rd tri-
mester (p < 0.05) of the WPE group. In the PE group, we
found that the proportions of HDL 3a particles significantly
decreased in the 2 nd trimester (p < 0.05) and remained de-
creased until the end of pregnancy. PON1 activity has not
changed in the PE group during pregnancy. Conclusion.
Dyslipidemia in pregnancy could be associated with differ-
ent modifications of HDL particles. The adaptive pregnancy
mechanisms expressed as a functional modification of HDL
particles in pregnant women who develop PE seem inade-
quate and, therefore, lose their atheroprotective role., Uvod/Cilj. Jedna od komplikacija koja se može javiti
tokom trudnoće je razvoj preeklampsije (PE). Glavne
karakteristike ovog stanja su visok krvni pritisak i vrlo često
znaci oštećenja bubrega ili drugih organa. Ovo stanje
pogađa 5–7% svih trudnica i jedan je od glavnih faktora
morbiditeta i mortaliteta trudnica i fetusa ili novorođenčadi.
Cilj ovog rada bio je ispitivanje strukturnih i funkcionalnih
modifikacija lipoproteinskih čestica visoke gustine (high-
density lipoprotein – HDL) u trudnoćama sa visokim rizikom
(TVR) za razvoj PE. Metode. U longitudinalnoj
prospektivnoj studiji učestvovala je ukupno 91 trudnica sa
TVR od razvoja PE. Od ukupnog broja trudnica, kod njih 71, PE se nije razvila do kraja trudnoće i ova grupa je
označena kao grupa bez PE (BPE). Kod preostalih 20 žena
sa TVR se razvila PE do porođaja, i one su svrstane u grupu
PE. Krv je uzimana za analizu na kraju svakog trimestra i
pred porođaj. Raspodela HDL čestica je određivana
metodom vertikalne elektroforeze u 3–31% gradijentu
poliakrilamidnog gela. Antioksidativni kapacitet HDL
čestica je određivan na osnovu aktivnosti enzima
paraoksonaze 1 (PON1) vezanog za HDL. Aktivnost
PON1 u serumu je određivana metodom kinetičke
spektrofotometrije. Rezultati. Rezultati su pokazali da se
udeo HDL 2b čestica značajno povećao u drugom trimestru
(p < 0,05) i ostao je povećan do kraja trudnoće u grupi BPE.
Aktivnost PON1 bila je značajno veća u trećem trimestru (p < 0,05) kod ove grupe trudnica. Udeo HDL 3a
čestica se značajno smanjio u drugom trimestru u PE grupi
trudnica (p < 0,05) i ostao je snižen do kraja trudno će.
Aktivnost PON1 enzima u PE gripi se nije menjala u toku
trudnoće. Zaključak. Dislipidemija u trudnoći može biti
posledica različitih modifikacija HDL čestica. Strukturne i
funkcionalne modifikacije HDL čestica, kao jedan od adaptivnih mehanizama, kod trudnica kod kojih se razvila
PE, nisu adekvatne i kao takve gube svoju ateroprotektivnu
ulogu.",
publisher = "Univerzitet odbrane - Vojnomedicinska akademija, Medicinski fakultet",
journal = "Vojnosanitetski pregled",
title = "Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia, Izmenjena struktura i antioksidativni kapacitet lipoproteinskih čestica visoke gustine u preeklampsiji",
volume = "80",
number = "2",
pages = "121-127",
doi = "10.2298/VSP210413010B"
}

Banjac, G., Ardalić, D., Cabunac, P., Ivanišević, J., Vekić, J., Miković, Ž.,& Stefanović, A.. (2023). Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia. in Vojnosanitetski pregled
Univerzitet odbrane - Vojnomedicinska akademija, Medicinski fakultet., 80(2), 121-127.
https://doi.org/10.2298/VSP210413010B

Banjac G, Ardalić D, Cabunac P, Ivanišević J, Vekić J, Miković Ž, Stefanović A. Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia. in Vojnosanitetski pregled. 2023;80(2):121-127.
doi:10.2298/VSP210413010B .

Banjac, Gorica, Ardalić, Daniela, Cabunac, Petar, Ivanišević, Jasmina, Vekić, Jelena, Miković, Željko, Stefanović, Aleksandra, "Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia" in Vojnosanitetski pregled, 80, no. 2 (2023):121-127,
https://doi.org/10.2298/VSP210413010B . .

TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Ardalić, Daniela
AU  - Vekić, Jelena
AU  - Antonić, Tamara
AU  - Vladimirov, Sandra
AU  - Rizzo, Manfredi
AU  - Gojković, Tamara
AU  - Ivanišević, Jasmina
AU  - Mihajlović, Marija
AU  - Vujčić, Sanja
AU  - Cabunac, Petar
AU  - Spasojević-Kalimanovska, Vesna
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4314
AB  - Metabolic disorders in pregnancy, particularly gestational diabetes mellitus (GDM), are associated with an increased risk for adverse pregnancy outcome and long-term cardiometabolic health of mother and child. This study analyzed changes of serum cholesterol synthesis and absorption markers during the course of high-risk pregnancies, with respect to the development of GDM. Possible associations of maternal lipid biomarkers with neonatal characteristics were also investigated. The study included 63 women with high risk for development of pregnancy complications. Size and proportions of small low-density (LDL) and high-density lipoprotein (HDL) particles were assessed across trimesters (T1–T3), as well as concentrations of cholesterol synthesis (lathosterol, desmosterol) and absorption markers (campesterol, β-sitosterol). During the study, 15 women developed GDM, while 48 had no complications (non-GDM). As compared to the non-GDM group, women with GDM had significantly higher triglycerides in each trimester, while having a lower HDL-C level in T3. In addition, they had significantly lower levels of β-sitosterol in T3 (p < 0.05). Cholesterol synthesis markers increased across trimesters in both groups. A decrease in serum β-sitosterol levels during the course of pregnancies affected by GDM was observed. The prevalence of small-sized HDL decreased in non-GDM, while in the GDM group remained unchanged across trimesters. Newborn’s size in the non-GDM group was significantly higher (p < 0.01) and inversely associated with proportions of both small, dense LDL and HDL particles (p < 0.05) in maternal plasma in T1. In conclusion, high-risk pregnancies affected by GDM are characterized by altered cholesterol absorption and HDL maturation. Advanced lipid testing may indicate disturbed lipid homeostasis in GDM.
PB  - MDPI
T2  - Metabolites
T1  - Effects of Gestational Diabetes Mellitus on Cholesterol Metabolism in Women with High-Risk Pregnancies: Possible Implications for Neonatal Outcome
VL  - 12
IS  - 10
DO  - 10.3390/metabo12100959
ER  - 

@article{
author = "Zeljković, Aleksandra and Ardalić, Daniela and Vekić, Jelena and Antonić, Tamara and Vladimirov, Sandra and Rizzo, Manfredi and Gojković, Tamara and Ivanišević, Jasmina and Mihajlović, Marija and Vujčić, Sanja and Cabunac, Petar and Spasojević-Kalimanovska, Vesna and Miković, Željko and Stefanović, Aleksandra",
year = "2022",
abstract = "Metabolic disorders in pregnancy, particularly gestational diabetes mellitus (GDM), are associated with an increased risk for adverse pregnancy outcome and long-term cardiometabolic health of mother and child. This study analyzed changes of serum cholesterol synthesis and absorption markers during the course of high-risk pregnancies, with respect to the development of GDM. Possible associations of maternal lipid biomarkers with neonatal characteristics were also investigated. The study included 63 women with high risk for development of pregnancy complications. Size and proportions of small low-density (LDL) and high-density lipoprotein (HDL) particles were assessed across trimesters (T1–T3), as well as concentrations of cholesterol synthesis (lathosterol, desmosterol) and absorption markers (campesterol, β-sitosterol). During the study, 15 women developed GDM, while 48 had no complications (non-GDM). As compared to the non-GDM group, women with GDM had significantly higher triglycerides in each trimester, while having a lower HDL-C level in T3. In addition, they had significantly lower levels of β-sitosterol in T3 (p < 0.05). Cholesterol synthesis markers increased across trimesters in both groups. A decrease in serum β-sitosterol levels during the course of pregnancies affected by GDM was observed. The prevalence of small-sized HDL decreased in non-GDM, while in the GDM group remained unchanged across trimesters. Newborn’s size in the non-GDM group was significantly higher (p < 0.01) and inversely associated with proportions of both small, dense LDL and HDL particles (p < 0.05) in maternal plasma in T1. In conclusion, high-risk pregnancies affected by GDM are characterized by altered cholesterol absorption and HDL maturation. Advanced lipid testing may indicate disturbed lipid homeostasis in GDM.",
publisher = "MDPI",
journal = "Metabolites",
title = "Effects of Gestational Diabetes Mellitus on Cholesterol Metabolism in Women with High-Risk Pregnancies: Possible Implications for Neonatal Outcome",
volume = "12",
number = "10",
doi = "10.3390/metabo12100959"
}

Zeljković, A., Ardalić, D., Vekić, J., Antonić, T., Vladimirov, S., Rizzo, M., Gojković, T., Ivanišević, J., Mihajlović, M., Vujčić, S., Cabunac, P., Spasojević-Kalimanovska, V., Miković, Ž.,& Stefanović, A.. (2022). Effects of Gestational Diabetes Mellitus on Cholesterol Metabolism in Women with High-Risk Pregnancies: Possible Implications for Neonatal Outcome. in Metabolites
MDPI., 12(10).
https://doi.org/10.3390/metabo12100959

Zeljković A, Ardalić D, Vekić J, Antonić T, Vladimirov S, Rizzo M, Gojković T, Ivanišević J, Mihajlović M, Vujčić S, Cabunac P, Spasojević-Kalimanovska V, Miković Ž, Stefanović A. Effects of Gestational Diabetes Mellitus on Cholesterol Metabolism in Women with High-Risk Pregnancies: Possible Implications for Neonatal Outcome. in Metabolites. 2022;12(10).
doi:10.3390/metabo12100959 .

Zeljković, Aleksandra, Ardalić, Daniela, Vekić, Jelena, Antonić, Tamara, Vladimirov, Sandra, Rizzo, Manfredi, Gojković, Tamara, Ivanišević, Jasmina, Mihajlović, Marija, Vujčić, Sanja, Cabunac, Petar, Spasojević-Kalimanovska, Vesna, Miković, Željko, Stefanović, Aleksandra, "Effects of Gestational Diabetes Mellitus on Cholesterol Metabolism in Women with High-Risk Pregnancies: Possible Implications for Neonatal Outcome" in Metabolites, 12, no. 10 (2022),
https://doi.org/10.3390/metabo12100959 . .

TY  - CONF
AU  - Antonić, Tamara
AU  - Ardalić, Daniela
AU  - Vladimirov, Sandra
AU  - Marković, Gorica
AU  - Cabunac, Petar
AU  - Mihajlović, Marija
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4644
AB  - Despite significant progress in improving pregnancy outcomes in recent decades,
predicting the risk and treatment of preeclampsia are still major challenges in clinical
practice (1). The aim of this study was to examine non-routine biomarkers in preeclampsia
risk assessment. The study involved 90 women with high-risk pregnancies, 20 of whom
developed preeclampsia by the end of pregnancy. Biochemical parameters were determined
between the 12th and 13 th weeks of gestation. The results of the study showed that women
who later developed preeclampsia had higher concentrations of lathosterol, cholesterol
synthesis marker (p <0.05), inflammatory proteins - monocyte chemoattractant protein-1
(MCP-1), and resistin (p <0.01, both), as well as paraoxonase-1 (PON1) activity (p <0.05).
Binary logistic regression analysis showed that higher concentrations of lathosterol, MCP-1,
resistin, and PON-1 were associated with preeclampsia development. To determine whether
the parameters significant in univariate analysis, are independent predictors of
preeclampsia, we applied multivariate regression analysis. Clinical markers commonly used
in risk assessment (maternal age and body mass index, mean arterial pressure, and uterine
blood flow), lathosterol, MCP-1, resistin, and PON-1 were included in the model. MCP-1 and
resistin stood out as significant independent predictors of preeclampsia. The diagnostic
accuracy of the investigated model was excellent (AUC=0.859). The study results indicated
the importance of a multi-marker approach in risk assessment for preeclampsia
development.
AB  - Uprkos značajnom napretku u poboljšanju ishoda trudnoće poslednjih decenija,
predviđanje rizika i terapija preeklampsije su još uvijek veliki izazovi u kliničkoj praksi (1).
Cilj ove studije je bio ispitivanje biomarkera koji se ne koriste u rutinskoj praksi u proceni
rizika za razvoj preeklampsije. U studiji je učestvovalo 90 žena sa visokorizičnim
trudnoćama, od kojih je 20 razvilo preeklampsiju do kraja trudnoće. Biohemijski parametri
su određivani između 12. i 13. nedelje gestacije. Rezultati studije su pokazali da su žene koje
su razvile preeklampsiju imale više koncentracije latosterola, markera sinteze holesterola (p
<0,05), inflamatornih proteina - monocitnog hemoatraktantnog proteina-1 (MCP-1) i
rezistina (p < 0,01, oba), kao i aktivnost enzima paraoksonaze-1 (PON1) (p <0,05). Binarna
logistička regresiona analiza je pokazala da su više koncentracije latosterola, MCP-1,
rezistina i PON-1 povezane sa razvojem preeklampsije. Da bi se utvrdilo da su parametri koji
su se u univarijantnoj analizi pokazali značajnim, nezavisni prediktori preeklampsije,
primjenili smo multivarijantnu regresionu analizu. U model su ušli klinički parametri koji se
uobičajeno koriste u procjeni rizika (starost i indeks tjelesne mase majke, srednji arterijski
pritisak i protok krvi kroz matericu), latosterol, MCP-1, rezistin i PON-1. MCP-1 i rezistin su
se istakli kao značajni nezavisni prediktori preeklampsije. Pokazana je odlična dijagnostička
tačnost ispitivanog modela (AUC=0,859). Rezultati ove studije su ukazali na značaj
multimarkerskog pristupa u procjeni rizika za razvoj preeklampsije.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Novel biomarkers in preeclampsia risk assessment
T1  - Novi biomarkeri u procjeni rizika za razvoj preeklampsije
VL  - 72
IS  - 4 suplement
SP  - S620
EP  - S621
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4644
ER  - 

@conference{
author = "Antonić, Tamara and Ardalić, Daniela and Vladimirov, Sandra and Marković, Gorica and Cabunac, Petar and Mihajlović, Marija and Miković, Željko and Stefanović, Aleksandra",
year = "2022",
abstract = "Despite significant progress in improving pregnancy outcomes in recent decades,
predicting the risk and treatment of preeclampsia are still major challenges in clinical
practice (1). The aim of this study was to examine non-routine biomarkers in preeclampsia
risk assessment. The study involved 90 women with high-risk pregnancies, 20 of whom
developed preeclampsia by the end of pregnancy. Biochemical parameters were determined
between the 12th and 13 th weeks of gestation. The results of the study showed that women
who later developed preeclampsia had higher concentrations of lathosterol, cholesterol
synthesis marker (p <0.05), inflammatory proteins - monocyte chemoattractant protein-1
(MCP-1), and resistin (p <0.01, both), as well as paraoxonase-1 (PON1) activity (p <0.05).
Binary logistic regression analysis showed that higher concentrations of lathosterol, MCP-1,
resistin, and PON-1 were associated with preeclampsia development. To determine whether
the parameters significant in univariate analysis, are independent predictors of
preeclampsia, we applied multivariate regression analysis. Clinical markers commonly used
in risk assessment (maternal age and body mass index, mean arterial pressure, and uterine
blood flow), lathosterol, MCP-1, resistin, and PON-1 were included in the model. MCP-1 and
resistin stood out as significant independent predictors of preeclampsia. The diagnostic
accuracy of the investigated model was excellent (AUC=0.859). The study results indicated
the importance of a multi-marker approach in risk assessment for preeclampsia
development., Uprkos značajnom napretku u poboljšanju ishoda trudnoće poslednjih decenija,
predviđanje rizika i terapija preeklampsije su još uvijek veliki izazovi u kliničkoj praksi (1).
Cilj ove studije je bio ispitivanje biomarkera koji se ne koriste u rutinskoj praksi u proceni
rizika za razvoj preeklampsije. U studiji je učestvovalo 90 žena sa visokorizičnim
trudnoćama, od kojih je 20 razvilo preeklampsiju do kraja trudnoće. Biohemijski parametri
su određivani između 12. i 13. nedelje gestacije. Rezultati studije su pokazali da su žene koje
su razvile preeklampsiju imale više koncentracije latosterola, markera sinteze holesterola (p
<0,05), inflamatornih proteina - monocitnog hemoatraktantnog proteina-1 (MCP-1) i
rezistina (p < 0,01, oba), kao i aktivnost enzima paraoksonaze-1 (PON1) (p <0,05). Binarna
logistička regresiona analiza je pokazala da su više koncentracije latosterola, MCP-1,
rezistina i PON-1 povezane sa razvojem preeklampsije. Da bi se utvrdilo da su parametri koji
su se u univarijantnoj analizi pokazali značajnim, nezavisni prediktori preeklampsije,
primjenili smo multivarijantnu regresionu analizu. U model su ušli klinički parametri koji se
uobičajeno koriste u procjeni rizika (starost i indeks tjelesne mase majke, srednji arterijski
pritisak i protok krvi kroz matericu), latosterol, MCP-1, rezistin i PON-1. MCP-1 i rezistin su
se istakli kao značajni nezavisni prediktori preeklampsije. Pokazana je odlična dijagnostička
tačnost ispitivanog modela (AUC=0,859). Rezultati ove studije su ukazali na značaj
multimarkerskog pristupa u procjeni rizika za razvoj preeklampsije.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Novel biomarkers in preeclampsia risk assessment, Novi biomarkeri u procjeni rizika za razvoj preeklampsije",
volume = "72",
number = "4 suplement",
pages = "S620-S621",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4644"
}

Antonić, T., Ardalić, D., Vladimirov, S., Marković, G., Cabunac, P., Mihajlović, M., Miković, Ž.,& Stefanović, A.. (2022). Novel biomarkers in preeclampsia risk assessment. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S620-S621.
https://hdl.handle.net/21.15107/rcub_farfar_4644

Antonić T, Ardalić D, Vladimirov S, Marković G, Cabunac P, Mihajlović M, Miković Ž, Stefanović A. Novel biomarkers in preeclampsia risk assessment. in Arhiv za farmaciju. 2022;72(4 suplement):S620-S621.
https://hdl.handle.net/21.15107/rcub_farfar_4644 .

Antonić, Tamara, Ardalić, Daniela, Vladimirov, Sandra, Marković, Gorica, Cabunac, Petar, Mihajlović, Marija, Miković, Željko, Stefanović, Aleksandra, "Novel biomarkers in preeclampsia risk assessment" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S620-S621,
https://hdl.handle.net/21.15107/rcub_farfar_4644 .

TY  - CONF
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Ardalić, Daniela
AU  - Spasojević-Kalimanovska, Vesna
AU  - Ivanišević, Jasmina
AU  - Gojković, Tamara
AU  - Vladimirov, Sandra
AU  - Mihajlović, Marija
AU  - Antonić, Tamara
AU  - Vujčić, Sanja
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4487
AB  - Insulin resistance, hyperlipidemia, oxidative stress and low-grade inflammation are
mechanisms underlying endothelial dysfunction and atherosclerosis development in non-
pregnant state, but in pregnancy they are adaptive mechanisms responsible for fetal
development without health risks for mothers. A special feature of lipids profile in pregnancy
is increase in high density lipoprotein (HDL) cholesterol concentration. Protective role of
HDL particles resides on its complex composition that goes beyond its cholesterol
concentration and exhibits vasodilatory, antioxidant, anti-inflammatory and
antithrombogenic effects. Even the importance of HDL functionality in pathogenesis of
cardiovascular disease has been investigated and confirmed its role in persevering metabolic
health in pregnancy are almost unexplored. Project "HDL research to improve pregnancy
outcome" investigate the pattern of HDL particle distribution and functionality (HDL-ome)
with different pregnancy outcomes. With the sophisticated analytical methods we determine
HDL-ome in uncomplicated and high-risk pregnancies. We mark the HDL-ome component
with a different pattern of change during high-risk pregnancies and test their potential for
pregnancy complications risk assessment. In this this research we investigate molecular
mechanisms crucial for HDL remodelling during pregnancy and possible associations
between maternal metabolic health during pregnancy and maternal and child health in the
future. Such comprehensive approach will provide a better insight into HDL role in maternal
metabolic health during pregnancy and will open a door for further research in this area.
AB  - Insulinska rezistencija, hiperlipidemija, oksidativni stress i stanje inflamacije niskog
stepena, koji se u negravidnom stanju povezuju sa razvojem endotelne disfunkcije i
aterosklerozom, u trudnoći predstavljaju adaptivne mehanizme koji omogućavaju pravilan
rast i razvoj fetusa, a ne ugrožavaju zdravlje trudnice. Specifičnost dislipidemije koja se
razvija u trudnoći, a koja nije karakteristična za dislipidemiju u negravidnim stanjima, jeste
porast koncentracije holesterola u lipoproteinskim česticama visoke gustine (high density
lipoprotein, HDL). Međutim, HDL je mnogo više od sadržaja holesterola u njemu. Ovaj
lipoprotein ima izuzetno kompleksnu i heterogenu strukturu i ispoljava vazodilatatorne,
antioksidativne, antiinflamatorne i antitrombogene efekte. Iako je gubitak funkcionalnosti
ovog lipoproteina u patogenezi kardiovaskularnih bolesti detaljno ispitan i potvrđen,
njegova uloga u očuvanju metaboličkog zdravlja u trudnoći još uvek nije rasvetljena. Projekat
"Ispitivanje HDL-metaboloma u cilju unapređenja ishoda trudnoće" bavi se istraživanjem
povezanosti obrazaca promena strukture i funkcionalnosti HDL (HDL-om) sa različitim
ishodima trudnoće. Sofisticiranim analitičkim metodama, određuje se HDL-om u
visokorizičnim trudnoćama, kao i trudnoćama bez komplikacija. Izdvajaju se komponente
HDL-oma koje imaju drugačiji obrazac promene tokom visoko rizičnih trudnoća i ispituje se
njihov značaj u proceni rizika za razvoj komplikacija u trudnoći. Poseban aspekt ovog
istraživanja odnosi se na ispitivanje molekularnih mehanizama ključnih za remodelovanje
HDL tokom trudnoće, kao i potencijalne povezanosti metaboličkog statusa majke tokom
trudnoće sa budućim kardiometaboličkim zdravljem majke i deteta. Ovako opsežno
ispitivanje trebalo bi da pruži jasniji prikaz uloge HDL u očuvanju metaboličkog zdravlja
tokom trudnoće i, kao takvo, predstavljaće polaznu tačku za dalja istraživanja u ovoj oblasti.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - The importance of metabolic health improvement in pregnancy: Project "HDL structure and function research in pregnancy"
T1  - Značaj očuvanja metaboličkog zdravlja u trudnoći: projekat "ispitivanje strukture i funkcije hdl‐a u trudnoći"
VL  - 72
IS  - 4 suplement
SP  - S174
EP  - S175
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4487
ER  - 

@conference{
author = "Zeljković, Aleksandra and Vekić, Jelena and Ardalić, Daniela and Spasojević-Kalimanovska, Vesna and Ivanišević, Jasmina and Gojković, Tamara and Vladimirov, Sandra and Mihajlović, Marija and Antonić, Tamara and Vujčić, Sanja and Miković, Željko and Stefanović, Aleksandra",
year = "2022",
abstract = "Insulin resistance, hyperlipidemia, oxidative stress and low-grade inflammation are
mechanisms underlying endothelial dysfunction and atherosclerosis development in non-
pregnant state, but in pregnancy they are adaptive mechanisms responsible for fetal
development without health risks for mothers. A special feature of lipids profile in pregnancy
is increase in high density lipoprotein (HDL) cholesterol concentration. Protective role of
HDL particles resides on its complex composition that goes beyond its cholesterol
concentration and exhibits vasodilatory, antioxidant, anti-inflammatory and
antithrombogenic effects. Even the importance of HDL functionality in pathogenesis of
cardiovascular disease has been investigated and confirmed its role in persevering metabolic
health in pregnancy are almost unexplored. Project "HDL research to improve pregnancy
outcome" investigate the pattern of HDL particle distribution and functionality (HDL-ome)
with different pregnancy outcomes. With the sophisticated analytical methods we determine
HDL-ome in uncomplicated and high-risk pregnancies. We mark the HDL-ome component
with a different pattern of change during high-risk pregnancies and test their potential for
pregnancy complications risk assessment. In this this research we investigate molecular
mechanisms crucial for HDL remodelling during pregnancy and possible associations
between maternal metabolic health during pregnancy and maternal and child health in the
future. Such comprehensive approach will provide a better insight into HDL role in maternal
metabolic health during pregnancy and will open a door for further research in this area., Insulinska rezistencija, hiperlipidemija, oksidativni stress i stanje inflamacije niskog
stepena, koji se u negravidnom stanju povezuju sa razvojem endotelne disfunkcije i
aterosklerozom, u trudnoći predstavljaju adaptivne mehanizme koji omogućavaju pravilan
rast i razvoj fetusa, a ne ugrožavaju zdravlje trudnice. Specifičnost dislipidemije koja se
razvija u trudnoći, a koja nije karakteristična za dislipidemiju u negravidnim stanjima, jeste
porast koncentracije holesterola u lipoproteinskim česticama visoke gustine (high density
lipoprotein, HDL). Međutim, HDL je mnogo više od sadržaja holesterola u njemu. Ovaj
lipoprotein ima izuzetno kompleksnu i heterogenu strukturu i ispoljava vazodilatatorne,
antioksidativne, antiinflamatorne i antitrombogene efekte. Iako je gubitak funkcionalnosti
ovog lipoproteina u patogenezi kardiovaskularnih bolesti detaljno ispitan i potvrđen,
njegova uloga u očuvanju metaboličkog zdravlja u trudnoći još uvek nije rasvetljena. Projekat
"Ispitivanje HDL-metaboloma u cilju unapređenja ishoda trudnoće" bavi se istraživanjem
povezanosti obrazaca promena strukture i funkcionalnosti HDL (HDL-om) sa različitim
ishodima trudnoće. Sofisticiranim analitičkim metodama, određuje se HDL-om u
visokorizičnim trudnoćama, kao i trudnoćama bez komplikacija. Izdvajaju se komponente
HDL-oma koje imaju drugačiji obrazac promene tokom visoko rizičnih trudnoća i ispituje se
njihov značaj u proceni rizika za razvoj komplikacija u trudnoći. Poseban aspekt ovog
istraživanja odnosi se na ispitivanje molekularnih mehanizama ključnih za remodelovanje
HDL tokom trudnoće, kao i potencijalne povezanosti metaboličkog statusa majke tokom
trudnoće sa budućim kardiometaboličkim zdravljem majke i deteta. Ovako opsežno
ispitivanje trebalo bi da pruži jasniji prikaz uloge HDL u očuvanju metaboličkog zdravlja
tokom trudnoće i, kao takvo, predstavljaće polaznu tačku za dalja istraživanja u ovoj oblasti.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "The importance of metabolic health improvement in pregnancy: Project "HDL structure and function research in pregnancy", Značaj očuvanja metaboličkog zdravlja u trudnoći: projekat "ispitivanje strukture i funkcije hdl‐a u trudnoći"",
volume = "72",
number = "4 suplement",
pages = "S174-S175",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4487"
}

Zeljković, A., Vekić, J., Ardalić, D., Spasojević-Kalimanovska, V., Ivanišević, J., Gojković, T., Vladimirov, S., Mihajlović, M., Antonić, T., Vujčić, S., Miković, Ž.,& Stefanović, A.. (2022). The importance of metabolic health improvement in pregnancy: Project "HDL structure and function research in pregnancy". in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S174-S175.
https://hdl.handle.net/21.15107/rcub_farfar_4487

Zeljković A, Vekić J, Ardalić D, Spasojević-Kalimanovska V, Ivanišević J, Gojković T, Vladimirov S, Mihajlović M, Antonić T, Vujčić S, Miković Ž, Stefanović A. The importance of metabolic health improvement in pregnancy: Project "HDL structure and function research in pregnancy". in Arhiv za farmaciju. 2022;72(4 suplement):S174-S175.
https://hdl.handle.net/21.15107/rcub_farfar_4487 .

Zeljković, Aleksandra, Vekić, Jelena, Ardalić, Daniela, Spasojević-Kalimanovska, Vesna, Ivanišević, Jasmina, Gojković, Tamara, Vladimirov, Sandra, Mihajlović, Marija, Antonić, Tamara, Vujčić, Sanja, Miković, Željko, Stefanović, Aleksandra, "The importance of metabolic health improvement in pregnancy: Project "HDL structure and function research in pregnancy"" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S174-S175,
https://hdl.handle.net/21.15107/rcub_farfar_4487 .

TY  - JOUR
AU  - Antonić, Tamara
AU  - Ardalić, Daniela
AU  - Vladimirov, Sandra
AU  - Banjac, Gorica
AU  - Cabunac, Petar
AU  - Zeljković, Aleksandra
AU  - Karadžov-Orlić, Natasa
AU  - Spasojević-Kalimanovska, Vesna
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4023
AB  - Introduction The link between preeclampsia and dyslipidemia has been established. Even though lipid profile parameters have been intensively investigated in the pathology of preeclampsia, their accurate molecular mechanisms of action have not been fully decoded. Objectives We aimed to identify the specifics of cholesterol metabolism in women affected by late‑onset preeclampsia and single out potential biomarkers associated with late‑onset syndrome. Patient s and methods A total of 90 pregnant women with a priori risk for preeclampsia were monitored at 4 time points during gestation and, based on the outcome of pregnancy, they were classified into the high‑risk group (70 women) and the preeclampsia group (20 women). Cholesterol metabolic profiling was done using liquid chromatography‑tandem mass spectrometry. Result s The only significant change in the preeclampsia group was an increase in the lathosterol level (P = 0.001). The first‑trimester lathosterol level was higher in the preeclampsia group compared with the high‑risk group (P = 0.02). Further, in the preeclampsia group, positive correlations were found between desmosterol and β‑sitosterol (ρ = 0.474; P = 0.03) in the third trimester, desmosterol and campesterol changes between the second and the first (ρ = 0.546; P = 0.02), and the third and first trimesters (ρ = 0.754; P <0.001), as well as between the desmosterol and β‑sitosterol differences between the third and first trimesters (ρ = 0.568; P = 0.01). No similar correlations were found in the high‑risk group. Conclusions Late‑onset preeclampsia could be associated with an altered lipid profile. By studying the quantitative metabolic signatures of cholesterol, we might assume that both cholesterol synthesis and absorption are increased, that is, there is an imbalance in the cholesterol homeostasis regulation in women affected by the disease.
PB  - Medycyna Praktyczna Cholerzyn
T2  - Polish Archives of Internal Medicine
T1  - Cholesterol homeostasis is dysregulated in women with preeclampsia
VL  - 131
IS  - 12
DO  - 10.20452/pamw.16144
ER  - 

@article{
author = "Antonić, Tamara and Ardalić, Daniela and Vladimirov, Sandra and Banjac, Gorica and Cabunac, Petar and Zeljković, Aleksandra and Karadžov-Orlić, Natasa and Spasojević-Kalimanovska, Vesna and Miković, Željko and Stefanović, Aleksandra",
year = "2021",
abstract = "Introduction The link between preeclampsia and dyslipidemia has been established. Even though lipid profile parameters have been intensively investigated in the pathology of preeclampsia, their accurate molecular mechanisms of action have not been fully decoded. Objectives We aimed to identify the specifics of cholesterol metabolism in women affected by late‑onset preeclampsia and single out potential biomarkers associated with late‑onset syndrome. Patient s and methods A total of 90 pregnant women with a priori risk for preeclampsia were monitored at 4 time points during gestation and, based on the outcome of pregnancy, they were classified into the high‑risk group (70 women) and the preeclampsia group (20 women). Cholesterol metabolic profiling was done using liquid chromatography‑tandem mass spectrometry. Result s The only significant change in the preeclampsia group was an increase in the lathosterol level (P = 0.001). The first‑trimester lathosterol level was higher in the preeclampsia group compared with the high‑risk group (P = 0.02). Further, in the preeclampsia group, positive correlations were found between desmosterol and β‑sitosterol (ρ = 0.474; P = 0.03) in the third trimester, desmosterol and campesterol changes between the second and the first (ρ = 0.546; P = 0.02), and the third and first trimesters (ρ = 0.754; P <0.001), as well as between the desmosterol and β‑sitosterol differences between the third and first trimesters (ρ = 0.568; P = 0.01). No similar correlations were found in the high‑risk group. Conclusions Late‑onset preeclampsia could be associated with an altered lipid profile. By studying the quantitative metabolic signatures of cholesterol, we might assume that both cholesterol synthesis and absorption are increased, that is, there is an imbalance in the cholesterol homeostasis regulation in women affected by the disease.",
publisher = "Medycyna Praktyczna Cholerzyn",
journal = "Polish Archives of Internal Medicine",
title = "Cholesterol homeostasis is dysregulated in women with preeclampsia",
volume = "131",
number = "12",
doi = "10.20452/pamw.16144"
}

Antonić, T., Ardalić, D., Vladimirov, S., Banjac, G., Cabunac, P., Zeljković, A., Karadžov-Orlić, N., Spasojević-Kalimanovska, V., Miković, Ž.,& Stefanović, A.. (2021). Cholesterol homeostasis is dysregulated in women with preeclampsia. in Polish Archives of Internal Medicine
Medycyna Praktyczna Cholerzyn., 131(12).
https://doi.org/10.20452/pamw.16144

Antonić T, Ardalić D, Vladimirov S, Banjac G, Cabunac P, Zeljković A, Karadžov-Orlić N, Spasojević-Kalimanovska V, Miković Ž, Stefanović A. Cholesterol homeostasis is dysregulated in women with preeclampsia. in Polish Archives of Internal Medicine. 2021;131(12).
doi:10.20452/pamw.16144 .

Antonić, Tamara, Ardalić, Daniela, Vladimirov, Sandra, Banjac, Gorica, Cabunac, Petar, Zeljković, Aleksandra, Karadžov-Orlić, Natasa, Spasojević-Kalimanovska, Vesna, Miković, Željko, Stefanović, Aleksandra, "Cholesterol homeostasis is dysregulated in women with preeclampsia" in Polish Archives of Internal Medicine, 131, no. 12 (2021),
https://doi.org/10.20452/pamw.16144 . .

TY  - CONF
AU  - Ivanišević, Jasmina
AU  - Ardalić, Daniela
AU  - Banjac, Gorica
AU  - Janać, Jelena
AU  - Cabunac, Petar
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Spasojević-Kalimanovska, Vesna
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4361
PB  - Elsevier B.V.
C3  - Free Radical Biology and Medicine
T1  - Antioxidant status in hypertensive disorders of pregnancy
VL  - 177
IS  - Supplement 1
SP  - S116
EP  - S117
DO  - 10.1016/j.freeradbiomed.2021.08.185
ER  - 

@conference{
author = "Ivanišević, Jasmina and Ardalić, Daniela and Banjac, Gorica and Janać, Jelena and Cabunac, Petar and Vekić, Jelena and Zeljković, Aleksandra and Spasojević-Kalimanovska, Vesna and Miković, Željko and Stefanović, Aleksandra",
year = "2021",
publisher = "Elsevier B.V.",
journal = "Free Radical Biology and Medicine",
title = "Antioxidant status in hypertensive disorders of pregnancy",
volume = "177",
number = "Supplement 1",
pages = "S116-S117",
doi = "10.1016/j.freeradbiomed.2021.08.185"
}

Ivanišević, J., Ardalić, D., Banjac, G., Janać, J., Cabunac, P., Vekić, J., Zeljković, A., Spasojević-Kalimanovska, V., Miković, Ž.,& Stefanović, A.. (2021). Antioxidant status in hypertensive disorders of pregnancy. in Free Radical Biology and Medicine
Elsevier B.V.., 177(Supplement 1), S116-S117.
https://doi.org/10.1016/j.freeradbiomed.2021.08.185

Ivanišević J, Ardalić D, Banjac G, Janać J, Cabunac P, Vekić J, Zeljković A, Spasojević-Kalimanovska V, Miković Ž, Stefanović A. Antioxidant status in hypertensive disorders of pregnancy. in Free Radical Biology and Medicine. 2021;177(Supplement 1):S116-S117.
doi:10.1016/j.freeradbiomed.2021.08.185 .

Ivanišević, Jasmina, Ardalić, Daniela, Banjac, Gorica, Janać, Jelena, Cabunac, Petar, Vekić, Jelena, Zeljković, Aleksandra, Spasojević-Kalimanovska, Vesna, Miković, Željko, Stefanović, Aleksandra, "Antioxidant status in hypertensive disorders of pregnancy" in Free Radical Biology and Medicine, 177, no. Supplement 1 (2021):S116-S117,
https://doi.org/10.1016/j.freeradbiomed.2021.08.185 . .

TY  - JOUR
AU  - Ardalić, Daniela
AU  - Stefanović, Aleksandra
AU  - Banjac, Gorica
AU  - Cabunac, Petar
AU  - Miljković, Milica
AU  - Mandić-Marković, Vesna
AU  - Stanimirović, Srđan
AU  - Damnjanović Pažin, Barbara
AU  - Spasić, Slavíca
AU  - Spasojević-Kalimanovska, Vesna
AU  - Karadžov-Orlić, Nataša
AU  - Miković, Željko
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3626
AB  - Objectives: The goal of this study was to investigate metabolic changes in lipids and oxidative stress parameters in the first trimester of pregnancy with the more specific aim of estimating the significance and strength of researched parameters in the prediction of preeclampsia. Design and Methods. The study included 87 high-risk pregnant (HRG) female subjects, 14 with developed preeclampsia (PEC) and 43 healthy pregnant female subjects matched for gestational age (CG). Thiobarbituric acid-reactive substances (TBARS) concentration, lipid hydroperoxides (LOOH), pro-oxidant antioxidant balance (PAB) and total oxidative status (TOS) were measured as oxidative stress markers, while total antioxidant capacity (TAC) was measured as an antioxidative defense parameter. The Atherogenic Index of Plasma (AIP) was calculated as the base 10 logarithm of the ratio of the plasma concentration of triglycerides (TG) to the plasma concentration of highdensity lipoprotein cholesterol (HDL-C), with each concentration expressed in mmol/L. Results: The results have shown that lipid indices, especially AIP, were significantly higher in the first trimester of HRG (p < 0.001) and PEC (p < 0.001). Oxidative stress parameters were significantlly higher, while TAC was significantly lower in HRG vs. CG [0.7 ± 0.15 vs 1.1 ± 0.16; (p < 0.001)] and in PEC [0.6 ± 0.12 vs 1.1 ± 0.16; (p < 0.001)] vs. CG. Also, in the HRG, results have shown an independent association of AIP with the preeclampsia development (p < 0.05), while placental growth factor did not show the expected level of significance (p = 0.648). Analysis of the Receiver Operating Characteristics (ROC) curves indicated that certain parameters included in the research model have very good diagnostic accuracy for preeclampsia (AUC = 0.856). Conclusions: AIP is associated with high-risk pregnancies. Furthermore, our results firmly underscored AIP as a potential marker for preeclampsia prediction.
PB  - Elsevier
T2  - Clinical Biochemistry
T1  - Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction
VL  - 81
SP  - 34
EP  - 40
DO  - 10.1016/j.clinbiochem.2020.05.003
ER  - 

@article{
author = "Ardalić, Daniela and Stefanović, Aleksandra and Banjac, Gorica and Cabunac, Petar and Miljković, Milica and Mandić-Marković, Vesna and Stanimirović, Srđan and Damnjanović Pažin, Barbara and Spasić, Slavíca and Spasojević-Kalimanovska, Vesna and Karadžov-Orlić, Nataša and Miković, Željko",
year = "2020",
abstract = "Objectives: The goal of this study was to investigate metabolic changes in lipids and oxidative stress parameters in the first trimester of pregnancy with the more specific aim of estimating the significance and strength of researched parameters in the prediction of preeclampsia. Design and Methods. The study included 87 high-risk pregnant (HRG) female subjects, 14 with developed preeclampsia (PEC) and 43 healthy pregnant female subjects matched for gestational age (CG). Thiobarbituric acid-reactive substances (TBARS) concentration, lipid hydroperoxides (LOOH), pro-oxidant antioxidant balance (PAB) and total oxidative status (TOS) were measured as oxidative stress markers, while total antioxidant capacity (TAC) was measured as an antioxidative defense parameter. The Atherogenic Index of Plasma (AIP) was calculated as the base 10 logarithm of the ratio of the plasma concentration of triglycerides (TG) to the plasma concentration of highdensity lipoprotein cholesterol (HDL-C), with each concentration expressed in mmol/L. Results: The results have shown that lipid indices, especially AIP, were significantly higher in the first trimester of HRG (p < 0.001) and PEC (p < 0.001). Oxidative stress parameters were significantlly higher, while TAC was significantly lower in HRG vs. CG [0.7 ± 0.15 vs 1.1 ± 0.16; (p < 0.001)] and in PEC [0.6 ± 0.12 vs 1.1 ± 0.16; (p < 0.001)] vs. CG. Also, in the HRG, results have shown an independent association of AIP with the preeclampsia development (p < 0.05), while placental growth factor did not show the expected level of significance (p = 0.648). Analysis of the Receiver Operating Characteristics (ROC) curves indicated that certain parameters included in the research model have very good diagnostic accuracy for preeclampsia (AUC = 0.856). Conclusions: AIP is associated with high-risk pregnancies. Furthermore, our results firmly underscored AIP as a potential marker for preeclampsia prediction.",
publisher = "Elsevier",
journal = "Clinical Biochemistry",
title = "Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction",
volume = "81",
pages = "34-40",
doi = "10.1016/j.clinbiochem.2020.05.003"
}

Ardalić, D., Stefanović, A., Banjac, G., Cabunac, P., Miljković, M., Mandić-Marković, V., Stanimirović, S., Damnjanović Pažin, B., Spasić, S., Spasojević-Kalimanovska, V., Karadžov-Orlić, N.,& Miković, Ž.. (2020). Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction. in Clinical Biochemistry
Elsevier., 81, 34-40.
https://doi.org/10.1016/j.clinbiochem.2020.05.003

Ardalić D, Stefanović A, Banjac G, Cabunac P, Miljković M, Mandić-Marković V, Stanimirović S, Damnjanović Pažin B, Spasić S, Spasojević-Kalimanovska V, Karadžov-Orlić N, Miković Ž. Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction. in Clinical Biochemistry. 2020;81:34-40.
doi:10.1016/j.clinbiochem.2020.05.003 .

Ardalić, Daniela, Stefanović, Aleksandra, Banjac, Gorica, Cabunac, Petar, Miljković, Milica, Mandić-Marković, Vesna, Stanimirović, Srđan, Damnjanović Pažin, Barbara, Spasić, Slavíca, Spasojević-Kalimanovska, Vesna, Karadžov-Orlić, Nataša, Miković, Željko, "Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction" in Clinical Biochemistry, 81 (2020):34-40,
https://doi.org/10.1016/j.clinbiochem.2020.05.003 . .

TY  - JOUR
AU  - Ardalić, Daniela
AU  - Stefanović, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Ninić, Ana
AU  - Spasić, Slavica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Jelić-Ivanović, Zorana
AU  - Miković, Željko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3370
AB  - Background: Specific metabolic changes during physiological pregnancy are characterized by hyperlipidemia and increased oxidative stress. However, these specific changes raise the question of their pro-atherogenic potential during pregnancy and their influence on the risk of developing cardiovascular disease (CVD) in women later in life, as well as complications during pregnancy. The aim of this study was to investigate the changes in lipid indexes and parameters of lipid peroxidation in non-complicated pregnancy in order to estimate their course of change and potential relationship during non-complicated pregnancy. Methods: The study included 43 healthy pregnant women and 38 non-pregnant healthy women, in appropriate ages, as the control group. Lipid parameters and oxidative stress parameters were monitored in a longitudinal study in the first, second and third trimesters, and before delivery during non-complicated pregnancy. Results: Results have shown that lipid indexes rise during pregnancy. The values were significantly increased when compared to the first trimester in all lipid indices and in comparison with the control group. Thyobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (LOOH) were not changed significantly during physiological pregnancy, but LOOH showed a significantly higher value in the first trimester compared with the control group. Prooxidative-antioxidative balance (PAB) significantly increases as pregnancy progresses. Conclusions: We observed the changes in lipids, lipid indexes and parameters that indicate oxidative modification of lipids in physiological pregnancy that may lead to an atherogenic, prooxidant state.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Journal of Laboratory Medicine
T1  - Lipid indexes and parameters of lipid peroxidation during physiological pregnancy
VL  - 43
IS  - 2
SP  - 93
EP  - 99
DO  - 10.1515/labmed-2018-0026
ER  - 

@article{
author = "Ardalić, Daniela and Stefanović, Aleksandra and Kotur-Stevuljević, Jelena and Ninić, Ana and Spasić, Slavica and Spasojević-Kalimanovska, Vesna and Jelić-Ivanović, Zorana and Miković, Željko",
year = "2019",
abstract = "Background: Specific metabolic changes during physiological pregnancy are characterized by hyperlipidemia and increased oxidative stress. However, these specific changes raise the question of their pro-atherogenic potential during pregnancy and their influence on the risk of developing cardiovascular disease (CVD) in women later in life, as well as complications during pregnancy. The aim of this study was to investigate the changes in lipid indexes and parameters of lipid peroxidation in non-complicated pregnancy in order to estimate their course of change and potential relationship during non-complicated pregnancy. Methods: The study included 43 healthy pregnant women and 38 non-pregnant healthy women, in appropriate ages, as the control group. Lipid parameters and oxidative stress parameters were monitored in a longitudinal study in the first, second and third trimesters, and before delivery during non-complicated pregnancy. Results: Results have shown that lipid indexes rise during pregnancy. The values were significantly increased when compared to the first trimester in all lipid indices and in comparison with the control group. Thyobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (LOOH) were not changed significantly during physiological pregnancy, but LOOH showed a significantly higher value in the first trimester compared with the control group. Prooxidative-antioxidative balance (PAB) significantly increases as pregnancy progresses. Conclusions: We observed the changes in lipids, lipid indexes and parameters that indicate oxidative modification of lipids in physiological pregnancy that may lead to an atherogenic, prooxidant state.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Journal of Laboratory Medicine",
title = "Lipid indexes and parameters of lipid peroxidation during physiological pregnancy",
volume = "43",
number = "2",
pages = "93-99",
doi = "10.1515/labmed-2018-0026"
}

Ardalić, D., Stefanović, A., Kotur-Stevuljević, J., Ninić, A., Spasić, S., Spasojević-Kalimanovska, V., Jelić-Ivanović, Z.,& Miković, Ž.. (2019). Lipid indexes and parameters of lipid peroxidation during physiological pregnancy. in Journal of Laboratory Medicine
Walter de Gruyter Gmbh, Berlin., 43(2), 93-99.
https://doi.org/10.1515/labmed-2018-0026

Ardalić D, Stefanović A, Kotur-Stevuljević J, Ninić A, Spasić S, Spasojević-Kalimanovska V, Jelić-Ivanović Z, Miković Ž. Lipid indexes and parameters of lipid peroxidation during physiological pregnancy. in Journal of Laboratory Medicine. 2019;43(2):93-99.
doi:10.1515/labmed-2018-0026 .

Ardalić, Daniela, Stefanović, Aleksandra, Kotur-Stevuljević, Jelena, Ninić, Ana, Spasić, Slavica, Spasojević-Kalimanovska, Vesna, Jelić-Ivanović, Zorana, Miković, Željko, "Lipid indexes and parameters of lipid peroxidation during physiological pregnancy" in Journal of Laboratory Medicine, 43, no. 2 (2019):93-99,
https://doi.org/10.1515/labmed-2018-0026 . .

TY  - JOUR
AU  - Ardalić, Daniela
AU  - Stefanović, Aleksandra
AU  - Spasić, Slavica
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Spasojević-Kalimanovska, Vesna
AU  - Jelić-Ivanović, Zorana
AU  - Mandić-Marković, Vesna
AU  - Miković, Željko
AU  - Cerović, Nikola
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2699
AB  - Specifically altered lipid profile and physiological hyperlipidemia during pregnancy are considered essential for the normal course of pregnancy and fetal development. This specific alteration of the lipid profile raises the questions about potential proaterogenic effect of these altered lipid parameters during pregnancy and its influence on the development of cardiovascular disease in women later in life. Research topic was also the association of altered lipid profile during pregnancy with the development of complications in pregnancy, especially gestational diabetes, hypertension and preeclampsia. Through the mediation of cholesterol ester transfer protein (CETP), the activity of which grows in mid-gestation, there are exchanges of the triglycerides between VLDL and LDL or HDL particle, which leads to increased accumulation of triglycerides in these particles, causes them to become smaller and denser with much greater atherogenic potential. These changes in lipid profile point out that a large number of pregnancies increase risk of development of cardiovascular diseases later in life. In order to optimize the predictive capacity of the lipid profile during pregnancy, it is recommended to determine the indexes of lipid.
AB  - Specifično izmenjen lipidni profil i fiziološka hiperlipidemija tokom trudnoće smatraju se esencijalnim za normalan ishod trudnoće i razvoj fetusa, a upravo ova specifičnost promena parametara lipidnog profila otvara pitanja njihove potencijalne proaterogenosti tokom trudnoće i uticaja na rizik za razvoj kardiovaskularne bolesti u kasnijem životu žene. Takođe se ispituje i povezanost izmenjenog lipidnog profila tokom trudnoće sa razvojem komplikacija u trudnoći, pre svega sa razvojem gestacijskog dijabetesa, hipertenzije i preeklampsije. Posredovanjem holesterol estar transportnog proteina (CETP), čija aktivnost raste sredinom trudnoće, dolazi do izmene triglicerida i holesterola između lipoproteinskih čestica vrlo niske gustine (VLDL) i lipoproteinskih čestica male gustine (LDL) ili lipoproteinskih čestica velike gustine (HDL), tako da dolazi do povećanog nakupljanja triglicerida u LDL i HDL česticama, pri čemu one postaju manje i gušće, i kao takve imaju veći proaterogeni potencijal, što sve ukazuje i na proaterogeni potencijal trudnoće, posebno ukoliko se ona ponavlja više puta tokom života. Da bi se optimizirao prediktivni kapacitet lipidnog profila tokom trudnoće preporučuje se određivanje lipidnih indeksa.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Lipid status in phyisiological non-complicated pregnancy
T1  - Lipidni status fiziološke nekomplikovane trudnoće
VL  - 66
IS  - 5
SP  - 191
EP  - 206
DO  - 10.5937/arhfarm1605191A
ER  - 

@article{
author = "Ardalić, Daniela and Stefanović, Aleksandra and Spasić, Slavica and Zeljković, Aleksandra and Vekić, Jelena and Spasojević-Kalimanovska, Vesna and Jelić-Ivanović, Zorana and Mandić-Marković, Vesna and Miković, Željko and Cerović, Nikola",
year = "2016",
abstract = "Specifically altered lipid profile and physiological hyperlipidemia during pregnancy are considered essential for the normal course of pregnancy and fetal development. This specific alteration of the lipid profile raises the questions about potential proaterogenic effect of these altered lipid parameters during pregnancy and its influence on the development of cardiovascular disease in women later in life. Research topic was also the association of altered lipid profile during pregnancy with the development of complications in pregnancy, especially gestational diabetes, hypertension and preeclampsia. Through the mediation of cholesterol ester transfer protein (CETP), the activity of which grows in mid-gestation, there are exchanges of the triglycerides between VLDL and LDL or HDL particle, which leads to increased accumulation of triglycerides in these particles, causes them to become smaller and denser with much greater atherogenic potential. These changes in lipid profile point out that a large number of pregnancies increase risk of development of cardiovascular diseases later in life. In order to optimize the predictive capacity of the lipid profile during pregnancy, it is recommended to determine the indexes of lipid., Specifično izmenjen lipidni profil i fiziološka hiperlipidemija tokom trudnoće smatraju se esencijalnim za normalan ishod trudnoće i razvoj fetusa, a upravo ova specifičnost promena parametara lipidnog profila otvara pitanja njihove potencijalne proaterogenosti tokom trudnoće i uticaja na rizik za razvoj kardiovaskularne bolesti u kasnijem životu žene. Takođe se ispituje i povezanost izmenjenog lipidnog profila tokom trudnoće sa razvojem komplikacija u trudnoći, pre svega sa razvojem gestacijskog dijabetesa, hipertenzije i preeklampsije. Posredovanjem holesterol estar transportnog proteina (CETP), čija aktivnost raste sredinom trudnoće, dolazi do izmene triglicerida i holesterola između lipoproteinskih čestica vrlo niske gustine (VLDL) i lipoproteinskih čestica male gustine (LDL) ili lipoproteinskih čestica velike gustine (HDL), tako da dolazi do povećanog nakupljanja triglicerida u LDL i HDL česticama, pri čemu one postaju manje i gušće, i kao takve imaju veći proaterogeni potencijal, što sve ukazuje i na proaterogeni potencijal trudnoće, posebno ukoliko se ona ponavlja više puta tokom života. Da bi se optimizirao prediktivni kapacitet lipidnog profila tokom trudnoće preporučuje se određivanje lipidnih indeksa.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Lipid status in phyisiological non-complicated pregnancy, Lipidni status fiziološke nekomplikovane trudnoće",
volume = "66",
number = "5",
pages = "191-206",
doi = "10.5937/arhfarm1605191A"
}

Ardalić, D., Stefanović, A., Spasić, S., Zeljković, A., Vekić, J., Spasojević-Kalimanovska, V., Jelić-Ivanović, Z., Mandić-Marković, V., Miković, Ž.,& Cerović, N.. (2016). Lipid status in phyisiological non-complicated pregnancy. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 66(5), 191-206.
https://doi.org/10.5937/arhfarm1605191A

Ardalić D, Stefanović A, Spasić S, Zeljković A, Vekić J, Spasojević-Kalimanovska V, Jelić-Ivanović Z, Mandić-Marković V, Miković Ž, Cerović N. Lipid status in phyisiological non-complicated pregnancy. in Arhiv za farmaciju. 2016;66(5):191-206.
doi:10.5937/arhfarm1605191A .

Ardalić, Daniela, Stefanović, Aleksandra, Spasić, Slavica, Zeljković, Aleksandra, Vekić, Jelena, Spasojević-Kalimanovska, Vesna, Jelić-Ivanović, Zorana, Mandić-Marković, Vesna, Miković, Željko, Cerović, Nikola, "Lipid status in phyisiological non-complicated pregnancy" in Arhiv za farmaciju, 66, no. 5 (2016):191-206,
https://doi.org/10.5937/arhfarm1605191A . .

TY  - JOUR
AU  - Ardalić, Daniela
AU  - Stefanović, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Vujović, Ana
AU  - Spasić, Slavica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Jelić-Ivanović, Zorana
AU  - Mandić-Marković, Vesna
AU  - Miković, Željko
AU  - Cerović, Nikola
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2143
AB  - Background. As a physiological condition closely linked with increased susceptibility to oxidative stress, pregnancy can be further compromised by cigarette smoking. Inadequate nutrition and reduced intake of antioxidants can also disrupt the prooxidant/antioxidant relationship and contribute to oxidative stress. Increased oxidative stress during pregnancy may be involved in several complications of pregnancy, such as preterm labor, fetal growth restriction, preeclampsia and Miscarriage. Objectives. The aim of this study was to investigate the influence of maternal smoking habits before pregnancy on the parameters of oxidative stress and the antioxidative defense system, lipid profile parameters and paraoxonase-1 (PON1) activity during the third trimester of uncomplicated pregnancies. Material and Methods. Healthy pregnant women (n = 86) were divided into non-smoking and smoking groups, and into groups taking vitamin supplements and not taking them. Oxidative damage was measured through the levels of thiobarbituric acid-reacting substances (TBARS) and plasma antioxidant status was evaluated by measuring total antioxidant capacity (TAC). Results. TBARS concetration was significantly higher (p  lt  0.05) and PON1 activity was significantly lower (p  lt  0.05) in the smokers' group. No significant differences were found in the investigated parameters in relation to vitamin supplement intake. Conclusions. Habitual smoking before pregnancy is associated with increased oxidative stress. Vitamin supplementation has no effect on the oxidative stress status of healthy pregnant women.
PB  - Wroclaw Medical Univ, Wroclaw
T2  - Advances in Clinical and Experimental Medicine
T1  - The Influence of Maternal Smoking Habits Before Pregnancy and Antioxidative Supplementation During Pregnancy on Oxidative Stress Status in a Non-Complicated Pregnancy
VL  - 23
IS  - 4
SP  - 575
EP  - 583
DO  - 10.17219/acem/37226
ER  - 

@article{
author = "Ardalić, Daniela and Stefanović, Aleksandra and Kotur-Stevuljević, Jelena and Vujović, Ana and Spasić, Slavica and Spasojević-Kalimanovska, Vesna and Jelić-Ivanović, Zorana and Mandić-Marković, Vesna and Miković, Željko and Cerović, Nikola",
year = "2014",
abstract = "Background. As a physiological condition closely linked with increased susceptibility to oxidative stress, pregnancy can be further compromised by cigarette smoking. Inadequate nutrition and reduced intake of antioxidants can also disrupt the prooxidant/antioxidant relationship and contribute to oxidative stress. Increased oxidative stress during pregnancy may be involved in several complications of pregnancy, such as preterm labor, fetal growth restriction, preeclampsia and Miscarriage. Objectives. The aim of this study was to investigate the influence of maternal smoking habits before pregnancy on the parameters of oxidative stress and the antioxidative defense system, lipid profile parameters and paraoxonase-1 (PON1) activity during the third trimester of uncomplicated pregnancies. Material and Methods. Healthy pregnant women (n = 86) were divided into non-smoking and smoking groups, and into groups taking vitamin supplements and not taking them. Oxidative damage was measured through the levels of thiobarbituric acid-reacting substances (TBARS) and plasma antioxidant status was evaluated by measuring total antioxidant capacity (TAC). Results. TBARS concetration was significantly higher (p  lt  0.05) and PON1 activity was significantly lower (p  lt  0.05) in the smokers' group. No significant differences were found in the investigated parameters in relation to vitamin supplement intake. Conclusions. Habitual smoking before pregnancy is associated with increased oxidative stress. Vitamin supplementation has no effect on the oxidative stress status of healthy pregnant women.",
publisher = "Wroclaw Medical Univ, Wroclaw",
journal = "Advances in Clinical and Experimental Medicine",
title = "The Influence of Maternal Smoking Habits Before Pregnancy and Antioxidative Supplementation During Pregnancy on Oxidative Stress Status in a Non-Complicated Pregnancy",
volume = "23",
number = "4",
pages = "575-583",
doi = "10.17219/acem/37226"
}

Ardalić, D., Stefanović, A., Kotur-Stevuljević, J., Vujović, A., Spasić, S., Spasojević-Kalimanovska, V., Jelić-Ivanović, Z., Mandić-Marković, V., Miković, Ž.,& Cerović, N.. (2014). The Influence of Maternal Smoking Habits Before Pregnancy and Antioxidative Supplementation During Pregnancy on Oxidative Stress Status in a Non-Complicated Pregnancy. in Advances in Clinical and Experimental Medicine
Wroclaw Medical Univ, Wroclaw., 23(4), 575-583.
https://doi.org/10.17219/acem/37226

Ardalić D, Stefanović A, Kotur-Stevuljević J, Vujović A, Spasić S, Spasojević-Kalimanovska V, Jelić-Ivanović Z, Mandić-Marković V, Miković Ž, Cerović N. The Influence of Maternal Smoking Habits Before Pregnancy and Antioxidative Supplementation During Pregnancy on Oxidative Stress Status in a Non-Complicated Pregnancy. in Advances in Clinical and Experimental Medicine. 2014;23(4):575-583.
doi:10.17219/acem/37226 .

Ardalić, Daniela, Stefanović, Aleksandra, Kotur-Stevuljević, Jelena, Vujović, Ana, Spasić, Slavica, Spasojević-Kalimanovska, Vesna, Jelić-Ivanović, Zorana, Mandić-Marković, Vesna, Miković, Željko, Cerović, Nikola, "The Influence of Maternal Smoking Habits Before Pregnancy and Antioxidative Supplementation During Pregnancy on Oxidative Stress Status in a Non-Complicated Pregnancy" in Advances in Clinical and Experimental Medicine, 23, no. 4 (2014):575-583,
https://doi.org/10.17219/acem/37226 . .

TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Vekić, Jelena
AU  - Spasić, Slavica
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
AU  - Gojković, Tamara
AU  - Ardalić, Daniela
AU  - Mandić-Marković, Vesna
AU  - Cerović, Nikola
AU  - Miković, Željko
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1918
AB  - Pregnancy is associated with alterations in low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses, but the exact pattern of these variations remains controversial. This study investigates longitudinal changes of plasma LDL and HDL particles distributions during the course of normal pregnancy, as well as associations of maternal LDL and HDL subclasses distributions before delivery with parameters of newborn size. Blood samples were collected from 41 healthy pregnant women throughout entire pregnancy, before delivery and 7 weeks postpartum. LDL and HDL subclasses were determined by gradient gel electrophoresis, while other biochemical parameters were measured by standard laboratory methods. During gestation LDL size significantly decreased (P  lt  0.001), due to reduction in relative proportion of LDL I (P  lt  0.01) and increase of LDL II (P  lt  0.001) and IIIA (P  lt  0.05) subclasses. In the same time, HDL size and proportions of HDL 2a particles significantly decreased (P  lt  0.001), with concomitant increase of HDL 3b and 3c subclasses (P  lt  0.05). Observed alterations were associated with changes in serum triglyceride levels. Rearrangement in LDL subclasses distribution during gestation was transient, while postpartum HDL subclasses distribution remained shifted toward smaller particles. Higher proportion of LDL IVB in maternal plasma before delivery was an independent predictor of smaller birth weights and lengths, while higher proportions of LDL IVB and HDL 2a subclasses were independent determinants of newborns' smaller head circumferences. Routine gestational and prenatal care in otherwise normal pregnancy could be complemented with evaluation of LDL and HDL particles distribution in order to ensure an adequate size of the newborn.
PB  - Springer/Plenum Publishers, New York
T2  - Maternal and Child Health Journal
T1  - Changes in LDL and HDL Subclasses in Normal Pregnancy and Associations with Birth Weight, Birth Length and Head Circumference
VL  - 17
IS  - 3
SP  - 556
EP  - 565
DO  - 10.1007/s10995-012-1031-x
ER  - 

@article{
author = "Zeljković, Aleksandra and Vekić, Jelena and Spasić, Slavica and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna and Gojković, Tamara and Ardalić, Daniela and Mandić-Marković, Vesna and Cerović, Nikola and Miković, Željko",
year = "2013",
abstract = "Pregnancy is associated with alterations in low-density lipoprotein (LDL) and high-density lipoprotein (HDL) subclasses, but the exact pattern of these variations remains controversial. This study investigates longitudinal changes of plasma LDL and HDL particles distributions during the course of normal pregnancy, as well as associations of maternal LDL and HDL subclasses distributions before delivery with parameters of newborn size. Blood samples were collected from 41 healthy pregnant women throughout entire pregnancy, before delivery and 7 weeks postpartum. LDL and HDL subclasses were determined by gradient gel electrophoresis, while other biochemical parameters were measured by standard laboratory methods. During gestation LDL size significantly decreased (P  lt  0.001), due to reduction in relative proportion of LDL I (P  lt  0.01) and increase of LDL II (P  lt  0.001) and IIIA (P  lt  0.05) subclasses. In the same time, HDL size and proportions of HDL 2a particles significantly decreased (P  lt  0.001), with concomitant increase of HDL 3b and 3c subclasses (P  lt  0.05). Observed alterations were associated with changes in serum triglyceride levels. Rearrangement in LDL subclasses distribution during gestation was transient, while postpartum HDL subclasses distribution remained shifted toward smaller particles. Higher proportion of LDL IVB in maternal plasma before delivery was an independent predictor of smaller birth weights and lengths, while higher proportions of LDL IVB and HDL 2a subclasses were independent determinants of newborns' smaller head circumferences. Routine gestational and prenatal care in otherwise normal pregnancy could be complemented with evaluation of LDL and HDL particles distribution in order to ensure an adequate size of the newborn.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Maternal and Child Health Journal",
title = "Changes in LDL and HDL Subclasses in Normal Pregnancy and Associations with Birth Weight, Birth Length and Head Circumference",
volume = "17",
number = "3",
pages = "556-565",
doi = "10.1007/s10995-012-1031-x"
}

Zeljković, A., Vekić, J., Spasić, S., Jelić-Ivanović, Z., Spasojević-Kalimanovska, V., Gojković, T., Ardalić, D., Mandić-Marković, V., Cerović, N.,& Miković, Ž.. (2013). Changes in LDL and HDL Subclasses in Normal Pregnancy and Associations with Birth Weight, Birth Length and Head Circumference. in Maternal and Child Health Journal
Springer/Plenum Publishers, New York., 17(3), 556-565.
https://doi.org/10.1007/s10995-012-1031-x

Zeljković A, Vekić J, Spasić S, Jelić-Ivanović Z, Spasojević-Kalimanovska V, Gojković T, Ardalić D, Mandić-Marković V, Cerović N, Miković Ž. Changes in LDL and HDL Subclasses in Normal Pregnancy and Associations with Birth Weight, Birth Length and Head Circumference. in Maternal and Child Health Journal. 2013;17(3):556-565.
doi:10.1007/s10995-012-1031-x .

Zeljković, Aleksandra, Vekić, Jelena, Spasić, Slavica, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, Gojković, Tamara, Ardalić, Daniela, Mandić-Marković, Vesna, Cerović, Nikola, Miković, Željko, "Changes in LDL and HDL Subclasses in Normal Pregnancy and Associations with Birth Weight, Birth Length and Head Circumference" in Maternal and Child Health Journal, 17, no. 3 (2013):556-565,
https://doi.org/10.1007/s10995-012-1031-x . .

TY  - JOUR
AU  - Stefanović, Aleksandra
AU  - Ardalić, Daniela
AU  - Kotur-Stevuljević, Jelena
AU  - Vujović, Ana
AU  - Spasić, Slavica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Jelić-Ivanović, Zorana
AU  - Mandić-Marković, Vesna
AU  - Miković, Željko
AU  - Cerović, Nikola
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1694
AB  - The purpose of the present study was to determine changes in plasma paraoxonase-1 activity (an indicator of paraoxonase phenotype) throughout normal pregnancy and its relationship with maternal oxidative stress status. The frequencies of the paraoxonase-1 phenotype in the studied population were determined using a two-substrate (paraoxon/diazoxon) activity method. As a parameter of oxidative stress status we measured the redox balance. Paraoxonase-1 activity significantly decreased at gestational week 32. In addition, the lipid profile was more atherogenic. Redox balance was significantly increased across gestational weeks. There were independent direct associations between maternal smoking habits before pregnancy, glucose concentrations and redox balance with PON1 activity in the third trimester. This study shows that pregnancy is followed by a decrease in PON1 activity and increased risk for development of cardiovascular diseases. We conclude that changes in paraoxonase-1 status during pregnancy are associated with maternal oxidative stress status and smoking habits.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Research in Microbiology
T1  - Longitudinal changes in PON1 activities, PON1 phenotype distribution and oxidative status throughout normal pregnancy
VL  - 33
IS  - 1
SP  - 20
EP  - 26
DO  - 10.1016/j.reprotox.2011.10.009
ER  - 

@article{
author = "Stefanović, Aleksandra and Ardalić, Daniela and Kotur-Stevuljević, Jelena and Vujović, Ana and Spasić, Slavica and Spasojević-Kalimanovska, Vesna and Jelić-Ivanović, Zorana and Mandić-Marković, Vesna and Miković, Željko and Cerović, Nikola",
year = "2012",
abstract = "The purpose of the present study was to determine changes in plasma paraoxonase-1 activity (an indicator of paraoxonase phenotype) throughout normal pregnancy and its relationship with maternal oxidative stress status. The frequencies of the paraoxonase-1 phenotype in the studied population were determined using a two-substrate (paraoxon/diazoxon) activity method. As a parameter of oxidative stress status we measured the redox balance. Paraoxonase-1 activity significantly decreased at gestational week 32. In addition, the lipid profile was more atherogenic. Redox balance was significantly increased across gestational weeks. There were independent direct associations between maternal smoking habits before pregnancy, glucose concentrations and redox balance with PON1 activity in the third trimester. This study shows that pregnancy is followed by a decrease in PON1 activity and increased risk for development of cardiovascular diseases. We conclude that changes in paraoxonase-1 status during pregnancy are associated with maternal oxidative stress status and smoking habits.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Research in Microbiology",
title = "Longitudinal changes in PON1 activities, PON1 phenotype distribution and oxidative status throughout normal pregnancy",
volume = "33",
number = "1",
pages = "20-26",
doi = "10.1016/j.reprotox.2011.10.009"
}

Stefanović, A., Ardalić, D., Kotur-Stevuljević, J., Vujović, A., Spasić, S., Spasojević-Kalimanovska, V., Jelić-Ivanović, Z., Mandić-Marković, V., Miković, Ž.,& Cerović, N.. (2012). Longitudinal changes in PON1 activities, PON1 phenotype distribution and oxidative status throughout normal pregnancy. in Research in Microbiology
Pergamon-Elsevier Science Ltd, Oxford., 33(1), 20-26.
https://doi.org/10.1016/j.reprotox.2011.10.009

Stefanović A, Ardalić D, Kotur-Stevuljević J, Vujović A, Spasić S, Spasojević-Kalimanovska V, Jelić-Ivanović Z, Mandić-Marković V, Miković Ž, Cerović N. Longitudinal changes in PON1 activities, PON1 phenotype distribution and oxidative status throughout normal pregnancy. in Research in Microbiology. 2012;33(1):20-26.
doi:10.1016/j.reprotox.2011.10.009 .

Stefanović, Aleksandra, Ardalić, Daniela, Kotur-Stevuljević, Jelena, Vujović, Ana, Spasić, Slavica, Spasojević-Kalimanovska, Vesna, Jelić-Ivanović, Zorana, Mandić-Marković, Vesna, Miković, Željko, Cerović, Nikola, "Longitudinal changes in PON1 activities, PON1 phenotype distribution and oxidative status throughout normal pregnancy" in Research in Microbiology, 33, no. 1 (2012):20-26,
https://doi.org/10.1016/j.reprotox.2011.10.009 . .

TY  - JOUR
AU  - Stefanović, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Vujović, Ana
AU  - Spasić, Slavica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Jelić-Ivanović, Zorana
AU  - Martinović, Jelena
AU  - Ardalić, Daniela
AU  - Mandić-Marković, Vesna
AU  - Miković, Željko
AU  - Cerović, Nikola
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1635
AB  - Background: Pregnancy is a stressful condition linked with altered lipid profile, increased oxidative stress and increased inflammation processes. The purpose of the present study was to determine the associations between those alterations with increased weight gain during pregnancy. Methods: The atherogenic index of plasma (AIP) and oxidative stress status parameters were determinated in 50 healthy and 172 pregnant women with non-complicated pregnancy. Pregnant women were divided in four groups according to body mass index (BMI) values (BMI quartiles). Results: Oxidative stress parameters were significantly lower in the control group compared with all the pregnant women quartiles. Unexpectedly, differences in oxidative stress parameters between BMI quartiles groups were not significant. The antioxidant defence parameters remained quite similar in the different BMI quartiles. Weight gain and paraoxonase-1 (PON1) activities were independently associated with increased AIP while weight gain and triglyceride concentration were found to be significant predictors of PON1 activities. Conclusions: The results of our current study indicate the association of maternal weight gain during pregnancy and altered lipid profile, elevated oxidative stress and changed antioxidative capacity of PON1. Taken together all these facts indicate possible increased risk of cardiovascular disease (CVD) development in later life if the weight gain during pregnancy is excessive.
PB  - Walter de Gruyter Gmbh, Berlin
T2  - Clinical Chemistry and Laboratory Medicine
T1  - Association of the atherogenic index of plasma and oxidative stress status with weight gain during non-complicated pregnancy
VL  - 50
IS  - 11
SP  - 2019
EP  - 2025
DO  - 10.1515/cclm-2012-0117
ER  - 

@article{
author = "Stefanović, Aleksandra and Kotur-Stevuljević, Jelena and Vujović, Ana and Spasić, Slavica and Spasojević-Kalimanovska, Vesna and Jelić-Ivanović, Zorana and Martinović, Jelena and Ardalić, Daniela and Mandić-Marković, Vesna and Miković, Željko and Cerović, Nikola",
year = "2012",
abstract = "Background: Pregnancy is a stressful condition linked with altered lipid profile, increased oxidative stress and increased inflammation processes. The purpose of the present study was to determine the associations between those alterations with increased weight gain during pregnancy. Methods: The atherogenic index of plasma (AIP) and oxidative stress status parameters were determinated in 50 healthy and 172 pregnant women with non-complicated pregnancy. Pregnant women were divided in four groups according to body mass index (BMI) values (BMI quartiles). Results: Oxidative stress parameters were significantly lower in the control group compared with all the pregnant women quartiles. Unexpectedly, differences in oxidative stress parameters between BMI quartiles groups were not significant. The antioxidant defence parameters remained quite similar in the different BMI quartiles. Weight gain and paraoxonase-1 (PON1) activities were independently associated with increased AIP while weight gain and triglyceride concentration were found to be significant predictors of PON1 activities. Conclusions: The results of our current study indicate the association of maternal weight gain during pregnancy and altered lipid profile, elevated oxidative stress and changed antioxidative capacity of PON1. Taken together all these facts indicate possible increased risk of cardiovascular disease (CVD) development in later life if the weight gain during pregnancy is excessive.",
publisher = "Walter de Gruyter Gmbh, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Association of the atherogenic index of plasma and oxidative stress status with weight gain during non-complicated pregnancy",
volume = "50",
number = "11",
pages = "2019-2025",
doi = "10.1515/cclm-2012-0117"
}

Stefanović, A., Kotur-Stevuljević, J., Vujović, A., Spasić, S., Spasojević-Kalimanovska, V., Jelić-Ivanović, Z., Martinović, J., Ardalić, D., Mandić-Marković, V., Miković, Ž.,& Cerović, N.. (2012). Association of the atherogenic index of plasma and oxidative stress status with weight gain during non-complicated pregnancy. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter Gmbh, Berlin., 50(11), 2019-2025.
https://doi.org/10.1515/cclm-2012-0117

Stefanović A, Kotur-Stevuljević J, Vujović A, Spasić S, Spasojević-Kalimanovska V, Jelić-Ivanović Z, Martinović J, Ardalić D, Mandić-Marković V, Miković Ž, Cerović N. Association of the atherogenic index of plasma and oxidative stress status with weight gain during non-complicated pregnancy. in Clinical Chemistry and Laboratory Medicine. 2012;50(11):2019-2025.
doi:10.1515/cclm-2012-0117 .

Stefanović, Aleksandra, Kotur-Stevuljević, Jelena, Vujović, Ana, Spasić, Slavica, Spasojević-Kalimanovska, Vesna, Jelić-Ivanović, Zorana, Martinović, Jelena, Ardalić, Daniela, Mandić-Marković, Vesna, Miković, Željko, Cerović, Nikola, "Association of the atherogenic index of plasma and oxidative stress status with weight gain during non-complicated pregnancy" in Clinical Chemistry and Laboratory Medicine, 50, no. 11 (2012):2019-2025,
https://doi.org/10.1515/cclm-2012-0117 . .

TY  - CONF
AU  - Franković, I.
AU  - Spasić, S.
AU  - Kotur-Stevuljević, Jelena
AU  - Ardalić, Daniela
AU  - Osap, J.
AU  - Skelić, I.
AU  - Mandić-Marković, Vesna
AU  - Miković, Željko
AU  - Cerović, Nikola
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1532
PB  - Walter de Gruyter & Co, Berlin
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Pregnancy levels of iron, total iron-binding capacity, transferin and ferritin
VL  - 49
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1532
ER  - 

@conference{
author = "Franković, I. and Spasić, S. and Kotur-Stevuljević, Jelena and Ardalić, Daniela and Osap, J. and Skelić, I. and Mandić-Marković, Vesna and Miković, Željko and Cerović, Nikola",
year = "2011",
publisher = "Walter de Gruyter & Co, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Pregnancy levels of iron, total iron-binding capacity, transferin and ferritin",
volume = "49",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1532"
}

Franković, I., Spasić, S., Kotur-Stevuljević, J., Ardalić, D., Osap, J., Skelić, I., Mandić-Marković, V., Miković, Ž.,& Cerović, N.. (2011). Pregnancy levels of iron, total iron-binding capacity, transferin and ferritin. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter & Co, Berlin., 49.
https://hdl.handle.net/21.15107/rcub_farfar_1532

Franković I, Spasić S, Kotur-Stevuljević J, Ardalić D, Osap J, Skelić I, Mandić-Marković V, Miković Ž, Cerović N. Pregnancy levels of iron, total iron-binding capacity, transferin and ferritin. in Clinical Chemistry and Laboratory Medicine. 2011;49.
https://hdl.handle.net/21.15107/rcub_farfar_1532 .

Franković, I., Spasić, S., Kotur-Stevuljević, Jelena, Ardalić, Daniela, Osap, J., Skelić, I., Mandić-Marković, Vesna, Miković, Željko, Cerović, Nikola, "Pregnancy levels of iron, total iron-binding capacity, transferin and ferritin" in Clinical Chemistry and Laboratory Medicine, 49 (2011),
https://hdl.handle.net/21.15107/rcub_farfar_1532 .

TY  - CONF
AU  - Corović, S.
AU  - Spasić, S.
AU  - Ardalić, Daniela
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
AU  - Mandić-Marković, Vesna
AU  - Miković, Željko
AU  - Cerović, Nikola
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1519
PB  - Walter de Gruyter & Co, Berlin
C3  - Clinical Chemistry and Laboratory Medicine
T1  - Serum high sensitive c-reactive protein level in normal pregnancy and his association with body mass index
VL  - 49
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1519
ER  - 

@conference{
author = "Corović, S. and Spasić, S. and Ardalić, Daniela and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna and Mandić-Marković, Vesna and Miković, Željko and Cerović, Nikola",
year = "2011",
publisher = "Walter de Gruyter & Co, Berlin",
journal = "Clinical Chemistry and Laboratory Medicine",
title = "Serum high sensitive c-reactive protein level in normal pregnancy and his association with body mass index",
volume = "49",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1519"
}

Corović, S., Spasić, S., Ardalić, D., Jelić-Ivanović, Z., Spasojević-Kalimanovska, V., Mandić-Marković, V., Miković, Ž.,& Cerović, N.. (2011). Serum high sensitive c-reactive protein level in normal pregnancy and his association with body mass index. in Clinical Chemistry and Laboratory Medicine
Walter de Gruyter & Co, Berlin., 49.
https://hdl.handle.net/21.15107/rcub_farfar_1519

Corović S, Spasić S, Ardalić D, Jelić-Ivanović Z, Spasojević-Kalimanovska V, Mandić-Marković V, Miković Ž, Cerović N. Serum high sensitive c-reactive protein level in normal pregnancy and his association with body mass index. in Clinical Chemistry and Laboratory Medicine. 2011;49.
https://hdl.handle.net/21.15107/rcub_farfar_1519 .

Corović, S., Spasić, S., Ardalić, Daniela, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, Mandić-Marković, Vesna, Miković, Željko, Cerović, Nikola, "Serum high sensitive c-reactive protein level in normal pregnancy and his association with body mass index" in Clinical Chemistry and Laboratory Medicine, 49 (2011),
https://hdl.handle.net/21.15107/rcub_farfar_1519 .